CN212933126U - Microscope compatible optical sheet imaging system - Google Patents
Microscope compatible optical sheet imaging system Download PDFInfo
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- CN212933126U CN212933126U CN202020555948.4U CN202020555948U CN212933126U CN 212933126 U CN212933126 U CN 212933126U CN 202020555948 U CN202020555948 U CN 202020555948U CN 212933126 U CN212933126 U CN 212933126U
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- 238000003384 imaging method Methods 0.000 title claims abstract description 46
- 230000003287 optical effect Effects 0.000 title claims description 56
- 238000005286 illumination Methods 0.000 claims abstract description 22
- 239000013307 optical fiber Substances 0.000 claims description 22
- 238000001914 filtration Methods 0.000 claims description 3
- 230000015572 biosynthetic process Effects 0.000 abstract description 13
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Abstract
The utility model discloses a microscope compatible formula slide imaging system, slide illuminator in the system installs in the both sides of microscopical microscope objective, uses single microscope objective can realize slide formation of image and traditional microscopic formation of image simultaneously, and under the cooperation of electronic objective table, this system can also realize the microscopic formation of image of three-dimensional slide, and in addition, slide illuminator utilizes optic fibre conduction illumination laser, has reduced slide illuminator's overall dimension, has increased the compactedness of system.
Description
Technical Field
The utility model belongs to the technical field of optics, concretely relates to microscope compatible formula slide imaging system.
Background
In recent years, light sheet microscopes have attracted much attention. By using a cylindrical lens or a scanning mode, a light sheet can be formed at the imaging focal plane, so that slice scanning is realized at the illumination end, which is beneficial to realizing three-dimensional imaging and improving the signal to noise ratio of an image. However, the special requirements for the placement of the sample by the light sheet microscope make light sheet imaging not easy to implement in conventional microscopes. In the present invention, we will provide a new light sheet imaging system compatible with the conventional microscope.
Disclosure of Invention
The utility model aims at the not enough of prior art, provide a microscope compatible formula slide imaging system, can realize the slide formation of image on traditional microscope.
The utility model provides a technical scheme that its technical problem adopted as follows:
the utility model discloses a slide illuminator and microscope, this system use slide illuminator along microscopical focal plane direction parallel illumination sample on the basis of traditional microscopic formation of image, and microscope self's light source is along vertical microscope's focal plane direction illumination sample, through the switching of light filter in the light filter carousel, realizes slide formation of image and traditional microscopic formation of image simultaneously.
The light sheet illuminator comprises two light sheet illuminating modules and two lasers, wherein one light sheet illuminating module comprises an optical fiber, an optical fiber jumper terminal, a collimating lens and a cylindrical lens, light rays emitted by the lasers are transmitted by the optical fiber and are emitted from the optical fiber jumper terminal, the light rays are collimated and emitted after passing through the collimating lens and are focused to a sample through the cylindrical lens, the two light sheet illuminating modules are arranged on two sides of a microscope objective, and the focuses of the two cylindrical lenses are overlapped with the focus of the microscope objective.
In the light sheet illumination module, laser transmits illumination light through an optical fiber, an exit port of the optical fiber jumper terminal is coincided with a rear focal plane of the collimating lens, the cylindrical lens and the collimating lens are installed in a close fit mode, and a light sheet formed at the focal point of the cylindrical lens is coincided with an imaging focal plane of the microscope objective.
The microscope comprises a microscope base, a microscope light source, an electric objective table, a sample holder, a microscope objective, a light filter turntable, a lens cone and a digital camera, wherein the microscope base is arranged on a laboratory platform, the microscope light source is arranged on the microscope base, the electric objective table is arranged above the microscope light source, the sample holder is arranged in the electric objective table and is used for mounting a sample, the microscope objective is arranged above the sample holder, the focal plane of the microscope objective is superposed with the sample in the sample holder, the microscope objective is arranged below the light filter turntable, the lens cone is arranged above the light filter turntable, a tube lens is arranged in the lens cone, the light of the sample is collimated and emitted after passing through the microscope objective and is focused on the image surface of the digital camera, the central axis of the light piece illuminator is superposed with the central axis of the microscope objective, and two light piece illuminating modules are positioned at two sides, the main optical axis of the light sheet illumination module is superposed with the focal plane of the microscope objective.
The electric objective table is a stepping objective table capable of linearly moving along three directions of XYZ, the movement of the electric objective table in the XY direction can move the sample to the imaging focal plane of the microscope objective, and in the image acquisition process of the digital camera, the movement of the electric objective table in the Z direction can realize the slicing imaging of the sample, so as to acquire the three-dimensional microscopic image of the sample.
The microscope light source is used for illuminating a sample from the direction vertical to the focal plane of the microscope objective, light rays at the sample are collimated and emitted out after passing through the microscope objective and are focused to the image plane of the digital camera after passing through the tube lens in the lens barrel, and traditional microscopic imaging is achieved.
The lens cone is used for connecting the optical filter rotating disc and the digital camera, a tube lens is arranged in the lens cone, the focal plane of the tube lens coincides with the image plane of the digital camera, and the lens cone is used for focusing imaging light rays passing through the microscope objective on the image plane of the digital camera.
The optical filter turntable is internally provided with a fluorescent optical filter and a bright field optical filter, the fluorescent optical filter is used for filtering illumination light with the wavelength of a laser, optical sheet micro-imaging is realized through a fluorescent signal, the bright field optical filter is used for adjusting the light intensity of a microscope light source to realize traditional micro-imaging, and optical sheet imaging and traditional micro-imaging can be simultaneously realized through switching of the fluorescent optical filter and the bright field optical filter in the optical filter turntable.
The utility model has the advantages that:
through the utility model discloses a structure setting can realize a microscope compatible formula slide imaging system, and slide illuminator in the system is installed in the both sides of microscopical microscope objective, uses single microscope objective can realize slide formation of image and traditional microscopic formation of image simultaneously, and under the cooperation of electronic objective table, this system can also realize the microscopic formation of image of three-dimensional slide, and in addition, slide illuminator utilizes optic fibre conduction illumination laser, has reduced slide illuminator's overall dimension, has increased the compactedness of system.
Drawings
FIG. 1 is a schematic diagram of a light sheet illuminator.
FIG. 2 is a schematic diagram of a microscope compatible light sheet imaging system.
Detailed Description
In order to make the public more clearly understand the technical essence and the beneficial effects of the present invention, the applicant shall make the following detailed description by way of examples, but the description of the examples is not a limitation to the solution of the present invention, and any equivalent changes made according to the inventive concept, which are only formal and immaterial, shall be regarded as the technical scope of the present invention.
Example 1
The present invention will be further explained with reference to fig. 1, fig. 2 and example 1.
The utility model discloses a slide illuminator and microscope, this system use slide illuminator along microscopical focal plane direction parallel illumination sample on the basis of traditional microscopic formation of image, and microscope self's light source is along vertical microscope's focal plane direction illumination sample, through the switching of light filter in the light filter carousel, realizes slide formation of image and traditional microscopic formation of image simultaneously.
As shown in the attached figure 1, the optical sheet illuminator comprises two optical sheet illuminating modules, a laser 1-1 and a laser 1-2, wherein the left optical sheet illuminating module comprises an optical fiber 2-1, an optical fiber jumper terminal 3-1, a collimating lens 4-1 and a cylindrical lens 5-1, light emitted by the laser 1-1 is transmitted by the optical fiber 2-1 and is emitted from the optical fiber jumper terminal 3-1, the light is collimated and emitted after passing through the collimating lens 4-1 and is focused to a sample through the cylindrical lens 5-1, the right optical sheet illuminating module comprises an optical fiber 2-2, an optical fiber jumper terminal 3-2, a collimating lens 4-2 and a cylindrical lens 5-2, light emitted by the laser 1-2 is transmitted by the optical fiber 2-2 and is emitted from the optical fiber jumper terminal 3-2, the two light sheet illumination modules are arranged at two sides of a microscope objective lens of the microscope, and the focal points of the two cylindrical lens are superposed with the focal point of the microscope objective lens.
As shown in the attached figure 1, in the light sheet illumination module, laser 1-1 transmits illumination light through an optical fiber 2-1, an exit port of an optical fiber jumper terminal 3-1 is coincided with a rear focal plane of a collimating lens 4-1, a cylindrical lens 5-1 is installed in close contact with the collimating lens 4-1, and a light sheet formed at the focal point of the cylindrical lens 5-1 is coincided with an imaging focal plane of a microscope objective.
As shown in the attached figure 2, the microscope comprises a microscope base 1, a microscope light source 2, an electric stage 3, a sample holder 4, a microscope objective 5, a light filter turntable 6, a lens barrel 7 and a digital camera 8, wherein the microscope base 1 is installed on a laboratory platform, the microscope light source 2 is placed on the microscope base 1, the electric stage 3 is installed above the microscope light source 2, the sample holder 4 is installed in the electric stage 3, the sample holder 4 is used for installing a sample, the microscope objective 5 is installed above the sample holder, a focal plane of the microscope objective 5 coincides with a sample in the sample holder 4, the microscope objective 5 is installed below the light filter turntable 6, the lens barrel 7 is arranged above the light filter turntable 6, a tube lens is installed in the lens barrel 7, light of the sample is collimated and emitted through the microscope objective 5 and is focused on an image plane of the digital camera 8 after passing through the tube lens, a central axis of the illuminator 9 coincides with the central axis of the microscope, the two light sheet illumination modules are positioned at two sides of the microscope objective 5, and the main optical axis of the light sheet illumination modules is superposed with the focal plane of the microscope objective 5.
The electric objective table is a stepping objective table capable of linearly moving along three directions of XYZ, the movement of the electric objective table in the XY direction can move the sample to the imaging focal plane of the microscope objective, and in the image acquisition process of the digital camera, the movement of the electric objective table in the Z direction can realize the slicing imaging of the sample, so as to acquire the three-dimensional microscopic image of the sample.
The microscope light source is used for illuminating a sample from the direction vertical to the focal plane of the microscope objective, light rays at the sample are collimated and emitted out after passing through the microscope objective and are focused to the image plane of the digital camera after passing through the tube lens in the lens barrel, and traditional microscopic imaging is achieved.
The lens cone is used for connecting the optical filter rotating disc and the digital camera, a tube lens is arranged in the lens cone, the focal plane of the tube lens coincides with the image plane of the digital camera, and the lens cone is used for focusing imaging light rays passing through the microscope objective on the image plane of the digital camera.
The optical filter turntable is internally provided with a fluorescent optical filter and a bright field optical filter, the fluorescent optical filter is used for filtering illumination light with the wavelength of a laser, optical sheet micro-imaging is realized through a fluorescent signal, the bright field optical filter is used for adjusting the light intensity of a microscope light source to realize traditional micro-imaging, and optical sheet imaging and traditional micro-imaging can be simultaneously realized through switching of the fluorescent optical filter and the bright field optical filter in the optical filter turntable.
Claims (8)
1. A microscope compatible type optical sheet imaging system is characterized by comprising an optical sheet illuminator and a microscope, wherein the optical sheet illuminator comprises two optical sheet illuminating modules and two lasers, and the microscope comprises a microscope base, a microscope light source, an electric objective table, a sample holder, a microscope objective, an optical filter turntable, a lens cone and a digital camera.
2. The microscope compatible optical sheet imaging system according to claim 1, wherein in the optical sheet illuminator, one optical sheet illuminating module comprises an optical fiber, an optical fiber jumper terminal, a collimating lens and a cylindrical lens, light emitted by the laser is transmitted by the optical fiber and emitted from the optical fiber jumper terminal, collimated and emitted through the collimating lens, and focused to a sample through the cylindrical lens, the two optical sheet illuminating modules are installed on two sides of a microscope objective lens of the microscope, and the focal points of the two cylindrical lenses coincide with the focal point of the microscope objective lens.
3. The microscope compatible optical sheet imaging system according to claim 1, wherein the optical sheet illumination module transmits illumination light through the optical fiber, the exit port of the optical fiber jumper terminal coincides with the back focal plane of the collimating lens, the cylindrical lens is mounted in close contact with the collimating lens, and the optical sheet formed at the focal point of the cylindrical lens coincides with the imaging focal plane of the microscope objective.
4. A microscope compatible light sheet imaging system according to claim 1 wherein in the microscope, the microscope base is arranged on a laboratory platform, a microscope light source is arranged on the microscope base, an electric objective table is arranged above the microscope light source, a sample seat is arranged in the electric objective table, the sample seat is used for installing a sample, a microscope objective is arranged above the sample seat, the focal plane of the microscope objective is superposed with the sample in the sample seat, the microscope objective is arranged below the optical filter turntable, a lens cone is arranged above the optical filter turntable, a tube lens is arranged in the lens cone, the light of the sample is collimated and emitted after passing through the microscope objective, after passing through the tube lens, the lens is focused on the image surface of the digital camera, the axis of the optical sheet illuminator is superposed with the central axis of the microscope objective, the two optical sheet illumination modules are positioned at the two sides of the microscope objective, and the main optical axis of the optical sheet illumination module is superposed with the focal plane of the microscope objective.
5. A microscope compatible light sheet imaging system as claimed in claim 1 wherein the motorized stage is a stepping stage linearly movable along three directions of XYZ, the movement of the motorized stage in the XY direction moves the sample to the imaging focal plane of the microscope objective, and the movement of the motorized stage in the Z direction during image acquisition by the digital camera performs slice imaging of the sample to obtain a three-dimensional microscopic image of the sample.
6. The microscope compatible optical sheet imaging system of claim 1, wherein the microscope light source is used for illuminating the sample from a direction perpendicular to the focal plane of the microscope objective, and the light at the sample passes through the microscope objective and then is collimated and emitted out, and passes through the tube lens in the lens barrel and then is focused on the image plane of the digital camera, thereby realizing the traditional microscopic imaging.
7. The microscope compatible optical sheet imaging system according to claim 1, wherein the lens barrel is used for connecting the optical filter rotating disc and the digital camera, and a tube lens is installed in the lens barrel, and a focal plane of the tube lens coincides with an image plane of the digital camera, and is used for focusing the imaging light passing through the microscope objective lens on the image plane of the digital camera.
8. The microscope compatible optical sheet imaging system according to claim 1, wherein the optical filter turntable is internally installed with a fluorescent filter and a bright field filter, the fluorescent filter is used for filtering illumination light with laser wavelength, optical sheet micro-imaging is realized through fluorescent signals, the bright field filter is used for adjusting light intensity of a microscope light source, traditional micro-imaging is realized, and optical sheet imaging and traditional micro-imaging can be simultaneously realized through switching the fluorescent filter and the bright field filter in the optical filter turntable.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020555948.4U CN212933126U (en) | 2020-04-15 | 2020-04-15 | Microscope compatible optical sheet imaging system |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202020555948.4U CN212933126U (en) | 2020-04-15 | 2020-04-15 | Microscope compatible optical sheet imaging system |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116184648A (en) * | 2023-01-30 | 2023-05-30 | 中国人民解放军总医院 | A glass slide scanning device |
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- 2020-04-15 CN CN202020555948.4U patent/CN212933126U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116184648A (en) * | 2023-01-30 | 2023-05-30 | 中国人民解放军总医院 | A glass slide scanning device |
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