CN87107897A - Be used to activate the composition of fermenting process and quicken the method for natural product fermentative degradation - Google Patents
Be used to activate the composition of fermenting process and quicken the method for natural product fermentative degradation Download PDFInfo
- Publication number
- CN87107897A CN87107897A CN198787107897A CN87107897A CN87107897A CN 87107897 A CN87107897 A CN 87107897A CN 198787107897 A CN198787107897 A CN 198787107897A CN 87107897 A CN87107897 A CN 87107897A CN 87107897 A CN87107897 A CN 87107897A
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- Prior art keywords
- saponin
- composition
- enzyme
- gram
- enzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/168—Steroids
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/14—Pretreatment of feeding-stuffs with enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Polymers & Plastics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Animal Husbandry (AREA)
- Physiology (AREA)
- General Chemical & Material Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Fodder In General (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to contain enzyme, particularly Glycosylase, cellulase, proteolytic enzyme, the composition class of esterase or amylase and saponin such as triterpene saponin, particularly soap bark saponin, activation fermentation process and acceleration natural product particularly contain the fermentative degradation of the polyose of xylogen.
Description
Fermenting process is by enzyme control, and enzyme itself produces by bioprocess.For example can respectively the glycosidic linkage in oligose and the polysaccharide be split unfolded Glycosylase is to produce by the Digestive tract of animal body with by microorganism.
Know already, in the animal-feed that is difficult to digest, add,, improved the usability of described animal-feed promptly with the Glycosylase, particularly cellulase of microbial process acquisition by biological approach.In addition, also know, the feed substrate that before feeding, has high cellulose content with cellulose treatment, be straw, sunflower seed extracts oil also or is like that, can reach on the one hand in the digestion animal stomach to be difficult to digest the Mierocrystalline cellulose that maybe can not digest, and also discharge nutritive substance on the other hand by above-mentioned digestion, as range protein, otherwise because cellulose chemistry and mechanical mixing, these nutritive substances can not be absorbed in the digestive process of animal.
The objective of the invention is to quicken this known self process, and improve its activity; Further aim of the present invention provides a kind of composition that generally can activate fermenting process, and said composition not only can digest the Mierocrystalline cellulose that is used to prepare animal-feed, and also can solve other different problems.
Natural prodcuts with high saponarin content are used as animal feed and have significant period of time. This respect is grain class and alfalfa fodder particularly, and this two Plants and plant product have respectively high saponarin content. The Saponaria officinalis glycoside itself is one group of compound with all a series of common properties. According to the chemical constitution of Saponaria officinalis glycoside, they are glycosides, and its aglycone is that sapogenin has different basic structure, are divided three classes triterpene Saponaria officinalis glycoside, neutral steroid Saponaria officinalis glycoside and alkaline steroid Saponaria officinalis glycoside according to the structure of saponarin. Then the economic worth of two classes is less, if entirely use it Words mainly are the raw materials that is used as some other materials of preparation such as pharmaceutical composition, because the surface-active of triterpene Saponaria officinalis glycoside, it can be employed technically. The soapbark extract that extracts from soapbark contains the triterpene saponarin, be can be used in the Food ﹠ Drink by U.S.'s food and drugs administration approved, and this extract is for the preparation of candy in the Orient.
In addition, the sweet class of Saponaria officinalis has been used for the farmland, as waste product inorganic agent (European patent EP-A10063621) and as the inorganic agent (EP-A10063622) of agricultural soil and forestry soil. In these two patents, add or use and contain the water-soluble of saponarin so that the growth of aerobic bacteria is better than the growth of anaerobic bacteria, a therefore side and can accelerate the waste product bacterial degradation, and also can accelerate on the other hand the bacterial degradation in soil. In addition, the growth that also proposes anaerobic bacteria in described method will be suppressed. In European patent EP-A1 that first is mentioned, also mention the Saponaria officinalis glycoside and can be used for processing the agricultural by product with high nutrient content. This class byproduct of mentioning has straw, particularly cornstalk or rice husk or like that, and this class agricultural by product can be made compost or add the Saponaria officinalis glycoside to be decomposed. Can obtain partly valuable animal feed by this decomposition. Decompose owing to the growth of bacterium in the case and the process of accelerated decomposition greatly. Because the sugared close existence as the aerobe nutrient media is indicated in the special decomposition of preparation animal feed, because sugared its close cohesive is so that saponarin can keep sticking to is difficult for above the solid removing.
Though can taking place one far and away, the method that is proposed quickens the putrid effect of nature by bacterium, but undertaken slowly by the method that bacterium causes, therefore mention as top, in order to quicken these natural processes, at first will separate by the slow organized enzyme that bacterial growth produced, use it for actual degradation process then, be used in particular for process by the animal-feed of agriculture waste product production.Owing in these processes, can directly use the enzyme of aequum in advance, thereby do not need by the time to form the required enzyme (if above-mentioned growth can be quickened to some extent) of requirement owing to the growth of bacterium chance, these processes of required enzyme have wherein been used, can directly carry out quickly basically more selectively and also.Therefore, in general, particularly from the economic point of view, waste material is produced animal-feed need select a kind of waste product such as straw of making, Sunflower shell (sunflowershot), and the method that chaff and so on can be degraded is so that can absorb the animal stomach better.
Once be surprised to find that the Saponaria officinalis glycoside, particularly triterpene Saponaria officinalis glycoside, especially soap bark saponin have and can quicken greatly by the degradation rate of the macromolecule product of enzyme liberating.Therefore can observe in the currently known methods with Mierocrystalline cellulose enzyme degraded cellulose, add saponin in degradation process, cellulosic Degradation Level is significantly improved.Other enzymes, as protease, the degradation rate of esterases also obtains similar result.
Therefore the present invention relates to a kind of composition for the activation sweat, said composition reaches arbitrarily diluent except comprising anticorrisive agent and stabilizing agent, colorant, and the fragrance conditioning agent beyond the thickener etc., also contains one or more enzymes and saponarin. As above carry, the Saponaria officinalis glycoside generally can be used for desired purpose, yet adopts the triterpene saponarin, preferably adopts the soapbark saponarin will obtain best result.
The used enzyme of the present invention refers in particular to glycosidase enzyme, preferably cellulase.Yet, use other glycosidase enzymes, for example also may play the beyond thought effect of accelerated degradation process with amylase.Other enzymes also can be used as protease or esterases.This particularly advantageous because in most of the cases the waste product of agricultural is not to be only limited to a certain predetermined substance, as Mierocrystalline cellulose, and contains other materials such as the protein or the fat that can be used as feed in this basal component.These other can be used as feed material since they mixed in the framework of cellulosic material, originally be not subjected to the influence of enzyme; Only after Mierocrystalline cellulose was by cellulose degraded, these other nutrient substances itself just might be decomposed or degrade owing to the effect of enzyme.
Typical composition of the present invention comprises for example every liter: 2 gram soap bark saponins, 500 gram enzymes, 5 gram sanitass and 300 gram tackiness agents, molasses are tackiness agents preferably.
If composition of the present invention need be preserved one long period, preferably enzyme and other composition branches are come and deposit, avoiding unwanted reaction in mixture, and these reactions can weaken expected effect.
The present invention relates to a method of quickening the natural product fermentative degradation in addition, wherein enzyme is added in the natural product, particularly have in the polyose of high lignin content, this method comprises adds saponin or saponin aglucon respectively or adds extract or the preparation that contains saponin or saponin aglucon respectively, and its amount contains the saponin or the sapogenin of 0.2 to 20 gram respectively with matrix per ton.
In this method, also use triterpene Saponaria officinalis glycoside, particularly soap bark saponin, but preferably use with the form of soap bark extract.
As mentioned above, cellulase in the method for the invention, amylase, proteolytic enzyme and esterase also can be used alone or as a mixture.
Because the enzyme expensive price, and begin used a certain amount of enzyme and can not show the booster action that landed, so the consideration economically of the main basis of the enzyme-added upper limit decides.
Method of the present invention can reality be implemented under each temperature that fermenting process carries out, because fermenting process can more promptly carry out when temperature is raised to a specified temp, therefore exists a preferred temperature.
Therefore preferably at first determine the amount of saponin by tentative experiment of short duration, the optimal conditionss such as temperature of enzyme amount and process just carry out method of the present invention with a large amount of matrix then.
The method according to this invention might be transformed into the animal-feed with high value with waste product first and not need with complicated method.Because the method according to this invention, unnecessary culturing bacterium, only need feeding for the previous period or the feeding forward direction matrix in sight mixture of adding enzyme and saponin just can.The previous case depends on adds the time length of composition to the feeding, matrix can partly be decomposed or all be decomposed, latter event, promptly want shortly before the feeding mixture of saponin and enzyme to be added in the feed, mixture will play digestive aid and the matrix that takes place in animal digestive system is decomposed to be improved.Clearly, the latter event place that can save time and store green fodder particularly.Because up to the present-particularly situation-these products of decomposing waste prods with bacteria culture medium segment length's time of must storing in one special jar is degraded respectively and decomposes waste prods until the enough enzyme of added bacterium generation.
In addition, the compositions and methods of the invention also can be used for other aspects except that the decomposition natural product are made the animal feed.Generally speaking the compositions and methods of the invention can be used for the situation that all will be split into highly condensed natural products less chemical unit, as verified, if be favourable with seed through compositions-treated of the present invention prior to seeding, because high molecular seed hulls can be degraded at least partially, so just promoted germinating power greatly.
The present invention can illustrate by following Example, but be not limited to this.
Example: handle Sunflower Receptacle with the present composition that contains cellulase and extract shell (sunflower extraction shot).
Test in the laboratory, wherein handle Sunflower Receptacle and extract shell with composition of the present invention, and respectively separately with cellulase with handle Sunflower Receptacle extraction shell to compare with saponin separately.
From all samples, prepare its aqueous solution, and measure increment, after 48 hours various samples are carried out the first time at the processing slag charge and analyze,, analyzed every two months at last then every 1 month from the glucose that lignocellulose generated.After during this, the slag of handling added (common standard feed) in several animal-feeds and be 30 to 60 kilograms piggy and big porker with the forage feed body weight of being handled.
By this feeding study, will with the analytical results that the laboratory obtains attached in practice, the slag charge amount in the feed added on the one hand can increase (because the digestibility of difference and fatten characteristic, add undressed slag charge amount at present and mostly be most 3%), on the other hand, although owing to increased the body weight that treated hundred umbers of crossing the stain material have still increased pig greatly.
1) contrast: unprocessed slag charge glucose %(weight)
After 48 hours 0.028
No change still after 2 months
2) use cellulase 300 international unit/milligram raw fibre separately
After 48 hours 0.075%
After January 0.078%
After February 0.194%
3) use soap bark saponin solution/ton of 500ml 2% separately
After 48 hours 0.100%
No change still after 2 months
4) cellulase of soap bark saponin solution+above-mentioned dosage
After 48 hours 0.081%
After January 0.196%
After February 0.608%
Feeding experiment:
1) piggy
A) with the control feed that does not contain the Sunflower Receptacle slag charge
B) with containing the with good grounds the above-mentioned the 4th) test feed of the slag charge handled
Every day, the increase of body weight was calculated a) 390 gram b with gram) 410 grams
Consume a) 3.54 kilograms of b of feed kilogram number/increase per kilogram of body weight) 3.51 kilograms
2) piggy (body weight is between 30 and 60 kilograms)
A) with containing 3%(weight) control feed of undressed slag
B) with containing 10%(weight) according to the above-mentioned the 4th) handled the test feed of slag charge
Every day, the increase of body weight was calculated a) 696 gram b with gram) 804 grams
Consume a) 3.91 kilograms of b of feed kilogram number/production per kilogram meat) 2.54 kilograms
3) big porker fattens at last
A) with containing 3%(weight) control feed of unprocessed slag charge
B) with containing 12%(weight) according to the above-mentioned the 4th) handled the test feed of slag charge
Every day, weight increase was calculated a) 593 gram b with gram) 638 grams
Consume a) 4.82 gram b of feed kilogram number/production per kilogram meat) 4.37 grams
Experimental animal head number: 240
Because through compositions-treated of the present invention, the consumption that extracts shell as the Sunflower Receptacle of protein carrier can increase widely, expensive soybean import then can reduce.
Though the present invention clearly, can make changes and improvements without departing from the spirit and scope of the present invention through being described in detail and with object lesson.
Claims (12)
1, a kind of composition that is used to activate fermenting process reaches thinner arbitrarily comprising removing sanitas and stablizer, colorant, fragrance conditioning agent, thickening material and one or more enzymes and the saponin of also containing in addition like that.
2, the composition of claim 1, wherein saponin is a kind of triterpene saponin.
3, the composition of claim 2, wherein saponin is the soap bark saponin.
4, the composition one of in the claim 1 to 3 can comprise Glycosylase separately, cellulase, proteolytic enzyme, esterase or amylase or be in the same place with other enzymes.
5, the composition of one of claim 1 to 4, its every liter comprises 2 gram soap bark saponins, 500 gram enzymes, 5 gram sanitass and 300 gram tackiness agents.
6, a kind of method of quickening the natural product fermentative degradation, wherein enzyme is added in the xylogen that natural product particularly contain polyose, this method comprises respectively to be added saponin and sapogenin or the extract or the preparation that contain saponin or sapogenin respectively in the matrix with the amount that matrix per ton contains 0.2 to 20 gram saponin or sapogenin respectively.
7, the method for claim 6 is wherein used the triterpene saponin.
8, the method for claim 7 is wherein used the soap bark saponin.
9, the method for one of claim 6 to 8, wherein cellulase can be used as the enzyme use.
10, the method for one of claim 6 to 8 wherein amylase can be used as enzyme and use.
11, the method for one of claim 6 to 8 amylase wherein, the mixture of proteolytic enzyme and esterase can be used as enzyme and uses.
12, use remove sanitas and stablizer and thinner arbitrarily a kind of comprising, colorant, the fragrance conditioning agent, thickening material and the outer composition that also contains one or more enzymes like that activate fermenting process.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ATA3054/86 | 1986-11-17 | ||
| AT0305486A AT386838B (en) | 1986-11-17 | 1986-11-17 | MEANS FOR ACTIVATING FERMENTATIVE PROCESSES AND METHOD FOR ACCELERATING THE FERMENTATIVE DEGRADATION OF NATURAL SUBSTANCES |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN87107897A true CN87107897A (en) | 1988-05-25 |
Family
ID=3544587
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN198787107897A Pending CN87107897A (en) | 1986-11-17 | 1987-11-16 | Be used to activate the composition of fermenting process and quicken the method for natural product fermentative degradation |
Country Status (10)
| Country | Link |
|---|---|
| EP (1) | EP0268575A3 (en) |
| CN (1) | CN87107897A (en) |
| AT (1) | AT386838B (en) |
| DD (1) | DD262876A5 (en) |
| EG (1) | EG18005A (en) |
| HU (1) | HU204302B (en) |
| SU (1) | SU1679962A3 (en) |
| TR (1) | TR23485A (en) |
| YU (1) | YU206387A (en) |
| ZA (1) | ZA878090B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1034388C (en) * | 1991-06-13 | 1997-04-02 | 欧阳华 | Process for preparing forage from tobacco dregs |
| CN1048181C (en) * | 1993-10-21 | 2000-01-12 | 谭礼 | Process for producing biochemical raw material of spore powder of glossy ganoderma |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU5350590A (en) * | 1989-03-28 | 1990-10-22 | Peter Lenauer | Additive for food and fodder |
| DE4139871C2 (en) * | 1991-11-29 | 1996-05-23 | Hilgenfeld Thomas | Feed mixture for trout breeding |
| GB2309036A (en) | 1996-01-11 | 1997-07-16 | Garth M Henderson | Saponin and carbohydrate promote bio-degradation of organic waste |
| CN1165628A (en) * | 1996-02-14 | 1997-11-26 | 浙江农业大学 | Triterpenoid saponin extracted from tea oil cake and application as additive |
| DK174176B1 (en) * | 2000-12-27 | 2002-08-12 | Nor Feed Aps | Use of quillaja powder for the preparation of an oral agent to ensure milk quality |
| CN1212072C (en) * | 2002-12-23 | 2005-07-27 | 北京市食品研究所 | Activation of hydrolase for proteins in seeds of oil crops and hydrolysis for vegetable protein |
| US7604967B2 (en) * | 2003-03-19 | 2009-10-20 | The Trustees Of Dartmouth College | Lignin-blocking treatment of biomass and uses thereof |
| US8580541B2 (en) | 2003-03-19 | 2013-11-12 | The Trustees Of Dartmouth College | Lignin blockers and uses thereof |
| CA2839794A1 (en) | 2011-05-20 | 2012-11-29 | Advandx, Inc. | Selective ultrasonic lysis of blood and other biological fluids and tissues |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB893417A (en) * | 1959-01-08 | 1962-04-11 | Pfizer & Co C | Growth stimulating animal feed composition |
| BE670523A (en) * | 1964-10-09 | |||
| ATE14417T1 (en) * | 1981-04-24 | 1985-08-15 | Schmittmann Sk Pharma | TREATMENT AGENT FOR WASTE PRODUCTS. |
| US4557927A (en) * | 1983-03-10 | 1985-12-10 | Kabushiki Kaisha Hoyashibara | Food products and process for producing same |
-
1986
- 1986-11-17 AT AT0305486A patent/AT386838B/en not_active IP Right Cessation
- 1986-11-26 HU HU864886A patent/HU204302B/en not_active IP Right Cessation
- 1986-12-01 EG EG745/86A patent/EG18005A/en active
-
1987
- 1987-10-28 ZA ZA878090A patent/ZA878090B/en unknown
- 1987-11-11 EP EP87890253A patent/EP0268575A3/en not_active Withdrawn
- 1987-11-13 DD DD87309021A patent/DD262876A5/en not_active IP Right Cessation
- 1987-11-13 TR TR799/87A patent/TR23485A/en unknown
- 1987-11-13 YU YU02063/87A patent/YU206387A/en unknown
- 1987-11-16 CN CN198787107897A patent/CN87107897A/en active Pending
- 1987-11-16 SU SU874203709A patent/SU1679962A3/en active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1034388C (en) * | 1991-06-13 | 1997-04-02 | 欧阳华 | Process for preparing forage from tobacco dregs |
| CN1048181C (en) * | 1993-10-21 | 2000-01-12 | 谭礼 | Process for producing biochemical raw material of spore powder of glossy ganoderma |
Also Published As
| Publication number | Publication date |
|---|---|
| EP0268575A2 (en) | 1988-05-25 |
| DD262876A5 (en) | 1988-12-14 |
| ATA305486A (en) | 1988-03-15 |
| HUT45556A (en) | 1988-07-28 |
| TR23485A (en) | 1990-01-31 |
| EP0268575A3 (en) | 1989-07-19 |
| AT386838B (en) | 1988-10-25 |
| YU206387A (en) | 1988-10-31 |
| HU204302B (en) | 1991-12-30 |
| SU1679962A3 (en) | 1991-09-23 |
| ZA878090B (en) | 1988-10-26 |
| EG18005A (en) | 1991-08-30 |
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| PB01 | Publication | ||
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| SE01 | Entry into force of request for substantive examination | ||
| C01 | Deemed withdrawal of patent application (patent law 1993) | ||
| WD01 | Invention patent application deemed withdrawn after publication |