CN2609931Y - Magnetic cell separator - Google Patents
Magnetic cell separator Download PDFInfo
- Publication number
- CN2609931Y CN2609931Y CN 03230095 CN03230095U CN2609931Y CN 2609931 Y CN2609931 Y CN 2609931Y CN 03230095 CN03230095 CN 03230095 CN 03230095 U CN03230095 U CN 03230095U CN 2609931 Y CN2609931 Y CN 2609931Y
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- CN
- China
- Prior art keywords
- magnetic
- cylinder body
- cell
- cylindrical shell
- cell separator
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 230000005291 magnetic effect Effects 0.000 title claims abstract description 39
- 239000011324 bead Substances 0.000 claims abstract description 39
- 230000007423 decrease Effects 0.000 claims description 3
- 238000000926 separation method Methods 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 3
- 230000005426 magnetic field effect Effects 0.000 abstract 1
- 239000000203 mixture Substances 0.000 description 7
- 238000000034 method Methods 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- BGPVFRJUHWVFKM-UHFFFAOYSA-N N1=C2C=CC=CC2=[N+]([O-])C1(CC1)CCC21N=C1C=CC=CC1=[N+]2[O-] Chemical compound N1=C2C=CC=CC2=[N+]([O-])C1(CC1)CCC21N=C1C=CC=CC1=[N+]2[O-] BGPVFRJUHWVFKM-UHFFFAOYSA-N 0.000 description 2
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000006249 magnetic particle Substances 0.000 description 1
- 238000007885 magnetic separation Methods 0.000 description 1
- 239000006148 magnetic separator Substances 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
A magnetic cell separator comprises a cylinder body (1) with openings at the upper end and the lower end and magnetic bead (3) groups arranged in the cylinder body, wherein the cylinder body (1) is divided into three parts (11, 12 and 13) from top to bottom, the inner diameters of the three parts are sequentially reduced, and the magnetic bead (3) groups are filled in the middle part (12) of the cylinder body; the magnetic conductive beads (3) preferably have a diameter of 50-1500 microns. The magnetic cell separator can also comprise a piston rod (2) which can be inserted into the upper part (11) of the cylinder body and can do piston motion relative to the cylinder body; the periphery of the lower part (13) of the cylinder body can be also provided with an annular convex rib (13 a). Compared with the prior art, the utility model has the advantages of through filling the great magnetic conduction pearl of relative immunomagnetic bead of diameter in the barrel, can adsorb the immunomagnetic bead on the magnetic conduction pearl together with the cell by the mark under external magnetic field effect effectively to guarantee effectively going on of separation, owing to the existence in clearance between the less magnetic conduction pearl of relative barrel diameter again, avoided the immunomagnetic bead together with by the collision between the mark cell effectively, thereby guarantee the activity of cell.
Description
Technical field
The utility model relates to cell and other biological sample separation technical field, particularly immunomagnetic beads method cell separation technology field, particularly, is a kind ofly to be used to separate by the magnetic separator of the cell of immunomagnetic beads mark.
Background technology
The immunomagnetic beads method cell separation technology is a kind of very simple and direct, biomaterial separates completely, method qualitatively, it is widely used in cytobiology, immunology, therapeutics, field such as diagnostics and pharmacy, its principle is " utilizing the magnetic-particle of pan coating the to come isolated cell " imagination that is proposed according to Dane Arcmbaum and R.S Yen, in nanometer to micron order, Sheet clonal antibody on the good microballon of certain magnetic and dispersity (is ZL99252058.4 " immunomagnetic beads " as the patent No.) is arranged, carrying out specificity by this monoclonal antibody with the antigen on cell (or other materials) surface combines and it is marked on the immunomagnetic beads, by magnetic separation technique immunomagnetic beads is separated from original mixture together with the cell that is labeled (or other materials) again, usually, be by a conduit with cell mixture, one magnet is set outside conduit, immunomagnetic beads is adsorbed on the tube wall under the magnetic effect together with the cell that is labeled, other materials then unhinderedly pass through this zone, thereby reach isolating purpose.Yet there is following shortcoming in this isolation technique: 1. under external magnet effect, immunomagnetic beads moves along field direction rapidly together with the cell that is labeled, and causes mutual collision easily, and immunomagnetic beads might enter or pass cell, thereby causes the damage of cell; 2. for the nano level immunomagnetic beads, because it is undersized, because of being difficult to be attracted and to separate by external magnetic field.
Summary of the invention
Technical problem to be solved in the utility model is to provide a kind of magnetic cell separator simple in structure, easy to use at above-mentioned prior art present situation.
The utility model solves the problems of the technologies described above the technical scheme that is adopted: this magnetic cell separator comprises the cylindrical shell of both ends open about in the of one and its interior magnetic conductive bead group is set, cylindrical shell is divided into three parts from top to bottom, and internal diameter successively decreases successively, and described magnetic conductive bead group promptly is filled in the middle part of cylindrical shell.
Described magnetic conductive bead is good with 50～1500 microns of diameters.
During use, at medium position one external magnet is set near cylindrical shell, then cell mixture to be separated is poured into from the top of cylindrical shell, mixture relies on its deadweight dirty gradually, and the lower part outlet from cylindrical shell after the magnetic bead group flows out, at this moment, wherein immunomagnetic beads has been attracted on the magnetic conductive bead group together with the cell that is labeled, and removes magnet then, at this moment, be by the cell of immunomagnetic beads mark from the lower part outlet of cylindrical shell is effusive, thereby realize isolating purpose.
Under the situation less by the content of the cell of immunomagnetic beads mark in mixture, in order to accelerate to be flowed out by the lower part outlet of the cell of immunomagnetic beads mark from cylindrical shell, described magnetic cell separator also can include one and can insert cylindrical shell top and can it make the piston rod of piston action relatively.
Needing material to import on specific vessel or the correlation detection instrument under some occasion through conduit with after separating, so that the operation of downstream work, can be outside the cylindrical shell bottom, at this moment with the direct sleeve of associated catheter, can be provided with annular convex rib in the periphery of cylindrical shell bottom, so that more firm grafting to be provided.
Compared with prior art, advantage of the present utility model is by the bigger magnetic conductive bead of filling premunition magnetic bead diameter in cylindrical shell, can under external the action of a magnetic field, immunomagnetic beads be adsorbed on the magnetic conductive bead together with the cell that is labeled effectively, thereby guarantee isolatingly effectively to carry out, again because the relatively existence in gap between the less magnetic conductive bead of barrel diameter, avoid immunomagnetic beads together with the collision that is labeled between the cell effectively, thereby guaranteed cell activity.
Description of drawings
Fig. 1 is the structural representation of the utility model embodiment.
Fig. 2 is the partial enlarged drawing of Fig. 1.
Fig. 3 is the partial enlarged drawing of the utility model embodiment when using by external magnetic patch.
Embodiment
Embodiment describes in further detail the utility model below in conjunction with accompanying drawing.
As shown in Figure 1 and Figure 2, the magnetic cell separator comprises that the cylindrical shell 1 of both ends open about in the of is divided into three parts 11,12,13 from top to bottom, and internal diameter successively decreases successively, the top 11 of cylindrical shell is inserted with one can it make the piston rod 2 of piston action relatively, the middle part 12 of cylindrical shell is full of magnetic conductive bead 3, and the periphery of cylindrical shell bottom 13 is provided with annular convex rib 13a, so that more firm grafting is provided with the direct sleeve of conduit the time.
During use, as shown in Figure 3, in 12 positions, middle part one external magnet 5 is set near cylindrical shell, extract piston rod 2 then, and cell mixture to be separated poured into from the top 11 of cylindrical shell, mixture relies on its deadweight dirty gradually, through the gap of magnetic conductive bead 3, flow out from bottom 13 outlets of cylindrical shell at last, at this moment, wherein immunomagnetic beads has been attracted on the magnetic conductive bead 3 together with the cell that is labeled, remove magnet 5 then, piston rod 2 is inserted the top 11 of cylindrical shell, and push away downwards, this moment, the bottom 13 from cylindrical shell exported effusive being by the cell of immunomagnetic beads mark, thereby realized isolating purpose.
Claims (5)
1, a kind of magnetic cell separator, the magnetic conductive bead (3) that it is characterized in that comprising the cylindrical shell (1) of both ends open about in the of one and be provided with in it is organized, cylindrical shell (1) is divided into three parts (11,12,13) from top to bottom, and internal diameter successively decreases successively, and described magnetic conductive bead (3) group promptly is filled in the middle part (12) of cylindrical shell.
2, magnetic cell separator according to claim 1 is characterized in that described magnetic conductive bead (3) diameter is 50～1500 microns.
3, magnetic cell separator according to claim 1 and 2 is characterized in that also including one and can insert cylindrical shell top (11) and can it make the piston rod (2) of piston action relatively.
4, magnetic cell separator according to claim 1 and 2 is characterized in that the periphery of described cylindrical shell bottom (13) is provided with annular convex rib (13a).
5, magnetic cell separator according to claim 3 is characterized in that the periphery of described cylindrical shell bottom (13) is provided with annular convex rib (13a).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03230095 CN2609931Y (en) | 2003-04-05 | 2003-04-05 | Magnetic cell separator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03230095 CN2609931Y (en) | 2003-04-05 | 2003-04-05 | Magnetic cell separator |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2609931Y true CN2609931Y (en) | 2004-04-07 |
Family
ID=34165175
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 03230095 Expired - Fee Related CN2609931Y (en) | 2003-04-05 | 2003-04-05 | Magnetic cell separator |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2609931Y (en) |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101921706A (en) * | 2009-06-15 | 2010-12-22 | 王全兴 | Magnetic cell separator |
| CN101939645A (en) * | 2007-12-05 | 2011-01-05 | 齐翁米克斯股份有限公司 | Cell assay kit and method |
| CN101482462B (en) * | 2009-02-18 | 2011-09-28 | 长春光机医疗仪器有限公司 | Nano-bead and liquid separation device of full-automatic illumination instrument |
| US8701893B2 (en) | 2009-12-23 | 2014-04-22 | Industrial Technology Research Institute | Magnetic separation device and method for separating magnetic substance in bio-samples |
| US8993342B2 (en) | 2011-04-11 | 2015-03-31 | Industrial Technology Research Institute | Magnetic separation unit, magnetic separation device and method for separating magnetic substance in bio-samples |
| CN107907691A (en) * | 2017-11-15 | 2018-04-13 | 南通伊仕生物技术股份有限公司 | A kind of myoglobin assay kit and its application method |
| CN107918022A (en) * | 2017-11-17 | 2018-04-17 | 南通伊仕生物技术股份有限公司 | A kind of cTnI detection kits and its application method |
| CN107942051A (en) * | 2017-11-15 | 2018-04-20 | 南通伊仕生物技术股份有限公司 | A kind of D dimer detection kits and its application method |
| CN107957495A (en) * | 2017-11-17 | 2018-04-24 | 南通伊仕生物技术股份有限公司 | A kind of CK-MB detection kits and its application method |
| US10625272B2 (en) | 2015-11-18 | 2020-04-21 | Industrial Technology Research Institute | Magnetic separator |
-
2003
- 2003-04-05 CN CN 03230095 patent/CN2609931Y/en not_active Expired - Fee Related
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101939645A (en) * | 2007-12-05 | 2011-01-05 | 齐翁米克斯股份有限公司 | Cell assay kit and method |
| CN101939645B (en) * | 2007-12-05 | 2014-07-30 | 齐翁米克斯股份有限公司 | Cell assay kit and method |
| CN101482462B (en) * | 2009-02-18 | 2011-09-28 | 长春光机医疗仪器有限公司 | Nano-bead and liquid separation device of full-automatic illumination instrument |
| CN101921706A (en) * | 2009-06-15 | 2010-12-22 | 王全兴 | Magnetic cell separator |
| US8701893B2 (en) | 2009-12-23 | 2014-04-22 | Industrial Technology Research Institute | Magnetic separation device and method for separating magnetic substance in bio-samples |
| US8993342B2 (en) | 2011-04-11 | 2015-03-31 | Industrial Technology Research Institute | Magnetic separation unit, magnetic separation device and method for separating magnetic substance in bio-samples |
| US10625272B2 (en) | 2015-11-18 | 2020-04-21 | Industrial Technology Research Institute | Magnetic separator |
| CN107907691A (en) * | 2017-11-15 | 2018-04-13 | 南通伊仕生物技术股份有限公司 | A kind of myoglobin assay kit and its application method |
| CN107942051A (en) * | 2017-11-15 | 2018-04-20 | 南通伊仕生物技术股份有限公司 | A kind of D dimer detection kits and its application method |
| CN107907691B (en) * | 2017-11-15 | 2020-10-30 | 南通伊仕生物技术股份有限公司 | Myoglobin detection kit and use method thereof |
| CN107942051B (en) * | 2017-11-15 | 2021-02-26 | 南通伊仕生物技术股份有限公司 | D-dimer detection kit and use method thereof |
| CN107957495A (en) * | 2017-11-17 | 2018-04-24 | 南通伊仕生物技术股份有限公司 | A kind of CK-MB detection kits and its application method |
| CN107918022A (en) * | 2017-11-17 | 2018-04-17 | 南通伊仕生物技术股份有限公司 | A kind of cTnI detection kits and its application method |
| CN107918022B (en) * | 2017-11-17 | 2020-10-09 | 南通伊仕生物技术股份有限公司 | cTnI detection kit and use method thereof |
| CN107957495B (en) * | 2017-11-17 | 2020-10-09 | 南通伊仕生物技术股份有限公司 | CK-MB detection kit and using method thereof |
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| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |