CN202543220U - High-throughput cell culture incubator - Google Patents
High-throughput cell culture incubator Download PDFInfo
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- CN202543220U CN202543220U CN 201220167162 CN201220167162U CN202543220U CN 202543220 U CN202543220 U CN 202543220U CN 201220167162 CN201220167162 CN 201220167162 CN 201220167162 U CN201220167162 U CN 201220167162U CN 202543220 U CN202543220 U CN 202543220U
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/04—Flat or tray type, drawers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M23/20—Material Coatings
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/06—Plates; Walls; Drawers; Multilayer plates
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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Abstract
The utility model discloses a high-throughput cell culture incubator which comprises a cell culture chamber. The cell culture chamber is formed by multiple independent cell culture layers partitioned by partitions, a culture medium feeding and discharging port and a sealing stopper are arranged in each cell culture layer, the sealing stoppers are inserted into ventilation ports with filters, each culture medium feeding and discharging port is connected with a feeding device through a pipeline, and each feeding device is connected with a culture solution storage tank and a cell collection bottle respectively through a pipeline. The high-throughput cell culture incubator can determine cell culture quantity according to needs, conveniently switch different culture media to meet requirements of different cell culture stages, conveniently adjust the cell culture quantity, can be used for research and development and actual production and is wider in application range.
Description
Technical field
The utility model relates to a kind of cell culture apparatus and preparation method thereof, particularly a kind of high-flux cell incubator and preparation method thereof.
Background technology
In recent ten years; Because the develop rapidly of biological products industry; Traditional pass through Measurement for Biochemistry and can not satisfy the demand in market far away from the method that animal tissues obtains biological products, expressing specific albumen, monoclonal antibody, Interferon, rabbit and virus vaccines goods through zooblast in external large scale culturing has become the most general technology at present.In addition, recent years, increasingly extensive to human body cell cultivation application, wherein, histoorgan reproduces with cell therapy became human disease treatment method the most likely along with the development of stem cells technology.Cell therapy is meant the somatocyte from body, allogeneic or xenogenesis (non-human body) that should choose, behind manipulation in vitro, feeds back the treat-ment of (or implantation) human body.This manipulation in vitro comprise cell external go down to posterity, increase, screening and medicine or other can change the processing of cytobiology behavior.Cell through behind the manipulation in vitro can be used for treatment of diseases, also can be used for the diagnosis or the prevention of disease.
The external large scale culturing technology of human body and zooblast is under artificial condition, sets pH, temperature, dissolved oxygen etc., the technology that is used to produce biological products in a large amount of culture of animal cells of cell culture vector (container) middle-high density.The zooblast of large scale culturing commonly used has multiple Yuan Dynasty cells such as CEF, former generation hamster kidney cell, and human diploid cell, Chinese hamster ovary celI, Vero cell etc.These cells are in product scope widespread uses such as production of vaccine, monoclonal antibody preparation, Hempoietine.Human body cell commonly used has stem cell, NK cell, skin cells etc.
Cell large scale cultural method commonly used at present has conventional cell cultures flask culture, rolling bottle cultivation, bioreactor culture and cell factory etc.The rolling bottle technology is traditional attached cell culture technique, and cell inoculation is in the round shape incubator-rolling bottle of rotation, and rolling bottle constantly rotates in the culturing process, makes cell turnover contact nutrient solution and air.The rolling bottle cultivation has simple in structure, less investment, and technology maturation is amplified advantages such as only needing simple increase rolling bottle quantity.But its shortcoming is also arranged: labour intensity is big, and it is big to take up an area of the space, and unit volume provides the surface-area of cell growth little, and the cell stand density is low, the difficult control etc. of bottle differences, thereby be difficult to industrialization or large-scale production.
By Corning company and the exploitation of Themo Fisher company, this device is pushed cell cultures to robotization, high efficiency, suitability for industrialized production, and great advance has been arranged " cell factory ".But this system fails to solve the problem that the cell non-invasi reclaims and polyclonal cellular is cultivated simultaneously of cultivating after finishing in a reactor drum.In addition, this system can't cultivate many batches or polyclonal cellular simultaneously in same cell culture apparatus;
Conventional Tissue Culture Dish, plate and bottle are open, and liquid feeding, inoculation and cell harvesting operating process are very easily polluted.
Be necessary to develop a kind of novel high-flux cell incubator.
The utility model content
The purpose of the utility model is to provide a kind of high-throughout cell culture apparatus.
The technical scheme that the utility model is taked is:
A kind of high-flux cell incubator; Comprise cell culture chamber; Cell culture chamber is isolated into a plurality of independently cell cultures layers by dividing plate to be formed, and is provided with substratum feed inlet and outlet and sealing plug in the cell cultures layer, is inserted with the vent port of band strainer in the sealing plug; The substratum feed inlet and outlet links to each other with feeding unit through pipeline, and feeding unit links to each other with the cell harvesting bottle with the nutrient solution storage tank respectively through pipeline.
The step of carrying out as the utility model improves, and feeding unit is single tube or multitube peristaltic pump.
The step of carrying out as the utility model improves, and nutrient solution storage tank top is provided with the vacuum type strainer.
The step of carrying out as the utility model improves, and cell culture chamber is made of plastics.
The step of carrying out as the utility model is improved the inwall of the inwall of cell cultures layer for handling through hydrophilic modifying.
The step of carrying out as the utility model improves, and sealing plug is a rubber seal plug.
The beneficial effect of the utility model is:
The cell culture apparatus of the utility model can be cultivated polyclonal cellular simultaneously, for the cells in vitro of clinical cell therapy is cultivated the ideal instrument that provides; Use cell culture apparatus of the present invention, cell cultures and reinforced and results thereof are carried out under closed state, significantly reduced its contaminated possibility in culturing process.
The cell culture apparatus of the utility model; Take the lead in developing the production equipment of the high precision that is used for cell cultures and cell and product results thereof, robotization, sequencing, continuous high-efficient at home; Cell cultures and cell and product thereof results operation is integrated in a reactor drum or a process step carries out; Thereby simplify technical process, enhance productivity, solve cell attachment and closed reinforced and cultivate the problems such as results after finishing;
The cell culture apparatus of the utility model; Can confirm the cultivation amount of cell as required, can switch different culture medium easily, satisfy the demand in different cell cultures stages; Can regulate the cultivation amount of cell easily; Both can be used for research and development, and also can be used for actual production, range of application is wider.
Handle through inwall being carried out modification, make the inwall possess hydrophilic property, thereby strengthened the adherent rate of cell, improved the culture efficiency of cell.
Description of drawings
Fig. 1 is the structural representation of the utility model cell culture apparatus;
Fig. 2 is the structural representation of the utility model cell culture chamber;
Fig. 3 is a kind of structural representation at the utility model sealing plug place;
Fig. 4 is a kind of structural representation of the utility model feeding unit.
Embodiment
Below in conjunction with accompanying drawing, carry out step explanation the utility model.
Referring to Fig. 1～4,
A kind of high-flux cell incubator; Comprise cell culture chamber 1; Cell culture chamber 1 is isolated into a plurality of independently cell cultures layers 2 by dividing plate to be formed, and is provided with substratum feed inlet and outlet 21 and sealing plug 22 in the cell cultures layer 2, is inserted with the vent port 24 of band strainer 23 behind the sealing plug 22; Substratum feed inlet and outlet 21 links to each other with feeding unit 3 through pipeline, and feeding unit 3 links to each other with cell harvesting bottle 5 with nutrient solution storage tank 4 respectively through pipeline.
The step of carrying out as the utility model improves, and feeding unit is single tube or multitube peristaltic pump.Peristaltic pump can be regulated flow velocity and flow easily, and can further reduce manual operation through the computer remote operation, has reduced the contaminated risk of cell.
The step of carrying out as the utility model improves, and nutrient solution storage tank 4 tops are provided with vacuum type strainer 41.The nutrient solution for preparing carries out degerming through the vacuum type strainer to be handled, and has made things convenient for the charging of nutrient solution.Also reduced simultaneously the contaminated risk of nutrient solution.
The step of carrying out as the utility model improves, and cell culture chamber is made of plastics.
The step of carrying out as the utility model is improved the inwall of the inwall of cell cultures layer for handling through hydrophilic modifying.Through the inwall of modification, can promote the adherent growth of cell better.
The step of carrying out as the utility model improves, and sealing plug is a rubber seal plug.
When culturing cell, when nutrient solution passes through the feeding unit charging, can carry out gaseous interchange through vent port, the air pressure with inside and outside the statocyte culture layer makes things convenient for charging.When inoculating cell, can syringe needle be inserted in the sealing plug, inoculating cell, inoculation is extracted after accomplishing, and sealing plug is airtight naturally, and is easy to operate, also is difficult for polluting nutrient solution.
Because the cell cultures layer is independently of one another, nutrient solution also can pass through the independent charging of feeding unit, therefore, can cultivate different cells in the different cells culture layer, also can get rid of waste liquid and new nutrient solution in the different steps of cell cultures.Use the cell culture apparatus of the utility model, can cultivate various kinds of cell easily, also can set the cultivation amount of cell as required.
After treating cell maturation, make cell take off wall, through feeding unit cultured extracellular is inhaled afterwards, be collected in the cell harvestor.
The cell culture apparatus of the utility model can be cultivated polyclonal cellular simultaneously, for the cells in vitro of clinical cell therapy is cultivated the ideal instrument that provides; Use cell culture apparatus of the present invention, cell cultures and reinforced and results thereof are carried out under closed state, significantly reduced its contaminated possibility in culturing process.
The cell culture apparatus of the utility model; Take the lead in developing the production equipment of the high precision that is used for cell cultures and cell and product results thereof, robotization, sequencing, continuous high-efficient at home; Cell cultures and cell and product thereof results operation is integrated in a reactor drum or a process step carries out; Thereby simplify technical process, enhance productivity, solve cell attachment and closed reinforced and cultivate the problems such as results after finishing;
The cell culture apparatus of the utility model; Can confirm the cultivation amount of cell as required, can switch different culture medium easily, satisfy the demand in different cell cultures stages; Can regulate the cultivation amount of cell easily; Both can be used for research and development, and also can be used for actual production, range of application is wider.
Claims (6)
1. high-flux cell incubator; Comprise cell culture chamber; It is characterized in that: said cell culture chamber is isolated into a plurality of independently cell cultures layers by plastic septum to be formed, and is provided with substratum feed inlet and outlet and sealing plug in the said cell cultures layer, is inserted with the vent port of band strainer in the said sealing plug; Said substratum feed inlet and outlet links to each other with feeding unit through pipeline, and said feeding unit links to each other with the cell harvesting bottle with the nutrient solution storage tank respectively through pipeline.
2. a kind of high-flux cell incubator according to claim 1 is characterized in that: feeding unit is single tube or multitube peristaltic pump.
3. according to claim 1 or 2 described a kind of high-flux cell incubators, it is characterized in that: nutrient solution storage tank top is provided with the vacuum type strainer.
4. a kind of high-flux cell incubator according to claim 1, it is characterized in that: cell culture chamber is made of plastics.
5. a kind of high-flux cell incubator according to claim 1 is characterized in that: sealing plug is a rubber seal plug.
6. a kind of high-flux cell incubator according to claim 4 is characterized in that: the inwall of the inwall of cell cultures layer for handling through hydrophilic modifying.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220167162 CN202543220U (en) | 2012-04-18 | 2012-04-18 | High-throughput cell culture incubator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220167162 CN202543220U (en) | 2012-04-18 | 2012-04-18 | High-throughput cell culture incubator |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202543220U true CN202543220U (en) | 2012-11-21 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201220167162 Expired - Lifetime CN202543220U (en) | 2012-04-18 | 2012-04-18 | High-throughput cell culture incubator |
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| Country | Link |
|---|---|
| CN (1) | CN202543220U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013155962A1 (en) * | 2012-04-18 | 2013-10-24 | 广州洁特生物过滤制品有限公司 | High throughput cell incubator and manufacturing method thereof |
| CN111748468A (en) * | 2019-03-28 | 2020-10-09 | 上海尚瑞生物医药科技有限公司 | A multi-layered petri dish |
-
2012
- 2012-04-18 CN CN 201220167162 patent/CN202543220U/en not_active Expired - Lifetime
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013155962A1 (en) * | 2012-04-18 | 2013-10-24 | 广州洁特生物过滤制品有限公司 | High throughput cell incubator and manufacturing method thereof |
| CN111748468A (en) * | 2019-03-28 | 2020-10-09 | 上海尚瑞生物医药科技有限公司 | A multi-layered petri dish |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C56 | Change in the name or address of the patentee |
Owner name: GUANGZHOU JET BIOLOGICAL FILTRATION CO., LTD. Free format text: FORMER NAME: GUANGZHOU JET BIOLOGICAL FILTRATION PRODUCTS CO., LTD. |
|
| CP03 | Change of name, title or address |
Address after: 511356, No. 1, beat Tong Road, Yonghe Economic Zone, Guangzhou economic and Technological Development Zone, Guangdong Patentee after: Guangzhou Jie Te biofiltration limited-liability company Address before: 510730 Friendship Road 173, Guangzhou economic and Technological Development Zone, Guangdong, China Patentee before: Guangzhou Jet Bio-Filtration Products Co., Ltd. |
|
| CX01 | Expiry of patent term |
Granted publication date: 20121121 |
|
| CX01 | Expiry of patent term |