CN210982226U - Magnetic bead separation and cleaning device with mixed structure - Google Patents

Abstract

The utility model provides a take mixed structure's magnetic bead separation and belt cleaning device, including the installation component, magnetic bead adsorption equipment, and the washing dish part that from the bottom up arranged in proper order of setting on the installation component, mixing device and needle subassembly, wash the dish part including by the rotatory annular washing dish of drive and install a plurality of reaction cups on wasing the dish, the needle subassembly includes lift needle frame and installs a plurality of needles on lift needle frame, mixing device includes mixing plate and a plurality of mixing subassemblies of vertical mounting on mixing plate, the needle gets into the reaction cup after passing mixing plate and/or mixing subassembly perpendicularly, the mixing subassembly includes the mixing stick, mixing stick center pin and reaction cup center pin are parallel and lie in the reaction cup outside, mixing stick one end is equipped with the arch, the mixing stick is driven rotatory, it supports and drags the reaction cup to drive protruding spaced. The utility model aims at providing a make the magnetic bead separation and belt cleaning device of abundant, the simple reliable mixed structure of taking mixed structure of reaction liquid mixing.

Description

Magnetic bead separation and cleaning device with mixed structure

Technical Field

The utility model relates to the field of medical equipment, specifically be a take mixed structure's magnetic bead separation and belt cleaning device.

Background

In chemiluminescence immunoassay, a magnetic particle compound is a carrier of a signal to be detected. The magnetic separation technology is to carry out solid-liquid phase separation on the magnetic particle compound and the reaction liquid. In order to ensure the effect of magnetic separation, the reaction solution needs to be fully mixed in the separation process. Thereby obtaining an accurate test result. In the prior art, a contact type pulp sheet uniformly mixing structure and a suction liquid beating uniformly mixing structure are mainly adopted.

In automatic detection devices, a mixing mechanism and a stirring rod are generally used to stir the solution in one reactor at a time. However, the kneading mechanism of this detection apparatus has a long kneading operation execution cycle, and in order to avoid the problem of mutual contamination of the reaction liquids, it is necessary to execute a cleaning operation, and the cycle is long.

In current full-automatic immunity luminescence analysis appearance, adopt the magnetic bead to adsorb the mixing structural style of extraction waste liquid and beat the washing liquid mostly, the reaction cup need be through other auxiliary device round trip movement between two positions, the flow is more complicated, is not suitable for automatic cleaning, and the mixing is not abundant.

SUMMERY OF THE UTILITY MODEL

To the above-mentioned problem that prior art exists, the utility model aims at providing a make the magnetic bead separation and the belt cleaning device of taking mixed structure that the reaction liquid mixing is abundant, mixing simple structure is reliable.

In order to realize the purpose, the utility model discloses the technical scheme who adopts is:

The utility model provides a take mixed structure's magnetic bead separation and belt cleaning device, including the installation component, magnetic bead adsorption equipment, and the washing dish part that from the bottom up arranged in proper order of setting on the installation component, mixing device and needle subassembly, washing dish part is including being driven rotatory annular washing dish and installing a plurality of reaction cups on wasing the dish, the needle subassembly includes lift needle frame and installs a plurality of needles on lift needle frame, mixing device includes mixing plate and a plurality of mixing subassembly of vertical installation on mixing plate, the needle passes perpendicularly and gets into the reaction cup behind mixing plate and/or the mixing subassembly, the mixing subassembly includes the mixing stick, the parallel reaction cup that just is located of mixing stick center pin and reaction cup inside, mixing stick one end is equipped with the arch, the mixing stick is driven rotatory, it supports and drags the reaction cup to drive protruding spaced.

The further improvement of the technical scheme is as follows:

The blending component also comprises a blending shaft and a bearing, the blending shaft passes through the blending plate and is sleeved outside the blending rod in a co-motion manner, and the two sides of the bearing are respectively connected with the blending shaft and the blending plate.

The mixing device further comprises a direct current motor and a synchronous belt, the direct current motor is arranged on the mixing plate, the synchronous belt is located below the mixing plate, and the two ends of the synchronous belt are respectively connected with the direct current motor and the mixing shaft.

The blending assembly further comprises a synchronous wheel, the synchronous wheel is sleeved outside the blending shaft below the blending plate, and the synchronous wheel is connected with a synchronous belt.

The mounting component comprises a mounting plate and a lifting base arranged on the mounting plate, the cleaning disc component is arranged on the mounting plate, and the blending device and the needle assembly are arranged on the lifting base in a lifting way.

The blending device and the needle assembly are respectively connected with a screw motor.

The cleaning disc component also comprises a cleaning disc mounting frame fixed on the mounting plate, and the cleaning disc is rotatably arranged on the cleaning disc mounting frame.

The cleaning disc component also comprises a stepping motor which is connected with and drives the cleaning disc to rotate in a stepping mode.

The magnetic bead adsorption device comprises a plurality of magnet mounting frames and a plurality of magnets, the magnet mounting frames are fixed on the cleaning disc mounting frames at intervals, and a plurality of magnets are arranged on each cleaning disc mounting frame at intervals.

The needles include a liquid suction needle for sucking waste liquid, a cleaning needle for injecting a cleaning liquid, and a substrate needle for injecting a substrate.

Compared with the prior art, the beneficial effects of the utility model are that: the mixing rod does not contact the reaction liquid in the reaction cup, so that cross contamination is avoided; one end of the mixing rod is provided with a bulge which drives the reaction cup to rotate and vibrate simultaneously, so that the reaction liquid is fully mixed, and meanwhile, the mixing rod has a simple and reliable structure and low cost; reaction liquid in a plurality of reaction cups can be operated simultaneously, the execution period of the uniform mixing action is greatly shortened, and the flow is simple; the compact direct current motor with single-side output drives the blending device, and has compact volume and small structure.

Drawings

Fig. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic structural view of the cleaning disc member and the magnetic bead adsorption device of the present invention;

FIG. 3 is a schematic structural view of the blending device of the present invention;

Fig. 4 is the structure diagram of the blending component of the present invention.

Detailed Description

The magnetic bead separating and washing device with the hybrid structure according to the present invention will be described in detail and fully with reference to the following embodiments. The following description of the embodiments is merely exemplary in nature and is in no way intended to limit the invention.

A magnetic bead separating and cleaning device with a mixing structure comprises a mounting component, and a cleaning disc component, a blending device 4 and a needle component which are arranged on the mounting component in sequence from bottom to top, as shown in figures 1-4.

The mounting component comprises a mounting plate 11 and a lifting base 12 arranged on the mounting plate 11, the cleaning disc component is arranged on the mounting plate 11, and the blending device 4 and the needle assembly are arranged on the lifting base 12 in a lifting mode.

The cleaning disk unit is shown in FIG. 2 and includes a cleaning disk mounting frame, a cleaning disk 21, a stepping motor 23 and a plurality of reaction cups 22. The cleaning disc mounting frame is fixed on the mounting plate 11, the cleaning disc 21 is annular, the cleaning disc 21 is rotatably mounted on the cleaning disc mounting frame, and specifically, the cleaning disc 21 is driven by the stepping motor 23 to rotate by taking the central line of the cleaning disc as a central shaft. The above-mentioned rotation of the cleaning disc 21 is well known to those skilled in the art and will not be described herein. The plurality of reaction cups 22 are mounted on the cleaning disk 21 in parallel and at regular intervals, and the height direction of the reaction cups 22 is parallel to the central axis direction of the cleaning disk 21. Specifically, a plurality of cup positions are uniformly arranged on the cleaning disc 21 at intervals, and each reaction cup 22 is placed on one cup position. The stepping motor 23 is located on the mounting plate 11, and the stepping motor 23 is connected with and drives the cleaning disc 21 to rotate in a stepping manner, and simultaneously drives the reaction cups 22 to move synchronously. Specifically, the cleaning disc 21 rotates around its central axis, and the span of each step-by-step rotation of the cleaning disc 21 is the distance between two reaction cups 22, i.e. after each rotation, with respect to the position of the cleaning disc mounting frame, the spatial position of one reaction cup 22 before the rotation is replaced by the adjacent reaction cup 22. The cleaning disk 21 is rotated counterclockwise by one position of the cuvette 22 every fixed period to provide time for cleaning and the like. Preferably, 30 cup positions are arranged, that is, 30 reaction cups 22 can be placed at the same time for operation.

Separation and belt cleaning device still includes magnetic bead adsorption equipment 3, and magnetic bead adsorption equipment 3 includes a plurality of magnet mounting bracket 31 and a plurality of magnet. A plurality of magnet mounting brackets 31 are fixed on the cleaning disc mounting bracket at intervals, and a plurality of magnets are arranged on each magnet mounting bracket 31 at intervals. The magnet mounting bracket 31 is located outside the arc-shaped side surface of the cleaning disc 21. Preferably, there are 4 magnet mounting frames 31, three magnets are uniformly spaced on each magnet mounting frame 31, the three magnets of each magnet mounting frame 31 correspond to three consecutive cup positions on the cleaning disc 21 in turn, and there are 12 magnets, each magnet corresponds to one of the cup positions, that is, to one reaction cup 22, in other words, when viewed from the annular end surface of the cleaning disc 21, the line connecting each magnet and the corresponding reaction cup 22 coincides with the radius of the annular shape. The arrangement is such that the magnet can adsorb the magnetic beads in the reaction cup 22. The magnet is a strong rubidium magnet.

As shown in fig. 3, the kneading apparatus 4 includes a kneading plate 41, a dc motor 43, a timing belt 44, and a plurality of kneading units 42. The blending plate 41 is arranged on the lifting base 12 in a lifting way, and the blending plate 41 and the mounting plate 11 are arranged in parallel at intervals. Specifically, the lifting base 12 is provided with a guide rod, the guide rod is perpendicular to the mounting plate 11, and the guide rod penetrates through the blending plate 41. The blending plate 41 is further connected with a screw motor, and the screw motor is connected with and drives the blending plate 41 to move along the guide rod in the direction perpendicular to the mounting plate 111. The lead screw motor includes the motor, lead screw and nut, and the lead screw is the straight line type, and the lead screw surface is equipped with the spiral groove, and the nut cup joints at the outside and lead screw meshing of lead screw, and mixing board 41 is still connected to the nut, and the lead screw is connected to the motor, and motor drive lead screw is rotatory, drives the nut along lead screw axial displacement, changes the rotary motion of lead screw into the linear motion of nut promptly, consequently drives mixing board 41 and moves on the straight line of perpendicular to mounting panel 11. The principle and construction of the lead screw motor are well known to those skilled in the art and will not be described in detail herein.

Direct current motor 43, hold-in range 44 and a plurality of mixing subassembly 42 are all installed on mixing board 41, and mixing subassembly 42 passes mixing board 41 perpendicularly, and hold-in range 44 is located mixing board 41 below, and direct current motor 43 and mixing subassembly 42 are connected respectively to hold-in range 44 both ends, and direct current motor 43 passes through hold-in range 44 drive mixing subassembly 42 and rotates. Preferably, four blending assemblies 42 are provided, and the blending assemblies 42 are driven by the dc motor 43 and the synchronous belt 44 to rotate at regular time.

The blend assembly 42 is shown in FIG. 4 and includes a blend bar 421, a blend shaft 422, bearings 424 and a synchronizing wheel 426. The blending shaft 422 passes through the blending plate 41 and is sleeved outside the blending rod 421 in a co-motion manner, two sides of the bearing 424 are respectively connected with the blending shaft 422 and the blending plate 41, namely, the blending shaft 422 and the blending plate 41 are connected through the bearing 424 to realize relative rotation with the blending plate 41, and preferably, the bearing 424 is a deep groove ball bearing. The upper end and the lower end of the bearing 424 are provided with a circlip for hole 423 and a circlip for shaft 425, respectively, and are located in a gap between the kneading shaft 422 and the kneading plate 41. The synchronizing wheel 426 is sleeved outside the blending shaft 422 below the blending plate 41 and contacts with the blending shaft 422, the synchronizing wheel 426 is connected with the synchronizing belt 44, namely, two ends of the synchronizing belt 44 are respectively connected with the direct current motor 43 and the synchronizing wheel 426, and the direct current motor 43 drives the blending shaft 422 to rotate through the synchronizing belt 44 and the synchronizing wheel 426. The mixing rod 421 is T-shaped, the horizontal part of the T-shape is located above the mixing plate 41 and connected to one end of the mixing shaft 422, the vertical part of the T-shape is cylindrical, and the vertical part passes through and protrudes out of the mixing shaft 422. Mixing stick 421 center pin and reaction cup 22 center pin are parallel and are located the reaction cup 22 outside, and the vertical part of mixing stick 421 passes mixing axle 422's one end lateral surface and is equipped with the arch, and mixing stick 421 is driven rotatoryly, and protruding spaced supports to lean on and drags reaction cup 22, makes reaction cup 22 both produce rotary motion, produces the shock motion again to do benefit to the abundant mixing of magnetic bead and washing liquid in the reaction cup 22. Place reaction cup 22 cup position internal diameter is greater than reaction cup 22's external diameter, when reaction cup 22 placed naturally, the protruding contactless reaction cup 22 of mixing stick 421, the non-bellied cylinder portion of mixing stick 421 is less than the height of protruding mixing stick 421 with reaction cup 22's distance this moment, namely, when mixing stick 421 rotates to protruding contact reaction cup 22, because the effect of the friction force between arch and the reaction cup 22, the arch drives reaction cup 22 and rotates, and simultaneously, because the relation of the above-mentioned distance of setting for, the arch can be towards the direction of pushing away reaction cup 22 with mixing stick 421 vertically, make reaction cup 22 vibrate. The kneading plate 41 and the kneading shaft 422 are also provided with a plurality of through holes for the passage of needle assemblies, as will be described in detail below.

The needle assembly includes an elevating needle frame 51 and a plurality of needles mounted on the elevating needle frame 51. The needle lifting frame 51 is arranged on the lifting base 12 in a lifting manner, the needle lifting frame 51 penetrates through another guide rod on the lifting base 12, the needle lifting frame 51 is connected with another screw motor, the screw motor drives the needle lifting frame 51 to move along a direction perpendicular to the mounting plate 11, the lifting principle of the needle lifting frame is the same as that of the blending plate 41, and the description is omitted. The needles include a pipette needle for aspirating waste liquid in the reaction cup 22, a wash needle for injecting a wash liquid into the reaction cup 22, and a substrate needle for injecting a substrate liquid into the reaction cup 22. The liquid suction needle, the cleaning needle and the substrate needle are vertical to the mounting plate 11. Preferably, there are four pipette needles (7a, 7c, 7e, 7g), three wash needles (7b, 7d, 7f) and one substrate needle (7 h). The outer diameters of the through holes on the blending plate 41 and the blending shaft 422 are larger than the outer diameters of the liquid suction needle, the cleaning needle or the substrate needle, and the liquid suction needle, the cleaning needle and the substrate needle penetrate through the through holes and then enter the reaction cup 22 to suck or inject liquid. That is, the connecting line of the projections of the eight needles on the mounting plate 11 is an arc, and the radius of the arc is equal to the radius of the circle projected by the reaction cup 22 on the mounting plate 11.

The needle, plurality of blending assemblies 42, and plurality of magnets are positioned relative to one another. When facing the mounting plate 11, the needles are arranged in the counterclockwise direction as a pipette needle 7a, a cleaning needle 7b, a pipette needle 7c, a cleaning needle 7d, a pipette needle 7e, a cleaning needle 7f, and a pipette needle 7g, that is, the pipette needle and the cleaning needle are arranged at an interval. Two corresponding cup positions of the liquid suction needle 7a and the cleaning needle 7b are adjacent; two cup positions are arranged between the two corresponding cup positions of the cleaning needle 7b and the liquid suction needle 7c respectively; two corresponding cup positions of the liquid suction needle 7c and the cleaning needle 7d are adjacent; two cup positions are arranged between the two corresponding cup positions of the cleaning needle 7d and the liquid suction needle 7e respectively; two corresponding cup positions of the liquid suction needle 7e and the cleaning needle 7f are adjacent; two cup positions are arranged between the two corresponding cup positions of the cleaning needle 7f and the liquid suction needle 7g respectively.

The magnets are not provided outside the cup positions corresponding to the three cleaning needles (7b, 7d, 7 f). The cup positions with the magnets are as follows: the cup position corresponding to the liquid suction needle 7a, the two cups on one side of the cup position corresponding to the liquid suction needle 7a are positioned on the same magnet mounting frame 31; two cup positions between two corresponding cup positions of the cleaning needle 7b and the liquid suction needle 7c respectively and a cup position corresponding to the liquid suction needle 7c are positioned on the same magnet mounting frame 31; two cup positions between two corresponding cup positions of the cleaning needle 7d and the liquid suction needle 7e respectively and a cup position corresponding to the liquid suction needle 7e are positioned on the same magnet mounting frame 31; two cup positions between two corresponding cup positions of the cleaning needle 7f and the liquid suction needle 7g respectively and a cup position corresponding to the liquid suction needle 7g are positioned on the same magnet mounting frame 31. A total of twelve magnets.

The blending assemblies 42 correspond to a cup position corresponding to the substrate needle 7h, a cup position corresponding to the cleaning needle 7f, a cup position corresponding to the cleaning needle 7d, and a cup position corresponding to the cleaning needle 7b, that is, four cup positions in total. The mixing rod 421 of the mixing component 42 is used for mixing the corresponding cup position.

Four aspirating needles (7a, 7c, 7e, 7g), three cleaning needles (7b, 7d, 7f) and one substrate needle (7h) respectively penetrate through the mixing plate 41 and/or the mixing shaft 422.

The operation principle and process of the present invention will be described by taking the movement of a target cuvette 22 as an example: the working principle is as follows: the blending plate 41 and the lifting needle frame 51 move up and down synchronously, the blending plate 41 drives the blending component 42 to move, and the lifting needle frame 51 drives a plurality of needles to move. In other words, the plurality of blending assemblies 42 and the plurality of needles move up and down synchronously, the plurality of needles being: four pipette needles (7a, 7c, 7e, 7g), three cleaning needles (7b, 7d, 7f) and one substrate needle (7 h). When the plurality of blending assemblies 42 and the plurality of needles move downwards, four liquid suction needles (7a, 7c, 7e and 7g), three cleaning needles (7b, 7d and 7f) and one substrate needle (7h) extend into the reaction cup 22 in the corresponding cup position, and the blending rods 421 of the plurality of blending assemblies 42 are positioned outside the corresponding reaction cup 22; when the plurality of kneading modules 42 and the plurality of needles are moved upward, they are separated from the respective reaction cups 22 and positioned above the respective reaction cups 22 so as not to block the rotation of the washing tray 21 and the reaction cups 22.

The working process is as follows: from the upper side of the elevating needle frame 51 to the cleaning disk 21, the stepping motor 23 drives the cleaning disk 21 to rotate counterclockwise in steps, and the span of each rotation is the distance between two cup positions.

The first step is as follows: the target cuvette 22 enters the washing tray 21 and is driven to rotate through the set of magnet mounting bracket 31, the magnet adsorbs the magnetic beads, and the pipette needle 7a sucks out waste liquid.

The outside manipulator puts into the cup position on the washing dish 21 with the target reaction cup 22 that is equipped with waste liquid and the mixed liquid of magnetic bead, it is rotatory that washing dish 21 is driven, when target reaction cup 22 reachs two cup positions that imbibition needle 7a corresponds cup position one side, the magnetic bead is adsorbed by the outside magnet of corresponding cup position, when target reaction cup 22 is located the cup position that imbibition needle 7a corresponds, mixing plate 41 and lift needle frame 51 are by the synchronous downward removal of the lead screw motor drive that corresponds separately, drive a plurality of mixing subassemblies 42 and the synchronous downward removal of many needles, imbibition needle 7a stretches into in the target reaction cup 22, the waste liquid in the imbibition cup. Then, the kneading plate 41 and the lift pin holder 51 move upward, and the stepping motor 23 drives the cleaning disk 21 to rotate continuously.

The second step is that: after the target reaction cup 22 is filled with the cleaning solution and mixed uniformly, the magnet adsorbs the magnetic beads when the target reaction cup 22 passes through the magnet mounting rack 31, and finally the liquid absorption needle absorbs the waste liquid.

Injecting a cleaning solution into the target reaction cup 22 and mixing uniformly: target reaction cup 22 arrives and washs needle 7b and corresponds the cup position, and mixing subassembly 42 and many synchronous down movements of needle wash needle 7b, wash in needle 7b stretches into target reaction cup 22, pour into the washing liquid into in reaction cup 22, simultaneously mixing rod 421 of mixing subassembly 42 that the cup position corresponds is located target reaction cup 22 outside, and direct current motor 43 drives mixing rod 421 rotatory, and mixing rod 421's protruding periodic contact target reaction cup 22 drags target reaction cup 22 rotatory, and the direction that promotes reaction cup 22 simultaneously and remove towards perpendicular to mixing rod 421 realizes target reaction cup 22's rotation and vibration, makes washing liquid and the magnetic bead mix in the target reaction cup 22, fully washs the magnetic bead.

The magnetic beads are adsorbed by the magnets when the target reaction cup 22 passes through the magnet mounting frame 31: the blending plate 41 and the lifting needle frame 51 move upwards, the stepping motor 23 drives the cleaning disc 21 to rotate anticlockwise to drive the target reaction cup 22 to rotate three cup positions, and when the target reaction cup passes through the three cup positions, magnetic beads in the target reaction cup 22 are adsorbed by magnets outside the three cup positions.

The liquid suction needle sucks waste liquid: when the target reaction cup 22 rotates to the third cup position, the target reaction cup enters the cup position corresponding to the liquid suction needle 7c, the blending component 42 and the plurality of needles synchronously move downwards, and the liquid suction needle 7c enters the target reaction cup 22 to suck waste liquid.

Repeating the second step twice, wherein when repeating the first step, the cleaning needle 7d injects cleaning solution into the target reaction cup 22 and mixes the cleaning solution, the magnet adsorbs magnetic beads, and the liquid suction needle 7e sucks out waste liquid; in the second repetition, the washing needle 7f injects and mixes the washing solution into the target reaction cup 22, the magnet adsorbs the magnetic beads, and the pipette needle 7g aspirates the waste liquid.

Thus, the magnetic beads in the target cuvette 22 are washed by injecting the washing solution three times.

The third step: after injecting the substrate into the target reaction cup 22, the target reaction cup 22 is removed from the apparatus.

When the target reaction cup 22 passes through the cup position corresponding to the liquid suction needle 7g, the waste liquid in the target reaction cup 22 is sucked out, and the magnetic beads are washed for three times and can be reinjected into the substrate for detection. At this time, the target reaction cup 22 reaches the cup position corresponding to the substrate needle 7h, the substrate needle 7h injects the substrate into the target reaction cup 22, the substrate is simultaneously driven by the blending assembly 42 to be blended, the cleaning disc 21 continues to rotate, and the target reaction cup 22 with the substrate is taken out by the manipulator and enters other detection lines again.

Finally, it must be said here that: the above embodiments are only used for further detailed description of the technical solutions of the present invention, and should not be understood as limiting the scope of the present invention, and the modifications and adjustments made by those skilled in the art according to the above-mentioned contents of the present invention are all included in the scope of the present invention.

Claims (10)

1. The utility model provides a take magnetic bead separation and belt cleaning device of mixed structure, including the installation component, magnetic bead adsorption equipment (3), and the washing plate part that from the bottom up arranged in proper order of setting on the installation component, mixing device (4) and needle subassembly, the washing plate part includes by drive rotatory annular washing dish (21) and install a plurality of reaction cup (22) on washing dish (21), the needle subassembly includes lift needle frame (51) and installs a plurality of needles on lift needle frame (51), a serial communication port, mixing device (4) include mixing board (41) and install a plurality of mixing subassembly (42) on mixing board (41) perpendicularly, the needle passes mixing board (41) and/or mixing subassembly (42) perpendicularly and then gets into reaction cup (22), mixing subassembly (42) include mixing stick (421), mixing stick (421) center pin and reaction cup (22) center pin are parallel and lie in reaction cup (22) outside, one end of the mixing rod (421) is provided with a bulge, and the mixing rod (421) is driven to rotate to drive the bulge to lean against and drag the reaction cup (22) at intervals.

2. The magnetic bead separation and washing apparatus with a mixing structure according to claim 1, wherein: the blending component (42) also comprises a blending shaft (422) and a bearing (424), the blending shaft (422) penetrates through the blending plate (41) and is sleeved outside the blending rod (421) in a co-motion manner, and two sides of the bearing (424) are respectively connected with the blending shaft (422) and the blending plate (41).

3. The magnetic bead separation and washing apparatus with a mixing structure according to claim 2, wherein: mixing device (4) still includes direct current motor (43) and hold-in range (44), and direct current motor (43) set up on mixing board (41), and hold-in range (44) are located mixing board (41) below, and direct current motor (43) and mixing axle (422) are connected respectively to hold-in range (44) both ends.

4. A magnetic bead separating and washing apparatus with a mixing structure according to claim 3, wherein: the blending assembly (42) further comprises a synchronizing wheel (426), the synchronizing wheel (426) is sleeved outside the blending shaft (422) below the blending plate (41), and the synchronizing wheel (426) is connected with a synchronous belt (44).

5. The magnetic bead separation and washing apparatus with a mixing structure according to claim 1, wherein: the mounting component comprises a mounting plate (11) and a lifting base (12) arranged on the mounting plate (11), the cleaning disc component is arranged on the mounting plate (11), and the blending device (4) and the needle assembly are arranged on the lifting base (12) in a lifting mode.

6. The magnetic bead separation and washing apparatus with mixing structure of claim 5, wherein: the blending device (4) and the needle assembly are respectively connected with a screw motor.

7. The magnetic bead separation and washing apparatus with mixing structure of claim 5, wherein: the cleaning disc component also comprises a cleaning disc mounting frame fixed on the mounting plate (11), and the cleaning disc (21) is rotatably arranged on the cleaning disc mounting frame.

8. The magnetic bead separation and washing apparatus with a mixing structure according to claim 7, wherein: the cleaning disc component also comprises a stepping motor (23), and the stepping motor (23) is connected with and drives the cleaning disc (21) to rotate in a stepping mode.

9. The magnetic bead separation and washing apparatus with a mixing structure according to claim 7, wherein: the magnetic bead adsorption device (3) comprises a plurality of magnet mounting frames (31) and a plurality of magnets, the magnet mounting frames (31) are fixed on the cleaning disc mounting frame at intervals, and a plurality of magnets are arranged on each cleaning disc mounting frame at intervals.

10. The magnetic bead separation and washing apparatus with a mixing structure according to claim 1, wherein: the needles include a liquid suction needle for sucking waste liquid, a cleaning needle for injecting a cleaning liquid, and a substrate needle for injecting a substrate.

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