CN203602617U - Device for separating mononuclear cells from whole blood - Google Patents
Device for separating mononuclear cells from whole blood Download PDFInfo
- Publication number
- CN203602617U CN203602617U CN201320832225.4U CN201320832225U CN203602617U CN 203602617 U CN203602617 U CN 203602617U CN 201320832225 U CN201320832225 U CN 201320832225U CN 203602617 U CN203602617 U CN 203602617U
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- centrifuge tube
- whole blood
- separation
- permeable membranes
- semi
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- Expired - Fee Related
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- 210000005087 mononuclear cell Anatomy 0.000 title claims abstract description 30
- 210000004369 blood Anatomy 0.000 title claims abstract description 26
- 239000008280 blood Substances 0.000 title claims abstract description 26
- 239000012528 membrane Substances 0.000 claims abstract description 26
- 238000000926 separation method Methods 0.000 claims description 24
- -1 polyethylene terephthalate Polymers 0.000 claims description 3
- 229920000139 polyethylene terephthalate Polymers 0.000 claims description 3
- 239000005020 polyethylene terephthalate Substances 0.000 claims description 3
- 229920000915 polyvinyl chloride Polymers 0.000 claims description 3
- 239000004800 polyvinyl chloride Substances 0.000 claims description 3
- 210000005259 peripheral blood Anatomy 0.000 abstract description 2
- 239000011886 peripheral blood Substances 0.000 abstract description 2
- 210000003819 peripheral blood mononuclear cell Anatomy 0.000 abstract 2
- 238000005119 centrifugation Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 description 6
- 238000010586 diagram Methods 0.000 description 4
- 210000004698 lymphocyte Anatomy 0.000 description 4
- 239000000243 solution Substances 0.000 description 3
- 229920001917 Ficoll Polymers 0.000 description 2
- 238000001467 acupuncture Methods 0.000 description 2
- 210000000601 blood cell Anatomy 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 238000002659 cell therapy Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 210000004700 fetal blood Anatomy 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
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- Centrifugal Separators (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model discloses a device for separating mononuclear cells from whole blood, comprising a centrifuge tube and an upper cover, wherein the top of the centrifuge tube is open, and the bottom of the centrifuge tube is closed; the upper cover is closely connected to the top of the centrifuge tube; and a semipermeable membrane is arranged inside the centrifuge tube and used for dividing the inside part of the centrifuge tube into two parts. The device disclosed by the utility model can be used for directly obtaining high-purity peripheral blood mononuclear cells (PNMC) by directly pouring peripheral blood into the centrifuge tube for centrifugation, thus the operation time is saved greatly and the quantity of the obtained PBMC is high.
Description
Technical field
The utility model relates to cell culture technology field, relates in particular to a kind of device of separation of whole blood mononuclearcell.
Background technology
Whole blood refers to peripheral blood or Cord blood, mononuclearcell in whole blood is as generating in vitro many cellulous raw materials, in fundamental research and cell therapy field, have demand widely, the method for the mononuclearcell in extraction whole blood at present used is traditional Ficoll method.
Contriver research process in find, there is following defect in the method: length consuming time, whole step need to reach 1.5 hours; Operating process is loaded down with trivial details, and it is especially careful in the time adding sample and extract tunica albuginea layer, to need; Target cell purity is not high.
Utility model content
The utility model provides a kind of device of separation of whole blood mononuclearcell, has greatly shortened disengaging time, simple to operate, purity is higher.
The utility model provides a kind of device of separation of whole blood mononuclearcell, comprises centrifuge tube, upper cover, the open top of described centrifuge tube, closed bottom, and described upper cover is closely connected to the top of described centrifuge tube; A semi-permeable membranes is installed in inside at described centrifuge tube, and described semi-permeable membranes is for separating lymphocyte separation medium and complete blood cell, and the interior separation of described centrifuge tube is become two portions by described semi-permeable membranes.
Uniform several coniform holes on described semi-permeable membranes, described coniform hole can obtain by the mode of acupuncture.
The aperture of the coniform hole of described semi-permeable membranes is 50nm.
Described semi-permeable membranes adopts the mode of heat seal to stick on the inwall of described centrifuge tube.
Described semi-permeable membranes is selected polyvinyl chloride film or polyethylene terephthalate film.
The thickness of described semi-permeable membranes is 0.06mm.
The body of described centrifuge tube is cylindrical.
The body bottom of described centrifuge tube is conical.
Described centrifuge tube entirety is one-body molded.
The beneficial effect that the technical scheme of the utility model embodiment is brought is as follows: mononuclearcell acquisition time is foreshortened to 15min by the utility model, operation is simplified greatly, add blood sample and extract tunica albuginea layer step without careful, the pick-up rate of object cell improves greatly.
Accompanying drawing explanation
For the technical scheme of clearer explanation the utility model embodiment, below the accompanying drawing of required use during embodiment is described is briefly described, apparent, accompanying drawing in the following describes is only embodiment more of the present utility model, for those of ordinary skills, do not paying under the prerequisite of creative work, can also obtain according to these accompanying drawings other accompanying drawing.
The apparatus structure schematic diagram of a kind of separation of whole blood mononuclearcell that Fig. 1 provides for the utility model embodiment; The device of a kind of separation of whole blood mononuclearcell that Fig. 2 provides for the utility model embodiment separates mononuclearcell and separates relatively schematic diagram of mononuclearcell effect with traditional F icoll.
In figure: coniform hole 1 semi-permeable membranes 2 centrifuge tube 3 upper covers 4.
Embodiment
Below in conjunction with the accompanying drawing in the utility model embodiment; technical scheme in the utility model embodiment is carried out to clear, complete description; obvious described embodiment is only a part of embodiment of the present utility model; not whole embodiment; based on the embodiment in the utility model; those of ordinary skills are not paying the every other embodiment obtaining under creative work prerequisite, all belong to the scope of the utility model protection.
The apparatus structure schematic diagram of a kind of separation of whole blood mononuclearcell that Fig. 1 provides for the utility model embodiment.
As shown in Figure 1, comprising: centrifuge tube 3, upper cover 4, the open top of described centrifuge tube 3, closed bottom, described upper cover 4 is closely connected to the top of described centrifuge tube 3; A semi-permeable membranes 2 is installed in inside at described centrifuge tube 3, and described semi-permeable membranes 2 is for separating lymphocyte separation medium and complete blood cell, and the interior separation of described centrifuge tube 3 is become two portions by described semi-permeable membranes 2.
Uniform several coniform holes 1 on described semi-permeable membranes 2, described coniform hole 1 can obtain by the mode of acupuncture.
The aperture of the coniform hole 1 of described semi-permeable membranes 2 is 50nm.
Described semi-permeable membranes 2 adopts the mode of heat seal to stick on the inwall of described centrifuge tube 3.
Described semi-permeable membranes 2 is selected polyvinyl chloride film or polyethylene terephthalate film.
The thickness of described semi-permeable membranes 2 is 0.06mm.
The body of described centrifuge tube 3 is cylindrical.
The body bottom of described centrifuge tube 3 is conical.
Described centrifuge tube 3 entirety are one-body molded.
The utility model device separates mononuclearcell step and is described below: use PBS solution dilution whole blood, whole blood/PBS solution=3; The lymphocyte separation medium of pouring 20ml left and right in centrifuge tube 3 into, the lymphocyte separation medium of pouring into was as the criterion to just reach semi-permeable membranes 2; Use transfer pipet in centrifuge tube 3, to add the 25ml whole blood through dilution, now in pipe, volume is 45ml left and right; By centrifugal centrifuge tube 3,1500 turn 10min without slowing down without lock; After centrifugal, separator tube supernatant liquid is poured into new 50ml centrifuge tube; PBS solution washing 1500rpm 5min washing 3 times, obtains pure mononuclearcell; Use Countess calculating instrument to calculate the mononuclearcell number obtaining; The mononuclearcell number of acquisition and Ficoll method are compared as shown in Figure 2, and the device of a kind of separation of whole blood mononuclearcell that Fig. 2 provides for the utility model embodiment separates mononuclearcell and separates relatively schematic diagram of mononuclearcell effect with traditional F icoll.
The above, be only specific embodiment of the utility model, but feature of the present utility model is not limited to this, any people who is familiar with this technology is in the utility model field, the variation that can expect easily or modification, all should be encompassed in following claim of the present utility model.
Claims (9)
1. a device for separation of whole blood mononuclearcell, is characterized in that, comprises centrifuge tube, upper cover, the open top of described centrifuge tube, closed bottom, and described upper cover is closely connected to the top of described centrifuge tube; A semi-permeable membranes is installed in inside at described centrifuge tube, and the interior separation of described centrifuge tube is become two portions by described semi-permeable membranes.
2. the device of a kind of separation of whole blood mononuclearcell as claimed in claim 1, is characterized in that, uniform several coniform holes on described semi-permeable membranes.
3. the device of a kind of separation of whole blood mononuclearcell as claimed in claim 2, is characterized in that, the aperture of the coniform hole of described semi-permeable membranes is 50nm.
4. the device of a kind of separation of whole blood mononuclearcell as described in claim 1 or 2 or 3, is characterized in that, described semi-permeable membranes adopts the mode of heat seal to stick on the inwall of described centrifuge tube.
5. the device of a kind of separation of whole blood mononuclearcell as described in claim 1 or 2 or 3, is characterized in that, described semi-permeable membranes is selected polyvinyl chloride film or polyethylene terephthalate film.
6. the device of a kind of separation of whole blood mononuclearcell as claimed in claim 5, is characterized in that, the thickness of described semi-permeable membranes is 0.06mm.
7. the device of a kind of separation of whole blood mononuclearcell as described in claim 1 or 2 or 3, is characterized in that, the body of described centrifuge tube is cylindrical.
8. the device of a kind of separation of whole blood mononuclearcell as claimed in claim 7, is characterized in that, the body bottom of described centrifuge tube is conical.
9. the device of a kind of separation of whole blood mononuclearcell as claimed in claim 8, is characterized in that, described centrifuge tube entirety is one-body molded.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320832225.4U CN203602617U (en) | 2013-12-17 | 2013-12-17 | Device for separating mononuclear cells from whole blood |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320832225.4U CN203602617U (en) | 2013-12-17 | 2013-12-17 | Device for separating mononuclear cells from whole blood |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203602617U true CN203602617U (en) | 2014-05-21 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201320832225.4U Expired - Fee Related CN203602617U (en) | 2013-12-17 | 2013-12-17 | Device for separating mononuclear cells from whole blood |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203602617U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105424450A (en) * | 2015-12-17 | 2016-03-23 | 天津医科大学总医院 | Floating sphere immunofluorescent staining method and staining device |
| CN105567556A (en) * | 2016-01-22 | 2016-05-11 | 武汉海吉力生物科技有限公司 | Density gradient centrifugal tube with position-adjustable porous diaphragm and application of centrifugal tube |
| CN108587884A (en) * | 2018-07-16 | 2018-09-28 | 苏州呼呼健康科技有限公司 | A kind of centrifuge tube |
| CN110038434A (en) * | 2019-04-23 | 2019-07-23 | 卢彩婷 | A kind of assemble method of dialysis apparatus and the dialysis apparatus |
-
2013
- 2013-12-17 CN CN201320832225.4U patent/CN203602617U/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105424450A (en) * | 2015-12-17 | 2016-03-23 | 天津医科大学总医院 | Floating sphere immunofluorescent staining method and staining device |
| CN105424450B (en) * | 2015-12-17 | 2018-12-18 | 天津医科大学总医院 | Suspension cell ball immunofluorescence dyeing method and dyeing apparatus |
| CN105567556A (en) * | 2016-01-22 | 2016-05-11 | 武汉海吉力生物科技有限公司 | Density gradient centrifugal tube with position-adjustable porous diaphragm and application of centrifugal tube |
| CN105567556B (en) * | 2016-01-22 | 2017-11-03 | 武汉海吉力生物科技有限公司 | A kind of density gradient centrifugation pipe with the adjustable porous septum in position and its application |
| CN108587884A (en) * | 2018-07-16 | 2018-09-28 | 苏州呼呼健康科技有限公司 | A kind of centrifuge tube |
| CN108587884B (en) * | 2018-07-16 | 2023-07-25 | 苏州呼呼健康科技有限公司 | Centrifuge tube |
| CN110038434A (en) * | 2019-04-23 | 2019-07-23 | 卢彩婷 | A kind of assemble method of dialysis apparatus and the dialysis apparatus |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: SHANGHAI CLAISON BIOTECHNOLOGY CO., LTD. Free format text: FORMER OWNER: YE YONGQING Effective date: 20140819 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| C53 | Correction of patent for invention or patent application | ||
| CB03 | Change of inventor or designer information |
Inventor after: Ye Yongqing Inventor after: Chen Xinxi Inventor before: Ye Yongqing |
|
| COR | Change of bibliographic data |
Free format text: CORRECT: INVENTOR; FROM: YE YONGQING TO: YE YONGQING CHEN XINXI Free format text: CORRECT: ADDRESS; FROM: 350100 FUZHOU, FUJIAN PROVINCE TO: 200120 PUDONG NEW AREA, SHANGHAI |
|
| TR01 | Transfer of patent right |
Effective date of registration: 20140819 Address after: 200120 Shanghai city Pudong New Area college town right Li No. 1628 building 4 room 3072 Patentee after: Shanghai Ke Laixun Bioisystech Co., Ltd Address before: 350100, 13 F, Fu Cheng garden, 328 Hualin Road, Fujian, Fuzhou, 71D Patentee before: Ye Yongqing |
|
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140521 Termination date: 20171217 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |