CN217791039U - Phototaxis detection device - Google Patents

Abstract

The utility model discloses a phototaxis detection device, which comprises a support piece, a container body, a separator and a test lamp; the container body is arranged on the support and used for containing an animal to be tested; the separator is arranged on the container body in a switchable manner at an opening position and a closing position, and divides the interior of the container body into a light chamber and a dark chamber; in the open position, the light chamber is in communication with the dark chamber; when in the closed position, the light chamber is not communicated with the dark chamber; the test lamp is installed on support piece, and the relative light room setting of light-emitting side of test lamp, and the test lamp is used for transmitting light to the light indoor. The utility model provides a phototaxis detection device can simulate under the natural condition zebra fish juvenile fish or other animals and receive the amazing scene of natural light, can test the phototaxis reaction behavior of fish through changing the environment illumination condition.

Description

Phototaxis detection device

Technical Field

The utility model relates to a life science laboratory glassware technical field especially relates to a phototaxis detection device.

Background

The visual system, the most important sensory nervous system, plays an important role in the survival and reproduction of biological individuals, and a large number of behavioural activities significantly depend on the development of the visual system and the development of visual functions, such as foraging, avoiding predation, reproductive behavior and habitat selection. The correct establishment of the optic nerve circuit will give substantial advantages in terms of adaptability and evolutionary outcome, especially in early life stages, where the precocious perception of food and characters by predators is essential for successful survival and growth. The time at which the visual system matures is compatible with the ecological pressure of avoiding predators and starting voluntary feeding. Abnormalities in vision and vision-mediated behavior caused by exogenous biotoxins are reflected in the inability to escape predators and search for feed, ultimately reducing survival, growth and stability of fish populations. The visual system is often considered a sensitive target for environmental pollutants, and particularly, as fish have no eyelids, and the direct exposure to harmful substances in aquatic environments makes them at higher risk of bioabsorption and bioaccumulation, eye-related research is receiving more and more attention as science and technology advances.

Zebrafish (Danio rerio) is a model animal frequently used in biological experiments, and has numerous advantages: the spawning time span is short, and sperms, ova, embryos and the like are cheap and easy to obtain; the size is small, and a large amount of the feed can be cultured under certain conditions; the embryo is not only transparent and easy to culture, but also can be directly observed under a microscope; the zebrafish genome is highly similar to the human genome and, due to the rapid dysplasia of the visual system of the zebrafish embryo, eye tissue can form at the time of the day of fertilization based on morphological, physiological and behavioral monitoring; three days after fertilization, the vision function can be preliminarily formed, and photosensitivity is generated; one week after fertilization, visual function tends to be perfect. Its use in the systematic study of the visual toxicity of environmental pollutants during early embryonic development has proven to be a valuable teleost model for toxicology studies in the visual system.

Over the past few decades, the variety and amount of chemical substances introduced into the environment has increased, and it has been found through extensive research that embryonic development and visual function of the neuroretina of fish are acutely or chronically exposed to various exogenous substances, and these contaminants can cause multiple defects in the visual system in different levels of biological tissues (e.g., genes, proteins, histology, and physiology), thereby impairing vision-mediated behavior. Due to the complex ecological relevance of visual abnormalities and the prevalence of aquatic pollutants, aquatic toxicology is drawing increasing attention with respect to the visual neuroscience. However, the traditional toxicity research has large workload and long period, and a professional experimental device is lacked at present to conveniently and quickly test the zebra fish juvenile fish with the visual function of normal growth and development.

SUMMERY OF THE UTILITY MODEL

The utility model aims at providing a phototaxis detection device aims at realizing that zebra fish juvenile fish or other animals receive the amazing scene of natural light under the simulation natural condition, carries out extensive, accuracy, screening conveniently from behaviouristics to the zebra fish juvenile fish that has visual defect on the scientific research level, remains and cultivates high-quality fry, promotes fish species optimization on agricultural cultivation.

In order to achieve the above object, the utility model provides a phototaxis detection device, include:

a support member;

the container body is arranged on the support part and used for accommodating an animal to be tested;

a partition switchably provided on the container body in an open position and a closed position, the partition dividing an inside of the container body into a light chamber and a dark chamber;

in the open position, the light chamber is in communication with the dark chamber;

in the closed position, the light chamber is not in communication with the dark chamber; and

the test lamp, install in on the support piece, just the light-emitting side of test lamp is relative the light room sets up, the test lamp is used for emission light extremely in the light room.

Optionally, the container body includes a container body having a container opening, and a first cover plate and a second cover plate covering the container opening, the first cover plate forming an upper chamber wall of the light chamber, and the second cover plate forming an upper chamber wall of the dark chamber.

Optionally, the first cover plate is movably arranged on the box body along the length direction of the container body; and/or

The second cover plate is movably disposed on the case body along a length direction of the container body.

Optionally, a first sliding groove extending along the length direction of the box opening is formed in the position, close to the box opening, of the inner wall of the box body, and two sides of the first cover plate and two sides of the second cover plate are respectively arranged in the first sliding groove in a sliding mode.

Optionally, the partition is movably disposed at a middle portion of the box body in a height direction of the container body, so that the partition is switchably disposed on the container body in the open position and the closed position.

Optionally, a second sliding groove extending along the height direction of the box body is formed in the inner wall of the middle of the box body, and two sides of the isolating piece are slidably arranged in the second sliding groove.

Optionally, the test lamp is movably disposed on the support in a height direction of the container body.

Optionally, the container body is made of black opaque acrylic; and/or

The length of the container body is 90-110 mm, the width of the container body is 20-40 mm, and the height of the container body is 30-50 mm.

Optionally, the spacer is provided in a plate shape; the length of the isolation piece is 20-30 mm, the width of the isolation piece is 4-5 mm, and the height of the isolation piece is 30-40 mm.

Optionally, the test lamp is an LED lamp.

In the technical scheme of the utility model, the phototaxis detection device comprises a support piece, a container body, a partition piece and a test lamp; the container body is arranged on the support and used for containing an animal to be tested; the separator is arranged on the container body in a switchable manner at an opening position and a closing position, and divides the interior of the container body into a light chamber and a dark chamber; in the open position, the light chamber is in communication with the dark chamber; when in the closed position, the light chamber is not communicated with the dark chamber; the test lamp is installed on support piece, and the relative light room setting in light-emitting side of test lamp, and the test lamp is used for transmitting light to the light indoor. It can be understood that the zebra fish juvenile fish or other animals to be tested can be placed in the container body, the phototaxis reaction behavior of the zebra fish juvenile fish is tested by adjusting the test lamp to change the ambient illumination condition, the scene that the zebra fish juvenile fish or other animals are stimulated by natural light under the simulated natural condition is realized, the zebra fish juvenile fish with visual defects can be screened in a large-scale, accurate and convenient manner from the behaviourology in the scientific research level, high-quality fry can be reserved and cultured in agricultural breeding, and the optimization of fish varieties is promoted.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the embodiments or the prior art descriptions will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the structures shown in the drawings without creative efforts.

Fig. 1 is a first state diagram of a testing process stage in an embodiment of the phototaxis detection apparatus of the present invention;

fig. 2 is a second state diagram of the testing process stage of the phototaxis detection device according to an embodiment of the present invention;

FIG. 3 is a third state diagram of the testing process stage in one embodiment of the phototaxis detection apparatus of the present invention;

FIG. 4 is a fourth state diagram of the testing process stage in one embodiment of the phototaxis detection apparatus of the present invention;

fig. 5 is a fifth state diagram of the testing process stage in an embodiment of the phototaxis detection apparatus of the present invention.

The reference numbers illustrate:

10. a support member; 20. a container body; 30. a spacer; 40. testing the lamp; 200. a box body; 201. a first cover plate; 202. a second cover plate; 20a, an optical chamber; 20b, a darkroom; 20c, a first chute; 20d, a second chute.

The objects, features and advantages of the present invention will be further described with reference to the accompanying drawings.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by a person skilled in the art without making creative efforts belong to the protection scope of the present invention.

It should be noted that, if directional indications (such as up, down, left, right, front, back, 8230; \8230;) are provided in the embodiments of the present invention, the directional indications are only used to explain the relative position relationship between the components, the motion situation, etc. in a specific posture (as shown in the attached drawings), and if the specific posture is changed, the directional indications are correspondingly changed.

In addition, if there is a description relating to "first", "second", etc. in the embodiments of the present invention, the description of "first", "second", etc. is for descriptive purposes only and is not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In addition, if appearing throughout the text, "and/or" is meant to include three juxtaposed aspects, taking "A and/or B" as an example, including either the A aspect, or the B aspect, or both A and B satisfied aspects. In addition, the technical solutions in the embodiments may be combined with each other, but it must be based on the realization of those skilled in the art, and when the technical solutions are contradictory or cannot be realized, the combination of the technical solutions should not be considered to exist, and is not within the protection scope of the present invention.

The utility model provides a phototaxis detection device, applicable phototaxis in detecting fish or other animals, especially zebra fish juvenile fish will use zebra fish juvenile fish to explain in detail as the examination animal of awaiting measuring as the example below, and do not represent the utility model discloses a phototaxis detection device only is applicable to the phototaxis that detects zebra fish juvenile fish.

Referring to fig. 1 to 5, in an embodiment of the present invention, the phototaxis detection device includes a support member 10, a container body 20, a spacer 30, and a test lamp 40; the container body 20 is arranged on the support 10 and used for accommodating an animal to be tested; the partition 30 is switchably provided on the container body 20 at an open position and a closed position, the partition 30 dividing the inside of the container body 20 into a light chamber 20a and a dark chamber 20b; in the open position, light chamber 20a is in communication with dark chamber 20b; in the closed position, light chamber 20a is not in communication with dark chamber 20b; the test lamp 40 is mounted on the support 10, and a light emitting side of the test lamp 40 is disposed opposite to the light chamber 20a, and the test lamp 40 is used for emitting light into the light chamber 20 a.

In this embodiment, the support member 10 may include a bottom plate to which the container body 20 may be fixed and a support bar to which the test lamp 40 may be mounted.

The container body 20 may be rectangular, cylindrical, etc., and the container body 20 may be made of a non-light-transmitting material, preferably black non-light-transmitting acrylic, but not limited thereto.

The isolation member 30 may be a plate-shaped structure, and the material thereof may also be non-light-transmitting material such as black opaque acrylic, etc., which is not limited herein.

The test lamp 40 may be an LED lamp, which can convert light of different colors, including but not limited to white light, red light, green light, and blue light. The LED lamps can provide different illumination conditions for the light chamber 20a to meet the requirement of the phototaxis test.

In the technical scheme of the utility model, the phototaxis detection device comprises a support member 10, a container body 20, a spacer 30 and a test lamp 40; the container body 20 is arranged on the support 10 and is used for accommodating an animal to be tested; a partition 30 switchably provided on the container body 20 at an open position and a closed position, the partition 30 dividing the inside of the container body 20 into a light chamber 20a and a dark chamber 20b; in the open position, light chamber 20a is in communication with dark chamber 20b; in the closed position, light chamber 20a is not in communication with dark chamber 20b; the test lamp 40 is mounted on the support 10, and a light emitting side of the test lamp 40 is disposed opposite to the light chamber 20a, and the test lamp 40 is used for emitting light into the light chamber 20 a. It can be understood that the zebra fish juvenile fish or other animals to be tested can be placed in the container body 20, the phototaxis reaction behavior of the zebra fish juvenile fish is tested by adjusting the test lamp 40 to change the ambient illumination condition, the scene that the zebra fish juvenile fish or other animals are stimulated by natural light under the simulated natural condition is realized, the zebra fish juvenile fish with visual defects can be screened in a large-scale, accurate and convenient manner at the scientific research level from the behavioristics, high-quality fry can be reserved and cultivated in agricultural breeding, and the optimization of the fish variety is promoted.

Referring mainly to fig. 1 and 2, in one embodiment, the container body 20 may include a box body 200 having a box opening, and a first cover plate 201 and a second cover plate 202 covering the box opening, the first cover plate 201 forming an upper chamber wall of the light chamber 20a, and the second cover plate 202 forming an upper chamber wall of the dark chamber 20b. Therefore, the container body 20 can be conveniently opened so as to take and place the zebra fish juvenile fish to be tested, and pure water or culture solution and the like are added into the container body 20, thereby improving the operation convenience of the zebra fish phototaxis test.

To further improve the operational convenience of the test, referring mainly to fig. 1 and 4, in one embodiment, a first cover plate 201 is movably provided on the case 200 in a length direction of the container body 20 to open and close the light chamber 20a; and/or a second cover 202 is movably provided on the case 200 in a length direction of the container body 20 to facilitate opening and closing of the dark room 20b.

In this embodiment, a first sliding slot 20c extending along the length direction of the inner wall of the box body 200 near the box opening may be formed, and two sides of the first cover plate 201 and the second cover plate 202 are respectively slidably disposed in the first sliding slot 20 c. Like this, through adopting sliding construction, realized the slip of first apron 201 and second apron 202 and opened and shut, further promoted the operation convenience.

Likewise, the spacer 30 may also adopt a sliding structure. Referring to fig. 3 and 4, in one embodiment, the barrier 30 is movably disposed at the middle of the container body 20 in the height direction of the container body 200 such that the barrier 30 is switchably disposed on the container body 20 at the open position and the closed position.

Wherein, the inner wall of the middle part of the box body 200 can be provided with a second sliding groove 20d extending along the height direction thereof, and the two sides of the isolating piece 30 are slidably arranged in the second sliding groove 20 d.

It will be appreciated that the partition 30 is free to slide within the second chute 20d and bisects the chamber of the container body 20 into the light chamber 20a and the dark chamber 20b, which maintain fluid communication between the two chambers, and when the partition 30 is closed, the light chamber 20a and the dark chamber 20b are completely separated and the zebra fish larvae cannot pass from one chamber to the other.

In this embodiment, the container body 20 may be made of opaque materials such as black opaque acrylic.

In order to improve convenience in adjusting ambient light conditions, referring to fig. 1, in one embodiment, the test lamp 40 may be movably disposed on the support member 10 in a height direction of the container body 20.

In this embodiment, the test lamp 40 may be disposed on the supporting member 10 through a lifting platform, and the lifting platform may be made of an aluminum alloy, which is not limited herein.

In this embodiment, the test lamp 40 can be adjusted to a position about 15cm directly above the light chamber 20a so as to emit light rays meeting the test requirements to the light chamber 20a, thereby improving the test accuracy, and simultaneously enabling the volume of the whole phototaxis detection device to tend to be miniaturized, thereby facilitating the operation of a tester.

In the behavioral testing experiment for visual induction of zebra fish, in order to ensure the accuracy of the test detection, the phototaxis detection device system is placed in a horizontal, quiet environment without external light source, pure water or a zebra fish stock culture solution with the liquid level 10 mm-25 mm higher than the bottom is added into the container body 20, and the liquid between the light chamber 20a and the dark chamber 20b can be communicated with each other, so as to keep the liquid levels of the two chambers at the same level. The utility model relates to a phototaxis detection device's technical scheme can divide into following three stage in the test and detection process.

In the first stage of the testing process, referring to fig. 1, the test lamp 40 is fixed on the support member 10, the support member 10 is adjusted to make the test lamp 40 located 150mm above the container body 20, the partition member 30, the first cover plate 201 and the second cover plate 202 which can horizontally slide are kept in a closed state, a proper amount of zebra fish larvae which have good growth states and can autonomously swim to be tested are placed in a dark room 20b containing a certain volume of pure water or a zebra fish larvae stock solution, and the test lamp 40 is kept in a closed state. This phase is maintained for ten minutes to allow the zebrafish larvae to adapt to dark environmental conditions.

In the second stage of the testing process, referring to fig. 2 to 4, the testing lamp 40 is turned on to emit white light with approximately natural light, the first cover plate 201 horizontally slidable over the darkroom 20a is slid, the partition 30 is slid, and the second cover plate 202 horizontally slidable over the darkroom 20b is kept stationary, so that the darkroom 20a is kept under the illumination condition. This phase is maintained for five minutes to allow the zebrafish larvae to freely move about between the light chamber 20a and the dark chamber 20b.

And in the third stage of the test detection process, referring to fig. 5, the light chamber 20a and the dark chamber 20b are separated again by the sliding closed partition 30, the number of the zebra fish juvenile fishes in the light chamber 20a and the dark chamber 20b is counted, the value of the juvenile fishes in the light chamber 20a is divided by the total number of the zebra fish at the beginning of the test, normalization processing is performed, and in order to avoid accidental errors, three parallel tests should be repeated to obtain stable and accurate test results to evaluate the natural light tropism level of the zebra fish juvenile fishes.

Under the condition that the test conditions are kept unchanged, the light color of the test lamp 40 is changed, so that the light chamber 20a is respectively positioned under the illumination conditions of white light, red light, green light and blue light, the zebra fish juvenile fish with impaired eyesight of specific color can be screened out, the tropism data of the zebra fish juvenile fish to different color light is obtained, the visual sensitivity degree of the zebra fish to different color light is evaluated, the behavior of vision mediation can be used for rapidly screening out chemical substances which can damage the visual system of the zebra fish juvenile fish to specific color light from the viewpoint of scientific research, and good individuals of the visual system of the juvenile fish can be selected out from the viewpoint of agricultural cultivation for breeding, so that the variety optimization of fish is promoted.

It is required to explain, the utility model discloses a dark and natural white light transform, the zebra fish juvenile fish can receive natural light's stimulation under the simulation natural condition, through the change of zebra fish juvenile fish quantity in two cavities, the phototaxis reaction of analysis zebra fish. Through the conversion between darkness and natural white light, zebra fish juvenile fishes with imperfect visual functions or damaged visual functions can be continuously kept in a dark environment, so that individuals with zebra fish visual defects can be selected, and a sample is provided for further researching the causes of the visual defects; and the zebra fish juvenile fish with a good visual system can move from a dark environment to an illuminated environment in the direction of illumination, so that individuals with normal visual development of the zebra fish can be selected, and optimization of zebra fish varieties is facilitated. Through changing the light color of the test lamp 40, the zebra fish juvenile fish can be stimulated by light with a specific color, and phototaxis data of the zebra fish to the light with the specific color can be obtained so as to research the inducement of the zebra fish juvenile fish to the visual defect of the light with the specific color. Through the light color that changes test lamp 40, the zebra fish juvenile fish can receive the stimulation of different colours light, compares the phototaxis data who obtains, and the vision sensitivity degree of analysis zebra fish juvenile fish to different light.

In order to improve the scalability and convenience of screening the zebra fish juvenile fish having the visual defect, referring to fig. 1, in one embodiment, the length of the container body 20 may be 90 to 110mm, and may preferably be 104mm; the width of the container body 20 may be 20 to 40mm, and may preferably be 30mm; the height of the container body 20 may be 30 to 50mm, and may preferably be 40mm, without limitation thereto.

Further, the box 200 may be formed by five black opaque acrylic plates at the front, back, left, right, and bottom, where the height of the front and back plates may be 26mm, the height of the left and right plates may be 40mm, and the thickness of the bottom plate may be 5mm, but not limited thereto.

Referring to fig. 1 and 5, the box body 200 may be provided with grooves in a vertical direction between the inner walls of the front and rear plates and in a horizontal direction at the top of the left and right plates to form a first chute 20c and a second chute 20d, and the grooves may have a width of 4.8mm and a depth of 2mm, but not limited thereto.

Further, the first cover plate 201 and the second cover plate 202 may be of the same specification, and may have a length of 50mm, a width of 24mm, and a height of 4.5mm, without limitation.

Preferably, the top plane of the first cover plate 201 and the second cover plate 202 in the closed state may be disposed slightly lower than the top plane of the partition 30, so as to facilitate the partition 30 to be freely opened or closed.

Further, the partition 30 may also be provided in a plate shape; the length of the spacer 30 is 20 to 30mm, preferably 24mm; the width of the spacer 30 is 4 to 5mm, and preferably 4.5mm; the height of the spacer 30 is 30 to 40mm, and preferably 35mm, but not limited thereto.

The above is only the optional embodiment of the present invention, and not the scope of the present invention is limited thereby, all the equivalent structure changes made by the contents of the specification and the drawings are utilized under the inventive concept of the present invention, or the direct/indirect application in other related technical fields is included in the patent protection scope of the present invention.

Claims (10)

1. A phototaxis detection device, comprising:

a support member;

the container body is arranged on the support part and used for accommodating an animal to be tested;

a partition switchably provided on the container body in an open position and a closed position, the partition dividing an inside of the container body into a light chamber and a dark chamber;

in the open position, the light chamber is in communication with the dark chamber;

in the closed position, the light chamber is not in communication with the dark chamber; and

the test lamp, install in on the support piece, just the light-emitting side of test lamp is relative the light room sets up, the test lamp is used for transmitting light extremely in the light room.

2. The phototaxis detection device of claim 1, wherein the container body comprises a box body having a box opening, and a first cover plate and a second cover plate covering the box opening, the first cover plate forming an upper chamber wall of the light chamber, and the second cover plate forming an upper chamber wall of the dark chamber.

3. The phototaxis detection device of claim 2, wherein the first cover is movably disposed on the box along the length of the container; and/or

The second cover plate is movably arranged on the box body along the length direction of the container body.

4. The phototaxis detection device according to claim 3, wherein a first slide groove extending in a longitudinal direction of the inner wall of the box body is formed at a position near the box opening, and both sides of the first cover and the second cover are slidably disposed in the first slide groove, respectively.

5. The phototaxis detection device of claim 3 wherein the spacer is movably disposed in the middle of the box body in the height direction of the container body so that the spacer is switchably disposed on the container body in the open position and the closed position.

6. The apparatus according to claim 5, wherein the box body has a second slot extending along the height direction of the box body, and the two sides of the partition are slidably disposed in the second slot.

7. The phototaxis detection device according to claim 1, wherein the test lamp is movably disposed on the support member in a height direction of the container body.

8. The phototaxis detection device of any one of claims 1 to 7, wherein the container body is made of black opaque acrylic; and/or

The length of the container body is 90-110 mm, the width of the container body is 20-40 mm, and the height of the container body is 30-50 mm.

9. The phototaxis detection device of claim 8 wherein the spacer is plate-shaped; the length of the isolation piece is 20-30 mm, the width of the isolation piece is 4-5 mm, and the height of the isolation piece is 30-40 mm.

10. The phototaxis detection device of any one of claims 1 to 7, wherein the test lamp is an LED lamp.

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