CN210495318U

CN210495318U - Micromolecular biomass extraction and purification equipment

Info

Publication number: CN210495318U
Application number: CN201920906896.8U
Authority: CN
Inventors: 钟财王; 陈麦仔; 徐世鸿
Original assignee: Chung Yuan Christian University
Current assignee: Chung Yuan Christian University
Priority date: 2019-06-17
Filing date: 2019-06-17
Publication date: 2020-05-12
Anticipated expiration: 2029-06-17

Abstract

The utility model provides a small molecule biomass extraction and purification device, comprising a raw material liquid premixing device, an extraction device, an extract collection device, a membrane microfiltration device, a degradation device and a membrane ultrafiltration device. The raw material liquid premixing device is filled with biomass raw material liquid. The extraction device is connected to the raw material liquid premixing device, and is used to extract the extract from the biomass raw material liquid. The extract collection device is connected to the extraction device, and is used to store the extract. The membrane microfiltration device is connected to the extract collection device, and is used to filter the microfiltration extract from the extract. The degradation device is connected to the membrane microfiltration device, and is used to degrade the microfiltration extract into a degraded extract. The membrane ultrafiltration device is connected to the degradation device, and is used to filter the small molecule biomass extraction and purification liquid from the degraded extract. The small molecule biomass extraction and purification device of the utility model can achieve the effect of continuously producing small molecule biomass extraction and purification liquid.

Description

Micromolecular biomass extraction and purification equipment

Technical Field

The utility model relates to a micromolecule living beings extraction purification equipment especially indicates a micromolecule living beings extraction purification equipment who utilizes extraction technique and membrane filtration technique to change living beings raw materials liquid into micromolecule living beings extraction purification liquid.

Background

Generally, proteins are long chain structures composed of a plurality of amino acids and are essential components of living bodies, and the proteins enter the human body mainly through ingestion of food and drink, and need to be decomposed into peptides by proteases secreted from digestive organs, and even further into biomolecular structures such as amino acids and the like, so that the proteins can be utilized by the human body. Saponins, triterpenes, polyphenols and the like in phytochemicals are small molecular biomass beneficial to human bodies, and the antioxidant function of the phytochemicals is a main appeal of most of the products.

More specifically, the peptide refers to a structure composed of two or more amino acids, wherein a peptide composed of 2 to 9 amino acids is called an oligopeptide, a peptide composed of 10 or more amino acids is called a polypeptide, and a peptide composed of 2 to 4 amino acids is called a small-molecule peptide because the molecular weight is less than 5k Da (Dalton). Other common small molecule biomass such as phytoxanthin saponin contained in Sapindus mukorossi Gaertn, which also has a molecular weight below 1k Da, is also one of the so-called small molecule biomass.

The peptide care product commonly available in the market generally refers to oligopeptides with less than 10 amino acids, and the peptide care product mainly degrades protein raw materials to generate small molecular peptides with the molecular weight of less than 5k Da, however, the protein raw materials are generally qualified as the small molecular peptide care product after being degraded, but actually, the degraded protein liquid contains the small molecular peptides with the molecular weight of less than 5k Da, and also contains macromolecular peptides with the molecular weight of more than 5k Da, even 20k Da. For example, after the sericin is degraded, about 10% of small molecular peptides smaller than 5k Da are contained, and the remaining 90% of the small molecular peptides contain polypeptides of 10k Da to 20k Da, so that although the sericin is nominally a small molecular peptide care product, when a user actually uses the sericin, the small molecular peptides are not contained, and the small molecular peptides are blocked in skin pores due to the existence of the large molecular peptides, so that the absorption of the small molecular peptides is influenced, and the care product cannot achieve the due efficacy.

SUMMERY OF THE UTILITY MODEL

In view of the fact that in the prior art, the protein liquid labeled as the small molecule peptide care product usually contains a small amount of small molecule peptides and a large amount of large molecule peptides, when a user applies the protein liquid on the skin, the number of the small molecule peptides which can be really absorbed through pores of the skin is limited, and the absorption of the small molecule peptides is easily influenced due to the blockage of the large molecule peptides, and other soapberry water extract liquid contains amino acids, protein liquid, polysaccharide and other impurities or larger molecules besides saponins, and the absorption of the saponins is also influenced; therefore, the main objective of the present invention is to provide a small molecule biomass extraction and purification apparatus for producing a small molecule biomass extraction and purification solution.

The utility model solves the problems of the prior art and adopts the necessary technical means of providing a micromolecule biomass extraction and purification device which is used for converting a biomass raw material liquid into a micromolecule biomass extraction and purification liquid, the extraction and purification equipment for micromolecular biomass comprises a raw material liquid pre-mixing device filled with the biomass raw material liquid, a device for receiving the biomass raw material liquid, the device comprises an extraction device for extracting the biomass raw material liquid to generate an extraction liquid, an extraction liquid collection device for collecting and storing the extraction liquid, a membrane microfiltration device for performing membrane microfiltration on the extraction liquid to separate a microfiltration extraction liquid, a degradation device for degrading the microfiltration extraction liquid to obtain a degradation extraction liquid, and a membrane ultrafiltration device for performing membrane ultrafiltration on the degradation extraction liquid to separate the micromolecular biomass extraction purification liquid.

The extraction device of the extract is communicated with the raw material liquid pre-mixing device. The extraction liquid collecting device is communicated with the extraction device. The membrane micro-filtration device is communicated with the extraction liquid collecting device. The degradation device is communicated with the film micro-filtration device. The membrane ultrafiltration device is communicated with the degradation device.

In an auxiliary technical means derived from the above-mentioned necessary technical means, the extraction and purification equipment for small molecule biomass further comprises a desalination device connected to the membrane ultrafiltration device. Preferably, the equipment for extracting and purifying micromolecular biomass further comprises a storage tank for extracting and purifying micromolecular biomass liquid, and the storage tank is communicated with the desalting device.

In an auxiliary technical means derived from the above-mentioned essential technical means, the extraction apparatus further comprises an extraction tank, a first extract extraction pipe and a first spring filter, wherein the first extract extraction pipe is communicated with the extraction tank, and the first spring filter is disposed in the first extract extraction pipe.

In an auxiliary technical means derived from the above-mentioned essential technical means, the extraction liquid collecting device further comprises a liquid collecting tank, a second extraction liquid extraction pipeline and a second spring filter, wherein the second extraction liquid extraction pipe is communicated with the liquid collecting tank, and the second spring filter is disposed in the second extraction liquid extraction pipeline.

The utility model discloses a micromolecule living beings extraction purification equipment utilizes the extraction device to carry out preliminary extraction to living beings raw materials liquid earlier, then recycle film microfiltration device and filter the microfiltration extract, recycle degradation device makes the microfiltration extract degrade into degradation extract after that, utilize film ultrafiltration device to isolate micromolecule living beings at last, and then the content of effectual reduction macromolecule living beings, the purity of relative improvement micromolecule living beings, and then when in-service use, reduce the possibility that receives macromolecule living beings to block, by the absorption rate that improves micromolecule living beings. Therefore, the micromolecule biomass extraction and purification equipment of the utility model can achieve the effect of continuously producing micromolecule biomass extraction and purification liquid.

The present invention will be further described with reference to the following embodiments and accompanying drawings.

Drawings

Fig. 1 is a schematic plan view showing a raw material liquid pre-mixing device, an extraction device, an extract liquid collection device and a residue discharge device of a small molecule biomass extraction and purification apparatus according to a preferred embodiment of the present invention;

FIG. 2 is a schematic plan view showing a biomass solution filled in a pre-mixing tank in the extraction and purification apparatus for small molecule biomass according to the preferred embodiment of the present invention;

FIG. 3 is a schematic plan view showing the feeding of the biomass feedstock solution in the premixing tank to the extraction tank in the small molecule biomass extraction and purification apparatus according to the preferred embodiment of the present invention;

fig. 4 is a schematic plan view showing that in the extraction and purification apparatus for small molecule biomass provided in the preferred embodiment of the present invention, the extract liquid extracted from the extraction tank is transported to the liquid collection tank; and

fig. 5 is a schematic plan view showing a membrane micro-filtration device, a degradation device, a membrane ultra-filtration device, a desalination device and a storage tank for extracting and purifying solution of small molecular biomass of the small molecular biomass extraction and purification apparatus according to the preferred embodiment of the present invention.

[ notation ] to show

1 raw material liquid pre-mixing device

11 premixing tank

12 first pump

13 first pipeline

14 raw material liquid extraction pipeline

141 first control valve

2 extraction device

21 extraction tank

22 stirrer

23 first extract liquid extraction pipeline

231 second control valve

24 first spring filter

25 second pump

26 first return line

261 first return flow control valve

3 extraction liquid collecting device

31 catch basin

32 transfusion tube

33 third pump

34 second extract liquid extraction pipeline

35 second spring Filter

36 fourth pump

37 extraction liquid conveying pipeline

38 second return line

381 second return flow control valve

4 residue discharge device

41 discharge switch

42 conveyor

43 residue collecting tank

5 film micro-filtration device

51 fifth Pump

52 first Membrane microfilter

53 second Membrane microfilter

54 third Membrane microfilter

55 fourth film microfilter

6 degradation device

7 film ultrafiltration device

71 sixth Pump

72 film ultrafilter

73 pressure detector

74 pH value detector

75 computing device

8 desalination device

9 micromolecule biomass extraction purification liquid storage tank

200 biomass raw material liquid

300 extraction liquid

Detailed Description

Referring to fig. 1 to 5, fig. 1 is a schematic plan view illustrating a raw material liquid pre-mixing device, an extraction device, an extract liquid collection device and a residue discharge device of a small molecule biomass extraction and purification apparatus according to a preferred embodiment of the present invention; FIG. 2 is a schematic plan view showing a biomass solution filled in a pre-mixing tank in the extraction and purification apparatus for small molecule biomass according to the preferred embodiment of the present invention; FIG. 3 is a schematic plan view showing the feeding of the biomass feedstock solution in the premixing tank to the extraction tank in the small molecule biomass extraction and purification apparatus according to the preferred embodiment of the present invention; fig. 4 is a schematic plan view showing that in the extraction and purification apparatus for small molecule biomass provided in the preferred embodiment of the present invention, the extract liquid extracted from the extraction tank is transported to the liquid collection tank; fig. 5 is a schematic plan view showing a membrane micro-filtration device, a degradation device, a membrane ultra-filtration device, a desalination device and a storage tank for extracting and purifying solution of small molecular biomass of the small molecular biomass extraction and purification apparatus according to the preferred embodiment of the present invention.

As shown in fig. 1 to 5, an extraction and purification apparatus for small molecule biomass (i.e. the combination of fig. 1 and 5) comprises a raw material liquid pre-mixing device 1, an extraction device 2, an extract liquid collection device 3, a residue discharge device 4, a membrane micro-filtration device 5, a degradation device 6, a membrane ultra-filtration device 7, a desalination device 8, and a storage tank 9 for extracting and purifying small molecule biomass.

The raw material liquid pre-mixing device 1 comprises a pre-mixing tank 11, a first pump 12, a first pipeline 13 and a raw material liquid extraction pipeline 14. The premixing tank 11 is filled with a biomass raw material liquid 200. The first pump 12 is connected to the ready-mix tank 11, and the first pipe 13 is connected to the first pump 12. The raw material liquid extraction line 14 is connected to the first pump 12 and the pre-mixing tank 11, and has a first control valve 141. The first pump 12 is for sending out the biomass raw material liquid 200 in the premixing tank 11 through the first pipe 13.

The extraction apparatus 2 comprises an extraction tank 21, a stirrer 22, a first extract extraction line 23, a first spring filter 24, a second pump 25 and a first return line 26. The extraction tank 21 is connected to the pre-mixing tank 11 through a first pipeline 13 for receiving the biomass raw material liquid 200 and extracting the biomass raw material liquid 200 with an extraction solvent, so that the biomass in the biomass raw material liquid 200 is separated to generate an extract 300. In practice, the casing of the extraction tank 21 is a double-layer stainless steel structure, and a heating pipe or a coil is disposed in the interlayer of the double-layer stainless steel structure for adjusting the extraction temperature.

The agitator 22 is disposed in the extraction tank 21 for agitating the biomass feedstock 200 and the extraction solvent contained in the extraction tank 21, thereby extracting biomass from the biomass feedstock 200 to generate an extract 300. Practically, the extraction tank 21 can control the proceeding speed of the extraction reaction by adjusting the rotation speed of the stirrer 22. The first extract liquid pumping pipeline 23 is connected to the extraction tank 21 for pumping the extract liquid 300 out of the extraction tank 21, and the first extract liquid pumping pipeline 23 has a second control valve 231 for controlling whether the first extract liquid pumping pipeline 23 is connected or not.

The first spring filter 24 is disposed at the liquid-extracting end of the first extracting liquid-extracting line 23, and is used for filtering the extracting liquid 300 entering the first extracting liquid-extracting line 23. In practice, the first spring filter 24 is a stainless steel spring ring, which can adjust the gap of the spring ring by tightening or loosening, when the stainless steel spring ring is tightened, the residue in the biomass raw material liquid 200 can be effectively filtered, and when the stainless steel spring ring is loosened, the attached residue can be loosened to the bottom of the extraction tank 21 during filtering. In the present embodiment, the first spring filter 24 filters the residue of the extraction liquid, such as dregs.

As mentioned above, the extraction solvent used in the extraction device 2 includes reagents such as water, surfactant, reducing agent, denaturant, enzyme, alkali solution or buffer solution, and in this embodiment, the biomass raw material liquid 200 is, for example, obtained by dissolving sericin obtained by washing and separating silkworm cocoons in water, and includes other impurities such as colloid in addition to protein; in addition, the sapindus mukorossi aqueous extract contains impurities such as amino acids, proteins, and polysaccharides in addition to saponins, and thus can extract biomass such as proteins after extraction with an extraction solvent. In addition, in practice, when the extraction device 2 performs the extraction operation, an acid-base neutralization reaction is performed as necessary, so as to adjust the acid-base value of the extraction liquid. The second pump 25 is connected to the extraction tank 21 through a first return line 26, and the first return line 26 is provided with a first return control valve 261.

The extract collecting device 3 comprises a liquid collecting tank 31, a liquid transporting pipe 32, a third pump 33, a second extract pumping pipe 34, a second spring filter 35, a fourth pump 36, an extract transporting pipe 37 and a second return pipe 38.

The collecting tank 31 is used for collecting and storing the extract 300. The liquid delivery pipe 32 is connected to the liquid trap 31. The third pump 33 is connected to the first extraction liquid extraction line 23 and the liquid transfer line 32. The second extract pumping line 34 is connected to the liquid collecting tank 31, and the second extract pumping line 34 is further connected to the second pump 25, so that the user can pump the extract 300 back to the extraction tank 21 by the operation of the second pump 25 and the first reflux control valve 261. The second spring filter 35 is disposed at the liquid-extracting end of the first extracting liquid-extracting line 23, and is used for filtering the extracting liquid 300 entering the first extracting liquid-extracting line 23. The fourth pump 36 is connected to the second extract pumping line 34, and is used for pumping the extract 300 out of the liquid collecting tank 31 through the second extract pumping line 34. The extract liquid conveying pipeline 37 is connected to the fourth pump 36, and is used for receiving the extract liquid 300 pumped out from the liquid collecting tank 31 by the fourth pump 36 through the second extract liquid pumping pipeline 34, and conveying the extract liquid 300. The second return line 38 is connected to the bottom of the sump 31 and connected to the first pump 12 through the raw material liquid extraction line 14, and the second return line 38 is provided with a second return control valve 381 for controlling whether the extract 300 in the sump 31 flows back to the pre-mix tank 11 or not.

The residue discharge device 4 includes a discharge switch 41, a conveyor 42, and a residue collection tank 43. The discharge switch 41 is connected to the bottom of the extraction tank 21 and controls whether or not the raw material residue accumulated in the bottom of the extraction tank 21 passes through. The conveyor 42 is connected to the discharge switch 41, and when the discharge switch 41 is turned on, the raw material residue at the bottom of the extraction tank 21 enters the conveyor 42 through the discharge switch 41. The residue collection tank 43 opens against the conveyor 42 to receive the raw residue conveyed by the conveyor 42. In the present embodiment, the conveyor 42 is connected to the bottom of the extraction tank 21 through a pipeline, and the discharge switch 41 is disposed between the bottom of the extraction tank 21 and the conveyor 42 to control whether the raw material residue enters the conveyor 42; in addition, a vibrator may be disposed in the pipe between the conveyor 42 and the extraction tank 21 to vibrate the raw material residue accumulated on the bottom of the extraction tank 21 to the conveyor 42, and the conveyor 42 may be a screw conveyor in practice.

The membrane micro-filtration device 5 comprises a fifth pump 51, a first membrane micro-filter 52, a second membrane micro-filter 53, a third membrane micro-filter 54 and a fourth membrane micro-filter 55. The fifth pump 51 is connected to the extract liquid conveying pipeline 37 for pumping out the extract liquid 300 entering the extract liquid conveying pipeline 37. The first membrane micro-filter 52 is connected to the fifth pump 51, the second membrane micro-filter 53 is connected to the first membrane micro-filter 52, the third membrane micro-filter 54 is connected to the second membrane micro-filter 53, and the fourth membrane micro-filter 55 is connected to the third membrane micro-filter 54; when the fifth pump 51 sends the extract 300 into the first membrane micro-filter 52, the second membrane micro-filter 53, the third membrane micro-filter 54 and the fourth membrane micro-filter 55, a micro-filtered extract is separated according to the membrane micro-filtration of the extract 300. In addition, the pore diameters of the filter membranes of the first, second, third and

fourth membrane micro-filters

52, 53, 54 and 55 are, for example, between 1.00 μm and 0.05 μm, and the pore diameters of the filter membranes in the first, second, third and

fourth membrane micro-filters

52, 53, 54 and 55 are arranged from large to small, so as to prevent the membrane pores from being blocked, thereby effectively filtering out particulate impurities in the extract 300.

The degradation device 6 is connected to the fourth membrane microfilter 55 of the membrane microfiltration device 5 for receiving the microfiltration extract separated by the membrane microfiltration device 5, and if a part of the processes needs to be further degraded to generate more micromolecular biomass, the degradation device 6 can be selected to degrade the microfiltration extract to obtain a degradation extract. In this embodiment, the degradation device 6 is a tank-type microwave device, so that the biomass in the microfiltration extract is degraded into a plurality of macromolecular biomasses with molecular weights over 5kDa and a plurality of micromolecular biomasses with molecular weights below 5kDa by using the energy of microwaves; however, in other embodiments, the degradation device 6 is not limited to a microwave device, and may be a tank-type heating or catalytic hydrolysis device. In other embodiments, the small molecule biomass extraction and purification equipment may not include the degradation device 6 if the degradation is not performed.

The membrane ultrafiltration device 7 comprises a sixth pump 71, a membrane ultrafiltration device 72, a pressure detector 73, an pH detector 74 and a calculation device 75. The sixth pump 71 is connected to the degradation device 6 for pumping out the degradation extract entering the degradation device 6. The membrane ultrafilter 72 is connected to the sixth pump 71, and is configured to receive the degradation extract pumped by the sixth pump 71, and further perform membrane ultrafiltration on the degradation extract to generate a small molecule biomass extraction purification solution. In this embodiment, the membrane ultrafiltration device 7 performs filtration by using an ultrafiltration membrane such as a flat plate or hollow fiber having a pore size of 0.01 μm or less, so that the molecular weight of the small molecular biomass in the extraction and purification solution of the small molecular biomass is concentrated between 100Da and 3000 Da. In addition, the ultrafiltration membrane used in the membrane ultrafiltration device 7 can be selected by the pore size or membrane material characteristics to filter out small molecular biomass with molecular weight concentrated between 100Da and 3000Da, and avoid the problem of blockage caused by rapid deposition of biomass on the membrane material surface.

The pressure detector 73 is disposed at the inlet and outlet of the membrane ultrafilter 72 for measuring the pressure variation value passing through the membrane ultrafilter 72. The pH detector 74 is connected to the membrane ultrafilter 72 for detecting the pH of the purified liquid extracted from the small molecule biomass. The calculating device 75 is electrically connected to the pressure detector 73 and the ph detector 74, and is configured to receive the pressure variation detected by the pressure detector 73 and the ph detected by the ph detector 74.

The desalination device 8 is connected to the ph detector 74 for receiving the purified liquid extracted from the small molecular biomass, and filtering the salts generated when the purified liquid extracted from the small molecular biomass is subjected to an acid-base neutralization reaction. In practical use, the desalination apparatus 8 may be used to remove salts by means of a membrane, a packed tube, a liquid chromatography system, gravity flow, or centrifugation, and may further adjust the pH. However, in other embodiments, the small molecule biomass extraction and purification apparatus may not include the desalination device 8 if acid-base neutralization is not required.

The storage tank 9 for extracting and purifying the liquid from the micromolecular biomass is communicated with the desalting device 8 and is used for receiving and storing the purified liquid from the micromolecular biomass passing through the desalting device 8.

In summary, compared with the protein liquid of the small molecule peptide care product in the prior art, the protein liquid usually contains a small amount of small molecule peptides and a large amount of large molecule peptides, so when a user applies the protein liquid on the skin, the absorption of the small molecule peptides is easily affected because the large molecule peptides block the pores of the skin; the utility model discloses a micromolecule living beings extraction purification equipment utilizes the extraction device to carry out preliminary extraction to living beings raw materials liquid earlier, then recycle film microfiltration device and filter the microfiltration extract, recycle degradation device makes the microfiltration extract degrade into degradation extract after that, utilize film ultrafiltration device to isolate micromolecule living beings most, and then the content of effectual reduction macromolecule living beings, the purity of relative improvement micromolecule living beings, and then when in-service use, reduce the possibility that receives macromolecule living beings to block, by the absorption rate that improves micromolecule living beings. Therefore, the micromolecule biomass extraction and purification equipment of the utility model can really achieve the effect of continuously producing micromolecule biomass extraction and purification liquid.

The foregoing detailed description of the preferred embodiments is intended to more clearly illustrate the features and spirit of the present invention, and is not intended to limit the scope of the invention by the preferred embodiments disclosed above. On the contrary, it is intended to cover various modifications and equivalent arrangements included within the scope of the appended claims.

Claims

1. a small-molecule biomass extraction and purification equipment, for converting biomass feedstock liquid into small-molecule biomass extraction and purification liquid, it is characterized in that, comprises:

a raw material liquid pre-mixing device filled with the biomass raw material liquid;

an extraction device for receiving the biomass raw material liquid and extracting the biomass raw material liquid to generate an extract, which is communicated with the raw material liquid pre-mixing device;

In order to collect and store the extractive liquid collecting device of the extracting liquid, it is communicated with the extracting device:

A membrane microfiltration device for performing membrane microfiltration on the extract to separate the microfiltration extract, communicated with the extract collection device;

A degradation device for degrading the microfiltration extract to obtain a degraded extract, communicated with the membrane microfiltration device; and

A membrane ultrafiltration device used for performing membrane ultrafiltration on the degradation extract to separate the small molecular biomass extraction and purification solution is connected to the degradation device.

2 . The small molecule biomass extraction and purification equipment according to claim 1 , further comprising a desalination device, which is connected to the membrane ultrafiltration device. 3 .

3 . The small-molecule biomass extraction and purification equipment according to claim 2 , further comprising a small-molecule biomass extraction and purification liquid storage tank, which is communicated with the desalination device. 4 .

4. The small molecule biomass extraction and purification equipment according to claim 1, wherein the extraction device further comprises an extraction tank, a first extraction liquid extraction pipeline and a first spring filter, and the first extraction The effluent extraction pipe is communicated with the extraction tank, and the first spring filter is arranged in the first extraction liquid extraction pipeline.

5. The small molecule biomass extraction and purification equipment according to claim 1, wherein the extractive liquid collecting device further comprises a liquid collecting tank, a second extractive liquid extraction pipeline and a second spring filter, so The second extraction liquid extraction pipe is communicated with the liquid collecting tank, and the second spring filter is arranged in the second extraction liquid extraction pipeline.