DE2126204A1 - Lysozyme preparations - Google Patents

Description

Dr ΓΖΓ · π'.ιϊ · / '/ »' ■ '■" O 1 OCT Π /

8 Munich 22, Maximilians «. 43 , ZIZOZ IM

P 5450-35 26th Hai 19? 1

Lysozyme Products, Inc. 910? Wilshire Boulevard Suite 706 Beverly Hills. California 90210 USA

¹¹ lysozyme preparations ".

The invention relates to new, improved lysozyme preparations containing lysozyme and / or at least one pharmacologically acceptable Lysozyme salt- contain.

Lysozyme is an enzyme protein that 3ir Alexander Fleming discovered by chance 6 years before his discovery of penicillin became. It was named lysozyme because of its rapid dissolving properties

Has an enzymatic effect (lysis) on certain bacteria Effect (Zyme) has. Lysozyme was first discovered in mass secretions, but it was soon discovered that it is also found in various other body fluids, such as tears, saliva, bronchial secretions, etc., as well as in the white ones Blood cells, in the skin, in nails and other parts of the body or. Tissues is present. Again a short time later it was found that as a side effect of certain diseases an annornally low lysozyme content in tears, the white ones Blood cells and elsewhere. Certain attempts at therapy have therefore been undertaken in order to treat conditions or diseases those who have abnormally low levels of lysozyme, lysozyme to feed.

109850/1837

Despite more than two decades of efforts to develop lysozyme therapeutj; .ch and to use as an additive to various preparations, The results obtained were often discouraging and fulfilled Fleming's epoch-making discovery did not save expectations.

The invention is therefore based on the object of creating a method for increasing the biological effects of lysozyme, and accordingly composed lysozyme preparations are available to deliver.

Further objects, features and advantages of the invention emerge from the following description and the claims,

It has now been found that the invention on which the invention is based Solve the problem with a three-component lysozyme base preparation can be obtained by mixing lysozyme and / or at least one pharmacologically acceptable lysozyme salt, such as lysozyme hydrochloride, acetate, sulfate or the like, with hydrogen peroxide and as the third component, cysteine, citric acid, ascorbic acid, Tartaric acid and / or at least one simple salt of the acids mentioned, such as the corresponding ones. Sodium, potassium, Lithium, ammonium or triethylolammonium salts can be produced.

The subject of the invention is thus a Ly s ο zyra preparation from

a) Lysozyme and / or at least one pharmacologically acceptable Lysozyme salt (component a),

b) hydrogen peroxide (component b)

and

109850/1837

BAD OBIGiNAL

c) citric, tartaric and / or ascorbic acid and / or at least an ITatriuM-, potassium-, lit: ium-, ammonium- or 'Iriäthylolammoniumπalζ at least one of the acids mentioned and / or cysteine (component c) as essential constituents.

According to a preferred embodiment of the invention, Lysozyni base preparations of the type described above can be added by mixing with other ingredients and in particular by dispersing, dissolving and the like. In secondary preparations or carriers, such as toothpaste, tooth powder, lozenges, eye washes, nasal sprays, ointments, creams, or Lotions, baby food { and the like are transferred or incorporated.

The relative proportions of the ingredients forming components a) to b) in the present invention Lysosym base preparation can vary within considerable limits without affecting the beneficial properties of the lysozyme preparations according to the invention are lost. In general lysozyme preparations according to the invention should contain component b), i.e. hydrogen peroxide in an amount of about 0.25 to about 30 parts by weight per part by weight of component a) and the ™ Component c), e.g. sodium citrate, tartaric acid, cysteine, etc., im Case of citric acid, citrates, v / one acid, tartrates and / or Cysteine in an amount of about 0.5 to about 50 parts by weight per part by weight of component a), and in the case of ascorbic acid and / or ascorbates per part by weight of component a) in an amount of about 0.02 to about 2 parts by weight.

Since lemon, l. ^r e5n- and ascorbic acid all ratio ·.; - ru. ·; ·: i-.chwitoho or _: '-; anir: chf- neuron cind, Γ1ί. · ^ · η. of course in each \ i;'■; · .srs ·!. ~

10Ph "- ■; 7 i η Ά 7

BATH ORIGINAL

"> 212620A

In order to dissolve these acids and / or their simple salts several different types of ions, their-relative amounts - or. Amounts- " If the p ^ value of the solution and the Ionization or dissociation constants of the relevant acids can be calculated without further ado. It is therefore almost A matter of taste, whether one is involved in the production of a lysozyme preparation that described above, for example, citric acid used and the ρττ-V / ert then with, for example Sodium hydroxide adjusts, or whether to use equivalent amounts of citric acid and sodium citrate to achieve a desired or to achieve the selected p ^ value. Be under simple salts in the context of the invention essentially the sodium, potassium, Understood lithium, ammonium and triethylolammonium salts. Ss it should also be noted that triethylolammonium citrate, -tartrate, etc. usually, albeit incorrectly, otherwise referred to as triethanolamine citrate, triethanolamine, etc.

Various inventions are set out in Table I below Lysozyme preparations and their effectiveness in simple aqueous Solution against different types of bacteria reproduced · ■

109860/1837

-C-

Tabol le I

A "btb'tun _f ~ different bacteria by liquid

mäese lysozyme preparations no; Lysozyme system ρ

Poeudomonas Aeruginosa

Staphylococcus an re vi ο

GoIi

33.

Sub ti Ii s

LY 10 g / ml 3 ,1 1 Min. $ 73 $ 85 $ 98 $ 76 (a) AA 20 <tyg / ml
HgOg 300ug / ml 3 ,1 30th There. $ 100 $ 100 $ 100 $ 93 LY lOug / ml
AA 20 g / ml
H ₂ Og ^ OOug / ml - 3 ,1 1 Min. $ 86 $ 95 $ 91 $ 76 LY lOyg / ml
AA 20 g / ml
HgOg 3mg / ml. 3: ,1 30th Min . $ 100 $ 100 . $ 100 $ 90.7 (c) LY lOug / ml
AA 20 g / ml 3, ,1 1 Min . $ 100 $ 97 $ 100 $ 70 (d) LY ΙΟΟμβ / ml
AA 20 ig / ml 3, ,1 30th Min. $ 100 $ 100 $ 100 $ 100 (e) LY 100 ^ g / ml
AA 20 ^ g / ml
HgOg 3mg / ml 3, , 7 1 Min. • $ 97 $ 100 $ 83 $ 92 (f) LY 100 ug / ml
Z 500 g / ml
HgOg 1 mg / ml 3, 5 30th Min. $ 100 $ 100 $ 100 $ 92 (G) LY 100 ug / ml
Z 1 mg / ml
HgOg 1 mg / ml >. 1 Min. $ 97 $ 15 $ 81 $ 99.2 (H) LY 100 ug / ml
Z 5mg / ml
H ₀ O ₀ lmE / ml
CC 4, 4th 30th Min. $ 100 $ 35 $ 100 $ 99.6 (i) LY 100 ug / ml
Cysteine 5mg / ml
HgOg 1 mg / ml 4, 3 1 Min. $ 96 $ 84 $ 100 U) LY 100 ^ g / rnl
Glycine 5mg / ml
HgOp 1 mg / ml 30th Min. $ 100 $ 91 $ 100 Oc) 1 Min. $ 100 $ 92 $ 100 30th Min. $ 100 $ 100 $ 100 1 Min. $ 100 $ 93 $ 100 30th Min. $ 100 $ 100 $ 100 1 Min. $ 100 $ 100 $ 100 30th Min. $ 100 $ 100 $ 100 1 Min. $ 100 $ 97 $ 100 30th Min . $ 100 $ 100 $ 100 1 Min. $ 100 $ 15 $ 12 30th Min. $ 100 $ 25 $ 20

109850/1837

Table I continued

ITr. Lysozyme system p _t j Pseudomonas Staphylo- E. 33.

Aeruginosa coccus CoIi Subtilis aureus

(D LY 100 ug / ml
Tartaric acid 3> 7
5mg / ml
H ₂ O ₂ Lng / ml 1 Min. 1005-S $ 100 $ 96 $ 100 (m) LY lmg / ral,,
AA 20ug / ml ^ ^
H ₂ O ₂ '300 pg / ml 30th There. 100 * $ 100 $ 100 $ 100 LY
AA
H ₂ O ₂
Z
us 1 Min. $ 98 $ 94 $ 100 30th There. $ 100 $ 100 $ 100 = Lysozyme
= Ascorbic acid
= Hydrogen peroxide
= Citric acid
= 10 "grams -

In the table above and in Table II below, "LY" denotes lysozyme, "AA" denotes ascorbic acid, "H ₂ O ₂ " denotes hydrogen peroxide and ¹¹ Z "denotes citric acid. Various other components are written out in full it can be seen that the three-component lysozyme preparations according to the invention have a remarkable bactericidal effect.

Table II shows the effectiveness of a lysozyme system or lysozyme preparation according to the invention, that of a Zalmpastemisehung is incorporated, reproduced.

The toothpaste used to carry out the experiments int analog of recipe C-I, which is fully detailed below is specified.

1098 50/1837

Table II

Killing of bacteria by lysozyme in toothpaste (Serb and crater test a)

j Ii i, KT]] RI ^ E. CoIi B.
Sub-
tilis Strept.
Sanguis Strepto
coccus
With is Lacto-
bacter
Acido
philus VJl PUITGI Hong Kong·
Flu-
virus VI R ^ . - I lysozyme
[system, pseudo
monas
Ae ru-
ginosa Staphilo-
COCCUS
Aureus 3 ■ Candi
since Al-
bicans 0 min.
IO ⁶ ' ⁰ -Hert> it-
S in
plex
Virus- Polio-
virus IYO, 6mg / ml 2 0 2 5 Hin.
ΙΟ ⁴ ' ⁷ ΙΟ ⁶ ' ³ ΙΟ ⁷ ' ⁷ H ₂ Op 0.6mg / ml 2 7th traces 10 min.
ΙΟ ⁴ ' ³ ΙΟ ⁵ ' ⁷ ΙΟ ⁷ ' ⁵ Z p, 6mg / ml ΙΟ ⁵ ' ⁵ 1O ⁷ ' ⁰

Tis' ^ ts: disks and craters on or in agar plates Results: Measurements in mm in inhibition zones

CO OO CJl O

OO c *>

From the results of the search in the Sauelle II a.ui '^ cfilhi-t..ii it can be seen that in six of the eight etected species excellent inhibition was obtained. Moreover, it turned out The toothpaste mixture has proven to be an effective means of reducing virus concentration in Hong Kong-flu-virus-and Herpes simplex virus cultures and also showed one weak activity against poliomyelitis viruses.

A series of formulations is shown below which illustrate the use of lysozyme base preparations according to the invention in various cosmetic, sanitary and medicinal products, such as Nasal sprays, dentifrices, solutions for the treatment of Explain eyes and the like.

Lysozyme bladder irrigation solutions Recipe 1

Lysozyme hydrochloride 0.20 g

Hydrogen peroxide 0.20 g

Sodium scorbate 0.20 g

Sodium chloride. 0.60 g

Tartaric acid 0.10 g

EDTA (disodium salt) 0.10 g

Acetic acid 0.10 g

Distilled water, make up to 100.00 ml

Recipe 2

Lysozyme hydrochloride 0.20 g

Hydrogen peroxide 0.20 g

Lactic acid 0.10 g

G-lycin hydrochloride 1.00 g

Sodium ascorbate 0.20 g

Thiourea 0.01 g

Distilled water, tinted to 100.00 ml

109850/1837

ORIGINAL BATHROOM

2126204 0.06 g Recipe 3 0.06 g Lysozymic hydrochloride 0.06 g Hydrogen peroxide 0.10 g citric acid 0.06 g Methyl paraben 0.80 g menthol 18.00 g Hydroxyethyl cellulose (250 H) 3.00 g Glycerin 0.12 ml Oxyethylene-oxypropylene polymer 1.00 g peppermint oil Titanium dioxide

Calcium phosphate (dibasic dihydrate) 51.00 g Demineralized water, supplement to 100.00 g

Recipe 4

Lysozyme hydrochloride 0.06 g

Hydrogen peroxide 0.06 g

Ascorbic acid "'..-; ■ 0.06 g

Methyl paraben 0.01 g

Menthol 0.06 g

Hydroxyethyl cellulose (250 H) 0.80'g

Glycerin 18.00 g

Oxyethylene-oxypropylene polymer 3.00 g

Peppermint oil '0.12 ml

Titanium dioxide 1.00 g

Calcium phosphate (zv / eibasic dihydrate) 51.00 g Demineralized water, supplement to 100.00 g.

10 98 507 1,837

Formulation 5 lysozyme hydrochloride hydrogen peroxide ascorbic acid citric acid methyl paraben menthol

Hydroxyethyl cellulose (250 H) glycerine, oxyethylene-oxypropylene polymer Peppermint Oil! Titanium Dioxide

Calcium phosphate (zveibaseehes dihydrate Entraineralized water, supplement on

Recipe 6

.Lysozyme hydrochloride hydrogen peroxide Citric acid "methyl paraben menthol

Hydroxyethyl cellulose (250 K) '""' Glycerin

Oxyethylene-oxypropylene polymer
Pfefferrain'zö'l ^! '

Titanium dioxide

Calcium phosphate (sv: egg-based dihydrate) Demineralized V.'asser, supplement on

2126204 0.06 Oj 06 - τ- ■
O - - "- = 0.06 g -ν ': - 0.06 G 0.10 g -. ■■ '■■ - 0.06 g; : -. v 0.80 G 18.00 S. 3.00 S- 0.12 ml 1.00 G 51.00 G 100.00 G 0.06 G 0.12 g '" 0.06 G 0.10 G 0.06 i ''"'~ ^: 0.80 G;' 18.00 g ' ■■''■■■' ■ '· ■ "■ 3.00 ^: G 0.12 1.00 51.00. g; - 100, QO. g -, '..- ■ -' = - ■ - ■■.

109850/1837

-Mr

Recipe 7

Lysozyme hydrochloride - 0.12 g

Hydrogen peroxide 0.24 g

Citric acid 0.12 g

Methyl paraben 0.10 g

Menthol 0.06 g

Hydroxy: ethyl cellulose (250 II) 0.80 g

Glycerine 18.00 ml

Oxyethylene-oxypropylene polymer 3.00 g

Peppermint oil 0.12 ml

Titanium dioxide 1.00 g calcium phosphate (dibasic dihydrate) 51.00 g De-mineralized water, supplement to 100.00 g

Recipe 8 (artificial tears)

Lysozyme hydrochloride 0.500 g

EDTA (disodium salt) 0.010 g

Hydrogen peroxide 0.010 g

Sodium ascorbate 0.100 g

Sodium chloride 0.800 g

Naphthazoline hydrochloride 0.020 g sodium phosphate (dibasic, anhydrous) 0.038 g

Polyvinyl alcohol (51-05, Dupont) 1,000 g

Pluronic F68 (uyandotte) 0.250 g

Distilled water, make up to 100,000 ml

Recipe 9

Lysozyme hydrochloride 0.500 g

EDTA (disodium salt) 0.010 g

Hydrogen peroxide 0.010 g

Sodium ascorbate 0.100 g

Sodium chloride 0.800 g

ITaphthazoline hydrochloride 0.020 g hatriuin phosphate (zv / eiba-sisch, anhydrous) 0.038 g

Ilethyl cellulose (4COO cPs., 65 Hg) 0.250 g

Pluronic P60 (Vyandotte) 0.250 g

Distilled water, make up to 100,000 ml

1098B0 / 1837

2126204 0.30 g Recipe 10 (Lysozvn smoke drops) 0.01 g Lysozyme hydrochloride 0.01 g EDTA (disodium algae) 0.01 g Hydrogen peroxide 0.01 g Thiourea 0.02 g Sodium ascorbate 0.01 g Naphthazoline hydrochloride 1.50 ml Sodium tetrapyrophosphate 1.00 g Glycerin 100.00 ml Polyvinyl alcohol (51-05, Dupont) Distilled v / ater, supplement on 0.30 g Recipe 11 0.01 g Lysozyme hydrochloride 0.01 g EDTA (disodium salt) 0.01 g Hydrogen peroxide 0.01 g Thiourea 0.02 g Sodium ascorbate 0.01 g Naphthazoline hydrochloride 1.50 ml Sodium tetrapyrophosphate 0.25 g Glycerin 100.00 g Methyl cellulose (4-00G cPs. 65 Hg) Distilled water, supplement on 0.10 g Recipe 12 (Lysozyme Shampoo) 0.02 g lysozyme hydrochloride 2.00 g Hydrogen peroxide 30.00 g Crodafos I ^ acid 1.00 g Miranol 2MCA-ESF * 0.30 g Hydroxyethyl Cellulose (250) 100.00 g citric acid Distilled water, supplement on

109 850/1837

Lysozyme hydrochloride 0.10 g

Hydrogen peroxide 0.01 g

Citric acid 0 ', 30 g

Hydroxyl ethyl cellulose 2.00 g

Triton X-200. '32, 0Og

Demineralized '.'water, supplement to 100.00 g

* (Togo "b8tain can be used instead of Hiranol 2MGA-ESF)

Prescription ur 14 Lysozyme Hydrochloride Hydrogen Peroxide lTatriu: nascor "asked Thiourea EDTA (disodium salt) ITorephedrine Chlorprophenpyridaminnaleate Methylparaben Distilled water, supplement on

Formulation 15 (Lysozyra-ITasen s pray) Lysozyme hydrochloride Hydrogen peroxide I Sodium ascorbate Thiourea EDTA (disodium salt) Horephedrine Chlorprophenpyridaminnaleate Hettiylparahen Distilled water, supplement on

•1 , 00 , 00 G 1 , 00 , 20 G 0 , 20 , 10 S. 0 , 10 , 10 S. 0 , 10 00 S. 3; , 00 50 G , 50 10 G • o. , 10 00 G 100, , 00 ml 100 G 1, G O, G O, G O. S. 3, G O, G O, G 100, ml

109850/1837

Recipe 16 (acne gel) BenzoylpejfDicid Hydrogen peroxide Lysozyme hydrochloride Citric acid Ethanol

Hydroxyethylellulose (250 H) Distilled water, supplement on

Recipe 17

Hydrogen peroxide lysozyme hydrochloride citric acid Allantoin

Ethyl alcohol Hydroxyethyl cellulose (250H) Distilled water, supplement on

Recipe 18 (acne lotion)

Hydrogen peroxide lysozyme hydrochloride citric acid EDTA (disodium salt) Precipitated sulfur allantoin

Carbopol 934 triethanolaniine Isopropyl alcohol Distilled water, supplement on

Recipe 19 (Taginal shower)

Lysozyme hydrochloride Citric acid Efodium Perborate Lactose

Distilled water, supplement on

109850/1837

, 0 2126204 ml 5 , 0 G 200 g 1 , 3 G 000 g 0 , 5 G 000 g 0 , 0 S. 000 g 25th , 0 G 000 ml 3 , 0 G 100 , 0 ml 1 , 3 G 0 , 5 G , 0 G 2 , 0 G 25: , 0 G 3: , 0 G 100, , 0 ml - I ₁ , 3 G 0, , 5 G , 2 G O, , 0 G .3, , 0 G 2, , 0? G 2, , 0 G 2, G 25, 00g 100, 0 O, 2, 2, 2, 100,

The trade sums used in the tables above, Abbreviations and the like have the following meanings:

EDTA / ethylenediaminetetraacetic acid (sodium salt)

Pluronic F68 condensate of poly (ethylene oxide) with a

.hydrophobic base, molecular weight approx. 8000.

Pharraagel A Gelatin, Kr. 1 U.S.P.

Carbopol 934 polyacrylic acid

Crodafos N ^ -acid alkoxyphosphate with long-chain alkyl residues

Hiranol 2 KCA-ESP dicarboxy- (long-chain) -alkylimidazolinium-

sulfate

Triton X-222 long chain alkyl aryl 1-polyether sulfonate

(Sodium salt).

The lysocyin preparations according to the invention can be used in a number of ways various products, some examples of which are given below:

Products to be used orally:

Toothpaste, mouthwash, lozenges, chewing gum and other types of ' Dentifrices and denture cleaners.

Products intended for eye treatment:

Antiherpes eye drops, artificial tears, eye drops against Eye irritation from smoke, contact lens preparations for use with hydrophilic and hydrophobic contact lenses and antibacterial ones Eye drops used to treat various eye infections.

Rabbit sprays:

Sprays to treat various viral (flu or runny nose) and bacterial nasal infections.

109850/1837

Applicable for treating skin dor Prclr.kte: - skin conditioning agents, and -befeuchtungsmittel, Akncnittel, shampoos, soaps, face painting and agents for the treatment of viral (zona herpes) and other various skin diseases such as dermititis, Psorizis and so on.

Preparations for the treatment of ear infections, both viral and bacterial Origin (otitis).

CTfood! Additives:

Additive to cow's milk to "humanize" the latter.

Radiation protection agent:

Funds for patients suffering from various types of cancer who require therapeutic treatment with X-rays and gamma rays or to treat patients with radiation sickness.

Means against viral diseases:

Agent against viral hepatitis, herpes simplex and zona zoster.

Veterinary application;

Treatment of foot and mouth disease (viral disease).

Remedies against Pungae ^ (mycosis)

Candida albicans, Kundschinitis and Mycosis dor

Oral cavity, skin and vaginal tract.

Disinfectant:

Agents for medical laundry and bladder rinsing agents.

Products for indus triel le applications , ^ sK cigarette filters xxnä anti-P treated with Lycozym

1098bU / 1837

Sugar.

It should be noted that lysozyme preparations made according to the invention are noteworthy are persistent. In a dilute aqueous solution, the peroxide analysis is on the order of magnitude over a period of time see only a slight decrease over a few months. It is believed that on a not yet fully clarified Way, the various components of the lysozyme preparation according to the invention stabilize the peroxide against decomposition, what of course with preparations intended for use in hospitals and even with preparations for general use commercial purposes is a great advantage.

As can be seen from the above, can the lysozyme (basic) preparations of the invention according to the invention can be produced and supplemented in various ways, with end products being expediently in the form of powdered mixtures or from solutions in water, ethyl alcohol, glycerine or the like. You can also use the lysozyme preparations of the Formulate the invention by appropriate selection of the components according to type and amount so that it is measured in aqueous solution pjT-Vert any desired value in the range of about 2 to about 9 has. As a rule, lysozyme preparations according to the invention are preferred which have a Prr value of about 5 to 8, as these preparations with physiological conditions, e.g. the normal p "value of the skin, of mucous membranes, tears, Saliva and the like are particularly well tolerated.

10985 0/1837

eat

* ⁷ 2126ΫΘ4

Furthermore, it was stated that the ly sozyme (basic) preparations according to the invention were used as aucsserordontlieh valuable additional components in a large number of mixtures or Priipa-f rates for pharmaceutical, therapeutic, cosmetic and similar applications are suitable.

It should also be noted that the invention is based on Hand of special components, "certain proportions, Concentrations, optionally to be added further components and the like has been explained, but of course is not restricted to the details of the examples described and is within the scope of the invention for the person skilled in the art numerous obvious modifications are possible.

109850/18 37

Claims (11)

P 34-50-35. May 26, 1971 o_ claims 1. Lysozyme preparation from a) Lysocyin and / or at least one pharmacologically harmless!: - borrowed lysozyme salt (component a), b) hydrogen peroxide (component b), and e) Lemon, V. ^r and / or ascorbic acid and / or at least one sodium, potassium, lithium, ammonium or triethylol ammonium salt of at least one of the acids mentioned and / or cysteine (component c)
as essential components. 2. lysozyme preparation according to claim 1, characterized in that that there is 0.25 to 30 parts by weight per part by weight of component a) of component b) and, if component c) is ascorbic acid and / or an ascorbate, 0.02 to 2 parts by weight, in all in other cases, contains 0.5 to 50 parts by weight of component c), the composition being chosen so that the pj- ~ V / ert an aqueous solution of the lysozyme preparation in the range of 2 to 9. 3. Lysozyme preparation according to claim 2, characterized in that that there is citric acid as component c) and / or at least contains a citrate. 4. Lysozyme preparation according to claim 2, characterized in that that it contains tartaric acid and / or at least one tartrate as component c). 109850/1837 5. Lysozyme preparation according to claim 2, characterized in that that it contains ascorbic acid and / or at least one ascorbate as component c) » 6. Lysozyme preparation according to claim 2, characterized in that that it contains cysteine as component c). 7 .. lysozyme preparation according to at least one of claims 1 to 6, characterized in that it is dry and powdery and contains at least one powdered mineral abrasive as a further component, in which the components a) to e) are dispersed. .8 * lysozyme preparation according to claim 7, characterized in that that it contains dicalcium phosphate as a mineral abrasive. 9 »Lysozyme preparation according to at least one of claims 1 to 6, characterized in that it is used as a further component V / water in which components a) to c) are dissolved in such an amount that the concentration of component a) in the aqueous solution is 10 mg to 10 g / liter. 10. lysozyme preparation according to claim 9 »characterized in that there is at least one powdered as additional components mineral abrasive and at least one water swellable hydrophilic polymer in an amount sufficient to to thicken it into a paste, i.e., "to give it a consistency where it will not run, but still is extrudable. 1 09 850/1837 11. Use of lysozyme preparations after at least one of claims 7 "to 10 as a tooth cleaning agent. 109860/1837