CN214041428U - Liquid transfer mechanism in protein tracing instrument - Google Patents

Abstract

The utility model provides a pair of move liquid mechanism in protein tracing appearance, including imbibition needle, imbibition arm, cam, wherein, the imbibition needle is fixed in on the imbibition arm, the cam with the imbibition arm contact, be equipped with first rotatory fulcrum on the imbibition arm, cam motion can drive the imbibition needle winds first rotatory fulcrum rotates, the imbibition needle can be used for absorbing the waste liquid in the reaction tank, moves liquid mechanism and still includes support element, support element is the telescopic or has elasticity, support element can let the imbibition arm all the time with the cam keeps the contact. The structure design of the utility model ensures that the liquid suction needle can keep static when contacting with the reaction tank, thus solving the problem that the reaction tank is damaged by the firing pin of the liquid suction needle in the prior art; the common cambered surface and the concentric cambered surface on the cam are in smooth transition, so that overlarge impact cannot exist in the contact process of the liquid suction needle and the bottom of the reaction tank, the reaction tank is protected, and the installation stability of the liquid suction needle is ensured.

Description

Liquid transfer mechanism in protein tracing instrument

Technical Field

The utility model relates to the technical field of medical equipment, especially, relate to a move liquid mechanism in protein tracing appearance.

Background

The western blotting technique is a protein separation and detection technique integrating electrophoresis and transfer immunological labeling, and compared with other protein separation techniques, the western blotting technique has the advantages of high sensitivity, convenient operation, high specificity and capability of performing qualitative and semi-quantitative analysis, and is successfully applied to multiple fields of protein property identification and the like at present. Currently, western blot related testing procedures are usually performed on automated western blot instruments.

The automatic protein tracing instrument can detect samples by adding according to experimental requirements, complete processes such as automatic liquid preparation, liquid suction, incubation, washing and substrate adding are completed by using a set operation program, safety guarantee is provided for an operator, errors of manual operation are reduced, reliability is higher, operation is more standard, and time and labor are saved.

The utility model discloses it lies in automatic protein trace appearance and is inhaling the waste liquid in-process to solve technical problem, and the imbibition needle can not effectively laminate the imbibition with the reaction tank to cause the problem of experimental result difference, provide a protein trace appearance to this problem and move liquid mechanism, the device can be in the effectual messenger of certain limit move liquid mechanism and inhale clean waste liquid, very big reduction because the imbibition problem and the unsafe problem of detection that brings.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a move liquid mechanism in protein tracing appearance to solve the problem that provides among the above-mentioned background art.

In order to realize the above-mentioned purpose the utility model provides a technical scheme that adopts is a move liquid mechanism in protein tracing appearance, including imbibition needle, imbibition arm, cam, wherein, the imbibition needle is fixed in on the imbibition arm, the cam with the imbibition arm contact, be equipped with first rotation fulcrum on the imbibition arm, cam motion can drive the imbibition needle winds first rotation fulcrum rotates, the imbibition needle can be used for absorbing the waste liquid in the reaction tank.

Further, the liquid suction device further comprises a supporting element, wherein the supporting element is telescopic or elastic, and the supporting element can enable the liquid suction arm to be always kept in contact with the cam.

Further, the support element is a spring, which is always in tension or compression.

Further, the cam the imbibition arm is located on the mounting panel, the imbibition arm pass through first rotatory fulcrum connect in the mounting panel, still install first motor on the mounting panel, the output shaft of first motor with the cam is connected, first motor can drive the cam is rotatory, the output shaft of first motor is the rotation center of cam.

Further, the cam is provided with a flange surface, a section of concentric arc surface is arranged on the flange surface, and the center point of the concentric arc surface is coincided with the rotation center of the cam.

Further, a point on the flange surface located on the concentric arc surface has the shortest distance from the rotation center of the cam, and when the concentric arc surface is in contact with the liquid suction arm, the liquid suction needle is located at the lowest position and the liquid suction needle just contacts with the bottom of the reaction tank.

Further, the cam is also provided with a common cambered surface, the distance between two adjacent points on the common cambered surface and the rotating central point of the cam is unequal, and when the common cambered surface is contacted with the liquid suction arm, the liquid suction needle can move along with the rotation of the cam driven by the first motor.

Further, the connection part of the common cambered surface and the concentric cambered surface is in smooth transition.

Furthermore, the reaction tanks are arranged on a detection plate, a plurality of reaction tanks are arranged on the detection plate, and the reaction tanks are arranged side by side;

the liquid transfer mechanism further comprises a moving device, and the moving device can drive the liquid suction needle to move, so that the liquid suction needle can suck waste liquid towards the designated reaction tank.

Furthermore, the moving device comprises a synchronous belt mechanism, the synchronous belt mechanism is driven by a second motor, the cam and the liquid suction arm are arranged on an installation plate, the installation plate is connected to a first frame body, the moving device further comprises a second frame body, the second frame body is connected with the first frame body in a sliding fit manner, and the first frame body is connected to a conveying belt of the synchronous belt mechanism through a connecting device;

the mobile device further comprises a positioning sensor, the positioning sensor is mounted on the second frame body and used for monitoring the real-time position of the first frame body and indirectly reflecting the real-time position of the liquid suction needle;

the second frame body is provided with a bulge, and the bulge can limit the first frame body;

when the liquid suction needle extracts waste liquid in the reaction tank, the reaction tank is in a horizontal position or inclines to the position of the liquid suction needle.

To sum up, the utility model has the advantages that:

(1) the utility model discloses a structural design lets the imbibition needle can keep static when contacting with the reaction tank, has solved the imbibition needle firing pin among the prior art, destroys the problem of reaction tank.

(2) The utility model provides a cam go up ordinary cambered surface and concentric cambered surface smooth transition for there can not be too big impact in the bottom contact process of imbibition needle and reaction tank, protected the reaction tank and guaranteed the installation stability of imbibition needle.

(3) The utility model does not need to control the rotating speed of the first motor frequently through a control circuit, thus eliminating the potential risk of the liquid sucking needle and the bottom of the reaction tank generating the firing pin; additionally, utilize the utility model discloses a device, its control circuit is not harsh to first motor rotation angle's control, has solved the problem of the unable clean waste liquid that leads to because of the error among the prior art.

(4) The utility model discloses be equipped with waste liquid overflow alarm device to when the waste liquid in the waste liquid bottle reachs certain capacity, provide the overflow and report to the police.

(5) The utility model discloses two pipeline phase separations of bleeder line, imbibition needle, mutual noninterference, the bleeder line is used for adding new reagent toward the reaction tank in, and the imbibition needle only is used for drawing away the reagent that has detected toward the reaction tank, makes and can not pollute each other between them, consequently when moving liquid structure function and switching, no longer need wash convenient to use.

(6) The utility model discloses all be equipped with the bubble sensor between the route of well waste liquid pump and waste liquid bottle, quantitative syringe and reagent storage bottle, the bubble sensor between quantitative syringe and the reagent storage bottle can be used for monitoring whether to have the bubble in the quantitative syringe, can reflect whether the reagent that quantitative syringe added to the reaction tank can be insufficient; and the bubble sensor between waste liquid pump and the waste liquid bottle can be used for monitoring whether the extraction of waste liquid finishes, and the setting of sensor and control circuit connection can feed back the state of current pipeline, improves the reliability of moving liquid mechanism.

(7) When the liquid suction needle in the liquid transfer mechanism sucks the waste liquid in the reaction tank, the control circuit can control the reaction tank to be in a horizontal position or control the reaction tank to slightly incline towards the position of the liquid suction needle, and the slightly inclined state can enable the waste liquid to flow under the action of gravity, so that the liquid suction needle is favorable for completely discharging the waste liquid in the reaction tank.

Drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings required for the embodiments or the prior art descriptions will be briefly described below, and it is obvious that the drawings in the following description are only one embodiment of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without any creative work.

Fig. 1 is a schematic structural diagram of a pipetting mechanism in a protein track plotter of the utility model in one direction;

fig. 2 is a schematic structural diagram of a liquid-transferring mechanism in the protein track plotter in another direction;

FIG. 3 is a graph of output speed imparted to a first motor in a motion simulation;

FIG. 4 is a graph of the rotational speed achieved for a first pivot point in a motion simulation;

FIG. 5 is a schematic view of a concentric arc on a cam in motion simulation with a suction arm in contact;

fig. 6 is a schematic view of a liquid path structure inside the automatic protein tracing device in an embodiment of the present invention;

FIG. 7 is a schematic view showing the structure of a reaction well on a detection plate;

FIG. 8 is an enlarged schematic view of the structure of the reaction tank of FIG. 7.

Detailed Description

In order to make the technical problems, technical solutions and advantages of the present invention more apparent, the embodiments of the present invention are described in detail with reference to the accompanying drawings, and it should be noted that the embodiments are only detailed descriptions of the present invention, and should not be considered as limitations of the present invention.

In order to make the technical personnel better understand the technical scheme of the utility model, the working mode to protein tracing appearance is briefly summarized earlier: the western blot related test is usually carried out on an automatic western blot instrument, a detection plate 52 is arranged in the automatic western blot instrument, a reaction tank 53 for placing a membrane 54 (the membrane is usually fixed on a membrane strip) and adding a reagent is arranged on the detection plate 52, the membrane needs to be submerged by the reagent, and the membrane and the antigen are fully subjected to fusion reaction by reciprocating swing of a swing mechanism of the instrument, so that an accurate test result is obtained.

After the detection finishes, move liquid mechanism in the protein tracing appearance need with the reagent in the reaction tank 53 is taken out, moves liquid mechanism and includes pipette needle (or pipe), in order to let move liquid mechanism as complete as possible with the reagent clean in the reaction tank, pipette needle in moving liquid mechanism needs the bottom contact with the reaction tank, and the technical problem among the prior art lies in: the accurate control in position of imbibition needle is very difficult, leads to automatic albumen trace appearance at the waste liquid in-process of inhaling, and the imbibition needle can not effectively laminate the imbibition with the reaction tank bottom, leads to the reaction tank in the liquid absorb incompletely, probably causes the difference of experimental result, very big reduction because the imbibition problem and the inaccurate problem of detection that brings.

The utility model discloses a move liquid mechanism in protein mark appearance for arrange the waste liquid in the reaction tank 53 completely, including imbibition needle 101, imbibition needle 101 is fixed in on imbibition arm 104, be equipped with first rotation fulcrum 102 on the imbibition arm 104, imbibition needle 101 can wind first rotation fulcrum 102 rotates, move liquid mechanism still includes cam 103, cam 103 with imbibition arm 104 contacts, forms cam mechanism. Specifically, the cam mechanism further includes a supporting member 105, the supporting member 105 may be retractable or elastic, and functions to keep an end surface of the liquid suction arm 104 in contact with a flange surface of the cam 103 at all times, when the cam 103 starts to rotate, the liquid suction arm 104 can perform a swinging motion around the first rotation fulcrum 102 according to the flange surface of the cam 103, and the movement speed, the movement direction, or the dead time of the liquid suction needle 101 can be controlled by changing the shape of the flange surface of the cam 103.

Preferably, in this embodiment, the supporting member 105 is a spring, one end of which is connected to the first frame 106 and the other end of which is connected to the liquid suction arm 104, so that one end surface of the liquid suction arm 104 is always in contact with the flange surface of the cam 103, thereby forming a cam mechanism. In this embodiment, the liquid suction needle 101 and the cam 103 are connected to both sides of the liquid suction arm 104 with the first rotation fulcrum 102 on the liquid suction needle 101 as a boundary center, and referring to fig. 2, the liquid suction needle 101 is fixed to the left end of the liquid suction arm 104, and the right end of the liquid suction arm 104 is in contact with the cam 103, and in this state, when a spring is connected to the left end of the liquid suction arm 104, the spring is always in a stretched state as long as the liquid suction arm 104 is within a working swing range in order to bring the liquid suction arm 104 into contact with the cam 103; in some other embodiments, when a spring is attached to the right end of the suction arm 104, the spring is always in compression as long as the suction arm 104 is within the operational swing range. Furthermore, in some other embodiments, the pipette needle 101 and the cam 103 are connected to the same side of the pipette arm 104, for example, from left to right: in this state, the liquid suction needle 101, the cam 103, and the first rotation fulcrum 102 are also connected to the liquid suction arm 104 at a position to the left of the first rotation fulcrum 102, and the liquid suction arm 104 is in a stretched state in a swing range of operation.

Preferably, in this embodiment, the cam 103 and the suction arm 104 are both disposed on a mounting plate 107, the suction arm 104 is attached to the mounting plate 107 by the first pivot 102 thereon, the mounting plate 107 is further provided with a first motor 108, an output shaft of the first motor 108 is connected with the cam 103 and can drive the cam 103 to rotate, the mounting plate 107 is connected with the first frame 106, the spring is always in a stretching state, the pulling force generated by the first motor 108 causes the suction arm 104 to engage the cam 103, so that during the driving of the cam 103 by the first motor 108, ensures that the liquid suction needle 101 does not contact with the reaction tank 53 with too large impact, the corresponding cam 103 can be designed according to the size parameters of the reaction tank 53, so as to ensure that the liquid suction port of the liquid suction needle 101 can be located at the bottom of the reaction tank 53 when waste liquid is extracted.

Preferably, the cam 103 has a segment of concentric arc 109, and the arc center point of the concentric arc 109 coincides with the rotation center point of the cam 103 (the output shaft of the first motor 108), that is, as the first motor 108 drives the portion of the concentric arc 109 on the cam 103 to come into contact with the suction arm 104, because the distance between each point on the concentric arc 109 and the rotation center of the cam 103 is the same, the suction arm 104 will not move due to the movement of the cam 103 in the next short period of time, that is, the suction needle 101 on the suction arm 104 will remain stationary in the short period of time. Further, the point of the cam 103 on the concentric arc 109 has the shortest distance from the rotation center of the cam 103, and when the concentric arc 109 on the cam 103 contacts the liquid suction arm 104, the liquid suction needle 101 is located at the lowest position and just contacts the bottom of the reaction tank 53, as shown in fig. 5, because the liquid suction needle 101 is kept stationary at this position, i.e. at zero speed, the liquid suction needle 101 and the bottom of the reaction tank 53 can be prevented from generating a striker, while the prior art liquid transfer mechanism is generally difficult to complete the buffer control of the liquid suction needle, and there is a risk of damaging the reaction tank or the striker. In this example, the cam 103 is further provided with a common arc surface 110, generally, distances between two adjacent points on the common arc surface 110 and a rotation center point of the cam 103 are not equal, when the common arc surface 110 contacts the liquid suction arm 104, the liquid suction needle 101 moves along with the rotation of the cam 103 driven by the first motor 108, and a connection part between the common arc surface 110 and the concentric arc surface 109 is in smooth transition, so that excessive impact does not exist in a contact process between the liquid suction needle 101 and the bottom of the reaction tank 53, the reaction tank 53 (generally made of a plastic material) is protected, and the installation stability of the liquid suction needle 101 is ensured.

Preferably, the first motor 108 is connected to a control circuit, and the control circuit mainly controls start and stop of the first motor 108. The motor in traditional western blot appearance also is connected with control circuit, and the motor passes through the imbibition arm and directly is connected with the imbibition needle, and control circuit in traditional western blot appearance need control a plurality of parameters: the rotation speed, the start and stop and the rotation angle, and the small change of any one of the parameters can greatly affect the liquid suction needle 101: for example, when the liquid suction needle 101 moves to the lowest position, if the control circuit does not control the rotation speed of the first motor 108 to be reduced to zero or the control circuit does not timely turn off the first motor 108 to reduce the rotation speed to zero, the first motor 108 is very easy to drive the liquid suction needle 101 and the bottom of the reaction tank 53 to generate a striker due to the existence of the rotation speed; for another example, if the control circuit controls the first motor 108 to generate a rotation angle deviation, assuming that the first motor 108 controls the pipette needle 101 to rotate twenty degrees, the pipette needle 101 just contacts with the bottom of the reaction tank 53, and if the control circuit fails at this time, causing the first motor 108 to rotate twenty-one degrees, the pipette needle 101 no longer contacts with the bottom of the reaction tank 53, which may cause the pipette needle 101 to be unable to completely discharge the reagent in the reaction tank 53. In the present invention, as long as the concentric arc surface 109 on the cam 103 contacts with the liquid suction arm 104, no matter what the rotation speed of the first motor 108 is, the liquid suction needle 101 on the liquid suction arm 104 can be kept in a static state, i.e. the speed is zero, so that it is no longer necessary to perform frequent rotation speed control on the first motor 108 through a control circuit, and the potential risk of the liquid suction needle 101 and the bottom of the reaction tank 53 causing a striker is eliminated. In addition, utilize the technical scheme of the utility model, control circuit is not so harsh to first motor 108 rotation angle's control, because when concentric cambered surface 109 on the cam 103 with imbibition arm 104 contacts, imbibition needle 101 all be in with the bottom contact of reaction tank 53 and keep quiescent condition, even control circuit breaks down, first motor 108 has rotated once more, has rotated once twice … … for a short time, twice … … as long as concentric cambered surface 109 with imbibition arm 104 still keeps the contact state, just can not influence imbibition needle 101 will the reagent in reaction tank 53 is cleared, has solved among the prior art because the error can't the problem of clearing reagent.

In order to better understand the technical solution of the present invention and to embody the technical effects that can be achieved by the present invention, referring to fig. 3 to fig. 5, a simple motion simulation is performed on the cam 103 and the liquid suction arm 104 in principle, an angular velocity of 15r/min (as shown in table 201 in fig. 3) is given to the first motor 108, that is, the angular velocity of the cam 103 is also 15r/min, an angular velocity curve of the first rotation fulcrum 102 on the liquid suction arm 104 (as shown in table 202 in fig. 4) is obtained, and according to a formula v ═ w × r, the angular velocity curve of the first rotation fulcrum 102 can indirectly reflect the velocity change of the liquid suction needle 101 on the liquid suction arm 104, and the velocity change is proportional to the angular velocity change of the first rotation fulcrum 102. Fig. 5 is a state diagram of the pipette arm 104 when the angular velocity of the first rotation fulcrum 102 is zero, and it can be seen that the left end of the pipette arm 104 is in a state of being swung down to the lowest position (the pipette needle 101 is located on the left end of the pipette arm 104), and at this time, the pipette needle 101 is just in a state of being in contact with the bottom of the reaction well 53. As is clear from fig. 4, the liquid suction needle 101 is in contact with the bottom of the reaction tank 53 for a period of time and is kept in a stationary state, and the movement speed of the liquid suction needle 101 is gradually reduced and gradually increased during the process that the liquid suction needle 101 approaches or leaves the bottom of the reaction tank 53, which effectively prevents the liquid suction needle 101 from striking the bottom of the reaction tank 53. The utility model provides a move liquid mechanism in western blot appearance can accomplish the imbibition operation through design and circuit control cooperation on the machinery to reduce the risk of firing pin, the adaptation environmental change causes the imbibition unclean and the deviation of the testing result that leads to.

In general, the liquid-transferring mechanism needs to provide a liquid-absorbing power to absorb the waste liquid in the reaction tank 53, and the most common liquid-absorbing power is a negative pressure, referring to fig. 6, the liquid-transferring mechanism further includes a liquid-absorbing pump 111 connected to the liquid-absorbing needle 101, the waste liquid pump 111 provides power to absorb the waste liquid, and the other end of the waste liquid pump 111 is connected to a waste liquid bottle 112. The negative pressure is established by the waste liquid pump 111, the liquid suction needle 101 starts to suck waste liquid, the liquid suction needle 101 sucks waste liquid from the reaction tank 53 and discharges the waste liquid into the waste liquid bottle 112 through the waste liquid pump 111, and the waste liquid pump 111 is controlled by a control circuit so as to complete liquid suction in a correct reaction tank.

Preferably, the waste liquid pump is connected to a control circuit, a waste liquid overflow alarm device 113 is disposed at the upper end of the waste liquid bottle 112 so as to provide an overflow alarm when the waste liquid in the waste liquid bottle 112 reaches a certain volume, and the waste liquid overflow device 113 is connected to the control circuit so as to provide an alarm and stop the waste liquid suction operation when the waste liquid in the waste liquid bottle 112 reaches a set volume.

In some embodiments, the pipette needle 101 can perform both operations of withdrawing waste liquid and adding new reagent, and since both the operations of withdrawing waste liquid and adding new reagent are performed by the pipette needle 101, in order to avoid the waste liquid remaining in the pipette needle 101 from contaminating the newly added reagent, the pipette needle 101 needs to be cleaned at the switching interval between the steps of withdrawing waste liquid and adding new reagent, and the cleaning operation can be manual or automatic.

In this embodiment, the liquid transferring structure further includes a liquid discharging tube 114, the liquid discharging tube 114 is used for adding a new reagent into the reaction tank 53, and the liquid sucking needle 101 is only used for sucking away the detected reagent from the reaction tank 53, so that the new reagent is added, and the waste liquid is separated through two pipelines, which will not pollute each other, and therefore, when the function of the liquid transferring structure is switched, cleaning is not needed any more. Meanwhile, the liquid suction needle 101 needs to be in contact with the bottom of the reaction tank 53 when the waste liquid is drawn away, so that the waste liquid in the reaction tank 53 can be discharged, and when the liquid discharge pipe 114 adds a reagent into the reaction tank 53, the liquid discharge pipe 114 does not need to be in contact with the reaction tank 53, so that the liquid discharge pipe 114 is prevented from being contaminated by the waste liquid remaining in the reaction tank 53. Further, the liquid discharging tube 114 is connected to a quantitative injector 115 or a quantitative pump, so that a quantitative reagent can be added into the reaction tank 53 through the liquid discharging tube 114, and a quantitative test can be realized, the quantitative injector 115 is further connected to a reagent storage bottle 116, the reagent storage bottle 116 is used for storing a reagent, and the quantitative injector 115 can extract the reagent from the reagent storage bottle 116. Preferably, the quantitative injector 115 is connected to an electromagnetic valve 117, and the electromagnetic valve 117 can switch the pipeline connected to the quantitative injector 115, thereby switching the connection between the quantitative injector 115 and the reagent storage bottle 116 and the connection between the quantitative injector 115 and the liquid discharge tube 114.

Preferably, the liquid discharging pipes 114 are provided with a plurality of liquid discharging pipes 114, as shown in fig. 2 and fig. 6, these different liquid discharging pipes 114 can be used for adding different reagents into the reaction tank 53, and different reagents are added through the different liquid discharging pipes 114, so that mutual contamination between different reagents can be avoided. Preferably, the liquid discharge tubes 114 are fixed on a support 118, and the liquid discharge tubes 114 are arranged in a straight line to adapt to the shape (generally, a long strip shape) of the reaction tank 53, so as to facilitate the addition of the reagent. Preferably, the support 118 is fixed to the first frame 106 so that the liquid discharge tube 114 can move together with the liquid suction needle 101.

Preferably, sensors are disposed between the waste liquid pump 111 and the waste liquid bottle 112, and between the quantitative syringe 115 and the reagent storage bottle 116, and the sensors may be bubble sensors or some other sensors commonly used in the art. In this embodiment, the bubble sensor between the quantitative injector 115 and the reagent storage bottle 116 can be used to monitor whether bubbles exist in the quantitative injector 115, which can reflect whether the reagent added into the reaction tank 53 by the quantitative injector 115 is insufficient. And a bubble sensor between the waste liquid pump 111 and the waste liquid bottle 112 can be used for monitoring whether the waste liquid is completely extracted. The sensors are connected with the control circuit, and can feed back the current state of the waste liquid pipeline, so that the reliability of the liquid transfer mechanism is improved.

Considering that a plurality of reactions are generally required to be performed in the automatic western blotting apparatus, most of the automatic western blotting apparatuses currently on the market have a plurality of reaction tanks 53, and the reaction tanks 53 are arranged side by side, as shown in fig. 7 and 8, membranes are fixed on membrane strips 54, and the membrane strips 54 are placed at the bottoms of the reaction tanks 53. In order to let move liquid mechanism and can go up the measuring on appointed reaction tank 53 to after the measuring, can inhale the waste liquid operation to appointed reaction tank 53, the utility model discloses in, move liquid mechanism in a protein trace appearance still includes mobile device, and this mobile device can drive move liquid mechanism in the liquid discharge pipe 114 inhale liquid needle 101 moves, lets liquid discharge pipe 114 realize toward appointing add reagent in the reaction tank 53, inhale liquid needle 101 and realize toward appointing take away the waste liquid in the reaction tank 53.

Preferably, in this embodiment, the moving device includes a synchronous belt mechanism 119, the synchronous belt mechanism 119 is driven by a second motor 120, the moving device further includes a second frame body 121, the second frame body 121 is connected with the first frame body 106 in a sliding fit manner, specifically, the second frame body 121 is connected with the first frame body 106 in a sliding fit manner through a slide block slide rail 125. In some other embodiments, the first frame 106 can also move linearly on the second frame 121 by other means, such as a lead screw slider, a linear motor, a hydraulic push rod, etc. The first frame 106 is connected to the conveyor belt of the timing belt mechanism 119 through a connecting device 122, the connecting device 122 may be a clip, and the fixing of the first frame 106 on the timing belt mechanism 119 is completed by clamping the conveyor belt of the timing belt mechanism 119. Work as the drive of second motor 120 during synchronous belt mechanism 119 moves, synchronous belt mechanism 119 passes through connecting device 122 drives first support body 106 is in slide on the second support body 121, on first support body 106 the tapping pipe 114 the imbibition needle 101 can be along with first support body 106 moves together, through control the accurate motion of first support body 106 can be realized to second motor 120 pivoted number of turns and/or angle, lets the tapping pipe 114 the imbibition needle 101 aims at appointed reaction tank 53, realizes the reagent of appointed reaction tank 53 and adds and the waste liquid operation of taking away.

Preferably, the moving device further includes a positioning sensor, the positioning sensor is installed on the second frame body 121, the positioning sensor may be provided with a plurality of sensors for monitoring the real-time position of the first frame body 106 on the sliding block sliding rail 125, and the real-time position of the first frame body 106 can reflect the real-time position of the liquid discharge tube 114 and the liquid suction needle 101.

Preferably, two protrusions 126 protruding upwards are respectively arranged at two ends of the slider sliding rail 125 on the second frame body 121, and the protrusions 126 can limit the slider sliding rail 125, so as to prevent the slider from falling off due to excessive movement of the slider sliding rail 125.

Reaction tank in the automatic protein trace plotter can carry out the rocking of certain angle for reagent can be abundant with the membrane strip reaction, control circuit can control reaction tank 53 sways the assigned position. When the liquid discharging tube 114 in the liquid transferring mechanism in the automatic protein tracing instrument needs to add reagent, the control circuit controls the reaction tank 53 to be in a horizontal position, the liquid discharging tube 114 starts to add reagent into the reaction tank 53, and the reaction tank 53 in the horizontal state is not easy to overflow the reagent; when in the automatic egg white mark appearance inhale liquid needle 101 among the liquid-transfering mechanism need will when the waste liquid in the reaction tank 53 is taken out, control circuit control reaction tank 53 to horizontal position or control circuit control reaction tank 53 to inhale liquid needle 101 position slope slightly, it needs to be noted that can not lead to under the slightly inclined state waste liquid in the reaction tank 53 spills, and the slightly inclined state can make the waste liquid flow under the effect of gravity, helps inhale liquid needle 101 will the waste liquid in the reaction tank 53 is arranged completely.

The above description is only the specific embodiments of the present invention, but the protection scope of the present invention is not limited thereto, and any changes or substitutions that are not thought of through creative work should be covered within the protection scope of the present invention, and therefore, the protection scope of the present invention should be subject to the protection scope defined by the claims.

Claims (10)

1. The utility model provides a move liquid mechanism in protein trace appearance, a serial communication port, including imbibition needle, imbibition arm, cam, wherein, the imbibition needle is fixed in on the imbibition arm, the cam with the imbibition arm contacts, be equipped with first rotation fulcrum on the imbibition arm, cam motion can drive the imbibition needle winds first rotation fulcrum rotates, the imbibition needle can be used for absorbing the waste liquid in the reaction tank.

2. The pipetting mechanism in a protein blotter of claim 1, further comprising a support member that is telescopic or resilient, the support member allowing the pipetting arm to remain in contact with the cam at all times.

3. The pipetting mechanism in a protein blotting instrument of claim 2 wherein the support element is a spring, the spring being either in tension or in compression at all times.

4. A liquid-transfering mechanism in protein track appearance according to claim 1, characterized in that, the cam the imbibition arm is located on the mounting panel, the imbibition arm pass through first rotatory fulcrum connect in the mounting panel, still install first motor on the mounting panel, the output shaft of first motor with the cam is connected, first motor can drive the cam is rotatory, the output shaft of first motor is the centre of rotation of cam.

5. The pipetting mechanism of claim 4, wherein the cam has a flange surface with a segment of concentric arc surface, and the center point of the concentric arc surface coincides with the center of rotation of the cam.

6. A pipetting mechanism in a protein blotting instrument as recited in claim 5 wherein the point on the face of the flange on the concentric arc is at the shortest distance from the center of rotation of the cam, and when the concentric arc is in contact with the pipetting arm, the pipetting needle is at the lowest position and the pipetting needle is just in contact with the bottom of the reaction well.

7. The pipetting mechanism in the protein track plotter according to claim 5, wherein the cam is further provided with a common cambered surface, the distance between two adjacent points on the common cambered surface and the rotation central point of the cam is unequal, and when the common cambered surface is in contact with the pipetting arm, the pipetting needle moves along with the rotation of the cam driven by the first motor.

8. The pipetting mechanism in a protein blotting instrument of claim 7 wherein the generally arcuate surface smoothly transitions with the connecting portion of the concentric arcuate surface.

9. The pipetting mechanism in the protein blotting instrument as recited in claim 1, wherein said reaction tank is arranged on a detection plate, said detection plate is provided with a plurality of reaction tanks, and the reaction tanks are arranged side by side;

the liquid transfer mechanism further comprises a moving device, and the moving device can drive the liquid suction needle to move, so that the liquid suction needle can suck waste liquid towards the designated reaction tank.

10. The pipetting mechanism in the protein track plotter according to claim 9, wherein the moving device comprises a synchronous belt mechanism, the synchronous belt mechanism is driven by a second motor, the cam and the liquid suction arm are arranged on a mounting plate, the mounting plate is connected to a first frame body, the moving device further comprises a second frame body, the second frame body is connected with the first frame body in a sliding fit manner, and the first frame body is connected to a conveying belt of the synchronous belt mechanism through a connecting device;

the mobile device further comprises a positioning sensor, the positioning sensor is mounted on the second frame body and used for monitoring the real-time position of the first frame body and indirectly reflecting the real-time position of the liquid suction needle;

the second frame body is provided with a bulge, and the bulge can limit the first frame body;

when the liquid suction needle extracts waste liquid in the reaction tank, the reaction tank is in a horizontal position or inclines to the position of the liquid suction needle.

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