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EA201592169A1 - Рекомбинантные микроорганизмы, приводящие к увеличению потока через путь ферментации - Google Patents

Рекомбинантные микроорганизмы, приводящие к увеличению потока через путь ферментации

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Publication number
EA201592169A1
EA201592169A1 EA201592169A EA201592169A EA201592169A1 EA 201592169 A1 EA201592169 A1 EA 201592169A1 EA 201592169 A EA201592169 A EA 201592169A EA 201592169 A EA201592169 A EA 201592169A EA 201592169 A1 EA201592169 A1 EA 201592169A1
Authority
EA
Eurasian Patent Office
Prior art keywords
increasing
fermentation
flow
enzyming
way
Prior art date
Application number
EA201592169A
Other languages
English (en)
Inventor
Майкл Копке
Александер Пол Мюллер
Original Assignee
Ланцатек Нью Зилэнд Лимитед
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
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Application filed by Ланцатек Нью Зилэнд Лимитед filed Critical Ланцатек Нью Зилэнд Лимитед
Publication of EA201592169A1 publication Critical patent/EA201592169A1/ru

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/065—Ethanol, i.e. non-beverage with microorganisms other than yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01001—Alcohol dehydrogenase (1.1.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/0101—Acetaldehyde dehydrogenase (acetylating) (1.2.1.10)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/07—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with an iron-sulfur protein as acceptor (1.2.7)
    • C12Y102/07001—Pyruvate synthase (1.2.7.1), i.e. pyruvate ferredoxin oxidoreductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/07—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with an iron-sulfur protein as acceptor (1.2.7)
    • C12Y102/07005—Aldehyde ferredoxin oxidoreductase (1.2.7.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y201/00—Transferases transferring one-carbon groups (2.1)
    • C12Y201/01—Methyltransferases (2.1.1)
    • C12Y201/01196—Cobalt-precorrin-7 (C15)-methyltransferase (decarboxylating) (2.1.1.196)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Согласно настоящему изобретению предложены способы увеличения выработки продуктов ферментации с помощью увеличения потока через путь ферментации путем оптимизации ферментативных реакций. В частности, настоящее изобретение относится к идентификации ферментов и/или кофакторов, вовлеченных в метаболические узкие места в путях ферментации, и ферментации содержащего СО субстрата с помощью рекомбинантного карбоксидотрофного микроорганизма класса Clostridia, приспособленного для того, чтобы приводить к повышенной активности одного или более из указанных ферментов или повышенной доступности одного или более из указанных кофакторов по сравнению с исходным микроорганизмом.
EA201592169A 2013-06-05 2014-06-05 Рекомбинантные микроорганизмы, приводящие к увеличению потока через путь ферментации EA201592169A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361831591P 2013-06-05 2013-06-05
PCT/US2014/041188 WO2014197746A1 (en) 2013-06-05 2014-06-05 Recombinant microorganisms exhibiting increased flux through a fermentation pathway

Publications (1)

Publication Number Publication Date
EA201592169A1 true EA201592169A1 (ru) 2016-04-29

Family

ID=52008601

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201592169A EA201592169A1 (ru) 2013-06-05 2014-06-05 Рекомбинантные микроорганизмы, приводящие к увеличению потока через путь ферментации

Country Status (10)

Country Link
US (1) US20150079650A1 (ru)
EP (1) EP3004362A4 (ru)
JP (1) JP2016520325A (ru)
KR (1) KR20160018568A (ru)
CN (1) CN105283554A (ru)
AU (2) AU2014274838A1 (ru)
BR (1) BR112015030208A2 (ru)
CA (1) CA2914003C (ru)
EA (1) EA201592169A1 (ru)
WO (1) WO2014197746A1 (ru)

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CN104515851B (zh) * 2015-01-08 2016-09-14 河南农业大学 一种直接测定厌氧微生物内氢化酶或一氧化碳脱氢酶活性的方法与装置
CN105624182A (zh) * 2015-12-15 2016-06-01 重庆大学 生产四氢叶酸的重组质粒、基因工程菌株构建及应用
CN108603178A (zh) * 2016-01-06 2018-09-28 株式会社西化学 Co水合酶以及使用其产生甲酸的方法
KR102514023B1 (ko) * 2016-05-14 2023-03-23 란자테크, 인크. 알데히드:페레독신 옥시도리덕타제 활성이 변경된 미생물 및 관련 방법
CN108330094B (zh) * 2018-01-10 2020-04-21 上海晶诺生物科技有限公司 一种产烟酸的游离型重组耻垢分枝杆菌及其构建方法
CN109810991B (zh) * 2019-03-02 2021-11-12 昆明理工大学 二氢蝶酸合酶基因folP的用途
BR112021018090A2 (pt) 2019-03-20 2021-11-23 Global Bioenergies Organismo ou micro-organismo recombinante, uso do organismo ou micro-organismo recombinante e método para a produção de isobuteno
CN111089914A (zh) * 2019-11-07 2020-05-01 河北首朗新能源科技有限公司 微生物发酵生产乙醇中代谢物的高效液相检测方法
CN111235169A (zh) * 2020-02-03 2020-06-05 昆明理工大学 一种GTP环化水解酶I基因folE及应用
CN114480562B (zh) * 2022-01-27 2023-09-19 西南医科大学附属医院 一种基于pfor酶活性导向的fmt供体筛选方法及其应用

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Also Published As

Publication number Publication date
KR20160018568A (ko) 2016-02-17
EP3004362A4 (en) 2017-01-11
CN105283554A (zh) 2016-01-27
EP3004362A1 (en) 2016-04-13
AU2018204112A1 (en) 2018-06-28
BR112015030208A2 (pt) 2017-08-22
WO2014197746A1 (en) 2014-12-11
JP2016520325A (ja) 2016-07-14
US20150079650A1 (en) 2015-03-19
CA2914003C (en) 2018-01-02
CA2914003A1 (en) 2014-12-11
AU2014274838A1 (en) 2015-12-24

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