CN210765324U - Separate injection cleaning gas-liquid path unit for DNA synthesizer and gas-liquid path system - Google Patents
Separate injection cleaning gas-liquid path unit for DNA synthesizer and gas-liquid path system Download PDFInfo
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- CN210765324U CN210765324U CN201921366641.3U CN201921366641U CN210765324U CN 210765324 U CN210765324 U CN 210765324U CN 201921366641 U CN201921366641 U CN 201921366641U CN 210765324 U CN210765324 U CN 210765324U
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Abstract
The utility model relates to a divide injection washing gas-liquid way unit, gas-liquid way system for DNA synthesizer belongs to the biotechnology field. The gas-liquid path system comprises a 1 st gas branch, a 2 nd gas branch, a 3 rd gas branch, a 5 th gas branch and a separate injection cleaning gas-liquid path unit, a 1 st solenoid valve is arranged on the 1 st gas branch, a 2 nd solenoid valve group is arranged on the 2 nd gas branch, a gas source passes through the 1 st gas branch, the 2 nd gas branch is communicated with a shell and a pressing seal blowing and liquid draining device, the gas source passes through the 3 rd gas branch and the cleaning solenoid valve group, a 5 th solenoid valve group and a 5 th speed regulating valve group are arranged on the 5 th gas branch, and the gas source passes through the 5 th gas branch and the shell. The problems that a DNA synthesizer gas-liquid path system is complex and inconvenient to overhaul are solved; a large amount of gas is wasted due to the reaction conditions of the inert gas environment; the problem that the gas-liquid path system cannot be effectively cleaned.
Description
Technical Field
The utility model relates to the field of biotechnology, especially, relate to a divide and annotate washing gas-liquid way unit, gas-liquid way system for DNA synthesizer.
Background
China scientists have completed the total synthesis of yeast alanine tRNA in 1981, which is the first artificially synthesized RNA molecule with all biological activities in the world, and artificially synthesized DNA is also the only way for known directional modification of gene sequences, and is widely applied to multiple fields of protein modification, life science and the like, such as nucleic acid medicine, enzyme engineering, gene detection, gene therapy and the like. The DNA synthesizer needs deprotection, activation linking, capping, oxidation and other steps in the synthesis process, different reagents need to be injected into the synthesis column under the protection of inert gas in the synthesis process, and the reagents in the synthesis column are emptied after the reaction is completed. The existing DNA synthesizer gas-liquid path system has the following problems: 1. the system is complex and inflexible to operate, users cannot make proper adjustment according to the requirements of the users, and the system is inconvenient to overhaul; 2. a large amount of gas is wasted due to the reaction conditions of the inert gas environment; 3. the gas-liquid path system can not be effectively cleaned, and the problem of steel needle blockage is fundamentally solved. Therefore, a good gas-liquid path system is required in the synthesis process to complete the complicated operations of injecting various reagents, cleaning the cleaning solution, cleaning the inert gas, discharging the waste liquid, and the like.
SUMMERY OF THE UTILITY MODEL
The utility model aims to solve the technical problem that a separate injection washing gas-liquid way unit, gas-liquid way system for DNA synthesizer is provided, the solution system is complicated, can not effectually wash gas-liquid way system, solves the steel needle jam problem from the root.
The utility model provides an above-mentioned technical problem's technical scheme as follows: the utility model provides a divide and annotate washing gas-liquid way unit for DNA synthesizer, includes 4-1 hydraulic branch road 1, 4-2 hydraulic branch road 2 and washing solenoid valve group 3, 4-1 hydraulic branch road 1 is gone up and is set gradually 4-1 ball valve 5 and a plurality of first reagent bottle 4, 4-2 hydraulic branch road 2 is gone up and is set gradually 4-2 ball valve 6 and washing bottle 7, 4-1 hydraulic branch road 1 with 2 parallelly connected modes of 4-2 hydraulic branch road are connected with air supply 8, 4-1 hydraulic branch road 1 with the end of 4-2 hydraulic branch road 2 all with washing solenoid valve group 3 is connected, washing solenoid valve group 3 is connected with dividing the unit 9.
Furthermore, a plurality of second reagent bottles 10 are further arranged on the 4 th-2 th hydraulic branch 2, and the plurality of second reagent bottles 10 and the cleaning bottles 7 are communicated and arranged on the 4 th-2 th hydraulic branch 2.
Further, a first air inlet and a first liquid outlet are formed in the first reagent bottle 4, a cleaning bottle air inlet and a cleaning bottle liquid outlet are formed in the cleaning bottle 7, a second air inlet and a second liquid outlet are formed in the second reagent bottle 10, the first air inlet is communicated with an air source 8 through a 4-1 ball valve 5, the cleaning bottle air inlet and the second air inlet are communicated with the air source 8 through a 4-2 ball valve 6, the first liquid outlet, the cleaning bottle liquid outlet and the second liquid outlet are connected with the cleaning electromagnetic valve group 3, and the cleaning bottle liquid outlet is further connected with a separate injection unit 9.
Further, the device also comprises a 4 th-3 rd gas branch 11, wherein the 4 th-3 rd gas branch 11 is connected with the 4 th-2 nd ball valve 6 in a manner of being parallel to the 4 th-2 nd hydraulic branch 2, and the tail end of the 4 th-3 rd gas branch 11 is connected with a waste liquid and waste gas system.
The utility model provides a gas-liquid way system for DNA synthesizer, includes 1 st gas branch road 12, 2 nd gas branch road 14, 3 rd gas branch road 20, 5 th gas branch road 23 and above-mentioned branch injection washing gas-liquid way unit, be provided with 1 st solenoid valve 13 on 1 st gas branch road 12, be provided with 2 nd solenoid valve group on the 2 nd gas branch road 14, the air supply 8 passes through 1 st gas branch road 12 2 nd gas branch road 14 is linked together with casing 22 and the sealed drain 21 that blows that compresses tightly, the air supply 8 passes through 3 rd gas branch road 20 with washing solenoid valve group 3 is linked together, be provided with 5 th solenoid valve group and 5 speed governing valves on the 5 th gas branch road 23, the air supply 8 passes through 5 th gas branch road 23 is linked together with casing 22.
Further, the 2 nd gas branch 14 is also provided with a 1 st speed regulating valve 19.
Further, the 2 nd gas branch 14 includes a 2 nd-1 st gas branch 15 and a 2 nd-2 nd gas branch 16, the 2 nd-1 st gas branch 15 and the 2 nd-2 nd gas branch 16 are connected in parallel to communicate with the gas source 8, the 2 nd solenoid valve group includes a 2 nd-1 st solenoid valve 17 and a 2 nd-2 nd solenoid valve 18, the 2 nd-1 st solenoid valve 17 is disposed on the 2 nd-1 st gas branch 15, and the 2 nd-2 nd solenoid valve 18 and the 1 st speed regulating valve 19 are disposed on the 2 nd-2 nd gas branch 16.
Further, the 5 th electromagnetic valve group comprises a 5 th-1 electromagnetic valve 24 and a 5 th-2 electromagnetic valve 25, the 5 th speed regulating valve group comprises a 5 th-1 speed regulating valve 26 and a 5 th-2 speed regulating valve 27, and the air source 8 is communicated with the shell 22 through the 5 th-1 electromagnetic valve 24, the 5 th-1 speed regulating valve 26, the 5 th-2 electromagnetic valve 25 and the 5 th-2 speed regulating valve 27 in sequence.
Further, a pressure regulating valve 28 and a pressure gauge 29 are arranged on the 1 st gas branch 12 and the 2 nd gas branch 14, and a one-way pressure regulating valve 30 and a pressure gauge 29 are arranged on the 4 th-1 st hydraulic branch 1 and the 4 th-2 nd hydraulic branch 2.
The utility model provides a divide and annotate washing gas-liquid way unit for DNA synthesizer, including 4-1 hydraulic branch road 1, 4-2 hydraulic branch road 2 and washing electromagnetism valves 3, 4-1 hydraulic branch road 1 is gone up and has set gradually 4-1 ball valve 5 and a plurality of first reagent bottle 4, 4-2 ball valve 6 and washing bottle 7 have set gradually on 4-2 hydraulic branch road 2, 4-1 hydraulic branch road 1 with 4-2 hydraulic branch road 2 parallel mode is connected with air supply 8, 4-1 hydraulic branch road 1 with 4-2 hydraulic branch road 2's end all with washing electromagnetism valves 3 is connected, washing electromagnetism valves 3 is connected with dividing the unit 9. Thus, different reagents are respectively filled in the first reagent bottles 4, the 4-1 ball valve 5 can be controlled to control gas to pass through the 4-1 hydraulic branch 1 and then to apply gas pressure to the first reagent bottles 4 so as to enable the reagents in the first reagent bottles 4 to be hydraulically pressed into the 4-1 hydraulic branch 1, and then the reagents respectively enter the cleaning electromagnetic valve group 3, and the cleaning electromagnetic valve group 3 is respectively communicated with the dispensing unit 9, so that the 4-1 hydraulic branch 1 can be used by being matched with the cleaning electromagnetic valve group 3 and the dispensing unit 9, and injection of various different reagents can be realized; the cleaning bottle 7 is filled with cleaning liquid, the 4 th-2 ball valve 6 can be controlled to control the cleaning reagent in the cleaning bottle 7 to be hydraulically pressed into the 4 th-2 hydraulic branch 2 by applying gas pressure to the cleaning bottle 7 after gas passes through the 4 th-2 hydraulic branch 2, then respectively enters a cleaning electromagnetic valve group 3 and a dispensing unit 9, the cleaning electromagnetic valve group 3 is respectively communicated with the dispensing unit 9, therefore, the 4 th-2 th hydraulic branch 2 can realize the cleaning of the cleaning liquid to each pipeline and the dispensing unit 9 by matching with the cleaning electromagnetic valve group 3 and the dispensing unit 9, and the 4 th-1 hydraulic branch 1 and the 4 th-2 hydraulic branch 2 are respectively provided with a 4 th-1 ball valve 5 and a 4 th-2 ball valve 6, so that the maintenance and the overhaul are convenient, and a user can conveniently make adjustment according to the requirement.
The utility model provides a gas-liquid way system for DNA synthesizer, including the 1 st gas branch road 12, the 2 nd gas branch road 14, the 3 rd gas branch road 20, the 5 th gas branch road 23 and the aforesaid divide and annotate the injection and wash gas-liquid way unit, be provided with the 1 st solenoid valve 13 on the 1 st gas branch road 12, be provided with the 2 nd electromagnetism valves on the 2 nd gas branch road 14, the air supply 8 passes through the 1 st gas branch road 12 the 2 nd gas branch road 14 is linked together with casing 22 and the sealed drain 21 that blows that compresses tightly, and the air supply 8 passes through the 3 rd gas branch road 20 with wash electromagnetism valves 3 and be linked together, be provided with the 5 th electromagnetism valves and the 5 th speed governing valves on the 5 th gas branch road 23, the air supply 8 passes through the 5 th gas branch road 23 is linked together with casing 22. Thus, for the 1 st gas branch 12, when the 1 st electromagnetic valve 13 is powered on, the gas source 8 is communicated with the pressing and sealing gas blowing and liquid discharging device 21 through the 1 st gas branch 12, at this time, the pressing and sealing gas blowing and liquid discharging device 21 is mainly used for inflating a pressing and sealing synthetic plate, the synthetic plate is sealed, when the 1 st electromagnetic valve 13 is powered off, the gas source 8 is communicated with the shell 22 through the 1 st gas branch 12, at this time, the shell 22 is filled with inert gas mainly for providing inert protective gas for the whole shell 22, meanwhile, the gas in the pressing and sealing gas blowing and liquid discharging device 21 can also enter the shell 22 through the 1 st electromagnetic valve 13, and the gas in the pressing and sealing gas blowing and liquid discharging device 21 is recycled; for the 2 nd gas branch 14, when the 2 nd electromagnetic valve is powered on, the gas source 8 is communicated with the pressing sealing gas blowing and liquid discharging device 21 through the 2 nd gas branch 14, at the moment, the gas is filled into the pressing sealing gas blowing and liquid discharging device 21 mainly for accurately controlling the reaction and liquid discharging conditions of the reagents in the synthesis column, when the 2 nd electromagnetic valve is powered off, the gas source 8 is communicated with the shell 22 through the 2 nd gas branch 14, at the moment, the inert gas is filled into the shell 22 mainly for providing inert protective gas for the whole shell 22, meanwhile, the gas in the pressing sealing gas blowing and liquid discharging device 21 can also enter the shell 22 through the 2 nd electromagnetic valve, and the gas in the pressing sealing gas blowing and liquid discharging device 21 is recycled; 3 gas branch 20 is linked together with washing solenoid valve group 3, and washing solenoid valve group 3 is connected with dividing the injection unit 9 again, and air supply 8 provides inert purge gas for washing solenoid valve group 3 through 3 gas branch 20 like this, can realize aerifing for casing 22 through 5 gas branch 23 to each pipeline and the washing of dividing injection unit 9, and the flow of gas can be adjusted to 5 speed governing valves.
Drawings
FIG. 1 is a schematic diagram of a separate injection cleaning gas-liquid path unit and a gas-liquid path system for a DNA synthesizer according to the present invention;
in the drawings, the components represented by the respective reference numerals are listed below:
1. a 4-1 hydraulic branch, a 2, a 4-2 hydraulic branch, a 3, a cleaning electromagnetic valve group, a 4, a first reagent bottle, a 5, a 4-1 ball valve, a 6, a 4-2 ball valve, a 7, a cleaning bottle, an 8, a gas source, a 9, a dispensing unit, a 10, a second reagent bottle, a 11, a 4-3 gas branch, a 12, a 1 gas branch, a 13, a 1 electromagnetic valve, a 14, a 2 gas branch, a 15, a 2-1 gas branch, a 16, a 2-2 gas branch, a 17, a 2-1 electromagnetic valve, a 18, a 2-2 electromagnetic valve, a 19, a 1 speed regulating valve, a 20, a 3 gas branch, a 21, a compression sealing air blowing and draining device, a 22, a shell, a 23, a 5 gas branch, a 24, a 5-1 electromagnetic valve, a 25, a 5-2 electromagnetic valve, a 26, a 5-1 speed regulating valve, 27. the device comprises a No. 5-2 speed regulating valve, a 28, a pressure regulating valve, a 29, a pressure gauge, a 30, a one-way pressure regulating valve, a 31, a first through hole, a 32, a second through hole, a 33, a movable tray, a 34, an air pump, a 35, a three-way ball valve, a 36, a first two-way ball valve, a 37, a second two-way ball valve, a 38, a waste liquid barrel, a 39, a liquid inlet, a 40, an air pumping port, a 41 and a liquid discharging port.
Detailed Description
The principles and features of the present invention are described below in conjunction with the following drawings, the examples given are only intended to illustrate the present invention and are not intended to limit the scope of the present invention.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "center", "inner", "outer", "top", "bottom", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention.
In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.
As shown in fig. 1, the utility model provides a divide injection washing gas-liquid way unit for DNA synthesizer, including 4-1 hydraulic branch road 1, 4-2 hydraulic branch road 2 and washing solenoid valve group 3, 4-1 hydraulic branch road 1 is gone up and has been set gradually 4-1 ball valve 5 and a plurality of first reagent bottle 4, 4-2 ball valve 6 and washing bottle 7 have been set gradually on 4-2 hydraulic branch road 2, 4-1 hydraulic branch road 1 with 4-2 hydraulic branch road 2 parallel mode is connected with air supply 8, 4-1 hydraulic branch road 1 with the end of 4-2 hydraulic branch road 2 all with washing solenoid valve group 3 is connected, wash solenoid valve group 3 and divide injection unit 9 to be connected. Thus, different reagents are respectively filled in the first reagent bottles 4, the 4-1 ball valve 5 can be controlled to control gas to pass through the 4-1 hydraulic branch 1 and then to apply gas pressure to the first reagent bottles 4 so as to enable the reagents in the first reagent bottles 4 to be hydraulically pressed into the 4-1 hydraulic branch 1, and then the reagents respectively enter the cleaning electromagnetic valve group 3, and the cleaning electromagnetic valve group 3 is respectively communicated with the separate injection unit 9, so that the 4-1 hydraulic branch 1 is matched with the cleaning electromagnetic valve group 3 and the separate injection unit 9 for use, and injection of various different reagents can be realized; the cleaning bottle 7 is filled with cleaning liquid, the 4 th-2 ball valve 6 can be controlled to control the cleaning reagent in the cleaning bottle 7 to be hydraulically pressed into the 4 th-2 hydraulic branch 2 by applying gas pressure to the cleaning bottle 7 after gas passes through the 4 th-2 hydraulic branch 2, then respectively enters a cleaning electromagnetic valve group 3 and a dispensing unit 9, the cleaning electromagnetic valve group 3 is respectively communicated with the dispensing unit 9, therefore, the 4 th-2 th hydraulic branch 2 can realize the cleaning of the cleaning liquid to each pipeline and the dispensing unit 9 by matching with the cleaning electromagnetic valve group 3 and the dispensing unit 9, and the 4 th-1 hydraulic branch 1 and the 4 th-2 hydraulic branch 2 are respectively provided with a 4 th-1 ball valve 5 and a 4 th-2 ball valve 6, so that the maintenance and the overhaul are convenient, and a user can conveniently make adjustment according to the requirement.
The cleaning electromagnetic valve group 3 comprises an electromagnetic valve seat, a cleaning electromagnetic valve and a plurality of first electromagnetic valves, wherein a main channel, a cleaning liquid inlet, a gas inlet, a plurality of medicament inlets and a plurality of liquid outlets are formed in the electromagnetic valve seat, the positions of the plurality of first electromagnetic valves, the plurality of medicament inlets and the plurality of liquid outlets are in one-to-one correspondence, the medicament inlets and the liquid outlets are connected with the main channel through the corresponding first electromagnetic valves, and the cleaning liquid inlets and the gas inlet are connected with the main channel through the cleaning electromagnetic valve. Therefore, when the reagent dispensing device is normally used, after a certain reagent enters from the reagent liquid inlet, the corresponding first electromagnetic valves are switched through the valves, so that the reagent directly flows out through the corresponding liquid outlets and then respectively enters the corresponding pipelines of the dispensing unit 9; when the pipeline and the syringe needle that a certain reagent corresponds are washd with the washing liquid in the washing bottle 7 as required, the washing liquid gets into the back from the washing liquid inlet, washs the solenoid valve and passes through the valve switching for the washing liquid directly gets into the main entrance, at this moment, the first solenoid valve that this reagent corresponds passes through the valve switching, makes the liquid outlet that this reagent corresponds be linked together with the main entrance, the washing liquid gets into the liquid outlet that this reagent corresponds behind the main entrance through the flow of main entrance, and then the washing of the corresponding pipeline and the syringe needle of this reagent branch injection device is realized.
The separate injection unit 9 comprises a plurality of capillary tube connecting structures, a plurality of separate injection electromagnetic valve sets and a plurality of separate injection needle fixing structures, each separate injection electromagnetic valve set comprises an electromagnetic valve fixing seat, a plurality of second electromagnetic valve seats and a plurality of second electromagnetic valves, a liquid inlet is formed in one end of each second electromagnetic valve seat, a plurality of liquid outlets are formed in the side face of each electromagnetic valve seat, one ends of a plurality of capillary tubes are respectively connected with the liquid outlets in the side face of each electromagnetic valve seat, and the other ends of the plurality of capillary tubes are respectively connected with the synthesis columns on the synthesis plate through the separate injection needle fixing structures. Thus, when in normal use, the reagents flowing out from the plurality of liquid outlets of the cleaning electromagnetic valve group 3 respectively enter the liquid inlets of the plurality of dispensing electromagnetic valve groups in the dispensing unit 9, and the reagents respectively enter the synthesis columns on the synthesis plate through the plurality of capillary tubes.
The utility model discloses a divide injection washing gas-liquid way unit for DNA synthesizer, as shown in FIG. 1, can also be on the basis of the technical scheme of preceding description: the 4 th-2 th hydraulic branch 2 is also provided with a plurality of second reagent bottles 10, and the plurality of second reagent bottles 10 and the cleaning bottle 7 are communicated and arranged on the 4 th-2 th hydraulic branch 2. Like this, different reagents are respectively contained in a plurality of second reagent bottles 10, can control gas through 4 th-2 hydraulic branch 2 after through exerting gas pressure in a plurality of second reagent bottles 10 and with the reagent hydraulic pressure in a plurality of second reagent bottles 10 to 4 th-2 hydraulic branch 2 through controlling 4 th-2 ball valve 6, then get into respectively and wash solenoid valve group 3 in, wash solenoid valve group 3 and be linked together with dispensing unit 9 respectively again, consequently, 4 th-2 hydraulic branch 2 can realize the injection of multiple different reagents through the cooperation use with washing solenoid valve group 3 and dispensing unit 9.
Further preferred technical scheme is that a plurality of first reagent bottles 4 can be used for depositing different monomer reagents, a plurality of second reagent bottles 10 can be used for depositing different auxiliary reagents, monomer reagent bottles and auxiliary reagent bottles set up respectively on different hydraulic pressure branches, can conveniently overhaul.
The further preferred technical scheme is that two or more reagent bottles can be selected to store the same reagent, and the liquid outlet of one reagent bottle is communicated with the air inlet of the other reagent bottle, so that the reagent storage amount can be increased, the reagent bottle replacement frequency is reduced, and the reagent replacement caused by interrupting a synthesis program in the synthesis process is avoided.
The utility model discloses a divide injection washing gas-liquid way unit for DNA synthesizer, as shown in FIG. 1, can also be on the basis of the technical scheme of preceding description: the reagent bottle cleaning device is characterized in that a first air inlet and a first liquid outlet are formed in the first reagent bottle 4, a cleaning bottle air inlet and a cleaning bottle liquid outlet are formed in the cleaning bottle 7, a second air inlet and a second liquid outlet are formed in the second reagent bottle 10, the first air inlet is communicated with an air source 8 through a 4-1 ball valve 5, the cleaning bottle air inlet is communicated with the second air inlet through a 4-2 ball valve 6 and the air source 8, the first liquid outlet, the cleaning bottle liquid outlet and the second liquid outlet are connected with the cleaning electromagnetic valve group 3, and the cleaning bottle liquid outlet is further connected with a separate injection unit 9. Thus, the air source 8 is communicated with the first air inlet through a 4 th-1 st electromagnet, the reagent hydraulic pressure in the first reagent bottle 4 flows out to the corresponding reagent inlet of the cleaning electromagnetic valve group 3 through the first liquid outlet, the air source 8 is communicated with the second air inlet and the cleaning bottle air inlet through a 4 th-2 th ball valve 6, the reagent in the second reagent bottle 10 and the cleaning liquid hydraulic pressure in the cleaning bottle 7 respectively flow out to the corresponding reagent inlet and the cleaning liquid inlet of the cleaning electromagnetic valve group 3 through the corresponding second liquid outlet and the cleaning bottle liquid outlet, so as to provide corresponding reagent for separate injection and cleaning, the cleaning bottle liquid outlet is also connected with a separate injection unit 9, and the cleaning liquid is directly provided for the separate injection unit 9.
The utility model discloses a divide injection washing gas-liquid way unit for DNA synthesizer, as shown in FIG. 1, can also be on the basis of the technical scheme of preceding description: the device also comprises a 4 th-3 rd gas branch 11, wherein the 4 th-3 rd gas branch 11 is connected with the 4 th-2 nd ball valve 6 in a manner of being parallel to the 4 th-2 nd hydraulic branch 2, and the tail end of the 4 th-3 rd gas branch 11 is connected with a waste liquid and waste gas system. Thus, the 4 th-3 rd gas branch 11 is arranged behind the 4 th-2 nd ball valve 6 and is connected with the waste liquid and waste gas system, and the 4 th-3 rd gas branch 11 can be controlled to provide the gas source 8 for the waste liquid and waste gas system by controlling the 4 th-2 nd ball valve 6.
The waste liquid and waste gas system comprises an air pump 34, a waste liquid barrel 38, a three-way ball valve 35, a first two-way ball valve 36 and a second two-way ball valve 37, wherein an air suction port 40, a liquid inlet 39 and a liquid outlet 41 are arranged on the waste liquid barrel 38, the first two-way ball valve 36 is arranged on a pipeline corresponding to the liquid outlet 41 on the waste liquid barrel 38, the liquid inlet 39 on the waste liquid barrel 38 is connected with a movable tray 33 in the shell 22 through the second two-way ball valve 37, and three ports of the three-way ball valve 35 are respectively communicated with the air pump 34, the 4 th-3 rd gas branch 11 and the air suction port 40 of the waste liquid barrel 38. Thus, during normal operation, the air pump 34 is communicated with the pumping hole 40 of the waste liquid barrel 38, the air pump 34 pumps air to enable the waste liquid barrel 38 to generate a certain negative pressure, so as to suck the waste liquid in the movable tray 33 and the waste gas in the shell 22, and enable the waste liquid to be discharged into the waste liquid barrel 38, the waste gas is discharged to the exhaust hole through the air pump 34, when the waste liquid barrel 38 is fully loaded, the 4 th-3 th gas branch 11 is communicated with the pumping hole 40 of the waste liquid barrel 38, the second two-way ball valve 37 is closed, the first two-way ball valve 36 is opened, and the gas source 8 charges air into the waste liquid barrel 38 through the 4 th-3 th gas branch 11 to generate a positive pressure to discharge the waste.
The synthetic board is placed on the movable tray 33, after the reaction is completed, the waste liquid in the synthetic column on the synthetic board is discharged into the movable tray 33, the shell 22 mainly provides inert protection environment for the reaction, therefore, when the air pump 34 pumps air to make the waste liquid barrel 38 generate certain negative pressure, the waste liquid in the movable tray 33 and the waste gas in the shell 22 can be sucked.
The utility model provides a gas-liquid way system for DNA synthesizer, including the 1 st gas branch road 12, the 2 nd gas branch road 14, the 3 rd gas branch road 20, the 5 th gas branch road 23 and the aforesaid divide and annotate the injection and wash gas-liquid way unit, be provided with the 1 st solenoid valve 13 on the 1 st gas branch road 12, be provided with the 2 nd electromagnetism valves on the 2 nd gas branch road 14, the air supply 8 passes through the 1 st gas branch road 12 the 2 nd gas branch road 14 is linked together with casing 22 and the sealed drain 21 that blows that compresses tightly, and the air supply 8 passes through the 3 rd gas branch road 20 with wash electromagnetism valves 3 and be linked together, be provided with the 5 th electromagnetism valves and the 5 th speed governing valves on the 5 th gas branch road 23, the air supply 8 passes through the 5 th gas branch road 23 is linked together with casing 22. Thus, for the 1 st gas branch 12, when the 1 st electromagnetic valve 13 is powered on, the gas source 8 is communicated with the pressing and sealing gas blowing and liquid discharging device 21 through the 1 st gas branch 12, at this time, the pressing and sealing gas blowing and liquid discharging device 21 is mainly used for inflating a pressing and sealing synthetic plate, the synthetic plate is sealed, when the 1 st electromagnetic valve 13 is powered off, the gas source 8 is communicated with the shell 22 through the 1 st gas branch 12, at this time, the shell 22 is filled with inert gas mainly for providing inert protective gas for the whole shell 22, meanwhile, the gas in the pressing and sealing gas blowing and liquid discharging device 21 can also enter the shell 22 through the 1 st electromagnetic valve 13, and the gas in the pressing and sealing gas blowing and liquid discharging device 21 is recycled; for the 2 nd gas branch 14, when the 2 nd electromagnetic valve is powered on, the gas source 8 is communicated with the pressing sealing gas blowing and liquid discharging device 21 through the 2 nd gas branch 14, at the moment, the gas is filled into the pressing sealing gas blowing and liquid discharging device 21 mainly for accurately controlling the reaction and liquid discharging conditions of the reagents in the synthesis column, when the 2 nd electromagnetic valve is powered off, the gas source 8 is communicated with the shell 22 through the 2 nd gas branch 14, at the moment, the inert gas is filled into the shell 22 mainly for providing inert protective gas for the whole shell 22, meanwhile, the gas in the pressing sealing gas blowing and liquid discharging device 21 can also enter the shell 22 through the 2 nd electromagnetic valve, and the gas in the pressing sealing gas blowing and liquid discharging device 21 is recycled; 3 gas branch 20 is linked together with washing solenoid valve group 3, and washing solenoid valve group 3 is connected with dividing the injection unit 9 again, and air supply 8 provides inert purge gas for washing solenoid valve group 3 through 3 gas branch 20 like this, can realize aerifing for casing 22 through 5 gas branch 23 to each pipeline and the washing of dividing injection unit 9, and the flow of gas can be adjusted to 5 speed governing valves.
The compressing, sealing, blowing and draining device 21 comprises a cylinder body and a piston assembly, wherein the piston assembly is arranged inside the cylinder body in a sliding mode, a first cavity is formed between the upper portion of the piston assembly and the inside of the cylinder body, the outer side face of the piston assembly is connected with the inner surface of the cylinder body in a sealing mode, a first through hole 31 and a second through hole 32 are formed in the cylinder body, the first through hole 31 is communicated with the first cavity, a third through hole is formed in the inner side of the piston assembly, and the second through hole 32 is communicated with the third through hole. Thus, when the 1 st gas branch 12 charges the first cavity through the first through hole 31 formed on the cylinder body, because the outer side surface of the piston assembly is connected with the inner surface of the cylinder body in a sealing way, so that the first chamber generates a large gas pressure to force the piston assembly to move downwards and further press the synthesis plate on the DNA synthesizer, so that a second chamber is formed between the piston assembly and the synthesis plate on the DNA synthesizer, the 2 nd gas branch 14 charges the second cavity through a second through hole 32 formed on the cylinder body, the communication between the second through hole 32 and the second cavity is realized through a third through hole which is arranged on the inner side surface of the piston assembly and communicated with the second through hole 32, because the second cavity forms a compression sealing system at this time, the second through hole 32 is inflated to form a certain gas pressure, so that the waste liquid in the synthetic column can be emptied quickly and the reaction degree of each reagent can be controlled.
It should be noted here that, because of being provided with the gas inlet on the washing solenoid valve group 3, 3 rd gas branch 20 is linked together with the gas inlet on the washing solenoid valve group 3, like this, when needs wash pipeline and the syringe needle that a certain reagent corresponds with gas, gaseous back of getting into from the gas inlet on the washing solenoid valve group 3, the washing solenoid valve passes through the valve and switches, make gaseous direct entering main entrance, at this moment, the first solenoid valve that this reagent corresponds passes through the valve and switches, make the liquid outlet that this reagent corresponds be linked together with the main entrance, gaseous flow through get into the liquid outlet that this reagent corresponds behind the main entrance, and then realize the washing to this reagent dispensing device's corresponding pipeline and syringe needle.
The gas-liquid path system for DNA synthesizer of the present invention, as shown in fig. 1, can also be based on the above-described technical solution: the 2 nd gas branch 14 is also provided with a 1 st speed regulating valve 19. Thus, the 1 st speed valve 19 can regulate the gas flow of the gas charge.
The gas-liquid path system for DNA synthesizer of the present invention, as shown in fig. 1, can also be based on the above-described technical solution: the 2 nd gas branch 14 comprises a 2 nd-1 st gas branch 15 and a 2 nd-2 nd gas branch 16, the 2 nd-1 st gas branch 15 and the 2 nd-2 nd gas branch 16 are communicated with the gas source 8 in a parallel mode, the 2 nd electromagnetic valve group comprises a 2 nd-1 st electromagnetic valve 17 and a 2 nd-2 nd electromagnetic valve 18, the 2 nd-1 st electromagnetic valve 17 is arranged on the 2 nd-1 st gas branch 15, and the 2 nd-2 nd electromagnetic valve 18 and the 1 st speed regulating valve 19 are arranged on the 2 nd-2 nd gas branch 16. Thus, the 2 nd-1 st gas branch 15 and the 2 nd-2 nd gas branch 16 are communicated with the gas source 8 in a parallel mode, the 2 nd-1 st gas branch 15 is provided with a 2 nd-1 st electromagnetic valve 17, the 2 nd-2 nd gas branch 16 is provided with a 2 nd-2 nd electromagnetic valve 18 and a 1 st speed regulating valve 19, for the 2 nd-1 st gas branch 15, when the 2 nd-1 st electromagnetic valve 17 is powered on, the gas source 8 provides gas for the pressing sealing gas blowing and liquid discharging device 21 through the 2 nd-1 st gas branch 15, when the 2 nd-1 st electromagnetic valve 17 is powered off, the gas in the pressing sealing gas blowing and liquid discharging device 21 can enter the shell 22 through the 2 nd-1 st electromagnetic valve 17, so as to realize effective utilization of waste gas, for the 2 nd-2 nd gas branch 16, when the 2 nd-2 nd electromagnetic valve 18 is powered on, the gas source 8 blows gas for the pressing sealing gas blowing and, the 1 st speed regulating valve 19 can regulate the gas flow to press the sealed gas blowing and liquid discharging device 21 to blow small gas, and when the 2 nd-2 nd electromagnetic valve 18 is powered off, the gas filling is stopped.
The gas-liquid path system for DNA synthesizer of the present invention, as shown in fig. 1, can also be based on the above-described technical solution: the 5 th electromagnetic valve group comprises a 5 th-1 electromagnetic valve 24 and a 5 th-2 electromagnetic valve 25, the 5 th speed regulating valve group comprises a 5 th-1 speed regulating valve 26 and a 5 th-2 speed regulating valve 27, and an air source 8 sequentially passes through the 5 th-1 electromagnetic valve 24, the 5 th-1 speed regulating valve 26, the 5 th-2 electromagnetic valve 25 and the 5 th-2 speed regulating valve 27 and is communicated with the shell 22. Therefore, the 5-1 speed regulating valve 26 regulates the gas flow to be smaller, the 5-2 speed regulating valve 27 regulates the gas flow to be larger, when the 5-1 electromagnetic valve 24 and the 5-2 electromagnetic valve 25 are both powered on, the gas source 8 sequentially passes through the 5-1 electromagnetic valve 24, the 5-1 speed regulating valve 26 and the 5-2 electromagnetic valve 25 to fill air into the shell 22, when the 5-1 electromagnetic valve 24 is powered on and the 5-2 electromagnetic valve 25 is powered off, the gas source 8 sequentially passes through the 5-1 electromagnetic valve 24, the 5-1 speed regulating valve 26, the 5-2 electromagnetic valve 25 and the 5-2 speed regulating valve 27 to fill air into the shell 22 to be smaller, and when the 5-1 electromagnetic valve 24 is powered off, the air filling is stopped, the whole system is flexible, and a user can conveniently adjust the gas flow according to own needs.
The gas-liquid path system for DNA synthesizer of the present invention, as shown in fig. 1, can also be based on the above-described technical solution: the 1 st gas branch 12 and the 2 nd gas branch 14 are provided with a pressure regulating valve 28 and a pressure gauge 29, and the 4 th-1 st hydraulic branch 1 and the 4 th-2 nd hydraulic branch 2 are provided with a one-way pressure regulating valve 30 and a pressure gauge 29. Thus, the pressure regulating valve 28 is arranged to facilitate users to make adjustments as required in time, and the pressure gauge 29 is arranged to facilitate users to observe the pressure value of the system in real time.
It should be noted here that the one-way pressure regulating valve 30 and the pressure gauge 29 provided in the 4 th-1 st hydraulic branch 1 and the 4 th-2 nd hydraulic branch 2 may be provided in a main path common to the 4 th-1 st hydraulic branch 1 and the 4 th-2 nd hydraulic branch 2.
The above description is only for the preferred embodiment of the present invention, and is not intended to limit the present invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included within the protection scope of the present invention.
Claims (9)
1. A separate injection cleaning gas-liquid path unit for a DNA synthesizer is characterized in that: the separate injection cleaning gas-liquid path unit comprises a 4-1 hydraulic branch (1), a 4-2 hydraulic branch (2) and a cleaning electromagnetic valve group (3), a 4-1 ball valve (5) and a plurality of first reagent bottles (4) are sequentially arranged on the 4-1 hydraulic branch (1), a 4-2 ball valve (6) and a cleaning bottle (7) are sequentially arranged on the 4-2 hydraulic branch (2), the 4-1 hydraulic branch (1) and the 4-2 hydraulic branch (2) are connected with a gas source (8) in a parallel mode, the 4-1 hydraulic branch (1) and the 4-2 hydraulic branch (2) are connected with the cleaning electromagnetic valve group (3) at the tail ends, and the cleaning electromagnetic valve group (3) is connected with the separate injection unit (9).
2. The dispensing cleaning gas-liquid path unit according to claim 1, characterized in that: a plurality of second reagent bottles (10) are further arranged on the 4 th-2 hydraulic branch (2), and the second reagent bottles (10) and the cleaning bottle (7) are communicated and arranged on the 4 th-2 hydraulic branch (2).
3. The dispensing cleaning gas-liquid path unit according to claim 2, characterized in that: all seted up first air inlet and first liquid outlet on first reagent bottle (4), set up wash bottle air inlet and wash bottle liquid outlet on wash bottle (7), all seted up second air inlet and second liquid outlet on second reagent bottle (10), first air inlet is linked together with air supply (8) through 4-1 ball valve (5), wash bottle air inlet with the second air inlet is linked together with air supply (8) through 4-2 ball valve (6), first liquid outlet wash bottle liquid outlet with the second liquid outlet all with wash electromagnetism valves (3) and be connected, wash bottle liquid outlet still with divide the injection unit (9) and be connected.
4. The dispensing cleaning gas-liquid path unit according to any one of claims 1 to 3, characterized in that: the device also comprises a 4-3 gas branch (11), wherein the 4-3 gas branch (11) is connected with the 4-2 ball valve (6) in a manner of being parallel to the 4-2 hydraulic branch (2), and the tail end of the 4-3 gas branch (11) is connected with a waste liquid and waste gas system.
5. A gas-liquid path system for a DNA synthesizer, characterized in that: comprising a 1 st gas branch (12), a 2 nd gas branch (14), a 3 rd gas branch (20), a 5 th gas branch (23) and the dispensing cleaning gas-liquid path unit of any one of claims 1 to 4, a 1 st electromagnetic valve (13) is arranged on the 1 st gas branch (12), a 2 nd electromagnetic valve group is arranged on the 2 nd gas branch (14), a gas source (8) is communicated with a shell (22) and a pressing, sealing, blowing and liquid discharging device (21) through the 1 st gas branch (12) and the 2 nd gas branch (14), the gas source (8) is communicated with the cleaning electromagnetic valve group (3) through the 3 rd gas branch (20), and a 5 th electromagnetic valve group and a 5 th speed regulating valve group are arranged on the 5 th gas branch (23), and a gas source (8) is communicated with the shell (22) through the 5 th gas branch (23).
6. The gas-liquid path system for a DNA synthesizer according to claim 5, wherein: the 2 nd gas branch (14) is also provided with a 1 st speed regulating valve (19).
7. The gas-liquid path system for a DNA synthesizer according to claim 6, wherein: the 2 nd gas branch (14) comprises a 2 nd-1 st gas branch (15) and a 2 nd-2 nd gas branch (16), the 2 nd-1 st gas branch (15) and the 2 nd-2 nd gas branch (16) are communicated with a gas source (8) in a parallel mode, the 2 nd electromagnetic valve group comprises a 2 nd-1 st electromagnetic valve (17) and a 2 nd-2 nd electromagnetic valve (18), the 2 nd-1 st electromagnetic valve (17) is arranged on the 2 nd-1 st gas branch (15), and the 2 nd-2 nd electromagnetic valve (18) and the 1 st speed regulating valve (19) are arranged on the 2 nd-2 nd gas branch (16).
8. The gas-liquid path system for a DNA synthesizer according to claim 5, wherein: the 5 th electromagnetic valve group comprises a 5-1 th electromagnetic valve (24) and a 5-2 th electromagnetic valve (25), the 5 th speed regulating valve group comprises a 5-1 th speed regulating valve (26) and a 5-2 th speed regulating valve (27), and an air source (8) sequentially passes through the 5-1 th electromagnetic valve (24), the 5-1 th speed regulating valve (26), the 5-2 th electromagnetic valve (25) and the 5-2 th speed regulating valve (27) and is communicated with the shell (22).
9. A gas-liquid path system for a DNA synthesizer according to any one of claims 5 to 8, wherein: the 1 st gas branch (12) and the 2 nd gas branch (14) are provided with a pressure regulating valve (28) and a pressure gauge (29), and the 4 th-1 st hydraulic branch (1) and the 4 th-2 nd hydraulic branch (2) are provided with a one-way pressure regulating valve (30) and a pressure gauge (29).
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| Application Number | Priority Date | Filing Date | Title |
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| CN201921366641.3U CN210765324U (en) | 2019-08-22 | 2019-08-22 | Separate injection cleaning gas-liquid path unit for DNA synthesizer and gas-liquid path system |
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| CN201921366641.3U CN210765324U (en) | 2019-08-22 | 2019-08-22 | Separate injection cleaning gas-liquid path unit for DNA synthesizer and gas-liquid path system |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110317726A (en) * | 2019-08-22 | 2019-10-11 | 北京擎科生物科技有限公司 | A kind of dispensing purgative gas fluid path unit, gas liquid channel system for DNA synthesizer |
| CN111943999A (en) * | 2020-07-31 | 2020-11-17 | 北京擎科生物科技有限公司 | DNA synthesizer |
| CN113528333A (en) * | 2021-07-20 | 2021-10-22 | 北京擎科生物科技有限公司 | Nucleic acid synthesis reaction apparatus and nucleic acid synthesis method |
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2019
- 2019-08-22 CN CN201921366641.3U patent/CN210765324U/en active Active
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110317726A (en) * | 2019-08-22 | 2019-10-11 | 北京擎科生物科技有限公司 | A kind of dispensing purgative gas fluid path unit, gas liquid channel system for DNA synthesizer |
| CN110317726B (en) * | 2019-08-22 | 2023-09-22 | 北京擎科生物科技股份有限公司 | A divide and annotate washing gas-liquid way unit, gas-liquid way system for DNA synthesizer |
| CN111943999A (en) * | 2020-07-31 | 2020-11-17 | 北京擎科生物科技有限公司 | DNA synthesizer |
| CN111943999B (en) * | 2020-07-31 | 2021-12-17 | 北京擎科生物科技有限公司 | DNA synthesizer |
| CN113528333A (en) * | 2021-07-20 | 2021-10-22 | 北京擎科生物科技有限公司 | Nucleic acid synthesis reaction apparatus and nucleic acid synthesis method |
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Address after: 100176 Floor 6, West Half Unit, Building 3, Yard 105, Jinghai 3rd Road, Daxing District, Beijing Economic-Technological Development Area Patentee after: Beijing Qingke Biotechnology Co.,Ltd. Address before: Building 5, Building 156, Jinghai Industrial Park, Daxing District Economic Development Zone, Beijing, 100176 Patentee before: Beijing Qingke Biotechnology Co.,Ltd. |
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