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DK0530447T3 - Method for purifying IgG monoclonal antibodies - Google Patents

Method for purifying IgG monoclonal antibodies

Info

Publication number
DK0530447T3
DK0530447T3 DK92108912.4T DK92108912T DK0530447T3 DK 0530447 T3 DK0530447 T3 DK 0530447T3 DK 92108912 T DK92108912 T DK 92108912T DK 0530447 T3 DK0530447 T3 DK 0530447T3
Authority
DK
Denmark
Prior art keywords
monoclonal antibodies
igg monoclonal
purifying igg
purifying
immunotherapy
Prior art date
Application number
DK92108912.4T
Other languages
Danish (da)
Inventor
Norbert Dr Kothe
Dieter Dr Rudnick
Ulf Dr Bethke
Michael Dr Kloft
Detlef Dr Rohm
Original Assignee
Biotest Pharma Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biotest Pharma Gmbh filed Critical Biotest Pharma Gmbh
Application granted granted Critical
Publication of DK0530447T3 publication Critical patent/DK0530447T3/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065Purification, fragmentation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

The present invention relates to a process for purifying IgG monoclonal antibodies by a combination of three chromatography steps, namely on an anion exchanger, on a cation exchanger and on a hydrophobic phase with a specific sterilisation step. This results in an antibody solution with a purity of at least 99.5%, which can thus be used intravenously in immunotherapy.
DK92108912.4T 1991-06-08 1992-05-27 Method for purifying IgG monoclonal antibodies DK0530447T3 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE4118912A DE4118912C1 (en) 1991-06-08 1991-06-08

Publications (1)

Publication Number Publication Date
DK0530447T3 true DK0530447T3 (en) 1998-01-12

Family

ID=6433511

Family Applications (1)

Application Number Title Priority Date Filing Date
DK92108912.4T DK0530447T3 (en) 1991-06-08 1992-05-27 Method for purifying IgG monoclonal antibodies

Country Status (7)

Country Link
EP (1) EP0530447B1 (en)
JP (1) JP2644946B2 (en)
AT (1) ATE159532T1 (en)
DE (2) DE4118912C1 (en)
DK (1) DK0530447T3 (en)
ES (1) ES2108059T3 (en)
GR (1) GR3025603T3 (en)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2706466B1 (en) * 1993-06-14 1995-08-25 Aetsrn Immunoglobulin G concentrate for therapeutic use and process for producing said concentrate.
IL121900A (en) * 1997-10-07 2001-12-23 Omrix Biopharmaceuticals Ltd Method for the purification of immunoglobulins
SK288128B6 (en) * 1998-06-09 2013-10-02 Csl Behring Ag Process for purifying immunoglobulin G (IgG), liquid immunoglobulin product and use thereof for the preparation of a medicament
DK2270044T3 (en) 1998-06-09 2015-01-26 Csl Behring Ag Liquid immunoglobulin G (IgG) product
SE0001128D0 (en) * 2000-03-30 2000-03-30 Amersham Pharm Biotech Ab A method of producing IgG
JPWO2004087761A1 (en) * 2003-03-31 2006-07-27 麒麟麦酒株式会社 Purification of human monoclonal antibodies and human polyclonal antibodies
EP1532983A1 (en) 2003-11-18 2005-05-25 ZLB Bioplasma AG Immunoglobulin preparations having increased stability
TWI320788B (en) 2005-05-25 2010-02-21 Hoffmann La Roche Method for the purification of antibodies
WO2008145351A1 (en) 2007-06-01 2008-12-04 F. Hoffmann-La Roche Ag Immunoglobulin purification
CL2008002054A1 (en) 2007-07-17 2009-05-29 Hoffmann La Roche Method for the regeneration of a cation exchange chromatography column after elusion of monopeglated erythropoietin and method to obtain a monopeglated erythropoietin, incorporating the regeneration method of the cation exchange column.
AR067537A1 (en) 2007-07-17 2009-10-14 Hoffmann La Roche PURIFIED POLYPEPTIDES PURIFICATION
WO2009058769A1 (en) * 2007-10-30 2009-05-07 Schering Corporation Purification of antibodies containing hydrophobic variants
MX2011006654A (en) 2008-12-22 2011-07-12 Hoffmann La Roche Immunoglobulin purification.
CN102753197B (en) 2010-02-04 2014-05-14 瑞士杰特贝林生物制品有限公司 Immunoglobulin preparation
MX2012009283A (en) * 2010-02-12 2012-09-07 Dsm Ip Assets Bv Single unit antibody purification.
EP2361636A1 (en) 2010-02-26 2011-08-31 CSL Behring AG Immunoglobulin preparation and storage system for an immunoglobulin preparation
JP5735650B2 (en) 2010-09-14 2015-06-17 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft Method for purifying PEGylated erythropoietin
JP2013540787A (en) * 2010-11-01 2013-11-07 ディーエスエム アイピー アセッツ ビー.ブイ. Single unit ion exchange chromatography antibody purification
CA2838814A1 (en) 2011-08-25 2013-02-28 F.Hoffmann-La Roche Ag Cation and anion exchange chromatography method
EP2596812B1 (en) 2011-11-22 2015-06-10 Heraeus Medical GmbH Sterilisation of polymerisable monomers
EP3118209B1 (en) * 2014-03-11 2020-02-19 Green Cross Holdings Corporation Method for purifying immunoglobulin
KR102470282B1 (en) 2016-07-15 2022-11-24 에프. 호프만-라 로슈 아게 Method for purifying PEGized erythropoietin

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4469630A (en) * 1983-11-25 1984-09-04 J. T. Baker Chemical Co. Purification of monoclonal antibodies
US4743680A (en) * 1985-02-01 1988-05-10 New York University Method for purifying antihemophilic factor
DE3640513A1 (en) * 1986-11-27 1988-06-09 Biotest Pharma Gmbh METHOD FOR THE PRODUCTION OF A VIRUS-SAFE, STORAGE-STABLE AND INTRAVENOES COMPATIBLE IMMUNGLOBULIN-G-PRAEPARATES
DE3641115A1 (en) * 1986-12-02 1988-06-16 Lentia Gmbh METHOD FOR PRODUCING AN INTRAVENOUS APPLICABLE AND STABLE IN LIQUID FORM IMMUNOGLOBULINS
CA1323567C (en) * 1987-10-05 1993-10-26 Gene R. Nathans Method for purification of antibodies
US5118796A (en) * 1987-12-09 1992-06-02 Centocor, Incorporated Efficient large-scale purification of immunoglobulins and derivatives
CA2005600A1 (en) * 1988-12-20 1990-06-20 Cal-Henrik Heldin Endothelial cell growth factor

Also Published As

Publication number Publication date
DE4118912C1 (en) 1992-07-02
GR3025603T3 (en) 1998-03-31
DE59208982D1 (en) 1997-11-27
ATE159532T1 (en) 1997-11-15
EP0530447A3 (en) 1993-08-18
EP0530447A2 (en) 1993-03-10
EP0530447B1 (en) 1997-10-22
JP2644946B2 (en) 1997-08-25
ES2108059T3 (en) 1997-12-16
JPH07267997A (en) 1995-10-17

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