DE102011000891A1 - Method for determining variables of sample e.g. fluid in container, involves determining resulting state of movement of sample produced by movement of container - Google Patents

Abstract

The method involves moving a container (2) in a defined manner, emitting electromagnetic signal on sample (1) inside the moving container. The optical response from the sample is detected, so as to determine a variable of the sample. The resulting state of movement of the sample produced by the movement of container is determined at the time of emission of electromagnetic signal on the sample. An independent claim is included for device for determining variables of sample.

Description

The invention relates to a method and an apparatus for determining at least one variable of a sample. In particular, the invention relates to a method and apparatus for determining at least one variable of a sample which is in a moving container.

In a variety of studies in various fields, such as chemistry, pharmacy or the life sciences, samples are analyzed in containers, which are moved to support the processes of interest in a particular investigation. This is preferably done mechanically; The person skilled in the corresponding devices such as shakers, shakers or rockers are well known. Such devices are commercially available in embodiments which can move a container, but also a plurality of containers simultaneously in a defined manner. The purpose of the movement is usually a thorough mixing of the sample, which is present as a fluid medium or liquid, for example solution, emulsion or suspension; The sample may also be a fluid medium in which microorganisms develop.

For example, the article describes "Combination of On-line pH and Oxygen Transfer Rate Measurement in Shake Flasks by Fiber Optic Technique and Respiratory Activity Monitoring System (RAMOS)", in Sensors, 2007, 7, 3472-3480 , the measurement of pH and oxygen transfer in a solution containing E. coli. The solution is in several containers on a shaker. The containers are only partially filled, so that there is a gas space above the solution. The oxygen transfer is determined by analysis of the gas mixture in the gas space, the pH value via a fluorescent pH sensor, which is optically excited and read out via a glass fiber.

The contribution of A. Azizan, R. Voravichan and J. Büch's "Optimizations and Interpretations of Signal from Liquid Distribution Setup in Shake Flask" in Proceedings of the World Congress on Engineering 2009 Vol II WCE 2009, July 1-3, 2009, London, UK deals with the fluid profiles that form in the fluid within a container moved by a shaker. The dependence of the liquid profile on shaking frequency, radius of the shaking movement, level of the container and viscosity of the liquid is examined. For the purpose of the investigation, a fluorescent liquid is used which is optically excited by a plurality of glass fibers, and whose fluorescence is detected by the glass fibers and passed to the evaluation.

When measuring samples in a moving container, it is important to ensure that each measurement is performed under defined conditions, otherwise the quality of the measurement results will be reduced. Due to the forming of the movement of the container profile of the fluid sample within the container such defined conditions are not a matter of course. If, for example, the absorbance of the sample is to be determined by means of a light beam directed into the sample, it is important to know whether relatively much or relatively little sample material is located at the region of the profile onto which the light beam is directed to determine the extinction. If such examinations are carried out repeatedly, for example to improve the signal-to-noise ratio or to determine the time course of a change in the sample, significant systematic errors can occur if the profile formation in the fluid sample is ignored.

It is therefore an object of the invention to provide a method with which at least one variable of a sample located in a moving container can be determined reliably and reproducibly.

This object is achieved by a method according to claim 1.

It is a further object of the invention to provide a device with which at least one variable of a sample located in a moving container can be determined reliably and reproducibly.

This object is achieved by a device according to claim 13.

In the method according to the invention for determining at least one variable of a sample located in a moving container, the container is moved in a defined manner. According to the invention, the determination of the at least one variable is coordinated in time with a movement state of the sample resulting from the movement of the container.

In the method according to the invention, parameters for determining the movement of the container, characteristics of the container itself and properties of the sample are advantageously used for timing the determination of the at least one variable. Characteristics of the movement of the container, for example, if the container is moved by a shaker, shaking frequency and amplitude of the shaking motion. Characteristics of the container are, for example, its shape, surface texture, and the level. One An example of a particularly important property of the sample is its viscosity. The significance of the variables mentioned is that they have a significant influence on the movement state of the sample resulting from the movement of the container. Characteristics of the movement of the container can preferably be detected by sensors, the skilled person are various ways known for this purpose, for example, position sensors or accelerometers.

In embodiments of the method according to the invention can be determined from characteristics of the movement of the container, characteristics of the container itself and properties of the sample temporal shifts that a relationship between the movement of the container and the state of motion of the sample, which thereby for the timing between the determination of At least one variable and the state of motion of the sample is used to represent in a sufficiently precise manner for a given measurement task. The determination of the time shifts can be done for example by a computer. In the case of a given time-periodic movement of the container, these time shifts may assume fixed values for given parameters of the container itself and predetermined properties of the sample, and for example in the form of a constant delay between a time in which the moving container is at a certain position , and the triggering of a measurement process to determine the at least one variable to be implemented.

Advantageously, for the timing of the determination of the at least one variable to the state of motion of the sample information about the state of motion of the sample is included, resulting from at least one measurement on the sample itself during the movement of the container. For example, information about the profile of the sample within the moving container can be obtained optically. This can be done, for example, by evaluating the attenuation of a light signal when passing through the sample, or the backscattering of light emitted into the sample; However, other optical methods are conceivable.

In the timed determination of the at least one variable, if necessary, the duration of switching operations in a device for carrying out the method can be taken into account.

In embodiments of the method according to the invention, the sample is a fluid medium or a liquid; it may be, for example, solutions of substances, suspensions or emulsions. The investigation of fluid media in which microorganisms develop is possible with the method according to the invention. In such samples, a time- and location-dependent accumulation of the fluid medium or the liquid can form in the container by the state of motion of the sample. The timing of the determination of the at least one variable on the state of motion of the sample takes into account a position of accumulation in the container. The formation of the accumulation corresponds in many embodiments to the formation of a fluid sickle, and said timing ensures that the determination of the at least one variable is reproducible within a predetermined range of this accumulation or fluid sickle to ultimately render the at least one variable of the sample reliable and reproducible to determine. In embodiments of the method, the at least one variable is a concentration of a substance, for example of alkali or alkaline earth metals or their ions, a partial pressure of a gas such as oxygen or carbon dioxide, a pH or an extinction or optical density of the Sample.

One possible embodiment for determining at least one variable of the sample is based on an optical method. For this purpose, an electromagnetic signal is emitted into the container, and detects an optical response from the container. At least the emission of the electromagnetic signal is timed to the state of movement of the sample resulting from the movement of the container such that the electromagnetic signal hits the sample in a reliable and reproducible manner. The at least one variable is then determined from the optical response.

In certain embodiments, to determine the at least one variable in the container, at least one sensor is mounted which has an optical behavior which depends on the at least one variable and which advantageously results in a luminescence appearance. The luminescence of the at least one sensor can be excited by an electromagnetic signal emitted into the container.

An electromagnetic signal transmitted into the container may comprise one or more frequency ranges; it can consist of one pulse, but also of several pulses which are directed simultaneously or successively to different regions of the sample or to the same region of the sample. The exact configuration depends on the respective measurement tasks, ie after the one or more variables that are to be determined, as well as after the arrangement thereof optionally used sensors in the container. For example, to determine two variables, such as pH value and oxygen content, two sensors can be used which have a luminescence behavior dependent on one of the variables. These sensors can be fixed at two different locations within the container. In such a constellation, it is now important to match the excitation of a respective sensor in time to the relative position of the sensor with respect to the profile of the sample. It is therefore not only important that the electromagnetic signal hits the sensor in order to stimulate its luminescence, but in particular also that this happens when a certain relative position between the sensor and profile of the sample is given.

In general, it is possible with the method according to the invention to repeatedly determine the at least one variable. As a result, for example, a signal-to-noise ratio can be improved or a temporal change of the at least one variable can be detected. By the inventive timing between determining the at least one variable of the sample and the state of motion of the sample, the systematic errors mentioned above are avoided.

The device according to the invention for determining at least one variable of a sample located in a moving container comprises a carrying element which is suitable for receiving the container. With the support member a defined movement can be executed, so that in this way a defined movement of the container can be achieved. The device according to the invention further comprises a measuring system, by which at least one variable of the sample can be determined. According to the invention, a synchronization means is provided by which the determination of the at least one variable can be tuned in time with a movement state of the sample that can be generated by the movement of the container.

In embodiments, the synchronizing means comprises a position measuring arrangement, for example an encoder, for measuring the position of the carrying element. Instead, or in combination with it, an acceleration sensor for determining the acceleration of the support element can also be provided. With knowledge of the initial position and the initial speed, which is usually zero, can be determined from the measured acceleration by two times integration over time, the position of the support member. In many embodiments of the device, however, the defined movement of the support member is such that it can be concluded from the acceleration determined at a time without integration on the position of the support member. A particularly simple example of this is when the defined movement of the support element is in a circular motion with a given radius. A direction of the acceleration is clearly linked to the position of the support element on the circle. The direction of the acceleration can be determined by determining the accelerations in two linearly independent directions in one plane of the circle. The skilled person is familiar with suitable acceleration sensors; Such acceleration sensors are advantageously mounted on the support member or integrated into the support member.

Advantageously, the synchronizing means comprises means for obtaining information about the state of movement of the sample. In this case, the device preferably works optically, and therefore comprises a suitable optical system.

In embodiments of the device according to the invention, the measuring system, by means of which at least one variable of the sample can be determined, operates optically. In order to emit an electromagnetic signal into the container, the measuring system comprises a controllable light source. Here, light source means any device for emitting radiation from at least a portion of the electromagnetic spectrum. It is also conceivable that light from a plurality of wavelength ranges can be emitted by the light source. In one embodiment, at least one light-emitting diode (LED) is used as the light source. Also, the use of multiple LEDs, which can also be located in a specific geometric arrangement, as a light source is possible. Individual LEDs of the arrangement may differ from one another by the frequency range of the emitted light. The controllability of the light source is relevant to be able to carry out the emission of an electromagnetic signal in time to the state of motion of the sample and thus to adjust the determination of the at least one variable of the sample in time to the state of motion of the sample. The timing is the responsibility of the synchronizer. The measuring system in such embodiments further comprises means for detecting an optical response from the container, and means for determining the at least one variable from the optical response. The means for detecting the optical response may also be timed with respect to their operation to the state of motion of the sample, which in turn may be done by the synchronizing means. For example, both the light source of the measuring system and the means for detecting the optical response by the synchronizing means.

In various embodiments, the light source is designed to cooperate with at least one sensor mounted in the container. The sensor has an optical behavior dependent on at least one variable of the sample; this behavior is preferably in a luminescence phenomenon.

In certain embodiments of the device, the carrying element is designed to receive a single container. In other embodiments, the support member is adapted to receive a plurality of containers. In the latter case, each container can be assigned a controllable light source.

In certain embodiments of the device according to the invention, the carrying element is a plate on which the at least one container can be fixed.

Hereinafter, embodiments of the invention and its advantages with reference to the accompanying drawings will be explained in more detail. Show it:

1 a schematic block diagram of the invention;

2 a perspective view of an embodiment of the invention, which is designed for the use of a container;

3 a perspective view of the essential internal structure of the embodiment 2 ;

4a a sectional view of a container, in which an accumulation of the sample is schematically shown, as it results upon movement of the container;

4b a plan view of the container of 4a ;

5 a sectional view through a container and an associated optical module;

6 a further sectional view through a container and an associated optical module;

7 a perspective view of an embodiment of the invention, which is designed for the use of a plurality of containers (here nine);

8th a perspective view of the essential internal structure of the embodiment 7 ,

For identical or equivalent elements of the invention, identical reference numerals are used. Furthermore, only those reference numerals are used in a drawing, which are required for the description of the respective drawing or their inclusion in the context of the remaining drawings.

1 shows a schematic block diagram of the invention. A sample 1 is in a container 2 , the container 2 turn is from a support element 22 added. The carrying element 22 and with him the container 2 , can perform a defined movement. This can, for example, by a drive 27 which are generated by a control unit 26 is controllable. The defined movement can take place in one, two or three spatial directions; In addition, rotational movements about one or more axes are conceivable.

A measuring system 21 , which preferably on the support element 22 is arranged to be at least one variable of the sample 1 to determine. For this purpose, the measuring system may comprise a data processing unit. It is also conceivable that only subsystems of the measuring system 21 on the support element 22 are arranged while other subsystems, such as a data processing unit, not on the support element 22 are arranged and so in particular the defined movement of the support element 22 do not perform with.

Due to the defined movement of the container 2 becomes a certain state of motion of the sample 1 generated. A synchronizer 5 is intended to determine the at least one variable of the sample 1 on the state of motion of the sample 1 to coordinate the determination of the at least one variable in a reproducible manner. For the purpose of this timing, the synchronizer determines 5 Data on the movement of the support element 22 , and thus to the movement of the container 2 , For this purpose, a position measuring arrangement can be provided, which directly the position of the support member 22 certainly. In 1 is an acceleration sensor 51 provided that the acceleration of the support element 22 determined. Such an acceleration sensor 51 is easy in the carrying element 22 integrated. Furthermore, the synchronization means comprises 5 the embodiment of the invention shown a device 52 for obtaining information about the state of motion of the sample 1 , Preferably, the device uses 52 a optical method to get information about the state of motion of the sample 1 directly, ie without going through, for example, the movement of the support element 22 , to investigate. Embodiments are also conceivable in which either an acceleration sensor 51 or a facility 52 for obtaining information about the state of motion of the sample 1 is provided. However, a combination of the two is generally advantageous. For evaluation by the institution 52 and the acceleration sensor 51 determined data is a computing unit 53 intended. The arithmetic unit 53 is preferably integrated in the synchronizing means, but also alternatives are conceivable in which the arithmetic unit 53 not the movement of the support element 22 with, and is given for example by an external computer. Another essential task of the computing unit 53 It is due to the result of the evaluation of the data from the acceleration sensor 51 and from the institution 52 the measuring system 21 to control the determination of the at least one variable of the sample 1 timed to the state of motion of the sample 1 to induce.

It is of course possible that the arithmetic unit 53 and a data processing unit of the measuring system 21 are given by a single data processing system, that is, that the data processing system both by the synchronizing means 5 as well as from the measuring system 21 is being used. The data processing system can be on the support element 22 be arranged or external.

2 shows a perspective view of an embodiment of the invention, which is for the use of a container 2 is designed. The support element is here a plate 22 ; Such plates are used in shakers. A shaker allows a user to specify a defined movement for the disk, and then executes it. In the container 2 there is a sample 1 , which is a fluid medium here. According to frequently occurring experimental conditions such as chemistry, pharmacy, or the life sciences, is the container 2 only to a small extent through the sample 1 filled. The container 2 is through a bracket 11 on the plate 22 fixed, and therefore performs a defined movement of the plate 22 With. This results from a predetermined defined movement of the plate 22 a known defined movement of the container 2 , In the plate 22 is an optics module 30 integrated, which of the container 2 partially obscured. The optics module 30 forms in the embodiment shown a part of the measuring system for determining at least one variable of the sample 1 which happens optically in the embodiment shown. The optics module 30 includes (see 5 and 6 ) at least one controllable light source and one of the light source respectively associated detector.

3 shows a perspective view of the essential internal structure of the embodiment 2 , In the plate 22 integrated is an acceleration sensor 51 ; this consists of an actual acceleration sensor element, which is very small (typically about 2 mm to 2 mm) and therefore not shown here, as well as the much larger board for driving the acceleration sensor element. The optics module 30 is completely visible here. It includes here three first openings 31 for each one light emitting diode, and a respective first opening 31 each assigned a second opening 32 for a detector. Further, two of the first openings 31 one filter bag each 33 of rectangular cross section, into which optical filters for adaptation of the in the respective first opening 31 located LED emitted light can be introduced to a respective measurement task. There are also a total of three measuring boards 60 shown that part of the electronics for the evaluation of the detectors in the second openings 32 form part of the measuring system for determining the at least one variable of the sample.

A container 2 with a distribution of a sample 1 in its interior, which is due to a state of motion of the sample 1 results as he passes through a defined movement of the container 2 is generated in 4a in cross-section, and in 4b shown in plan view. In the sample 1 it is a fluid medium, and due to the defined movement of the container 2 Forms a local accumulation 3 the height H in an area near the boundary of the container. Other areas of the container 2 In contrast, in comparison to the case of a stationary container, the uniform distribution of the sample 1 over the cross-section of the container to the level corresponds to a reduced amount of the sample 1 on. The formation of such an uneven distribution of the sample 1 inside the container 2 depends on the level and the acceleration of the container 2 ; the latter is determined by the shaking frequency in the case of a shaker.

The 4a and 4b illustrate very clearly the core problem, which is solved by the present invention. Due to the uneven distribution of the sample 1 within the moving container 2 is, in contrast to the case of the stationary container 2 , by no means ensures that a measurement made to determine at least one variable of the sample 1 takes place under reproducible conditions. For example, it is by no means guaranteed during a measurement by optical means that one enters the container 2 directed electromagnetic signal, such as light pulses, the sample 1 at all, let alone in a reproducible way. For example, a first pulse could be the sample 1 completely missing, a second pulse the accumulation 3 and a third pulse could be a sample tail 8th to meet. For the person skilled in the art it is immediately clear that the evaluation of measurements is made considerably more difficult by these circumstances. The present invention overcomes this problem by determining the at least one variable on the state of motion of the sample 1 is timed. In the case of a determination of the at least one variable optically, therefore, at least the emission of the electromagnetic signal, in the described case thus the light pulses, on the movement state of the sample 1 Timed, that is, the location of the accumulation 3 in the container 2 to determine the times at which the light pulses are emitted, is used in accordance with the respective measurement task.

5 shows a section through a container 2 and an associated optics module 30 , In the container is a sensor 4 fixed. In cross section through the optical module 30 are a light emitting diode 6 and a detector 7 shown. The optics module 30 may of course comprise a plurality of light-emitting diodes and associated detectors, on average only one LED at a time 6 and a detector 7 detected. The totality of light emitting diodes 6 an optical module 30 forms a controllable light source through which an electromagnetic signal can be emitted. In the illustration is the light-emitting diode shown 6 arranged so that light emitted by it is not on the sensor 4 is directed. From a sample 1 in the container 2 becomes part of the LED 6 in the container 2 emitted light backscattered. The detector 7 is intended to detect this backscattered light. After evaluation of the detector 7 supplied data is through the measuring system 21 (please refer 1 ) determines an optical density of the sample. To determine the optical density reliably and reproducibly, is a timing of the light emission from the light emitting diode 6 with the state of motion of the sample 1 required in the context of 4a and 4b explained difficulties to avoid, for example, such that a light pulse from the LED 6 is always sent out so that he is the sample 1 in the range of an accumulation 3 the sample 1 meets. In this way it is ensured that the light from the LED 6 always a substantially equal distance in the sample 1 and therefore changes in the extent of backscattering of the light of the LED 6 through the sample 1 not to fluctuations in the sample 1 from the light of the LED 6 covered route but to changes in the composition of the sample 1 are attributed.

As an alternative, the dependence of the extent of the backscatter of the light of the LED can be 6 from the one in the sample 1 track used to optically information about the state of motion of the sample 1 to investigate. In such an embodiment, the LEDs shown are 6 and its associated detector 7 Parts of in 1 shown device 52 for obtaining information about the state of motion of the sample 1 ,

6 shows a section through a container 2 and an associated optics module 30 , In the container is a sensor 4 fixed. In cross section through the optical module 30 are a light emitting diode 6 and a detector 7 shown. The optics module 30 may of course comprise a plurality of light-emitting diodes and associated detectors, on average only one LED 6 and a detector 7 detected. The totality of light emitting diodes 6 an optical module 30 forms a controllable light source through which an electromagnetic signal can be emitted. In the illustration is the light-emitting diode shown 6 arranged so that light emitted by it on the sensor 4 is directed. The sensor 4 has a luminescence behavior that of at least one variable of the sample 1 depends. The luminescence of the sensor 6 is due to the light from the light emitting diode 6 stimulated. From the sensor 4 emitted luminescent light is passed through the detector 7 detected. The light emitted by the light-emitting diode can be optimized for the respective measuring task by placing it in one of the light-emitting diodes 6 associated filter bag 33 a suitable filter is introduced.

In the embodiment shown, the light emitting diode is aligned so that light emitted by it always the sensor 4 meets. However, a reliable and reproducible determination of at least one variable of the sample 1 to enable is a timing of the light emission from the light emitting diode 6 with the state of motion of the sample 1 required in the context of 4a and 4b explained difficulties to avoid, for example, such that a light pulse from the LED 6 always sent out so that he has the sensor 4 always at a time when the area of accumulation meets 3 the sample 1 above the sensor 4 located. In this way, reproducible conditions for the sensor 4 guaranteed.

7 shows an embodiment of the invention, which is based on the use of a plurality of containers 2 is designed. In the illustration are nine containers 2 shown. The containers 2 be from a common support element, this one plate 22 is, worn. The plate 22 can be moved in a defined way. Each container 2 is an optics module 30 assigned, which is partially obscured by the respective container. each container 2 is through a bracket 11 on the plate 22 fixed, and therefore performs a defined movement of the plate 22 With. In every container 2 there is a sample 1 , It is clear to the person skilled in the art that every optical module 30 Advantageously separately controllable, that is, for each optical module 30 is the emission of an electromagnetic signal from that through the LEDs of the optics module 30 given light source on the state of motion of the sample 1 in the respective optical module 30 associated container 2 separately in accordance with the invention temporally tunable, and of course the evaluation is carried out for each container 2 separately.

8th shows the essential internal structure of the plate 22 , The structure of the now fully recognizable optical modules 30 corresponds essentially to the design of the optical module for execution for a single container, as in the context of 2 and 3 has been described in detail. Further, an acceleration sensor 51 also shown in the context of 3 was discussed. For evaluation of the detectors in the optics modules 30 supplied data is a measuring board 60 intended.

The invention has been described with reference to certain embodiments. However, it will be understood by those skilled in the art that changes and modifications of the embodiments may be made without departing from the scope of the following claims. It should be stressed in particular that, although the 2 to 8th refer to the use of the invention in shakers, the invention is not limited to shakers. Likewise, the invention could be used for example in rockers and other similar devices.

QUOTES INCLUDE IN THE DESCRIPTION

This list of the documents listed by the applicant has been generated automatically and is included solely for the better information of the reader. The list is not part of the German patent or utility model application. The DPMA assumes no liability for any errors or omissions.

Cited non-patent literature

• "Combination of On-line pH and Oxygen Transfer Rate Measurement in Shake Flasks by Fiber Optic Technique and Respiratory Activity Monitoring System (RAMOS)", in Sensors, 2007, 7, 3472-3480 [0003]
• A. Azizan, R. and J. Voravichan Büchs "Optimizations and Interpretations of Signal from Liquid distribution setup in Shake Flask" in Proceedings of the World Congress on Engineering 2009 Vol II WCE 2009, July 1-3, 2009, London, UK [ 0004]

Claims (23)

Method for determining at least one variable in a moving container ( 2 ) ( 1 ) comprising the following steps: a) moving the container ( 2 ) in a defined manner; and b) determining the at least one variable, wherein the determination coincides with a time determined by the movement of the container ( 2 ) resulting movement state of the sample ( 1 ). Method according to claim 1, wherein for the timing in step b characteristics of the movement of the container ( 2 ), Parameters of the container ( 2 ) itself and properties of the sample ( 1 ) are used. Method according to claim 2, wherein parameters of the movement of the container ( 2 ) are sensory detected. Method according to one of the preceding claims, wherein, for the timing in step b, information about the state of movement of the sample ( 1 ) resulting from at least one measurement on the sample ( 1 ) even during the movement of the container ( 2 ). Method according to claim 4, wherein at least one measurement on the sample ( 1 ) for obtaining information about the state of movement of the sample ( 1 ) takes place by means of an optical method. Method according to one of the preceding claims, wherein the sample ( 1 ) is a fluid medium or a liquid. Method according to claim 6, wherein the state of movement of the sample ( 1 ) a time- and location-dependent accumulation ( 3 ) of the fluid medium or the liquid in the container ( 2 ), and wherein the timing in step b is a position of accumulation ( 3 ) in the container ( 2 ) considered. Method according to one of the preceding claims, wherein the at least one variable is a concentration of a substance, a partial pressure of a gas, a pH or an absorbance or optical density of the sample ( 1 ). Method according to one of the preceding claims, wherein determining the at least one variable of the sample ( 1 ) comprises the following steps: sending an electromagnetic signal to the sample ( 1 ), • detecting an optical response from the container ( 2 ), and • determining the at least one variable from the optical response, wherein at least the emission of the electromagnetic signal with time by a by the movement of the container ( 2 ) resulting movement state of the sample ( 1 ). Method according to one of the preceding claims, wherein in the container ( 2 ) at least one sensor ( 4 ), which has an optical behavior dependent on the at least one variable. The method of claim 10, wherein the optical behavior is a luminescent phenomenon. Method according to one of the preceding claims, wherein the at least one variable is determined repeatedly. Contraption ( 20 ) for determining at least one variable in a moving container ( 2 ) ( 1 ), comprising: a support element ( 22 ) for the container ( 2 ), wherein with the support element ( 22 ) a defined movement is executable; a measuring system ( 21 ), by which at least one variable of the sample ( 1 ) is determinable; characterized by a synchronizing means ( 5 ), by which the determination of the at least one variable temporally with a by the movement of the container ( 2 ) can be generated motion state of the sample ( 1 ) is tunable. Contraption ( 20 ) according to claim 13, wherein the synchronizing means ( 5 ) a position measuring arrangement for measuring the position of the support element ( 22 ). Contraption ( 20 ) according to claim 13 or 14, wherein the synchronizing means ( 5 ) an acceleration sensor ( 51 ) for determining the acceleration of the support element ( 22 ). Contraption ( 20 ) according to claim 15, wherein the acceleration sensor ( 51 ) on the support element ( 22 ) is attached. Contraption ( 20 ) according to one of claims 13 to 16, wherein the synchronizing means ( 5 ) An institution ( 52 ) for obtaining information about the state of movement of the sample ( 1 ). Contraption ( 20 ) according to claim 17, wherein the device ( 52 ) comprises an optical system. Contraption ( 20 ) according to one of claims 13 to 18, wherein the measuring system ( 21 ) a controllable light source ( 6 ). Contraption ( 20 ) according to claim 19, wherein the light source ( 6 ) for cooperation with at least one in the container ( 2 ) mounted sensor ( 4 ) is trained. Contraption ( 20 ) according to claim 19 or 20, wherein the light source ( 6 ) Light from a plurality of wavelength ranges can be emitted. Contraption ( 20 ) according to one of claims 13 to 21, wherein the carrying element ( 22 ) for carrying a plurality of containers ( 2 ) is trained. Contraption ( 20 ) according to one of claims 13 to 22, wherein the support element ( 22 ) is a plate on which the at least one container ( 2 ) is fixable.

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