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EP0331126A3 - Process for the determination of a protein according to the principle of fluorescense polarisation immunoassay - Google Patents

Process for the determination of a protein according to the principle of fluorescense polarisation immunoassay Download PDF

Info

Publication number
EP0331126A3
EP0331126A3 EP89103527A EP89103527A EP0331126A3 EP 0331126 A3 EP0331126 A3 EP 0331126A3 EP 89103527 A EP89103527 A EP 89103527A EP 89103527 A EP89103527 A EP 89103527A EP 0331126 A3 EP0331126 A3 EP 0331126A3
Authority
EP
European Patent Office
Prior art keywords
protein
fluorescense
determination
principle
protein according
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP89103527A
Other languages
German (de)
Other versions
EP0331126B1 (en
EP0331126A2 (en
Inventor
Christian Dr.Rer.Nat. Klein
Hans-Georg Dr. Rer.Nat. Batz
Ulrich Dr.Rer.Nat Essig
Kurt Walter Dr.Rer.Nat. Naujoks
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Priority to AT89103527T priority Critical patent/ATE92634T1/en
Publication of EP0331126A2 publication Critical patent/EP0331126A2/en
Publication of EP0331126A3 publication Critical patent/EP0331126A3/en
Application granted granted Critical
Publication of EP0331126B1 publication Critical patent/EP0331126B1/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/26—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against hormones ; against hormone releasing or inhibiting factors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Food Science & Technology (AREA)
  • Endocrinology (AREA)
  • General Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

To determine a protein in a homogeneous system, a sample solution is incubated simultaneously with a) a peptide sequence which is labelled with a fluorescent compound and is composed of 6 to 14 amino acids, of which two are cysteine, which together form a disulphide bridge, the sequence corresponding to an epitope sequence of the protein to be determined and b) an antibody which is able specifically to bind both to the protein and to the peptide sequence and has molar relative affinities for the fluorescent peptide sequence of the protein and for the native protein which differ by not more than a factor of 6, and the depolarisation of the polarised light passed through the incubated solution is measured. <IMAGE>
EP89103527A 1988-02-29 1989-02-28 Process for the determination of a protein according to the principle of fluorescense polarisation immunoassay Expired - Lifetime EP0331126B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AT89103527T ATE92634T1 (en) 1988-02-29 1989-02-28 PROCEDURE FOR THE DETERMINATION OF A PROTEIN ACCORDING TO THE PRINCIPLE OF FLUORESCENCE POLARIZATION IMMUNOASSAY.

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE3806430 1988-02-29
DE3806430A DE3806430A1 (en) 1988-02-29 1988-02-29 PROCESS FOR DETERMINING A PROTEIN ACCORDING TO THE PRINCIPLE OF THE FLUORESCENCE POLARIZATION IMMUNOASSAY

Publications (3)

Publication Number Publication Date
EP0331126A2 EP0331126A2 (en) 1989-09-06
EP0331126A3 true EP0331126A3 (en) 1990-05-30
EP0331126B1 EP0331126B1 (en) 1993-08-04

Family

ID=6348449

Family Applications (1)

Application Number Title Priority Date Filing Date
EP89103527A Expired - Lifetime EP0331126B1 (en) 1988-02-29 1989-02-28 Process for the determination of a protein according to the principle of fluorescense polarisation immunoassay

Country Status (5)

Country Link
US (1) US5070025A (en)
EP (1) EP0331126B1 (en)
JP (1) JPH0210159A (en)
AT (1) ATE92634T1 (en)
DE (2) DE3806430A1 (en)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL95738A (en) * 1989-09-23 1996-11-14 Hoechst Ag Antibodies against highly conserved amino acid sequences of insulin a process for the preparation of these antibodies and the use thereof in immunoassays
DE4025725A1 (en) * 1990-08-14 1992-02-20 Boehringer Mannheim Gmbh MONOCLONAL ANTIBODIES BINDABLE WITH HUMAN AND RAT INSULIN
JPH05139242A (en) * 1991-11-19 1993-06-08 Toyota Motor Corp Body front part structure
US6482655B1 (en) * 1993-07-23 2002-11-19 University Of Utah Research Foundation Immunoassay procedure utilizing fluorogenic tracer antigens
US6632613B1 (en) 1993-06-02 2003-10-14 University Of Utah Research Foundation Compositions and kits for fluorescence polarization assay of large molecules
US6576419B1 (en) * 1993-07-23 2003-06-10 University Of Utah Research Foundation Assay procedure using fluorogenic tracers
DE69433427T2 (en) * 1993-12-09 2004-09-16 The University Of Utah Research Foundation, Salt Lake City COMPOSITIONS AND KIT FOR FLUORESCENT POLARIZING DOSING OF LARGE MOLECULES
US6054557A (en) * 1995-04-04 2000-04-25 Advanced Bioconcept (1994) Ltd. Fluorescent peptides
US5824772A (en) * 1995-04-04 1998-10-20 Advanced Bioconcept, Inc. Fluorescent somatostatin
US5693679A (en) * 1995-04-04 1997-12-02 Advanced Bioconcept, Inc. Fluorescent somatostatin
EP0820466A2 (en) * 1995-04-04 1998-01-28 Advanced Bioconcept Inc. Fluorescent peptides
US6821952B1 (en) 1995-07-20 2004-11-23 Perkinelmer Las, Inc. Fluorescent vasoactive intestinal peptide (VIP)
US6110750A (en) * 1996-06-28 2000-08-29 Sugden; Edward A. Rapid detection method of Mycobacterium bovis by fluorescence polarization
EP1036192B1 (en) 1997-12-05 2003-02-26 PHARMACIA &amp; UPJOHN COMPANY Fluorescence-based high throughput screening assays for protein kinases and phosphatases
WO2001016600A1 (en) * 1999-08-31 2001-03-08 Mitsubishi Chemical Corporation Method of analyzing mutual interaction between protein and molecule
NO20006130D0 (en) * 2000-12-01 2000-12-01 Erling Sundrehagen Reagent and assay method
US20030060695A1 (en) * 2001-03-07 2003-03-27 Connelly Patrick R. Implantable artificial organ devices
US20040014030A1 (en) * 2002-07-18 2004-01-22 Lili Arabshahi Enzymatic assay of HIV protease inhibitors
US6821744B2 (en) 2002-10-29 2004-11-23 Roche Diagnostics Operations, Inc. Method, assay, and kit for quantifying HIV protease inhibitors
US7256008B2 (en) * 2006-01-06 2007-08-14 Abbott Laboratories Determination of concentration of FK778 by competitive immunoassay
US8962677B2 (en) * 2007-07-12 2015-02-24 Acumen Pharmaceuticals, Inc. Methods of restoring cognitive ability using non-peptidic compounds
US9006283B2 (en) * 2007-07-12 2015-04-14 Acumen Pharmaceuticals, Inc. Methods of modifying amyloid β oligomers using non-peptidic compounds
US20110098309A1 (en) * 2007-07-12 2011-04-28 Acumen Pharmaceuticals, Inc. Methods of inhibiting the formation of amyloid-beta diffusable ligands using acylhydrazide compounds
CA2707309A1 (en) 2007-12-18 2009-06-25 Acumen Pharmaceuticals, Inc. Novel addl receptor polypeptides, polynucleotides and host cells for recombinant production
KR101366353B1 (en) * 2009-07-21 2014-02-21 세키스이 메디칼 가부시키가이샤 Insulin measurement method

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0210410A1 (en) * 1985-07-22 1987-02-04 Abbott Laboratories Fluorescence polarization immunoassay and reagents for measurement of C-reactive protein

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3411472A1 (en) * 1983-03-29 1984-10-18 The United States Of America, Represented By The Secretary, U.S. Department Of Commerce, Springfield, Va. Method for determining the presence of insulin, antiinsulin antibody, method for determining the human insulin level and method for determining human insulin
US4681859A (en) * 1984-09-21 1987-07-21 Ortho Diagnostic Systems Inc. Fluorescence polarization immunoassay for heavy antigens
JPS6280048A (en) * 1985-10-04 1987-04-13 Toshiba Corp Recorder

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0210410A1 (en) * 1985-07-22 1987-02-04 Abbott Laboratories Fluorescence polarization immunoassay and reagents for measurement of C-reactive protein

Also Published As

Publication number Publication date
DE58905081D1 (en) 1993-09-09
EP0331126B1 (en) 1993-08-04
DE3806430A1 (en) 1989-09-07
EP0331126A2 (en) 1989-09-06
JPH0210159A (en) 1990-01-12
ATE92634T1 (en) 1993-08-15
US5070025A (en) 1991-12-03

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