CN202925017U - Sleeve tube and centrifugation container for in-vitro purifying treatment of biological samples - Google Patents
Sleeve tube and centrifugation container for in-vitro purifying treatment of biological samples Download PDFInfo
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- CN202925017U CN202925017U CN 201220665740 CN201220665740U CN202925017U CN 202925017 U CN202925017 U CN 202925017U CN 201220665740 CN201220665740 CN 201220665740 CN 201220665740 U CN201220665740 U CN 201220665740U CN 202925017 U CN202925017 U CN 202925017U
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Abstract
The utility model relates to a sleeve tube and centrifugation container for the in-vitro purifying treatment of biological samples. The sleeve tube comprises a tube body, movable blocking plates, a connecting shaft, a sealing ring and a coaxial lock switch, wherein the tube body is a cylinder-shaped one, the upper end of the tube body is provided with a flange, the bottom end face of the tube body is provided with first openings, and the bottom end of the sidewall of the tube body is provided with second openings and bulgy blocks; the sealing ring is sleeved at the outer side of the second openings; the movable blocking plates are arranged at the bottom of the tube body, are annular-fan-shaped ones and are provided with third openings, and outer edges of the movable blocking plates are in one-to-one correspondence to the second openings and are in contact with the sealing ring; the connecting shaft is located at the central position of the tube body, the top end of the connecting shaft is connected with the coaxial lock switch, the bottom end of the connecting shaft is provided with a polygonal component, and the sides of the polygonal component are in one-to-one correspondence to inner edges of the movable blocking plates; and the coaxial lock switch is located on the upper surface of the flange. The utility model further provides a centrifugation container comprising the sleeve tube. The sleeve tube and the centrifugation container, provided by the utility model, are applied to the in-vitro treatment of biological samples and have the advantages of convenience, practicality, low production cost, simple production process, safety and no toxicity.
Description
Technical field
The utility model relates to a kind of sleeve pipe and centrifuge container of processing for the biological specimen purification, belongs to the biological medicine technology field.
Background technology
Utilizing the biological particles in centrifuging ex vivo enrichment biological specimen is a technology that is most widely used in biological study.Many biological particless all can be realized enrichment by this technology of centrifuging, for example, and the cell of animal or plant, subcellular structure, bacterium, virus or biomacromolecule etc.These performances under the action of the centrifugal of biological particles in suspension depend primarily on size and the density of biological particles, and this can be explained by Stokes equations (Stokes equation).Stokes equations (seeing following formula) has been described the Changing Pattern of movement rate when spheroidal particle is subject to centrifugation in liquid medium:
Wherein: d represents the diameter of spheroidal particle, p
2Represent the density of spheroidal particle, p
1Represent the density of liquid medium, n represents the viscosity of liquid medium, and g represents centrifugal force.By Stokes equations as can be known, when the equal density of particle and liquid medium, the subsidence rate of particle in centrifugal field is zero, and this is the theoretical basis of utilizing centrifuging enrichment particular organisms particle.For example, when utilizing the lymphocyte in centrifuging ex vivo enrichment human blood sample, because the density of human lymphocyte is about 1.077g/cm
3, can utilize density to be all 1.077g/cm
3Density medium realize this process of lymphocyte-rich from the human blood sample; And for example, the density of DNA is at 1.500-1.800g/cm
3Between, should select the raw materials such as the cesium chloride that can be mixed with this density range solution or cesium sulfate as the density medium formula.
Utilize centrifugal principle to carry out the biological specimen purification and process, namely the step of enriched biological particle is substantially similar from biological specimen.For example, when utilizing density medium to carry out the ex vivo enrichment of human lymphocyte in blood, its general operation steps comprises: dilute blood sample in advance with a certain proportion of balanced salt solution or physiological saline (1); (2) blood sample after diluting adds in the centrifuge container that is preinstalled with density medium along the centrifuge container wall carefully, keep the interface of sample and medium clear, do not mix, this process is the committed step that guarantees concentration effect, often is called as " paving blood "; (3) carry out centrifugally under certain temperature and certain centrifugal condition, for example 20 ℃ the time, centrifugal force is under the condition of 400g centrifugal 30 minutes; (4) the careful centrifuge container that sample is housed that takes out from whizzer utilizes the devices absorptions such as pasteur pipet or transfer pipet to be enriched in " the tunica albuginea layer " at sample and medium layer interface place; (5) cellular product that obtains with a certain proportion of balanced salt solution, physiological saline or cell culture medium washing is removed thrombocyte, cell debris and part dead cell, and remaining density medium; (6) cell suspension that obtains is used for the subsequent experimental such as cell cultures.
For realizing good concentration effect, the aforesaid operations step is very high for operator's technical requirements.For example, in the operation of above-mentioned steps (2), the operator must slowly be layered on the blood sample after dilution on density medium very carefully, keeps the interface of sample and medium clear, does not mix.This be because, if blood sample mixes with density medium, " the tunica albuginea layer " that finally be enriched between sample and medium layer will become very loose, and contain a large amount of red blood cell contaminations, this operates the obstacle that brings on visual determination not only can for the collection in step (4), also can produce serious detrimentally affect to yield and the purity of purpose cell.Therefore, the characteristics of " paving blood " this operation are not only time-consuming, but also efforts, and are high for operator's technical requirements, are unfavorable for realizing that mass-producing processes, and during especially for the clinical treatment purposes, it is a large technical barrier that the mass-producing of a large amount of blood samples is processed.again for example, in the operation of above-mentioned steps (4), at first the operator needs the position of " tunica albuginea layer " is accurately judged, then utilize the devices such as pasteur pipet or transfer pipet to stretch into following absorption of liquid level and be enriched in this regional purpose cell, this not only wastes time and energy, and along with the purpose cell constantly is sucked out, the thickness of tunica albuginea layer dwindles gradually, outward appearance and position are also more and more not obvious, this has proposed higher requirement again for the judgement when operator should stop suction operation, if the operator is to the error in the surplus appearance judgement of cell, stopped in advance suction operation, the yield of purpose cell will obviously reduce.
Based on the problem in aforesaid operations, some solutions are proposed in succession.For example, some overseas enterprises have developed and have been specifically designed to the lymphocytic centrifuge tube of purifying from blood sample, the representational product that comprises the exploitation of German Greinerbio-one company
Centrifuge tube, the characteristics of this centrifuge tube are to omit the bottom installation to have fixed one deck porous filter screen barrier in pipe, and centrifuge tube is divided for two compartments in up and down.Because the aperture of barrier is smaller, blood sample can't see through barrier at once, blood sample can be poured directly into like this in the centrifuge tube that lower compartment is preinstalled with density medium, and needn't worry that blood sample mixes with density medium.In follow-up centrifugally operated, due to the radius of the hemocyte aperture much smaller than barrier, so hemocyte can freely pass through screen barrier and contact with medium layer, and do not affect layered effect.Such device has solved the difficulty in " paving blood " operation described in above-mentioned steps (2) preferably, has greatly shortened the operating time, has improved working efficiency and effect.Yet, this defective with centrifuge tube of porous filter screen barrier structure is also fairly obvious, particularly comprise following some: (a) because the aperture of barrier is smaller, not only blood sample can't see through barrier at once, density medium equally also can't see through barrier at once, therefore the standard operation that is applicable to this device be first upwards compartment add appropriate density medium, such as Ficoll-Paque
TMPlus, then centrifugal by several minutes, make density medium see through barrier and all enter lower compartment, can carry out follow-up " paving blood " operation, and this has introduced extra trouble again for the lock out operation of blood sample.In addition, the amount of the density medium that adds need to obtain controlling very accurately, and it should a little less than the height of barrier, if add-on is very few, will have a negative impact to purification effect; If add-on is too much, also need to reclaim the density medium over barrier height, this is similarly lock out operation and has brought inconvenience.(b) have the production technique more complicated of the centrifuge tube of porous filter screen barrier structure, only external several enterprises can complete process for processing at present, and domestic shortage proven technique and corresponding product.This is main because installing fixedly on smooth centrifugal tube wall, one deck porous filter screen barrier structure has proposed very high requirement to adhering technique.Bondingly not only need enough physical strengths to guarantee that in transportation and centrifugally operated, barrier can not come off, the more important thing is, that the selection of tackiness agent need to be considered is water insoluble, to Biosafety sexual factors such as cell are nontoxic.The problem of tackiness agent is transformed for the potential clinical application of this series products and has brought huge obstruction.(c) based on the complicacy of technical matters, this product involves great expense, and it is approximately tens times to 100 times of same capacity centrifuge tube price that every brace has the centrifuge tube of porous filter screen barrier structure, and this has limited being widely used of this series products greatly.
For addressing the aforementioned drawbacks the problem described in (a), U.S. Sigma company has developed product A CCUSPIN
TMSystem-
-1077, it is a kind of centrifuge tube with porous filter screen barrier structure that is preinstalled with appropriate density medium.The solution of prepackage density medium is really fine has solved the problem described in defects (a), yet has brought again defective as described below: (d) storing mode of this series products must be set separately for density medium.ACCU SPIN
TMSystem-
Explicitly point out in-1077 specification sheets, this centrifuge tube with porous filter screen barrier structure that is preinstalled with appropriate density medium must be preserved under 2-8 ℃, lucifuge condition, the volume shared due to centrifuge tube is larger, and the application that this special preserving type is this series products has brought very big inconvenience.(e) under the long-term immersion of water-based density medium, whether the adhesive effect of screen barrier and result of extraction can also satisfy service requirements lacks sufficient testing data support.To sum up, (b), (c) described problem during the centrifuge tube with porous filter screen barrier structure that is preinstalled with appropriate density medium not only can't address the aforementioned drawbacks, also additionally introduced defective (d) and (e) described in the problem of preservation, Product Safety and validity.
Chinese patent 200610063125.4 provides a kind of and has been provided with the separation of lymphocytes pipe of loose porous material at the pipe end, this loose porous material can become semi-solid state with density medium absorption, when carrying out the purifying of blood sample, can directly pour blood sample into this separator tube, and needn't worry to mix up blood and parting liquid interface both, finely solved the problem that " paving blood " operation is wasted time and energy.And owing to can realize fixing by frictional force between described loose porous material and body, thereby this technology do not need to use tackiness agent, technology difficulty reduces greatly, thus overcome (b) in the defects and (c) described in problem.Yet, this separation of lymphocytes pipe that is provided with loose porous material at the pipe end still faces the problem consuming time of density medium adition process, because can stoping blood, loose porous material sees through at once, the absorption of density medium and infiltration also need certain hour, need could realize by " dropping "; And because loose porous material itself is not transparent, the add-on of density medium also more difficult realization is accurately controlled, this so that brought very large difficulty for the control of blood sample separating effect and the repeatability of result.Above-mentioned patent also provides another kind of product form, namely in advance lymphocyte separation medium (density medium) is added in the loose porous material of body, although this can realize the accurate control of " plug and play " and density medium add-on, yet, the material of loose porous material be faced with equally defects (d) and (e) described in the problem of preservation, Product Safety and validity.Namely, the density medium that adds in advance with long period of soaking in loose porous material, whether the meeting stripping is for the harmful composition of hemocyte under this soaking conditions for loose porous material (the extraordinary sponge of for example putting down in writing in above-mentioned patent), and this need to further prove by lot of experiments.
The utility model content
For solving the problems of the technologies described above; the purpose of this utility model is to provide a kind of sleeve pipe for the processing of biological specimen purification; this sleeve pipe can be packed among centrifuge container; process with the purification that is used for biological specimen; that this sleeve pipe has is convenient and practical, production technique is simple, cost is low and the characteristics of safety non-toxic; and the mass-producing that is particularly suitable for biological specimen processes, and makes operating process obtain the simplification of very big degree.
The purpose of this utility model also is to provide a kind of centrifuge container that comprises above-mentioned sleeve pipe.
For achieving the above object, at first utility model of the present invention provides a kind of sleeve pipe for the processing of biological specimen purification, it is characterized in that, should be used for sleeve pipe that the biological specimen purification processes and comprise body, some shifting boards, coupling shaft, sealing-ring, coaxial locking key, wherein:
Described body is tubular, its upper end has export-oriented flange, and its bottom end face is provided with several the first openings, and the bottom of its sidewall is provided with several second openings and several export-oriented projections, wherein, described projection is positioned at the top of the connection section of adjacent two described the second openings;
Described sealing-ring is placed in the outside of described the second opening part;
Described shifting board is located at the bottom of described body, which is provided with several the 3rd openings, and the outer rim of described shifting board and described the second opening are corresponding and contact with described sealing-ring one by one;
Described coupling shaft is positioned at the central position of described body, and its top is connected with described coaxial locking key, and the bottom is provided with Polygons parts, each limit of these Polygons parts and the corresponding and contact one by one of the inner edge of a described shifting board;
Described coaxial locking key is positioned on the upper surface of flange of described body and can rotates along this upper surface;
When described shifting board is in open mode, at least part of coincidence between described the first opening and the 3rd opening; When described shifting board is in closure state, there is not the part of coincidence between described the first opening and the 3rd opening, the shape and size of described the second opening and the outer rim of described shifting board are complementary.
At above-mentioned sleeve pipe for the processing of biological specimen purification, preferably, described the second opening is rectangle.
At above-mentioned sleeve pipe for the processing of biological specimen purification, preferably, the thickness of described sealing-ring is identical with the height of described the second opening.
At above-mentioned sleeve pipe for the processing of biological specimen purification, preferably, described sealing-ring is rubber ring.
At above-mentioned sleeve pipe for the processing of biological specimen purification, preferably, described shifting board is fan-shaped for ring, and, the thickness of described shifting board is identical with the thickness of described Polygons parts, and all shifting boards and described Polygons parts form a complete disc; Perhaps described shifting board is fan-shaped, the thickness of the centre portions of described baffle plate is lower than the thickness of Outboard Sections, and, the thickness sum of the thickness of the centre portions of described shifting board and described Polygons parts is equal to or greater than the thickness of described shifting board Outboard Sections, and all shifting boards and described Polygons parts form a complete disc.For ring when fan-shaped, its inner rim can be straight flange when shifting board, and all shifting boards form the disk that a centre has the Polygons cavity, and this Polygons cavity is identical with the shape of the Polygons parts of coupling shaft bottom; When shifting board when being fan-shaped, its centre portions outside half part thinner, the centre portions of all shifting boards is combined together to form a reeded disk in centre, the limit of this groove is preferably linear pattern, its shape and Polygons parts can mate mutually, the Polygons parts also slightly thinner in case with the thickness matching of shifting board, by the middle portion with shifting board be pressed in the Polygons parts below, can make shifting board be arranged on preferably sleeve bottom, perk can not occur, in order to avoid affect sealing effectiveness.
At above-mentioned sleeve pipe for the processing of biological specimen purification, preferably, the sleeve pipe that should be used for the processing of biological specimen purification comprises 3-12 shifting board, and the limit number of the quantity of described the second opening, the quantity of described projection, described Polygons parts is identical with the quantity of described shifting board.More preferably, above-mentioned sleeve pipe comprises 3-6 shifting board.
At the above-mentioned sleeve pipe of processing for the biological specimen purification, preferably, the outer edge side mask of described shifting board has a groove that is complementary with sealing-ring.Sealing-ring can be enclosed within this groove, keeps stability, to avoid slippage.
At above-mentioned sleeve pipe for the processing of biological specimen purification, preferably, the flange of described sleeve pipe is provided with at least two to (four) lock slots, described lock slots is relative in twos, described coaxial locking key comprises a cross bar, be connected and can drive the coupling shaft rotation with the coupling shaft top in the middle of this cross bar, its two ends also can be changed between different lock slots among laying respectively at the pair of locking groove.
The utility model also provides a kind of centrifuge container for the processing of biological specimen purification, it is characterized in that, the centrifuge container that should be used for the processing of biological specimen purification comprises above-mentioned sleeve pipe and centrifuge container body for the processing of biological specimen purification, wherein, the described sleeve pipe of processing for the biological specimen purification be positioned among described centrifuge container body and both movable sleeve join.
Above-mentioned for the biological specimen purification process centrifugal, preferably, when described shifting board was in open mode, the expansion of described sealing-ring also can be together with the projection of tube wall bottom with the gap sealing between described centrifuge container body and described sleeve pipe.
The above-mentioned sleeve pipe that the utility model provides is for the centrifuge container of packing into (for example common centrifuge tube, centrifugal bottle), so that realization is to the enrichment of the biological particles in biological specimen.For ease of sleeve pipe being put into centrifuge container or it being taken out, the external diameter of this sleeve pipe body is slightly less than the internal diameter of centrifuge container, and both movable sleeve is joined, and the length of this sleeve pipe (or claiming height) can be determined in conjunction with the size of centrifuge container as required.The size of the flange at this sleeve pipe top to be can make casing hanger be fixed on centrifuge container, and the use that does not affect the centrifuge container lid is advisable, and preferably is equal to or slightly less than the full diameter of thread of centrifuge container.The lateral dimension (external diameter or with respect to the height of tube wall) that is arranged in the projection of sleeve pipe body bottom should make this projection can comparatively closely contact with the centrifuge container inwall, make again sleeve pipe can put into smoothly centrifuge container and take out, preferably, the internal diameter of the diameter of the circle that forms of projection outer rim and centrifuge container is identical or be slightly less than its internal diameter.In addition, the bottom surface of this projection should be in contact with one another with sealing-ring, avoid it to upwarp in order to can push down sealing-ring, simultaneously, when shifting board is in open mode, this projection also can be pushed down the outer of shifting board and avoid it to upwarp, and the part gap sealing that produces after can also together with rubber ring, shifting board being opened is lived.
In above-mentioned sleeve pipe, shifting board is arranged on the bottom of body, and can be along radial direction free movement within the specific limits.The outer rim of shifting board is arc, linking together to form a circle.The biological particles in biological specimen all is provided with the 3rd opening of some amount on each piece shifting board, so that can pass through.Shifting board has the open and close two states, when shifting board is in closure state, whole shifting boards forms the ring-type of a sealing, outer rim connects together and forms the circle of a sealing, and, the Polygons cavity at center or Polygons groove are just in time filled by the Polygons parts of coupling shaft bottom, and thus, shifting board forms with the Polygons parts of coupling shaft bottom circle or the disk that seals.According to specific embodiments of the present invention, preferably, sleeve pipe provided by the invention comprises 3-12 (preferred 3-6) shifting board, and the limit number of the quantity of the second opening, the quantity of projection, Polygons parts is identical with the quantity of shifting board.Corresponding to 3-12 shifting board, the Polygons parts of coupling shaft bottom can be equilateral triangle-dodecagon, and corresponding to 3-6 shifting board, the Polygons parts bottom coupling shaft can be equilateral triangle-regular hexagon.
The first opening of offering on the body bottom end face of sleeve pipe be for the biological particles that makes biological specimen (for example hemocyte) can be freely in centrifugal field by and contact with density medium in being contained in centrifuge container.The shape of this first opening and size can be selected as required, but will with shifting board on the 3rd opening adapt, in order to biological specimen can be contacted with density medium with the intersection of the 3rd opening by the first opening.According to specific embodiments of the present invention, preferably, when shifting board was in open mode, at least part of coincidence between the first opening and the 3rd opening (was preferably fully and overlaps, in such cases, the first opening of correspondence position and the 3rd opening can be of similar shape); When shifting board is in closure state, there is not the part of coincidence between the first opening and the 3rd opening, i.e. sealing mutually.
The second opening that arranges on the sidewall of sleeve pipe can arrange as required, its quantity should be consistent with the quantity of shifting board, and, this second opening should make shifting board can freely come in and go out and satisfy certain sealing needs when open mode, and namely the size of the second opening and shape should be complementary with physical dimension and the shape of shifting board.The quantity of the projection that arranges on the tube wall also quantity with shifting board is identical.Above-mentioned the second opening is along the outer wall setting of sleeve pipe, and it can be rectangle (or rectangle of title curved surface), and namely bottom and upper segment is parallel with the bottom end face of sleeve pipe respectively, and the bottom end face with sleeve pipe is vertical respectively with the right on the left side.
In sleeve pipe provided by the invention, the outside that sealing-ring is sheathed on the second opening is used in use to the sealing gap between sleeve pipe and centrifuge container.The sealing circle can be any circle of sealing can realized, is preferably resilient sealing-ring, for example rubber ring.The internal diameter of sealing circle can be identical with the external diameter of body, so that sheathed.The inner edge of sealing circle contacts with the outer rim of shifting board, and the outer rim of each shifting board connects together the circular diameter that forms should be identical with the internal diameter of sealing-ring.
in sleeve pipe provided by the invention, when shifting board is in closed state, it is in " contraction " state, sealing-ring also is in " contraction " state (there is no elastically-deformable state), its external diameter is slightly less than the internal diameter of centrifuge container, gap between sleeve pipe body and centrifuge container can be not sealed, the sleeve pipe body can freely take out from centrifuge container or put into, the 3rd opening on shifting board and the first opening bottom body block by differing mutually to each other at this moment, body is in relatively closed state, and biological specimen can not pass through, and when shifting board is in open mode, it is in " expansion " state, first opening of the 3rd opening on shifting board bottom body be at least part of the coincidence each other, biological specimen can freely pass through, simultaneously, sealing-ring is also along with the expansion of shifting board, edge by shifting board is outwards pushed along radial direction, become " expansion " state and with centrifuge container outer wall close contact, and together with the projection of body bottom with the gap sealing between sleeve pipe body and centrifuge container inwall (this moment, the external diameter of sealing-ring reached the size of centrifuge container internal diameter), biological specimen can not pass through, even also can't pass through this gap in centrifugal field.
In above-mentioned sleeve pipe, the Polygons parts of coupling shaft bottom and shifting board by the central side close contact, it is that rotational synchronization by Polygons parts, coupling shaft and coaxial locking key realizes that the state of shifting board switches (open and close).Correspondingly, the Polygons parts also can have two kinds of use states of open and close, and (description of open and close herein defines for the state with shifting board is consistent, and the shape that does not mean that Polygons parts itself will be opened or closure), when the Polygons parts are in closure state, the edge of Polygons parts and shifting board by the parallel contact in central side edge, shifting board is in closure state, and sealing-ring shrinks, and body is in relative closure state; When the Polygons parts are in open mode, the summit of Polygons parts and shifting board by the central side edge contact, shifting board outwards produces the displacement of certain distance along radial direction, first opening of the 3rd opening on shifting board bottom body be at least part of the coincidence each other, forwards open mode to by closure state.
In above-mentioned sleeve pipe, coupling shaft be positioned at body the center, overlap with the central shaft of body, its top is connected with coaxial locking key.Coaxial locking key can rotate by rotarily driving coupling shaft, thereby drives the location status that changes the Polygons parts, and then realizes the switching of shifting board between closure and open mode.When shifting board is under closed two kinds of location status with opening, coaxial locking key all can be realized avoiding shifting board unexpected displacement occuring in centrifugal field or under the carrying state at the locking position of body upper end annular flange flange by certain latch-up structure.Above-mentioned latch-up structure can be any device that can realize above-mentioned locking, preferably, the flange of sleeve pipe is provided with at least two to (four) lock slots, coaxial locking key comprises a cross bar, be connected and can drive the coupling shaft rotation with the coupling shaft top in the middle of this cross bar, its two ends also can be changed between different lock slots among laying respectively at the pair of locking groove.For example, four lock slots (groove) are set on the flange of sleeve pipe, make it relative in twos, is connected with the coupling shaft top in the middle of the cross bar of coaxial latch-up structure and also can drives the coupling shaft rotation, its two ends lay respectively among the pair of locking groove, this cross bar can turn in lower a pair of lock slots under external force, can realize the conversion of shifting board between the opening and closing state by rotating.The shape of cross bar is as long as be fit to the lock slots shape, and the size of cross bar is suitable with the size of lock slots, makes cross bar that certain resistance be arranged when falling into lock slots, guarantees that cross bar is fixed by resistance in a longitudinal direction; The two ends of this cross bar can comprise two bar heads that make progress, so that apply the external force rotating horizontal pole and cross bar two ends applied two power at sensing center, make the perk of cross bar two break away from the pair of locking groove, and keep cross bar can slide into along the flange of sleeve pipe when two perk state another to lock slots in; Cross bar is in lock slots the time, and it can be locked on turning direction, with the position and the state locking that realize shifting board, guarantee that shifting board can not open arbitrarily; Simultaneously, due to cross bar in a longitudinal direction by and lock slots between resistance be fixed, whole coupling shaft can be not up and down together with the Polygons parts, realized in a longitudinal direction locking yet., cross bar is temporarily spun off from lock slots and forward another to locked in lock slots in the cross bar two ends and when reaching a certain size when a pair of External Force Acting that points to the center, this cross bar preferably has certain elasticity, so that by roll-in and roll-out; Distance between the two pairs of lock slots can determine according to the quantity of shifting board, as long as can realize conversion and locking between the open and close state of shifting board.
The material that sleeve pipe provided by the utility model adopts can be any medical material that satisfies relevant hygienic standard, for example the material of this sleeve pipe can be the plastic material of the syringe used for the preparation of clinical injection, the elastomeric material of the rubber ring that sealing is adopted can be the medical grade rubber that meets hygienic standard, for example be used for to make the elastomeric material of the rubber components of the injector push-rod leading portion that clinical injection uses, such as medical grade silicon rubber etc.
The sleeve pipe that the utility model provides and centrifuge container can be used for the biological particles of biological specimen is carried out enrichment, above-mentioned biological specimen is blood or tissue samples etc., and the biological particles in biological specimen is lymphocyte, monocyte, hemopoietic stem cell, mescenchymal stem cell etc.; Specifically can carry out according to following steps:
Sleeve pipe is assemblied in centrifuge container, and opens the shifting board of sleeve pipe;
Density medium is packed in the centrifuge container body, close the shifting board of sleeve pipe;
In the biological specimen sprue bushing;
Open the shifting board of sleeve pipe, biological specimen is contacted with density medium, carry out centrifugal treating, form the biological particles enriched layer after centrifugal treating above the shifting board of sleeve pipe;
Close the shifting board of sleeve pipe, sleeve pipe is taken out from the centrifuge container body, the biological particles enriched layer is shifted, complete the enrichment of biological particles in biological specimen.
Separating by aforesaid method the biological particles enriched layer that obtains can be by being used for different purposes after suitable processing, concrete processing can be carried out according to usual manner.
In aforesaid method, the amount of the density medium of packing into can determine as required, and preferably, the upper surface of the density medium in the centrifuge container body of packing into contacts with the bottom face of sleeve pipe or a little more than bottom face.
Sleeve pipe provided by the utility model is for density gradient centrifugation existing problem and proposing during the enriched biological particle from the biological specimens such as blood or tissue samples, and can solve " paving blood " operation and " drawing the tunica albuginea layer " operation wastes time and energy and be unfavorable for that mass-producing processes the problem of biological specimen; The currently available products that provides in background technology or the defective described in technology existing (a)-(e) also can be provided.Sleeve pipe provided by the utility model is the device that the biological specimen purification is processed that is used for of the simple and safety non-toxic of a kind of convenient and practical, low production cost, production technique.
Description of drawings
The following drawings only is intended to the utility model is done and schematically illustrates and explain, does not limit scope of the present utility model.Wherein:
The one-piece construction schematic diagram of the sleeve pipe that Fig. 1 provides for embodiment 1;
The shifting board of the sleeve pipe that Fig. 2 provides for embodiment 1 and the exploded perspective view of coupling shaft;
The closure state schematic diagram of the shifting board of the sleeve pipe that Fig. 3 provides for embodiment 1;
The open mode schematic diagram of the shifting board of the sleeve pipe that Fig. 4 provides for embodiment 1;
The first opening schematic diagram of the sleeve bottom end face that Fig. 5 provides for embodiment 1;
The second opening and the projection schematic diagram of Fig. 6 provides for embodiment 1 sleeve pipe side;
Fig. 7 is shifting board in embodiment 2 and the structural representation of Polygons parts;
Fig. 8 is the set-up mode schematic diagram of coaxial locking key in embodiment 1;
Fig. 9 is that the A-A of Fig. 8 is to sectional view.
Main drawing reference numeral explanation:
Embodiment
Understand for technical characterictic of the present utility model, purpose and beneficial effect being had more clearly, referring now to Figure of description, the technical solution of the utility model is carried out following detailed description, but but can not be interpreted as restriction to practical range of the present utility model.
The present embodiment provides a kind of sleeve pipe of processing for the biological specimen purification, and it comprises body 1, six shifting boards 2, coupling shaft 3, sealing-ring 4, coaxial locking key 5, as shown in Figure 1, and wherein:
The upper end of body 1 has export-oriented flange 6, the bottom end face of body 1 is provided with several the first openings 7 (as shown in Figure 5), the bottom of its sidewall is provided with several second openings 9 and several export-oriented projections 8, wherein, projection 8 is positioned at the top (as shown in Figure 6) of the connection section 12 of adjacent two the second openings 9;
Sealing-ring 4 is a rubber ring, is placed in the outside at the second opening 9 places;
Shifting board 2 is located at the bottom of body 1, and it is fan-shaped for ring, which is provided with several the 3rd openings 10, and the outer rim of this shifting board 2 and the second opening 9 are corresponding and contact (as shown in Figure 2, Figure 3, Figure 4) with sealing-ring 4 one by one;
Coupling shaft 3 is positioned at the central position of body 1, its top is connected with coaxial locking key 5, the bottom is provided with Polygons parts 11 (being in the present embodiment regular hexagon), each limit of these Polygons parts 11 and the corresponding and contact (as shown in Fig. 2,3) one by one of the inner edge of a shifting board 2;
Coaxial locking key 5 is positioned on the upper surface of flange 6 of body 1 and can rotates along this upper surface;
this coaxial locking key 5 comprises a cross bar 13 and is located at the bar head 14 at cross bar 13 two ends, as Fig. 8 and shown in Figure 9, be provided with two to (four) lock slots 15 on the flange 6 of body 1 upper end, relative in twos, the two ends of cross bar 13 are positioned at wherein among pair of locking groove 15 and are locked, this moment, shifting board 2 was in open mode, when a pair of power at the sensing center that bar head 14 is applied a certain size, having certain elastic cross bar 13 breaks away from from this a pair of lock slots 15, keep this to power and the bar head applied tangential external force making cross bar 13 forward another to among lock slots 15 and cross bar 13 is fallen into and locked, cross bar 13 can drive coupling shaft 3 when rotating, Polygons parts 11 rotate together, finally make shifting board 2 outwards produce certain displacement along radial direction, it forwards closure state to by open mode.
The present embodiment provides a kind of sleeve pipe for the processing of biological specimen purification, wherein, the structure of shifting board 2 and Polygons parts 11 as shown in Figure 7, shifting board 2 comprises centre portions and Outboard Sections, the thickness of its centre portions is lower than Outboard Sections, when shifting board 2 is combined, can form a reeded disk in centre; The outer edge mask of this shifting board 2 has a groove, and the size match of this groove and sealing-ring 4 can make sealing-ring 4 covers in the above, prevents slippage and displacement;
And Polygons parts 11 are also thinner with respect to the Polygons parts 11 in embodiment 1, and its size just in time is complementary with the groove that shifting board 2 is combined in the middle of the disk of formation, and other structures of this sleeve pipe are identical with embodiment 1.
The present embodiment provides a kind of centrifuge tube for the processing of biological specimen purification, and it comprises the sleeve pipe that a common centrifuge tube body and embodiment 1 or 2 provide, and this sleeve pipe is positioned among the centrifuge tube body and with its movable sleeve joins.
shifting board 2 has closed and opens two states, respectively as shown in Figure 3 and Figure 4, wherein: when being in closure state, six shifting boards 2 mutually combine with Polygons parts 11 and form a circle, sealing-ring 4 also is in contraction schedule at this moment, be enclosed within the outside of body 1 second opening 9 and contact with the outer rim of shifting board 2, the first opening 7 of body 1 bottom and the 3rd opening 10 on shifting board 2 do not exist and overlap the zone, and body 1 is in the state of a relative closure, by rotating coaxial locking key 5, can drive coupling shaft 3 and 11 rotations of Polygons parts, when Polygons parts 11 rotate, its side can promote shifting board 2 it is outwards moved along radial direction, be transformed into open mode by closure state, under this state, shifting board 2 with sealing-ring 4 to extrapolation, make sealing-ring 4 expand and touch the inwall of centrifuge tube body, with the gap sealing between sleeve pipe and centrifuge tube body, the first opening 7 of body 1 bottom and the 3rd opening 10 on shifting board 2 overlap, body 1 is in the state of an opening, the liquid that can allow to be positioned at both sides, box face up and down is in contact with one another.
The centrifuge tube that the present embodiment provides can carry out enrichment to the mononuclearcell in blood sample according to following steps:
Sleeve pipe is placed in the centrifuge tube body interior, opens the shifting board 2 of sleeve pipe;
Density medium is packed in the centrifuge tube body, and the liquid level of loading port density medium reaches the height that contacts with the casing shoe face, closes the shifting board 2 of sleeve pipe;
Blood sample is poured directly in sleeve pipe, need not carefully to inject along internal surface of sleeve pipe;
Open the shifting board 2 of sleeve pipe, blood sample is contacted with density medium, carry out centrifugal treating under 20 ℃, the condition of 400g centrifugal force, after centrifugal 30 minutes, form tunica albuginea layer, i.e. mononuclearcell layer above the shifting board 2 of sleeve pipe;
Close the shifting board 2 of sleeve pipe, sleeve pipe is taken out from the centrifuge tube body, the plasma layer that is rich in thrombocyte and cell debris is shifted out with pasteur pipet or pipette, extract, and the tunica albuginea layer is shifted, complete the enrichment of the mononuclearcell in blood sample.
Claims (10)
1. one kind is used for the sleeve pipe that the biological specimen purification is processed, and it is characterized in that, and sleeve pipe that the biological specimen purification processes be should be used for and body, some shifting boards, coupling shaft, sealing-ring, coaxial locking key comprised, wherein:
Described body is tubular, its upper end has export-oriented flange, and its bottom end face is provided with several the first openings, and the bottom of its sidewall is provided with several second openings and several export-oriented projections, wherein, described projection is positioned at the top of the connection section of adjacent two described the second openings;
Described sealing-ring is placed in the outside of described the second opening part;
Described shifting board is located at the bottom of described body, which is provided with several the 3rd openings, and the outer rim of described shifting board and described the second opening are corresponding and contact with described sealing-ring one by one;
Described coupling shaft is positioned at the central position of described body, and its top is connected with described coaxial locking key, and the bottom is provided with Polygons parts, each limit of these Polygons parts and the corresponding and contact one by one of the inner edge of a described shifting board;
Described coaxial locking key is positioned on the upper surface of flange of described body and can rotates along this upper surface;
When described shifting board is in open mode, at least part of coincidence between described the first opening and the 3rd opening; When described shifting board is in closure state, there is not the part of coincidence between described the first opening and the 3rd opening, the shape and size of described the second opening and the outer rim of described shifting board are complementary.
2. the sleeve pipe for the processing of biological specimen purification according to claim 1, is characterized in that, described the second opening is rectangle.
3. the sleeve pipe for the processing of biological specimen purification according to claim 1, is characterized in that, the thickness of described sealing-ring is identical with the height of described the second opening.
4. the sleeve pipe for the processing of biological specimen purification according to claim 3, is characterized in that, described sealing-ring is rubber ring.
5. the sleeve pipe of processing for the biological specimen purification according to claim 1, it is characterized in that, described shifting board is fan-shaped for ring, and, the thickness of described shifting board is identical with the thickness of described Polygons parts, and all shifting boards and described Polygons parts form a complete disc; Perhaps described shifting board is fan-shaped, the thickness of the centre portions of described baffle plate is lower than the thickness of Outboard Sections, and, the thickness sum of the thickness of the centre portions of described shifting board and described Polygons parts is equal to or greater than the thickness of described shifting board Outboard Sections, and all shifting boards and described Polygons parts form a complete disc.
6. the sleeve pipe of processing for the biological specimen purification according to claim 1, it is characterized in that, the sleeve pipe that should be used for the processing of biological specimen purification comprises 3-12 shifting board, and the limit number of the quantity of described the second opening, the quantity of described projection, described Polygons parts is identical with the quantity of described shifting board.
7. the sleeve pipe of processing for the biological specimen purification according to claim 5, is characterized in that, the outer edge side mask of described shifting board has a groove that is complementary with described sealing-ring.
8. the sleeve pipe of processing for the biological specimen purification according to claim 1, it is characterized in that, the flange of described sleeve pipe is provided with at least two pairs of lock slots, described coaxial locking key comprises a cross bar, be connected with the coupling shaft top in the middle of this cross bar, its two ends also can be changed between different lock slots among laying respectively at the pair of locking groove.
9. one kind is used for the centrifuge container that the biological specimen purification is processed, it is characterized in that, the centrifuge container that should be used for the processing of biological specimen purification comprises sleeve pipe and the centrifuge container body for the processing of biological specimen purification claimed in claim 1, wherein, the described sleeve pipe of processing for the biological specimen purification be positioned among described centrifuge container body and both movable sleeve join.
10. the centrifuge container of processing for the biological specimen purification according to claim 9, it is characterized in that, when described shifting board was in open mode, described sealing-ring expansion also can be with the gap sealing between described centrifuge container body and described sleeve pipe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220665740 CN202925017U (en) | 2012-12-05 | 2012-12-05 | Sleeve tube and centrifugation container for in-vitro purifying treatment of biological samples |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220665740 CN202925017U (en) | 2012-12-05 | 2012-12-05 | Sleeve tube and centrifugation container for in-vitro purifying treatment of biological samples |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202925017U true CN202925017U (en) | 2013-05-08 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201220665740 Expired - Lifetime CN202925017U (en) | 2012-12-05 | 2012-12-05 | Sleeve tube and centrifugation container for in-vitro purifying treatment of biological samples |
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| Country | Link |
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| CN (1) | CN202925017U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103849562A (en) * | 2012-12-05 | 2014-06-11 | 北京东方华辉生物医药科技有限公司 | Sleeve for biological sample in-vitro purification treatment |
-
2012
- 2012-12-05 CN CN 201220665740 patent/CN202925017U/en not_active Expired - Lifetime
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103849562A (en) * | 2012-12-05 | 2014-06-11 | 北京东方华辉生物医药科技有限公司 | Sleeve for biological sample in-vitro purification treatment |
| CN103849562B (en) * | 2012-12-05 | 2015-06-10 | 北京东方华辉生物医药科技有限公司 | Sleeve for biological sample in-vitro purification treatment |
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