CN214472788U - Color comparator for determining concentration of monochromatic solution - Google Patents

Abstract

The utility model provides a color comparator for measuring the concentration of a monochromatic solution, which comprises a sample bearing part; a light emitting assembly; an image acquisition component; a dark box; a colorimetric module; the image acquisition assembly acquires a color image of the sample to be detected under the irradiation of the light emitting assembly so as to be used for the colorimetric module to determine the concentration in a colorimetric manner. The concentration of the sample to be measured is obtained by collecting the color image of the sample to be measured, extracting the color depth characteristic value of the color image and according to the relation between the color depth characteristic value and the solution concentration, the operation is simple, the concentration measuring method can be applied to the concentration measurement of various types of monochromatic solutions, and the application range is wide. The sample bearing part can be used for simultaneously placing a plurality of samples to be tested as required, and after corresponding color images are collected, the characteristic values of the color depth of the corresponding images are sequentially extracted and matched to obtain corresponding concentration values, so that high-flux quantitative analysis can be realized, and the speed is high.

Description

Color comparator for determining concentration of monochromatic solution

Technical Field

The utility model relates to a detect technical field, especially relate to a color comparator for determining monochromatic solution concentration.

Background

Colorimetric analysis is a method of determining the concentration of a substance to be measured in a colored solution by observing with the eye (or by visual colorimeter), comparing the color depth of the colored solution, or by measuring with an electro-optical colorimeter, using the color of the colored solution itself to be measured, or the color developed after adding a reagent. At present, the commonly used principle of a spectrophotometer is adopted for colorimetric analysis, a laser emitter and a laser receiver are arranged on two sides of a container for containing a sample to be detected, and the concentration of the sample to be detected is analyzed by measuring the absorbance of colored solution at a specific wavelength or within a certain wavelength range. The method can only adopt single-item sequential operation, the structure can only carry out point measurement and cannot carry out space measurement, and when the number of samples to be detected is large, a transmission structure needs to be added to transport different samples to be detected to a detection position, so the method is not suitable for occasions needing high-throughput detection.

SUMMERY OF THE UTILITY MODEL

In order to overcome the not enough of prior art, the utility model provides a color comparator body, easy operation, detection are swift, and the range of application is wide, can realize high flux and detect.

In order to achieve the above purpose, the utility model is realized by the following technical scheme.

The utility model provides a color comparator for determining monochromatic solution concentration, including the color comparator body, the color comparator body includes:

the sample bearing part is used for placing a sample to be tested which needs to be subjected to solution concentration measurement and is in a single color;

the light-emitting component is used for irradiating a sample to be detected so as to enable the sample to be detected to present a color;

the image acquisition assembly is used for acquiring a color image of a sample to be detected so as to present the color depth condition of the sample to be detected;

the camera bellows is used for providing a dark image acquisition environment;

the colorimetric module is used for extracting the characteristic value of the color depth of the color image so as to match the concentration of the sample to be detected and sending the concentration result to the display end;

the light-emitting assembly and the image acquisition assembly are respectively positioned on two sides of the sample bearing part; the image acquisition assembly acquires a color image of the sample to be detected under the irradiation of the light emitting assembly so as to be used for the colorimetric module to determine the concentration in a colorimetric manner.

Preferably, the sample bearing part is provided with a plurality of sample positions for accommodating the sample to be tested.

Preferably, the sample bearing part is detachably connected with at least one sample rack; the sample position used for containing the sample to be detected is arranged on the sample rack.

Preferably, the sample site is integrally formed with a colorless transparent container;

or, the sample site is in the shape of a hole for receiving a container.

Preferably, the dark box is provided with two openings which are respectively connected with the sample bearing part and the image acquisition assembly to form an imaging channel.

Preferably, a glass plate is arranged on one side of the sample bearing part facing the dark box.

Preferably, the sample support is located at the top of the dark box; the light-emitting component is positioned on the top of the sample bearing part; the image acquisition assembly is positioned on one side of the dark box; and a reflection assembly is arranged in the dark box to reflect the light irradiated by the light emitting assembly from the front surface of the sample to be detected to the image acquisition assembly.

Preferably, the color comparator body further comprises a housing to form a housing structure; the shell is provided with a turnover cover, and the inner wall of the turnover cover is connected with the light-emitting component; the flip moves in conjunction with the light emitting assembly to open or close the sample support.

Preferably, the housing is provided with a lid opening button; a first lock catch is arranged on the inner side of the cover opening button, and a second lock catch is arranged on the bottom plate of the flip cover; the cover opening button is matched with the second lock catch to lock or unlock the flip cover.

Preferably, the color depth characterization value comprises R, G, B three-channel component matching results of each pixel point of the color image under the RGB model;

or, the color depth characterization value comprises a matching result of brightness values and saturation values of all pixel points of the color image under the HSV/HSB model.

Compared with the prior art, the beneficial effects of the utility model reside in that:

the utility model provides a pair of a color comparator for determining monochromatic solution concentration, through the color image of gathering the sample that awaits measuring, to the color depth value of color image draw, obtain the concentration of the sample that awaits measuring according to the relational expression between color depth value and the solution concentration, easy operation, and can use the determination of the monochromatic solution concentration of multiple different grade type, the range of application is wide. The sample bearing part can be used for simultaneously placing a plurality of samples to be tested as required, and after corresponding color images are collected, the characteristic values of the color depth of the corresponding images are sequentially extracted and matched to obtain corresponding concentration values, so that high-flux quantitative analysis can be realized, and the speed is high.

The above description is only an overview of the technical solution of the present invention, and in order to make the technical means of the present invention clearer and can be implemented according to the content of the specification, the following detailed description is made with reference to the preferred embodiments of the present invention and accompanying drawings. The detailed description of the present invention is given by the following examples and the accompanying drawings.

Drawings

The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the invention and together with the description serve to explain the invention without undue limitation to the invention. In the drawings:

fig. 1 is a structural sectional view of a color comparator body of the present invention;

FIG. 2 is a schematic view of an assembly structure of the sample rack and the container according to the present invention;

FIG. 3 is an enlarged view of FIG. 1 at A;

fig. 4 is an assembly structure diagram of the travel switch of the present invention;

fig. 5 is a schematic view of a three-dimensional structure of the color comparator body according to the present invention;

fig. 6 is a schematic view of a three-dimensional structure of the color comparator body of the present invention;

FIG. 7 is a graph of a linear regression analysis of a concentration calculation formula according to the present invention;

fig. 8 is a linear regression analysis diagram of another concentration calculation formula according to the present invention;

FIG. 9 is a graph of a linear regression analysis of another concentration calculation formula of the present invention;

fig. 10 is a linear regression analysis diagram of another concentration calculation formula according to the present invention.

In the figure: 1. a colorimeter body;

10. a sample-bearing portion; 11. a sample rack; 12. a container; 13. a glass plate;

20. a light emitting assembly; 21. a fixing plate; 22. a lamp panel; 23. a light box;

30. an image acquisition component;

40. a dark box;

50. a reflective component; 51. a mirror; 52. a fixing plate; 53. an adjusting frame;

60. a housing; 61. a cover is turned; 611. a base plate; 6111. a second lock catch; 62. a flip button; 621. a first lock catch; 63. a switch; 64. a power button;

70. a gas strut;

80. a travel switch.

Detailed Description

The foregoing and other objects, features, aspects and advantages of the present invention will become more apparent to those skilled in the art from the following detailed description, which, taken in conjunction with the annexed drawings, discloses a more detailed description of the present invention, which will enable those skilled in the art to make and use the present invention. In the drawings, the shape and size may be exaggerated for clarity, and the same reference numerals will be used throughout the drawings to designate the same or similar components. In the following description, terms such as center, thickness, height, length, front, back, rear, left, right, top, bottom, upper, lower, and the like are used based on the orientation or positional relationship shown in the drawings. In particular, "height" corresponds to the dimension from top to bottom, "width" corresponds to the dimension from left to right, and "depth" corresponds to the dimension from front to back. These relative terms are for convenience of description and are not generally intended to require a particular orientation. Terms concerning attachments, coupling and the like (e.g., "connected" and "attached") refer to a relationship wherein structures are secured or attached, either directly or indirectly, to one another through intervening structures, as well as both movable or rigid attachments or relationships, unless expressly described otherwise.

The present invention will be further described with reference to the accompanying drawings and the detailed description, and it should be noted that the embodiments or technical features described below can be arbitrarily combined to form a new embodiment without conflict.

Example 1

The utility model provides a color comparator for determining monochromatic solution concentration, including color comparator body 1, as shown in figure 1, this 1 includes of color comparator:

a sample bearing part 10 for placing a sample to be measured which is required to be subjected to solution concentration measurement and is in a single color; specifically, the sample to be tested is a monochromatic solution to be tested, and the prepared sample to be tested is placed on the sample bearing part 10 before the concentration is measured;

a light emitting device 20 for illuminating a sample to be tested to make the sample to be tested show a color; specifically, the light emitting assembly 20 emits light from a side of the sample to be detected, which faces away from the image collecting assembly 30, to irradiate the sample to be detected, so that the sample to be detected presents a color to provide light required by the image collecting assembly 30 to collect an image; in one embodiment, the light emitting assembly 20 includes a plurality of LED beads, which are uniformly distributed and arranged toward the sample supporting portion 10 to emit uniform light to the sample supporting portion 10;

the image acquisition component 30 is used for acquiring a color image of a sample to be detected so as to present the color shade condition of the sample to be detected; specifically, the image collecting assembly 30 has an image generating capability, images the sample to be measured at an imaging position and generates a corresponding color image, for the same type of monochromatic solution, the concentrations are different, the color type of the collected corresponding color image is not changed, and the color depth of the color image is changed; further, image acquisition assembly 30 includes, but is not limited to, a camera, a video camera;

a dark box 40 for providing a dark image capturing environment; the intensity of the light in the camera bellows 40 when the light emitting component 20 emits the light is controlled by the matching of the light emitting component 20 and the camera bellows 40; in addition, a dark environment inside the dark box 40 is ensured when the light emitting assembly 20 does not emit light;

the colorimetric module is used for extracting the characteristic value of the color depth of the color image so as to match the concentration of the sample to be detected and sending the concentration result to the display end; specifically, the light is an electromagnetic wave, and is naturally a mixed light composed of electromagnetic waves with different wavelengths (380-780 nm) according to a certain proportion, and can be decomposed into continuous visible spectrums with various colors such as red, orange, yellow, green, cyan, blue, purple and the like through a prism; when white light passes through the solution, if the solution does not absorb light of various wavelengths, the solution is colorless; if the solution absorbs a part of the light with the wavelength, the solution takes on the color of the light which is remained after the solution is transmitted; the color of the colored solution is complementary to the color of the absorbed light; the more absorption, the darker the complementary color; comparing the color depth of the colored solution, namely comparing the absorption degree of the colored solution to the light absorbed by the colored solution, namely obtaining the concentration of the sample to be detected by comparing the color depth of the color image;

the light emitting assembly 20 and the image collecting assembly 30 are respectively positioned at two sides of the sample bearing part 10; the image collecting assembly 30 collects a color image of the sample to be measured under the irradiation of the light emitting assembly 20, so that the colorimetric module can measure the concentration in a colorimetric manner. The image acquisition assembly 30 and the light-emitting assembly 20 are respectively arranged at two sides of a sample to be measured, when the colorimeter body 1 is started for concentration measurement, the light-emitting assembly 20 emits light rays which irradiate towards the sample to be measured, and the light rays are focused on an imaging plane of the image acquisition assembly 30 after being linearly transmitted, refracted or reflected, so that an image of the sample to be measured is finally obtained; further, as the image collecting area, a local position image of the sample to be measured or an image of the entire sample to be measured or an image including the container 12 containing the sample to be measured may be used as necessary. And the colorimetric module extracts color depth data according to the color image to obtain a color depth characteristic value, and the concentration of the sample to be measured is calculated through conversion according to a relational expression between the color depth characteristic value and the concentration.

It will be understood that the color of a solution is determined by the solute, and that a solution that is monochromatic means that the color of the solution is single, and that for the same solution, the darker the color of the solution, the greater the concentration. It should be understood that when the method of the present application is used for measuring the concentration of a monochromatic solution, when a plurality of substances affecting the color of the monochromatic solution exist in the monochromatic solution, only the measured substance in the monochromatic solution can be colored by the pretreatment, and any existing pretreatment method can be adopted.

In an embodiment, in order to improve the measurement efficiency of the color comparator body 1, the sample bearing part 10 is provided with a plurality of sample positions for accommodating the samples to be measured, so that images can be collected on a plurality of samples to be measured simultaneously, and the color comparison module sequentially analyzes a plurality of color images to obtain the concentration of the corresponding samples to be measured, thereby realizing high-throughput quantitative analysis and being simple and rapid in operation.

Further, the sample support 10 is detachably connected with at least one sample rack 11; the sample position for accommodating the sample to be tested is arranged on the sample rack 11. When the measured sample needs to be disposed of after the concentration measurement is finished, the sample rack 11 is taken down for processing, and the sample bearing part 10 is convenient and not easy to be polluted due to improper operation.

Further, as shown in fig. 1 and fig. 2, the sample site is integrally formed with a colorless transparent container 12; when the measured sample needs to be processed, the sample rack 11 is taken down, the measured sample is processed, and the container 12 on the sample position is cleaned for the next concentration measurement;

or, the sample site is in the form of a well for receiving the container 12; when the measured sample needs to be processed, the sample rack 11 is taken down, the container 12 is taken down to process the measured sample, and the container 12 is cleaned, so that the operation is convenient, and the sample rack 11 is not easy to be polluted. When a sample to be tested needs to be placed, the sample to be tested is placed into the container 12, and then the container 12 is placed into the corresponding sample position, so that the operation is simple, convenient and quick. It should be understood that the container 12 is transparent and colorless so as not to affect the color status of the sample to be tested.

In one embodiment, as shown in FIG. 1, the dark box 40 has two openings, which are connected to the sample-holding portion 10 and the image capturing assembly 30, respectively, to form an imaging channel. Sample bearing part 10 orientation camera bellows 40 one side is equipped with glass board 13, and glass board 13 is colorless transparent structure to separate sample bearing part 10 and camera bellows 40 internal cavity, in order to avoid the sample that awaits measuring to cause the pollution of camera bellows 40 cavity, and make light-emitting component 20 from the sample that awaits measuring back to the light that image acquisition component 30 back sent jet into camera bellows 40 in the box through glass board 13, and convey to image acquisition component 30 for image acquisition.

Further, the sample support 10 is located on top of the dark box 40; the light emitting assembly 20 is positioned on top of the sample support 10; the image acquisition assembly 30 is positioned at one side of the camera bellows 40; a reflection assembly 50 is disposed in the dark box 40 to reflect the light emitted from the front surface of the sample to be measured by the light emitting assembly 20 toward the image collecting assembly 30. Specifically, since the sample to be measured is liquid, in order to facilitate the collection of the color image of the sample to be measured, the container 12 for containing the sample to be measured is open; in order to prevent the sample to be tested from falling, the container 12 is opened upwards; in order to reduce the influence of the outer wall of the container 12 on the image acquisition of the sample to be measured, the light emitting assembly 20 is located at the top of the sample bearing part 10, that is, the light emitting assembly 20 is located at the open top of the container 12, so that the image acquisition assembly 30 acquires the image of the sample to be measured in the open top of the container 12. In addition, the image capturing assembly 30 is located at one side of the dark box 40, and the reflection assembly 50 enables the image capturing assembly 30 to successfully capture a color image of the sample to be measured. The reasonable layout of the installation positions of the sample bearing part 10, the light-emitting component 20 and the image acquisition component 30 is beneficial to the miniaturization design of the color comparator body 1.

Further, the reflection assembly 50 includes a reflection mirror 51, a fixing frame 52, and an adjusting frame 53, wherein the reflection mirror 51 is fixed in the groove of the fixing frame 52, the back of the fixing frame 52 is connected to the adjusting frame 53, and the adjusting frame 53 is used for adjusting the inclination angle of the reflection mirror 51 to adjust the light intensity when the image acquisition assembly 30 acquires the image. Further, the adjusting frame 53 is electrically connected to the control board, and the control board controls the adjusting frame 53 to move by manually inputting an instruction, so as to adjust the tilting angle of the reflector 51.

In one embodiment, the light emitting assembly 20 includes a fixing plate 21, and a plurality of LED beads are disposed on a side of the fixing plate 21 facing the sample holder 10 to emit light. Further, the light emitting assembly 20 further includes at least one lamp panel 22 to improve the light intensity. Further, the light emitting assembly 20 includes a lamp housing 23 for fixing the fixing plate 21 and the lamp panel 22; the lamp box 23 is a shell structure, one side of the lamp box 23 facing the sample bearing part 10 is open, and the inner side wall of the lamp box 23 is placed around the sample bearing part 10, so that the light emitted by the light emitting component 20 is emitted to the sample bearing part 10.

In one embodiment, as shown in fig. 1, the color comparator body 1 further includes a housing 60 to form a housing structure; the shell 60 is provided with a flip cover 61, and the inner wall of the flip cover 61 is connected with the light-emitting component 20; the flip 61 moves in conjunction with the light emitting assembly 20 to open or close the sample support 10. The housing 60 is provided to accommodate the sample holder 10, the light emitting module 20, the image capturing module 30, the camera bellows 40, and the colorimetric module, and to protect the internal components and prevent dust. Further, the color comparator body 1 is provided with an air stay bar 70, the air stay bar 70 is connected with the flip cover 61, and the flip cover 61 is automatically opened or closed through the air stay bar 70. Further, the sample-supporting part 10 and the light-emitting component 20 are disposed close to the inner wall of one side of the housing 60, so as to reasonably arrange the space inside the housing 60 occupied by the imaging channel formed by the sample-supporting part 10, the light-emitting component 20 and the image capturing component 30. Further, the bottom wall of the casing 60 forms the bottom wall structure of the camera chamber 40, so as to increase the size of the internal cavity space of the camera chamber 40 and facilitate the miniaturization design of the casing 60.

Further, as shown in fig. 1, 3 and 6, the housing 60 is provided with a door opening button 62; the inner side of the cover opening button 62 is provided with a first lock 621, and the bottom plate 611 of the flip cover 61 is provided with a second lock 6111; the cover opening button 62 is matched with the second lock catch 6111 to lock or unlock the flip cover 61. When the flip cover 61 is needed, the cover opening button 62 is pressed, the first lock 621 is separated from the second lock 6111, and the flip cover 61 can be opened; when the flip cover 61 needs to be closed, the flip cover 61 is closed and the flip cover 61 is pressed down, so that the first lock 621 and the second lock 6111 are formed, and the closed and tight closing of the flip cover 61 can be ensured.

Further, as shown in fig. 1, the flip cover 61 is connected with an air stay 70, and in one embodiment, the air stay 70 is controlled to open the flip cover 61 or pull the flip cover 61 by inputting a command manually. Further, when the lid opening button 62 is pressed, the control board sends a command input to the gas strut 70 to open the lid 61 for a few seconds or a fixed time, or to automatically open or close the lid through the display screen. In still another embodiment, as shown in fig. 4, the color comparator body 1 is provided with a travel switch 80, a contact of the travel switch 80 faces an outer wall of one side of the flip 61, when the flip 61 is closed, the contact of the travel switch 80 contacts with the outer wall of the flip 61, when the flip 61 is opened, the outer wall of the flip 61 moves out of contact with the contact of the travel switch 80, and the travel switch 80 sends a signal to instruct the flip 61 to stop moving, thereby controlling the flip angle of the flip 61.

In one embodiment, as shown in fig. 1 and 6, the housing 60 is provided with a switch 63 for turning on or off the concentration measurement. As shown in fig. 5, the housing 60 is further provided with a power interface 64 for supplying power to the electrical components within the housing 60. The housing 60 is further provided with an output port for outputting the working state or concentration result of the electrical component to be monitored in the housing 60 to a display end for a user to check; or, the housing 60 is provided with a display end for the user to view the working state or concentration result of the electrical components to be monitored in the housing 60.

In one embodiment, as shown in fig. 1, 5 and 6, the bottom of the casing 60 is provided with a plurality of foot pads 65 to improve the stability of the casing 60 when it is placed. Further, the foot pad 65 is a rubber member to improve the cushioning performance of the foot pad 65.

In one embodiment, the color depth characterization value includes R, G, B three-channel component matching results of each pixel point of the color image under the RGB model. Specifically, R, G, B represent a red R color channel, a green G color channel, and a blue B color channel, respectively. And obtaining an R mean value, a G mean value and a B mean value of the image under the RGB model according to the R, G, B three-channel components of each pixel point to obtain a color depth characterization value of the image. Substituting the obtained three-channel components of the R mean value, the G mean value and the B mean value into a concentration calculation formula

Wherein A, C is a coefficient related to a specific sample to be measured, e is a constant, τ is concentration, and the unit of concentration is g/L. Alternatively, the obtained R, G, and B mean values are substituted into a concentration calculation formula τ ═ K × [ Max (R, G, B) -Min (R, G, B)]+C₁(ii) a Wherein tau is the concentration of the monochromatic solution, K is a coefficient related to a sample to be measured, C₁To adjust the coefficients.

Specifically, the concentration calculation formula stored in the colorimetric module is

And when the solution type matching library is stored in the colorimetric module. The establishment of the solution type matching library comprises the following steps: acquiring color images of a plurality of known types of standard solutions; obtaining an R mean value, a G mean value and a B mean value of corresponding color images; and according to the obtained R mean value, G mean value and B mean value and the concentration of the corresponding standard solution, obtaining A, C coefficient values after corresponding to a concentration calculation formula to obtain a solution type matching library. When the color comparator body 1 is used for measuring the concentration of a plurality of different types of samples, the type of the sample to be measured is input, and the sample is in the solution typeThe corresponding A, C coefficient value is matched in the type matching library, and then a specific concentration calculation formula is obtained, so that the detection can be carried out, the efficiency is high, the speed is high, the application range is wide, and the solution type matching library can be updated at any time so as to expand the application objects of the color comparator body 1. It should be understood that when determining A, C coefficient values for the same type of standard solution, the standard solution is configured into at least two samples of different concentrations to calculate A, C coefficient values, since there are two coefficient values.

When the concentration calculation formula stored by the colorimetric module is tau-K₁×[Max(R,G,B)-Min(R,G,B)]+C₁When, K₁、C₁The coefficient value can also be obtained by the above concentration calculation formula as

The method for obtaining the corresponding coefficient value is not described herein again.

In another embodiment, the color depth characterization value includes a matching result of lightness values and saturation values of each pixel point of the color image under the HSV/HSB model. In the HSV color space of the image, H is a chromatic value, and the color is determined; s is a saturation value, namely the color depth; v is lightness, i.e., brightness. According to the colors corresponding to the absorption degrees of the light to the liquids with different concentrations, the chroma and the concentration of the colored solution of the same substance are irrelevant, the saturation value, the brightness value and the concentration are relevant, the higher the concentration is, the higher the saturation value of the image is, the lower the brightness value is, namely, the saturation value is positively correlated with the concentration, and the brightness value is negatively correlated with the concentration. The saturation value S represents the degree to which the color approaches the spectral color; a color can be considered as a result of mixing a certain spectral color with white, wherein the greater the proportion of the spectral color, the higher the degree of the color approaching the spectral color, and the higher the saturation value of the color; the saturation value is high, and the color is dark and bright; the white light component of the spectrum color is 0, and the saturation value reaches the highest value; the value range is usually 0% -100%, and the larger the value is, the more saturated the color is; the brightness value represents the brightness degree of the color, and for the light source color, the brightness value is related to the brightness of the luminous body; for object colors, this value is related to the transmittance or reflectance of the object, and typically ranges from 0% (black) to 100% (white). The lightness value represents the brightness of a pixel point of the collected image, the saturation value represents the color depth, the color type is determined for the same colored solution, and the brightness and the depth of the color can change according to the concentration change of the colored solution; namely, the brightness value and the saturation value of the color image of the sample to be detected have corresponding relations with the concentration of the sample to be detected. The brightness value and the saturation value of the color image represent the color depth characteristic value of the corresponding sample to be detected, and for the same type of sample, the sample concentration changes, and the color characteristic value of the color image also changes.

Further, the colorimetric module calculates a brightness mean value V and a saturation mean value S corresponding to the brightness value and the saturation value of each pixel point of the image under the HSV/HSB model to indicate the concentration of the solution to be detected.

Further, the colorimetric module constructs an HSV model according to the R, G, B three-channel value of each pixel point of the color image under the RGB model and the R, G, B three-channel value so as to calculate the brightness value and the saturation value of each pixel point of the image under the HSV/HSB model. Further, in an embodiment, the colorimetric module obtains the brightness value and the saturation value of each pixel according to the R, G, B three-channel value of each pixel in the target region of the image. In another embodiment, when the difference between the shades of any two pixels in the monochromatic solution is smaller than the preset threshold, the colorimetric module obtains the brightness value and the saturation value of the corresponding pixel according to the R, G, B three-channel values of a preset number of pixels in the target region of the image. Specifically, when the color depth difference of each part of the solution to be detected is not large, the difference between three channel values of different pixel points of the obtained image is not large, and three channel values of all pixel points in the target area do not need to be obtained, that is, the color depth difference of any two pixel points is smaller than a preset threshold value, R, G, B three channel values of a preset number of pixel points are selected. Specifically, the image acquisition device acquires an image of a sample rack placed with a hemoglobin solution to be detected, records the image as an original image, and divides the original image, wherein one hemoglobin solution to be detected is an image and is recorded as a first image; and dividing a target area of the first image, and acquiring R, G, B three-channel values of all pixel points or a preset number of pixel points in the target area, thereby acquiring a brightness value and a saturation value of the first image.

Further, the color comparison module substitutes the lightness mean value V and the saturation mean value S into a concentration calculation formula

Wherein V is lightness average, S is saturation average, A₂、C₂And e is a constant and tau is the concentration, which is the coefficient related to the specific sample to be detected. Specifically, the coefficient A is known according to the type of the sample to be measured₂、C₂And substituting the acquired V value and S value into a concentration calculation formula, and further solving a concentration value.

Or the colorimetric module substitutes the saturation mean value S and the lightness mean value V into a concentration calculation formula tau ═ K₃×S×V+C₃(ii) a Wherein, tau is concentration, K₃As a coefficient related to the sample to be measured, C₃To adjust the coefficients.

Or substituting the saturation mean value S and the lightness mean value V into a concentration calculation formula by the colorimetric module

Wherein, tau is the concentration of the monochromatic solution, A₄、B₄E is a constant, which is a coefficient related to the sample to be measured.

Or substituting the saturation mean value S and the lightness mean value V into a concentration calculation formula by the colorimetric module

Wherein, tau is the concentration of the monochromatic solution, A₅、B₅Is a coefficient related to the sample to be measured.

Specifically, when the concentration is calculated by the formula

And when the solution type matching library is stored in the colorimetric module. The establishment of the solution type matching library comprises the following steps: acquiring color images of a plurality of known types of standard solutions; obtainingA saturation mean S and a brightness mean V of the corresponding color images; substituting the concentration of the corresponding standard solution, the corresponding saturation average S and the corresponding brightness average V into a concentration calculation formula to obtain a coefficient value; that is, the corresponding sample concentration, the lightness average value and the saturation average value of the color image are substituted into the concentration calculation formula to determine the coefficient value A corresponding to the sample of the corresponding type₂、C₂. It will be understood that for the same type of standard solution, A is determined₂、C₂When the coefficient value is two, the standard solution is configured into at least two samples with different concentrations to calculate A₂、C₂Coefficient values.

It should be understood that when the concentration calculation formula is τ ═ K₃×S×V+C₃Or

Or

When determining the coefficient value of the concentration calculation formula, the concentration calculation formula can be adopted as

The method for obtaining the corresponding coefficient value is realized, and the details are not repeated herein.

The four concentration calculation formulas are described in detail below.

When the concentration is calculated by the formula

Specifically, 16 sets of samples of known concentration were prepared for linear regression analysis. Corresponding color images are respectively acquired to determine A, C coefficient values according to the relationship between the three-channel component matching results of the images of the samples and the sample concentrations. Groups 1 to 16 become progressively darker in color. Obtaining R, G, B three-channel components of corresponding color image, calculating Max (R, G, B) and Min (R, G, B), further calculating the ratio of the square of Max (R, G, B) and the difference between Max (R, G, B) and Min (R, G, B) as the color image of corresponding sampleColor characterization results; wherein R, G, B in Max (R, G, B) and Min (R, G, B) respectively represent R mean, G mean and B mean. As shown in fig. 7, a relational graph is formed by using the color characterization results and the densities of the color images of the 16 groups of samples as an abscissa and an ordinate, respectively; the abscissa x represents the ratio of the square of Max (R, G, B) to the difference between Max (R, G, B) and Min (R, G, B), y represents the concentration, and a relational expression between x and y is obtained as 0.5222e^-1.001x(ii) a Wherein the factor a is equal to 0.5222; c is equal to-1.001; r²The correlation index is expressed to reflect the effect of linear regression analysis, the correlation index is between 0 and 1, the closer to 1, the better the regression fitting effect is, and generally, the model fitting goodness of more than 0.8 is considered to be higher. Further determining a new and convenient concentration calculation formula

The concentration of a single sample can be measured, and the concentration of a plurality of samples can be measured simultaneously, so that high-flux quantitative analysis is realized, and the method is rapid and convenient.

The corresponding relation between the color characterization results and the concentration values of the 16 groups of samples is shown in a table I.

Watch 1

When the concentration calculation formula is tau ═ K₁×[Max(R,G,B)-Min(R,G,B)]+C₁Specifically, 17 sets of samples of known concentration were prepared for linear regression analysis. The corresponding color images are respectively obtained, and the colors of the groups 17 to 33 are gradually darkened to be different according to the difference value between Max (R, G, B) and Min (R, G, B) of the color images (namely [ Max (R, G, B) -Min (R, G, B)]) Determination of K in relation to the concentration of the corresponding sample₁Numerical value, C₁A numerical value; wherein R, G, B in Max (R, G, B) and Min (R, G, B) respectively represent R mean, G mean and B mean. After the color image of the corresponding sample is obtained, R, G, B three-channel components of the color image are obtained to obtain Max (R, G, B) and Min (R, G, B), and the difference between Max (R, G, B) and Min (R, G, B) is calculated. As shown in the figure8, [ Max (R, G, B) -Min (R, G, B) in 17 sets of samples]The numerical value and the concentration of (A) are respectively a relational graph formed by an abscissa and an ordinate. The abscissa x represents [ Max (R, G, B) -Min (R, G, B)]The value of (d); the ordinate y represents concentration; obtaining a relation between x and y, wherein y is-0.0053 x + 0.7895; wherein the coefficient K₁Equal to-0.0053; coefficient C₁Equal to 0.7895; r²The correlation index is expressed to reflect the effect of linear regression analysis, the correlation index is between 0 and 1, the closer to 1, the better the regression fitting effect is, and generally, the model fitting goodness of more than 0.8 is considered to be higher. Further, the concentrations τ and [ Max (R, G, B) -Min (R, G, B)]The relation between the concentration and the concentration is obtained, and a new and convenient concentration calculation formula tau is obtained₁×[Max(R,G,B)-Min(R,G,B)]+C₁. The concentration of a single sample to be detected can be measured, and the concentration of a plurality of samples to be detected can be measured simultaneously, so that high-flux quantitative analysis is realized, and the method is quick and convenient.

The corresponding relationship between the numerical values and concentration values of [ Max (R, G, B) -Min (R, G, B) ] of 17 groups of samples is shown in a table II. Wherein, "/255" in the second table represents the color value unit of the image under the RGB model.

Watch two

Serial number	Concentration (g/L)	[Max(R,G,B)-Min(R,G,B)]/255
			Group 17	0.5	51.32802
Group 18	0.48	65.15146
			Group 19	0.46	63.26312
Group 20	0.45	70.03295
			Group 21	0.43	70.51928
Group 22	0.41	72.21342
			Group 23	0.4	71.79225
Group 24	0.38	75.13528
			Group 25	0.36	80.2585
Group 26	0.35	87.13145
			Group 27	0.33	80.52519
Group 28	0.3	89.05224
			Group 29	0.26	95.94428
Group 30	0.23	111.1566
			Group 31	0.2	111.3673
Group 32	0.16	121.0743
			Group 33	0.13	123.2462

When the concentration is calculated by the formula

Specifically, 17 sets of samples of known concentration were prepared for linear regression analysis. Respectively acquiring corresponding color images, and gradually deepening the colors of the groups 34 to 50 to determine A according to the relationship between the color characterization result of the color image of the sample and the concentration of the monochromatic solution₂、C₂Coefficient values. And after the color image of the corresponding sample is obtained, the brightness value and the saturation value of the color image are obtained, and a color characterization result (brightness value: saturation value) is calculated. As shown in fig. 9, the results of color characterization and the concentrations of 17 groups of samples are plotted on the abscissa and the ordinate, respectively, to form a relationship graph. The abscissa x representsThe ratio of the lightness mean value to the saturation mean value, wherein the lightness mean value is a numerator, and the saturation mean value is a denominator; the ordinate y represents concentration; obtaining the relation between x and y, wherein y is 0.7384e^-1.79x(ii) a Wherein the coefficient A₂Equal to 0.7384; coefficient C₂Equal to-1.79; r²The correlation index is expressed to reflect the effect of linear regression analysis, the correlation index is between 0 and 1, the closer to 1, the better the regression fitting effect is, and generally, the model fitting goodness of more than 0.8 is considered to be higher. Further determining a new and convenient concentration calculation formula

The concentration of a single sample to be detected can be measured, and the concentration of a plurality of samples to be detected can be measured simultaneously, so that high-flux quantitative analysis is realized, and the method is quick and convenient.

The corresponding relation between the color characterization result and the concentration value of 17 groups of samples is shown in the third table.

Watch III

Serial number	Concentration g/L	V/S
			Group 34	0.5	0.233476
Group 35	0.48	0.303853
			Group 36	0.46	0.293588
Group 37	0.45	0.330708
			Group 38	0.43	0.329197
Group 39	0.41	0.34124
			Group 40	0.4	0.340304
Group 41	0.38	0.358868
			Group 42	0.36	0.388601
Group 43	0.35	0.430578
			Group 44	0.33	0.390709
Group 45	0.3	0.448241
			Group 46	0.26	0.506873
Group 47	0.23	0.666133
			Group 48	0.2	0.657731
Group 49	0.16	0.837074
			Group 50	0.13	1.046687

When the concentration calculation formula is tau ═ K₃×S×V+C₃Specifically, 17 sets of samples of known concentration were prepared for linear regression analysis. Respectively acquiring corresponding color images, wherein the colors of the groups 51 to 67 are gradually deepened to determine K of corresponding concentration calculation formula according to the relation between the product value of lightness mean and saturation mean of the color image of the sample and the concentration of the sample₃Numerical value, C₃Numerical values. After the color image of the corresponding monochromatic solution is obtained, the lightness mean value and the saturation mean value of the color image are obtained, and the product value of the lightness mean value and the saturation mean value is calculated. As shown in fig. 10, the product value of the lightness mean and the saturation mean and the concentration of 17 groups of monochromatic solutions are plotted on the abscissa and the ordinate, respectively. The abscissa x represents the product of the lightness mean and the saturation mean; the ordinate y represents concentration; obtaining a relation between x and y, wherein y is-0.0053 x + 0.7895; wherein the coefficient K₃Equal to-0.0053; adjustment coefficient C₃Equal to 0.7895; r²The correlation index is expressed to reflect the effect of linear regression analysis, the correlation index is between 0 and 1, the closer to 1, the better the regression fitting effect is, and generally, the model fitting goodness of more than 0.8 is considered to be higher. Further, a new and convenient concentration calculation formula tau ═ K is obtained₃×S×V+C₃. The concentration of a single sample to be detected can be measured, and the concentration of a plurality of samples to be detected can be measured simultaneously, so that high-flux quantitative analysis is realized, and the method is quick and convenient.

The product value of lightness mean and saturation mean and the corresponding relationship of concentration value of 17 groups of samples are shown in the fourth table.

Watch four

Serial number	Concentration (g/L)	S*V
			Group
51	0.5	51.32802
			Group 52	0.48	65.15146
Group 53	0.46	63.26312
			Group 54	0.45	70.03295
Group 55	0.43	70.51928
			Group 56	0.41	72.21342
Group 57	0.4	71.79225
			Group 58	0.38	75.13528
Group 59	0.36	80.2585
			Group 60	0.35	87.13145
Group 61	0.33	80.52519
			Group 62	0.3	89.05224
Group 63	0.26	95.94428
			Group 64	0.23	111.1566
Group 65	0.2	111.3673
			Group 66	0.16	121.0743
Group 67	0.13	123.2462

It should be understood that,

and

the linear regression analysis of the two concentration calculation formulas has the same principle as the linear regression analysis method of the concentration calculation formula, and is not repeated here.

In another embodiment, the color comparator body 1 is used to detect the target concentration by performing a pre-treatment on the sample, such as detecting the glutamic-pyruvic transaminase concentration in the blood sample. Specifically, a blood sample is taken, red blood cells are removed by centrifugation, blood plasma is left, and a color reaction reagent is added to obtain a sample to be detected. Specifically, the reaction principle of the glutamic-pyruvic transaminase and the chromogenic reaction reagent is as follows: glutamate pyruvate transaminase acts on a substrate consisting of alanine and alpha-ketoglutarate at 37 ℃ and pH 7.4 to produce pyruvate and glutamate. After reacting for 30min, adding 2, 4-dinitrophenylhydrazine hydrochloric acid solution to stop the reaction, and simultaneously carrying out addition reaction on the 2, 4-dinitrophenylhydrazine and carbonyl in keto acid to generate pyruvic acid phenylhydrazone. The phenylhydrazone is reddish brown under the alkaline condition, so that the sample to be detected is obtained, and the deeper the reddish brown of the sample to be detected is, the higher the glutamic-pyruvic transaminase concentration in a blood sample corresponding to the sample to be detected is.

The utility model discloses compare prior art, 1 range of application of color comparator body of this application is wide, can convert the concentration that obtains the sample that awaits measuring through the collection to the color image of the sample that awaits measuring, the extraction of colour depth degree eigenvalue. The method is simple to operate and quick to detect, and for the types of the samples which are not stored in the color comparator body 1, the coefficient values corresponding to the corresponding concentration calculation formulas can be obtained by calibrating the standard solution of the samples, so that the types of the determination targets can be increased at any time.

The above is only a preferred embodiment of the present invention, and is not intended to limit the present invention in any way; the utility model can be smoothly implemented by the ordinary technicians in the industry according to the drawings and the above description; however, those skilled in the art should understand that changes, modifications and variations made by the above-described technology can be made without departing from the scope of the present invention, and all such changes, modifications and variations are equivalent embodiments of the present invention; meanwhile, any changes, modifications, evolutions, etc. of the above embodiments, which are equivalent to the actual techniques of the present invention, still belong to the protection scope of the technical solution of the present invention.

Claims (10)

1. A color comparator for determining the concentration of a monochromatic solution, comprising a color comparator body (1), characterized in that the color comparator body (1) comprises:

the sample bearing part (10) is used for placing a sample to be measured which needs to be subjected to solution concentration measurement and is in a single color;

the light-emitting component (20) is used for irradiating a sample to be detected so as to enable the sample to be detected to present a color;

the image acquisition assembly (30) is used for acquiring a color image of a sample to be detected so as to present the color shade condition of the sample to be detected;

a dark box (40) to provide a dark image acquisition environment;

the colorimetric module is used for extracting the characteristic value of the color depth of the color image so as to match the concentration of the sample to be detected and sending the concentration result to the display end;

the light-emitting component (20) and the image acquisition component (30) are respectively positioned at two sides of the sample bearing part (10); the image acquisition assembly (30) acquires a color image of the sample to be measured under the irradiation of the light emitting assembly (20) so as to be used for the colorimetric module to measure the concentration in a colorimetric manner.

2. The color comparator for determining the concentration of a monochromatic solution according to claim 1, wherein the sample-holding portion (10) is provided with a plurality of sample sites for receiving the samples to be measured.

3. The color comparator for determining the concentration of a monochromatic solution according to claim 1, wherein the sample-bearing section (10) has at least one sample holder (11) detachably attached thereto; the sample position used for containing the sample to be tested is arranged on the sample rack (11).

4. A colorimeter for determining the concentration of a monochromatic solution according to claim 2 or 3 characterised in that said sample station is integrally formed with a colourless transparent container (12);

or, the sample site is in the form of a well for receiving the container (12).

5. The color comparator for determining the concentration of a monochromatic solution according to claim 1, wherein the dark box (40) is provided with two openings respectively connected with the sample-bearing portion (10) and the image acquisition assembly (30) to form an imaging channel.

6. The color comparator for determining the concentration of a monochromatic solution according to claim 5, characterized in that the sample-carrying section (10) is provided with a glass plate (13) on the side facing the dark box (40).

7. The color comparator for determining the concentration of a monochromatic solution according to claim 1, characterized in that the sample-bearing portion (10) is located on top of the dark box (40); the light emitting assembly (20) is positioned on top of the sample support (10); the image acquisition assembly (30) is positioned at one side of the camera bellows (40); and a reflection assembly (50) is arranged in the dark box (40) to reflect the light irradiated by the light emitting assembly (20) from the front surface of the sample to be detected to the image acquisition assembly (30).

8. The color comparator for determining the concentration of a monochromatic solution according to claim 1, characterized in that the color comparator body (1) further comprises a case (60) for forming a housing structure; the shell (60) is provided with a flip cover (61), and the inner wall of the flip cover (61) is connected with the light-emitting component (20); the flip (61) moves in conjunction with the light emitting assembly (20) to open or close the sample support (10).

9. The color comparator for determining the concentration of a monochromatic solution according to claim 8, characterized in that the housing (60) is provided with a lid-opening button (62); a first lock catch (621) is arranged on the inner side of the cover opening button (62), and a second lock catch (6111) is arranged on the bottom plate (611) of the flip cover (61); the cover opening button (62) is matched with the second lock catch (6111) to lock or unlock the flip cover (61).

10. The colorimeter for determining the concentration of a monochromatic solution according to claim 1 wherein said color depth characterization values include R, G, B three-channel component matching results for pixel points of a color image under an RGB model;

or, the color depth characterization value comprises a matching result of brightness values and saturation values of all pixel points of the color image under the HSV/HSB model.

Images