CN214668096U

CN214668096U - Sample detection device and suction mechanism thereof

Info

Publication number: CN214668096U
Application number: CN202120081618.0U
Authority: CN
Inventors: 丁建文; 彭永乐
Original assignee: AVE Science and Technology Co Ltd
Current assignee: AVE Science and Technology Co Ltd
Priority date: 2021-01-12
Filing date: 2021-01-12
Publication date: 2021-11-09
Anticipated expiration: 2031-01-12

Abstract

The utility model relates to a sample detection device and suction mechanism thereof, suction mechanism include evacuation piece, first exhaust line and first exhaust line. The first air suction pipeline is communicated with an air suction end of the vacuumizing piece and the enricher, and one end of the first air exhaust pipeline is communicated with the enricher. And opening the first valve, closing the second valve and the third valve and starting the vacuumizing piece, so that negative pressure can be formed in the first negative pressure cavity. When sample enrichment is required, the second valve is opened to communicate the first negative pressure chamber with the concentrator, and negative pressure can be formed in the concentrator. After the enrichment is finished, the second valve is closed and the third valve is opened, so that the air pressure in the enricher can be recovered to be normal to smoothly perform sample application. Because the first negative pressure cavity can form and maintain negative pressure in advance, when sample enrichment is carried out, negative pressure can be formed in the enrichment device instantly only by opening the second valve, and waiting time is obviously reduced. Therefore, the time required for sample enrichment is reduced, and the efficiency of sample detection is improved.

Description

Sample detection device and suction mechanism thereof

Technical Field

The utility model relates to a medical treatment detects technical field, in particular to sample detection device and suction mechanism thereof.

Background

In the diagnosis of diseases, the most basic procedure is to detect samples of blood, body fluids, secretions, excretions and casts of a patient. The sample is usually collected and transferred in the form of suspension, and in order to improve the detection rate of the visible components in the sample, the enrichment and concentration treatment on the suspension sample is required.

One relatively novel method of sample enrichment is to create a negative pressure within the concentrator by drawing a vacuum and using the negative pressure to cause the suspension sample to flow into the concentrator. Wherein, one end of the enricher is provided with a blocking piece, such as a filter screen, and the formed components in the enricher are blocked by the blocking piece along with the continuous flow of the sample, so that the enrichment is completed on the surface of the blocking piece.

Currently, a vacuum pump is generally used to directly suck the enricher to form negative pressure. However, the starting and the air extraction of the vacuum pump are both slow processes, and the vacuum pump needs to work for a period of time to enable the negative pressure in the enricher to meet the requirement. This results in a longer time required for sample enrichment, which reduces the efficiency of sample detection.

SUMMERY OF THE UTILITY MODEL

In view of the above, it is desirable to provide a sample detection device and a pumping mechanism thereof, which can improve the efficiency of sample detection.

A suction mechanism for evacuating a concentrator, the suction mechanism comprising:

vacuumizing the part;

the first air suction pipeline is communicated with an air suction end of the vacuumizing piece and the enricher and comprises a first negative pressure cavity, a first valve and a second valve, the first valve is arranged between the first negative pressure cavity and the vacuumizing piece, and the second valve is arranged between the first negative pressure cavity and the enricher; and

and one end of the first exhaust pipeline is communicated with the enricher, and a third valve is arranged on the first exhaust pipeline.

In one embodiment, the waste liquid recovery device further comprises a waste liquid recovery pipeline, the waste liquid recovery pipeline comprises a waste liquid container, a suction pump and a fourth valve, an input end of the suction pump is communicated with the first negative pressure cavity, an output end of the suction pump is communicated with the waste liquid container, and the fourth valve is arranged between the suction pump and the first negative pressure cavity.

In one embodiment, a recovery branch extends from a region of the waste liquid recovery pipeline between the fourth valve and the suction pump, and a fifth valve is arranged on the recovery branch.

In one embodiment, the device further comprises a second air suction pipeline for communicating the air suction end of the vacuum pumping piece with the slide sucker, wherein the second air suction pipeline comprises a second negative pressure cavity, a sixth valve and a seventh valve, the sixth valve is arranged between the second negative pressure cavity and the vacuum pumping piece, and the seventh valve is arranged between the second negative pressure cavity and the slide sucker.

In one embodiment, the device further comprises a second exhaust pipeline, one end of the second exhaust pipeline is communicated with the slide sucker, and an eighth valve is arranged on the second exhaust pipeline.

In one embodiment, the eighth valve is a three-way valve, and the eighth valve has a gas inlet and two gas outlets, the gas inlet is communicated with the gas outlet of the vacuum pumping element, and the two gas outlets are respectively communicated with the atmosphere and the second exhaust pipeline.

In one embodiment, the vacuum-pumping device further comprises a third exhaust pipeline for communicating the pumping end of the vacuum-pumping part with the atmosphere, and a ninth valve is arranged on the third exhaust pipeline.

In one embodiment, the first pumping line, the second pumping line and the third exhaust line are partially overlapped at one end close to the vacuum pumping part.

In one embodiment, the first negative pressure cavity and the second negative pressure cavity are both provided with pressure sensors.

A sample testing device comprising:

a suction mechanism as described in any of the above preferred embodiments;

the enrichment device is communicated with the first air suction pipeline and can move under the driving of the servo mechanism.

According to the sample detection device and the suction mechanism thereof, the first valve is opened, the second valve and the third valve are closed, and the vacuumizing piece is started, so that negative pressure can be formed in the first negative pressure cavity. When sample enrichment is required, the second valve is opened to communicate the first negative pressure chamber with the concentrator, and negative pressure can be formed in the concentrator. After the enrichment is finished, the second valve is closed and the third valve is opened, so that the air pressure in the enricher can be recovered to be normal to smoothly perform sample application. Because the first negative pressure cavity can form and maintain negative pressure in advance, when sample enrichment is carried out, negative pressure can be formed in the enrichment device instantly only by opening the second valve, and waiting time is obviously reduced. Therefore, the time required for sample enrichment is reduced, and the efficiency of sample detection is improved.

Drawings

In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present application, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.

Fig. 1 is a block diagram of a pumping mechanism according to a preferred embodiment of the present invention.

Detailed Description

In order to make the above objects, features and advantages of the present invention more comprehensible, embodiments of the present invention are described in detail below with reference to the accompanying drawings. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein, as those skilled in the art will be able to make similar modifications without departing from the spirit and scope of the present invention.

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", and the like, indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.

In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," and "fixed" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.

In the present application, unless expressly stated or limited otherwise, the first feature may be directly on or directly under the second feature or indirectly via intermediate members. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.

It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and the like as used herein are for illustrative purposes only and do not denote a unique embodiment.

Referring to fig. 1, the present invention provides a sample detection device and a pumping mechanism 100. The sample detection device includes a pumping mechanism 100, an enricher 200, and a servo mechanism (not shown).

The enricher 200 can be moved under the urging of a servo. The suction mechanism 100 is capable of creating a negative pressure within the concentrator 200, with a barrier (not shown) on the concentrator 200. When the concentrator 200 is extended into the sample, the negative pressure will cause the suspension sample to flow into the concentrator 200, and the formed components therein will be blocked by the barrier, thereby completing the enrichment at the surface of the barrier. The sample testing device also typically includes a slide tip 300, the slide tip 300 being capable of movement under the drive of a servo mechanism. The slide tip 300 is capable of aspirating and releasing a slide and is capable of transferring the slide to a sample application site under the drive of a servo mechanism.

Moreover, the concentrator 200 can move towards the slide at the spotting position under the driving of the servo mechanism, so as to perform spotting. Then, the servo mechanism can also drive the slide after sample application to move to a microscopic examination position. The microscopic examination position is generally correspondingly provided with a microscope, a detection probe and the like, so that the detection of the sample is finally completed.

The pumping mechanism 100 includes a vacuum pump 110, a first pumping line 120, and a first exhaust line 130.

The evacuation member 110 may be a conventional vacuum generator and vacuum pump, and has an evacuation end and an exhaust end. In this embodiment, the vacuum pumping member 110 is a vacuum pump.

The first pumping line 120 communicates the pumping end of the vacuum pumping member 110 with the enricher 200. The evacuation member 110 evacuates the concentrator 200 through the first suction line 120, thereby creating a negative pressure in the concentrator 200. The first suction line 120 includes a first negative pressure chamber 121, a first valve 122 and a second valve 123. The first sub-pressure chamber 121 is generally a hollow metal chamber structure, and a plurality of connectors are provided thereon to facilitate communication with other components. Obviously, the first suction line 120 also generally includes a connecting pipe, which may be a rubber hose, a metal pipe, or the like.

A first valve 122 is provided between the first sub-atmospheric chamber 121 and the evacuation member 110 and a second valve 123 is provided between the first sub-atmospheric chamber 121 and the concentrator 200. When the first valve 122 is closed, the first negative pressure chamber 121 is isolated from the vacuum pumping member 110; when the second valve 123 is closed, the first negative pressure chamber 121 is isolated from the concentrator 200. When the second valve 123 is closed and the first valve 121 is opened, the vacuum pumping member 110 can pump vacuum to the first negative pressure chamber 121, so as to form negative pressure in the first negative pressure chamber 121. After the negative pressure is formed, the first valve 121 is closed again, so that the negative pressure can be maintained in the first negative pressure chamber 121.

In the present embodiment in particular, the first negative pressure chamber 121 is provided with a pressure sensor 140. The pressure sensor 140 can observe the air pressure in the first negative pressure chamber 121 in real time, thereby conveniently controlling the negative pressure in the first negative pressure chamber 121 to be in a required interval.

One end of the first exhaust line 130 communicates with the enricher 200. The other end of the first exhaust line 130 may be connected to the atmosphere or a positive pressure device. A third valve 131 is provided on the first exhaust line 130. The third valve 131, when closed, may isolate the concentrator 200 from the atmosphere or from the positive pressure device. Specifically, in the present embodiment, the first valve 122, the second valve 123 and the third valve 131 are all pinch valves, and the pinch valves are convenient to install and do not need to damage the structure of the pipeline, so that the pinch valves are convenient to replace. Obviously, the first valve 122, the second valve 123 and the third valve 131 can also be a common two-way valve or other valve structure.

Before the sample enrichment, the first valve 122 may be opened, the second valve 123 and the third valve 131 may be closed, and the vacuum element 110 may be activated to form a negative pressure in the first negative pressure chamber 121. Then, the first valve 122 is closed, so that the first negative pressure chamber 121 can maintain a negative pressure environment. When sample enrichment is required, the second valve 123 is opened. At this point, the first negative pressure chamber 121 is in communication with the concentrator 200, both of which reach a pressure equilibrium instantaneously, so that a negative pressure can be instantaneously created within the concentrator 200.

It can be seen that the evacuation member 110 does not directly evacuate the concentrator 200, but rather a transition in negative pressure is achieved through the first negative pressure chamber 121. Therefore, the evacuation operation can be advanced, so that the waiting time in the sample enrichment process can be significantly reduced.

After completion of sample enrichment, the second valve 123 is closed and the third valve 131 is opened. At this time, the gas pressure in the enricher 200 is restored to normal, so that the spotting can be smoothly performed.

In order to simplify the pipeline structure, the volume of the sample detection device is reduced. In the present embodiment, the first suction line 120 and the first exhaust line 130 partially overlap each other at an end close to the concentrator 200. That is, the first suction line 120 shares a portion of the piping with the first exhaust line 130, and communicates with the enricher 200 through the same interface. Thus, a common portion of the piping may be used for both pumping air to the enricher 200 and for inlet air to the enricher 200.

In this embodiment, the pumping mechanism 100 further comprises a waste liquid recycling pipeline 150, and the waste liquid recycling pipeline 150 comprises a waste liquid container 151, a pumping pump 152 and a fourth valve 153. Wherein, the input end of the suction pump 152 is communicated with the first negative pressure cavity 121, the output end is communicated with the waste liquid container 151, and the fourth valve 153 is arranged between the suction pump 152 and the first negative pressure cavity 121.

In the process of sample enrichment and vacuum pumping of the first negative pressure cavity 121, the fourth valves 153 are all in a closed state, and the waste liquid recovery pipeline 150 is isolated. After the sample is completely enriched and spotted, more waste fluid is accumulated in the enricher 200. At this time, the fourth valve 153 and the second valve 123 are opened, so that the waste liquid recycling pipeline 150 is communicated with the enricher 200. Then, the suction pump 152 is started, and the waste liquid in the enricher 200 can enter the waste liquid container 151 through the first negative pressure cavity 121 and the suction pump 152 for storage, thereby facilitating subsequent uniform treatment.

It can be seen that the waste liquid in the enricher 200 can be automatically collected through the waste liquid recovery pipeline 150, so that the enricher 200 is not required to be cleaned once every time the sample application is completed, the operation is more convenient, and the detection efficiency of the sample detection device is further improved.

Similarly, the fourth valve 153 is also typically a pinch valve, and the suction pump 152 may alternatively be a peristaltic pump.

Further, in this embodiment, a recycling branch (not shown) extends from a region of the waste liquid recycling line 150 between the fourth valve 153 and the suction pump 152, and a fifth valve 154 is disposed on the recycling branch.

The recovery branch is generally constituted by a length of pipe, one end of which communicates with the area between the fourth valve 153 and the suction pump 152, and by a fifth valve 154. The fifth valve 154 may also be a pinch valve, normally in a closed position. During the sample detection process, it is often necessary to dye the sample by using a dye solution joint (not shown), which improves the imaging effect. After dyeing is completed, the dyeing solution usually remains at the joint of the dyeing solution.

Therefore, the other end of the pipeline of the recovery branch can be communicated with the dyeing liquid joint. When the fifth valve 154 is opened, the suction pump 152 can suck the residual dyeing liquid in the dyeing liquid joint into the waste liquid container 151 for collection, thereby realizing the rotary cleaning of the dyeing liquid joint.

In this embodiment, the suction mechanism 100 further includes a second suction line 160 communicating the suction end of the vacuum 110 with the slide tip 300. The second suction line 160 includes a second negative pressure chamber 161, a sixth valve 162 and a seventh valve 163, the sixth valve 162 is disposed between the second negative pressure chamber 161 and the vacuum pumping member 110, and the seventh valve 163 is disposed between the second negative pressure chamber 161 and the slide sucker 300.

The vacuum pumping member 110 can pump a vacuum to the slide tip 300 through the second suction line 160, so that the slide tip 300 can form a negative pressure to suck a slide. The second pumping line 160 and the first pumping line 120 have substantially the same structure, the second vacuum chamber 161 and the first vacuum chamber 121 have substantially the same structure, and the sixth valve 162 and the seventh valve 163 are also generally pinch valves.

Before the slide suction is performed, the sixth valve 162 may be opened, the seventh valve 163 may be closed, and the vacuum 110 may be activated to form a negative pressure in the second negative pressure chamber 161. Then, the sixth valve 162 is closed, so that the second negative pressure chamber 161 can maintain a negative pressure environment. When the slide is adsorbed, the seventh valve 163 is opened. At this time, the second negative pressure chamber 161 is communicated with the slide tip 300, and the both reach the air pressure balance instantly, so that the slide tip 300 can form the negative pressure instantly. Therefore, the transition of the negative pressure through the second negative pressure chamber 161 can significantly increase the response rate of the slide tip 300 to further improve the detection efficiency.

Specifically in the present embodiment, the second negative pressure chamber 161 is provided with the pressure sensor 140. The pressure sensor 140 can observe the air pressure in the second negative pressure chamber 161 in real time, thereby facilitating the control of the negative pressure in the second negative pressure chamber 161 in a desired interval.

Further, in this embodiment, the suction mechanism 100 further includes a second exhaust line 170 having one end communicating with the slide tip 300, and the eighth valve 171 is disposed on the second exhaust line 170.

Specifically, the second exhaust line 170 has substantially the same structure as the first exhaust line 130. The eighth valve 171 can open or close the second exhaust line 170. The other end of the second exhaust pipe 170 may be connected to the atmosphere or a positive pressure device.

After the second exhaust line 170 is conducted, the negative pressure of the slide tip 300 can be rapidly removed. Therefore, when the slide is moved into position, the eighth valve 171 is opened to allow the slide sucker 300 to quickly release the slide, thereby further improving the efficiency of slide picking and placing.

Furthermore, in the present embodiment, the eighth valve 171 is a three-way valve, and the eighth valve 171 has a gas inlet (not shown) and two gas outlets (not shown), wherein the gas inlet is communicated with the gas outlet of the vacuum pumping device 110, and the two gas outlets are respectively communicated with the atmosphere and the second exhaust pipeline 170.

Specifically, the air inlet of the eighth valve 171 can be alternatively communicated with the two air outlets, and the eighth valve 171 may be an electromagnetic valve or a manual valve. When the slide sucker 300 needs to suck a slide, the air inlet is communicated with the air outlet communicated with the atmosphere, and the air flow discharged from the air outlet end of the vacuumizing part 110 directly enters the atmosphere. When the slide suction head 300 needs to release the slide, the eighth valve 171 is switched to communicate the air inlet with the air outlet of the second air exhaust line 170. At this time, the air flow discharged from the air outlet of the vacuum pumping member 110 enters the slide tip 300, thereby making the slide tip 300 positive pressure to rapidly blow out the slide.

It should be noted that in other embodiments, the eighth valve 171 can be a common pinch valve, and the other end of the second exhaust line 170 is connected to the atmosphere. When the slide pipette tip 300 needs to release the slide, the release of the slide can also be achieved by opening the eighth valve 171.

Furthermore, in the present embodiment, the pumping mechanism 100 further includes a third exhaust pipe 180 for communicating the pumping end of the vacuum pumping device 110 with the atmosphere, and the third exhaust pipe 180 is provided with a ninth valve 181.

The third exhaust line 180 has substantially the same structure as the second exhaust line 170 and the first exhaust line 130. The ninth valve 181 is generally a pinch valve, and can open or close the second exhaust line 170. When the vacuum extractor 110 evacuates the first negative pressure chamber 121 and the second negative pressure chamber 161, the ninth valve 181 is in a closed state.

When the vacuum pumping member 110 is used for pumping vacuum to the first negative pressure cavity 121 and the second negative pressure cavity 161, the air outlet end of the vacuum pumping member 110 can lead out air flow, and the led-out air flow can provide positive pressure for the slide suction head 300 when the slide suction head 300 needs to release a slide. After the negative pressures in the first negative pressure chamber 121 and the second negative pressure chamber 161 reach a desired level, the first valve 122 and the sixth valve 162 are both closed. At this time, in order to keep the vacuum pumping member 110 normally operating, the ninth valve 181 is opened. In this manner, the air outlet of the evacuation member 110 can continuously output air flow, thereby creating a positive pressure within the slide tip 300 at any time.

Specifically, in the present embodiment, the first pumping line 120, the second pumping line 160 and the third exhaust line 180 are partially overlapped at one end close to the vacuum pumping member 110.

That is, the first pumping line 120, the second pumping line 160 and the third exhaust line 180 share a portion of the pipeline and are communicated with the pumping end of the vacuum pumping device 110 through the same interface. Therefore, the pipeline of the shared part can be used for gas flowing through different pipelines to pass through, so that the pipeline structure can be effectively simplified to facilitate layout, and the size of the sample detection device is further reduced.

The sample testing device and the pumping mechanism 100 thereof open the first valve 122, close the second valve 123 and the third valve 131, and activate the vacuum-pumping element 110, so as to form a negative pressure in the first negative pressure chamber 121. When sample enrichment is required, negative pressure can be created in the concentrator 200 by opening the second valve 123 to connect the first negative pressure chamber 121 to the concentrator 200. After the enrichment is completed, the second valve 123 is closed and the third valve 131 is opened, so that the air pressure in the enricher 200 can be recovered to be normal to perform the spotting smoothly. Because the first negative pressure chamber 121 can form and maintain negative pressure in advance, when sample enrichment is carried out, negative pressure can be formed in the enricher 200 instantly only by opening the second valve 123, and waiting time is obviously reduced. Therefore, the time required for sample enrichment is reduced, and the efficiency of sample detection is improved.

The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

The above-mentioned embodiments only represent some embodiments of the present invention, and the description thereof is specific and detailed, but not to be construed as limiting the scope of the present invention. It should be noted that, for those skilled in the art, without departing from the spirit of the present invention, several variations and modifications can be made, which are within the scope of the present invention. Therefore, the protection scope of the present invention should be subject to the appended claims.

Claims

1. A suction mechanism for evacuating a concentrator, the suction mechanism comprising:

vacuumizing the part;

2. The pumping mechanism of claim 1, further comprising a waste recovery line, the waste recovery line comprising a waste container, a suction pump, and a fourth valve, the suction pump having an input end in communication with the first negative pressure chamber and an output end in communication with the waste container, the fourth valve being disposed between the suction pump and the first negative pressure chamber.

3. The pumping mechanism as claimed in claim 2, wherein a recycling branch extends from a region of the waste liquid recycling line between the fourth valve and the suction pump, and a fifth valve is disposed on the recycling branch.

4. The aspiration mechanism of claim 1, further comprising a second aspiration line communicating the aspiration end of the evacuation member with the slide tip, the second aspiration line including a second negative pressure chamber, a sixth valve disposed between the second negative pressure chamber and the evacuation member, and a seventh valve disposed between the second negative pressure chamber and the slide tip.

5. The aspiration mechanism of claim 4, further comprising a second vent line having one end in communication with the slide tip, wherein an eighth valve is disposed on the second vent line.

6. The pumping mechanism as claimed in claim 5, wherein the eighth valve is a three-way valve, and the eighth valve has a gas inlet and two gas outlets, the gas inlet is connected to the gas outlet of the vacuum pumping device, and the two gas outlets are connected to the atmosphere and the second exhaust line, respectively.

7. The pumping mechanism as claimed in claim 6, further comprising a third exhaust line connecting the pumping end of the vacuum pumping member with the atmosphere, wherein a ninth valve is disposed on the third exhaust line.

8. The pumping mechanism of claim 7, wherein the first pumping line, the second pumping line, and the third exhaust line partially overlap at an end proximate the vacuum pumping member.

9. The aspiration mechanism of claim 4, wherein the first negative pressure chamber and the second negative pressure chamber are each provided with a pressure sensor.

10. A sample testing device, comprising:

a suction mechanism as claimed in any one of claims 1 to 9;