CN216499451U - Microfluidic droplet generation chip device capable of being connected with injector - Google Patents

Abstract

The utility model relates to a microfluidic droplet generation chip device capable of being connected with an injector, belonging to the technical field of microfluidics. After the pintle of the injector is taken down, the microfluidic droplet generation chip can be connected and fixed with the nipple of the injector through the connecting seat. The liquid drop generating cylinder of the micro-fluidic chip is embedded into the syringe of the injector, and the triangular liquid drop generating unit at the tail end of the liquid drop generating cylinder is soaked by the oil phase which is pumped in advance. Depending on its special shape, when the pumped solution enters the oil phase in the cylinder through the micro-channel and the droplet generation unit, the solution will generate static instability when the narrow channel suddenly enlarges and enters the oil phase, and then the solution shrinks to generate monodisperse droplets. The size of these droplets is determined only by the triangular shape of the droplet-generating unit, so that the microchannels and droplet-generating units can be arranged in series at high density, improving the throughput of droplet preparation while maintaining the uniformity and stability of the droplets. The generated liquid drops can be stored in the injector, so that the liquid drops are convenient to store and transfer.

Description

A microfluidic droplet generation chip device that can be connected to a syringe

technical field

The utility model relates to a microfluidic droplet generation chip device which can be connected to a syringe, and belongs to the technical field of microfluidics.

Background technique

Microfluidic chip (Microfluidic chip) is a science and technology that is mainly characterized by the manipulation of fluids in the micron-scale space, which can realize various functions of conventional chemical or biological experiments through the microfluidic network formed by microchannels.

Compared with conventional experimental techniques, microfluidic technology has the characteristics of easy manipulation, fast reaction speed, low sample consumption, less pollution, low cost and easy integration. It can perform hundreds of experiments in a few minutes or even less time. Simultaneous analysis of samples, and the whole process of sample pretreatment and analysis can be realized online.

Microfluidic chips have been developed from the original micro-total analysis system into a new functional general tool and platform for sample separation analysis, materials, chemistry, biology, medicine and other fields, and are widely used.

However, the use of the chip usually needs to cooperate with a specific centrifugal microfluidic chip detection system including a mechanical control system. The mechanical motor provides power for the dispersion of the droplets, thereby producing uniform and stable water-in-oil droplets. This device can only use amplification tubes as nucleic acid detection containers and carriers, thus greatly limiting the detection efficiency and throughput of this detection mode. And because the chip needs to be used in conjunction with the power unit, storage tube and other supporting equipment, it is difficult to transfer and transport the system, and cross-contamination may also occur during the operation process such as the transfer of droplets, which is difficult to meet the needs of on-site detection of pathogenic microorganisms. .

Utility model content

The technical solution proposed by the utility model to solve the above technical problems is: a microfluidic droplet generation chip device which can be connected to a syringe.

The microfluidic droplet generation chip can be directly connected with a medical syringe, and through the principle technology of step emulsification droplet generation, closely arranged microchannels and special droplet generation units, the utility model can be installed in the syringe injected into the oil phase. After that, without relying on external devices and complicated pipelines, the solution can be directly pumped with a syringe to produce uniform and stable water-in-oil droplets, improve flux, save space and cost, and facilitate the storage, quantification, and storage of droplets. Transfer to improve the efficiency of on-site inspection.

In order to solve the above-mentioned technical problems, the present invention is achieved through the following technical solutions: a microfluidic droplet generation chip device that can be connected to a syringe, comprising a syringe and a microfluidic droplet generation chip that can be connected to the syringe; the syringe includes The nipple 7, the syringe 8 and the piston 9; the microfluidic droplet generation chip includes a suction port 1, a droplet generation cylinder 4, a connection seat 2 and an auxiliary plate 3; the suction port 1 is located on the upper part of the microfluidic droplet generation chip, and the auxiliary The plate 3 is located at the lower part of the microfluidic droplet generation chip, the connection seat 2 is connected with the suction port 1 and the auxiliary plate 3, and the droplet generation cylinder 4 is placed in the connection seat 2 and connected with the suction port 1;

The droplet generation cylinder 4 is in the shape of a sieve, including a microchannel 5 and a droplet generation unit 6. The microchannels 5 are rectangular parallelepipeds, with a number of tens to hundreds, and are arranged in parallel with a high density in the droplet generation cylinder 4; The unit 6 is connected to the microchannel 5 at the end of the microchannel 5, and the shape is a triangular prism, the side height of the triangular prism is h, and the length is a rectangle of w, and the ratio of w to h is 5.5-19:1;

The microfluidic droplet generation chip replaces the position of the original needle plug of the syringe, and is connected to the nipple 7 of the syringe through the connection seat 2;

The droplet generating cartridge 4 is located in the syringe after the microfluidic droplet generating chip is connected to the syringe.

Preferably, the syringe can be a 1 mL, 2 mL, 5 mL, 10 mL or 50 mL syringe commonly used in clinic.

Preferably, the suction port 1 is used for suctioning the solution into the droplet generation cylinder 4 of the microfluidic droplet generation chip.

Preferably, the chip auxiliary plate 3 is used to fix or remove the microfluidic droplet generation chip from the syringe.

A microfluidic droplet generation chip device that can be connected to a syringe, the principle is as follows:

The device includes a syringe and a microfluidic droplet generation chip that can be connected to the syringe. After removing the needle plug of the syringe, the microfluidic droplet generation chip can be connected and fixed with the nipple of the syringe through the connecting seat. The droplet generation barrel of the microfluidic chip is embedded in the syringe barrel, and the triangular droplet generation unit at the end is infiltrated with the oil phase drawn in in advance. Relying on its special shape, when the pumped solution enters the oil phase in the syringe through the microchannel and droplet generation unit, it will cause the solution to generate static instability when the narrow channel suddenly enlarges and enter the oil phase, thereby shrinking. form monodisperse droplets. The size of these droplets is only determined by the triangular shape of the droplet generation unit, so the microchannels and droplet generation units can be continuously arranged with high density, and the uniformity and stability of the droplets can be improved. quantity. The resulting droplets can be kept in a syringe for easy storage and transfer.

Compared with the prior art, the beneficial effects of the present utility model are:

1. The microfluidic droplet generation chip is adapted to commonly used medical syringes. First, in the process of clinical nucleic acid detection, it can easily and quickly produce monodisperse droplets on site without relying on external equipment and complicated pipelines. Simple, easy to install and reduce production costs. The second is that there is no need to transfer the droplets to other containers, and the droplets that can be generated in one step are directly stored and quantified in the syringe, reducing the chance of contamination and ensuring safety.

2. Using the step emulsification technology, through the high-density arrangement of microchannels and droplet generation units, the chip space is fully utilized to improve the droplet generation flux, the droplet size is uniform, and the properties are stable.

Description of drawings

The utility model will be further described below in conjunction with the accompanying drawings.

Figure 1 is an external schematic diagram of a microfluidic droplet generation chip device that can be connected to a syringe.

FIG. 2 is an external schematic view of the droplet generation chip and the syringe.

Figure 3 is a cross-sectional view of a microfluidic droplet generation chip device to which a syringe can be attached.

FIG. 4 is an external schematic view of the droplet generation chip.

FIG. 5 is a schematic diagram of the structure of a droplet generation chip.

Wherein: 1-suction port; 2-connecting seat; 3-auxiliary plate; 4-droplet generating cylinder; 5-microchannel; 6-droplet generating unit; 7-nipple; 8-syringe; 9-piston.

Detailed ways

Example 1

A microfluidic droplet generation chip device that can be connected to a syringe, comprising a syringe and a microfluidic droplet generation chip that can be connected to the syringe; the syringe includes a nipple 7, a syringe 8, and a piston 9; the microfluidic droplet generation chip It includes a suction port 1, a droplet generation cylinder 4, a connecting seat 2 and an auxiliary plate 3; the droplet generation cylinder 4 is sieve-shaped and includes a microchannel 5 and a droplet generation unit 6. The microchannel 5 is a cuboid, and the number is tens to Hundreds of them are arranged in parallel with high density in the droplet generation cylinder 4; the droplet generation unit 6 is connected to the microchannel 5 at the end of the microchannel 5, and the shape is a triangular prism, the side height of the triangular prism is h, and the length is w Rectangle, the ratio of w to h is 5.5-19:1;

The suction port 1 is located at the upper part of the microfluidic droplet generation chip, the auxiliary plate 3 is located at the lower part of the microfluidic droplet generation chip, the droplet generation cylinder 4 is placed in the connection seat 2 and connected to the suction port 1, and the auxiliary plate 3 is connected to the connection seat 2 connected;

The microfluidic droplet generation chip replaces the position of the original needle plug of the syringe, and is connected to the nipple 7 of the syringe through the connection seat 2;

The droplet generating cartridge 4 is located in the syringe after the microfluidic droplet generating chip is connected to the syringe.

The syringe can be 1mL, 2mL, 5mL, 10mL or 50mL commonly used in clinical practice.

The suction port 1 is used for suctioning the solution into the droplet generation cylinder 4 of the microfluidic droplet generation chip.

The chip auxiliary board 3 is used to fix or remove the chip from the syringe.

The syringe must pre-aspirate a certain volume of oil phase liquid.

The oil phase is mineral oil.

8% mass fraction of surfactant was added to the oil phase to increase the stability of stable droplets.

First, pull the plunger of the syringe to suck a sufficient volume of oil into the syringe, then replace the needle plug, replace it with the microfluidic droplet generation chip of this embodiment, and fix the connection seat 2 of the chip on the nipple 7 of the syringe. At this time, the droplet generating cartridge of the chip is embedded in the oil phase. The required solution is drawn from the suction head into the internal space of the chip by pulling the plunger of the syringe, and the liquid is sucked into the droplet generation cylinder under the suction force, passing through the microchannels arranged in a row, and at the junction of the droplet generation unit and the oil phase, due to static The instability produces monodisperse droplets. These monodisperse droplets are stored in the syringe. Next, you can choose to remove the chip and re-install the pin to seal it for storage. These droplets can be pushed out of the syringe for use.

The present invention is not limited to the above embodiments. All technical solutions formed by using equivalent replacements fall within the protection scope required by the present invention.

Claims (4)

1. A microfluidic droplet generation chip device connectable to a syringe, comprising:

comprises an injector and a microfluidic droplet generation chip which can be connected with the injector; the injector comprises a nipple (7), a needle cylinder (8) and a piston (9); the microfluidic droplet generation chip comprises an attraction port (1), a droplet generation cylinder (4), a connecting seat (2) and an auxiliary plate (3); the suction port (1) is positioned at the upper part of the microfluidic droplet generation chip, the auxiliary plate (3) is positioned at the lower part of the microfluidic droplet generation chip, the connecting seat (2) is connected with the suction port (1) and the auxiliary plate (3), and the droplet generation cylinder (4) is arranged in the connecting seat (2) and is connected with the suction port (1); the liquid drop generating cylinder (4) is in a sieve shape and comprises a micro-channel (5) and a liquid drop generating unit (6), the micro-channel (5) is a cuboid, the number of the micro-channel is dozens to hundreds, and the micro-channel is arranged in the liquid drop generating cylinder (4) in parallel at high density; the liquid drop generating unit (6) is connected with the micro-channel (5) at the tail end of the micro-channel (5), the shape of the liquid drop generating unit is triangular prism, the height of the side surface of the triangular prism is h, the length of the triangular prism is w, the ratio of w to h is 5.5-19: 1;

the micro-fluidic liquid drop generating chip replaces the position of the original pintle of the injector and is connected with the nipple (7) of the injector through the connecting seat (2);

the liquid drop generating cylinder (4) is arranged in the needle cylinder after the microfluidic liquid drop generating chip is connected with the injector.

2. The microfluidic droplet generation chip device connectable to a syringe according to claim 1, wherein: the syringe may be 1mL, 2mL, 5mL, 10mL or 50mL of a syringe which is generally used clinically.

3. The microfluidic droplet generation chip device connectable to a syringe according to claim 1, wherein: the suction port (1) is used for sucking the solution into a liquid drop generating cylinder (4) of the microfluidic liquid drop generating chip.

4. The microfluidic droplet generation chip device connectable to a syringe according to claim 1, wherein: the chip auxiliary plate (3) is used for fixing or detaching the microfluidic droplet generation chip to or from the injector.

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