CS268239B1 - Attractive mixture for polyphagous synantrophous insects and method of its preparation - Google Patents

Abstract

The solution and concerns the attractive % I, consisting of weight percent 10 to 35% fish meal, 5 to 30% malt meal, 10-25% dried egg yolk, 1 to 6% palmitic acid, 1 to 5% liquorice extract and 1 to 5% aggregation or sex pheromone and longer to 30% rice flour, up to 15% dried milk, up to 15% of dried yeast, to 15% lactic acid, up to 5% maple aromatic, up to 1% 5, 9, 13-tr-methyl-4,6-tetradecadiene-3, 13-diol, up to 0.5% of an antioxidant and up to 0.2% of a preservative reagents. Preparation attractive Aaesl consists in being liquid recipes! the spit and a dissolve in the organic the ee solution obtained is dissolved he clung to the malt Scrap and after mixing the premix thus obtained is freed rozpuatldia, then gradually blend solids and after homogenization the resulting roe is compressed into tablets or tablets pellets, or left loose. The attractive ems of the invention were verified by field 1 laboratory teets at home, a warehouse worm and other pests, and commanded good results

Description

The present invention provides an atrophic composition for polyphagous synanthropic insects based on nutritionally rich materials and a process for its preparation.

Substances and mixtures of substances that attract insect pests such as cockroaches, crickets and other vaginal synanthropic species, for grinding, where they would be captured, killed or sterilized, are the basis of ethological oil-real methods. To date, a number of natural and synthetically prepared substances have been used as attractants or baits. mixes for rodents, various types of meat and dairy raw materials and products; various types of fruit and products from them, possible extracts of these products * Higher attractiveness was also found for chemically definable substances, eg * fatty acids, amino acids and additives, higher alcohols, sugars, norbornene derivatives and other cyclic compounds. The Council of these foodstuffs also attracts other pests and similar biology, such as crickets, ants, darkling beetles and others.

The disadvantages of the attractants used so far are the restriction of the scope only to certain population components, reduced effectiveness in habitats and a greater choice of competing incentives, such as food, moisture, fragmentation and usually effective only for a shorter distance. Natural foodstuffs dry out or decompose rapidly and, like volatile aynthotic substances, rapid inaktlvacl and sponsorship stimuli occur, often within 1-2 days. The difficulty is also the difficulty of standardizing the preparation and thus 1 industrial production. Finally, for a number of the listed simple substances, the demonstrable attractiveness was not confirmed in further tests.

The described shortcomings are eliminated by an attractive ama for polyphagous aynanthropic insects, the essence of which lies in the fact that it consists in weight percent of 10 to 35% fishmeal, 5 to 30% alad scrap, 10 to 25% ayonic egg yolk, 1 to 6% palmltic acid , 1 to 5% licorice extract, 1 to 5% aggregation or aexual pheromone and up to 30% rice flour, up to 15% sweet milk, up to 15% pounded yeast, up to 15% lactic acid, up to 5% maple aroma, up to 1 % 5,9,13-trimethyl-4,6-totradecadlon1,13-dlol, up to 0.5% antioxidant and up to 0.2% preservative.

The attractive method of preparation consists in dissolving the liquid formulations in an organic solvent, mixing the solution obtained with malt grist and, after mixing and removing the premix thus obtained, gradually mixing the solids and, after homogenization, compressing the resulting sculpture into tablets. , pallets, and ·, or leaves in the aypken state. A specific attribute of the attractive amal for the domestic ruaa (Blattella gernanlca), an aggregation pheromone, ae obtained from the culture medium of this species. The nymphs of the penultimate and last Instar are cultivated in horizontally positioned loading bottles under standard conditions for 2 weeks, after which the insects and feeders are removed and the remaining contents of the bottle, ie lining, oxuvia and true, are poured into a distillation apparatus into which water is introduced. which entrains volatile, biologically active components. The condensate in the cooling section is further shaken with a pad of diethyl ether and the ether fraction is separated. Other pheromones were identified and prepared aseptically.

The attractive mixture according to the invention consists, on the one hand, of dehydrated substances and higher nutrient levels, i.e. nutritionally rich and conditional on guaternal stimulation, namely fish meal, egg yolk, milk powder, rice flour, yeast and olfactory milk and palmlea kiaollny. To this baal are added substances that significantly increase the sensory attractiveness of the complete amSai, namely alad scrap, licorice extract, maple aroma and possibly 1 5,9,13-trimethyl-4,6-totradskadlen-3,13-dlol and a pheromone stimulating accretion. activity of the target species.

The attractive litter according to the invention has a high efficiency on both harmful species of cockroaches living in our country, i.e. domestic red cockroach (Blattella germanlca) and common cockroach (Blatta orlentolla), in all types of habitats,

CS 268 239 Bl 2 to determine. Modifications of mussels are also attractive for other synanthropic polyphagous pests, such as the domestic cricket / Gryllulus domeatlcua /, ants of the genera Monoaoriua and Laelue, beetles of the genera Tribolium, Tonebrioldee, Trogodarma, Stegoblua, etc. In terms of application methodology and wide use, mechanical or glue traps, or bait and the use of conventional log inoecticides or pesticides of future generations such as juvenolds, etheryllants, blopestlclds, etc. industrial production.

The high attractiveness and versatility of the media according to the invention were tested in a few laboratory field experiments.

The following are examples of the results of any of these biological comparative tests. Attractive emission flies were prepared in a starting wild of 1000 g.

Example 1. Attractive mixture for domestic ruea / Blattella geraanica / in the form of tab years / A /.

Sloleníi fishmeal, .100 g malt grist, Jeani ground 240g dried egg yolk 190g palmltic acid 9g licorice extract / CDK 758 491 2661 / 14g extract of agglomeration foromon 10g rice flour 270g dried milk 90g dried yeast crushed 50g

Maple aroma / ČOK 758 213 0185 / 20g

5,9,13-trimethyl-4,6-tetradecadiene-3,13-dlol 5g

2,6-dl-tert-butyl-4-ethyl phenol / antloxide / 1g sorbic acid / cons. reagent / 1g

A total of 1000g

The liquid formulations are dissolved with stirring in 100 ml of acetone, the solution obtained is added to finely ground malt grist, the mixture is mixed thoroughly and the acetone is removed on a rotary evaporator. The remaining formulation ingredients are gradually added to this premix, and the mixture is thoroughly homogenized and compressed with tablets weighing 1.5 g.

The field compared the teat attractiveness of the mixture according to invention A and food products on domestic ruau.

The mixture according to the invention (A) in the form of tablets was tested at 6 food establishments in comparison with liver pate, homogenized sardines, fresh white bread, ground walnut and ST1 feed mixture. The criterion of attractiveness was the number of captured Individuals during a 14-day exposure to glue traps of our own design with a centrally located 1.5 g of tidal material. 55 traps were used for each variant.

Table of results

At raktant Capture 0 per trap Coefficient Evidence / ke / attractiveness differences β AND 4213 76.6 ♦ 28.1 1.0 sardines 2721 50, '2 ♦ 17.5 . 0.66 0.02 White bread 1903 34.6 ♦ 11.3 0.45 0.01 STÍ 1464 26.6 ♦ 19.3 0.35 0.01 pate 1420 25.8 ♦ 8.4 0.34 0.01 Walnut 1298 23.6 ♦ 10.8 0.31 0.01

CS 268 239 Bl 3

Oak showed a test, which according to the invention proved to be more attractive to the species tested than the products classified as highly attractive. (many authors stated to be more attractive at all) and 3 or more times higher than in other variants * Differences in other tested, non-listed foods are even many times higher in favor of the mixture according to the invention *

Example 2 * Attractive «« 8 »for the dark blue / trlbollum confueum / in the form of granules / B /. ’

Ingredients: .

fish meal 233 G malt erot 350 G dried egg yolk 250 9 palmltic acid 50 G licorice extract 50 G 4,8-dimethyl decenal / egrog. foronom / 50 9 5,9,13-trimethyl-4,6-tert-radecadlon-3,13-diol 10 9 2,6-dl-tert-butyl-4-methyl phenol 5 9 aorbic acid 2 G Total looo G Method of preparing an attractive mixture for a darkling man warehouse yeah analogous to u

previous modification. After homogenization, granules weighing 0.3-0.5 g are compressed.

Laboratory comparative test of the attractiveness of ememei according to the invention B for the dark breeder.

With the circumference of a circular filter paper with a diameter of 15 cm placed in a Petri dish, 12 piles were evenly applied, alternating between the two tested alternatives. 100 individuals of the tested species were always placed in the vial. The mixture according to the invention (B) was always tested against the following known attractants

1 / mixture of white bread, peanuts, wheat and raisins)

2 / standard diet consisting of crushed wheat, oatmeal and dried yeast)

3 / milk powder

4 / malt scrap

5 / pellets STl / standard feed for rodents /

6 / pellets STl ♦ 0.5% 5,9,13-trimethyl-4,6-totradecadiene 3,13-dloluj

7 / egg yolk.

20% water was added to each substance to obtain a slightly dough consistency. At least 3 replications were performed for each variant. Sako attractiveness indicator was evaluated the total number of individuals collected in piles of individual variants / n «1) - 7) / during three readings, the 2nd day of exposure. The preference for variants 5) and 6) (last column of the table) was further determined in the subtest.

CS 26Θ 239 Bl

Table results Variant 1) 2) 3) 4) 5) 6) 7) Η B number to _ n 297 315 87 304 201 162 dli 132 363 pp 323 ϊδΏ 215 “Ή pref. ratio B / n 2.4 3.6 23.4 1.7 3.1 1.5 2.0 5.0 (M > 2 72.1 129.3 267.1 32.0 143.4 24.6 55.0 114.7 evidence P - 0.01 ♦ »♦ ♦ ♦ ♦ ♦ ♦ + T - T_ Π D 4 7 > ⁷ 3 > 7 4 4 -

The test has shown that the attractive substance according to the invention (0) is demonstrably / and not summatically / more attractive than its individual components 1 than the hitherto reported attractants, including the literature rated in the literature 1 / (Levinson, 1976). A partial test showed a significant increase in attractiveness upon delivery of 1% of 5,9,13-trimethyl-4,6-tetradecadiene-3,13-diol. The complete consumption of angel 0 occurred at least 3 days earlier than with the other variants.

- T - the difference between the time when the total consumption of the individual substances e araěal occurred

8.

Claims (3)

PRIORITY OF THE INVENTION 1. An attractive emitter for polyphagous synanthropic insects without nutritionally rich materials of pheronones, characterized in that it consists in% by weight of 10 to 35% of fishmeal, 5 to 30% of malt grist, 10 to 25% of dried egg yolk, 1 to 6% paleltic acid, 1 to 5% licorice extract and 1 to 5% aggregation or sex pheromone. 2. Attractive eaia according to point 1, characterized in that the other attractive constituents of amSal are up to 30% rice flour, up to 15% milk powder, up to 15% hardened yeast, up to 15% lactic acid, up to 5% maple flavor, up to 1 % Of 5,9,13-trimethyl-4,6-tetradocadlon-3,13-diol, up to 0.5% of antioxidant and up to 0.2% of preservative. 3. A process for the preparation of an attractive emission according to items 1 and 2, characterized in that the liquid formulation ee is dissolved in an organic solvent, the solution obtained is mixed with malt meal. after homogenization, the resulting amSa is pressed into slabs or pellets, or left in a bulk state.