CN211749542U - Endoscopic Raman spectrum detection device for intracavity tissue - Google Patents

Abstract

The utility model provides an intracavity tissue endoscopic Raman spectrum detection device, which comprises a hand-held probe, the hand-held probe has a probe holder and an optical fiber probe; the probe holder comprises a hand-held part and a hollow probe catheter , the hand-held part is connected to one end of the probe conduit; both ends of the probe conduit are open structures, one end of the probe conduit corresponding to the hand-held part is the inlet end, and the other end is the detection end; the A fiber optic probe is passed through the inlet end and fixed in the probe conduit. The utility model is suitable for a nasopharyngeal endoscope, does not occupy the biopsy channel of the nasopharyngeal endoscope, reserves a biopsy channel and a channel for extracting mucus in the cavity for doctors, and the hand-held probe can not only be used with an empty endoscope with a biopsy hole It can also be used in conjunction with non-biopsy endoscopes or rigid nasopharyngeal endoscopes to detect nasopharyngeal tissue. The utility model can also be applied to oral mirrors, hysteroscopes, etc., so as to meet the observation needs of different parts in the human cavity.

Description

A device for endoscopic Raman spectroscopy detection of intracavity tissue

【Technical field】

The utility model particularly relates to an endoscopic Raman spectrum detection device for intracavity tissue.

【Background technique】

Nasopharyngeal carcinoma is one of the malignant tumors that occur in the head and neck, mainly in China's Fujian Province, Guangdong Province, Hong Kong, Southeast Asia and some countries in Asia. Because nasopharyngeal cancer has no obvious symptoms in the early stage, and the nasopharynx is located deep in the head and neck, early nasopharyngeal cancer is difficult to detect. By the time nasopharyngeal cancer is diagnosed, more than 70% of nasopharyngeal cancer patients are already in the middle and late stages. The survival rate of early stage nasopharyngeal carcinoma is more than 90%, and the survival rate of patients with advanced nasopharyngeal carcinoma is less than 30.3%. Therefore, early diagnosis of nasopharyngeal carcinoma becomes the key to the treatment of nasopharyngeal carcinoma. Conventional diagnostic methods include: nasopharyngoscopy, magnetic resonance imaging (MRI), computed tomography (CT), tissue biopsy, etc., but these methods are expensive, time-consuming, have secondary damage and need to rely on Doctors' clinical experience and other shortcomings, and it is difficult to achieve early diagnosis of nasopharyngeal cancer.

Raman spectroscopy is a non-destructive optical detection technology. It obtains Raman spectra of tissues through the interaction between light and tissues. Raman spectroscopy can provide very important biochemical information and has diagnostic significance for human tissue diseases. Therefore, the research of human tissue Raman spectroscopy is of great significance for early non-destructive cancer screening.

However, the traditional nasopharyngeal endoscope Raman spectroscopy detection device uses a fiber endoscope, which has a large biopsy hole and is relatively easy to install an optical probe. Raman spectroscopy can be detected based on the biopsy channel, but the fiber endoscope is easily damaged. , and using Raman spectroscopy detection, the fiber optic probe must enter the human cavity through the endoscope biopsy channel, because it occupies the biopsy channel used for clamping the pathological test specimen, and at the same time, the intracavitary mucus cannot be removed through the biopsy hole, resulting in Under white light, the field of view is blurred, and abnormal tissue cannot be found. Even if abnormal tissue is found, mucus will interfere with the detection of Raman signals, reducing the signal-to-noise ratio. In addition, the optical fiber probe based on the biopsy channel of the endoscope, the steering angle depends on the endoscope. , Due to the limited bending angle of the endoscope, it cannot be flexibly operated, which will cause a blind spot, and it is easy to touch the mucosa in the cavity, and even cause the mucosa to be damaged and hemorrhage. The measured signal is a blood signal. At present, the nasopharyngeal fiberscope has been replaced by the nasopharyngeal electronic endoscope with high observation clarity, and the biopsy hole of the nasopharyngeal electronic endoscope is smaller or has no biopsy channel. For nasopharyngeal electronic microscopes without biopsy holes, there is no biopsy channel, and the existing Raman probes that pass through the biopsy holes cannot be used. Therefore, it is urgent to develop a device for spectral detection of nasopharyngeal tissue in close combination with electronic nasopharyngoscopes.

【Content of utility model】

In order to overcome the defects of the existing equipment, the utility model provides an endoscopic Raman spectrum detection device for intracavity tissue.

The utility model is realized as follows: an endoscopic Raman spectrum detection device for intracavity tissue, comprising a hand-held probe, the hand-held probe has a probe holder and an optical fiber probe; the probe holder comprises a hand-held part and a hollow The probe catheter, the handle part is connected to one end of the probe catheter; both ends of the probe catheter are open structures, one end of the probe catheter corresponding to the handle part is the inlet end, and the other end is the detection end ; The fiber probe passes through the inlet end and is fixed in the probe conduit.

Further, the probe conduit is provided with a fastener corresponding to the inlet end, the fiber probe is slidably connected in the probe conduit, and the optical fiber probe is fixed to the probe conduit through the fastener Inside.

Further, a quartz glass column is provided at the detection end of the probe conduit, and the optical fiber probe is immediately connected to the quartz glass column.

Further, the front end of the quartz glass column is provided with rounded corners.

Further, the hand-held portion includes a handle and a finger hole, and the finger hole is provided at the lower end of the handle.

Further, the fiber probe includes a Raman excitation fiber, several Raman collection fibers, a fluorescence/reflection excitation fiber, and a fluorescence/reflection collection fiber; the Raman collection fibers form a circle; the The Raman excitation fiber, fluorescence/reflection excitation fiber, and fluorescence/reflection collection fiber are arranged in an equilateral triangle and are all located within the circle.

Further, the front end of the Raman excitation fiber is coated with a low-pass film that allows one wavelength of laser light to pass through, and the front end of the Raman collection fiber is provided with a front end that can cut off the excitation light and allow Raman scattered light with a larger wavelength to pass through. high pass filter.

Further, it also includes a Raman spectrometer, a Raman excitation light source, a white light source, a fluorescence excitation light source, a reflection/fluorescence spectrometer and a detector; the Raman spectrometer is connected to the output ends of several Raman collecting fibers; the Raman The output end of the excitation light source is connected with the Raman excitation fiber; the white light source and the fluorescence excitation light source are connected with the input end of the fluorescence/reflection excitation fiber through the first filter; the reflection/fluorescence spectrometer is connected with the filter wheel through the filter wheel. The output ends of the fluorescence/reflection collecting fibers are connected; the Raman spectrometer and the reflection/fluorescence spectrometer are all connected with the detector.

The utility model has the advantages that: it does not depend on the endoscope, does not occupy the biopsy channel, reserves the biopsy channel and the channel for extracting the mucus in the cavity for the doctor, and is suitable for combining with the nasopharyngeal electron microscope without the biopsy hole; the hand-held probe not only It can be used in conjunction with an empty endoscope with a biopsy hole, and can also detect nasopharyngeal tissue together with an endoscope without a biopsy hole or a nasopharyngeal rigid endoscope. Accurate measurement; improve the signal-to-noise ratio and avoid probe pollution, prolong the service life of the probe; can realize Raman spectrum detection, improve the sensitivity and specificity of detection. The utility model can also be applied to endoscopes with different types of functions, such as nasopharyngoscope, oral mirror, hysteroscope, etc., so as to meet the observation needs of different parts in the human cavity.

【Description of drawings】

The present utility model will be further described below with reference to the accompanying drawings and embodiments.

FIG. 1 is a schematic structural diagram of an intracavity tissue endoscopic Raman spectrum detection device of the present invention.

FIG. 2 is a schematic structural diagram of a probe holder in the present invention.

FIG. 3 is a schematic structural diagram of an optical fiber probe in the utility model.

FIG. 4 is a schematic structural diagram of a preferred embodiment of the hand-held probe in the present invention.

FIG. 5 is a state diagram of a use state of an intracavity tissue endoscopic Raman spectrum detection device of the present invention.

【Detailed ways】

Please refer to FIGS. 1 to 5 , an intracavity tissue endoscopic Raman spectroscopy detection device 100 includes a hand-held probe 1, a Raman spectrometer 2, a Raman excitation light source 3, a white light source 4, a fluorescence excitation light source 5, a reflection/ Fluorescence spectrometer 6 , detector (not shown), endoscope system 7 ; the hand-held probe 1 has a probe holder 11 and a fiber probe 12 .

Please refer to FIG. 1 and FIG. 2 again, the probe holder 11 includes a handle portion 111 and a hollow probe catheter 112, the handle portion 111 is connected to one end of the probe catheter 112; the handle portion 111 includes a handle 111a and a finger hole 111b, the finger hole 111b is provided at the lower end of the handle 111a. The probe conduit 112 has an opening structure at both ends, one end of the probe conduit 112 corresponding to the handle portion 111 is an inlet end 1121, and the other end is a detection end 1122; the fiber probe 12 passes through the inlet end 1121, and is fixed in the probe tube 112, and extends to the probe end 1122. The probe conduit 112 is provided with a fastener 8 corresponding to the inlet end 1121 , the fiber probe 12 is slidably connected in the probe conduit 112 , and the optical fiber probe 12 is fixed to the probe pipe 112 through the fastener 8 . inside the probe catheter 112 . The fiber optic probe 12 can be steered by the fastener 8, and the length of the fiber optic probe 12 entering the probe holder 11 can also be adjusted, so as to ensure that the end face of the detection end 1122 of the fiber optic probe 12 does not contact the human body cavity when the fiber optic probe 12 enters the human body cavity for detection. The mucosal tissue inside the cavity avoids medical risks such as bleeding and infection caused by damage to the mucosal tissue in the cavity; the fastener 8 can be a locking nut, and the optical fiber probe 12 and the probe holder 11 are fixed by the fastener 8. , which can prevent the deviation of the measurement point caused by the tilting and moving of the optical fiber probe 12 during the measurement process, and improve the positioning accuracy of the optical fiber probe 12 to the lesion.

Please refer to FIG. 1 , FIG. 2 and FIG. 3 again. In a preferred embodiment, a quartz glass column 9 is provided at the detection end 1122 of the probe catheter 112 , and the fiber probe 12 abuts on the quartz glass column 9 . The front end of the quartz glass column is provided with rounded corners; in this way, when the detection end 1122 detects the human body cavity, the quartz glass column is inserted into the tissue in the cavity. The front end surface of the quartz glass column 9 is coated with an antireflection film. The quartz glass column 9 not only serves to limit the distance between the optical fiber probe 12 and the tissue, but also obtains a stable optical intracavity tissue signal. The probe bracket 11 and the quartz glass column 9 can avoid the optical fiber probe 12. The direct contact with the tissue protects the cleanliness of the front end of the optical fiber probe 12 and ensures the efficiency of signal collection. At the same time, when the next patient is detected, the probe holder 11 can be directly replaced without cleaning the optical fiber probe 12 again, thereby prolonging the life of the optical fiber probe 12. service life and shortened detection time. The probe holder 11 is made of stainless steel or other materials with rigidity and biocompatibility, and can directly contact the tissue in the human cavity.

Please refer to FIG. 1 and FIG. 3 again, the fiber probe 12 includes a Raman excitation fiber 121, several Raman collection fibers 122, a fluorescence/reflection excitation fiber 123 and a fluorescence/reflection collection fiber 124; The Raman collection fiber 122 is surrounded by a circle O; the Raman excitation fiber 121 , the fluorescence/reflection excitation fiber 123 and the fluorescence/reflection collection fiber 124 are arranged in an equilateral triangle and are all located in the circle O. The Raman excitation fiber 121 , the Raman collection fiber 122 , the fluorescence/reflection excitation fiber 123 and the middle of the fluorescence/reflection collection fiber 124 are assembled into a bundled fiber, which is fixed at the detection end 1122 with a metal sleeve. The three fibers in the middle are equilateral triangular structures, which can make the collected multispectral spectra converge on the same detection point. The Raman spectrometer 2 is connected to the output ends of several Raman collecting fibers 122; the output end of the Raman excitation light source 3 is connected to the Raman excitation fiber 121; The filter 101 is connected to the input end of the fluorescence/reflection excitation fiber 123; the reflection/fluorescence spectrometer 6 is connected to the output end of the fluorescence/reflection collection fiber 124 through the filter wheel 102; the Raman spectrometer 2, Both reflectance/fluorescence spectrometers 6 are connected to the detectors. The detector is connected to a display 103 . The optical fiber probe 12 can obtain the reflection spectrum, fluorescence spectrum and Raman spectrum signals of the tissue in the cavity, in which the Raman signal excitation and reception part, the end face of the Raman excitation fiber 121 is first coated with a low-pass film that allows the excitation light of one wavelength to pass through, and pulls The front end of the Mann collection fiber 122 adopts a high-pass filter that can cut off the excitation light and allow the Raman scattered light with a larger wavelength to pass through, and then is assembled together. Low wavenumber (200～2000cm ^-1 , fingerprint) and high wavenumber (2600～3500cm ^-1 , high wavenumber) of reflection spectrum, fluorescence spectrum and Raman spectrum can be collected, so as to achieve all-round spectral detection of intracavity tissue, Improve detection efficiency. The present invention uses the white light source 4 as the reflection spectrum detection of the excitation light source and the blue light source as the fluorescence spectrum detection of the excitation light.

1 and 5 again, the endoscope system 7 includes an endoscope 71, a second filter 72, a xenon light source 73 and a data processor 74. The endoscope 71, the second filter 72 and The xenon light sources 73 are connected in sequence; the endoscope 71 , the data processor 74 and the display 103 are connected in sequence. The xenon light source 73 outputs white light as the illumination light of the endoscope 71 to illuminate the tissue in the cavity, the data processor 74 can record the white light optical image observed under the endoscope 71 in real time, and the optical fiber probe 12 simultaneously enters the cavity to collect the spectrum The signal is processed in real time and displayed through the display device. The fiber optic probe 12 and the endoscope 71 can enter the human body cavity at the same time to reach the inspected tissue to obtain the white light image of the intracavity tissue and spectral signals such as reflection spectrum, fluorescence spectrum, Raman spectrum, etc., and realize the simultaneous detection of various optical signals. . When the endoscope 71 is a nasopharyngoscope, after disinfection, the nasopharyngoscope is slowly inserted along one nasal cavity of the human body, and the probe holder 11 is inserted into the other nasal cavity of the human body, under the guidance of the white light image displayed on the display 103 Arrive near the nasopharyngeal tissue, conduct optical image observation and spectral collection, then turn on the white light excitation light source, and collect fluorescence imaging, so that the doctor can observe the white light image of the nasopharyngeal tissue under white light illumination, and the doctor finds suspicious in the process of observing the image. When the diseased tissue is detected, the reflection spectrum under white light illumination and the fluorescence spectrum under blue light illumination are collected and observed on the display 103; at the same time, the hand-held probe holder 11 can be in contact with the tissue, and the 785nm Raman excitation light source 3 is turned on to emit laser light. The suspicious tissue is excited by the excitation fiber, and the Raman spectrum signal of the tissue is collected by the collection fiber and transmitted to the Raman spectrometer 2 for detection, realizing the Raman detection of the nasopharynx. Both the optical image and the spectrum signal can be processed by the detector. and real-time saving.

Please refer to FIG. 4 again, the present invention can enter the human body cavity separately from the electronic endoscope 71 to perform multispectral measurement of the tissue in the human body cavity, which can be performed simultaneously and mutually with the current clinical endoscope 71 and pharyngoscopy Together, high-quality multispectral signals of nasopharyngeal tissue can be quickly obtained, and effective detection of nasopharyngeal cancerous tissue can be achieved at the molecular level. For example, when applied to bilateral nostril detection, one side is used to observe the image, and the other side is used to collect Raman signals, which can increase the collection efficiency of Raman light. The hand-held probe 1 of the present invention can not only be used in conjunction with an empty endoscope with a biopsy hole, but also can detect nasopharyngeal tissue together with an endoscope without a biopsy hole or a nasopharyngeal rigid endoscope, and can also be made into a rigid endoscope. . The hand-held probe 1 of the utility model is used in combination with a nasopharyngeal endoscope, does not occupy a biopsy channel, can be combined with the current nasopharyngeal electronic microscope without biopsy, realizes a high degree of integration with clinical nasopharyngoscopy, and has very high clinical application value. The utility model can also be applied to endoscopes 71 with different types of functions, such as nasopharyngoscope, oral mirror, hysteroscope, etc., so as to meet the observation needs of different parts in the human cavity; the hand-held probe can also be applied to the skin outside the human body, For multi-spectral detection such as biopsy tissue, the Raman spectrum detection system has a wide range of wavenumber coverage, and can simultaneously detect Raman spectrum in the fingerprint region and high wavenumber region; it provides an effective clinical detection tool for non-destructive and rapid diagnosis of living tissue.

Claims (9)

1. An endoscopic Raman spectrum detection device for intracavity tissues is characterized in that: the device comprises a handheld probe, a probe support and a fiber-optic probe, wherein the handheld probe is provided with the probe support and the fiber-optic probe; the probe bracket comprises a handheld part and a hollow probe guide pipe, and the handheld part is connected to one end of the probe guide pipe; the head end and the tail end of the probe guide pipe are both of an open structure, one end of the probe guide pipe corresponding to the handheld part is an inlet end, and the other end of the probe guide pipe is a detection end; the optical fiber probe penetrates through the inlet end and is fixed in the probe guide tube.

2. The endoscopic raman spectroscopy apparatus for intraluminal tissue according to claim 1, wherein: the probe guide pipe is provided with a fastening piece corresponding to the inlet end, the optical fiber probe is connected in the probe guide pipe in a sliding mode, and the optical fiber probe is fixed in the probe guide pipe through the fastening piece.

3. The endoscopic raman spectroscopy apparatus for intraluminal tissue according to claim 1, wherein: the probe end of the probe guide pipe is provided with a quartz glass column, and the optical fiber probe is closely connected to the quartz glass column.

4. The endoscopic raman spectroscopy apparatus for intraluminal tissue according to claim 3, wherein: and the front end of the quartz glass column is provided with a fillet.

5. The endoscopic raman spectroscopy apparatus for intraluminal tissue according to claim 1, wherein: the probe holder is made of stainless steel or a rigid, biocompatible material.

6. The endoscopic raman spectroscopy apparatus for intraluminal tissue according to claim 1, wherein: the handheld portion comprises a handle and finger holes, and the finger holes are formed in the lower end of the handle.

7. The endoscopic raman spectroscopy apparatus for intraluminal tissue according to claim 1, wherein: the optical fiber probe comprises a Raman excitation optical fiber, a plurality of Raman collection optical fibers, a fluorescence/reflection excitation optical fiber and a fluorescence/reflection collection optical fiber; the plurality of Raman collection optical fibers enclose a circle; the Raman excitation optical fiber, the fluorescence/reflection excitation optical fiber and the fluorescence/reflection collection optical fiber are arranged in an equilateral triangle and are all positioned in the circle.

8. The endoscopic raman spectroscopy apparatus for intraluminal tissue according to claim 7, wherein: the front end face of the Raman excitation optical fiber is plated with a low-pass film which allows laser with one wavelength to pass through, and the front end of the Raman collection optical fiber is provided with a high-pass filter which can cut off the excitation light and allows Raman scattered light with longer wavelength to pass through.

9. The endoscopic raman spectroscopy apparatus for intraluminal tissue according to claim 7, wherein: the device also comprises a Raman spectrometer, a Raman excitation light source, a white light source, a fluorescence excitation light source, a reflection/fluorescence spectrometer and a detector; the Raman spectrometer is connected with the output ends of the plurality of Raman collecting optical fibers; the output end of the Raman excitation light source is connected with the Raman excitation optical fiber; the white light source and the fluorescence excitation light source are connected with the input end of the fluorescence/reflection excitation optical fiber through a first filter; the reflection/fluorescence spectrometer is connected with the output end of the fluorescence/reflection collection optical fiber through the filter wheel; the Raman spectrometer and the reflection/fluorescence spectrometer are both connected with the detector.

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