CN211122535U - Fluorescence detector - Google Patents
Fluorescence detector Download PDFInfo
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- CN211122535U CN211122535U CN201921568990.3U CN201921568990U CN211122535U CN 211122535 U CN211122535 U CN 211122535U CN 201921568990 U CN201921568990 U CN 201921568990U CN 211122535 U CN211122535 U CN 211122535U
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Abstract
The utility model relates to a fluorescence detector, include: a sample flow cell in which a sample to be detected can be accommodated; a detection device for detecting fluorescence emitted by the sample in response to light; arrange the first optical system in sample flow-through cell upstream, first optical system includes a plurality of emitting diode as the light source, and the light that is sent by a plurality of emitting diode is through the rectangle incident window of sample flow-through cell shines the sample in the sample flow-through cell, so that the sample can send the fluorescence that is used for detecting, and the light that is sent by a plurality of emitting diode is in a plurality of facula that form on the rectangle incident window are followed the long edge direction of rectangle incident window is for fixing a position side by side each other. By means of the fluorescence detector, the optical structure which makes full use of the area of the rectangular incidence window as far as possible to reduce the light intensity loss and properly improve the light intensity irradiating the sample so as to improve the fluorescence quantity of the sample and be beneficial to fluorescence detection can be obtained.
Description
Technical Field
The present invention relates to a fluorescence detector for detecting the fluorescence characteristics emitted by a sample when the sample is irradiated with excitation light, mainly L ED light, which can be used with a liquid chromatograph, a flow injection analyzer, and the like.
Background
Many compounds exhibit photoluminescence. The compound, upon exposure to incident light, absorbs radiant energy and emits characteristic radiation at a wavelength longer than the absorption wavelength. When the incident light ceases to illuminate, the characteristic radiation also disappears very quickly, and this radiation is fluorescence. This radiation may be generated over the entire range of electromagnetic waves. Fluorescence detectors in liquid chromatography use only the fluorescence emitted by absorption of ultraviolet or visible light. A liquid chromatography detector that detects a compound by measuring the intensity of fluorescence of the compound is a fluorescence detector.
Currently, a sample flow cell containing a sample substance to be detected is typically included in a fluorescence detector. Such a sample flow cell may be constructed from a plurality of blocks. For example, the flow cell may be made of different quartz materials that are spliced together and fused at a high temperature, and among these quartz materials, a black quartz material may be used to block light. As schematically shown in fig. 5. In forming the sample flow cell, the structures of the blocks may be used to leave a rectangular entrance window for light illuminating the sample to enter the sample flow cell.
L ED (light emitting diode) is known to be used as a light source to illuminate the sample to cause it to fluoresce in current fluorescence detectors, this illumination is typically done with a single L ED light source, the light from a single L ED reaching the sample flow cell.
When the entrance window is rectangular in shape, on the one hand, when the diameter of the light spot formed by a single L ED on the rectangular entrance window is smaller than the side length of the short side of the rectangle, the light spot is generally far from filling the entire entrance window, and thus a part of the light intensity entering the sample flow cell is lost, whereby the part of the flow cell that is not irradiated is not able to fluoresce, as shown in fig. 3.
On the other hand, if a single L ED forms a spot with a diameter larger than the short side of the rectangle on the rectangular entrance window, it will also result in a loss of part of the light intensity, and thus the light intensity incident on the flow cell will also be smaller and a smaller amount of fluorescence will be generated, as shown in FIG. 4.
For this reason, there is a continuing need for a fluorescence detector that increases the intensity of incident light to increase the amount of fluorescence from the sample and facilitate the detection of fluorescence.
SUMMERY OF THE UTILITY MODEL
The utility model relates to a fluorescence detector, include: a sample flow cell in which a sample to be tested can be accommodated; a detection device for detecting fluorescence emitted by the sample in response to light; a first optical system disposed upstream of the sample flow cell, the first optical system including a plurality of light emitting diodes as a light source, light emitted by the plurality of light emitting diodes being irradiated to the sample in the sample flow cell via a rectangular incidence window of the sample flow cell so that the sample can emit fluorescence for detection, wherein a plurality of light spots formed on the rectangular incidence window by the light emitted by the plurality of light emitting diodes are positioned side by side with each other along a long side direction of the rectangular incidence window.
By means of the fluorescence detector, the optical structure which reduces the light intensity loss by fully utilizing the area of the rectangular incidence window as much as possible and improves the light intensity of the irradiated sample so as to improve the fluorescence quantity of the sample and be beneficial to fluorescence detection can be obtained.
It is particularly preferred that the number of the light emitting diodes is two, and the diameters of two light spots formed on the rectangular incidence window by the light emitted from the two light emitting diodes are the same.
Since more leds make the arrangement of the optics in the first optical system more difficult and complicated, the "double L ED configuration" is the best solution for a conventional rectangular entrance window to balance the design, arrangement, and manufacturing aspects.
In a preferred embodiment, the long side of the rectangular entrance window is twice as long as the short side, and the diameter of the light spot is equal to the length of the short side of the rectangular entrance window. Therefore, the two light spots formed on the rectangular incidence window can utilize the area of the window to the maximum extent so as to improve the light intensity and reduce the loss to the maximum extent.
Furthermore, the first optical system may further comprise a mirror arranged facing the sample flow cell, via which mirror light emitted by the two light emitting diodes is diverted to the rectangular entrance window of the sample flow cell.
By means of this mirror, the light emitted by the two light-emitting diodes can be diverted, so that a flexible arrangement of the light-emitting diodes as light sources, in particular of a plurality of light-emitting diodes, in the first optical system and also in the entire fluorescence detector is facilitated.
In particular, two light-emitting diodes are arranged on opposite sides of the mirror, so that the arrangement is simple (symmetrical arrangement) and the light intensities of the two beams of light acting on the sample remain as uniform as possible.
Further, the first optical system may further include two first condensing lenses respectively disposed between the reflecting mirror and each of the two light emitting diodes. Thereby, light from the light emitting diode can be focused onto the mirror to facilitate the formation of a spot of a desired diameter on the rectangular entrance window of the sample flow cell.
In particular, the first optical system may further comprise a first filter arranged between each first focusing lens and each of the two light emitting diodes, such that the filtered light can subsequently reach the sample flow cell.
In addition, the fluorescence detector according to the present invention may further include a second optical system disposed between the sample flow cell and the detection device to guide fluorescence emitted from the sample to the detection device for fluorescence detection. With the second optical system, the fluorescence detection efficiency can be improved by adjusting the fluorescence optical path.
In some embodiments, the second optical system may include a second focusing lens for focusing the fluorescent light emitted by the sample to form a fluorescent spot of a desired size on the detection device.
In addition, the second optical system may further include a second filter located between the second focusing lens and the detection device, and the fluorescence reaches the detection device after being filtered by the second filter. This can improve the accuracy of fluorescence detection.
Drawings
FIG. 1 schematically illustrates a general layout of a fluorescence detector according to one embodiment of the present invention, showing light propagation paths;
FIG. 2 schematically shows spots of light emitted by two light emitting diodes formed on a rectangular entrance window of a fluorescence detector according to the embodiment of the invention in FIG. 1, wherein the diameter of each spot corresponds to the length of the short side of the rectangular entrance window;
FIG. 4 schematically illustrates a spot formed on a rectangular entrance window by a single L ED of a prior art fluorescent detector, wherein the diameter of the spot is too large such that light intensity beyond the rectangular entrance window is lost;
fig. 5 schematically illustrates a perspective view of a sample flow cell of a fluorescence detector according to an embodiment of the present invention.
List of reference numerals:
1 a light emitting diode;
2 a first optical filter;
3 a first converging lens;
4, a reflector;
5 a light emitting diode;
6 a first optical filter;
7 a first converging lens;
8 a mirror;
9 a sample flow cell;
10 a second converging lens;
11 a second optical filter;
12 a detection device;
13,14 light spots;
100 fluorescence detector.
Detailed Description
It should be noted that the drawings referred to are not all drawn to scale but may be exaggerated to illustrate various aspects of the present invention, and in this regard, the drawings should not be construed as limiting.
The fluorescence detector according to the present invention can be used for various biological or chemical apparatuses such as a liquid chromatograph. Fluorescence detectors typically include a sample flow cell. Generally, a rectangular cell made of quartz glass or the like is used as the sample cell. However, a flow cell may be used when the detector uses liquid chromatography or a flow injection analyzer. In the present invention, a "sample cell" and a "flow cell" are not strictly distinguished, the term "sample flow cell" referring to any suitable container or element in which the sample to be detected is contained.
According to the utility model discloses a sample flow-through cell includes the rectangle incident window. Here, the term "rectangular" means a square shape including a difference in the length of the long side and the short side, wherein the length of the long side is larger than that of the short side. In some particular cases, however, the entrance window of the sample flow cell may not be exactly rectangular in shape, but rather may be substantially rectangular.
In particular, the light source is a light emitting diode L ED which emits light at a wavelength suitable for causing the sample in the sample flow cell 9 to emit fluorescence to be detected.
In the present invention, the fluorescence detector 100 includes a detection device 12 for detecting fluorescence excited in a sample in response to light irradiation by a light emitting diode (the direction of fluorescence to be detected emitted from a sample flow cell is exemplarily shown by an arrow in fig. 5). Since the design of the detection device 12 is known per se, it is not described in detail here.
In accordance with the present invention, the light emitted from the light emitting diode L ED of the fluorescence detector 100 as a light source can reach the rectangular incident window of the sample flow cell 9 and form a light spot thereon, rather than a single light emitting diode, the energy of the enhanced fluorescence can increase the sensitivity of the fluorescence detector 100, while the intensity of the enhanced excitation light can increase the intensity of the generated fluorescence.
In order to make the facula on the one hand on the rectangle incident window not too big in order to lose its light intensity and also not too little thereby can't utilize the whole area of rectangle incident window, according to the utility model discloses a design, a plurality of faculas that the light that sends by a plurality of emitting diode formed on the rectangle incident window are for fixing a position side by side each other along the long edge direction of rectangle incident window. Here, the term "side by side" means that a plurality of spots are arranged in the long side direction of the rectangular incidence window, and the central connecting line of the spots is extended in the long side direction of the rectangular incidence window.
Advantageously, the spots on the rectangular entrance window are adjacent to each other but do not overlap as much as possible. It will be appreciated that a small degree of overlap is not entirely impossible, as the intensity at the edges of the spots is generally weak and a small amount of overlap does not significantly affect the intensity received by the sample in the sample flow cell 9. However, it is more advantageous if the arrangement of a plurality of light spots on a rectangular entrance window is exactly adjacent to one another, since the area of the light spots can then be used to cover the rectangular entrance window.
In a preferred embodiment, the number of LEDs may be two, and this optical configuration may be referred to herein as the "double L ED configuration" of the fluorescence detector 100. on the one hand, the double L ED light spots 13 and 14 may take full advantage of the area of the entrance window and the intensity of the two LEDs 1, 5. on the other hand, more LEDs (e.g., each forming a smaller light spot) may make the placement of optics within the first optical system more difficult and complex.
It is particularly advantageous if the diameters of the two light spots formed by the light emitted by the two light-emitting diodes 1,5 on the rectangular entrance window are identical or approximately identical. On the one hand, the arrangement of the light source can be more universal, and on the other hand, the area of the rectangular incidence window can be fully utilized to increase the light intensity.
In the embodiment shown in fig. 2, the long side of the rectangular entrance window is twice as long as the short side, and the diameter of the spot is equal to the length of the short side of the rectangular entrance window. In this case, two light spots with the same size can occupy the area of the rectangular incidence window to the maximum extent, thereby improving the light intensity of the irradiated sample. It will be appreciated that the dimensions of the long and short sides of the rectangular entrance window need not be so dimensioned, but may also be in other aspect ratios. Under the condition that a plurality of light emitting diodes are arranged side by side along the long side of the rectangular incidence window, the diameter size of a light spot formed by the plurality of light emitting diodes and the number of the light emitting diodes can be selected by integrating the arrangement difficulty of the whole light path and the light intensity required by fluorescence detection.
Turning again to fig. 1, the first optical system according to the present invention further comprises a mirror arranged facing the sample flow cell 9, in particular facing the rectangular entrance window. The mirror should be spaced a suitable distance from the sample flow cell 9. The light emitted by the two light emitting diodes 1,5 is diverted via this mirror (typically one mirror) to a rectangular entrance window of the sample flow cell 9. In other words, the reflector serves to divert the light emitted by the two light-emitting diodes 1,5, so that a flexible arrangement of the light-emitting diodes as light sources, in particular of a plurality of light-emitting diodes, in the first optical system and also in the entire fluorescence detector 100 is facilitated.
For example, the mirror may be constructed from two sub-mirrors that are at an angle (e.g., right angle) to each other, as shown in FIG. 1. Light rays from each of a plurality, e.g. two, of the light emitting diodes 1,5 may be turned through an angle (e.g. 90 degrees) via the mirror. It is particularly advantageous that the plurality of light emitting diodes are arranged symmetrically with respect to the reflector, for example two light emitting diodes 1,5 are arranged on opposite sides of the reflector as shown in fig. 1.
Furthermore, the first optical system according to the present invention may further comprise a corresponding plurality, in particular two, first converging lenses 3, 7. A first condensing lens is arranged between the mirror and each of the two light emitting diodes 1,5, respectively, to condense light from the light emitting diodes onto the mirror to subsequently form a spot of a desired diameter on the rectangular entrance window.
In this embodiment, the first optical system may further include a first filter (e.g., 4 or 8 in fig. 1) disposed between each first focusing lens and each of the plurality of light emitting diodes such that the filtered light can subsequently reach the sample flow cell 9.
On the other hand, the fluorescence detector 100 may further include a second optical system arranged between the sample flow cell 9 and the detection device 12 in addition to the first optical system located upstream of the sample flow cell 9 to guide fluorescence emitted from the sample onto the detection device 12 for fluorescence detection.
Preferably, as shown in fig. 2, the second optical system may similarly include a second condensing lens 10 for condensing fluorescence emitted from the sample. In addition, the second optical system may further include a second optical filter 11 located between the second focusing lens 10 and the detection device 12, and the fluorescence reaches the detection device 12 after being filtered by the second optical filter 11, so as to improve the fluorescence detection performance.
Finally, in addition to the various optical devices described above, the present invention may also include means for limiting the light beam, fluorescence adjusting means for changing the fluorescence, light condensing means (for example, since the fluorescence passing through the aperture is efficiently guided to the detecting means 12 by the light condensing means, the amount of fluorescence incident on the detecting means 12 is not significantly reduced even compared to the case where the aperture length is large, which allows measurement with sufficient sensitivity), other mirrors or refractors, and the like.
It will be appreciated that if the number of LEDs is greater than two, e.g., four, eight, then the arrangement of the various optics, e.g., converging lenses, mirrors, etc., in the first optical system can create certain difficulties and still ensure that there is no interference between the various optical paths.
Claims (10)
1. A fluorescence detector (100) comprising:
a sample flow cell (9) inside which a sample to be tested can be accommodated;
a detection device (12) for detecting fluorescence emitted by the sample in response to light;
characterized in that the fluorescence detector (100) further comprises:
a first optical system arranged upstream of the sample flow cell (9), the first optical system including a plurality of light emitting diodes as a light source, light emitted by the plurality of light emitting diodes being irradiated to the sample in the sample flow cell (9) via a rectangular incidence window of the sample flow cell (9) so that the sample can emit fluorescence for detection, wherein a plurality of light spots formed on the rectangular incidence window by the light emitted by the plurality of light emitting diodes are positioned side by side with each other along a long side direction of the rectangular incidence window.
2. The fluorescence detector (100) according to claim 1, wherein the number of the plurality of light emitting diodes is two and the diameters of two light spots (13,14) formed on the rectangular entrance window by the light emitted by the two light emitting diodes (1,5) are the same.
3. A fluorescence detector (100) according to claim 2, wherein the long side of the rectangular entrance window is twice as long as the short side and the diameter of the spot (13,14) is equal to the length of the short side of the rectangular entrance window.
4. The fluorescence detector (100) according to claim 2, wherein the first optical system further comprises a mirror (4,8) arranged facing the sample flow cell (9), light emitted by the two light emitting diodes (1,5) being diverted via the mirror (4,8) to the rectangular entrance window of the sample flow cell (9).
5. The fluorescence detector (100) according to claim 4, wherein the two light emitting diodes (1,5) are arranged on opposite sides of the mirror (4, 8).
6. The fluorescence detector (100) according to claim 4, wherein the first optical system further comprises two first converging lenses (3,7), the first converging lenses (3,7) being arranged between the mirror (4,8) and each of the two light emitting diodes (1,5), respectively.
7. The fluorescence detector (100) according to claim 5, wherein the first optical system further comprises a first filter (2,6) arranged between each first converging lens (3,7) and each of the two light emitting diodes (1, 5).
8. The fluorescence detector (100) according to any of claims 1-7, wherein the fluorescence detector (100) further comprises a second optical system arranged between the sample flow cell (9) and the detection means (12) to guide fluorescence emitted by the sample onto the detection means (12) for fluorescence detection.
9. The fluorescence detector (100) according to claim 8, wherein the second optical system comprises a second converging lens (10) for converging fluorescence light emitted by the sample.
10. The fluorescence detector (100) according to claim 9, wherein the second optical system further comprises a second filter (11) between the second converging lens (10) and the detection means (12), the fluorescence reaching the detection means (12) after filtering by the second filter (11).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921568990.3U CN211122535U (en) | 2019-09-20 | 2019-09-20 | Fluorescence detector |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921568990.3U CN211122535U (en) | 2019-09-20 | 2019-09-20 | Fluorescence detector |
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| Publication Number | Publication Date |
|---|---|
| CN211122535U true CN211122535U (en) | 2020-07-28 |
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| CN201921568990.3U Active CN211122535U (en) | 2019-09-20 | 2019-09-20 | Fluorescence detector |
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| CN (1) | CN211122535U (en) |
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2019
- 2019-09-20 CN CN201921568990.3U patent/CN211122535U/en active Active
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