[go: up one dir, main page]
More Web Proxy on the site http://driver.im/

DK3388519T3 - Vesikulær adapter og anvendelser deraf i konstruktion af nukleinsyrebibliotek og sekvensering - Google Patents

Vesikulær adapter og anvendelser deraf i konstruktion af nukleinsyrebibliotek og sekvensering Download PDF

Info

Publication number
DK3388519T3
DK3388519T3 DK18174793.2T DK18174793T DK3388519T3 DK 3388519 T3 DK3388519 T3 DK 3388519T3 DK 18174793 T DK18174793 T DK 18174793T DK 3388519 T3 DK3388519 T3 DK 3388519T3
Authority
DK
Denmark
Prior art keywords
vesicular
adapters
sequence
applications
construction
Prior art date
Application number
DK18174793.2T
Other languages
English (en)
Inventor
Yuan Jiang
Jing Guo
Xiaojun Ji
Chunyu Geng
Kai Tian
Xia Zhao
Huaiqian Xu
Wenwei Zhang
Hui Jiang
Radoje Drmanac
Original Assignee
Mgi Tech Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mgi Tech Co Ltd filed Critical Mgi Tech Co Ltd
Application granted granted Critical
Publication of DK3388519T3 publication Critical patent/DK3388519T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/06—Enzymes or microbial cells immobilised on or in an organic carrier attached to the carrier via a bridging agent
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/14—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/00527—Sheets
    • B01J2219/00529—DNA chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/537—Detection characterised by immobilisation to a surface characterised by the capture oligonucleotide acting as a primer

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Virology (AREA)
  • Structural Engineering (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
DK18174793.2T 2014-09-12 2014-11-21 Vesikulær adapter og anvendelser deraf i konstruktion af nukleinsyrebibliotek og sekvensering DK3388519T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
PCT/CN2014/086418 WO2016037358A1 (zh) 2014-09-12 2014-09-12 分离的寡核苷酸及其在核酸测序中的用途
EP14901593.5A EP3192877B1 (en) 2014-09-12 2014-11-21 Vesicular adaptor and uses thereof in nucleic acid library construction and sequencing

Publications (1)

Publication Number Publication Date
DK3388519T3 true DK3388519T3 (da) 2019-08-05

Family

ID=55458278

Family Applications (4)

Application Number Title Priority Date Filing Date
DK14901591.9T DK3192869T3 (da) 2014-09-12 2014-09-12 Isoleret oligonukleotid og anvendelse deraf i nukleinsyresekvensering
DK14901597.6T DK3192900T3 (da) 2014-09-12 2014-10-14 Metode til konstruktion af et bibliotek indeholdende enkeltstrengede cykliske nukleinsyrer og forbindelser deraf
DK14901593.5T DK3192877T3 (da) 2014-09-12 2014-11-21 Vesikulær adapter og anvendelser deraf i nukleinsyrebibliotekskonstruktion og -sekvensering
DK18174793.2T DK3388519T3 (da) 2014-09-12 2014-11-21 Vesikulær adapter og anvendelser deraf i konstruktion af nukleinsyrebibliotek og sekvensering

Family Applications Before (3)

Application Number Title Priority Date Filing Date
DK14901591.9T DK3192869T3 (da) 2014-09-12 2014-09-12 Isoleret oligonukleotid og anvendelse deraf i nukleinsyresekvensering
DK14901597.6T DK3192900T3 (da) 2014-09-12 2014-10-14 Metode til konstruktion af et bibliotek indeholdende enkeltstrengede cykliske nukleinsyrer og forbindelser deraf
DK14901593.5T DK3192877T3 (da) 2014-09-12 2014-11-21 Vesikulær adapter og anvendelser deraf i nukleinsyrebibliotekskonstruktion og -sekvensering

Country Status (9)

Country Link
US (4) US9890375B2 (da)
EP (5) EP3192869B1 (da)
JP (3) JP6483249B2 (da)
CN (3) CN107075513B (da)
AU (3) AU2014406026B2 (da)
DK (4) DK3192869T3 (da)
ES (5) ES2726149T3 (da)
HK (2) HK1215721A1 (da)
WO (4) WO2016037358A1 (da)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014093330A1 (en) 2012-12-10 2014-06-19 Clearfork Bioscience, Inc. Methods for targeted genomic analysis
US9328382B2 (en) 2013-03-15 2016-05-03 Complete Genomics, Inc. Multiple tagging of individual long DNA fragments
EP3604544B1 (en) 2014-01-31 2021-12-15 Swift Biosciences, Inc. Improved methods for processing dna substrates
US10208338B2 (en) 2014-03-03 2019-02-19 Swift Biosciences, Inc. Enhanced adaptor ligation
WO2016037361A1 (zh) * 2014-09-12 2016-03-17 深圳华大基因科技有限公司 试剂盒及其在核酸测序中的用途
EP3208345B1 (en) * 2014-10-14 2019-12-04 MGI Tech Co., Ltd. One-stop treatment method for breaking nucleic acid by means of transposase, and reagent
US10221448B2 (en) 2015-03-06 2019-03-05 Pillar Biosciences Inc. Selective amplification of overlapping amplicons
MX2018005858A (es) * 2015-11-11 2019-02-20 Resolution Bioscience Inc Construccion de alta eficacia de bibliotecas de adn.
EP3485035B1 (en) 2016-07-15 2024-07-03 The Regents of the University of California Methods of producing nucleic acid libraries
ES2910080T3 (es) * 2016-07-18 2022-05-11 Hoffmann La Roche Moldes asimétricos y procedimiento asimétrico de secuenciación de ácido nucleico
CN109642230A (zh) 2016-08-16 2019-04-16 加利福尼亚大学董事会 通过杂交找到低丰度序列(flash)的方法
MX2019002093A (es) 2016-08-25 2019-06-20 Resolution Bioscience Inc Métodos para la detección de cambios de copia genómica en muestras de adn.
EP3510174A4 (en) * 2016-09-07 2020-02-19 Baylor College of Medicine CLINICAL APPLICATION OF ACELLULAR DNA ANALYSIS TECHNOLOGIES TO NON-INVASIVE PRENATAL DIAGNOSIS AND OTHER LIQUID BIOPSIES
CN109689872B (zh) * 2016-11-21 2022-12-23 深圳华大智造科技股份有限公司 一种dna末端修复与加a的方法
EP3559269B1 (en) * 2016-12-20 2020-09-16 F. Hoffmann-La Roche AG Single stranded circular dna libraries for circular consensus sequencing
IL263118B2 (en) * 2017-02-21 2023-12-01 Illumina Inc Tegumentation using fixed transpososomes with linkers
WO2019053132A1 (en) * 2017-09-14 2019-03-21 F. Hoffmann-La Roche Ag NEW METHOD FOR THE GENERATION OF CIRCULAR SINGLE STRAND DNA LIBRARIES
CN109750086B (zh) * 2017-11-06 2022-08-02 深圳华大智造科技股份有限公司 单链环状文库的构建方法
AU2019207900A1 (en) 2018-01-12 2020-07-09 Claret Bioscience, Llc Methods and compositions for analyzing nucleic acid
EP3749782B1 (en) * 2018-02-05 2022-11-02 F. Hoffmann-La Roche AG Generation of single-stranded circular dna templates for single molecule sequencing
ES2947437T3 (es) 2018-05-08 2023-08-09 Mgi Tech Co Ltd Creación de Códigos de barra compartidos en el ADN, en un solo tubo con perlas, para la secuenciación, haplotipado y ensamblaje preciso y rentable
WO2019236726A1 (en) 2018-06-06 2019-12-12 The Regents Of The University Of California Methods of producing nucleic acid libraries and compositions and kits for practicing same
WO2019241290A1 (en) * 2018-06-12 2019-12-19 Accuragen Holdings Limited Methods and compositions for forming ligation products
CN109023536A (zh) * 2018-06-28 2018-12-18 河南师范大学 一种植物降解组文库构建方法
CN110734967B (zh) * 2018-07-19 2023-02-17 深圳华大智造科技股份有限公司 一种接头组合物及其应用
EP3918088B1 (en) 2019-01-29 2024-03-13 MGI Tech Co., Ltd. High coverage stlfr
CN112342627B (zh) * 2019-08-09 2024-07-23 深圳市真迈生物科技有限公司 一种核酸文库的制备方法及测序方法
CN111139533B (zh) * 2019-09-27 2021-11-09 上海英基生物科技有限公司 稳定性增加的测序文库接头
RU2746960C1 (ru) * 2020-09-23 2021-04-22 Федеральное государственное автономное образовательное учреждение высшего образования "Российский национальный исследовательский медицинский университет имени Н.И. Пирогова" Министерства здравоохранения Российской Федерации (ФГАОУ ВО РНИМУ им. Н.И. Пирогова Минздрава России) Способ создания кольцевой формы NGS библиотеки для высокопроизводительного секвенирования на платформах технологии DNBSEQ
CN116042767A (zh) * 2021-10-28 2023-05-02 深圳华大生命科学研究院 测序文库的构建方法及试剂盒
CN113943764B (zh) * 2021-12-20 2022-03-04 中国海洋大学 一种制备双链rna的方法
WO2023240611A1 (zh) * 2022-06-17 2023-12-21 深圳华大智造科技股份有限公司 单链核酸环状文库的建库以及测序方法

Family Cites Families (47)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1195872C (zh) * 1996-06-06 2005-04-06 林克斯治疗公司 通过连接经编码的衔接子进行测序
CA2360342A1 (en) 1999-12-02 2001-06-07 Molecular Staging Inc. Generation of single-strand circular dna from linear self-annealing segments
JP4480715B2 (ja) * 2003-01-29 2010-06-16 ４５４ コーポレーション 二重末端シーケンシング
US20080318215A1 (en) * 2005-12-20 2008-12-25 Ming-Sheng Lee Apparatus, methods and products for detecting genetic mutation
US20070172839A1 (en) * 2006-01-24 2007-07-26 Smith Douglas R Asymmetrical adapters and methods of use thereof
EP2602321B1 (en) 2006-05-31 2017-08-23 Sequenom, Inc. Methods and compositions for the extraction and amplification of nucleic acid from a sample
WO2008015396A2 (en) * 2006-07-31 2008-02-07 Solexa Limited Method of library preparation avoiding the formation of adaptor dimers
US7910302B2 (en) * 2006-10-27 2011-03-22 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
US20090111706A1 (en) * 2006-11-09 2009-04-30 Complete Genomics, Inc. Selection of dna adaptor orientation by amplification
US8518640B2 (en) * 2007-10-29 2013-08-27 Complete Genomics, Inc. Nucleic acid sequencing and process
WO2009061840A1 (en) * 2007-11-05 2009-05-14 Complete Genomics, Inc. Methods and oligonucleotide designs for insertion of multiple adaptors employing selective methylation
SG193776A1 (en) * 2007-12-05 2013-10-30 Complete Genomics Inc Efficient base determination in sequencing reactions
BRPI0908734A2 (pt) * 2008-03-17 2015-07-28 Expressive Res Bv Métodos para a identificação de dna genômico em uma amostra e para a indentificação de polimorfismos, e, kit
CA2719747C (en) * 2008-03-28 2018-02-20 Pacific Biosciences Of California, Inc. Compositions and methods for nucleic acid sequencing
WO2009133466A2 (en) * 2008-04-30 2009-11-05 Population Genetics Technologies Ltd. Asymmetric adapter library construction
EP2334802A4 (en) * 2008-09-09 2012-01-25 Life Technologies Corp METHODS OF GENERATING SPECIFIC LIBRARIES OF GENES
US8383345B2 (en) * 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
DK3272879T3 (da) 2008-10-24 2019-08-26 Epicentre Tech Corporation Transposonendesammensætninger og fremgangsmåder til modificering af nukleinsyrer
US9080211B2 (en) * 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
WO2010133972A1 (en) * 2009-05-22 2010-11-25 Population Genetics Technologies Ltd Sorting asymmetrically tagged nucleic acids by selective primer extension
US9023769B2 (en) * 2009-11-30 2015-05-05 Complete Genomics, Inc. cDNA library for nucleic acid sequencing
JP2013514079A (ja) * 2009-12-17 2013-04-25 キージーン・エン・フェー 制限酵素に基づく全ゲノムシーケンシング
WO2011112718A1 (en) * 2010-03-10 2011-09-15 Ibis Biosciences, Inc. Production of single-stranded circular nucleic acid
US9096952B2 (en) * 2010-06-24 2015-08-04 Population Genetics Technologies Ltd. Methods and compositions for polynucleotide library production, immortalization and region of interest extraction
WO2012000445A1 (zh) * 2010-06-30 2012-01-05 深圳华大基因科技有限公司 新的的pcr测序方法及其在hla基因分型中的应用
CN102409049B (zh) * 2010-09-21 2013-10-23 深圳华大基因科技服务有限公司 一种基于pcr的dna标签文库构建方法
CN102409045B (zh) * 2010-09-21 2013-09-18 深圳华大基因科技服务有限公司 一种基于dna接头连接的标签文库构建方法及其所使用标签和标签接头
CN102534811B (zh) * 2010-12-16 2013-11-20 深圳华大基因科技服务有限公司 一种dna文库及其制备方法、一种dna测序方法和装置
CN102181943B (zh) 2011-03-02 2013-06-05 中山大学 一种配对双末端文库构建方法及用该文库进行基因组测序的方法
CN112592960B (zh) * 2011-05-20 2024-08-27 富鲁达公司 核酸编码反应
CN102296065B (zh) * 2011-08-04 2013-05-15 盛司潼 用于构建测序文库的系统与方法
CN103014137B (zh) 2011-09-22 2015-01-07 深圳华大基因科技服务有限公司 一种分析基因表达定量的方法
EP2769007B1 (en) * 2011-10-19 2016-12-07 Nugen Technologies, Inc. Compositions and methods for directional nucleic acid amplification and sequencing
CN103103624B (zh) 2011-11-15 2014-12-31 深圳华大基因科技服务有限公司 高通量测序文库的构建方法及其应用
CN104093890B (zh) * 2012-01-26 2016-04-20 纽亘技术公司 用于靶向核酸序列富集和高效文库产生的组合物和方法
NO2694769T3 (da) * 2012-03-06 2018-03-03
CN108018343B (zh) * 2012-05-10 2023-01-03 通用医疗公司 用于测定核苷酸序列的方法
CN102703426A (zh) 2012-05-21 2012-10-03 吴江汇杰生物科技有限公司 构建核酸库的方法、试剂及试剂盒
GB2518078B (en) * 2012-06-18 2015-04-29 Nugen Technologies Inc Compositions and methods for negative selection of non-desired nucleic acid sequences
CN102703432A (zh) 2012-07-11 2012-10-03 烟台博诺生物科技有限公司 构建核酸库的方法、试剂及试剂盒
US9644199B2 (en) * 2012-10-01 2017-05-09 Agilent Technologies, Inc. Immobilized transposase complexes for DNA fragmentation and tagging
WO2014086037A1 (zh) * 2012-12-07 2014-06-12 深圳华大基因科技服务有限公司 构建核酸测序文库的方法及其应用
US9683230B2 (en) * 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
CN103667273B (zh) * 2013-12-05 2016-01-20 北京诺禾致源生物信息科技有限公司 双链接头、其应用及构建末端配对dna文库的方法
EP3604544B1 (en) * 2014-01-31 2021-12-15 Swift Biosciences, Inc. Improved methods for processing dna substrates
CN107075508B (zh) 2014-11-21 2021-03-16 深圳华大智造科技有限公司 使用鼓泡状接头元件构建测序文库的方法
CN107124888B (zh) 2014-11-21 2021-08-06 深圳华大智造科技股份有限公司 鼓泡状接头元件和使用其构建测序文库的方法

Also Published As

Publication number Publication date
EP3192869A1 (en) 2017-07-19
EP3192900B1 (en) 2018-09-05
JP6483249B2 (ja) 2019-03-13
ES2708804T3 (es) 2019-04-11
US10995367B2 (en) 2021-05-04
WO2016037358A1 (zh) 2016-03-17
US20200080140A1 (en) 2020-03-12
AU2014405969B2 (en) 2018-03-29
ES2738480T3 (es) 2020-01-23
CN107075513A (zh) 2017-08-18
HK1217726A1 (zh) 2017-01-20
ES2726149T3 (es) 2019-10-02
HK1215721A1 (zh) 2016-09-09
AU2014405969A1 (en) 2017-04-06
DK3192869T3 (da) 2019-05-20
CN106795514A (zh) 2017-05-31
EP3192877B1 (en) 2019-01-09
EP3388519B1 (en) 2019-07-10
US20170275609A1 (en) 2017-09-28
US20170356039A1 (en) 2017-12-14
AU2014406026A1 (en) 2017-04-06
WO2016037389A1 (en) 2016-03-17
EP3191630A1 (en) 2017-07-19
EP3192877A4 (en) 2017-07-19
ES2689353T3 (es) 2018-11-13
JP6679576B2 (ja) 2020-04-15
AU2014405991B2 (en) 2018-11-15
DK3192900T3 (da) 2018-10-22
AU2014406026B2 (en) 2018-08-23
US10023906B2 (en) 2018-07-17
US20180044667A1 (en) 2018-02-15
EP3192877A1 (en) 2017-07-19
AU2014405991A1 (en) 2017-04-06
EP3192900A4 (en) 2017-08-02
DK3192877T3 (da) 2019-03-04
WO2016037416A1 (zh) 2016-03-17
US9890375B2 (en) 2018-02-13
ES2682068T3 (es) 2018-09-18
EP3191630A4 (en) 2017-07-19
EP3191630B1 (en) 2018-06-27
CN107075513B (zh) 2020-11-03
US10544451B2 (en) 2020-01-28
CN107075731B (zh) 2019-11-08
EP3192869A4 (en) 2018-03-21
JP2017526725A (ja) 2017-09-14
EP3192900A1 (en) 2017-07-19
EP3192869B1 (en) 2019-03-27
JP6438126B2 (ja) 2018-12-12
CN107075731A (zh) 2017-08-18
CN106795514B (zh) 2020-05-05
JP2017532028A (ja) 2017-11-02
JP2017528138A (ja) 2017-09-28
WO2016037394A1 (zh) 2016-03-17
EP3388519A1 (en) 2018-10-17

Similar Documents

Publication Publication Date Title
DK3388519T3 (da) Vesikulær adapter og anvendelser deraf i konstruktion af nukleinsyrebibliotek og sekvensering
DK3553180T3 (da) Fremgangsmåde til konstruktion af enkeltcellesekventeringsbibliotek og anvendelse deraf
DK3185854T3 (da) Injicerbare opslæmninger og fremgangsmåder til fremstilling og anvendelse af disse
DK3690056T3 (da) Fremgangsmåder og produkter til fremstilling og indgivelse af nukleinsyre
DK3637269T3 (da) Forvaltning af lagerenheder
DK3480213T3 (da) Syntac-polypeptider og anvendelser deraf
DK3191993T3 (da) Detektion af repeatudvidelser med short read-sekventeringsdata
DK3350201T3 (da) Manipulerede fytaser og fremgangsmåder til anvendelse af samme
DK3374525T3 (da) Højeffektiv konstruktion af dna-biblioteker
DK3364958T3 (da) Modulatorer af sestrin-gator2-interaktion og anvendelser deraf
DK3209773T3 (da) Prolintolerante tripeptidylpeptidaser og anvendelser deraf
DK3331879T3 (da) Benzazolforbindelser og fremgangsmåder til fremstilling og anvendelse af forbindelserne
DK3340973T3 (da) Farmaceutisk acceptable salte af beta-guanidinopropionsyre med forbedrede egenskaber og anvendelser deraf
DK3607065T3 (da) Fremgangsmåde og kit til konstruktion af nukleinsyrebibliotek
DK3190569T3 (da) Adgangsstyringssystem til anvendelse i områder med adgangsbegrænsning og industrielle miljøer
DK3100050T3 (da) Immobiliserede proteiner og anvendelse deraf
DK3201317T3 (da) Biomarkører for rheumatoid arthritis og anvendelse deraf
DK3356790T3 (da) System og fremgangsmåde til den optiske måling af stabiliteten og aggregeringen af partikler
DK2958573T3 (da) Farmaceutiske formuleringer af nitrit og anvendelser deraf
DK3297651T3 (da) Synergistisk kombination af neuronale levedygtighedsfaktorer og anvendelser deraf
DK3102613T3 (da) Polymerpartikler og anvendelser deraf
DK3209677T3 (da) Varianter af gal2-transporter og anvendelser deraf
DK3245219T3 (da) Glypicanepitoper og anvendelser deraf
DK3645717T3 (da) Modulære nukleinsyreadaptere
DK3321279T3 (da) Exenatidmodificeringsmiddel og anvendelse deraf