CN219603575U

CN219603575U - Heating assembly of fluorescent quantitative PCR instrument

Info

Publication number: CN219603575U
Application number: CN202320756164.1U
Authority: CN
Inventors: 江涛; 刘顺珍; 赵小港; 赵波
Original assignee: Suzhou Laiboyi Medical Technology Co ltd
Current assignee: Suzhou Laiboyi Medical Technology Co ltd
Priority date: 2023-04-07
Filing date: 2023-04-07
Publication date: 2023-08-29
Anticipated expiration: 2033-04-07

Abstract

The utility model discloses a heating component of a fluorescence quantitative PCR instrument, which comprises a mounting plate, a heat dissipation component and a heating component, wherein the heating component and the heat dissipation component are arranged on the mounting plate; the heat dissipation assembly comprises a fan and heat dissipation fins, a round hole is formed in the mounting plate, and the fan is detachably arranged at the bottom of the mounting plate and corresponds to the round hole; the radiating fins are detachably arranged above the mounting plate and correspond to the round holes; the heating assembly comprises a heat seat, a heat seat pressing plate, a PI heating film, a heat conducting fin and a heat preservation box, wherein the heat preservation box is arranged on the radiating fin, an opening is arranged below the heat preservation box, a TEC thermoelectric refrigerating piece is arranged in the heat preservation box, and the TEC thermoelectric refrigerating piece is positioned above the opening; the utility model aims to solve the problems of weak specificity and low analysis sensitivity of the PCR instrument for detecting the liquid to be detected in the prior art.

Description

Heating assembly of fluorescent quantitative PCR instrument

Technical Field

The utility model relates to the technical field of PRC instruments, in particular to a heating component of a fluorescent quantitative PCR instrument.

Background

In the field of molecular biology, the polymerase chain reaction technique (Polymerase Chain Reaction, abbreviated as PCR) is a commonly used experimental technique whose basic principle is similar to the natural replication process of DNA, and is a method for in vitro enzymatic promotion of the synthesis of specific DNA fragments, whose specificity depends on oligonucleotide primers complementary to both ends of a target sequence. The PCR is basically formed by three basic reaction steps of deformation, annealing and extension, has the advantages of strong specificity, high sensitivity, simple and convenient operation, time saving and the like, can be used for basic researches such as gene separation, cloning, nucleic acid sequence analysis and the like, and is more and more widely applied to diagnosis of clinical diseases. The instrument for realizing the real-time fluorescent quantitative PCR technology is called a real-time fluorescent quantitative PCR instrument, is essentially a temperature control device, and can accurately control the denaturation temperature, the renaturation temperature and the extension temperature. The basic principle is as follows: the light source lamp emits excitation light, the excitation light reaches the reaction tube with the sample through the optical component, and the sample emits fluorescence after being excited. The excited fluorescence then passes through the optical assembly to the detector. The filter is a component of the optical assembly that allows light of a specific wavelength to pass through, thereby achieving the purpose of distinguishing products. The fluorescence passing through the filter lens is captured by the detector after passing through the optical component, and the captured fluorescence is input into a computer for calculation and analysis after a series of photoelectric conversion. Common detectors for fluorescent PCR are cameras, PMTs (photomultiplier tubes), photodiodes, etc.

In a traditional fluorescence detection system, excitation light is emitted through a light source, the excitation light is excited after passing through an incident light filter, and the excited fluorescence passes through a detection light filter and then reaches a detector (such as a photodiode and a camera). However, whether a photodiode or a camera is used as a detector, a filter is used to obtain fluorescence of a desired wavelength. The multi-channel fluorescence detection needs a plurality of filters, and fluorescence with different wavelengths is selected or the filters are rotated through the filters; or various filters are mounted on an optical head, and scanned hole by the back and forth movement of the optical head. Both of these filtering modes require mechanical movement means, otherwise the required fluorescence is not obtained. The mechanical moving structure (device) is introduced, so that the instrument is complex and huge, and the cost is high; more importantly, the motion makes the whole instrument in a dynamic detection system, equipment faults are extremely easy to cause, and huge cost and burden are brought to subsequent maintenance. In addition, the optics of a conventional PCR instrument is typically located at the top of the instrument. The excitation light source projects the sample to be detected in the sample cell after passing through the incident filter, and the fluorescence excited by the sample to be detected is directly projected onto a photosensitive receiving tube or a photoelectric detector such as a CCD after being filtered by the detection light filter, so that the target of quantitative detection of the sample to be detected is realized. Excitation light irradiates the sample tube from the top of the sample base to excite fluorescence and realize detection, the method has particularly high requirements on the quality of the caps of the sample reaction tube and the reagent plate, has high transmittance, and is particularly easy to cause optical path confusion.

Disclosure of Invention

The utility model provides a heating component of a fluorescent quantitative PCR instrument, and aims to solve the problems that the PCR instrument in the prior art is weak in specificity of liquid detection to be detected and low in analysis sensitivity.

In order to solve the technical problems, the utility model adopts the following technical scheme:

the heating component of the fluorescent quantitative PCR instrument comprises a mounting plate, a heat dissipation component and a heating component, wherein the heating component and the heat dissipation component are arranged on the mounting plate;

the heat dissipation assembly comprises a fan and heat dissipation fins, a round hole is formed in the mounting plate, and the fan is detachably arranged at the bottom of the mounting plate and corresponds to the round hole; the radiating fins are detachably arranged above the mounting plate and correspond to the round holes;

the heating assembly comprises a heat seat, a heat seat pressing plate, a PI heating film, a heat conducting fin and a heat preservation box, wherein the heat preservation box is arranged on the radiating fin, an opening is arranged below the heat preservation box, a TEC thermoelectric refrigerating piece is arranged in the heat preservation box, and the TEC thermoelectric refrigerating piece is positioned above the opening; the heat conducting fin is arranged above the TEC thermoelectric refrigerating fin, the PI heating film is arranged on the heat conducting fin, the heat seat is arranged on the PI heating film, the heat seat pressing plate is used for pressing the heat seat on the PI heating film, and the heat seat pressing plate is detachably connected with the radiating fin; the hot seat pressing plate is provided with a plurality of optical fibers, the hot seat is provided with a plurality of detection holes, and the position of each optical fiber corresponds to the position of one detection hole.

Further, the mounting plate is of a channel steel-shaped structure.

Further, two PI heating films are arranged, and the two PI heating films are arranged on the heat conducting fin; the number of the heat seats corresponds to the number of the PI heating films, and each PI heating film is provided with one heat seat.

Further, two limiting protrusions are arranged on the heat preservation box, an optical fiber fixing block is arranged between the two limiting protrusions, and a plurality of optical fibers penetrate through the optical fiber fixing block and the heat preservation box and then are arranged on the hot seat pressing block.

Further, two wiring grooves are formed in the channel-shaped mounting plate, a plurality of optical fibers are arranged inside the wiring grooves, two pressing blocks are arranged on the mounting plate and located above the wiring grooves, and the pressing blocks are used for fixing the optical fibers in the wiring grooves.

Further, the fan and the radiating fins are detachably connected with the mounting plate through bolts.

Further, the four corners of mounting panel bottom all are provided with the pillar.

Compared with the prior art, the utility model has the following beneficial effects:

the utility model mainly comprises a mounting plate, a heat radiation component and a heating component, wherein the heating component and the heat radiation component are arranged on the mounting plate; in actual use, the staff opens PI heating film for PI heating film generates heat, make the conducting strip carry out heat conduction after PI heating film generates heat, and then heat the inside liquid of waiting to detect of hot seat, after the heating, the staff opens TEC thermoelectric cooling piece, TEC thermoelectric cooling piece refrigerates the hot seat, through carrying out intermittent type formula heating and refrigeration to the inside liquid of waiting to detect of hot seat, make the inside liquid of waiting to detect of hot seat fully react, the detection hole on the hot seat is aimed at to optic fibre, detect the inside liquid of waiting to detect of hot seat, the benefit of setting like this is through carrying out intermittent type formula heating and refrigeration to the inside liquid of waiting to detect of hot seat makes the PCR appearance stronger to the detection specificity of the inside liquid of waiting to detect of hot seat, the analysis sensitivity is higher, the effect of fan and fin is when heating the hot seat carries out heat transfer.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present utility model, the drawings that are needed in the embodiments will be briefly described below, it being understood that the following drawings only illustrate some examples of the present utility model and therefore should not be considered as limiting the scope, and other related drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.

Fig. 1 is a schematic structural view of the present utility model.

Fig. 2 is an exploded view of the present utility model.

In the figure, 1-mounting plate, 2-hot seat, 3-hot seat pressing plate, 4-PI heating film, 5-heat conducting fin, 6-radiating fin, 7-optical fiber, 8-fan, 9-heat preservation box, 10-TEC thermoelectric cooling plate, 11-briquetting, 12-pillar, 13-optical fiber fixing block, 14-limit bulge and 15-wiring groove.

Detailed Description

The present utility model is further described below in conjunction with embodiments, which are merely some, but not all embodiments of the present utility model. Based on the embodiments of the present utility model, other embodiments that may be used by those of ordinary skill in the art without making any inventive effort are within the scope of the present utility model.

Example 1

Referring to fig. 1 and 2, the present embodiment discloses a heating component of a fluorescent quantitative PCR apparatus, including a mounting plate 1, a heat dissipation component and a heating component, wherein the heating component and the heat dissipation component are both disposed on the mounting plate 1;

the heat radiation component comprises a fan 8 and heat radiation fins 6, a round hole is formed in the mounting plate 1, and the fan 8 is detachably arranged at the bottom of the mounting plate 1 and corresponds to the round hole; the radiating fins 6 are detachably arranged above the mounting plate 1 and correspond to the round holes;

the heating assembly comprises a heat seat 2, a heat seat pressing plate 3, a PI heating film 4, a heat conducting fin 5 and a heat preservation box 9, wherein the heat preservation box 9 is arranged on the heat radiating fin 6, an opening is arranged below the heat preservation box 9, a TEC thermoelectric cooling piece 10 is arranged inside the heat preservation box 9, and the TEC thermoelectric cooling piece 10 is arranged above the opening; the heat conducting fin 5 is arranged above the TEC thermoelectric refrigerating fin 10, the PI heating film 4 is arranged on the heat conducting fin 5, the heat seat 2 is arranged on the PI heating film 4, the heat seat pressing plate 3 is used for pressing the heat seat 2 on the PI heating film 4, and the heat seat pressing plate 3 is detachably connected with the radiating fin; the hot seat pressing plate 3 is provided with a plurality of optical fibers 7, the hot seat 2 is provided with a plurality of detection holes, and the position of each optical fiber 7 corresponds to the position of one detection hole;

in the practical use process, a worker starts the PI heating film 4 to heat the PI heating film 4, the heat conducting sheet 5 conducts heat after the PI heating film 4 heats the liquid to be detected in the heat seat 2, after the heating is finished, the worker starts the TEC thermoelectric cooling sheet 10, the TEC thermoelectric cooling sheet 10 refrigerates the heat seat 2, the liquid to be detected in the heat seat 2 fully reacts through intermittent heating and refrigeration of the liquid to be detected in the heat seat 2, the optical fiber 7 is aligned to the detection hole on the heat seat 2 to detect the liquid to be detected in the heat seat 2, and the advantages are that the PCR instrument is enabled to have stronger detection specificity of the liquid to be detected in the heat seat 2 through intermittent heating and refrigeration of the liquid to be detected in the heat seat 2, the analysis sensitivity is higher, and the fan 8 and the cooling fin are used for exchanging heat when the heat seat 2 is heated.

Further, the mounting plate 1 is of a channel steel-shaped structure, and the mounting plate 1 of the channel steel-shaped structure can save mounting space during mounting and is more convenient for mounting the radiating fins.

Further, there are two PI heating films 4, and both PI heating films 4 are disposed on the heat conductive sheet 5; the number of the heat seats 2 corresponds to the number of the PI heating films 4, one heat seat 2 is arranged on each PI heating film 4, in the actual use process, the two PI heating films 4 are connected with each other, and the two PI heating films 4 heat and uniformly heat the two heat seats 2.

Further, two limiting protrusions 14 are arranged on the heat preservation box 9, an optical fiber fixing block 13 is arranged between the two limiting protrusions 14, a plurality of optical fibers 7 penetrate through the optical fiber fixing block 13 and the heat preservation box 9 and then are arranged on the pressing block 11 of the hot base 2, the optical fiber fixing block 13 is fixed by the aid of the limiting protrusions 14, a plurality of small holes are formed in the optical fiber fixing block 13, one optical fiber 7 is arranged in each small hole, and the optical fiber 7 is fixed by the aid of the optical fiber fixing block 13.

Further, be provided with two wiring grooves 15 on the mounting panel 1 of channel-section steel form, a plurality of optic fibre 7 set up inside wiring groove 15, be provided with on the mounting panel 1 with two briquetting 11, briquetting 11 is located the top of wiring groove 15, briquetting 11 is used for fixing a plurality of optic fibre 7 in wiring groove 15, the effect of setting up wiring groove 15 is convenient to comb optic fibre 7 circuit, avoid a plurality of optic fibre 7 to take place the winding, because optic fibre 7 is very easily damaged, so can effectually protect optic fibre 7 after carding a plurality of optic fibre 7.

Further, the fan 8 and the radiating fins 6 are detachably connected with the mounting plate 1 through bolts, and the fan 8 and the radiating fins 6 can be replaced at any time.

Further, the four corners of the bottom of the mounting plate 1 are provided with the supporting posts 12, and the purpose of the supporting posts 12 is to facilitate the mounting of the mounting plate 1.

In the description of the present utility model, it should be understood that the terms "coaxial," "bottom," "one end," "top," "middle," "another end," "upper," "one side," "top," "inner," "front," "center," "two ends," etc. indicate orientations or positional relationships based on the orientation or positional relationships shown in the drawings, are merely for convenience in describing the present utility model and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present utility model.

Furthermore, the terms "first," "second," "third," "fourth," and the like are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated, whereby features defining "first," "second," "third," "fourth" may explicitly or implicitly include at least one such feature.

In the present utility model, unless explicitly specified and limited otherwise, the terms "mounted," "configured," "connected," "secured," "screwed," and the like are to be construed broadly and may be, for example, fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; either directly or indirectly through intermediaries, or in communication with each other or in interaction with each other, unless explicitly defined otherwise, the meaning of the terms described above in this application will be understood by those of ordinary skill in the art in view of the specific circumstances.

Although embodiments of the present utility model have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A heating assembly of a fluorescent quantitative PCR instrument, characterized in that: the heat-dissipating device comprises a mounting plate (1), a heat-dissipating component and a heating component, wherein the heating component and the heat-dissipating component are arranged on the mounting plate (1);

the heat radiation component comprises a fan (8) and heat radiation fins (6), a round hole is formed in the mounting plate (1), and the fan (8) is detachably arranged at the bottom of the mounting plate (1) at the position corresponding to the round hole; the radiating fins (6) are detachably arranged above the mounting plate (1) and correspond to the positions of the round holes;

the heating assembly comprises a hot seat (2), a hot seat pressing plate (3), a PI heating film (4), a heat conducting fin (5) and a heat preservation box (9), wherein the heat preservation box (9) is arranged on the heat radiating fin (6), an opening is formed below the heat preservation box (9), a TEC thermoelectric cooling sheet (10) is arranged inside the heat preservation box (9), and the TEC thermoelectric cooling sheet (10) is positioned above the opening; the heat conducting fin (5) is arranged above the TEC thermoelectric refrigerating fin (10), the PI heating film (4) is arranged on the heat conducting fin (5), the heat seat (2) is arranged on the PI heating film (4), the heat seat pressing plate (3) is used for pressing the heat seat (2) on the PI heating film (4), and the heat seat pressing plate (3) is detachably connected with the radiating fin; the hot seat pressing plate (3) is provided with a plurality of optical fibers (7), the hot seat (2) is provided with a plurality of detection holes, and the position of each optical fiber (7) corresponds to the position of one detection hole.

2. The heating assembly of a fluorescent quantitative PCR instrument of claim 1 wherein: the mounting plate (1) is of a channel steel-shaped structure.

3. The heating assembly of a fluorescent quantitative PCR instrument of claim 1 wherein: two PI heating films (4) are arranged, and the two PI heating films (4) are arranged on the heat conducting sheet (5); the number of the heat seats (2) corresponds to the number of the PI heating films (4), and one heat seat (2) is arranged on each PI heating film (4).

4. The heating assembly of a fluorescent quantitative PCR instrument of claim 2 wherein: two limiting protrusions (14) are arranged on the heat preservation box (9), an optical fiber fixing block (13) is arranged between the two limiting protrusions (14), and a plurality of optical fibers (7) are installed on the pressing block (11) of the heat seat (2) after passing through the optical fiber fixing block (13) and the heat preservation box (9).

5. The heating assembly of a fluorescent quantitative PCR instrument of claim 4, wherein: be provided with two wiring grooves (15) on mounting panel (1) of channel-section steel form, a plurality of optic fibre (7) set up inside wiring groove (15), are provided with on mounting panel (1) with two briquetting (11), and briquetting (11) are located the top of wiring groove (15), and briquetting (11) are used for fixing a plurality of optic fibre (7) in wiring groove (15).

6. The heating assembly of a fluorescent quantitative PCR instrument of claim 1 wherein: the fan (8) and the radiating fins (6) are detachably connected with the mounting plate (1) through bolts.

7. The heating assembly of a fluorescent quantitative PCR instrument of claim 1 wherein: the four corners of the bottom of the mounting plate (1) are provided with struts (12).