[go: up one dir, main page]
More Web Proxy on the site http://driver.im/

DK0388593T3 - Method for Expression of Polygalacturonidase and Pectin Sterase in Aspergillus niger and Aspergillus awamori - Google Patents

Method for Expression of Polygalacturonidase and Pectin Sterase in Aspergillus niger and Aspergillus awamori

Info

Publication number
DK0388593T3
DK0388593T3 DK90101399.5T DK90101399T DK0388593T3 DK 0388593 T3 DK0388593 T3 DK 0388593T3 DK 90101399 T DK90101399 T DK 90101399T DK 0388593 T3 DK0388593 T3 DK 0388593T3
Authority
DK
Denmark
Prior art keywords
aspergillus
polygalacturonidase
sterase
pectin
expression
Prior art date
Application number
DK90101399.5T
Other languages
Danish (da)
Inventor
Edeltraud Dr Rer Na Ruttkowksi
Quoc Khahn Dr Dipl-Ing Nguyen
Michael Dr Dipl Bio Gottschalk
Klaus-Dieter Prof Dr Rer Jany
Fridolin Dr Dipl-Chem Loeffler
Wolfgang Prof Dr R Piepersberg
Erwin Dr Schuster
Hans G Gassen
Original Assignee
Roehm Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roehm Gmbh filed Critical Roehm Gmbh
Application granted granted Critical
Publication of DK0388593T3 publication Critical patent/DK0388593T3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01067—Galacturan 1,4-alpha-galacturonidase (3.2.1.67)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01011—Pectinesterase (3.1.1.11)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

A process for the preparation of a recombinant Asp.niger or Asp.awamori which is transformed with a polygalacturonidase or pectinesterase gene which is derived from Asp.niger by cloning the appropriate recombinant vector and transformation into Asp.niger or Asp.awamori is disclosed.
DK90101399.5T 1989-03-17 1990-01-24 Method for Expression of Polygalacturonidase and Pectin Sterase in Aspergillus niger and Aspergillus awamori DK0388593T3 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE3908813A DE3908813A1 (en) 1989-03-17 1989-03-17 METHOD FOR EXPRESSING A GENES FROM ASPERGILLUS NIGER IN AN ASPERGILLUS

Publications (1)

Publication Number Publication Date
DK0388593T3 true DK0388593T3 (en) 1996-05-28

Family

ID=6376595

Family Applications (1)

Application Number Title Priority Date Filing Date
DK90101399.5T DK0388593T3 (en) 1989-03-17 1990-01-24 Method for Expression of Polygalacturonidase and Pectin Sterase in Aspergillus niger and Aspergillus awamori

Country Status (6)

Country Link
EP (1) EP0388593B1 (en)
JP (1) JPH02283287A (en)
AT (1) ATE136587T1 (en)
DE (2) DE3908813A1 (en)
DK (1) DK0388593T3 (en)
ES (1) ES2087092T3 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0421919A3 (en) * 1989-09-02 1992-04-15 Ciba-Geigy Ag Polygalacturonase encoding fungal expression system
DK42092D0 (en) * 1992-03-27 1992-03-27 Novo Nordisk As
WO1994014966A1 (en) * 1992-12-24 1994-07-07 Gist-Brocades N.V. CLONING AND EXPRESSION OF THE EXO-POLYGALACTURONASE GENE FROM $i(ASPERGILLUS)
US5707847A (en) * 1993-04-30 1998-01-13 Novo Nordisk A/S Enzyme exhibiting pectin methylesterase activity
AU710705B2 (en) 1995-09-22 1999-09-30 Novozymes A/S Method for reducing the serum separation in a pectin containing aqueous mass
AU1719497A (en) * 1996-02-21 1997-09-10 Novo Nordisk A/S An enzyme with pectin esterase activity
US7303917B2 (en) 1998-03-20 2007-12-04 Her Majesty The Queen In Right Of Canada As Represented By The Minister Of Agriculture And Agri-Food Eastern Cereal & Oilseed, Research Center Modification of pollen coat protein composition
US6602696B1 (en) 1998-09-18 2003-08-05 Dsm N. V. Aspergillus tubigensis polygalacturonase
DE102008024778A1 (en) 2008-05-23 2009-11-26 Ab Enzymes Gmbh Use of pectinolytic enzymes for the treatment of fruit and vegetable mash and enzyme sequences thereto
WO2010070146A1 (en) 2008-12-19 2010-06-24 Danisco A/S Process for production of an enzyme product
WO2014028772A2 (en) 2012-08-16 2014-02-20 Bangladesh Jute Research Institute Pectin degrading enzymes from macrophomina phaseolina and uses thereof
CN107446903B (en) * 2017-09-13 2020-06-23 云南师范大学 Salt-tolerant ethanol-tolerant pectinase with 3 optimal pH values and gene thereof

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE1805808C3 (en) * 1968-10-29 1979-06-21 Roehm Gmbh, 6100 Darmstadt Macerating fruit or vegetable pulp
DE3044455C1 (en) * 1980-11-26 1982-07-08 Röhm GmbH, 6100 Darmstadt Production of pectin esterase
CA1295563C (en) * 1985-11-01 1992-02-11 Robert T. Garvin Production of active proteins containing cystine residues
GB8626879D0 (en) * 1986-11-11 1986-12-10 Ici Plc Dna
DE3801005A1 (en) * 1988-01-15 1989-07-27 Roehm Gmbh METHOD FOR PRODUCING A MACERATING ASPERGILLUS ENZYME

Also Published As

Publication number Publication date
EP0388593B1 (en) 1996-04-10
ATE136587T1 (en) 1996-04-15
ES2087092T3 (en) 1996-07-16
JPH02283287A (en) 1990-11-20
DE3908813A1 (en) 1990-09-20
EP0388593A1 (en) 1990-09-26
DE59010266D1 (en) 1996-05-15

Similar Documents

Publication Publication Date Title
DK0388593T3 (en) Method for Expression of Polygalacturonidase and Pectin Sterase in Aspergillus niger and Aspergillus awamori
ATE174900T1 (en) METHOD FOR PRODUCING MALEINAMIC ACID
DK219087D0 (en) VECTOR SYSTEM FOR USING TRANSFORMATION OF TRICHODERMA, TRANSFORMED TRICHODERMAS STAMES AND PROCEDURES FOR PRODUCING PROTEINS HEREIN
DE58907174D1 (en) Process for the production of molten pig iron and plant for carrying out the process.
HU200794B (en) Process for expressing malaria antigenes
IS3865A (en) An analogous method for the production of benzofuran derivatives and intermediates for use in the process
DE3855449D1 (en) DNA clones from human thrombomodulin
IT8419740A0 (en) DERIVATIVES OF BENZOQUINOLYZIN CARBOXYLIC ACID, AND PROCESS FOR THE PREPARATION OF THEM.
EP0562508A3 (en) Protein with bone formation ability and process for its production
DK0566645T3 (en) Process for preparing a vegetable protein hydrolyzate
EP0307472A4 (en) Immunogenic recombinant yeast expression product and method for purifying it.
IS4033A (en) Similar method for the production of isobenzofuranyl derivatives and intermediates for use with the method
EP0245218A3 (en) A plasmid vector for expression in bacillus and used for cloning the structural gene which codes for the human growth hormone and a method of producing the hormone
ATE196927T1 (en) METHOD FOR PRODUCING PROTEASE INHIBITORS
ES554327A0 (en) A PROCEDURE FOR PREPARING A RECOMBINANT PLASMID
EP0303714A4 (en) Method of generating free positronium radiant light and apparatus therefor.
DE3870943D1 (en) METHOD FOR PRODUCING 7-CHLORINE-CHINOLIN-8-CARBONIC ACIDS.
DE3574731D1 (en) A HUMAN T-CELL GROWTH FACTOR.
DK0533610T3 (en) Plant and process for producing cyanuric acid
DE3867474D1 (en) METHOD FOR PRODUCING CHLORORHODIUM ACID.
ATE169964T1 (en) PROCESS USING FOAM SLAG IN THE PRODUCTION OF STAINLESS STEEL IN ELECTRIC ARC FURNACE
ITMI931273A0 (en) PROCEDURE FOR THE PRODUCTION OF AN ALIPHATIC CARBOXYLIC ACID - FUNCTIONALIZED AND INTERMEDIATES OF SUCH PROCESS
DE3584528D1 (en) CATALYTIC METHOD FOR PRODUCING AMIDES.
ATE117289T1 (en) PRODUCTION OF PYRIDAZINONE.
DK281385A (en) METHOD OF PREPARING HYDROQUINON MONONCARBOXYLATES