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DK3207134T3 - Kontiguitetsbevarende transposition - Google Patents

Kontiguitetsbevarende transposition Download PDF

Info

Publication number
DK3207134T3
DK3207134T3 DK15797490.8T DK15797490T DK3207134T3 DK 3207134 T3 DK3207134 T3 DK 3207134T3 DK 15797490 T DK15797490 T DK 15797490T DK 3207134 T3 DK3207134 T3 DK 3207134T3
Authority
DK
Denmark
Prior art keywords
contiguity
conversing
transposition
conversing transposition
contiguity conversing
Prior art date
Application number
DK15797490.8T
Other languages
English (en)
Inventor
Frank J Steemers
Fan Zhang
Jason Richard Betley
Niall Anthony Gormley
Wouter Meuleman
Jacqueline Weir
Avgousta Ioannou
Gareth Jenkins
Rosamond Jackson
Natalie Morrell
Dmitry K Pokholok
Steven J Norberg
Amirali Kia
Igor Goryshin
Rigo Pantoja
Kevin L Gunderson
Molly He
Original Assignee
Illumina Cambridge Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Cambridge Ltd filed Critical Illumina Cambridge Ltd
Priority claimed from PCT/US2015/056040 external-priority patent/WO2016061517A2/en
Application granted granted Critical
Publication of DK3207134T3 publication Critical patent/DK3207134T3/da

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1065Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1093General methods of preparing gene libraries, not provided for in other subgroups

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DK15797490.8T 2014-10-17 2015-10-16 Kontiguitetsbevarende transposition DK3207134T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462065544P 2014-10-17 2014-10-17
US201562157396P 2015-05-05 2015-05-05
PCT/US2015/056040 WO2016061517A2 (en) 2014-10-17 2015-10-16 Contiguity preserving transposition

Publications (1)

Publication Number Publication Date
DK3207134T3 true DK3207134T3 (da) 2019-09-23

Family

ID=54601997

Family Applications (1)

Application Number Title Priority Date Filing Date
DK15797490.8T DK3207134T3 (da) 2014-10-17 2015-10-16 Kontiguitetsbevarende transposition

Country Status (6)

Country Link
EP (2) EP3207134B1 (da)
CN (2) CN107969137B (da)
DK (1) DK3207134T3 (da)
ES (1) ES2745814T3 (da)
LT (1) LT3207134T (da)
PT (1) PT3207134T (da)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108728477B (zh) * 2017-04-24 2022-02-22 华东理工大学 一种高效的转座突变系统及构建方法
AU2019249422A1 (en) 2018-04-02 2020-10-15 Grail, Llc Methylation markers and targeted methylation probe panels
CA3097976A1 (en) * 2018-05-03 2019-11-07 Becton, Dickinson And Company High throughput multiomics sample analysis
EP3856903A4 (en) 2018-09-27 2022-07-27 Grail, LLC METHYLATION MARKER AND TARGETED METHYLATION PROBE PANEL
EP3859014A4 (en) * 2018-09-27 2022-04-27 BGI Shenzhen METHOD OF CONSTRUCTING SEQUENCING LIBRARY, OBTAINED SEQUENCING LIBRARY AND METHOD OF SEQUENCING
DK3887540T3 (da) * 2018-11-30 2023-09-18 Illumina Inc Analyse af multiple analytter under anvendelse af et enkelt assay
SG11202105836XA (en) * 2019-07-12 2021-07-29 Illumina Cambridge Ltd Compositions and methods for preparing nucleic acid sequencing libraries using crispr/cas9 immobilized on a solid support
SG11202109768PA (en) * 2019-12-02 2021-10-28 Illumina Cambridge Ltd Time-based cluster imaging of amplified contiguity preserved liarary fragments of genomic dna
IL294909A (en) 2020-02-13 2022-09-01 Zymergen Inc A metagenomic library and natural product discovery platform
US20230174969A1 (en) * 2020-05-18 2023-06-08 Mgi Tech Co., Ltd. Barcoded transposase complex and application thereof in high-throughput sequencing
CA3198842A1 (en) * 2020-10-21 2022-04-28 Illumina, Inc. Sequencing templates comprising multiple inserts and compositions and methods for improving sequencing throughput
CN112795563A (zh) * 2021-03-23 2021-05-14 上海欣百诺生物科技有限公司 生物素化的转座体在回收CUT&Tag或ATAC-seq产物中的用途及方法
WO2023225519A1 (en) 2022-05-17 2023-11-23 10X Genomics, Inc. Modified transposons, compositions and uses thereof
CN115747301B (zh) * 2022-08-01 2023-12-22 深圳赛陆医疗科技有限公司 一种测序文库的构建方法、构建测序文库的试剂盒及基因测序方法

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5677170A (en) 1994-03-02 1997-10-14 The Johns Hopkins University In vitro transposition of artificial transposons
CN101432439B (zh) * 2006-02-24 2013-07-24 考利达基因组股份有限公司 Dna阵列上的高通量基因组测序
JP5794572B2 (ja) * 2008-10-24 2015-10-14 エピセンター テクノロジーズ コーポレイションEpicentre Technologies Corporation 核酸を修飾するためのトランスポゾン末端組成物及び方法
US9029103B2 (en) 2010-08-27 2015-05-12 Illumina Cambridge Limited Methods for sequencing polynucleotides
WO2012027572A2 (en) * 2010-08-27 2012-03-01 Genentech, Inc. Methods for nucleic acid capture and sequencing
ES2966040T3 (es) * 2010-10-08 2024-04-18 Harvard College Alto rendimiento de células individuales con código de barra
US8829171B2 (en) * 2011-02-10 2014-09-09 Illumina, Inc. Linking sequence reads using paired code tags
AU2011323107B2 (en) * 2010-11-05 2015-09-10 Illumina, Inc. Linking sequence reads using paired code tags
DK2668273T3 (da) * 2011-01-28 2016-04-18 Illumina Inc Oligonukleotiderstatning for di-taggede og retningsbiblioteker
EP2670894B1 (en) * 2011-02-02 2017-11-29 University Of Washington Through Its Center For Commercialization Massively parallel continguity mapping
WO2012108864A1 (en) 2011-02-08 2012-08-16 Illumina, Inc. Selective enrichment of nucleic acids
NO2694769T3 (da) * 2012-03-06 2018-03-03
LT2825672T (lt) 2012-03-13 2019-06-10 Swift Biosciences, Inc. Būdai ir kompozicijos, skirti valdomam pagal dydį substrato polinukleotidų homopolimeriniam uodegų formavimui su nukleorūgščių polimeraze
US9644199B2 (en) * 2012-10-01 2017-05-09 Agilent Technologies, Inc. Immobilized transposase complexes for DNA fragmentation and tagging
US9683230B2 (en) * 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
CN105074010B (zh) * 2013-03-07 2018-04-17 积水医疗株式会社 甲基化dna 的检测方法
EP3553175B1 (en) * 2013-03-13 2021-07-14 Illumina, Inc. Method of preparing a nucleic acid sequencing library
KR102536833B1 (ko) * 2013-08-28 2023-05-26 벡톤 디킨슨 앤드 컴퍼니 대량의 동시 단일 세포 분석
EP3089822B1 (en) * 2013-12-30 2022-04-06 Atreca, Inc. Analysis of nucleic acids associated with single cells using nucleic acid barcodes

Also Published As

Publication number Publication date
EP3207134A2 (en) 2017-08-23
CN107969137B (zh) 2021-10-26
ES2745814T3 (es) 2020-03-03
EP3636757A1 (en) 2020-04-15
LT3207134T (lt) 2019-09-10
EP3207134B1 (en) 2019-07-03
CN114045282A (zh) 2022-02-15
CN107969137A (zh) 2018-04-27
PT3207134T (pt) 2019-09-17

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