DK0530447T3 - Method for purifying IgG monoclonal antibodies - Google Patents
Method for purifying IgG monoclonal antibodiesInfo
- Publication number
- DK0530447T3 DK0530447T3 DK92108912.4T DK92108912T DK0530447T3 DK 0530447 T3 DK0530447 T3 DK 0530447T3 DK 92108912 T DK92108912 T DK 92108912T DK 0530447 T3 DK0530447 T3 DK 0530447T3
- Authority
- DK
- Denmark
- Prior art keywords
- monoclonal antibodies
- igg monoclonal
- purifying igg
- purifying
- immunotherapy
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
- C07K16/065—Purification, fragmentation
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
The present invention relates to a process for purifying IgG monoclonal antibodies by a combination of three chromatography steps, namely on an anion exchanger, on a cation exchanger and on a hydrophobic phase with a specific sterilisation step. This results in an antibody solution with a purity of at least 99.5%, which can thus be used intravenously in immunotherapy.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE4118912A DE4118912C1 (en) | 1991-06-08 | 1991-06-08 |
Publications (1)
Publication Number | Publication Date |
---|---|
DK0530447T3 true DK0530447T3 (en) | 1998-01-12 |
Family
ID=6433511
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK92108912.4T DK0530447T3 (en) | 1991-06-08 | 1992-05-27 | Method for purifying IgG monoclonal antibodies |
Country Status (7)
Country | Link |
---|---|
EP (1) | EP0530447B1 (en) |
JP (1) | JP2644946B2 (en) |
AT (1) | ATE159532T1 (en) |
DE (2) | DE4118912C1 (en) |
DK (1) | DK0530447T3 (en) |
ES (1) | ES2108059T3 (en) |
GR (1) | GR3025603T3 (en) |
Families Citing this family (22)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2706466B1 (en) * | 1993-06-14 | 1995-08-25 | Aetsrn | Immunoglobulin G concentrate for therapeutic use and process for producing said concentrate. |
IL121900A (en) * | 1997-10-07 | 2001-12-23 | Omrix Biopharmaceuticals Ltd | Method for the purification of immunoglobulins |
SK288128B6 (en) * | 1998-06-09 | 2013-10-02 | Csl Behring Ag | Process for purifying immunoglobulin G (IgG), liquid immunoglobulin product and use thereof for the preparation of a medicament |
DK2270044T3 (en) | 1998-06-09 | 2015-01-26 | Csl Behring Ag | Liquid immunoglobulin G (IgG) product |
SE0001128D0 (en) * | 2000-03-30 | 2000-03-30 | Amersham Pharm Biotech Ab | A method of producing IgG |
JPWO2004087761A1 (en) * | 2003-03-31 | 2006-07-27 | 麒麟麦酒株式会社 | Purification of human monoclonal antibodies and human polyclonal antibodies |
EP1532983A1 (en) | 2003-11-18 | 2005-05-25 | ZLB Bioplasma AG | Immunoglobulin preparations having increased stability |
TWI320788B (en) | 2005-05-25 | 2010-02-21 | Hoffmann La Roche | Method for the purification of antibodies |
WO2008145351A1 (en) | 2007-06-01 | 2008-12-04 | F. Hoffmann-La Roche Ag | Immunoglobulin purification |
CL2008002054A1 (en) | 2007-07-17 | 2009-05-29 | Hoffmann La Roche | Method for the regeneration of a cation exchange chromatography column after elusion of monopeglated erythropoietin and method to obtain a monopeglated erythropoietin, incorporating the regeneration method of the cation exchange column. |
AR067537A1 (en) | 2007-07-17 | 2009-10-14 | Hoffmann La Roche | PURIFIED POLYPEPTIDES PURIFICATION |
WO2009058769A1 (en) * | 2007-10-30 | 2009-05-07 | Schering Corporation | Purification of antibodies containing hydrophobic variants |
MX2011006654A (en) | 2008-12-22 | 2011-07-12 | Hoffmann La Roche | Immunoglobulin purification. |
CN102753197B (en) | 2010-02-04 | 2014-05-14 | 瑞士杰特贝林生物制品有限公司 | Immunoglobulin preparation |
MX2012009283A (en) * | 2010-02-12 | 2012-09-07 | Dsm Ip Assets Bv | Single unit antibody purification. |
EP2361636A1 (en) | 2010-02-26 | 2011-08-31 | CSL Behring AG | Immunoglobulin preparation and storage system for an immunoglobulin preparation |
JP5735650B2 (en) | 2010-09-14 | 2015-06-17 | エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft | Method for purifying PEGylated erythropoietin |
JP2013540787A (en) * | 2010-11-01 | 2013-11-07 | ディーエスエム アイピー アセッツ ビー.ブイ. | Single unit ion exchange chromatography antibody purification |
CA2838814A1 (en) | 2011-08-25 | 2013-02-28 | F.Hoffmann-La Roche Ag | Cation and anion exchange chromatography method |
EP2596812B1 (en) | 2011-11-22 | 2015-06-10 | Heraeus Medical GmbH | Sterilisation of polymerisable monomers |
EP3118209B1 (en) * | 2014-03-11 | 2020-02-19 | Green Cross Holdings Corporation | Method for purifying immunoglobulin |
KR102470282B1 (en) | 2016-07-15 | 2022-11-24 | 에프. 호프만-라 로슈 아게 | Method for purifying PEGized erythropoietin |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4469630A (en) * | 1983-11-25 | 1984-09-04 | J. T. Baker Chemical Co. | Purification of monoclonal antibodies |
US4743680A (en) * | 1985-02-01 | 1988-05-10 | New York University | Method for purifying antihemophilic factor |
DE3640513A1 (en) * | 1986-11-27 | 1988-06-09 | Biotest Pharma Gmbh | METHOD FOR THE PRODUCTION OF A VIRUS-SAFE, STORAGE-STABLE AND INTRAVENOES COMPATIBLE IMMUNGLOBULIN-G-PRAEPARATES |
DE3641115A1 (en) * | 1986-12-02 | 1988-06-16 | Lentia Gmbh | METHOD FOR PRODUCING AN INTRAVENOUS APPLICABLE AND STABLE IN LIQUID FORM IMMUNOGLOBULINS |
CA1323567C (en) * | 1987-10-05 | 1993-10-26 | Gene R. Nathans | Method for purification of antibodies |
US5118796A (en) * | 1987-12-09 | 1992-06-02 | Centocor, Incorporated | Efficient large-scale purification of immunoglobulins and derivatives |
CA2005600A1 (en) * | 1988-12-20 | 1990-06-20 | Cal-Henrik Heldin | Endothelial cell growth factor |
-
1991
- 1991-06-08 DE DE4118912A patent/DE4118912C1/de not_active Expired - Fee Related
-
1992
- 1992-05-27 DE DE59208982T patent/DE59208982D1/en not_active Expired - Lifetime
- 1992-05-27 DK DK92108912.4T patent/DK0530447T3/en active
- 1992-05-27 ES ES92108912T patent/ES2108059T3/en not_active Expired - Lifetime
- 1992-05-27 AT AT92108912T patent/ATE159532T1/en active
- 1992-05-27 EP EP92108912A patent/EP0530447B1/en not_active Expired - Lifetime
- 1992-06-05 JP JP4145807A patent/JP2644946B2/en not_active Expired - Fee Related
-
1997
- 1997-12-05 GR GR970403248T patent/GR3025603T3/en unknown
Also Published As
Publication number | Publication date |
---|---|
DE4118912C1 (en) | 1992-07-02 |
GR3025603T3 (en) | 1998-03-31 |
DE59208982D1 (en) | 1997-11-27 |
ATE159532T1 (en) | 1997-11-15 |
EP0530447A3 (en) | 1993-08-18 |
EP0530447A2 (en) | 1993-03-10 |
EP0530447B1 (en) | 1997-10-22 |
JP2644946B2 (en) | 1997-08-25 |
ES2108059T3 (en) | 1997-12-16 |
JPH07267997A (en) | 1995-10-17 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
DK0530447T3 (en) | Method for purifying IgG monoclonal antibodies | |
DK0821695T3 (en) | Purification of antibody by hydrophobic interaction chromatography at low pH | |
WO1990010709A3 (en) | Monoclonal antibodies for metallic cations on small molecules | |
CA2121257A1 (en) | Stabilised Antibodies | |
DK0538754T3 (en) | Use of antibody-containing preparations for immunosuppression | |
NZ509135A (en) | A third generation intravenous immunoglobulin G (IVIG) purified using column chromatographic ion exchange methods | |
BR9804826A (en) | Antigenic fusion protein carrying galoc epitopes 1.3 gal | |
MX9603637A (en) | Antibody purification. | |
AU8142982A (en) | A process and apparatus for the recovery of immunoglobulins | |
Saluk et al. | The unique molecular weight of the heavy chain from human IgG3 | |
EP0282317A3 (en) | Purified platelet-derived growth factor and methods for purification thereof | |
DK407987D0 (en) | APOLIPOPROTEIN B-SPECIFIC MONOCLONAL ANTIBODIES AND HYBRIDOMES PRODUCING THESE ANTIBODIES | |
GB8910263D0 (en) | Monoclonal antibodies specific for an immunoglobulin isotype | |
AU5406800A (en) | Method for carrying out the chromatographic fractionation of plasma or serum, preparations obtained thereby and their use | |
AU2707992A (en) | Monoclonal antibodies to hepatitis c virus | |
DK0597358T3 (en) | Process for Quantitative Purification of Glycolipids | |
ITTO920592A1 (en) | PROCEDURE FOR THE PRODUCTION OF ITTRIO-90 AND ITTRIO-90 GENERATOR | |
FI860937A (en) | Method and reagent for the determination of follicle-stimulating hormone and suitable monoclonal antibodies | |
DK0513565T3 (en) | Monoclonal antibodies against the plasmin-antiplasmin complex, method for their preparation and their use | |
EP0539584A4 (en) | ||
IT1237997B (en) | THIOPHILIC ABSORBENTS | |
ATE193021T1 (en) | LIPOPROTEIN (A) PEPTIDES AND THEIR USE |