CN217566433U

CN217566433U - Mouse cerebrospinal fluid collecting and supplementing device

Info

Publication number: CN217566433U
Application number: CN202220137223.2U
Authority: CN
Inventors: 吴洪玉; 方雪; 贺子轩; 宋铱航; 康乐; 吴佳艺
Original assignee: First Affiliated Hospital of Naval Military Medical University of PLA
Current assignee: First Affiliated Hospital of Naval Military Medical University of PLA
Priority date: 2022-01-18
Filing date: 2022-01-18
Publication date: 2022-10-14
Anticipated expiration: 2032-01-18

Abstract

The utility model discloses a mouse cerebrospinal fluid collecting and supplementing device, which comprises a device main body, a selection sleeve and a puncture needle; the selection sleeve is rotatably arranged in the device main body; a three-way cavity is arranged inside the selection sleeve; the three-way cavity comprises a collection cavity, a liquid supplementing cavity and a closed cavity; the puncture needle is arranged on the device main body; the puncture needle is selectively communicated with the collection cavity and the fluid infusion cavity. The utility model discloses an above-mentioned device's cooperation is used, can accomplish the collection to mouse cerebrospinal fluid fast to can be fast to the mouse fluid infusion after gathering the completion, need not to recycle the pjncture needle and puncture, effectively reduce the injury to the mouse, and it is more convenient to operate.

Description

Mouse cerebrospinal fluid collecting and supplementing device

Technical Field

The utility model relates to an experimental device field especially relates to a mouse cerebrospinal fluid is gathered and fluid infusion device.

Background

Cerebrospinal fluid is a colorless and transparent liquid existing in the ventricles of brain and subarachnoid space, and its physiological or chemical indexes or expression of related target molecules indicate the life activities and health conditions of organisms. Therefore, in the neurobiology research using a mouse as an object, the cerebrospinal fluid is often required to be repeatedly extracted for many times under the condition of a living body, and the detection of relevant indexes is carried out, wherein the main collection method is to directly puncture and extract from the macropore of the occiput of the mouse through a glass capillary.

However, the following difficulties exist in the actual operation process:

1. because the ventricles of the brain and the subarachnoid space of the mouse are narrow and the blood vessels in the mouse are rich, the blood vessels are very easy to be damaged to cause bleeding, and the pollution of cerebrospinal fluid is caused;

2. the glass capillary is too thin, so that sufficient cerebrospinal fluid is difficult to accurately control and obtain, and the collection is difficult in the later period;

3. after the cerebrospinal fluid is collected, fluid supplementation (supplementing physiological saline with the same volume to maintain normal intracranial pressure) is needed, however, the requirement cannot be met simultaneously in all the current collecting devices, the fluid supplementation operation is complex afterwards, and secondary trauma and infection can be caused.

Therefore, a mouse cerebrospinal fluid collecting and replenishing device is required to solve the above problems.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a mouse cerebrospinal fluid is gathered and fluid infusion device to when realizing conveniently obtaining the detection sample, can also alleviate the injury to the mouse.

In order to solve the technical problem, the utility model provides a mouse cerebrospinal fluid collecting and supplementing device, which comprises a device main body, a selection sleeve and a puncture needle; the selection sleeve is rotatably arranged in the device main body; a three-way cavity is arranged inside the selection sleeve; the three-way cavity comprises a collection cavity, a liquid supplementing cavity and a closed cavity; the puncture needle is arranged on the device main body; the puncture needle is selectively communicated with the collection cavity and the fluid infusion cavity.

Further, the collection chamber is a vacuum chamber.

Furthermore, the collection cavity and the fluid infusion cavity are both provided with through holes matched with the puncture needle.

Furthermore, a driving assembly is arranged on the device main body and used for driving the selection sleeve to rotate; and the selection sleeve is provided with a limiting assembly for conveniently controlling the driving assembly to rotate at equal intervals.

Further, the driving assembly comprises a power supply assembly, a micro motor and a control button; the power supply assembly and the micro motor are both arranged inside the device main body; the output end of the micro motor is connected with the end part of the selection sleeve; the power supply assembly is electrically connected with the micro motor; the control button is arranged on the outer wall of the device main body and used for transmitting power to the micro motor.

Furthermore, the limiting assembly comprises an elastic connecting piece and a plurality of limiting holes; the elastic connecting piece is matched with the limiting hole; the elastic connecting piece is arranged on the outer wall of the selection sleeve; the plurality of limiting holes are annularly arranged on the inner wall of the device main body by taking the rotating center of the selection sleeve as a circle center.

Furthermore, the number of the limiting holes is three.

Furthermore, an electric push rod for pushing balance liquid is arranged in the liquid supplementing cavity.

Furthermore, the outer wall of the device main body is provided with a push button, and the push button is wirelessly connected with the electric push rod.

Further, a protective cover for protecting the puncture needle is sleeved on the end portion of the device main body.

Compared with the prior art, the utility model discloses following beneficial effect has at least:

by selectively communicating the puncture needle with the three-way chamber, when the puncture needle is not needed to be used, the puncture needle is connected with the closed cavity, and the whole device is in a closed state; when the collection device is used, the puncture needle and the collection cavity can be communicated for collection, then the fluid infusion cavity is rotated to be communicated with the puncture needle, fluid infusion is carried out, the puncture needle is not required to be repeatedly utilized for puncture, the damage to a mouse is effectively reduced, and the extraction is convenient.

Drawings

FIG. 1 is a schematic structural view of a mouse cerebrospinal fluid collecting and fluid supplementing device of the present invention;

FIG. 2 is a cross-sectional view of a position limiting component of the mouse cerebrospinal fluid collecting and fluid supplementing device of the present invention;

fig. 3 is a top cross-sectional view of the driving assembly of the mouse cerebrospinal fluid collecting and fluid supplementing device of the present invention.

Detailed Description

The mouse cerebrospinal fluid collection and fluid replacement device of the present invention will now be described in greater detail with reference to the drawings, in which preferred embodiments of the present invention are shown, it being understood that those skilled in the art may modify the invention described herein while still achieving the beneficial effects of the present invention. Accordingly, the following description should be construed as broadly as possible to those skilled in the art and not as limiting the invention.

The invention is described in more detail in the following paragraphs by way of example with reference to the accompanying drawings. The advantages and features of the present invention will become more fully apparent from the following description and appended claims. It should be noted that the drawings are in simplified form and are not to precise scale, and are provided for convenience and clarity in order to facilitate the description of the embodiments of the present invention.

As shown in fig. 1, the embodiment of the present invention provides a mouse cerebrospinal fluid collecting and fluid supplementing device, which comprises a device main body 1, a selection sleeve 2 and a puncture needle 4.

The selection sleeve 2 is rotatably mounted inside the device main body 1, a three-way chamber 3 is arranged inside the selection sleeve 2, and specifically, the three-way chamber 3 comprises a collection chamber 31, a fluid infusion chamber 32 and a closed chamber 33; the collection cavity 31 and the fluid infusion cavity 32 are respectively used for collecting and infusing mouse spinal fluid.

The puncture needle 4 is arranged on the device main body 1, the puncture needle 4 is selectively communicated with the collection cavity 31 and the fluid infusion cavity 32, and the puncture needle 4 is communicated with different cavities in a matching way, so that the aims of collecting mouse spinal fluid and infusing the mouse spinal fluid are achieved respectively.

In this embodiment, it should be noted that the puncture needle 4 is just attached to one end of the three-way chamber 3, so that the puncture needle 4 can be normally communicated with the collection chamber 31 or the fluid infusion chamber 32.

By rotating the selection sleeve 2, the collection chamber 31 or the fluid infusion chamber 32 can be selectively communicated with the puncture needle 4.

In addition, because the same puncture needle 4 is used for collecting and supplementing liquid, the puncture needle 4 is not needed to be used for puncture for multiple times, the damage to the mouse is effectively reduced, and the infection probability of the mouse caused by multiple punctures can be reduced.

A protective cover 9 for protecting the puncture needle 4 is fitted over an end portion of the apparatus main body 1.

In the embodiment, when in use, the puncture needle 4 is firstly communicated with the collection cavity 31 to extract the spinal fluid of the mouse, and after the extraction is finished, the puncture needle 4 is communicated with the fluid infusion cavity 32 to infuse the mouse fluid, so as to ensure the health of the mouse; after use, the puncture needle 4 is attached to the closed cavity 33, so that the whole device is in a closed state.

In order to facilitate the collection of the spinal fluid of the mouse by the collection cavity 31, the collection cavity 31 is a vacuum cavity, and the spinal fluid of the mouse is collected into the collection cavity 31 by using the characteristic of negative pressure adsorption.

In order to facilitate the selective communication between the collection cavity 31 and the puncture needle 4, through holes 5 matched with the puncture needle 4 are formed in both the collection cavity 31 and the fluid infusion cavity 32. Furthermore, a rubber gasket is arranged on the inner wall of the through hole 5, so that the mouse spinal fluid collected in the collection cavity 31 and the balance fluid in the fluid infusion cavity 32 cannot overflow when the balance fluid is not acted by external force.

Preferably, the rubber gasket is arranged in a circular convex shape, when the puncture needle 4 is communicated with the collection cavity 31 or the fluid infusion cavity 32, the convex part of the rubber gasket just surrounds the outer wall of the puncture needle 4, so that the sealing effect is better when the puncture needle 4 is communicated with the collection cavity 31 or the fluid infusion cavity 32.

In order to control the rotation of the selection sleeve 2 conveniently, a driving assembly 6 is arranged on the device main body 1 and used for driving the selection sleeve 2 to rotate.

Referring to fig. 3, in particular, the driving assembly 6 includes a power supply assembly 61, a micro motor 62 and a control button 63.

The power supply assembly 61 and the micro motor 62 are both arranged inside the device body 1, the output end of the micro motor 62 is connected with the end part of the selection sleeve 2, the power supply assembly 61 is electrically connected with the micro motor 62, and the control button 63 is arranged on the outer wall of the device body 1 and used for transmitting power to the micro motor 62. Wherein, the power source assembly 61 can be a miniature button cell.

By starting the control button 63, the power supply module 61 is enabled to supply power to the micro motor 62, and then the micro motor 62 drives the selection sleeve 2 to rotate (i.e. the puncture needle 4 is communicated with different chambers), so as to complete the rapid switching of the chambers communicated with the puncture needle 4.

Furthermore, in order to ensure that the puncture needle 4 can be quickly communicated with the chamber required by the user after the rotation of the selection sleeve 2 is completed each time, a limiting assembly 7 is arranged on the selection sleeve 2 and used for conveniently controlling the driving assembly 6 to rotate at equal intervals. By rotating the drive assembly 6 equidistantly (i.e. by controlling the angle of a single rotation of the selection sleeve 2), the puncture needle 4 can be quickly connected to the chamber required by the user.

Referring to fig. 2, in detail, the limiting assembly 7 includes an elastic connecting member 71 and a plurality of limiting holes 72; the elastic connecting piece 71 is matched with the limiting hole 72; the elastic connecting piece 71 is arranged on the outer wall of the selection sleeve 2; the plurality of limiting holes 72 are annularly arranged on the inner wall of the device main body 1 with the rotation center of the selection sleeve 2 as a circle center.

The blocking feeling caused by the clamping connection between the elastic connecting piece 71 and the limiting hole 72 is used for judging, namely when the control button 63 is triggered, when the selection sleeve 2 rotates to a certain position, the corresponding blocking feeling appears, if the rotation is stopped, the puncture needle 4 is communicated with the next cavity, and the control button 63 can be released.

Because the spacing holes 72 are arranged at equal intervals, the purpose of conveniently enabling the driving assembly 6 to rotate at equal intervals is achieved, wherein the elastic connecting piece 71 can adopt a buckling piece (such as an umbrella bead in an umbrella and in an arc-shaped structure) in the prior art, when the elastic connecting piece 71 is clamped with the spacing holes 72, the selection sleeve 2 can temporarily stop rotating or rotate very slowly, and when the force is continuously applied, the selection sleeve 2 can also continue to rotate. When the next chamber is needed, the control button 63 is continuously pressed, the elastic connecting piece 71 and the limiting hole 72 are separated from the matching state under the action of the driving force, and the elastic connecting piece 71 is contacted with the next limiting hole 72 and presents a blocking feeling.

In this embodiment, the three-way chambers 3 are disposed inside the selection sleeve 2, so that the three limiting holes 72 are disposed (can be adaptively adjusted according to actual requirements) so as to correspond to the three-way chambers 3 one to one.

In order to conveniently complete liquid supplementation for the mouse, an electric push rod 8 for pushing balance liquid is arranged inside the liquid supplementation cavity 32, a push button 81 is arranged on the outer wall of the device main body 1, and the push button 81 is in wired or wireless connection with the electric push rod 8. When the connection is a wired connection, the connection line can be mainly arranged in the wall of the main body 1, one end of the connection line, which is far away from the puncture needle 4, is connected with the electric push rod 8, and a certain length is reserved so as to meet the requirement of at least one circle of rotation; when the connection is wireless, a wireless transmitting end and a wireless receiving end can be respectively arranged on the push button 81 and the electric push rod 8. By pressing the pushing button 81, the electric push rod 8 is controlled to extend and retract, thereby completing the pushing of the balance liquid.

In addition, the puncture needle 4 is communicated with the liquid supplementing cavity 32, the puncture needle 4 is contacted with the balance liquid storage bottle, and the electric push rod 8 reversely contracts, so that the balance liquid extraction function can be completed.

To sum up, the utility model discloses a concrete operating procedure as follows:

before use, the protective cover 9 is pulled out of the super clean bench, the control button 63 is pressed to enable the fluid infusion cavity 32 to be communicated with the puncture needle 4, the electric push rod 8 reversely contracts to complete extraction of balance fluid, then the balance fluid rotates to the position of the closed cavity 33, at the moment, the whole device is in a closed state, and the protective cover 9 is covered.

After the mouse is anesthetized, the head of the mouse is fixed and punctured by the puncture needle 4, the position of the device is kept still, the forefinger of the right hand immediately presses the control button 63 to the collection cavity 31, and cerebrospinal fluid is pumped back to the collection cavity 31 under the negative pressure action of the collection cavity 31.

After the collection is finished, the control button 63 is pressed to enable the puncture needle 4 to be communicated with the fluid infusion cavity 32, the push button 81 is pressed by the thumb, and the balance fluid is slowly pushed into the cerebrospinal cavity through the electric push rod 8 so as to maintain the pressure in the cerebrospinal cavity.

After the fluid infusion is finished, the outer muscle and the skin of the mouse are sutured, and penicillin powder is scattered to prevent infection.

The utility model discloses need not to utilize pjncture needle 4 to puncture the mouse and accomplish respectively and gather and the fluid infusion many times, effectively reduce the injury to the mouse, and can also effectively reduce operating personnel's the operation degree of difficulty (accomplish promptly after the puncture, only need can be present through pressing down control button 63 and push button 81 and gather the function with the fluid infusion), and can also one-hand operation, it is comparatively convenient that the operation gets up.

It will be apparent to those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims and their equivalents, the present invention is also intended to include such modifications and variations.

Claims

1. A mouse cerebrospinal fluid collecting and supplementing device is characterized by comprising a device main body, a selection sleeve and a puncture needle;

the selection sleeve is rotatably arranged in the device main body;

a three-way cavity is arranged inside the selection sleeve;

the three-way cavity comprises a collection cavity, a liquid supplementing cavity and a closed cavity;

the puncture needle is arranged on the device main body;

the puncture needle is selectively communicated with the collection cavity and the fluid infusion cavity.

2. The mouse cerebrospinal fluid collection and fluid replacement device of claim 1, wherein the collection chamber is a vacuum chamber.

3. The mouse cerebrospinal fluid collecting and fluid supplementing device according to claim 1, wherein the collecting chamber and the fluid supplementing chamber are both provided with through holes matched with the puncture needles.

4. The mouse cerebrospinal fluid collecting and fluid supplementing device according to claim 3, wherein a driving assembly is disposed on the device body for driving the selection sleeve to rotate;

and the selection sleeve is provided with a limiting assembly for conveniently controlling the driving assembly to rotate at equal intervals.

5. The mouse cerebrospinal fluid collection and fluid replacement device of claim 3, wherein the drive assembly comprises a power supply assembly, a micro-motor, and a control button;

the power supply assembly and the micro motor are both arranged inside the device main body;

the output end of the micro motor is connected with the end part of the selection sleeve;

the power supply assembly is electrically connected with the micro motor;

the control button is arranged on the outer wall of the device main body and used for transmitting power to the micro motor.

6. The mouse cerebrospinal fluid collecting and fluid supplementing device of claim 3, wherein the limiting component comprises an elastic connector and a plurality of limiting holes;

the elastic connecting piece is matched with the limiting hole;

the elastic connecting piece is arranged on the outer wall of the selection sleeve;

the plurality of limiting holes are annularly arranged on the inner wall of the device main body by taking the rotating center of the selection sleeve as a circle center.

7. The mouse cerebrospinal fluid collecting and fluid supplementing device of claim 6, wherein the number of the limiting holes is three.

8. The mouse cerebrospinal fluid collecting and fluid supplementing device according to claim 1, wherein an electric push rod for pushing the balancing fluid is disposed inside the fluid supplementing cavity.

9. The mouse cerebrospinal fluid collecting and fluid supplementing device according to claim 8, wherein a push button is disposed on an outer wall of the device body, and the push button is wirelessly connected to the electric push rod.

10. The mouse cerebrospinal fluid collecting and supplementing device of claim 1, wherein a protective cap for protecting the puncture needle is sleeved on an end of the device body.