CN214937511U - Temperature control system of column type detection card - Google Patents
Temperature control system of column type detection card Download PDFInfo
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- CN214937511U CN214937511U CN202121372366.3U CN202121372366U CN214937511U CN 214937511 U CN214937511 U CN 214937511U CN 202121372366 U CN202121372366 U CN 202121372366U CN 214937511 U CN214937511 U CN 214937511U
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- column type
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Abstract
The utility model provides a temperature control system of column type detection card relates to external diagnosis nucleic acid detection field. The technical problems that the existing temperature control system is low in nucleic acid detection efficiency and high in heat dissipation pressure are mainly solved. The column type detection card comprises a cracking area, a washing area and a reaction area; this temperature control system includes constant temperature module, alternating temperature module and controller, the controller is used for control the constant temperature module with alternating temperature module works as after nucleic acid check out test set is loaded to the column type detection card, the constant temperature module with the washing district heat transfer cooperation of column type detection card, the alternating temperature module with the reaction zone heat transfer cooperation of column type detection card.
Description
Technical Field
The utility model relates to an external diagnosis nucleic acid detects the field, especially relates to a temperature control system of column type detection card.
Background
There is only one heating module in the existing nucleic acid detecting apparatus. Some of them only control the temperature of the amplification reaction, and some of them control the temperature of the amplification reaction and the temperature of the washing process by using the same heating module.
The defects of long detection period and low efficiency of the nucleic acid detection devices are common. In addition, for the equipment which adopts the same heating module to control the temperature of the amplification reaction and the temperature in the washing process, the defects of large heat dissipation pressure and low temperature change speed exist because the heating area is large.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a column type detects temperature control system of card to improve nucleic acid detection efficiency, and alleviate the radiating pressure.
In order to achieve the above purpose, the utility model adopts the following technical scheme:
a temperature control system of a column type detection card, wherein the column type detection card comprises a cracking zone, a washing zone and a reaction zone; the temperature control system comprises a constant temperature module, a variable temperature module and a controller, wherein the controller is used for controlling the constant temperature module and the variable temperature module, when the column type detection card is loaded on the nucleic acid detection equipment, the constant temperature module is in heat transfer fit with a washing area of the column type detection card, and the variable temperature module is in heat transfer fit with a reaction area of the column type detection card.
In the temperature control system of the column type detection card described above, preferably, the thermostatic module is further in heat transfer cooperation with the cleavage region of the column type detection card after the column type detection card is loaded into the detection device.
In the temperature control system of the column type detection card, preferably, the heating area of the temperature changing module is smaller than the heating area of the constant temperature module.
In the temperature control system of the column type detection card, preferably, the thermostatic module and the magnetic bead driving magnet are respectively arranged at two opposite sides of the column type detection card.
In the temperature control system of the column type detection card described above, preferably, the constant temperature module includes:
a first inner heat-conducting plate configured to be opposite to one side of a washing zone of the column-type detection card;
a first outer heat sink disposed at an outer side portion of the first inner heat transfer plate; and
and the first heating body is configured between the first inner heat conducting plate and the first outer heat radiating plate.
In the temperature control system of the column type detection card described above, preferably, the thermostatic module further includes:
a second inner heat-conducting plate configured to be opposite to one side portion of the cracking zone of the column type detection card, and having a lower end heat-conductively combined with the first inner heat-conducting plate; and
and a second outer heat dissipation plate disposed at an outer side portion of the second inner heat conduction plate.
In the temperature control system of the column type detection card, preferably, the outer surface of the first outer heat dissipation plate includes heat dissipation fins, a hollow portion is disposed at a position corresponding to the heat dissipation fins at the bottom of the second outer heat dissipation plate, heat dissipation holes are disposed at an outer side portion of the second outer heat dissipation plate, and a hollow structure communicating the hollow portion and the heat dissipation holes is disposed inside the second outer heat dissipation plate.
In the temperature control system of the column type detection card, preferably, the temperature change module includes:
a third inner heat conducting plate, which is provided with a circular truncated cone hole therein, wherein the circular truncated cone hole is configured to be embedded with a reaction area of the column type detection card;
a third outer heat sink disposed at an outer side portion of the third inner heat transfer plate;
a second heating element disposed between the third inner heat-conducting plate and the third outer heat-radiating plate;
a conduit extending through the third outer heat sink; and
and an auxiliary radiator arranged on the pipeline.
In the temperature control system of the column type detection card, preferably, the auxiliary heat radiator is a heat radiation pipe, and the first heating element and the second heating element are semiconductor cooling fins.
In the temperature control system of the column type detection card, preferably, the temperature control system is provided with two constant temperature modules and two temperature change modules, inner heat conduction plates of the two constant temperature modules are opposite, inner heat conduction plates of the two temperature change modules are opposite, the two constant temperature modules and the two temperature change modules are combined into a whole through a connecting plate at the end part, and the two constant temperature modules and the two temperature change modules correspondingly provide temperature control of the two sets of column type detection cards.
Compared with the prior art, the utility model discloses following beneficial effect has at least:
after the heating module is divided into the constant temperature module and the variable temperature module, the working temperature of the constant temperature module is not high, and the heating area of the variable temperature module with higher working temperature is reduced, so that the heat dissipation pressure is reduced.
After the temperature-changing module is divided into two heating modules, the heating area of the temperature-changing module is reduced, the temperature rising and falling speed is effectively increased, the controllable speed of temperature rising is 4-8 ℃/s and the controllable speed of temperature falling is 3-5 ℃/s in the conventional mode-adjusting effect; meanwhile, the temperature-changing module can independently operate, and when the DNA nucleic acid is detected, the activation of hot start enzyme and the sinking of the primer probe can be completed in the nucleic acid extraction period, so that the detection time is shortened. Therefore, the detection time can be effectively shortened, and the detection efficiency is improved.
In addition, when the constant temperature module includes the second inner heat-conducting plate and the second outer heat-dissipating plate, the cracking liquid can be heated, and the cracking effect can be improved.
Drawings
FIG. 1 is a schematic view of a column-type test card;
FIG. 2 is a schematic distribution diagram of a constant temperature module and a variable temperature module of the temperature control system;
FIG. 3 is a block circuit diagram of a temperature control system;
FIG. 4 is a perspective view of a nucleic acid detecting apparatus;
FIG. 5 is a schematic structural view of a constant temperature module and a temperature change module in the nucleic acid detecting apparatus;
FIG. 6 is a schematic view of two constant temperature modules and two variable temperature modules in combination;
FIG. 7 is a schematic structural view of a first outer heat sink;
FIG. 8 is a schematic structural view of the first inner heat-conducting plate and the second inner heat-conducting plate;
fig. 9 is a schematic structural view of a second external heat dissipation plate;
FIG. 10 is a schematic structural view of the third inner heat-conducting plate;
FIG. 11 is a schematic structural view of a third outer heat sink;
reference numerals:
1. a constant temperature module;
11. a first outer heat sink; 12. a first heat generating body; 13. a first inner heat-conducting plate; 14. a second outer heat dissipation plate; 15. heat dissipation holes; 16. a hollow structure; 17. a second inner heat-conducting plate; 18. heat dissipation fins;
2. a temperature changing module;
21. a third outer heat sink; 22. a second heating element; 23. a third inner heat-conducting plate; 24. a circular truncated cone hole; 25. a pipeline;
3. a connecting plate;
4. a containing groove;
5. a column type detection card;
51. a cleavage zone; 52. a washing zone; 53. a reaction zone;
6. a drive magnet;
7. magnetic beads;
8. and a controller.
Detailed Description
The present invention will be further explained with reference to the drawings and examples.
Referring to fig. 1, the column type detection card 5 includes a cleavage region 51, a washing region 52, and a reaction region 53.
Referring to fig. 2 and 3, the temperature control system in the nucleic acid detecting apparatus includes a constant temperature module 1, a temperature changing module 2, and a controller 8, where the controller 8 is electrically connected to the constant temperature module 1 and the temperature changing module 2, and is configured to control the constant temperature module 1 and the temperature changing module 2. When the column type detection card 5 is loaded into the nucleic acid detection apparatus, the constant temperature module 1 is in heat transfer cooperation with the washing zone 52 and the cleavage zone 51 of the column type detection card 5, and the temperature change module 2 is in heat transfer cooperation with the reaction zone 53 of the column type detection card 5. Specific heat transfer means that may be employed include, but are not limited to, conduction and radiation.
The heating area of the temperature changing module 2 is smaller than that of the constant temperature module 1.
The constant temperature module 1 and the driving magnet 6 of the magnetic bead 7 are respectively arranged at two opposite sides of the column type detection card 5, so that the magnetic adsorption of the driving magnet 6 to the magnetic bead 7 is not interfered.
The structure of one specific nucleic acid detecting apparatus is shown in FIGS. 4 to 11.
Referring to fig. 4 to 11, the thermostat module 1 includes: a first inner heat-conducting plate 13, a first outer heat-radiating plate 11 and a first heat-generating body 12. The first inner heat conduction plate 13 is disposed to face one side portion of the washing section 52 of the column type detection card 5, the first outer heat radiation plate 11 is disposed on the outer side portion of the first inner heat conduction plate 13, and the first heat generating body 12 is disposed between the first inner heat conduction plate 13 and the first outer heat radiation plate 11. Specifically, the first heating element 12 is a semiconductor refrigeration sheet, the back of the first inner heat conduction plate 13 is provided with an accommodating groove 4, the first heating element 12 is embedded in the accommodating groove 4, the first outer heat dissipation plate 11 is combined with the first inner heat conduction plate 13 through the connecting plate 3 at the end part, and the first heating element 12 is respectively attached to the first outer heat dissipation plate 11 and the first inner heat conduction plate 13. The first outer heat sink 11 includes heat dissipation fins 18 on its outer surface.
The thermostatic module 1 also comprises a second inner heat-conducting plate 17 and a second outer heat-dissipating plate 14. The second inner heat-conducting plate 17 is disposed to be opposed to one side portion of the cleavage section 51 of the column type detection card 5, and has a lower end heat-conductively coupled to the first inner heat-conducting plate 13. The second outer heat dissipation plate 14 is disposed on the outer side of the second inner heat conduction plate 17. The second inner heat-conducting plate 17 and the second outer heat-dissipating plate 14 are combined together by the connecting plate 3 at the end portion, and are combined with the first inner heat-conducting plate 13 and the first outer heat-dissipating plate 11. In use, the first inner heat-conducting plate 13 conducts heat energy to the second inner heat-conducting plate 17 to effect heating of the pyrolysis zone 51.
A hollow part is arranged at the bottom of the second outer heat dissipation plate 14 corresponding to the position of the heat dissipation fins 18, a heat dissipation hole 15 is arranged at the outer side part of the second outer heat dissipation plate 14, and a hollow structure 16 communicated with the hollow part and the heat dissipation hole 15 is arranged in the second outer heat dissipation plate 14, so that heat dissipation is facilitated.
Wherein the second inner heat-conducting plate 17 and the first inner heat-conducting plate 13 can be two plates or can be the same plate.
The temperature change module 2 comprises: a third inner heat-conducting plate 23, a third outer heat-radiating plate 21, and a second heat-generating body 22.
The third inner heat conducting plate 23 is internally provided with a circular platform hole 24, and the circular platform hole 24 is configured to be embedded with the reaction zone 53 of the column type detection card 5; the third outer heat radiation plate 21 is disposed at an outer side portion of the third inner heat conduction plate 23; the second heat generating element 22 is disposed between the third inner heat transfer plate 23 and the third outer heat dissipation plate 21; the third outer heat sink 21 has a conduit 25 therethrough; the heat dissipation pipe is arranged in the pipeline 25 and connected with the air cooling system or the liquid cooling system, so that the temperature reduction of the temperature changing module 2 can be accelerated. The second heating element 22 is a semiconductor refrigeration sheet, the back of the third inner heat conduction plate 23 is provided with a containing groove 4, and the second heating element 22 is embedded in the containing groove 4 and is respectively attached to the third inner heat conduction plate 23 and the third outer heat dissipation plate 21. The third inner heat-conducting plate 23 and the third outer heat-radiating plate 21 are combined into a whole by the connecting plates 3 at both ends, and are combined into a whole with the constant temperature module 1 by the connecting plates 3.
Referring to fig. 5 and 6, the temperature control system of the nucleic acid detecting apparatus has two constant temperature modules 1 and two temperature change modules 2, wherein the inner heat conducting plates of the two constant temperature modules 1 are opposite, the inner heat conducting plates of the two temperature change modules 2 are opposite, the two constant temperature modules 1 and the two temperature change modules 2 are combined into a whole through a connecting plate 3 at the end part, and the two constant temperature modules 1 and the two temperature change modules 2 correspondingly provide temperature control of two sets of column type detecting cards 5.
The working sequence of the temperature control system is as follows:
the constant temperature module 1 heats the cracking solution and the washing solution to improve the cracking effect;
the temperature changing module 2 operates independently and controls the temperature of the amplification reaction zone of the detection card. The activation of hot start enzyme during nucleic acid extraction and the sinking of primer probe can be utilized for DNA nucleic acid detection, so that the detection time is shortened.
In nucleic acid detection, the heating temperature of the lysis solution and the washing solution is low, while the temperature required for the amplification reaction is high. In the above embodiment, after the two heating modules, namely the constant temperature module 1 and the variable temperature module 2, are adopted, the working temperature of the constant temperature module 1 is not high, and the heating area of the variable temperature module 2 with a higher working temperature is reduced, so that the heat dissipation pressure is reduced.
In addition, the heating area of the temperature changing module 2 is reduced, the temperature rising and falling speed is effectively improved, the controllable speed of temperature rising is 4-8 ℃/s, and the controllable speed of temperature falling is 3-5 ℃/s. After the two heating modules are adopted, the temperature changing module 2 can be independently operated, and the activation of hot start enzyme and the sinking of a primer probe can be completed in the nucleic acid extraction period during the detection of DNA nucleic acid, so that the detection time is shortened. These make it possible to effectively shorten the detection time and improve the detection efficiency.
The present invention has been described in detail with reference to the specific embodiments, and the detailed description is only for assisting the skilled person in understanding the content of the present invention, and can not be understood as the limitation of the protection scope of the present invention. Various decorations, equivalent changes and the like which are performed on the scheme by the technical personnel in the field under the conception of the invention are all included in the protection scope of the invention.
Claims (10)
1. The utility model provides a temperature control system of column type detection card which characterized in that:
the column type detection card (5) comprises a cracking area (51), a washing area (52) and a reaction area (53);
temperature control system includes constant temperature module (1), alternating temperature module (2) and controller (8), controller (8) are used for control constant temperature module (1) with alternating temperature module (2) work as column type detection card (5) load to nucleic acid check out test set after, constant temperature module (1) with washing district (52) heat transfer cooperation of column type detection card (5), alternating temperature module (2) with reaction zone (53) heat transfer cooperation of column type detection card (5).
2. The temperature control system of the spot type detecting card according to claim 1, wherein: when the column type detection card (5) is loaded to the detection equipment, the constant temperature module (1) is also in heat transmission fit with the cracking zone (51) of the column type detection card (5).
3. The temperature control system of the spot type detecting card according to claim 1, wherein: the heating area of the temperature changing module (2) is smaller than that of the constant temperature module (1).
4. The temperature control system of the spot type detecting card according to claim 1, wherein: the constant temperature module (1) and the driving magnet (6) of the magnetic bead (7) are respectively arranged at two opposite sides of the column type detection card (5).
5. The temperature control system of the column detection card according to claim 1, characterized in that the thermostatic module (1) comprises:
a first inner heat-conducting plate (13) arranged opposite to one side of the washing zone (52) of the column-type detection card (5);
a first outer heat dissipation plate (11) disposed on an outer side portion of the first inner heat conduction plate (13); and
and a first heat generating body (12) disposed between the first inner heat-conducting plate (13) and the first outer heat-radiating plate (11).
6. The temperature control system of the column detection card according to claim 5, characterized in that the thermostatic module (1) further comprises:
a second inner heat-conducting plate (17) disposed opposite to one side of the cracking zone (51) of the column-type detection card (5) and having a lower end in heat-conductive combination with the first inner heat-conducting plate (13); and
and a second outer heat dissipation plate (14) disposed on the outer side of the second inner heat conduction plate (17).
7. The temperature control system of the column type detection card according to claim 6, wherein the outer surface of the first outer heat dissipation plate (11) comprises heat dissipation fins (18), the bottom of the second outer heat dissipation plate (14) is provided with a hollow portion corresponding to the position of the heat dissipation fins (18), the outer side of the second outer heat dissipation plate (14) is provided with heat dissipation holes (15), and the inside of the second outer heat dissipation plate (14) is provided with a hollow structure (16) communicating the hollow portion and the heat dissipation holes (15).
8. The temperature control system of the spot detection card according to claim 6, wherein the temperature change module (2) comprises:
a third inner heat-conducting plate (23) having a circular truncated cone hole (24) therein, the circular truncated cone hole (24) being configured to be fitted with a reaction region (53) of a column-type detection card (5);
a third outer heat radiation plate (21) disposed at an outer side portion of the third inner heat conduction plate (23);
a second heating element (22) disposed between the third inner heat-conducting plate (23) and the third outer heat-radiating plate (21);
a conduit (25) extending through the third outer heat sink (21); and
an auxiliary radiator arranged in the duct (25).
9. The temperature control system of the column type probe card according to claim 8, wherein the auxiliary heat radiator is a heat radiating pipe, and the first and second exothermic bodies (12, 22) are semiconductor cooling fins.
10. The temperature control system of column type detection card according to claim 9, characterized in that, there are two said constant temperature modules (1) and two said temperature change modules (2), the inner heat conducting plates of two said constant temperature modules (1) are opposite, the inner heat conducting plates of two said temperature change modules (2) are opposite, two said constant temperature modules (1) and two said temperature change modules (2) are combined into a whole through the connecting plate (3) of the end, two said constant temperature modules (1) and two said temperature change modules (2) correspondingly provide the temperature control of two sets of column type detection card (5).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121372366.3U CN214937511U (en) | 2021-06-18 | 2021-06-18 | Temperature control system of column type detection card |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121372366.3U CN214937511U (en) | 2021-06-18 | 2021-06-18 | Temperature control system of column type detection card |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN214937511U true CN214937511U (en) | 2021-11-30 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202121372366.3U Active CN214937511U (en) | 2021-06-18 | 2021-06-18 | Temperature control system of column type detection card |
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| CN (1) | CN214937511U (en) |
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2021
- 2021-06-18 CN CN202121372366.3U patent/CN214937511U/en active Active
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