CN216584999U - Automatic nucleic acid extraction apparatus - Google Patents

Abstract

The present invention relates to an automatic nucleic acid extraction apparatus. The automatic nucleic acid extraction apparatus includes: a mounting frame; the sample carrier is arranged in the mounting frame and is used for storing the sampling pipe; the clamping mechanism is arranged in the mounting frame and is provided with a clamping position for clamping or loosening the sampling tube; the nucleic acid extraction mechanism is arranged in the mounting frame and is used for extracting nucleic acid; the cover opening mechanism is arranged in the installation frame and is used for transferring the sampling pipe between the sample carrier and the clamping position and opening or closing the sampling pipe clamped at the clamping position; and a liquid transfer mechanism which is arranged in the installation frame and is used for sucking the liquid in the sampling pipe positioned at the clamping position and transferring the liquid to the nucleic acid extraction mechanism.

Description

Automatic nucleic acid extraction apparatus

Technical Field

The utility model relates to the technical field of biological detection, in particular to automatic nucleic acid extraction equipment.

Background

The nucleic acid detecting substance is nucleic acid of virus to determine whether the patient is infected by virus by searching whether nucleic acid of virus invaded from the outside exists in the respiratory tract specimen, blood or feces of the patient. Nucleic acid extraction equipment is required for detecting nucleic acid.

However, the existing nucleic acid extraction apparatus includes a plurality of relatively independent instruments such as a liquid separation instrument, a nucleic acid extraction instrument, and the like. For example, the fully automatic nucleic acid extractor disclosed in patent document No. CN213266452U has only a nucleic acid extracting function, but does not have a cup separating function, and needs to manually separate cups, which greatly increases the burden on personnel and labor intensity; for example, a new coronavirus sample dispensing device disclosed in patent document No. CN113150959A has only a dispensing function, and the process of nucleic acid extraction and transfer after dispensing is complicated, and the extract solution is easily contaminated. An automatic cup dispenser disclosed in patent document CN13244972A has no lid opening function, and can only open the lid manually, which is likely to pollute the extract, and increases the labor intensity of the worker, resulting in low automation.

SUMMERY OF THE UTILITY MODEL

Therefore, it is necessary to provide an automatic nucleic acid extraction device for improving the above defects, aiming at the problems that in the prior art, the nucleic acid extraction device comprises a plurality of instruments, an extracting solution needs to be transferred among the instruments, the pollution risk is increased, the process is complicated, the automation degree is low, and the labor intensity of workers is increased.

An automatic nucleic acid extraction apparatus comprising:

a mounting frame;

a sample carrier mounted within the mounting frame and used for storing a sample tube;

the clamping mechanism is arranged in the mounting frame and is provided with a clamping position for clamping or loosening the sampling tube;

a nucleic acid extraction mechanism installed in the mounting frame and configured to extract nucleic acid;

the cover opening mechanism is arranged in the mounting frame and is used for transferring the sampling tube between the sample carrier and the clamping position and opening or closing the sampling tube clamped at the clamping position; and

and the liquid transferring mechanism is arranged in the mounting frame and is used for sucking the liquid in the sampling pipe positioned at the clamping position and transferring the liquid to the nucleic acid extracting mechanism.

In one embodiment, the automatic nucleic acid extraction equipment further comprises a gun head carrier installed in the mounting frame, and the gun head carrier is used for storing gun heads;

the pipetting mechanism comprises a first driving component, a transferring seat in driving connection with the first driving component and a single-channel pipetting component arranged on the transferring seat; the first driving component is used for driving the transfer seat to move along a horizontal plane and pass through the gun head carrier, the clamping position and the nucleic acid extracting mechanism, and the single-channel liquid-transfering component is provided with a first mounting head for detachably mounting the gun head;

when the transfer seat moves to the gun head carrier, the first mounting head can pick up the gun heads on the gun head carrier; when the transfer seat moves to the clamping position, the single-channel liquid transfer assembly sucks liquid in the sampling tube through the gun head; when the transfer seat moves to the nucleic acid extraction mechanism, the single-channel liquid transfer assembly can spit the liquid in the gun head to the nucleic acid extraction mechanism.

In one embodiment, the automatic nucleic acid extraction apparatus further comprises a reagent carrier mounted within the mounting frame, the reagent carrier for storing a reagent tube;

the pipetting mechanism also comprises a multi-channel pipetting assembly arranged on the transfer seat, and the multi-channel pipetting assembly is provided with a plurality of second mounting heads for detachably mounting the gun heads;

the transfer seat is also passed through the reagent carrier under the driving action of the first driving assembly; when the transfer seat moves to the gun head carrier, the second mounting heads can simultaneously pick up the gun heads on the gun head carrier; when the transfer seat moves to the nucleic acid extraction mechanism, the multi-channel pipetting assembly can simultaneously suck the nucleic acid extracting solution of the nucleic acid extraction mechanism through a plurality of gun heads; when the transfer base moves to the reagent carrier, the multi-channel pipetting module can discharge the nucleic acid extracting solutions in the plurality of tips to the plurality of reagent tubes on the reagent carrier, respectively.

In one embodiment, the first driving assembly is used for driving the transfer seat to move along a first horizontal direction and/or a second horizontal direction intersecting with the first horizontal direction.

In one embodiment, the first driving assembly comprises a first cross beam movably connected to the mounting frame along the first horizontal direction, and the transfer base is movably connected to the first cross beam along the second horizontal direction.

In one embodiment, the first drive assembly further comprises a first driver, a first driving pulley, a first driven pulley, and a first drive belt;

the first driving part is mounted on the mounting frame, the first driving pulley is in transmission connection with an output shaft of the first driving part, the first driven pulley is rotatably connected on the mounting frame, and the first driving pulley and the first driven pulley are arranged at intervals along the first horizontal direction; the first transmission belt is sleeved between the first driving belt wheel and the first driven belt wheel and is connected with the first cross beam.

In one embodiment, the first driving assembly further includes a first lead screw, a second driving member, and a first lead screw nut, the first lead screw is rotatably connected to the first cross beam around its axis, and the axis of the first lead screw is parallel to the second horizontal direction, the second driving member is mounted on the first cross beam and is drivingly connected to the first lead screw, and the first lead screw nut is threadedly connected to the first lead screw and is fixedly connected to the transfer base.

In one embodiment, the pipetting mechanism further comprises a first lifting assembly and a second lifting assembly, the single-channel pipetting assembly is mounted on the transfer seat through the first lifting assembly, the first lifting assembly is used for driving the single-channel pipetting assembly to ascend or descend along the gravity direction, the multi-channel pipetting assembly is mounted on the transfer seat through the second lifting assembly, and the second lifting assembly is used for driving the multi-channel pipetting assembly to ascend or descend along the gravity direction.

In one embodiment, the first lifting assembly comprises a first lifting seat, a second screw, a first lifting driving member and a second screw nut; the first lifting seat is connected to the transfer seat in a lifting manner along the gravity direction, and the single-channel liquid transfer assembly is installed on the first lifting seat; the second screw rod is rotatably connected to the first lifting seat around the axis of the second screw rod, and the axis of the second screw rod is parallel to the gravity direction; the first lifting driving piece is arranged on the transfer seat and is in transmission connection with the second screw rod; and the second screw rod nut is in threaded connection with the second screw rod and is fixedly connected with the first lifting seat.

In one embodiment, the second lifting assembly comprises a second lifting seat, a third screw, a second lifting driving member and a third screw nut; the second lifting seat is connected to the transfer seat in a lifting manner along the gravity direction, and the multichannel pipetting assembly is installed on the second lifting seat; the third screw rod is rotatably connected to the second lifting seat around the axis of the third screw rod, and the axis of the third screw rod is parallel to the gravity direction; the second lifting driving piece is arranged on the transfer seat and is in transmission connection with the third screw rod; and the third screw rod nut is in threaded connection with the third screw rod and is fixedly connected with the second lifting seat.

In one embodiment, the nucleic acid extraction mechanism includes a plurality of nucleic acid extraction mechanisms capable of performing nucleic acid extraction independently of each other.

A nucleic acid extraction method using the automatic nucleic acid extraction apparatus as described in any one of the above embodiments, comprising the steps of:

the uncapping mechanism transfers the sampling tube on the sample carrier to the clamping position of the clamping mechanism;

the clamping mechanism clamps the sampling pipe positioned at the clamping position;

the tube cover on the sampling tube positioned at the clamping position is unscrewed by the cover opening mechanism;

the liquid transfer mechanism moves to the clamping position and sucks liquid in the sampling tube at the clamping position;

the pipetting mechanism moves to the nucleic acid extraction mechanism to release the aspirated liquid to the nucleic acid extraction mechanism;

the cover opening mechanism is used for screwing the tube cover on the sampling tube positioned at the clamping position again and transferring the sampling tube positioned at the clamping position to the sample carrier.

In practical use, the automatic nucleic acid extraction equipment and the nucleic acid extraction method thereof firstly place the sampling tube filled with a sample to be detected on the sample carrier. And the cover opening mechanism moves to the sample carrier and clamps the sampling pipe. Then, the cover opening mechanism moves to the clamping mechanism, and the clamped sampling pipe is placed at the clamping position of the clamping mechanism. The clamping mechanism clamps the sampling tube at the clamping position. At this moment, the tube cover of the sampling tube is unscrewed (namely, the tube cover is opened) by the tube opening mechanism, and the liquid transfer mechanism moves to the clamping position of the clamping mechanism and sucks the liquid in the sampling tube positioned at the clamping position. Then, the pipetting mechanism is moved to the nucleic acid extracting mechanism, and the aspirated liquid is released to the nucleic acid extracting mechanism. The nucleic acid extraction mechanism is used for extracting nucleic acid from the liquid. At the same time, the cap-opening mechanism screws the cap onto the sampling tube (and the cap) again. The clamping mechanism releases the sampling tube, and the uncapping mechanism transfers the sampling tube to the sample carrier again. And circulating in sequence according to the steps until the liquid in all the sampling pipes on the sample carrier is respectively transferred into the nucleic acid extracting mechanism, so that the nucleic acid extracting mechanism can respectively extract the nucleic acid of each sample.

Thus, the automatic nucleic acid extraction equipment integrates the clamping mechanism, the cover opening mechanism, the liquid transferring mechanism and the nucleic acid extraction mechanism, realizes the transfer of liquid in the sampling tube by utilizing the cover opening mechanism and the liquid transferring mechanism, and automatically unscrews and screws the tube cover of the sampling tube by utilizing the cover opening mechanism. Compared with the prior art, the automatic nucleic acid extraction equipment can automatically complete uncovering, cup separation and nucleic acid detection, sample solution does not need to be transferred among various instruments, and automatic transfer in the automatic nucleic acid extraction equipment is realized, so that the pollution risk is reduced, the steps are simplified, the automation degree is high, and the labor intensity of workers is favorably reduced.

Drawings

FIG. 1 is a perspective view of an automatic nucleic acid extracting apparatus according to an embodiment of the present invention;

FIG. 2 is a perspective view of the automatic nucleic acid extracting apparatus shown in FIG. 1 from another perspective;

FIG. 3 is a top view of the automatic nucleic acid extracting apparatus shown in FIG. 1;

FIG. 4 is a front view of the automatic nucleic acid extracting apparatus shown in FIG. 1;

FIG. 5 is a left side view of the automatic nucleic acid extracting apparatus shown in FIG. 1;

FIG. 6 is a right side view of the automatic nucleic acid extracting apparatus shown in FIG. 1;

FIG. 7 is a rear view of the automatic nucleic acid extracting apparatus shown in FIG. 1;

FIG. 8 is a flow chart of a method for extracting nucleic acid according to an embodiment of the present invention.

Detailed Description

In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in detail below. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein, as those skilled in the art will recognize without departing from the spirit and scope of the present invention.

In the description of the present invention, it is to be understood that the terms "central," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," "circumferential," and the like are used in the orientations and positional relationships indicated in the drawings for convenience in describing the utility model and to simplify the description, and are not intended to indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and are not to be considered limiting of the utility model.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or to implicitly indicate the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.

In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can, for example, be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.

In the present invention, unless otherwise expressly stated or limited, the first feature "on" or "under" the second feature may be directly contacting the first and second features or indirectly contacting the first and second features through an intermediate. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.

It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and the like as used herein are for illustrative purposes only and do not denote a unique embodiment.

Referring to fig. 1 to 3, an automatic nucleic acid extracting apparatus according to an embodiment of the present invention includes a mounting frame 10, a sample carrier 20, a clamping mechanism 30, a nucleic acid extracting mechanism 40, a cover opening mechanism 50, and a pipetting mechanism 60.

The sample carrier 20 is mounted within the mounting frame 10 and is used to store a sampling tube a 1. That is, the specimen to be tested is stored in the sampling tube a1 using a reagent such as a preservation solution. Of course, in order to improve the detection efficiency, the sample carrier 20 may store several sampling tubes a1 at the same time, so as to facilitate the subsequent nucleic acid extraction of the liquid in a plurality of sampling tubes a1 at the same time.

The clamping mechanism 30 is mounted in the mounting frame 10 and has a clamping position 31 for clamping or unclamping the sampling tube a 1. The nucleic acid extraction mechanism 40 is installed in the mounting frame 10 and is used for nucleic acid extraction of the liquid in the sampling tube a 1. The cover opening mechanism 50 is installed in the mounting frame 10, and is used for transferring the sampling tube a1 between the sample carrier 20 and the grip position 31 of the grip mechanism 30 and opening or closing the sampling tube a1 gripped at the grip position 31. The pipetting mechanism 60 is installed in the mounting frame 10 and is used for aspirating the liquid in the sampling tube a1 located at the holding position 31 and transferring to the nucleic acid extraction mechanism 40 so that the nucleic acid extraction mechanism 40 can extract the nucleic acid from the liquid.

In actual use, the automatic nucleic acid extracting apparatus described above first places the sampling tube a1 containing a specimen to be tested on the specimen carrier 20. The lid release mechanism 50 moves onto the sample carrier 20 and grips the sampling tube a 1. Then, the cap-opening mechanism 50 is moved to the gripping mechanism 30, and the gripped sampling tube a1 is placed in the gripping position 31 of the gripping mechanism 30. The gripping mechanism 30 grips the sampling tube a1 at the gripping location 31. At this time, the cap-opening mechanism 50 unscrews (i.e., opens) the cap of the sampling tube a1, and the pipette mechanism 60 moves to the grip position 31 of the grip mechanism 30 and sucks the liquid in the sampling tube a1 located at the grip position 31. Then, the pipetting mechanism 60 moves to the nucleic acid extracting mechanism 40, and the aspirated liquid is discharged to the nucleic acid extracting mechanism 40. The nucleic acid extraction mechanism 40 is used for extracting nucleic acid from the liquid. At the same time, the cap release mechanism 50 again screws the cap onto the sampling tube a1 (and the cap). The gripper mechanism 30 releases the sampling tube a1 and the decapper mechanism 50 transfers the sampling tube a1 to the sample carrier 20 again. The above steps are sequentially circulated until all the liquid in the sampling pipe a1 on the sample carrier 20 is respectively transferred to the nucleic acid extracting mechanism 40, so that the nucleic acid extracting mechanism 40 respectively extracts the nucleic acid from each sample.

Thus, the automatic nucleic acid extracting apparatus of the present invention integrates the gripping mechanism 30, the cap opening mechanism 50, the pipetting mechanism 60 and the nucleic acid extracting mechanism 40, and the transfer of the liquid in the sampling tube a1 is effected by the cap opening mechanism 50 and the pipetting mechanism 60, and the cap of the sampling tube a1 is automatically unscrewed and screwed by the cap opening mechanism 50. Compared with the prior art, the automatic nucleic acid extraction equipment can automatically complete uncovering, cup separation and nucleic acid detection, sample solution does not need to be transferred among various instruments, and automatic transfer in the automatic nucleic acid extraction equipment is realized, so that the pollution risk is reduced, the steps are simplified, the automation degree is high, and the labor intensity of workers is favorably reduced.

In particular, in the embodiment, the automatic nucleic acid extracting apparatus includes a plurality of nucleic acid extracting mechanisms 40, and the plurality of nucleic acid extracting mechanisms 40 are capable of performing nucleic acid extraction independently of each other. Thus, part or all of the nucleic acid extraction mechanisms 40 can be flexibly selected to respectively perform nucleic acid extraction operations according to specific conditions, which is beneficial to improving the nucleic acid extraction efficiency of equipment; on the other hand, the flexibility and the compatibility of the equipment in the actual use process are improved. In particular, in the embodiment shown in the drawings, the automatic nucleic acid detecting apparatus includes three nucleic acid extracting mechanisms 40, and the three nucleic acid extracting mechanisms 40 are arranged at intervals in the first horizontal direction described below so as to simplify the moving path of the liquid to be transferred to each nucleic acid extracting mechanism 40 by the pipetting mechanism 60.

Referring to FIGS. 4 to 7, in the embodiment of the present invention, the automatic nucleic acid extracting apparatus further comprises a gun head carrier 70 installed in the mounting frame 10, the gun head carrier 70 storing a gun head a 2. The pipetting mechanism 60 comprises a first drive assembly, a transfer receptacle 612 and a single channel pipetting assembly 62. The first driving component is drivingly connected to the transfer base 612 to drive the transfer base 612 to move along a horizontal plane and pass through the gun head carrier 70, the clamping position 31 of the clamping mechanism 30 and the nucleic acid extracting mechanism 40. The single pass pipetting assembly 62 has a first mounting head 621 for detachably mounting the tip a2 so that the single pass pipetting assembly 62 can aspirate or dispense liquid through the tip a2 mounted on the first mounting head 621.

When the transfer base 612 is moved to the tip carriage 70, the first mounting head 621 is able to pick up the tip a2 on the tip carriage 70. When the transfer base 612 moves to the holding position 31 of the holding mechanism 30, the single-channel pipetting module 62 can aspirate the liquid in the sampling tube a1 held in the holding position 31 through the tip a 2. When the transfer base 612 is moved to the nucleic acid isolation mechanism 40, the single-lane pipette module 62 can discharge the liquid in the tip a2 to the nucleic acid isolation mechanism 40 so that the nucleic acid isolation mechanism 40 can extract nucleic acid from the liquid. In this manner, in actual use, the carrier block 612 is first moved to the tip carriage 70 by the driving action of the first driving assembly, so that the first mounting head 621 of the single pass pipetting assembly 62 picks up the tips a2 on the tip carriage 70. Then, the transfer base 612 is moved to the holding position 31 of the holding mechanism 30, so that the single-channel pipetting assembly 62 sucks the liquid clamped in the sampling tube a1 of the holding position 31 through the gun head a 2. Then, the transfer base 612 is moved to the nucleic acid isolation mechanism 40 so that the single-path pipette unit 62 discharges the liquid to the nucleic acid isolation mechanism 40 through the tip a2 for the nucleic acid isolation mechanism 40 to extract the nucleic acid from the liquid.

In particular, in the embodiment, the automatic nucleic acid extracting apparatus further includes a reagent carrier 90 installed in the mounting frame 10, the reagent carrier 90 storing a reagent tube storing a reagent such as a preservation solution for receiving a nucleic acid extraction solution obtained after nucleic acid extraction by the nucleic acid extracting mechanism 40. The pipetting mechanism 60 further comprises a multi-channel pipetting assembly 63 mounted on the transfer block 612, the multi-channel pipetting assembly 63 having a plurality of second mounting heads 631 for detachably mounting the pipette tips a 2.

The carriage 612 is also routed through the reagent carrier 90 under the driving action of the first drive assembly. When the transfer base 612 is moved to the tip carrier 70, the plurality of second mounting heads 631 can simultaneously pick up the tips a2 on the tip carrier 70. When the transfer base 612 is moved to the nucleic acid isolation mechanism 40, the multi-channel pipetting module 63 can simultaneously aspirate the nucleic acid isolation solution from the nucleic acid isolation mechanism 40 through the plurality of pipette tips a2 picked up. When the transfer base 612 is moved to the reagent carriage 90, the multi-channel pipetting module 63 can discharge the nucleic acid extracting solutions in the plurality of tips a2 to the plurality of reagent tubes on the reagent carriage 90. Thus, in actual use, the nucleic acid extracting mechanism 40 completes nucleic acid detection and obtains a nucleic acid extract. The carriage 612 moves to the gun head carriage 70 so that the second mounting heads 631 of the multi-channel pipetting assembly 63 each pick up a gun head a 2. Then, the transfer base 612 is moved to the nucleic acid isolation mechanism 40, so that the multi-channel pipetting module 63 suctions the nucleic acid isolation solutions from the tips a2 on the multi-channel pipetting module. Then, the transfer base 612 is moved to the reagent carriage 90, so that the multi-channel pipetting module 63 discharges the nucleic acid extracting solution aspirated by each tip a2 thereon into the corresponding reagent tube.

It should be noted that the nucleic acid extracting solution from the nucleic acid extracting mechanism 40 is transferred to the reagent tube on the reagent carriage 90 by the multi-channel pipetting module 63, thereby improving the working efficiency of nucleic acid extraction and reducing the time cost. In addition, the automatic nucleic acid extraction equipment can flexibly select the single-channel pipetting component 62 and the multi-channel pipetting component 63 for pipetting operation, and is favorable for increasing the flexibility and the compatibility of the equipment.

In a specific embodiment, the automatic nucleic acid extracting apparatus further includes a waste pipette tip collecting mechanism 80 (see FIG. 3) installed in the mounting frame 10. The transfer block 612 is also routed through the waste lance head collection mechanism 80 under the driving action of the first drive assembly. When the transfer base 612 moves to the waste-tip collection mechanism 80, the single-path pipetting module 62 can release the tips a2 on the first mounting head 621 to the waste-tip collection mechanism 80, or the multi-path pipetting module 63 can release the tips a2 on the respective second mounting heads 631 to the waste-tip collection mechanism 80.

In this way, after the liquid in one sampling tube a1 is transferred to the nucleic acid extraction mechanism 40, the transfer base 612 is moved to the waste-tip collection mechanism 80, so that the single-channel pipetting module 62 releases the tip a2 of the first mounting head 621 to the waste-tip collection mechanism 80. The carriage 612 is then moved to the gun head carriage 70 so that the first mounting head 621 of the single pass pipetting assembly 62 picks up a new gun head a2 for the purpose of again transferring liquid from the other sampling tube a1 to avoid contamination. Similarly, after all the nucleic acid extraction liquid obtained by detecting nucleic acid in the liquid in the same sampling tube a1 is transferred to the reagent tube on the reagent carriage 90, the transfer base 612 is moved to the waste pipette tip collection mechanism 80, so that the multi-channel pipetting module 63 releases the pipette tips a2 on the respective second mounting heads 631 to the waste pipette tip collection mechanism 80. Then the transfer base 612 moves to the pipette head carrier 70 again, so that each second mounting head 631 of the multi-channel pipetting assembly 63 picks up a new pipette head a2, so as to transfer the nucleic acid extracting solution obtained after the liquid in another sampling tube a1 undergoes nucleic acid detection again, and avoid pollution.

In an embodiment of the present invention, the first driving assembly is configured to drive the transfer base 612 to move along a first horizontal direction and/or a second horizontal direction intersecting the first horizontal direction. In this way, the first driving assembly drives the transfer base 612 to move in the first horizontal direction and the second horizontal direction, so that the transfer base 612 can pass through the clamping position 31 of the clamping mechanism 30, the nucleic acid extracting mechanism 40, the reagent tube rack and the waste pipette tip collecting mechanism 80, and the single-channel pipetting assembly 62 and the multi-channel pipetting assembly 63 can respond. Preferably, the first horizontal direction is perpendicular to the second horizontal direction. Specifically, in the embodiment shown in fig. 3, the first horizontal direction is a left-right direction, the second horizontal direction is an up-down direction, and the gravity direction is a direction perpendicular to the paper surface.

It is understood that the transfer seat 612 is located above the clamping mechanism 30, the nucleic acid extracting mechanism 40, the reagent carrier 90 and the waste tip collecting mechanism 80. When the transfer block 612 is moved in the first horizontal direction and the second horizontal direction to above the holding position 31 of the holding mechanism 30, the single pass pipetting module 62 can insert the pipette tip a2 on the first mounting head 621 into the sampling tube a1 on the holding position 31 and aspirate the liquid in the sampling tube a 1. Similarly, when the transfer base 612 moves to the upper side of the pipette tip carrier 70, the nucleic acid extracting mechanism 40, the reagent carrier 90 or the waste pipette tip collecting mechanism 80 along the first horizontal direction and the second horizontal direction, the single-channel pipetting module 62 or the multi-channel pipetting module 63 can perform corresponding actions, which is not described in detail herein.

In some embodiments, the first drive assembly comprises a first cross member 611, the first cross member 611 being movably connected to the mounting frame 10 in a first horizontal direction. The transfer base 612 is movably connected to the first beam 611 along the second horizontal direction, so that the transfer base 612 can move along the first horizontal direction and the second horizontal direction.

In particular, in the embodiment, the first driving assembly further includes a first driving element 613, a first driving pulley 614, a first driven pulley 615 and a first transmission belt 616. The first driving element 613 is mounted on the mounting frame 10, and the first driving pulley 614 is drivingly connected to an output shaft of the first driving element 613, so that the first driving element 613 can drive the first driving pulley 614 to rotate. The first driven pulley 615 is rotatably connected to the mounting frame 10, and the first driving pulley 614 and the first driven pulley 615 are arranged at an interval in the first horizontal direction. The first driving belt 616 is sleeved between the first driving pulley 614 and the first driven pulley 615, and is connected to the first beam 611. In this way, when the transfer seat 612 needs to be driven to move along the first horizontal direction, the first driving element 613 drives the first driving pulley 614 to rotate, so as to drive the first driving belt 616 to sequentially move forward between the first driving pulley 614 and the first driven pulley 615, and further drive the first beam 611 and the transfer seat 612 on the first beam 611 to move along the first horizontal direction. Alternatively, the first drive 613 may be a motor. The first driving pulley 614 and the first driven pulley 615 may employ a timing pulley, and the first driving belt 616 may employ a timing belt.

It should be noted that the first driving assembly is not limited to use a belt transmission structure to realize the movement of the transfer seat 612 along the first horizontal direction, and in other embodiments, the first driving assembly may also use a rack-and-pinion transmission structure or a lead screw pair transmission structure, and is not limited herein.

Specifically, in the embodiment, the first driving assembly further includes a first lead screw, a second driving member 617 and a first lead screw nut. The first lead screw is rotatably connected to the first beam 611 around its axis, and the axis of the first lead screw is parallel to the second horizontal direction. The second driving part 617 is mounted on the first cross beam 611 and is in driving connection with the first lead screw to drive the first lead screw to rotate around its axis. The first lead screw nut is connected to the first lead screw in a threaded manner and is fixedly connected with the transfer base 612. Thus, when the transfer seat 612 needs to be driven to move along the second horizontal direction, the second driving part 617 drives the first lead screw to rotate around its axis, so as to drive the first lead screw nut to move along the axial direction of the first lead screw (i.e. the second horizontal direction), and the first lead screw nut drives the transfer seat 612 to move along the second horizontal direction. Alternatively, the second driving member 617 may employ a motor.

It should be noted that the first driving assembly is not limited to use the above-mentioned screw assembly transmission structure to drive the transfer base 612 to move along the second horizontal direction. In other embodiments, a rack and pinion transmission structure, a belt transmission structure, etc. may be used, and are not limited herein.

In some embodiments, pipetting mechanism 60 further comprises a first lift assembly and a second lift assembly. The single channel pipetting assembly 62 is mounted on the transfer block 612 by the first lifting assembly. The first lift assembly is used to drive the single channel pipetting assembly 62 up or down in the direction of gravity (i.e., the direction perpendicular to the first horizontal direction and the second moisture direction). The multi-channel pipetting assembly 63 is mounted on the transfer block 612 by a second lifting assembly for driving the multi-channel pipetting assembly 63 to ascend or descend in the direction of gravity.

In this way, when the carriage 612 moves to the gun head carrier 70 in the first horizontal direction and the second horizontal direction under the driving action of the first driving assembly, the first lifting assembly drives the single-channel pipetting assembly 62 to descend in the gravity direction, so that the first mounting head 621 of the single-channel pipetting assembly 62 is matched with the gun head a2 on the gun head carrier 70. Then, the first lifting assembly drives the single-channel pipetting assembly 62 to ascend along the gravity direction, so as to drive the gun head a2 matched with the first mounting head 621 to ascend and separate from the gun head carrier 70, namely, the picking up of the gun head a2 is completed.

When the carrier block 612 moves to the clamping position 31 of the clamping mechanism 30 in the first horizontal direction and the second horizontal direction under the driving action of the first driving assembly, the first lifting assembly drives the single-channel pipetting assembly 62 to descend in the gravity direction, so that the gun head a2 on the first mounting head 621 is inserted into the sampling tube a1, and the single-channel pipetting assembly 62 sucks the liquid in the sampling tube a1 into the gun head a 2. The first lift assembly then drives the single lane pipetting assembly 62 up in the direction of gravity so that the pipette tip a2 on the first mounting head 621 exits the sampling tube a 1.

When the carriage 612 is moved to the nucleic acid isolation mechanism 40 in the first horizontal direction and the second horizontal direction by the driving of the first driving unit, the first raising/lowering unit drives the single-path pipette unit 62 to lower in the gravity direction so that the tip a2 of the first mounting head 621 is inserted into the core nucleic acid isolation tube of the nucleic acid isolation mechanism 40, and at this time, the single-path pipette unit 62 ejects the liquid in the tip a2 into the nucleic acid isolation tube. The first lift assembly then drives the single pass pipetting assembly 62 up in the direction of gravity so that the tip a2 on the first mounting head 621 exits the sampling tube a 1.

When the carriage 612 moves to the waste-tip collection mechanism 80 in the first horizontal direction and the second horizontal direction under the driving action of the first driving assembly, the first lifting assembly drives the single-channel pipetting assembly 62 to descend in the gravity direction, so that the tip a2 on the first mounting head 621 is inserted into the waste-tip collection mechanism 80, and the tip a2 is unmated from the first mounting head 621. Then, the first raising and lowering assembly drives the single-path pipetting assembly 62 to raise in the direction of gravity, so that the first mounting head 621 is separated from the gun head a 2.

When the carriage 612 moves to the gun head carriage 70 in the first horizontal direction and the second horizontal direction under the driving action of the first driving assembly, the second lifting assembly drives the single-channel pipetting assembly 62 to descend in the gravity direction, so that each second mounting head 631 of the multi-channel pipetting assembly 63 is mated with the corresponding gun head a2 on the gun head carriage 70. Then, the multi-channel pipetting assembly 63 is driven by the second lifting assembly to ascend along the gravity direction, so that the gun heads a2 matched with the second mounting heads 631 are driven to ascend and are separated from the gun head carrier 70, and the picking up of the gun heads a2 is completed.

When the carriage 612 is moved to the nucleic acid extracting mechanism 40 in the first horizontal direction and the second horizontal direction by the driving of the first driving unit, the multi-channel pipetting module 63 is driven by the second elevating unit to descend in the gravity direction so that the tips a2 of the second mounting heads 631 are inserted into the plurality of nucleic acid extracting tubes of the nucleic acid extracting mechanism 40, and the multi-channel pipetting module 63 suctions the nucleic acid extracting solutions in the nucleic acid extracting tubes into the tips a 2. Then, the second raising and lowering member drives the multi-channel pipetting member 63 to raise in the direction of gravity so that the pipette tips a2 on the respective second mounting heads 631 are respectively withdrawn from the plurality of nucleic acid extracting tubes.

When the carriage 612 is moved to the reagent carriage 90 in the first horizontal direction and the second horizontal direction by the driving of the first driving unit, the multi-channel pipetting module 63 is driven by the second elevating unit to descend in the gravity direction so that the tips a2 of the second mounting heads 631 are inserted into the plurality of reagent tubes of the reagent carriage 90, and the multi-channel pipetting module 63 ejects the nucleic acid extracting solutions in the tips a2 into the plurality of reagent tubes. Then, the second raising and lowering unit drives the multi-channel pipetting unit 63 to raise in the direction of gravity, so that the tips a2 of the respective second mounting heads 631 exit the plurality of reagent tubes, respectively.

When the carrier base 612 moves to the waste-tip collection mechanism 80 in the first horizontal direction and the second horizontal direction under the driving action of the first driving component, the second lifting and lowering component drives the multi-channel pipetting component 63 to descend in the gravity direction, so that the tips a2 on the second mounting heads 631 are inserted into the waste-tip collection mechanism 80, and the tips a2 are unmated from the second mounting heads 631. Then, the second elevating assembly drives the multi-channel pipetting assembly 63 to ascend in the direction of gravity so that each of the second mounting heads 631 is separated from the tip a 2.

Specifically, in the embodiment, the first lifting assembly includes a first lifting base 623, a second lead screw, a first lifting driving member 622, and a second lead screw nut. The first lifting base 623 is connected to the transfer base 612 in a way of lifting along the gravity direction. The single channel pipetting assembly 62 is mounted on the first lifting and lowering base 623 to ascend or descend in the gravity direction together with the first lifting and lowering base 623. The second lead screw is rotatably connected to the first lifting seat 623 around the axis thereof, and the axis of the second lead screw is parallel to the gravity direction. The first lifting driving member 622 is mounted on the transfer base 612 and is in transmission connection with the second lead screw to drive the second lead screw to rotate around its axis. The second lead screw nut is connected to the second lead screw in a threaded manner and is fixedly connected to the first lifting seat 623, so that the first lifting seat 623 can ascend or descend along with the second lead screw nut in the gravity direction.

Thus, when the single-channel pipetting assembly 62 needs to be lifted, the first lifting driving member 622 drives the second lead screw to rotate, so that the second lead screw nut drives the first lifting seat 623 to ascend or descend in the gravity direction relative to the pipetting seat 612, and then the first lifting seat 623 drives the single-channel pipetting assembly 62 to ascend or descend in the gravity direction. Alternatively, the first elevation drive 622 may employ a motor.

It should be noted that the first lifting assembly is not limited to the screw pair transmission structure to lift the single-channel pipetting assembly 62, and in other embodiments, the first lifting assembly may be implemented by a rack-and-pinion transmission structure, a belt transmission structure, or the like, which is not limited herein.

Specifically, in the embodiment, the second lifting assembly includes a second lifting base 633, a third screw, a second lifting driving member 632 and a third screw nut. The second elevating base 633 is connected to the transferring base 612 in a way of elevating along the gravity direction. The multi-channel pipetting module 63 is mounted on the second elevating base 633 to move in the direction of gravity together with the second elevating base 633. The third screw rod is rotatably connected to the second lifting seat 633 around its axis, and the axis of the third screw rod is parallel to the gravity direction. The second lifting driving member 632 is installed on the transfer base 612, and is in transmission connection with the third screw rod to drive the third screw rod to rotate around its axis. The third screw nut is screwed on the third screw and fixedly connected with the second lifting seat 633, so that the second lifting seat 633 can ascend or descend along with the third screw nut in the gravity direction.

Thus, when the multi-channel pipetting assembly 63 needs to be lifted, the second lifting driving member 632 drives the third lead screw to rotate, so that the third lead screw nut drives the second lifting seat 633 to ascend or descend in the gravity direction relative to the pipetting seat 612, and then the second lifting seat 633 drives the multi-channel pipetting assembly 63 to ascend or descend in the gravity direction. Alternatively, the second elevation driving member 632 may employ a motor.

It should be noted that the second lifting assembly is not limited to the screw assembly transmission structure to lift the multi-channel pipetting assembly 63, and in other embodiments, the second lifting assembly may also be implemented by a rack-and-pinion transmission structure or a belt transmission structure, which is not limited herein.

In the embodiment of the present invention, the door opening mechanism 50 includes a second driving assembly and a door opening robot 521. A second driving assembly is mounted on the mounting frame 10, and the door opening robot 521 is drivingly connected to the second driving assembly, and the second driving assembly is configured to drive the door opening robot 521 to move in the first horizontal direction and the second horizontal direction, so that the door opening robot 521 moves between the sample carrier 20 and the grip site 31 of the grip mechanism 30 to realize the transfer of the sampling tube a 1. The cap opening robot 521 is used to grip the sampling tube a1 and unscrew or tighten the cap of the sampling tube a1 using a rotational motion.

In the embodiment, the second driving assembly includes a second cross member 511 and a moving base 512, the second cross member 511 is movably connected to the mounting frame 10 along a first horizontal direction, the moving base 512 is movably connected to the second cross member 511 along a second horizontal direction, and the door opening robot 521 is mounted on the moving base 512.

Further, the second driving assembly further includes a third driving member 514, a second driving pulley 515, a second driven pulley 516 and a second transmission belt 517. The third driving element 514 is mounted on the mounting frame 10, and the second driving pulley 515 is connected to an output shaft of the third driving element 514 in a transmission manner, so that the third driving element 514 can drive the second driving pulley 515 to rotate. The second driven pulley 516 is rotatably connected to the mounting frame 10, and the second driving pulley 515 and the second driven pulley 516 are arranged at an interval in the first horizontal direction. The second belt 517 is sleeved between the second driving pulley 515 and the second driven pulley 516, and is connected to the second beam 511. Therefore, when the uncapping manipulator 521 needs to be driven to move along the first horizontal direction, the third driving part 514 drives the second driving pulley 515 to rotate, the second driving pulley 515 drives the second transmission belt 517 to sequentially move forward between the second driving pulley 515 and the second driven pulley 516, so that the second transmission belt 517 drives the second beam 511 to move along the first horizontal direction, and then the movable base 512 and the uncapping manipulator 521 are driven to move along the first horizontal direction.

It should be noted that the second driving assembly is not limited to use of a belt transmission structure to realize the movement of the uncovering manipulator 521 along the first horizontal direction, and in other embodiments, the second driving assembly may also use a screw pair transmission structure or a rack-and-pinion transmission structure, and the like, and is not limited herein.

Further, the second driving assembly further includes a fourth lead screw, a fourth driving member 513 and a fourth lead screw nut. The fourth screw rod is rotatably connected to the second cross beam 511 around its own axis, and the axis of the fourth screw rod is parallel to the second horizontal direction. The fourth driving member 513 is mounted on the second cross beam 511, and is in transmission connection with the fourth lead screw to drive the fourth lead screw to rotate around its axis. The fourth screw nut is connected to the fourth screw through a screw thread and is fixedly connected to the movable base 512. Thus, when the uncapping manipulator 521 needs to be driven to move along the second horizontal direction, the fourth driving part 513 drives the fourth lead screw to rotate around the axis of the fourth lead screw, so that the fourth lead screw nut moves along the second horizontal direction, and the moving base 512 and the uncapping manipulator 521 are driven to move along the second horizontal direction. Alternatively, the fourth driving member 513 may employ a motor.

It should be noted that the second driving assembly is not limited to use a screw pair transmission structure to realize the movement of the uncovering manipulator 521 along the second horizontal direction, and in other embodiments, the second driving assembly may also use a belt transmission structure or a rack-and-pinion transmission structure, and the like, which is not limited herein.

In a specific embodiment, the uncovering mechanism 50 further comprises a third lifting assembly, the uncovering manipulator 521 is mounted on the moving base 512 through the third lifting assembly, and the third lifting assembly is used for driving the uncovering manipulator 521 to ascend or descend along the gravity direction.

As such, when the cap opening robot 521 moves above the sample carrier 20 following the moving base 512, the third elevating assembly drives the cap opening robot 521 to descend, so that the cap opening robot 521 can grip the cap of the sampling tube a 1. Then, the third lifting assembly drives the lid opening robot 521 to ascend, so that the gripped sampling tube a1 is separated from the sample carrier 20.

When the uncapping manipulator 521 moves above the clamping position 31 of the clamping mechanism 30 following the moving seat 512, the third lifting assembly drives the uncapping manipulator 521 to descend, so that the uncapping manipulator 521 can insert the sampling tube a1 into the clamping position 31 and be clamped by the clamping mechanism 30. Then, the lid opening robot 521 rotates the lid of the sampling tube a1, thereby unscrewing the lid. After the single channel pipetting assembly 62 has removed the liquid from the sampling tube a1, the lid release robot 521 retightens the lid onto the sampling tube a 1. Then, the gripper mechanism 30 releases the sampling tube a1, and the third elevating assembly drives the uncap robot 521 to ascend, thereby separating the sampling tube a1 from the gripper mechanism 30.

Further, the third lifting assembly includes a third lifting seat 522, a fifth screw, a third lifting driving member 523 and a fifth screw nut. The third lifting stand 522 is connected to the movable stand 512 in a manner of being lifted along the gravity direction. The door opening robot 521 is mounted on the third lifting platform 522 to ascend or descend together with the third lifting platform 522. The fifth lead screw is rotatably connected to the third lifting seat 522 around its own axis, and the axis of the fifth lead screw is parallel to the gravity direction. The third lifting driving member 523 is installed on the transfer base 612 and is in transmission connection with the fifth screw rod to drive the fifth screw rod to rotate around its axis. The fifth lead screw nut is connected to the fifth lead screw through a thread and is fixedly connected with the third lifting seat 522. Thus, when the uncapping manipulator 521 needs to be driven to ascend or descend, the third lifting driving piece 523 drives the fifth lead screw to rotate around the axis of the fifth lead screw, so that the fifth lead screw nut is driven to move along the gravity direction, and the fifth lead screw nut drives the third lifting seat 522 and the uncapping manipulator 521 to move along the gravity direction. Alternatively, the third elevating driving member 523 may employ a motor.

It should be noted that the third lifting assembly is not limited to use of a screw pair transmission structure to achieve the lifting or lowering of the lid opening manipulator 521, and in other embodiments, the third lifting assembly may also use a rack and pinion transmission structure, a belt transmission structure, or the like, and is not limited herein.

Referring to fig. 8, based on the automatic nucleic acid extraction apparatus, the present invention further provides a nucleic acid extraction method using the automatic nucleic acid extraction apparatus in any of the above embodiments, including the steps of:

s10, the uncovering mechanism 50 transfers the sampling tube a1 on the sample carrier 20 to the clamping position 31 of the clamping mechanism 30;

s20, the clamping mechanism 30 clamps the sampling tube a1 at the clamping position 31;

s30, the tube cover on the sampling tube a1 in the clamping position 31 is unscrewed by the cover opening mechanism 50;

s40, moving the single-channel pipetting assembly 62 of the pipetting mechanism 60 to the clamping position 31, and sucking the liquid in the sampling tube a1 of the clamping position 31;

s50, moving the single-channel pipetting assembly 62 of the pipetting mechanism 60 to the nucleic acid extracting mechanism 40 to release the aspirated liquid to the nucleic acid extracting mechanism 40;

s60, the cap release mechanism 50 retightens the cap onto the sampling tube a1 at the grip location 31 and transfers the sampling tube a1 at the grip location 31 to the sample carrier 20.

It should be noted that the above steps S10 to S60 may be sequentially performed in a loop until the liquid in each sampling tube a1 on the sample carrier 20 is transferred to the nucleic acid extracting mechanism 40. And, when the amount of the sample (i.e., the liquid in the sampling tube a1) of one of the nucleic acid extracting mechanisms 40 is sufficient, the nucleic acid extracting mechanism 40 starts the nucleic acid extraction.

Further, in the process of transferring the sampling tube a1 on the sample carrier 20 to the holding position 31 of the holding mechanism 30 by the cover opening mechanism 50, the identification code on the sampling tube a1 is identified by the scanner 81 mounted on the mounting frame 10 to obtain the information of the sampling tube a 1.

Further, the nucleic acid extraction method further comprises the steps of:

s70, the multi-channel pipetting module 63 of the pipetting mechanism 60 is moved to the nucleic acid extracting mechanism 40 to aspirate the nucleic acid extracting solution of the nucleic acid extracting mechanism 40 and transfer the nucleic acid extracting solution into the reagent tube on the reagent carriage 90.

It should be noted that, the steps S70 and S10 to S60 can be performed synchronously, that is, the liquid in the sampling tube a1 and the nucleic acid extracting solution can be transferred synchronously, which is beneficial to improving the detection efficiency.

Further, before step S40, the method further includes the steps of: the single pass pipetting assembly 62 of the pipetting mechanism 60 moves to the tip carrier 70 and picks up the tips a2 on the tip carrier 70.

Further, after step S50, the method further includes the steps of: the single channel pipetting assembly 62 of the pipetting mechanism 60 moves to the waste tip collection mechanism 80 and releases the tips a2 to the waste tip collection mechanism 80.

Further, before the step 70, the method further comprises the steps of: the multi-channel pipetting assembly 63 of the pipetting mechanism 60 moves to the tip carriage 70 and picks up the tips a2 on the tip carriage 70.

Further, after the step 70, the method further comprises the steps of: the multi-channel pipetting assembly 63 of the pipetting mechanism 60 moves to the waste tip collection mechanism 80 and releases the tips a2 to the waste tip collection mechanism 80.

When various reagents (e.g., a lysis solution, a washing solution, an elution solution, and the like) for nucleic acid extraction are not prepackaged in the deep-well plate of the nucleic acid extraction mechanism 40, the method further includes, before step S40:

the single channel pipetting assembly 62 of the pipetting mechanism 60 moves to the reagent carrier 90 and aspirates reagent in the reagent tubes located on the reagent carrier 90;

the single-channel pipetting assembly 62 of the pipetting mechanism 60 moves to the nucleic acid extraction mechanism 40 to release the pipetted reagent into the deep-well plate of the nucleic acid extraction mechanism 40.

The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the utility model. It should be noted that various changes and modifications can be made by those skilled in the art without departing from the spirit of the utility model, and these changes and modifications are all within the scope of the utility model. Therefore, the protection scope of the present patent shall be subject to the appended claims.

Claims (11)

1. An automatic nucleic acid extraction apparatus, characterized by comprising:

a mounting frame (10);

a sample carrier (20) mounted within the mounting frame (10) and for storing a sampling tube (a 1);

the clamping mechanism (30) is arranged in the mounting frame (10) and is provided with a clamping position (31) for clamping or loosening the sampling tube (a 1);

a nucleic acid extraction mechanism (40) which is installed in the mounting frame (10) and is used for extracting nucleic acid;

an uncovering mechanism (50) which is arranged in the mounting frame (10) and is used for transferring a sampling tube (a1) between the sample carrier (20) and the clamping position (31) and uncovering or covering the sampling tube (a1) clamped at the clamping position (31); and

a pipetting mechanism (60) installed in the mounting frame (10) and used for sucking the liquid in the sampling tube (a1) of the clamping position (31) and transferring the liquid to the nucleic acid extraction mechanism (40).

2. The automatic nucleic acid extraction apparatus according to claim 1, further comprising a gun tip carrier (70) mounted in the mounting frame (10), the gun tip carrier (70) being for storing a gun tip;

the pipetting mechanism (60) comprises a first driving component, a transfer seat (612) in driving connection with the first driving component and a single-channel pipetting component (62) arranged on the transfer seat (612); the first driving component is used for driving the transfer seat (612) to move along a horizontal plane and pass through the gun head carrier (70), the clamping position (31) and the nucleic acid extracting mechanism (40), and the single-channel pipetting component (62) is provided with a first mounting head (621) for detachably mounting the gun head;

the first mounting head (621) being capable of picking up tips on the tip carrier (70) when the transfer block (612) is moved to the tip carrier (70); when the transfer seat (612) moves to the clamping position (31), the single-channel pipetting assembly (62) sucks the liquid in the sampling tube (a1) through the gun head; when the transfer base (612) is moved to the nucleic acid extraction mechanism (40), the single-channel pipette unit (62) can discharge the liquid in the tip to the nucleic acid extraction mechanism (40).

3. The automatic nucleic acid extraction apparatus according to claim 2, further comprising a reagent carrier (90) mounted within the mounting frame (10), the reagent carrier (90) being for storing a reagent tube;

the pipetting mechanism (60) further comprises a multi-channel pipetting assembly (63) mounted on the transfer base (612), the multi-channel pipetting assembly (63) having a plurality of second mounting heads (631) for detachably mounting the tips;

the transfer block (612) is also routed through the reagent carrier (90) under the driving action of the first drive assembly; a plurality of the second mounting heads (631) being capable of picking up tips on the tip carrier (70) simultaneously when the transfer seat (612) moves to the tip carrier (70); when the transfer seat (612) is moved to the nucleic acid extraction mechanism (40), the multi-channel pipetting assembly (63) can simultaneously aspirate the nucleic acid extracting solution of the nucleic acid extraction mechanism (40) through a plurality of pipette tips; when the transfer base (612) is moved to the reagent carrier (90), the multi-channel pipetting module (63) can discharge the nucleic acid extracting solutions in the plurality of tips to the plurality of reagent tubes on the reagent carrier (90), respectively.

4. The automatic nucleic acid extraction apparatus according to claim 3, wherein the first driving unit is configured to drive the carriage (612) to move in a first horizontal direction and/or a second horizontal direction intersecting the first horizontal direction.

5. The automatic nucleic acid extracting apparatus according to claim 4, wherein the first driving assembly includes a first beam (611), the first beam (611) is movably connected to the mounting frame (10) in the first horizontal direction, and the transfer base (612) is movably connected to the first beam (611) in the second horizontal direction.

6. The automatic nucleic acid extraction apparatus according to claim 5, wherein the first drive assembly further comprises a first driving member (613), a first driving pulley (614), a first driven pulley (615), and a first driving belt (616);

the first driving piece (613) is mounted on the mounting frame (10), the first driving pulley (614) is in transmission connection with an output shaft of the first driving piece (613), the first driven pulley (615) is rotatably connected on the mounting frame (10), and the first driving pulley (614) and the first driven pulley (615) are arranged at intervals along the first horizontal direction; the first transmission belt (616) is sleeved between the first driving pulley (614) and the first driven pulley (615) and is connected with the first beam (611).

7. The automatic nucleic acid extracting apparatus according to claim 5, wherein the first driving unit further comprises a first screw rotatably connected to the first beam (611) about its axis and having an axis parallel to the second horizontal direction, a second driving unit (617) mounted on the first beam (611) and drivingly connected to the first screw, and a first screw nut threadedly connected to the first screw and fixedly connected to the transfer base (612).

8. The automatic nucleic acid extracting apparatus according to claim 3, wherein the pipetting mechanism (60) further comprises a first elevating assembly by which the single channel pipetting assembly (62) is mounted on the transfer stage (612) and a second elevating assembly for driving the single channel pipetting assembly (62) to ascend or descend in the direction of gravity, and the multi-channel pipetting assembly (63) is mounted on the transfer stage (612) by which the second elevating assembly is mounted for driving the multi-channel pipetting assembly (63) to ascend or descend in the direction of gravity.

9. The automatic nucleic acid extracting apparatus according to claim 8, wherein the first elevating assembly includes a first elevating base (623), a second screw, a first elevating driving member (622), and a second screw nut; the first lifting seat (623) is connected to the transfer seat (612) in a lifting manner along the gravity direction, and the single-channel pipetting assembly (62) is installed on the first lifting seat (623); the second screw rod is rotatably connected to the first lifting seat (623) around the axis of the second screw rod, and the axis of the second screw rod is parallel to the gravity direction; the first lifting driving piece (622) is arranged on the transfer seat (612) and is in transmission connection with the second screw rod; the second screw rod nut is in threaded connection with the second screw rod and is fixedly connected with the first lifting seat (623).

10. The automatic nucleic acid extraction apparatus according to claim 8, wherein the second elevation assembly includes a second elevation base (633), a third screw, a second elevation driving member (632), and a third screw nut; the second lifting seat (633) is connected to the transfer seat (612) in a lifting manner along the gravity direction, and the multi-channel pipetting assembly (63) is installed on the second lifting seat (633); the third screw rod is rotatably connected to the second lifting seat (633) around the axis of the third screw rod, and the axis of the third screw rod is parallel to the gravity direction; the second lifting driving piece (632) is arranged on the transfer seat (612) and is in transmission connection with the third screw rod; the third screw rod nut is in threaded connection with the third screw rod and is fixedly connected with the second lifting seat (633).

11. The automatic nucleic acid extraction apparatus according to claim 1, wherein the nucleic acid extraction mechanism (40) includes a plurality of nucleic acid extraction mechanisms (40), and the plurality of nucleic acid extraction mechanisms (40) are capable of performing nucleic acid extraction independently of each other.

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