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DK3310914T3 - Chymosinvarianter med forbedrede egenskaber - Google Patents

Chymosinvarianter med forbedrede egenskaber Download PDF

Info

Publication number
DK3310914T3
DK3310914T3 DK16751481.9T DK16751481T DK3310914T3 DK 3310914 T3 DK3310914 T3 DK 3310914T3 DK 16751481 T DK16751481 T DK 16751481T DK 3310914 T3 DK3310914 T3 DK 3310914T3
Authority
DK
Denmark
Prior art keywords
improved properties
chymosin variants
chymosin
variants
properties
Prior art date
Application number
DK16751481.9T
Other languages
English (en)
Inventor
Christian Jaeckel
Martin Lund
Enikö Fodor Hansen
Lone Riisberg
Iben Jeppesen
Den Brink Johannes Maarten Van
Original Assignee
Chr Hansen As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chr Hansen As filed Critical Chr Hansen As
Application granted granted Critical
Publication of DK3310914T3 publication Critical patent/DK3310914T3/da

Links

Classifications

    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING OR TREATMENT THEREOF
    • A23C19/00Cheese; Cheese preparations; Making thereof
    • A23C19/02Making cheese curd
    • A23C19/032Making cheese curd characterised by the use of specific microorganisms, or enzymes of microbial origin
    • A23C19/0326Rennet produced by fermentation, e.g. microbial rennet; Rennet produced by genetic engineering
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING OR TREATMENT THEREOF
    • A23C19/00Cheese; Cheese preparations; Making thereof
    • A23C19/06Treating cheese curd after whey separation; Products obtained thereby
    • A23C19/061Addition of, or treatment with, microorganisms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING OR TREATMENT THEREOF
    • A23C19/00Cheese; Cheese preparations; Making thereof
    • A23C19/06Treating cheese curd after whey separation; Products obtained thereby
    • A23C19/068Particular types of cheese
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/189Enzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478Aspartic endopeptidases (3.4.23)
    • C12N9/6483Chymosin (3.4.23.4), i.e. rennin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y304/00Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/23Aspartic endopeptidases (3.4.23)
    • C12Y304/23004Chymosin (3.4.23.4), i.e. rennin

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Animal Husbandry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • General Preparation And Processing Of Foods (AREA)
  • Fodder In General (AREA)
  • Dairy Products (AREA)
  • Cosmetics (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Jellies, Jams, And Syrups (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
DK16751481.9T 2015-06-22 2016-06-22 Chymosinvarianter med forbedrede egenskaber DK3310914T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP15173099 2015-06-22
PCT/EP2016/064414 WO2016207214A1 (en) 2015-06-22 2016-06-22 Variants of chymosin with improved properties

Publications (1)

Publication Number Publication Date
DK3310914T3 true DK3310914T3 (da) 2022-06-20

Family

ID=53496438

Family Applications (1)

Application Number Title Priority Date Filing Date
DK16751481.9T DK3310914T3 (da) 2015-06-22 2016-06-22 Chymosinvarianter med forbedrede egenskaber

Country Status (13)

Country Link
US (1) US11174473B2 (da)
EP (2) EP4079849A1 (da)
JP (1) JP6856551B2 (da)
KR (1) KR102612929B1 (da)
CN (1) CN107849550B (da)
AR (1) AR105620A1 (da)
AU (1) AU2016282825B2 (da)
CA (1) CA2989488A1 (da)
DK (1) DK3310914T3 (da)
MX (1) MX2017016623A (da)
NZ (1) NZ738238A (da)
RU (1) RU2740317C2 (da)
WO (1) WO2016207214A1 (da)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2874214C (en) 2012-05-25 2021-02-16 Chr. Hansen A/S Variants of chymosin with improved milk-clotting properties
BR112016017502B1 (pt) 2014-02-26 2023-10-24 Chr. Hansen A/S Variantes de polipeptídeo de quimosina isoladas método para a produção das mesmas e método para a fabricação de um produto de alimentação humana ou animal
MX2017016623A (es) 2015-06-22 2018-05-15 Chr Hansen As Variantes de quimosina con propiedades mejoradas.
AR105867A1 (es) * 2015-08-31 2017-11-15 Chr Hansen As VARIANTES DE QUIMOSINA CON PROPIEDADES MEJORADAS DE a-S1-CASEÍNA
MX2018013924A (es) 2016-05-19 2019-03-28 Chr Hansen As Variantes de quimosina con propiedades mejoradas de coagulacion de leche.
CN109688825A (zh) * 2016-05-19 2019-04-26 科·汉森有限公司 具有改善的凝乳特性的凝乳酶的变体
EP4373285A1 (en) 2021-07-20 2024-05-29 Chr. Hansen A/S Composition for clotting milk, methods and uses thereof
WO2023194285A2 (en) 2022-04-06 2023-10-12 Chr. Hansen A/S Aspartic protease, methods and uses thereof

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3549390A (en) * 1968-09-09 1970-12-22 Miles Lab Milk-clotting enzyme product and process therefor
NO841276L (no) 1983-03-31 1984-10-01 Codon Genetic Eng Lab Rekombinant dna-koding for et polypeptid som utviser melkesammenloepende aktivitet
US7390936B1 (en) 1999-08-23 2008-06-24 Sembiosys Genetics Inc. Commercial production of chymosin in plants
RU2192137C2 (ru) 2000-08-07 2002-11-10 Открытое акционерное общество "Уфамолагропром" Способ производства сыра
DE60143406D1 (de) 2000-11-06 2010-12-16 Chr Hansen As Verfahren zur herstellung von nicht aus dem rind stammendem chymosin und dessen anwendung
RU2296160C2 (ru) * 2001-02-23 2007-03-27 ДСМ Ай Пи ЭССЕТС Б.В. Новые гены, кодирующие новые протеолитические ферменты
EP1608947A4 (en) 2002-10-02 2009-06-17 Catalyst Biosciences Inc PROCESS FOR PRODUCING AND TESTING FOR PROTEASES WITH CHANGED SPECIFICITY
KR20060034650A (ko) 2003-06-27 2006-04-24 바이오렌 인코포레이티드 룩-스루 돌연변이
EP1745068A4 (en) 2004-03-30 2009-11-04 Sudershan Biotech Ltd RECOMBINANT CALM CHYMOSIN AND PROCESS FOR PRODUCING THE SAME
EP2117331A1 (en) 2007-02-13 2009-11-18 Chr. Hansen A/S Coagulation of milk
JP2010046034A (ja) 2008-08-22 2010-03-04 Toyota Central R&D Labs Inc 変異体のスクリーニング方法
EP2411512B1 (en) 2009-03-24 2013-11-20 Meito Sangyo Co., Ltd. Improved type milk-clotting protease derived from a microorganism
US10167463B2 (en) * 2012-05-03 2019-01-01 Dsm Ip Assets B.V. Modified chymosin polypeptides
CA2874214C (en) 2012-05-25 2021-02-16 Chr. Hansen A/S Variants of chymosin with improved milk-clotting properties
KR20150062717A (ko) * 2013-11-29 2015-06-08 롯데푸드 주식회사 발효 크림치즈 제조방법
BR112016017502B1 (pt) 2014-02-26 2023-10-24 Chr. Hansen A/S Variantes de polipeptídeo de quimosina isoladas método para a produção das mesmas e método para a fabricação de um produto de alimentação humana ou animal
CN107208082A (zh) 2015-02-10 2017-09-26 科.汉森有限公司 具有改善的凝乳特性的凝乳酶共混物
MX2017016623A (es) 2015-06-22 2018-05-15 Chr Hansen As Variantes de quimosina con propiedades mejoradas.
AR105867A1 (es) 2015-08-31 2017-11-15 Chr Hansen As VARIANTES DE QUIMOSINA CON PROPIEDADES MEJORADAS DE a-S1-CASEÍNA
CN109688825A (zh) * 2016-05-19 2019-04-26 科·汉森有限公司 具有改善的凝乳特性的凝乳酶的变体
MX2018013924A (es) 2016-05-19 2019-03-28 Chr Hansen As Variantes de quimosina con propiedades mejoradas de coagulacion de leche.

Also Published As

Publication number Publication date
AU2016282825A1 (en) 2018-01-04
NZ738238A (en) 2024-07-05
MX2017016623A (es) 2018-05-15
RU2017144572A3 (da) 2020-01-27
AU2016282825B2 (en) 2022-11-17
JP2018525974A (ja) 2018-09-13
KR20180021098A (ko) 2018-02-28
CN107849550A (zh) 2018-03-27
AR105620A1 (es) 2017-10-25
US11174473B2 (en) 2021-11-16
WO2016207214A1 (en) 2016-12-29
CN107849550B (zh) 2021-09-03
US20180187179A1 (en) 2018-07-05
RU2017144572A (ru) 2019-07-22
RU2740317C2 (ru) 2021-01-13
EP4079849A1 (en) 2022-10-26
EP3310914B1 (en) 2022-04-06
CA2989488A1 (en) 2016-12-29
EP3310914A1 (en) 2018-04-25
KR102612929B1 (ko) 2023-12-12
JP6856551B2 (ja) 2021-04-07
BR112017027536A2 (pt) 2018-09-11

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