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CN217230768U - Biological cell culture device - Google Patents

Biological cell culture device Download PDF

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Publication number
CN217230768U
CN217230768U CN202220065870.7U CN202220065870U CN217230768U CN 217230768 U CN217230768 U CN 217230768U CN 202220065870 U CN202220065870 U CN 202220065870U CN 217230768 U CN217230768 U CN 217230768U
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CN
China
Prior art keywords
incubator
spring
cell culture
biological cell
bottle
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CN202220065870.7U
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Chinese (zh)
Inventor
赖新鑫
王影
高舒平
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Zhang Chen
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Zhengzhou University of Industrial Technology
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Priority to CN202220065870.7U priority Critical patent/CN217230768U/en
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Abstract

The utility model discloses a biological cell culture device, belonging to the technical field of biological cell culture, comprising an incubator, a culture bottle, a placing seat arranged on the inner bottom surface of the incubator and a box door hinged on the front side surface of the incubator, wherein the upper surface of the placing seat is provided with three placing grooves, and the culture bottle is placed in the placing grooves; this biological cell culture device, through setting up locating lever and spring two, the effect of standing groove on the seat is placed in the utilization, place the blake bottle, cooperation locating lever and constant head tank matched with effect, when making the blake bottle get into the standing groove and extrude the locating lever, the locating lever receives the reaction force of spring one to fix the blake bottle in getting into the constant head tank fast, combine the effect of spring two and bottom plate, after the blake bottle is relieved fixedly, the bottom plate receives the reaction force of spring two to be fast with the ejecting standing groove of blake bottle, and then the staff alright carry out the work of taking to the blake bottle fast, time saving and labor saving.

Description

Biological cell culture device
Technical Field
The utility model relates to a biological cell culture technical field especially relates to a biological cell culture device.
Background
Cell culture is also called cell cloning technology, the formal term in biology is cell culture technology, and cell culture is an essential process for the whole bioengineering technology and one of the biotechnology, cell culture itself is large-scale cloning of cells, cell culture technology can be used for culturing a large amount of cells into simple single cells or cells with few differentiation, which is an essential link of cloning technology, and cell culture itself is cloning of cells, and a large amount of cells or metabolites thereof are obtained through cell culture, and biological products are obtained from the cells, so that the cell culture technology is the most core and basic technology in biotechnology.
Among the prior art, add the culture solution to the cell culture bottle, then put into culture apparatus with the blake bottle and carry out cell culture, but current blake bottle is when taking, because placer's height is higher, can not be fine take out the blake bottle from culture apparatus, and then waste time and energy.
SUMMERY OF THE UTILITY MODEL
The utility model provides a pair of biological cell culture device has solved prior art, to adding the culture solution in the cell culture bottle, then carries out cell culture in putting into culture apparatus with the blake bottle, but current blake bottle is when taking, because placer's height is higher, can not be fine take out the blake bottle from culture apparatus, and then the problem that wastes time and energy.
In order to realize the purpose, the utility model adopts the following technical scheme: the utility model provides a biological cell culture device, includes incubator, blake bottle, installs the seat of placing of bottom surface in the incubator and articulates the chamber door at the incubator leading flank, three standing groove has been seted up to the upper surface of placing the seat, the blake bottle is placed in the standing groove, the round hole has been seted up to the leading flank of placing the seat, be provided with the locating lever in the round hole, the one end of locating lever extends to in the constant head tank that the blake bottle leading flank was seted up, the baffle is installed to the other end of locating lever, spring one has been cup jointed on the locating lever, the both ends of spring one respectively with baffle and standing groove fixed connection, spring two is installed to the interior bottom surface of standing groove, the bottom plate is installed to the other end of spring two, the top and the bottom plate of blake bottle are inconsistent.
Adopt above-mentioned scheme, through setting up locating lever and spring two, the effect of standing groove on the seat is placed in the utilization, place the blake bottle, cooperation locating lever and constant head tank matched with effect, when making the blake bottle get into the standing groove and extrude the locating lever, the locating lever receives the reaction force of spring one to fix the blake bottle in getting into the constant head tank fast, combine the effect of spring two and bottom plate, after the blake bottle is relieved fixedly, the bottom plate receives the reaction force of spring two and fixes the ejecting standing groove of blake bottle fast, and then the staff alright carry out work labour saving and time saving to the blake bottle fast.
As a preferable embodiment, a telescopic rod is arranged in the second spring, the telescopic rod is installed on the inner bottom surface of the placing groove, and a telescopic shaft of the telescopic rod is fixedly connected with the bottom plate.
By adopting the scheme, through setting the telescopic rod, the extension of the telescopic rod is utilized to carry out limiting support on the second spring, so that the second spring can stably push the bottom plate.
As a preferred embodiment, the left and right sides of the inner wall of the placing groove are both provided with a clamping groove, a clamping plate is arranged in the clamping groove, and the clamping plate is arranged on the side surface of the bottom plate.
Adopt above-mentioned scheme, through setting up the cardboard, utilize the gliding effect of cardboard in the draw-in groove, carry out spacing support to the bottom plate when removing, stability when improving the bottom plate and removing, and then improve the stability of blake bottle.
As a preferred embodiment, a top plate is arranged above the culture bottle, a sealing plug is mounted on the lower surface of the top plate, one end of the sealing plug is located in the culture bottle and attached to the inner wall of the culture bottle, two bolts are mounted on the lower surface of the top plate, and one ends of the bolts extend into slots formed in the upper surface of the placing seat.
Adopt above-mentioned scheme, through setting up the roof, utilize the roof to fix the sealing plug, the effect of cooperation sealing plug is sealed the mouth of pipe department of blake bottle, and when avoiding taking the blake bottle, the culture solution in the blake bottle spills, and through the effect of bolt and slot, it is spacing to strengthen the roof.
In a preferred embodiment, the rear side of the incubator is provided with an alcohol tank and a liquid pump, the liquid inlet pipe of the liquid pump is communicated with the alcohol tank, the liquid outlet pipe of the liquid pump is communicated with a delivery pipe, and one end of the delivery pipe penetrates through the incubator and extends into the incubator and is provided with a spraying assembly.
Adopt above-mentioned scheme, through setting up the alcohol tank, utilize the alcohol of alcohol incasement to carry out the disinfection work of regularly disinfecting in to the incubator, the more bacterium of fostering in avoiding the incubator, cooperation liquid pump's effect is extracted the alcohol of alcohol incasement, and the cooperation sprays the effect of subassembly, evenly sprays alcohol work in to the incubator.
As a preferred embodiment, the spraying assembly comprises a connecting pipe arranged on the inner top surface of the incubator, one end of the conveying pipe is communicated with the connecting pipe, a plurality of spraying pipes are arranged on the lower surface of the connecting pipe, and two U-shaped ultraviolet lamp tubes are arranged on the inner top surface of the incubator.
Adopt above-mentioned scheme, through setting up the connecting pipe, utilize the effect that connecting pipe and conveyer pipe are linked together, carry the alcohol of alcohol incasement, carry out alcohol disinfection work in the incubator to the effect of cooperation shower nozzle, utilize ultraviolet tube to strengthen the disinfection in to the incubator, and then improve the aseptic nature in the incubator.
The utility model discloses in:
according to the biological cell culture device, the positioning rod and the second spring are arranged, the culture bottle is placed by utilizing the action of the placing groove on the placing seat, and the positioning rod is matched with the action of the positioning groove, so that when the culture bottle enters the placing groove to extrude the positioning rod, the positioning rod quickly enters the positioning groove to fix the culture bottle under the reaction force of the first spring, and the action of the second spring and the action of the bottom plate are combined, after the culture bottle is fixed, the bottom plate quickly pushes the culture bottle out of the placing groove under the reaction force of the second spring, and further, a worker can quickly take the culture bottle, so that time and labor are saved;
this biological cell culture device through setting up the alcohol tank, utilizes the alcohol of alcohol incasement to carry out the disinfection work of regularly disinfecting in to the incubator, and the more bacterium of breeding in avoiding the incubator, the effect of cooperation liquid pump is taken out the alcohol of alcohol incasement, and the cooperation sprays the effect of subassembly, evenly sprays alcohol work in to the incubator.
Drawings
Fig. 1 is a schematic structural view of the present invention;
fig. 2 is a schematic view of the top-view cross-sectional structure of the placing seat of the present invention;
fig. 3 is a schematic view of the front section structure of the placing seat of the present invention;
fig. 4 is a rear view structure diagram of the present invention;
fig. 5 is a schematic bottom view of the present invention.
In the figure: 1. an incubator; 2. a placing seat; 3. a culture bottle; 4. positioning a rod; 5. a baffle plate; 6. a first spring; 7. a second spring; 8. a base plate; 9. a telescopic rod; 10. clamping a plate; 11. a top plate; 12. a sealing plug; 13. an alcohol tank; 14. a liquid pump; 15. a delivery pipe; 16. an ultraviolet lamp tube; 17. and (4) connecting the pipes.
Detailed Description
Examples
Referring to fig. 1-5, the utility model provides a biological cell culture device, which comprises an incubator 1, a culture bottle 3, a placing seat 2 arranged on the inner bottom surface of the incubator 1 and a box door hinged on the front side surface of the incubator 1, wherein the incubator 1 is sealed by the box door, three placing grooves are arranged on the upper surface of the placing seat 2, the culture bottle 3 is placed by the placing grooves, the culture bottle 3 is placed in the placing grooves, a circular hole is arranged on the front side surface of the placing seat 2, a positioning rod 4 is arranged in the circular hole, one end of the positioning rod 4 extends into a positioning groove arranged on the front side surface of the culture bottle 3, the culture bottle 3 is fixed by the matching action of the positioning rod 4 and the positioning groove, a baffle 5 is arranged at the other end of the positioning rod 4, a spring 6 is sleeved on the positioning rod 4, the positioning rod 4 is assisted by the spring 6, the two ends of the spring 6 are respectively fixedly connected with the baffle 5 and the placing seat 2, spring two 7 is installed to the interior bottom surface of standing groove, utilizes the elasticity of spring two 7 to shift out culture bottle 3, and bottom plate 8 is installed to the other end of spring two 7, utilizes bottom plate 8 to support culture bottle 3, and the top of culture bottle 3 is inconsistent with bottom plate 8.
Be provided with telescopic link 9 in the second spring 7, utilize telescopic link 9 to carry out spacing support to second spring 7, telescopic link 9 is installed at the interior bottom surface of standing groove, telescopic link 9's telescopic shaft and bottom plate 8 fixed connection.
The draw-in groove has all been seted up to the left and right sides face of standing groove inner wall, utilizes the draw-in groove to place the one end of cardboard 10, is provided with cardboard 10 in the draw-in groove, cooperates the effect of cardboard 10 and draw-in groove, strengthens the support to bottom plate 8, and the side at bottom plate 8 is installed to cardboard 10.
The top of blake bottle 3 is provided with roof 11, the lower surface mounting of roof 11 has sealing plug 12, utilize sealing plug 12 to seal the mouth of pipe of blake bottle 3, the one end of sealing plug 12 is located blake bottle 3, and laminate mutually with the inner wall of blake bottle 3, the lower surface mounting of roof 11 has two bolts, the one end of bolt extends to in placing the slot that 2 upper surfaces of seat were seted up, utilize bolt and slot matched with effect, it is spacing to roof 11.
Alcohol tank 13 and liquid pump 14 are installed to the trailing flank of incubator 1, utilize the alcohol of 14 extraction alcohol tanks of liquid pump 13 interior to disinfect in the incubator 1, and the feed liquor pipe of liquid pump 14 is linked together with alcohol tank 13, and the drain pipe intercommunication of liquid pump 14 has conveyer pipe 15, utilizes conveyer pipe 15 to carry alcohol, and the one end of conveyer pipe 15 is run through and is extended to in the incubator 1 and be provided with the spray assembly.
The spraying assembly comprises a connecting pipe 17 arranged on the inner top surface of the incubator 1, one end of the conveying pipe 15 is communicated with the connecting pipe 17, a plurality of spraying pipes are arranged on the lower surface of the connecting pipe 17, the spraying pipes are used for spraying alcohol evenly in the incubator 1, and two U-shaped ultraviolet lamp tubes 16 are arranged on the inner top surface of the incubator 1.
The working principle is as follows: when the culture bottle 3 needs to be taken out, the box door is opened, then the baffle 5 moves outwards, the baffle 5 drives the positioning rod 4 to slide in the circular hole, the first spring 6 is stretched, one end of the positioning rod 4 is separated from the positioning groove, after the positioning rod 4 releases the fixation of the culture bottle 3, the soleplate 8 is subject to the reaction force of the second spring 7 to move the soleplate 8 upwards in the placing groove, the soleplate 8 drives the culture bottle 3 to move out of the placing groove, the clamping plate 10 is driven to move in the clamping groove when the soleplate 8 moves, the culture bottle 3 can be taken out from the placing groove, when the culture box 1 needs to be sterilized, the ultraviolet lamp tube 16 and the liquid pump 14 are started, the liquid inlet pipe of the liquid pump 14 pumps the alcohol in the alcohol tank 13, then the alcohol is conveyed into a liquid outlet pipe, and the alcohol is conveyed into a spraying pipe through the conveying pipe 15 and the connecting pipe 17 to carry out disinfection treatment on the culture box 1.

Claims (6)

1. The biological cell culture device is characterized by comprising an incubator (1), a culture bottle (3), a placing seat (2) installed on the inner bottom surface of the incubator (1) and a box door hinged to the front side surface of the incubator (1), wherein three placing grooves are formed in the upper surface of the placing seat (2), the culture bottle (3) is placed in the placing grooves, a circular hole is formed in the front side surface of the placing seat (2), a positioning rod (4) is arranged in the circular hole, one end of the positioning rod (4) extends into a positioning groove formed in the front side surface of the culture bottle (3), a baffle (5) is installed at the other end of the positioning rod (4), a first spring (6) is sleeved on the positioning rod (4), two ends of the first spring (6) are fixedly connected with the baffle (5) and the placing seat (2) respectively, a second spring (7) is installed on the inner bottom surface of each placing groove, and a bottom plate (8) is installed at the other end of the second spring (7), and the top end of the culture bottle (3) is abutted to the bottom plate (8).
2. The biological cell culture device according to claim 1, wherein a telescopic rod (9) is arranged in the second spring (7), the telescopic rod (9) is arranged on the inner bottom surface of the placing groove, and a telescopic shaft of the telescopic rod (9) is fixedly connected with the bottom plate (8).
3. The biological cell culture device according to claim 1, wherein the left and right sides of the inner wall of the placement groove are both provided with clamping grooves, clamping plates (10) are arranged in the clamping grooves, and the clamping plates (10) are arranged on the side surfaces of the bottom plate (8).
4. The biological cell culture device according to claim 1, wherein a top plate (11) is arranged above the culture bottle (3), a sealing plug (12) is arranged on the lower surface of the top plate (11), one end of the sealing plug (12) is positioned in the culture bottle (3) and is attached to the inner wall of the culture bottle (3), two bolts are arranged on the lower surface of the top plate (11), and one ends of the bolts extend into slots formed in the upper surface of the placing seat (2).
5. The biological cell culture device according to claim 1, wherein an alcohol tank (13) and a liquid pump (14) are installed on the rear side surface of the incubator (1), a liquid inlet pipe of the liquid pump (14) is communicated with the alcohol tank (13), a liquid outlet pipe of the liquid pump (14) is communicated with a delivery pipe (15), and one end of the delivery pipe (15) extends into the incubator (1) in a penetrating manner and is provided with a spraying assembly.
6. The biological cell culture apparatus according to claim 5, wherein the spraying assembly comprises a connecting pipe (17) installed on the inner top surface of the incubator (1), one end of the conveying pipe (15) is communicated with the connecting pipe (17), a plurality of spraying pipes are installed on the lower surface of the connecting pipe (17), and two U-shaped ultraviolet lamps (16) are installed on the inner top surface of the incubator (1).
CN202220065870.7U 2022-01-12 2022-01-12 Biological cell culture device Active CN217230768U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202220065870.7U CN217230768U (en) 2022-01-12 2022-01-12 Biological cell culture device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202220065870.7U CN217230768U (en) 2022-01-12 2022-01-12 Biological cell culture device

Publications (1)

Publication Number Publication Date
CN217230768U true CN217230768U (en) 2022-08-19

Family

ID=82830036

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202220065870.7U Active CN217230768U (en) 2022-01-12 2022-01-12 Biological cell culture device

Country Status (1)

Country Link
CN (1) CN217230768U (en)

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GR01 Patent grant
GR01 Patent grant
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TR01 Transfer of patent right

Effective date of registration: 20240527

Address after: 518000 room 110, No. 12, Xin'an Sixth Road, Xixiang, Bao'an District, Shenzhen, Guangdong Province

Patentee after: Zhang Chen

Country or region after: China

Address before: 450000 Xinzheng high tech Development Zone, Xinzheng City, Zhengzhou City, Henan Province

Patentee before: ZHENGZHOU University OF INDUSTRIAL TECHNOLOGY

Country or region before: China