CN220626198U - Dual wavelength ultraviolet detector - Google Patents
Dual wavelength ultraviolet detector Download PDFInfo
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- CN220626198U CN220626198U CN202322025221.1U CN202322025221U CN220626198U CN 220626198 U CN220626198 U CN 220626198U CN 202322025221 U CN202322025221 U CN 202322025221U CN 220626198 U CN220626198 U CN 220626198U
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- 230000009977 dual effect Effects 0.000 title claims abstract description 23
- 238000005070 sampling Methods 0.000 claims abstract description 20
- 210000005056 cell body Anatomy 0.000 claims abstract description 15
- 230000003287 optical effect Effects 0.000 claims description 39
- 210000004027 cell Anatomy 0.000 claims description 4
- 238000001514 detection method Methods 0.000 abstract description 11
- 239000000126 substance Substances 0.000 description 6
- 238000002835 absorbance Methods 0.000 description 2
- 238000003384 imaging method Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000010586 diagram Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000003071 parasitic effect Effects 0.000 description 1
- 238000001742 protein purification Methods 0.000 description 1
- 238000000825 ultraviolet detection Methods 0.000 description 1
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Abstract
The disclosure relates to a dual wavelength ultraviolet detector, comprising a first light path component, a second light path component, a light splitting sheet, a flow cell body, a sampling photocell and a reference photocell; the first light path component can emit a first light beam with a first wavelength and parallel to the first light beam, and the second light path component can emit a second light beam with a second wavelength and parallel to the first light beam; the beam splitter can reflect part of the first light beam to sequentially pass through the first observation port, the solution in the flow channel and the second observation port along the second direction so as to shoot to the sampling photocell; the beam splitter can also allow another portion of the first light beam to be transmitted toward the reference photocell; the beam splitter can transmit part of the second light beam to sequentially pass through the first observation port, the solution in the flow channel and the second observation port along the second direction so as to irradiate the sampling photocell; the beamsplitter can also allow another portion of the second beam to reflect toward the reference photocell. The dual-wavelength ultraviolet detector can effectively shorten the error in detection time and improve the detection precision.
Description
Technical Field
The present disclosure relates to the field of ultraviolet detection technology, and in particular, to a dual wavelength ultraviolet detector.
Background
In the protein purification detection experiment, two single-light-path chromatographic instruments are often adopted for detection, one single-light-path chromatographic instrument is used for detecting DNA, the other single-light-path chromatographic instrument is used for detecting protein, and a solution to be detected needs to flow from a flow cell in one single-light-path chromatographic instrument to a flow cell of the other single-light-path chromatographic instrument, so that the error in detection time is larger, and the detection accuracy is influenced.
Disclosure of Invention
It is an object of the present disclosure to provide a dual wavelength ultraviolet detector capable of solving technical problems existing in the related art.
In order to achieve the above object, the present disclosure provides a dual wavelength ultraviolet detector including a first optical path assembly, a second optical path assembly, a light splitting sheet, a flow cell body, a sampling photocell, and a reference photocell; the first light path component can emit a first light beam with a first wavelength and parallel, and the second light path component can emit a second light beam with a second wavelength and parallel;
the flow cell comprises a flow cell body, wherein a flow channel for flowing solution and extending along a first direction is formed in the flow cell body, a first observation port and a second observation port are formed in the flow cell body, the first observation port and the second observation port are oppositely arranged along a second direction and are both used for being oppositely arranged with the flow channel, the light splitting sheet is arranged at the first observation port, and the sampling photocell is arranged at the second observation port;
the light splitting sheet can reflect part of the first light beam to sequentially pass through the first observation port, the solution in the flow channel and the second observation port along the second direction so as to irradiate the sampling photocell; the beam splitter sheet is further capable of allowing another portion of the first light beam to be transmitted toward the reference photocell;
the light splitting sheet can transmit part of the second light beam to sequentially pass through the first observation port, the solution in the flow channel and the second observation port along the second direction so as to irradiate the sampling photocell; the beam splitter sheet is further capable of allowing another portion of the second light beam to reflect toward the reference photocell; wherein the first direction and the second direction are perpendicular to each other.
Optionally, the first light path component includes a first light source emitter capable of emitting light rays of a first wavelength and a first collimating lens disposed on an emitting side of the first light source emitter for collimating the light rays of the first wavelength into a first light beam of a parallel light beam; and/or the number of the groups of groups,
the second light path component comprises a second light source emitter and a second collimating lens, the second light source emitter can emit light rays with a second wavelength, and the second collimating lens is arranged on the light emitting side of the second light source emitter and is used for collimating the light rays with the second wavelength into a second light beam of parallel light beams.
Optionally, the first light path component further includes a first optical filter disposed between the first light source emitter and the first collimating lens; and/or the number of the groups of groups,
the second light path component further comprises a second optical filter, and the second optical filter is arranged between the second light source emitter and the second collimating lens.
Optionally, the first light path component further includes a first diaphragm, the first diaphragm being disposed between the first light source emitter and the first collimating lens; and/or the number of the groups of groups,
the second light path assembly further comprises a second diaphragm, and the second diaphragm is arranged between the second light source emitter and the second collimating lens.
Optionally, the first light path component further includes a first mounting member, where the first light source emitter, the first diaphragm, the first optical filter, and the first collimating lens are sequentially disposed at intervals in the first mounting member along the light path direction of the first light path component; and/or the number of the groups of groups,
the second light path component further comprises a second mounting piece, and the second light source emitter, the second diaphragm, the second optical filter and the second collimating lens are sequentially arranged on the second mounting piece at intervals along the light path direction of the second light path component.
Optionally, the dual wavelength ultraviolet detector further comprises a controller, the first light source emitter and the second light source emitter are each electrically connected to the controller, and the controller is operable to: the first light source emitter and the second light source emitter are controlled to periodically and alternately emit light.
Optionally, the beam splitter extends along the first direction, the incident angle and the reflection angle of the first light beam on the beam splitter are both 45 °, and the incident angle and the reflection angle of the second light beam on the beam splitter are both 45 °.
Optionally, the dual wavelength ultraviolet detector further includes a first observation mirror and a second observation mirror, the first observation mirror is disposed at the first observation port, and the second observation mirror is disposed at the second observation port.
Optionally, the optical axis of the first optical path component is perpendicular to the optical axis of the second optical path component.
Optionally, the first wavelength is 260nm and the second wavelength is 280nm.
In the above technical scheme, through setting up first light path subassembly and second light path subassembly, first light path subassembly can jet out first light beam of first wavelength and parallel, and second light path subassembly can jet out second light beam of second wavelength and parallel. A portion of the first light beam is reflected by the beam splitter sheet through the solution in the flow channel to be directed to the sample photocell, and another portion of the first light beam is transmitted by the beam splitter sheet to be directed to the reference photocell. A part of the light rays of the second light beam penetrate through the light splitting sheet and then pass through the solution in the flow channel to be emitted to the photocell, and the other part of the light rays of the second light beam are reflected by the light splitting sheet and then emitted to the reference photocell. Because the wavelengths of the first light beam and the second light beam are different, energy losses of different degrees can be generated after the light beams pass through the solution, and according to the beer-lambert law (when a beam of parallel monochromatic light vertically passes through a uniform non-scattering light-absorbing substance, the absorbance of the parallel monochromatic light is proportional to the concentration of the light-absorbing substance), the concentration of two different substances in the solution can be detected by comparing a sampling photocell with a reference photocell. When detecting, the first light path component and the second light path component can emit light beams in a rapid and alternative mode, so that errors in detection time can be shortened, and detection accuracy is improved.
Additional features and advantages of the present disclosure will be set forth in the detailed description which follows.
Drawings
The accompanying drawings are included to provide a further understanding of the disclosure, and are incorporated in and constitute a part of this specification, illustrate the disclosure and together with the description serve to explain, but do not limit the disclosure. In the drawings:
fig. 1 is a schematic diagram of a dual wavelength ultraviolet detector according to one embodiment of the present disclosure.
Fig. 2 and 3 are schematic structural views of a flow cell body of a dual wavelength ultraviolet detector according to an embodiment of the present disclosure.
Fig. 4 is a cross-sectional view of a flow cell body of a dual wavelength ultraviolet detector of one embodiment of the present disclosure.
Description of the reference numerals
1. First light path component 11 first light source emitter
12. First collimating lens 13 first filter
14. Second optical path component of first diaphragm 2
21. Second light source emitter 22 second collimating lens
23. Second filter 24 second diaphragm
3. Light splitting sheet 4 flow cell body
41. First viewing port 410 first viewing mirror
42. Second viewing port 420 second viewing mirror
5. Sampling photocell 6 reference photocell
A first direction B second direction
40. Flow passage
Detailed Description
Specific embodiments of the present disclosure are described in detail below with reference to the accompanying drawings. It should be understood that the detailed description and specific examples, while indicating and illustrating the disclosure, are not intended to limit the disclosure.
In the present disclosure, unless otherwise indicated, terms of orientation such as "upper and lower" are used to refer to upper and lower as defined. The terms such as "first" and "second" are used merely to distinguish one element from another element and do not have order or importance. Additionally, the above-used directional terms are merely used to facilitate description of the present disclosure, and are not meant to indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operate in a particular orientation, and are not to be construed as limiting the present disclosure.
In the present disclosure, unless otherwise stated, terms such as "first direction, second direction" used specifically may refer to fig. 1; terms of orientation such as "inner and outer" are used to refer to inner and outer of a particular structural profile, and terms such as "first and second" are used merely to distinguish one element from another element and are not of sequential or importance.
Referring to fig. 1 to 4, the present disclosure provides a dual wavelength ultraviolet detector including a first optical path component 1, a second optical path component 2, a light splitting sheet 3, a flow cell body 4, a sampling photocell 5, and a reference photocell 6; the first optical path component 1 can emit a first light beam having a first wavelength and being parallel, and the second optical path component 2 can emit a second light beam having a second wavelength and being parallel.
The flow cell body 4 is internally provided with a flow channel 40 for solution to flow and extend along a first direction A, the flow cell body 4 is provided with a first observation port 41 and a second observation port 42, the first observation port 41 and the second observation port 42 are oppositely arranged along a second direction B and are respectively used for being oppositely arranged with the flow channel 40, the light splitting sheet 3 is arranged at the first observation port 41, and the sampling photocell 5 is arranged at the second observation port 42.
The light-splitting sheet 3 can reflect part of the first light beam to sequentially pass through the first observation port 41, the solution in the flow channel 40 and the second observation port 42 along the second direction B so as to shoot to the sampling photocell 5; the beam splitter 3 can also allow a further part of the first light beam to be transmitted towards the reference photocell 6.
The light-splitting sheet 3 can transmit part of the second light beam to sequentially pass through the first observation port 41, the solution in the flow channel 40 and the second observation port 42 along the second direction B so as to shoot to the sampling photocell 5; the beam splitter 3 is also capable of allowing another part of the second light beam to be reflected towards the reference photocell 6; wherein the first direction A and the second direction B are perpendicular to each other.
In the above technical solution, by providing the first optical path component 1 and the second optical path component 2, the first optical path component 1 can emit the first light beam with the first wavelength and the parallel, and the second optical path component 2 can emit the second light beam with the second wavelength and the parallel. A portion of the first beam is reflected by the beam splitter 3 through the solution in the flow channel 40 to be directed to the sampling photocell 5 and another portion of the first beam is transmitted by the beam splitter 3 to be directed to the reference photocell 6. A portion of the light of the second beam is transmitted through the beam splitter 3 and then through the solution in the flow channel 40 to be directed to the use photocell 5, and another portion of the light of the second beam is reflected by the beam splitter 3 and then directed to the reference photocell 6. Because of the different wavelengths of the first and second beams, there is a different degree of energy loss after passing through the solution, respectively, and the concentration of two different substances in the solution can be detected by comparing the sample photocell 5 with the reference photocell 6 according to the beer-lambert law (when a parallel monochromatic light passes perpendicularly through a certain uniform non-scattering light absorbing substance, its absorbance is proportional to the concentration of the light absorbing substance). When detecting, the first light path component 1 and the second light path component 2 can emit light beams in a rapid and alternative way, so that the error in the detection time can be shortened, and the detection precision is improved.
Alternatively, the first wavelength of the first light beam emitted from the first light path component 1 may be 260nm, and the ultraviolet ray of 260nm may detect the DNA in the solution; the second wavelength of the second light beam emitted from the second light path component 2 may be 280nm, and the ultraviolet ray of 280nm may detect the protein in the solution. The present disclosure is not limited to the first wavelength of the first light beam and the second wavelength of the second light beam.
Alternatively, the optical axis of the first optical path component 1 and the optical axis of the second optical path component 2 may be perpendicular to each other, so that the optical paths are arranged.
In one embodiment, referring to fig. 1, the first light path assembly 1 includes a first light source emitter 11 and a first collimating lens 12, the first light source emitter 11 being capable of emitting light of a first wavelength, the first collimating lens 12 being disposed on an emitting side of the first light source emitter 11 for collimating the light of the first wavelength into a first light beam of a parallel light beam. And/or the second light path assembly 2 includes a second light source emitter 21 and a second collimating lens 22, the second light source emitter 21 being capable of emitting light of a second wavelength, the second collimating lens 22 being disposed on the light emitting side of the second light source emitter 21 for collimating the light of the second wavelength into a second light beam of the parallel light beam.
In this embodiment, the first light source emitter 11 and the second light source emitter 21 are respectively configured to emit light beams of a first wavelength and light beams of a second wavelength, and the emitted light beams are not parallel light beams, so that the light beams of the first wavelength and the light beams of the second wavelength can be respectively collimated into parallel light beams by the first collimating lens 12 and the second collimating lens 22, thereby ensuring that the first light beams and the second light beams can vertically irradiate the solution in the flow channel 40 under the reflection of the light splitting sheet 3, and improving the detection accuracy.
Optionally, referring to fig. 1, the first light path assembly 1 further includes a first filter 13, and the first filter 13 is disposed between the first light source emitter 11 and the first collimating lens 12. And/or, the second light path assembly 2 further comprises a second optical filter 23, the second optical filter 23 being arranged between the second light source emitter 21 and the second collimator lens 22. By providing the first filter 13 and the second filter 23, it is possible to ensure that no parasitic light of other wavelength bands exists in the first light beam of the first wavelength and the second light beam of the second wavelength.
In other embodiments, referring to fig. 1, the first light path assembly 1 further includes a first diaphragm 14, the first diaphragm 14 being disposed between the first light source emitter 11 and the first collimating lens 12; and/or the second light path assembly 2 further comprises a second diaphragm 24, the second diaphragm 24 being arranged between the second light source emitter 21 and the second collimator lens 22. The first diaphragm 14 may limit the imaging range of the first light source emitter 11, and likewise the second diaphragm 24 may limit the imaging range of the second light source emitter 12 to meet the use requirements of the first and second light beams.
In an alternative embodiment, the first light path component 1 further includes a first mounting member (not shown), and the first light source emitter 11, the first diaphragm 14, the first optical filter 13, and the first collimating lens 12 are sequentially disposed on the first mounting member at intervals along the light path direction of the first light path component 1; and/or, the second light path assembly 2 further includes a second mounting member (not shown), and the second light source emitter 21, the second diaphragm 24, the second optical filter 23, and the second collimating lens 22 are sequentially disposed on the second mounting member at intervals along the light path direction of the second light path assembly 2. By providing the first mounting member and/or the second mounting member, the first optical path component 1 and/or the second optical path component 2 can be arranged and mounted.
Optionally, the dual wavelength ultraviolet detector further comprises a controller, the first light source emitter 11 and the second light source emitter 21 are each electrically connected to the controller, and the controller is operable to: the first light source emitters 11 and the second light source emitters 21 are controlled to alternately emit light periodically, and the degree of automation is high. In addition, the light emission interval time of the first light source emitter 11 and the second light source emitter 21 may be as short as possible to shorten the time error as much as possible.
Alternatively, the beam splitter 3 extends in the first direction a, the first light beam having an angle of incidence and reflection on the beam splitter 3 of 45 ° and the second light beam having an angle of incidence and reflection on the beam splitter 3 of 45 °. So that half of the first beam/second beam is transmitted to the reference photocell 6 and the other half of the first beam/second beam is reflected to the sampling photocell 5 for comparison.
Referring to fig. 2 to 4, the dual wavelength ultraviolet detector further includes a first observation mirror 410 and a second observation mirror 420, the first observation mirror 410 is disposed at the first observation port 41, and the second observation mirror 420 is disposed at the second observation port 42. By providing the first observation mirror 410 and the second observation mirror 420, the first observation port 41 and the second observation port 42 can be plugged, and light can be emitted to the sampling photocell 5.
The preferred embodiments of the present disclosure have been described in detail above with reference to the accompanying drawings, but the present disclosure is not limited to the specific details of the above embodiments, and various simple modifications may be made to the technical solutions of the present disclosure within the scope of the technical concept of the present disclosure, and all the simple modifications belong to the protection scope of the present disclosure.
In addition, the specific features described in the above embodiments may be combined in any suitable manner without contradiction. The various possible combinations are not described further in this disclosure in order to avoid unnecessary repetition.
Moreover, any combination between the various embodiments of the present disclosure is possible as long as it does not depart from the spirit of the present disclosure, which should also be construed as the disclosure of the present disclosure.
Claims (10)
1. The dual-wavelength ultraviolet detector is characterized by comprising a first light path component, a second light path component, a light splitting sheet, a flow cell body, a sampling photocell and a reference photocell; the first light path component can emit a first light beam with a first wavelength and parallel, and the second light path component can emit a second light beam with a second wavelength and parallel;
the flow cell comprises a flow cell body, wherein a flow channel for flowing solution and extending along a first direction is formed in the flow cell body, a first observation port and a second observation port are formed in the flow cell body, the first observation port and the second observation port are oppositely arranged along a second direction and are both used for being oppositely arranged with the flow channel, the light splitting sheet is arranged at the first observation port, and the sampling photocell is arranged at the second observation port;
the light splitting sheet can reflect part of the first light beam to sequentially pass through the first observation port, the solution in the flow channel and the second observation port along the second direction so as to irradiate the sampling photocell; the beam splitter sheet is further capable of allowing another portion of the first light beam to be transmitted toward the reference photocell;
the light splitting sheet can transmit part of the second light beam to sequentially pass through the first observation port, the solution in the flow channel and the second observation port along the second direction so as to irradiate the sampling photocell; the beam splitter sheet is further capable of allowing another portion of the second light beam to reflect toward the reference photocell; wherein the first direction and the second direction are perpendicular to each other.
2. The dual wavelength ultraviolet detector of claim 1, wherein the first light path assembly comprises a first light source emitter capable of emitting light of a first wavelength and a first collimating lens disposed on an exit side of the first light source emitter for collimating the light of the first wavelength into a first light beam of a parallel light beam; and/or the number of the groups of groups,
the second light path component comprises a second light source emitter and a second collimating lens, the second light source emitter can emit light rays with a second wavelength, and the second collimating lens is arranged on the light emitting side of the second light source emitter and is used for collimating the light rays with the second wavelength into a second light beam of parallel light beams.
3. The dual wavelength ultraviolet detector of claim 2, wherein the first light path assembly further comprises a first filter disposed between the first light source emitter and the first collimating lens; and/or the number of the groups of groups,
the second light path component further comprises a second optical filter, and the second optical filter is arranged between the second light source emitter and the second collimating lens.
4. The dual wavelength ultraviolet detector of claim 3, wherein the first light path assembly further comprises a first diaphragm disposed between the first light source emitter and the first collimating lens; and/or the number of the groups of groups,
the second light path assembly further comprises a second diaphragm, and the second diaphragm is arranged between the second light source emitter and the second collimating lens.
5. The dual wavelength ultraviolet detector of claim 4, wherein the first light path assembly further comprises a first mounting member, the first light source emitter, the first diaphragm, the first optical filter, and the first collimating lens being sequentially disposed at intervals in the first mounting member along the light path direction of the first light path assembly; and/or the number of the groups of groups,
the second light path component further comprises a second mounting piece, and the second light source emitter, the second diaphragm, the second optical filter and the second collimating lens are sequentially arranged on the second mounting piece at intervals along the light path direction of the second light path component.
6. The dual wavelength ultraviolet detector of claim 2, further comprising a controller, wherein the first light source emitter and the second light source emitter are each electrically connected to the controller, and wherein the controller is operable to: the first light source emitter and the second light source emitter are controlled to periodically and alternately emit light.
7. The dual wavelength ultraviolet detector of any one of claims 1-5, wherein the beam splitter plate extends along the first direction, wherein the first beam has an incident angle and a reflection angle of 45 ° on the beam splitter plate, and wherein the second beam has an incident angle and a reflection angle of 45 ° on the beam splitter plate.
8. The dual wavelength ultraviolet detector of any one of claims 1-5, further comprising a first viewing mirror disposed at the first viewing port and a second viewing mirror disposed at the second viewing port.
9. The dual wavelength ultraviolet detector of any one of claims 1-5, wherein an optical axis of the first optical path assembly is perpendicular to an optical axis of the second optical path assembly.
10. The dual wavelength ultraviolet detector of any one of claims 1-5, wherein the first wavelength is 260nm and the second wavelength is 280nm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322025221.1U CN220626198U (en) | 2023-07-28 | 2023-07-28 | Dual wavelength ultraviolet detector |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322025221.1U CN220626198U (en) | 2023-07-28 | 2023-07-28 | Dual wavelength ultraviolet detector |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220626198U true CN220626198U (en) | 2024-03-19 |
Family
ID=90214742
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322025221.1U Active CN220626198U (en) | 2023-07-28 | 2023-07-28 | Dual wavelength ultraviolet detector |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220626198U (en) |
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2023
- 2023-07-28 CN CN202322025221.1U patent/CN220626198U/en active Active
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