CN202057586U - Chlorophyll fluorescence spectrum analyzing device - Google Patents
Chlorophyll fluorescence spectrum analyzing device Download PDFInfo
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- CN202057586U CN202057586U CN2010206563591U CN201020656359U CN202057586U CN 202057586 U CN202057586 U CN 202057586U CN 2010206563591 U CN2010206563591 U CN 2010206563591U CN 201020656359 U CN201020656359 U CN 201020656359U CN 202057586 U CN202057586 U CN 202057586U
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- chlorophyll
- light source
- chlorophyll fluorescence
- spectral analysis
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Abstract
The utility model relates to a chlorophyll fluorescence spectrum analyzing device, which comprises a temperature sensor and an external light source sensor, wherein the temperature sensor and the external light source sensor are electrically connected.
Description
[technical field]
The utility model relates to a kind of chlorophyll fluorescence spectral analysis device.
[background technology]
Photosynthesis provides the basis of metabolism of plant all substances and energetic supersession, it comprises the complex process that a series of optical physicss, photochemistry and biological chemistry change, at photosynthetic primitive reaction, to absorb the luminous energy transmission, be converted in the process of electric energy, some luminous energy loss discharges (usually less than 1% projectile energy) with long fluorescence mode.Research and survey that photosynthetic fluorescent characteristic has crucial scientific meaning and using value under this natural conditions.At first, chlorophyll fluorescence under the natural conditions and photosynthesis have very confidential relation.On the one hand, when plant was exposed under the strong illumination condition, fluorescence is being played the part of crucial protective effect, avoids chloroplast to absorb luminous energy and surpasses photosynthetic digestion power, and the loss that high light is burnt is reduced to minimum; On the other hand, in general, chlorophyll fluorescence and photosynthetic rate are to be mutually related under the natural conditions, and photosynthetic rate is higher, then fluorescence a little less than, vice versa.So, can understand photosynthesis of plants indirectly by surveying chlorophyll fluorescence.Secondly, as " probe " of plant health situation, the photosynthesis fluorescent characteristic is relevant with the degree of being coerced with the nutrition of plant under the natural conditions.Therefore, can understand physiology, generation, the disease of plant and be threatened by force state by the fluorescent characteristic detection of photosynthesis of plant.
Chlorophyll is the green pigment in the photosynthetic membrane, and it is a capturing optical principal ingredient in the photosynthesis.It is several that chlorophyll has a, b, c, d and e etc.All plants that discharges oxygen when carrying out photosynthesis all contain chlorophyll a; Chlorophyll b is present in higher plant, green alga and the euglena; Chlorofucsin is present in diatom, flagellate and the brown alga, and CHLOROPHYLL d is present in red algae.Chlorophyll in the higher plant chloroplast mainly contains two kinds of chlorophyll a and chlorophyll bs.The molecular structure of chlorophyll a is connected to form ring texture by 4 pyrrole rings by 4 methenes, is called porphyrin.Porphyrin ring is central in conjunction with 1 magnesium atom, and a cyclopentanone (V) is arranged, and it is water-soluble to form the sylvite tool after the propionic acid on the ring IV is by phytol esterification, saponification.They are water insoluble, and are dissolved in organic solvent, as ethanol, acetone, ether, chloroform etc.
The variation of nearly all photosynthesis process all can reflect by chlorophyll fluorescence, and the fluorescence detection technology does not need smudge cells, do not injure biosome, and chlorophyllous quantum yield and chlorophyllous concentration are linear, therefore can utilize the chlorophyll fluorescence spectral analysis device to measure chlorophyllous quantum yield, and then measure chlorophyll concentration.
[utility model content]
The purpose of this utility model provides a kind of chlorophyll fluorescence spectral analysis device and measures the method for chlorophyll concentration.
The utility model embodiment provides a kind of chlorophyll fluorescence spectral analysis device, and this chlorophyll fluorescence spectral analysis device comprises temperature sensor and external light source sensor, and this temperature sensor electrically connects with this external light source sensor.
Preferably, this chlorophyll fluorescence spectral analysis device detects the electron transport speed in the plant.
Preferably, this chlorophyll fluorescence spectral analysis device further comprises the light source detection device, and this light source detection device electrically connects with this external light source sensor.
Preferably, this light source detection device and this temperature sensor electrically connect.
[description of drawings]
Comprise accompanying drawing providing, and accompanying drawing is incorporated in this instructions and is constituted the some of this instructions into for further understanding of the present utility model.The one exemplary embodiment of description of drawings the utility model.At Zhu Tuzhong:
Fig. 1 is the structural representation of the utility model chlorophyll fluorescence spectral analysis device preferred embodiment;
Fig. 2 is dried barrier film I experimental result data table;
Fig. 3 is dried barrier film II experimental result data table;
Fig. 4 is wet barrier film I experimental result data table; And
Fig. 5 is wet barrier film II experimental result data table.
[embodiment]
Below in conjunction with drawings and Examples the utility model embodiment is elaborated.
Chlorophyllous quantum yield and chlorophyllous concentration are linear, thus can utilize the chlorophyll fluorescence spectral analysis device to measure the chlorophyllous quantum yield of extraction, and then the calibrating chlorophyll concentration.The quantum yield scope of general high terrestrial plant is about 0.01～0.99, and the chlorophyll quantum yield scope of extraction is about about 0.07～0.5 at present, and in the utility model embodiment, concrete steps are as described below.
Fig. 1 is the structural representation of the utility model chlorophyll fluorescence spectral analysis device preferred embodiment.As shown in Figure 1, the utility model embodiment provides a kind of chlorophyll fluorescence spectral analysis device 10, this chlorophyll fluorescence spectral analysis device 10 comprises test side (figure does not show), this test side comprises light source detection device (figure does not show), temperature sensor 12 and external light source sensor 14, the electron transport speed that this chlorophyll fluorescence spectral analysis device 10 detects in the plant.
This light source detection device is arranged on this temperature sensor 12 tops, does not contact setting with this temperature sensor 12, and this detection light source device electrically connects with this temperature sensor 12, this external light source sensor 14 respectively.
This temperature sensor 12 contacts setting with this external light source sensor 14, and this temperature sensor 12 electrically connects with this external light source sensor 14.
The utility model also provides a kind of method of measuring chlorophyll concentration, can measure chlorophyllous quantum yield by above-mentioned chlorophyll fluorescence spectral analysis device, and then measure chlorophyllous concentration.
In the present embodiment, the sample of chlorophyll fluorescence spectral analysis device detection is divided into four kinds: dried barrier film I, dried barrier film II, wet barrier film I and wet barrier film II.
Fig. 2 is dried barrier film I experimental result data table.This dried barrier film I adheres to the combination salt compounds, and as shown in Figure 2, the experimental result of detection is as follows: F: minimum fluorescent value is 27; Fm ': the maximum fluorescence value is 37; PAR: effectively fluorescent value is 0.By the experimental result data table as can be seen, because this dried barrier film I only adheres to the combination salt compounds, so the experimental result of this dried barrier film I does not have the data presentation of chlorophyll quantum yield and chlorophyll electron transport speed.
Fig. 3 is dried barrier film II experimental result data table.This dried barrier film II adheres to combination salt compounds and chlorophyll, and as shown in Figure 3, the experimental result of detection is: F: minimum fluorescent value is 59, Fm ': the maximum fluorescence value is 63, PAR: effectively fluorescent value is 0, and Y (II): photosystem II quantum yield is 0.063, ETR: electron transport speed is 0.
Fig. 4 is wet barrier film I experimental result data table.Be somebody's turn to do wet barrier film I attached water, combination salt compounds and chlorophyll, as shown in Figure 3, the experimental result of detection is: F: minimum fluorescent value is 86; Fm ': the maximum fluorescence value is 115; PAR: effectively fluorescent value is 0; Y (II): photosystem II quantum yield is 0.252; ETR: electron transport speed is 0.
Fig. 5 is wet barrier film II experimental result data table.Should adhere to alcohol, combination salt compounds and chlorophyll by wet barrier film II, chlorophyll is high concentration chlorophyll, and as shown in Figure 3, the experimental result of detection is: F: minimum fluorescent value is 78; Fm ': the maximum fluorescence value is 161; PAR: effectively fluorescent value is 0; Y (II): photosystem II quantum yield is 0.516; ETR: electron transport speed is 0.
In embodiment of the present utility model, by above-mentioned experimental result as can be known, under mixed solvent (promptly wet barrier film II adheres under alcohol, combination salt compounds and the high concentration chlorophyll situation), chlorophyll can reach best quantum yield, photosystem II quantum yield is 0.516, measures dried barrier film I, II and the chlorophyll fluorescence effect can not take place under dry situation.
Only be preferred case study on implementation of the present utility model below, be not limited to the utility model, for a person skilled in the art, the utility model can have various changes and variation.All within spirit of the present utility model and principle, any modification of being done, be equal to replacement, improvement etc., all should be included within the protection domain of the present utility model.
Claims (3)
1. chlorophyll fluorescence spectral analysis device, it is characterized in that: this chlorophyll fluorescence spectral analysis device comprises temperature sensor and external light source sensor, this temperature sensor with should external light source sensor electric connection.
2. chlorophyll fluorescence spectral analysis device according to claim 1 is characterized in that: this chlorophyll fluorescence spectral analysis device further comprises the light source detection device, and this light source detection device electrically connects with this external light source sensor.
3. chlorophyll fluorescence spectral analysis device according to claim 2 is characterized in that: this light source detection device and this temperature sensor electrically connect.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010206563591U CN202057586U (en) | 2010-12-13 | 2010-12-13 | Chlorophyll fluorescence spectrum analyzing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010206563591U CN202057586U (en) | 2010-12-13 | 2010-12-13 | Chlorophyll fluorescence spectrum analyzing device |
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| CN202057586U true CN202057586U (en) | 2011-11-30 |
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| CN2010206563591U Expired - Fee Related CN202057586U (en) | 2010-12-13 | 2010-12-13 | Chlorophyll fluorescence spectrum analyzing device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102564967A (en) * | 2010-12-13 | 2012-07-11 | 依诺特生物能量控股公司 | Chlorophyll fluorescent spectroscopy analyzer and method for measuring chlorophyll concentration |
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- 2010-12-13 CN CN2010206563591U patent/CN202057586U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102564967A (en) * | 2010-12-13 | 2012-07-11 | 依诺特生物能量控股公司 | Chlorophyll fluorescent spectroscopy analyzer and method for measuring chlorophyll concentration |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20111130 Termination date: 20191213 |