Utility model content
The purpose of this utility model is to provide a kind of immunofluorescence quantitative analysis instrument, volume is small and exquisite, easy and simple to handle, safeguard easily, stability and sensitivity behaviour high.This instrument can be used for other detection of centralab, outpatient service/emergency treatment laboratory, CCU, ICU, clinical laboratory, clinical department and bed of medical institutions.
A kind of immunofluorescence quantitative analysis instrument, comprise housing, also comprise stepper motor, stepper motor and the first belt pulley are coupling, in housing, be provided with the second belt pulley, between the first belt pulley and the second belt pulley, by belt, be connected, on belt, be fixed with specimen holder belt contiguous block, specimen holder belt contiguous block is fixedly connected with slide block, slide block can be along being arranged on the slide rail to-and-fro movement in housing, slide block is connected with specimen holder, specimen holder bottom offers detects hole, also comprise by photovoltaic element adjustment rack and be fixed on housing and be positioned at the photovoltaic element of specimen holder below, photovoltaic element sidepiece is provided with bar code detectors or Quick Response Code detecting device.
One end of specimen holder to-and-fro movement stroke as above is provided with light groove, and specimen holder is provided with banking stop corresponding to the position of light groove.
Photovoltaic element as above comprises shell, also comprise the 3rd lens that setting is placed in the enclosure from top to bottom successively, the second optical filter, the 4th lens and for detection of the fluoroscopic examination module of the 4th lens transmission fluorescence, also comprise and set gradually the second lens in the enclosure, the first optical filter, first lens and for generation of light source and project the LED light source container of first lens, the 3rd lens, the second optical filter, the optical axis coincidence of the 4th lens forms primary optic axis, the second lens, the first optical filter, the optical axis coincidence of first lens forms the second optical axis, primary optic axis becomes predetermined angle with the second optical axis, the intersection point of primary optic axis and the second optical axis is the focus of the 3rd lens and the second lens.
Predetermined angle as above is 45 degree, and the intersection point of primary optic axis and the second optical axis is positioned at 3-8mm place, shell top.
In LED light source container as above, be also provided with the photoelectricity intensity detection module for detection of light intensity.
Testing process is: the test card to be checked that test strip is housed is filled in specimen holder 5, bar code detectors 4/ Quick Response Code detecting device can be surveyed test card and whether be put into position, if be put into position, so under the driving of stepper motor 12, belt 8 can drive specimen holder 5 toward the 9 direction motions of light groove, now, bar code detectors 4/ Quick Response Code detecting device can read the barcode data of air brushing on test card.When the banking stop 17 of specimen holder 5 arrives in the groove of light grooves 9, light groove 9 can provide a spacing detection signal, and specimen holder 5 stops the motion of original direction, is exactly the origin position of specimen holder here.Afterwards, stepper motor 12 rotates backward, specimen holder 5 is also along with counter motion, photovoltaic element 2 is started working, the LED light source that LED light source container 19 sends projects in the test strips on test card through first lens 20, the first optical filter 21, the second lens 22 successively, the fluorescence producing is incident upon fluoroscopic examination module 26 by the 3rd lens 23, the second optical filter 24, the 4th lens 25 successively) on collect, and convert electric signal to, amplify, filtering and analog to digital conversion, realize fluorescence intensity to the conversion of digital signal.Test card is often walked a small step, just gathers fluorescent intensity data and stores, and after whole test strips setting regions been scanned, these fluorescent intensity data just can form a sample sequence curve.Standard working curve by the curve to gathered and storage is in advance analyzed, and can draw final testing sample concentration information.Final analysis result can show, prints or network output.
Slide rail that the utility model has adopted 7, the single accurate miniature line slide rail of first-selected employing.Mostly at present existing similar devices, be to adopt two circular line slideways.The utility model only needs single accurate line slide rail, and slide block on slide rail, move will be more stable than two circular guideways, speed is faster, long-term dependability is high.
Photovoltaic element 2 of the present utility model, comprises opticator and electronics two parts, and they are encapsulated in a box the inside.Opticator is comprised of source emissioning light road and fluorescence detection optical path two parts, and electronics part is comprised of light emitting control and two parts of fluoroscopic examination circuit.Light source adopts small packaged high-power LED lamp pearl, adopts interference filter to filter to light source, and the first-selected bandpass filter of interference filter, also can select short-pass cutoff filter.Fluoroscopic examination adopts spike interference filter to carry out fluorescence, and the first-selected silicon photo diode of opto-electronic conversion also can be selected the opto-electronic conversion chip of built-in amplifier.Light emitting control adopts high precise current source to power to LED, can guarantee the stable of light source luminescent intensity, and meanwhile, the intensity of light source adopts digital-to-analog conversion to control, and by inputting different digital values, can control the luminous intensity of LED.Fluoroscopic examination circuit has comprised the parts such as opto-electronic conversion, signal amplification filtering, voltage bias, analog to digital conversion, wherein, signal amplifies the program control amplification of employing, and analog to digital conversion adopts the A/D conversion chip of 24, can guarantee enough conversion accuracies and large dynamic detection range.
The specimen holder 5 that the utility model adopts, has adopted detachable design, can quick-replaceable specimen holder, be adapted to the detection application of variety classes sample card.
The bar code detectors 4 that the utility model adopts, adopts reflective probe, and first-selection utilizes infrared light supply LED to throw light on, and can detect the bar code of air brushing on test card.The illumination of employing infrared LED, can prevent the interference of natural light.Bar code detectors 4, can adopt single-point type detecting device, also can adopt Quick Response Code detecting device.While adopting single-point type detecting device, can Test paper card on the simple and easy bar code of air brushing.While adopting Quick Response Code detecting device, can Test paper card on the Quick Response Code of air brushing.If adopt Quick Response Code detecting device, we can, by the air brushing of typical curve data on test card, save the trouble of the external IC-card storage standards curves of conventional fluorescent detecting device needs.
The Electronic Control that the utility model adopts and display system, adopt ARM (the Advanced RISC Machines) processor of 32 as main control chip, the function such as its control, the collection of fluorescence digital signal, the control of motor, data analysis that can realize luminous intensity is printed with demonstration, result, man-machine interface operation, data are stored, communicated by letter.
Adopt after technique scheme, contrast prior art, the beneficial effects of the utility model are:
1, adopt single precise linear guide, volume is little, and makes specimen holder motion more steadily fast.
2, optics and portions of electronics are encapsulated in a photovoltaic element, system is debug simpler, and vibration resistance is stronger.
3, adopt detachable specimen holder, can quick-replaceable specimen holder, be adapted to the detection application of variety classes sample card.
4, the partial circuit of fluoroscopic examination is placed in photovoltaic element, has adopted program control Enlargement Design and 24 A/D conversion chips, the precision that system is detected is higher, and dynamic detection range is wider.
5, adopt Quick Response Code, realized immediately reading of the characteristics such as typical curve, saved the trouble of external IC-card preservative feature data.
Embodiment
Below in conjunction with accompanying drawing, embodiment of the present utility model is done to further detailed description.
As shown in Fig. 1-6, a kind of immunofluorescence quantitative analysis instrument, comprise housing 1, also comprise stepper motor 12, stepper motor 12 and the first belt pulley 10 are coupling, in housing 1, be provided with the second belt pulley 13, between the first belt pulley 10 and the second belt pulley 13, by belt 8, be connected, on belt 8, be fixed with specimen holder belt contiguous block 16, specimen holder belt contiguous block 16 is fixedly connected with slide block 6, slide block 6 can be along being arranged on slide rail 7 to-and-fro movements in housing 1, slide block 6 is connected with specimen holder 5, specimen holder 5 bottoms offer detects hole, also comprise by photovoltaic element adjustment rack 3 and be fixed on housing 1 and be positioned at the photovoltaic element 2 of specimen holder 5 belows, photovoltaic element 2 sidepieces are provided with bar code detectors 4 or Quick Response Code detecting device.
One end of specimen holder 5 to-and-fro movement strokes is provided with light groove 9, and specimen holder 5 is provided with banking stop 17 corresponding to the position of light groove 9.
Photovoltaic element 2 comprises shell 18, also comprise and be arranged on the 3rd lens 23 of placing successively from top to bottom in shell 18, the second optical filter 24, the 4th lens 25 and for detection of the fluoroscopic examination module 26 of the 4th lens 25 transmission fluorescence, also comprise and be successively set on the second lens 22 in shell 18, the first optical filter 21, first lens 20 and for generation of light source and project the LED light source container 19 of first lens 20, the 3rd lens 23, the second optical filter 24, the optical axis coincidence of the 4th lens 25 forms primary optic axis, the second lens 22, the first optical filter 21, the optical axis coincidence of first lens 20 forms the second optical axis, primary optic axis becomes predetermined angle with the second optical axis, the intersection point of primary optic axis and the second optical axis is the focus of the 3rd lens 23 and the second lens 22.
Predetermined angle is 45 degree, and the intersection point of primary optic axis and the second optical axis is positioned at shell 18 3-8mm places, top.
In LED light source container 19, be also provided with the photoelectricity intensity detection module for detection of light intensity.
Fig. 1 is integral installation figure of the present utility model.Mainly comprise: housing 1, photovoltaic element 2, photovoltaic element adjustment rack 3, bar code detectors 4, specimen holder 5, slide block 6, slide rail 7, belt 8, light groove 9, the first belt pulley 10, the second belt pulley 13, motor rack 11, stepper motor 12, ball bearing 14, ball-bearing housing 15, specimen holder belt contiguous block 16, and Electronic Control and display system etc.
Fig. 2 is the slide rail that adopts of the utility model and the structural representation of slide block.Slide rail 7 adopts single precise linear guide, compares with the normal circular guideway adopting of product of the same type, and its thickness is thicker, and width is wider, and therefore, its anti-vibration is very competent, and bearing capacity is also very good.Slide rail 7 has row's threaded mounting hole above, can be subject under extraneous strong Vibration Condition by screw by its firm top that is fixed on instrument housing 1, and it can not produce deformation, therefore, has good reliability.Slide block 6 is arranged on slide rail 7, and the utility model adopts double-row balls expanded letter slide block, and ball matches with the groove of both sides on slide rail 7.Slide block 6 can be free to slide on slide rail 7, owing to having adopted ball slider, therefore, no matter compare with the normal circular guideway mode of motion adopting of like product, in the utility model, be travelling speed, or the smoothness of motion will be higher, and the noise in when motion is less, long-term use without lubricated.In addition, the utility model only needs an one slide rail, and conventional two guide rails of product needed of the same type.
Fig. 3 is the structural representation of the running part that adopts of the utility model.The utility model adopts stepper motor to drive, belt transmissioning mode.Can adopt diameter dimension be the stepper motor 12 of 42mm as drive source, stepper motor 12 is fixed on motor rack 11, motor rack 11 is fixed on the rear plate of housing 1.The first belt pulley 10 is coaxial with stepper motor 12, and the second belt pulley 13 is coaxial with ball bearing 14, and belt 8 is enclosed within on the first belt pulley 10 and the second belt pulley 13, and when stepper motor 12 rotates, belt 8 moves under the rotation of the second belt pulley 13 drives.Belt 8 is connected by screw with contiguous block 16 with the parallel placement of slide rail 7, belt 8, and contiguous block 16 is closely connected by screw with slide block 6 with specimen holder 5 again.When belt 8 motion, it can drive specimen holder 5 to do along the rectilinear motion of slide rail direction together with slide block 6.Therefore, the rotation of stepper motor 12 can be converted to the rectilinear motion of specimen holder 5 here, when direction of motor rotation is reverse, the direction of motion of specimen holder 5 also can be reverse.
Fig. 4 is the structural representation of stop portion of the present utility model.When specimen holder 5 is done rectilinear motion, in order to protect specimen holder can not bump against on housing 1, need to there is a spacing position, when specimen holder moves to this position, must automatically stop, otherwise can damage this equipment.Here, spacing control we adopt photoelectric position limit mode.Light groove 9 is fixed on the rear plate of housing 1, at the afterbody of specimen holder 5, has an outstanding banking stop 17.When specimen holder 5 moves to the extreme position setting, banking stop 17 can insert light groove 9, and block the light path of light groove 9 interior correlations, make the circuit output of light groove 9 produce logic saltus step, this transitional states can be sent to the central processing circuit of this instrument, thereby control step motor no longer sends the motion impulse of original direction, guarantee that specimen holder 5 can not continue to move along original direction at this extreme position.This extreme position zero point that namely we set, when sample scans, to using this as coordinate zero point, (when sample scans, specimen holder 5 is positioned at extreme position, the position of the sample of photovoltaic element 2 correspondences is the reference position of scanning), according to stepper motor umber of pulse, carry out location positioning.
Fig. 5 is optical unit external structure schematic diagram of the present utility model.Opticator mainly contains several parts and forms: photovoltaic element 2, photovoltaic element adjustment rack 3, bar code detectors 4.Photovoltaic element 2 mainly completes the generation of fluorescence excitation light source and the collection of fluorescence signal detects, bar code detectors 4 is mainly for detection of the bar code on the sampling test card of loading onto in specimen holder 5, meanwhile, its another one function is the position whether Test paper card is inserted into regulation.Photovoltaic element adjustment rack 3 is fixed on the base plate of housing 1, between itself and photovoltaic element 2, be screwed, relative position between the two can regulate, by adjusting relative position, make the light path of photovoltaic element 2 face the sample expose portion on test card, and make utilizing emitted light focus just in time focus on test paper surface.
Bar code detectors 4 adopts reflective photoelectric sensor, it includes a light emitting diode and a Photoelectric Detection pipe, when not having thing to be arranged in light path, or the target of black is while being arranged in light path, Photoelectric Detection pipe will detect a fainter background signal, when adularescent object appears in transmitting light path, just has light and reflects, by Photoelectric Detection pipe, received, thereby can survey, whether adularescent target is in light path.At specimen holder 5, when until sample introduction position, bar code detectors 4 installation sites face the terminal position of specimen holder 5.Therefore, when having test card to be inserted into the terminal position of specimen holder, bar code detectors 4 just can detect the light that the surface of test card is reflected back, thereby perception test card has been inserted into position, and then automatically starts whole measuring process.The another one function of bar code detectors 4 is detections of bar code, test card is generally white, above it, be sprayed with the bar code of black, hence one can see that, when test card moves with belt 8, bar code will be inswept from the photoelectric reflection face of bar code detectors 4, thereby cause the variation that detects light intensity, by detecting this light intensity, we just can restore digitized bar code by central processing unit.Here, in order to prevent that test card from having made dirty, cause the situation of Photoelectric Detection failure, we have adopted infrared light supply the light source of bar code detectors 4, and it has good interference free performance.
It should be noted that, the utility model is not limited to the detection of simple one-dimensional bar code.Two-dimensional bar is as a kind of more senior means, and we also can detect.We,, at a catoptron of the 45 ° of placements in the adjacent position of bar code detectors 4, place Quick Response Code detecting device in side, can detect the two-dimensional bar of air brushing on test card.
Photovoltaic element 2 has comprised light source transmitting and has produced, phosphor collection, and several parts such as fluorescence intensity photoelectricity numeralization, are encapsulated in a box the inside.Its major function is: produce the excitation source of satisfactory irradiation sample, and the fluorescence ejecting is efficiently collected, remove to greatest extent the interference of bias light, fluorescence intensity is carried out to opto-electronic conversion, pass to central processing unit after digitizing.As shown in Figure 6, it mainly comprises its inner structure: shell 18, LED light source container 19, first lens 20, the second lens 22, the 3rd lens 23, the 4th lens 25, the first optical filter 21, the second optical filter 24, transmitting illuminant control module 28, fluoroscopic examination module 26.The optical axis of LED light source container 19 and first lens 20, the second lens 22, and the optical axis of the first optical filter 21 is on a line, it is optical axis coincidence, form primary optic axis, the optical axis of fluoroscopic examination module 26 optical detection devices and the 3rd lens 23, the 4th filter 25, the second optical filter 24 is on a line, be optical axis coincidence, form the second optical axis.Primary optic axis becomes 45 degree with the second optical axis, and its intersection point is 3 ~ 8mm above the shell from shell 18, and intersection point is the focus of the second lens 22 and the 3rd lens 23.The first optical filter 21 and the second optical filter 24 are interference filter, all adopt spike interference filter.Wherein, the first optical filter optical density value is OD4, the second optical filter optical density value is not less than OD4. the first optical filter bandwidth and is not less than 20nm (the present embodiment adopts 30nm), the second optical filter bandwidth is no more than 25nm (the present embodiment adopts 15nm), and wherein the first optical filter 21 also can be used the short-pass interference filter that cutoff wavelength is 580nm.
Light path principle is as follows: the LED of LED light source container 19 interior installations sends the light beam of a misconvergence of beams, and LED is positioned in the focus of first lens 20, thereby becomes directional light after first lens 20.Directional light is after the first optical filter 21, above (the note: different detection demands of 580nm in incident light, interchangeable different optical filter, therefore, this wavelength is not what fix) light filtered out, the light of outgoing is short wavelength light or narrow band light (580nm is following), they are after the second lens 22, be focused onto on sample, inspire fluorescence.The fluorescence inspiring becomes directional light after being collected by the 3rd lens 23, and directional light is by after the second optical filter 24, and exciting light originally and parasitic light can be filtered out by optical filter, and only the surplus fluorescence signal inspiring passes through.By the light after optical filter, by the 4th lens 25, collected and focus on the photoelectric commutator in fluoroscopic examination module 26, realize light signal to the conversion of electric signal.
The interior installation of LED light source container 19 green (General Central wavelength is at 525nm) or blue (centre wavelength 485nm) LED light source, LED adopts high-brightness LED lamp pearl as light source, in order to guarantee the stability of light source, adopt high stability constant current source type of drive, can effectively guarantee the constant of light source luminescent intensity like this.For the sample having, radiation source should not be too strong, and therefore, what constant current source adopted is the constant current source of program control adjusting, by inputting different digital values, can control the luminous intensity of LED.The size that is LED drive current can be controlled by program.The whether normal performance that is directly connected to instrument due to light source, in order to strengthen the reliability of instrument, in the groove 27 of the LED light source container 19 shown in Fig. 6, a photoelectricity intensity detection module has been installed, for automatically detecting the only no intensity that is stabilized in setting that LED sends.
The interior installation Photoelectric Detection of fluoroscopic examination module 26 module.The first-selected silicon photo diode of opto-electronic conversion, also can select the opto-electronic conversion chip of built-in amplifier.Fluoroscopic examination circuit has comprised the parts such as opto-electronic conversion, signal amplification filtering, voltage bias, analog to digital conversion.Opto-electronic conversion realizes fluorescence signal to the conversion of electric signal, and signal is now also fainter, and with noise, need to amplify and filtering through signal.Amplification and the general baseline of filtered signal are higher, pass through voltage bias, baseline is lowered, then by analog to digital conversion circuit, realize the digitizing of signal, by microprocessor, carried out follow-up processing, wherein, signal amplifies the program control amplification of employing, and analog to digital conversion adopts the A/D conversion chip of 24, can guarantee enough conversion accuracies and large dynamic detection range.Fluoroscopic examination module 26 is arranged in this metal box, can effectively guarantee that signal is not subject to external interference, thereby greatly improve the precision of system.
For specimen holder 5, adopted detachable specimen holder.Due to different testing goals, the structure of possible test card is different, therefore, same instrument, changes different test cards, therefore possibly, we have adopted a kind of detachable specimen holder structure, need not take instrument apart, fast changeable specimen holder, applies to be adapted to the detection of variety classes sample card.
Electronic Control and display system for system, adopt ARM (the Advanced RISC Machines) processor of 32 as main control chip, its control, the collection of fluorescence digital signal, the control of motor, data analysis that can realize luminous intensity and demonstration, result printing, man-machine interface operation, data storage, instrument self checking, the function such as communicate by letter.Display screen adopts 6 cun of above screens of the LED with touch-screen, adopts the graphical interaction interface that is similar to Android or WinCE.In order to adapt to different application demands, touch-screen is equipped with touch screens and two kinds of configurations of capacitance plate.Capacitance plate is suitable for naked finger operation, more as mobile phone operation.Touch screens is suitable for being with gloves to operate.Test result can show, also can pass through built-in thermal-sensitive printer printing.The test result of different periods can be stored in the permanent storage that plate carries, convenient search and carry out trend and check.
Specific embodiment described herein is only to the explanation for example of the utility model spirit.The utility model person of ordinary skill in the field can make various modifications or supplements or adopt similar mode to substitute described specific embodiment, but can't depart from spirit of the present utility model or surmount the defined scope of appended claims.