CN220530056U - Cell collection device - Google Patents
Cell collection device Download PDFInfo
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- CN220530056U CN220530056U CN202321624360.XU CN202321624360U CN220530056U CN 220530056 U CN220530056 U CN 220530056U CN 202321624360 U CN202321624360 U CN 202321624360U CN 220530056 U CN220530056 U CN 220530056U
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- Prior art keywords
- cell
- spring
- structural support
- spring tube
- tube
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- 210000004027 cell Anatomy 0.000 claims abstract description 45
- 239000011248 coating agent Substances 0.000 claims abstract description 24
- 238000000576 coating method Methods 0.000 claims abstract description 24
- 238000003306 harvesting Methods 0.000 claims abstract description 15
- 230000000694 effects Effects 0.000 claims abstract description 8
- 230000003466 anti-cipated effect Effects 0.000 claims abstract description 4
- 210000005260 human cell Anatomy 0.000 claims abstract description 4
- 102000018651 Epithelial Cell Adhesion Molecule Human genes 0.000 claims description 5
- 108010066687 Epithelial Cell Adhesion Molecule Proteins 0.000 claims description 5
- 229910001000 nickel titanium Inorganic materials 0.000 claims description 5
- 239000010935 stainless steel Substances 0.000 claims description 4
- 229910001220 stainless steel Inorganic materials 0.000 claims description 4
- 102000008394 Immunoglobulin Fragments Human genes 0.000 claims description 3
- 108010021625 Immunoglobulin Fragments Proteins 0.000 claims description 3
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 3
- 150000007523 nucleic acids Chemical group 0.000 claims description 3
- 238000007373 indentation Methods 0.000 claims description 2
- 238000003466 welding Methods 0.000 claims description 2
- HLXZNVUGXRDIFK-UHFFFAOYSA-N nickel titanium Chemical compound [Ti].[Ti].[Ti].[Ti].[Ti].[Ti].[Ti].[Ti].[Ti].[Ti].[Ti].[Ni].[Ni].[Ni].[Ni].[Ni].[Ni].[Ni].[Ni].[Ni].[Ni].[Ni].[Ni].[Ni].[Ni] HLXZNVUGXRDIFK-UHFFFAOYSA-N 0.000 claims 1
- 239000008280 blood Substances 0.000 description 15
- 210000004369 blood Anatomy 0.000 description 15
- 206010028980 Neoplasm Diseases 0.000 description 4
- 201000011510 cancer Diseases 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 238000003745 diagnosis Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000002202 Polyethylene glycol Substances 0.000 description 2
- 208000007536 Thrombosis Diseases 0.000 description 2
- WAIPAZQMEIHHTJ-UHFFFAOYSA-N [Cr].[Co] Chemical class [Cr].[Co] WAIPAZQMEIHHTJ-UHFFFAOYSA-N 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 239000000017 hydrogel Substances 0.000 description 2
- 229920001223 polyethylene glycol Polymers 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 208000005443 Circulating Neoplastic Cells Diseases 0.000 description 1
- 229920002125 Sokalan® Polymers 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 238000010241 blood sampling Methods 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 238000009998 heat setting Methods 0.000 description 1
- 230000002439 hemostatic effect Effects 0.000 description 1
- 229920001477 hydrophilic polymer Polymers 0.000 description 1
- 238000010147 laser engraving Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 239000002861 polymer material Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
A cell harvesting device, comprising: a structural support element, the structural support element being a conical helical filiform structure; the spring tube is wrapped on the surface of the structural supporting element and is in a conical spiral shape under the action of the structural supporting element; the collecting coating is coated on the surface of the spring tube and can specifically capture human cells; a rotating handle connected to the structural support element for transitioning the spring tube between a conical helical configuration and a flat configuration. Before the use, twist grip is in the state of screwing up, at this moment the spring pipe is straight structure, is convenient for carry, will after the spring pipe carries the anticipated position, unscrew the twist grip, the spring pipe will resume conical spiral structure under elastic effect, the cell will enrich under the effect of collection coating on the spring pipe.
Description
Technical Field
The utility model relates to the field of medical instruments, in particular to a cell acquisition device.
Background
The collection of relevant cells in human blood for disease diagnosis or treatment by detection or culture of the cells has been increasingly used. At present, most of cell collection is performed by blood sampling, and then required cells are extracted from sampled blood, but the requirements of efficient diagnosis and treatment of diseases cannot be met because the concentration of relevant cells is often low.
CN205054227U describes a cell collection device that can be introduced directly into the circulating blood and then collect specific cells under the influence of an antibody layer, but the device has a smaller collector surface area and cannot collect an effective number of cells. In addition, the device has a long residence time in blood and risks thrombus formation.
Disclosure of Invention
The utility model aims to overcome the defects and provide a cell acquisition device.
The technical scheme adopted by the utility model is as follows:
a cell harvesting device, comprising:
a structural support element, the structural support element being a conical helical filiform structure;
the spring tube is wrapped on the surface of the structural supporting element and is in a conical spiral shape under the action of the structural supporting element;
the collecting coating is coated on the surface of the spring tube and can specifically capture human cells;
a rotating handle connected to the structural support element for transitioning the spring tube between a conical helical configuration and a flat configuration.
Before the use, twist grip is in the state of screwing up, at this moment the spring pipe is straight structure, is convenient for carry, will after the spring pipe carries the anticipated position, unscrew the twist grip, the spring pipe will resume conical spiral structure under elastic effect, the cell will enrich under the effect of collection coating on the spring pipe.
Preferably, the rear end of the structural support member further has a straight section extending around the rotatable handle.
Preferably, the pushing device further comprises a pushing tube, the straight section passes through the pushing tube, and one end of the pushing tube is connected with the rotating handle.
Preferably, the device further comprises a loading sleeve for protecting the spring tube and the collecting coating thereon, wherein the spring tube is contracted in the loading sleeve when not in use, and is pushed out of the loading sleeve into blood by a pushing rod when in use.
Preferably, the collection coating comprises an antibody, antibody fragment, amino acid structure, nucleic acid structure, or synthetic structure having specific affinity for the cell surface.
Preferably, the acquisition coating comprises EpCAM antibodies.
Preferably, the surface of the spring tube is provided with micro-holes, indentations or dimples for increasing the surface area to collect more cells.
Preferably, the spring tube further comprises a wire, wherein the wire is adhered to the structural support element and extends to the outside through the gap of the spring tube to form a tentacle structure.
Preferably, the surface of the wire is coated with a harvesting coating.
Preferably, the structural support element and the spring tube are made of nickel-titanium alloy or stainless steel.
Preferably, the end of the spring tube opposite to the rotary handle is fixed to the structural support element by means of welding.
Drawings
FIG. 1 shows a cell collection device, i.e., the present utility model;
FIG. 2 is a detail view of the spring tube and structural support member;
FIG. 3 a state in which the spring tube is stretched straight;
FIG. 4 is a cell collection device sleeved with a loading cannula;
FIG. 5 is a cell collection device provided with a wire.
Description of the embodiments
Embodiments of the present utility model are described in further detail below with reference to the accompanying drawings and examples. The following examples are illustrative of the utility model but are not intended to limit the scope of the utility model.
A cell harvesting device, comprising:
a structural support element 100, said structural support element being a conical helical filiform structure; a spring tube 200, which is wrapped on the surface of the structural support element, and one end of which is welded with the structural support element, and under the action of the structural support element, the spring tube is also conical and spiral; the collecting coating is coated on the surface of the spring tube, can specifically capture human cells and prevent thrombus; a rotating handle 300 is connected to the structural support element for translating the spring tube between a conical helical configuration and a flat configuration.
Before the use, twist grip is in the state of screwing up, at this moment the spring pipe is straight structure, is convenient for carry, will after the spring pipe carries the anticipated position, unscrew the twist grip, the spring pipe will resume conical spiral structure under elastic effect, the cell will enrich under the effect of collection coating on the spring pipe.
The structure supporting element 100 and the spring tube 200 together form a collection part, and the structure supporting element is in a wire shape and is used for keeping the spiral structure of the collection part, so that the contact area between blood and the collection part can be increased, and the number of cells relevant to collection can be increased. The structural support element 100 is a metal structure and is fixed into a conical spiral structure by a heat setting mode, and the preferred material of the structural support element 100 is nickel-titanium alloy, because the nickel-titanium alloy has super elasticity, the elasticity is far greater than that of common materials, and the structural support element can quickly recover to the conical spiral structure after entering blood. The material of the structural support member 100 may be stainless steel, cobalt-chromium alloy, or the like.
The spring tube 200 is an elongated spring-like structure with good flexibility, the shape of which can be changed along with the structural support element 100, the surface of the spring tube is arc-shaped and convex, and gaps exist between each layer, so that the contact surface between the spring tube and blood can be increased, and the collection amount of relevant cells can be improved. Preferably, the spring tube is made of stainless steel, which has relatively small elasticity, so that its shape can be changed according to the structural support member 100. The spring tube 200 may be made of nickel-titanium alloy, cobalt-chromium alloy, or the like.
The main body of the structural support member 100 has a conical spiral structure, and a flat section is further provided at the end of the main body, and the flat section extends and is wound around the rotating handle 300, and the structural support member 100 can be pulled by rotating the rotating handle 300, so that the spring tube can be straightened, as shown in fig. 3. When the spring tube 200 is in a relaxed state, the spring tube is in a spiral structure under the action of the structural support member 100, and a certain gap exists between each layer of the spring tube and is relatively in a certain angle, as shown in fig. 2, when the spring tube 200 is tightened by rotating the handle 300, the layers are tightly adhered to each other and are in a parallel state, and the gap also disappears, so that the spring tube 200 becomes a flat state when seen from the whole. When the spring tube 200 is released by rotating the handle 300, the spring tube 200 is restored to the spiral structure by the elasticity of the structural support member 100.
The utility model further comprises a pushing tube 400, wherein the straight section at the tail end of the structural support element 100 passes through the pushing tube, and one end of the pushing tube 400 is fixedly connected with the rotary handle 300. On the one hand, the pushing tube 400 provides a fixed platform for the rotary handle 300, and on the other hand, the pushing tube 400 controls the forward and backward movement of the collecting part during conveying.
The present utility model also includes a loading cannula 500 for protecting the spring tube and the acquisition coating thereon, the spring tube being retracted within the loading cannula when not in use, as shown in fig. 4, and the spring tube being pushed out of the loading cannula into the blood by a push rod when in use. The loading sleeve is made of high polymer materials, the inner wall is smooth, and the collecting coating is not damaged during pushing.
The collection coating includes antibodies, antibody fragments, amino acid structures, nucleic acid structures, or synthetic structures having specific affinity for the cell surface. Such as EpCAM antibodies, which can specifically bind to circulating tumor cells, and can be replaced with other antibodies, as desired.
In order to effectively fix the acquisition coating to the surface of the spring tube 200, it is also necessary that some substances are used as an intermediate layer, preferably hydrogels with carbon-containing long chain branched macromolecules, which have a large number of functional groups, for example carboxyl groups and/or polycarboxylic groups, which can ensure that the acquisition coating is permanently bonded by covalent bonds. Hydrogels are three-dimensionally crosslinked hydrophilic polymers, the major components of which are polyacrylic acid (PAA) and polyethylene glycol (PEG), which absorb liquids such as water, but do not dissolve in themselves.
In order to increase the surface area of the collecting part, micropores, nicks or pits can be arranged on the surface of the spring tube, and the shapes can increase the contact area between the collecting coating and blood, so that the adsorption quantity of cells is increased, and the detection efficiency is improved. Because the acquisition part is smaller, the morphology can be processed and manufactured in a laser engraving mode.
The structural support element can be further provided with a silk thread, the silk thread is adhered to the structural support element and extends to the outside through a gap of the spring tube to form a tentacle structure, and the surface of the silk thread is coated with a collecting coating. In blood, the silk thread will spread along with blood flow, increasing its contact surface with blood, and increasing cell collection amount. In some embodiments, different wires may be coated with different antibodies that capture different cells, and the wires may be removed directly after harvesting for different detection.
The complete using process comprises the following steps: the product is mainly used for cancer diagnosis, when in use, a passage is established in a vein by using a puncture kit, then a loading sleeve is inserted into a hemostatic valve of a puncture sheath, a spring tube is pushed into blood by a pushing tube or a rotating handle, then the rotating handle is unscrewed, at the moment, the spring tube can recover to a conical spiral structure under the elastic action of a structural supporting element, the spring tube remains in the blood for about 30 minutes, an antibody on a collection coating, such as EpCAM (anti-EpCAM), can be combined with protein EpCAM on the surface of cancer cells in circulating blood, so that cancer cells in a body can be specifically collected, and the collected cancer cells can be sent into a laboratory for further detection.
The described embodiments are to be considered in all respects only as illustrative and not restrictive. The scope of the disclosure is, therefore, indicated by the appended claims rather than by the foregoing description. All changes which come within the meaning and range of equivalency of the claims are to be embraced within their scope. The utility model is not limited to the embodiments described above, i.e. it is not meant that the utility model has to be carried out in dependence on the embodiments described above. It should be apparent to those skilled in the art that any modification of the present utility model, equivalent substitution of selected raw materials, addition of auxiliary components, selection of specific modes, etc. fall within the scope of the present utility model and the scope of disclosure.
Claims (11)
1. A cell harvesting device, comprising:
a structural support element, the structural support element being a conical helical filiform structure;
the spring tube is wrapped on the surface of the structural supporting element and is in a conical spiral shape under the action of the structural supporting element;
the collecting coating is coated on the surface of the spring tube and can specifically capture human cells;
a rotating handle connected to the structural support element for transitioning the spring tube between a conical helical configuration and a flat configuration;
before the use, twist grip is in the state of screwing up, at this moment the spring pipe is straight structure, is convenient for carry, will after the spring pipe carries the anticipated position, unscrew the twist grip, the spring pipe will resume conical spiral structure under elastic effect, the cell will enrich under the effect of collection coating on the spring pipe.
2. The cell harvester of claim 1 wherein the tail end of the structural support member further comprises a straight section extending around the rotatable handle.
3. The cell harvesting device of claim 2, further comprising a push tube, wherein the straight section passes inside the push tube, and wherein one end of the push tube is connected to the rotating handle.
4. The cell harvester of claim 1, further comprising a loading sleeve for protecting the spring tube and the harvesting coating thereon, the spring tube being retracted within the loading sleeve when not in use.
5. The cell harvester of claim 1, wherein the harvesting coating comprises an antibody, an antibody fragment, an amino acid structure, a nucleic acid structure, or a synthetic structure having specific affinity for a cell surface.
6. The cell harvester of claim 5 wherein the harvesting coating comprises EpCAM antibody.
7. A cell harvesting device according to claim 1, wherein the spring tube surface is provided with micro-holes, indentations or dimples for increasing the surface area for harvesting more cells.
8. The cell harvesting device of claim 1, further comprising a wire bonded to the structural support member and extending through the slit of the spring tube to the outside to form a tentacle structure.
9. The cell harvester of claim 8, wherein the wire surface is coated with a harvesting coating.
10. The cell harvester of claim 1 wherein the structural support element and the spring tube are made of nitinol or stainless steel.
11. The cell harvesting device of claim 1, wherein an end of the spring tube opposite the rotatable handle is secured to the structural support member by welding.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321624360.XU CN220530056U (en) | 2023-06-26 | 2023-06-26 | Cell collection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321624360.XU CN220530056U (en) | 2023-06-26 | 2023-06-26 | Cell collection device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220530056U true CN220530056U (en) | 2024-02-27 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321624360.XU Active CN220530056U (en) | 2023-06-26 | 2023-06-26 | Cell collection device |
Country Status (1)
| Country | Link |
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| CN (1) | CN220530056U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN118924381A (en) * | 2024-10-14 | 2024-11-12 | 上海宏普医疗器械有限公司 | A multi-stage thrombus removal device |
-
2023
- 2023-06-26 CN CN202321624360.XU patent/CN220530056U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN118924381A (en) * | 2024-10-14 | 2024-11-12 | 上海宏普医疗器械有限公司 | A multi-stage thrombus removal device |
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