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DE69434083D1 - Expression eines fusionspolypeptides, das ohne leadersequenz aus dem cytoplasma transportiert wird - Google Patents

Expression eines fusionspolypeptides, das ohne leadersequenz aus dem cytoplasma transportiert wird

Info

Publication number
DE69434083D1
DE69434083D1 DE69434083T DE69434083T DE69434083D1 DE 69434083 D1 DE69434083 D1 DE 69434083D1 DE 69434083 T DE69434083 T DE 69434083T DE 69434083 T DE69434083 T DE 69434083T DE 69434083 D1 DE69434083 D1 DE 69434083D1
Authority
DE
Germany
Prior art keywords
polypeptide
fusion
expression
leader sequence
polypeptides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69434083T
Other languages
English (en)
Other versions
DE69434083T2 (de
Inventor
Desmond Mascarenhas
Yang Zhang
Pamela S Olson
David R Olsen
Pedro A Carrillo
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Celtrix Pharmaceuticals Inc
Original Assignee
Celtrix Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Celtrix Pharmaceuticals Inc filed Critical Celtrix Pharmaceuticals Inc
Publication of DE69434083D1 publication Critical patent/DE69434083D1/de
Application granted granted Critical
Publication of DE69434083T2 publication Critical patent/DE69434083T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/545—IL-1
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/71—Receptors; Cell surface antigens; Cell surface determinants for growth factors; for growth regulators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/32—Fusion polypeptide fusions with soluble part of a cell surface receptor, "decoy receptors"
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/35—Fusion polypeptide containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/95—Fusion polypeptide containing a motif/fusion for degradation (ubiquitin fusions, PEST sequence)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DE69434083T 1993-08-02 1994-08-02 Expression eines fusionspolypeptides, das ohne leadersequenz aus dem cytoplasma transportiert wird Expired - Fee Related DE69434083T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US100744 1993-08-02
US08/100,744 US5563046A (en) 1993-08-02 1993-08-02 Fusion polypeptides and proteins
PCT/US1994/008776 WO1995004076A1 (en) 1993-08-02 1994-08-02 Expression of fusion polypeptides transported out of the cytoplasm without leader sequences

Publications (2)

Publication Number Publication Date
DE69434083D1 true DE69434083D1 (de) 2004-11-25
DE69434083T2 DE69434083T2 (de) 2006-02-09

Family

ID=22281315

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69434083T Expired - Fee Related DE69434083T2 (de) 1993-08-02 1994-08-02 Expression eines fusionspolypeptides, das ohne leadersequenz aus dem cytoplasma transportiert wird

Country Status (8)

Country Link
US (3) US5563046A (de)
EP (1) EP0722461B1 (de)
JP (1) JP3370094B2 (de)
AT (1) ATE280234T1 (de)
AU (1) AU688363B2 (de)
CA (1) CA2168429C (de)
DE (1) DE69434083T2 (de)
WO (1) WO1995004076A1 (de)

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US6391585B1 (en) * 1997-05-27 2002-05-21 Hanil Synthetic Fiber Co., Ltd. Process for preparing recombinant proteins using highly efficient expression vector from saccharomyces cerevisiae
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US20050033033A1 (en) * 1998-05-04 2005-02-10 Heinz Kohler Trans-membrane-antibody induced inhibition of apoptosis
US20030103984A1 (en) * 1998-05-04 2003-06-05 Heinz Kohler Fusion proteins of biologically active peptides and antibodies
US20040185039A1 (en) * 2002-08-30 2004-09-23 Heinz Kohler Therapeutic applications of noncovalent dimerizing antibodies
US6077689A (en) * 1997-12-24 2000-06-20 Amgen Inc. Enhanced solubility of recombinant proteins
US6008329A (en) * 1998-03-06 1999-12-28 The United States Of America As Represented By The Secretary Of The Army Method for purifying cholera toxin
US20090208418A1 (en) * 2005-04-29 2009-08-20 Innexus Biotechnology Internaltional Ltd. Superantibody synthesis and use in detection, prevention and treatment of disease
US6417330B1 (en) 1998-06-01 2002-07-09 Celtrix Pharmaceuticals, Inc. Insulin-like growth factor binding protein variants
US6436897B2 (en) 1998-06-01 2002-08-20 Celtrix Pharmaceuticals, Inc. Pharmaceutical formulations for IGF/IGFBP
US7288516B1 (en) 1999-09-20 2007-10-30 Celtrix Pharmaceuticals, Inc. Null IGF for the treatment of cancer
US6040292A (en) * 1999-06-04 2000-03-21 Celtrix Pharmaceuticals, Inc. Methods for treating diabetes
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US7538082B2 (en) * 2001-04-24 2009-05-26 The General Hospital Corporation Methods and compositions for treating oral and esophageal lesions
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US20030181384A1 (en) * 2001-09-06 2003-09-25 Podolsky Daniel K. Methods and compositions for treating vaginal, cervical, and uterine epithelial lesions
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JP2005508937A (ja) * 2001-10-05 2005-04-07 ザ ジェネラル ホスピタル コーポレーション 皮膚病変を治療するための方法および組成物
CN1617739A (zh) * 2001-11-28 2005-05-18 综合医院公司 用于治疗呼吸道上皮病变的方法和组合物
US8535908B2 (en) * 2002-01-04 2013-09-17 Brookhaven Science Associates, Llc Facilitating protein solubility by use of peptide extensions
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AU2003224773B2 (en) * 2002-03-26 2010-08-26 The General Hospital Corporation Combination therapy using trefoil peptides
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US8227411B2 (en) 2002-08-20 2012-07-24 BioSurface Engineering Technologies, Incle FGF growth factor analogs
US7166574B2 (en) 2002-08-20 2007-01-23 Biosurface Engineering Technologies, Inc. Synthetic heparin-binding growth factor analogs
US7598224B2 (en) 2002-08-20 2009-10-06 Biosurface Engineering Technologies, Inc. Dual chain synthetic heparin-binding growth factor analogs
US7482427B2 (en) * 2002-08-20 2009-01-27 Biosurface Engineering Technologies, Inc. Positive modulator of bone morphogenic protein-2
US20060188471A1 (en) * 2002-10-31 2006-08-24 Podolsky Daniel K Methods of treating epithelial lesions
PL1646650T3 (pl) * 2003-06-13 2009-09-30 Insmed Inc Układy do ekspresji rekombinowanego IGF
EP2336153B1 (de) * 2003-11-21 2016-03-30 Pfenex Inc. Verbessertes Expressionssystem mit SEC-System-Sekretion
DK1713930T3 (da) 2004-01-17 2011-01-17 Korea Res Inst Of Bioscience Hurtig screeningsmetode for translationsfusionspartnere til fremstilling af rekombinante proteiner og translationsfusionspartnere screenet derfra
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Also Published As

Publication number Publication date
EP0722461B1 (de) 2004-10-20
CA2168429C (en) 2001-06-05
JP3370094B2 (ja) 2003-01-27
AU688363B2 (en) 1998-03-12
EP0722461A1 (de) 1996-07-24
AU7519494A (en) 1995-02-28
CA2168429A1 (en) 1995-02-09
US5563046A (en) 1996-10-08
US5629172A (en) 1997-05-13
EP0722461A4 (de) 1997-02-26
JPH09504166A (ja) 1997-04-28
WO1995004076A1 (en) 1995-02-09
DE69434083T2 (de) 2006-02-09
ATE280234T1 (de) 2004-11-15
US5830706A (en) 1998-11-03

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