CN213986125U - Flow cytometry and sheath flow control pipeline thereof - Google Patents
Flow cytometry and sheath flow control pipeline thereof Download PDFInfo
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- CN213986125U CN213986125U CN202023124416.4U CN202023124416U CN213986125U CN 213986125 U CN213986125 U CN 213986125U CN 202023124416 U CN202023124416 U CN 202023124416U CN 213986125 U CN213986125 U CN 213986125U
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Abstract
The utility model provides a flow cytometer and sheath flow control pipeline thereof belongs to medical equipment technical field. The sheath flow control pipeline of the flow cytometry comprises a sheath fluid container and a flow chamber, wherein the sheath fluid container is used for providing sheath fluid, the flow chamber is communicated with the sheath fluid container through a connecting pipe assembly, a pump assembly which is composed of a plurality of sheath flow pumps used for pumping and discharging the sheath fluid to form sheath flow is arranged on the connecting pipe assembly, the pump assembly is electrically connected with a controller, the controller enables split flow pulse signals generated by the sheath flow pumps to be superposed to form a total flow pulse signal in a horizontal straight line shape through controlling the sheath flow pumps in the pump assembly, and therefore the flow of the sheath fluid conveyed to the flow chamber through the connecting pipe assembly is always kept stable. The flow cytometry and the sheath flow control pipeline thereof have the advantages that: the total flow of sheath flow liquid input into the flow chamber is conveniently controlled through the parallel connecting branch pipes and the sheath flow pumps arranged on the parallel connecting branch pipes so as to realize stable operation of the total flow.
Description
Technical Field
The utility model belongs to the technical field of medical equipment, especially, relate to a flow cytometry and sheath flow control pipeline thereof.
Background
The flow cytometry (BD LSRFortessa) mainly comprises a light source, a flow channel, a signal detection transmission and data analysis system, has the functions of qualitatively and quantitatively analyzing various cell components in cell membranes, cytoplasm and cell nucleus, and also can research various functional states of cells, such as cell proliferation, cell apoptosis, cell differentiation, enzyme activity, cell membrane fluidity, membrane potential, membrane permeability, redox state, phagocytosis, intracellular ion concentration and the like. The method is an important component of clinical detection in the current clinical detection of peripheral blood leucocyte, bone marrow cell, tumor cell and the like by using a flow cytometer.
Sheath flow is a technique to avoid the effects of turbulence, and flow of blood cells through the edges of the well during counting, and is directed at the well by a capillary tube from which the cell suspension is ejected. And simultaneously, the cell suspension and sheath liquid flowing out from the periphery (the sheath liquid is a balanced electrolyte solution without fluorescence background, the main components of the sheath liquid are sodium chloride, potassium chloride, ethylene diamine tetraacetic acid disodium and a bacteriostatic agent, and the sheath liquid is used as a flow cytometry analyzer for analyzing the physicochemical and biological characteristics of biological particles such as cells) flow through a sensitive area together, so that the cell suspension is ensured to form a singly arranged cell flow in the middle and the periphery is surrounded by the sheath liquid.
In the current flow cytometric analyzers, a sheath flow is generated by a plunger pump and a pulse absorber, and then a gas cylinder is used to pressurize a square flow chamber. According to the working principle of the plunger pump, the plunger pump can only perform one of liquid suction action and liquid discharge action at the same time and cannot simultaneously operate, so that the problems of discontinuous liquid supply, large flow fluctuation, need of regular maintenance on the pulse absorber, relatively complex structure and the like are caused.
SUMMERY OF THE UTILITY MODEL
It is an object of the present invention to provide a sheath flow control circuit that solves at least some of the above problems.
It is another object of the present invention to provide a flow cytometer having the sheath flow control circuit described above.
In order to achieve the above purpose, the utility model adopts the following technical proposal: the utility model discloses a sheath flow control pipeline of flow cytometry, including sheath liquid container and the flow chamber that is used for providing sheath liquid, through connecting pipe subassembly intercommunication between sheath liquid container and the flow chamber, be provided with on the connecting pipe subassembly by a plurality of taking out that are used for, arrange the sheath liquid with the sheath flow pump subassembly that forms of formation sheath flow, the pump subassembly is connected with the controller electricity, each sheath flow pump in the controller passes through the control pump subassembly so that form one after the reposition of redundant personnel pulse signal stack that each sheath flow pump produced and be the linear total flow pulse signal of level, so that the sheath liquid flow through the connecting pipe subassembly is defeated to flow chamber that flows and is kept steady all the time.
In the sheath flow control pipeline in the flow cytometry, the connecting pipe assembly comprises a plurality of connecting branch pipes which are arranged in parallel, one end of each connecting branch pipe is communicated with the flow chamber, the other end of each connecting branch pipe is communicated with the sheath liquid container, and each connecting branch pipe is provided with a sheath flow pump.
In the sheath flow control pipeline in the flow cytometer, the connecting pipe assembly further includes an output main pipe, one end of the output main pipe is communicated with the flow chamber, and the other end of the output main pipe is communicated with one end of each connecting branch pipe.
In the sheath flow control pipeline in the flow cytometry analyzer, the flow pulse signals output by each sheath flow pump in the control pump assembly during operation are the same, and the controller controls the starting time and the stopping time of each sheath flow pump in the control pump assembly so as to enable the flow of the sheath liquid input into the flow chamber through the connecting pipe assembly to be always kept stable.
In the sheath flow control line in the flow cytometer described above, the sheath flow pump is a gap pump.
In the sheath flow control line in the flow cytometer described above, the sheath flow pump is a plunger pump.
The flow cytometer includes the sheath flow control circuit.
Compared with the prior art, the flow cell analyzer and the sheath flow control pipeline thereof have the advantages that: the total flow of sheath flow liquid input into the flow chamber is conveniently controlled through the parallel connecting branch pipes and the sheath flow pumps arranged on the parallel connecting branch pipes so as to realize stable operation of the total flow.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
Fig. 1 provides a tubing diagram of one embodiment of a sheath flow control tubing in an embodiment of the present invention.
Fig. 2 provides a graph of the respective partial flow pulse signals and the total flow pulse signals generated after superposition when all sheath flow pumps in one embodiment of the sheath flow control circuit of the present invention are operated.
In the figure, a sheath liquid container 101, a flow cell 102, a plunger pump 103, a connecting branch pipe 104, and an output manifold 105.
Detailed Description
The present invention will be described in further detail by way of examples with reference to the accompanying drawings, which are illustrative of the present invention and are not intended to limit the present invention.
Flow cytometers typically consist of a light source, flow channels, signal detection transmission, and an analytical system for data.
The flow channel in the flow cytometer is a sheath flow control channel described below.
As shown in fig. 1 to 2, the sheath flow control circuit of the flow cytometer includes a sheath fluid container 101 and a flow chamber 102 for providing a sheath fluid, the sheath fluid container 101 and the flow chamber 102 are communicated with each other through a connection pipe assembly, the connection pipe assembly is provided with a pump assembly composed of a plurality of sheath flow pumps for pumping and discharging the sheath fluid to form a sheath flow, the pump assembly is electrically connected to a controller, and the controller controls each sheath flow pump in the pump assembly to superpose split flow pulse signals generated by each sheath flow pump to form a horizontal linear total flow pulse signal, so that the flow of the sheath fluid delivered to the flow chamber 102 through the connection pipe assembly is always kept stable.
It should be noted that the controller is usually a micro circuit control chip (MCU), but may also be a PLC programmable control circuit, or another type, specifically selected according to the requirement, and the controller controls the operation of each sheath flow pump by sending corresponding pulse instructions to achieve the result shown in fig. 2.
Specifically, the connecting pipe assembly comprises a plurality of connecting branch pipes 104 arranged in parallel, one end of each connecting branch pipe 104 is communicated with the flow chamber 102, the other end of each connecting branch pipe is communicated with the sheath fluid container 101, and each connecting branch pipe 104 is provided with a sheath flow pump.
It should be noted that, unlike the prior art in which only one pipeline is introduced into the flow chamber 102, the pipeline is provided with the parallel connecting branch pipes 104, so that a plurality of branch flows are simultaneously introduced into the flow chamber 102, which is beneficial to stably controlling the total flow entering the flow chamber 102.
It should be noted that the number of the connecting branch pipes 104 and the number of the sheath flow pumps are not limited and may be determined according to specific needs, and the magnitude of the output flow rate of the sheath flow pumps and the respective divided flow rate pulse signals are not required to be the same, and the controller may control the start-stop time, the frequency, and other parameters of the motors on the designated several sheath flow pumps according to the required flow rate and the maximum output flow rate of each sheath flow pump so as to realize the stable supply of the required sheath flow rate as long as the sheath flow rate that can provide stable flow rate to the flow chamber 102 during operation is satisfied.
In one or some embodiments, the connector tube assembly further includes an output manifold 105, one end of the output manifold 105 being in communication with the flow chamber 102 and the other end being in communication with one end of all of the connector tubes 104.
In one or more embodiments, the controller controls the flow pulse signals output by each of the sheath pumps in the pump assembly to be the same during operation, and controls the start time and the stop time of each of the sheath pumps in the pump assembly to maintain the sheath flow rate through the connecting tube assembly into the flow chamber 102 to be constant.
It should be noted that the flow pulse signals of the sheath flow pump are the same, which facilitates the control of the controller.
In one or some embodiments, the sheath flow pump is a gap pump, as shown in fig. 1, which is a plunger pump 103.
It should be noted that the plunger pump 103 is used here, because the plunger pump 103 has a characteristic of supplying a stable flow, rather than a conventional peristaltic pump, which can continuously supply a sheath flow, but has a characteristic of supplying an unstable flow.
The working principle of one embodiment of the sheath flow control circuit as shown in figures 1 to 2: the two connecting branch pipes 104 are respectively provided with a plunger pump 103 with the same flow pulse signal, the plunger pump 103 on one connecting branch pipe 104 is firstly started by the controller to intermittently provide sheath flow liquid to the flow chamber 102 in a night-sucking and liquid-discharging mode, after the delay of half a pulse period, the plunger pump 103 on the other connecting branch pipe 104 is started to intermittently provide the sheath flow liquid to the flow chamber 102 in the night-sucking and liquid-discharging mode, and when the sheath flow liquid supply is stopped, the plunger pump 103 on the two connecting branch pipes 104 is controlled by the controller to stop running.
The specific embodiments described herein are merely illustrative of the spirit of the invention. Various modifications, additions and substitutions for the specific embodiments described herein may be made by those skilled in the art without departing from the spirit of the invention or exceeding the scope of the invention as defined in the accompanying claims.
Although the terms sheath fluid reservoir 101, flow chamber 102, plunger pump 103, connecting manifold 104, output manifold 105, etc. are used more generally herein, the possibility of using other terms is not excluded. These terms are used merely to more conveniently describe and explain the nature of the present invention; they are to be construed in a manner that is inconsistent with the spirit of the invention.
Claims (7)
1. A sheath flow control circuit in a flow cytometer, comprising a sheath fluid reservoir (101) for providing a sheath fluid and a flow cell (102), characterized by: the sheath fluid container (101) is communicated with the flow chamber (102) through a connecting pipe assembly, a pump assembly consisting of a plurality of sheath flow pumps for pumping and discharging sheath fluid to form sheath flow is arranged on the connecting pipe assembly, the pump assembly is electrically connected with the controller, and the controller controls each sheath flow pump in the pump assembly to enable split flow pulse signals generated by each sheath flow pump to be superposed to form a horizontal linear total flow pulse signal, so that the flow of the sheath fluid conveyed to the flow chamber (102) through the connecting pipe assembly is always kept stable.
2. The sheath flow control circuit of claim 1, wherein the connecting tube assembly comprises a plurality of connecting branched tubes (104) arranged in parallel, one end of each of the connecting branched tubes (104) is communicated with the flow chamber (102), the other end of each of the connecting branched tubes is communicated with the sheath fluid container (101), and each of the connecting branched tubes (104) is provided with a sheath flow pump.
3. The sheath flow control circuit of claim 2, wherein the connecting tube assembly further comprises an output manifold (105), one end of the output manifold (105) is connected to the flow chamber (102), and the other end is connected to one end of all the connecting branch tubes (104).
4. The sheath flow control circuit of claim 1, wherein the flow pulse signals output by each sheath flow pump of the control pump assembly during operation are the same, and the controller controls the start time and the stop time of each sheath flow pump of the pump assembly so that the sheath flow rate is always kept stable when the sheath flow rate is input into the flow chamber (102) through the connecting pipe assembly.
5. The sheath flow control circuit of claim 1, wherein the sheath flow pump is a gap pump.
6. The sheath flow control circuit of claim 5, wherein the sheath flow pump is a plunger pump (103).
7. A flow cytometer, comprising: the analyzer includes a sheath flow control circuit as claimed in any one of claims 1 to 6.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202023124416.4U CN213986125U (en) | 2020-12-23 | 2020-12-23 | Flow cytometry and sheath flow control pipeline thereof |
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| CN202023124416.4U CN213986125U (en) | 2020-12-23 | 2020-12-23 | Flow cytometry and sheath flow control pipeline thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115078324A (en) * | 2022-06-30 | 2022-09-20 | 嘉兴市唯真生物科技有限公司 | High-flux flow type fluorescence detection method, intelligent terminal and storage medium |
| CN116359484A (en) * | 2023-05-31 | 2023-06-30 | 深圳市帝迈生物技术有限公司 | Sample analyzer |
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2020
- 2020-12-23 CN CN202023124416.4U patent/CN213986125U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115078324A (en) * | 2022-06-30 | 2022-09-20 | 嘉兴市唯真生物科技有限公司 | High-flux flow type fluorescence detection method, intelligent terminal and storage medium |
| CN115078324B (en) * | 2022-06-30 | 2023-03-10 | 嘉兴市唯真生物科技有限公司 | High-throughput flow-type fluorescence detection method, intelligent terminal and storage medium |
| CN116359484A (en) * | 2023-05-31 | 2023-06-30 | 深圳市帝迈生物技术有限公司 | Sample analyzer |
| CN116359484B (en) * | 2023-05-31 | 2023-09-15 | 深圳市帝迈生物技术有限公司 | Sample analyzer |
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