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DE602006018054D1 - PICHIA PASTORIS TRANSLATION ELONGATION FACTOR PROMOTER AND METHOD FOR PRODUCING RECOMBINANT PROTEIN USING THEREOF - Google Patents

PICHIA PASTORIS TRANSLATION ELONGATION FACTOR PROMOTER AND METHOD FOR PRODUCING RECOMBINANT PROTEIN USING THEREOF

Info

Publication number
DE602006018054D1
DE602006018054D1 DE602006018054T DE602006018054T DE602006018054D1 DE 602006018054 D1 DE602006018054 D1 DE 602006018054D1 DE 602006018054 T DE602006018054 T DE 602006018054T DE 602006018054 T DE602006018054 T DE 602006018054T DE 602006018054 D1 DE602006018054 D1 DE 602006018054D1
Authority
DE
Germany
Prior art keywords
promoter
pichia pastoris
elongation factor
translation elongation
recombinant protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
DE602006018054T
Other languages
German (de)
Inventor
Hong Weon Lee
Jung Oh Ahn
Joon Ki Jung
Eui Sung Choi
Chun Suk Kim
Eun Gyo Lee
Ji Yeon Hong
Hyeok Won Lee
Myoung Soo Park
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Korea Research Institute of Bioscience and Biotechnology KRIBB
Original Assignee
Korea Research Institute of Bioscience and Biotechnology KRIBB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Korea Research Institute of Bioscience and Biotechnology KRIBB filed Critical Korea Research Institute of Bioscience and Biotechnology KRIBB
Publication of DE602006018054D1 publication Critical patent/DE602006018054D1/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Disclosed are a Pichia pastoris-derived translation elongation factor (TEF) promoter, a recombinant expression vector carrying the promoter and a heterologous protein encoding base sequence operably linked to the promoter, and a host cell transfected or transformed with the recombinant expression vector. Also, a method is provided for producing a heterologous protein. The method comprises culturing the host cell to express the Pichia pastoris-derived TEF promoter and isolating the promoter from the culture. The TEF promoter can be utilized for the mass production of heterologous proteins without the need for inducers.
DE602006018054T 2005-11-16 2006-01-19 PICHIA PASTORIS TRANSLATION ELONGATION FACTOR PROMOTER AND METHOD FOR PRODUCING RECOMBINANT PROTEIN USING THEREOF Active DE602006018054D1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20050109697 2005-11-16
PCT/KR2006/000215 WO2007058407A1 (en) 2005-11-16 2006-01-19 Translational elongation factor promoter from pichia pastoris and method for producing recombinant protein using the same

Publications (1)

Publication Number Publication Date
DE602006018054D1 true DE602006018054D1 (en) 2010-12-16

Family

ID=38048774

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602006018054T Active DE602006018054D1 (en) 2005-11-16 2006-01-19 PICHIA PASTORIS TRANSLATION ELONGATION FACTOR PROMOTER AND METHOD FOR PRODUCING RECOMBINANT PROTEIN USING THEREOF

Country Status (6)

Country Link
US (1) US7816509B2 (en)
EP (1) EP1951877B1 (en)
KR (1) KR100752107B1 (en)
AT (1) ATE486944T1 (en)
DE (1) DE602006018054D1 (en)
WO (1) WO2007058407A1 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100717353B1 (en) * 2006-04-12 2007-05-11 한국생명공학연구원 Auto-inducible sodium phosphate symporter promoter from pichia pastoris and method for producing recombinant protein using it
RU2560254C2 (en) 2008-06-17 2015-08-20 Индиана Юниверсити Рисерч Энд Текнолоджи Корпорейшн GLUCAGON ANALOGUES, POSSESSING HIGHER SOLUBILITY AND STABILITY IN BUFFERS WITH PHYSIOLOGICAL Ph VALUES
EP2258855A1 (en) 2009-05-28 2010-12-08 Universität für Bodenkultur Wien Expression sequences
DK3508494T3 (en) 2011-10-07 2022-05-23 Lonza Ag ADJUSTABLE PROMOTER
EP3527667B1 (en) * 2013-03-08 2020-11-25 Biogrammatics, Inc. Yeast promoters for protein expression
KR102128959B1 (en) 2013-03-15 2020-07-02 론자 리미티드 Constitutive promoter
US9150870B2 (en) 2013-03-15 2015-10-06 Lonza Ltd. Constitutive promoter
JP2022502018A (en) 2018-09-24 2022-01-11 オルタ ドグ テクニク ユニヴェルシテシ Modified AOX1 promoter variant
EP3824083A4 (en) 2018-09-24 2022-01-05 Orta Dogu Teknik Universitesi Design of alcohol dehydrogenase 2 (adh2) promoter variants by promoter engineering
CN114774461B (en) * 2022-04-06 2023-05-26 暨南大学 Application of Ash1p as negative regulatory factor in improving protein expression in host cell
CN114657197B (en) * 2022-04-06 2023-07-21 暨南大学 Application of Gsm1p as positive control factor in improving protein expression in host cell
CN118308360B (en) * 2024-06-07 2024-08-16 中国人民解放军军事科学院军事医学研究院 Promoter for driving gene to express human serum albumin in yeast and application

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5989870A (en) * 1986-04-30 1999-11-23 Rohm Enzyme Finland Oy Method for cloning active promoters
US5650294A (en) 1990-06-25 1997-07-22 Basf Aktiengesellschaft Isolated promoter and terminator of elongation factor 1-α
US5612198A (en) 1990-09-04 1997-03-18 The Salk Institute Production of insulin-like growth factor-1 in methylotrophic yeast cells
AU2887897A (en) * 1996-05-21 1997-12-09 Novo Nordisk A/S Novel yeast promoters suitable for expression cloning in yeast and heterologous expression of proteins in yeast
ID23301A (en) 1998-04-23 2000-04-05 Hoffmann La Roche EXCESSIVE PRODUCTION FROM RIBOFLAVIN IN THE YEAR
US20030064437A1 (en) * 2000-11-15 2003-04-03 Nick Wan Expression system for recombinant proteins
KR100646100B1 (en) 2004-11-27 2006-11-15 한국생명공학연구원 Method of Screening Novel Promoters from Microorganisms

Also Published As

Publication number Publication date
EP1951877A1 (en) 2008-08-06
ATE486944T1 (en) 2010-11-15
EP1951877A4 (en) 2009-02-18
WO2007058407A1 (en) 2007-05-24
US7816509B2 (en) 2010-10-19
US20090298122A1 (en) 2009-12-03
EP1951877B1 (en) 2010-11-03
KR100752107B1 (en) 2007-08-24
KR20070052179A (en) 2007-05-21

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