CN201852839U - Immunofluorescence analyzer - Google Patents
Immunofluorescence analyzer Download PDFInfo
- Publication number
- CN201852839U CN201852839U CN201020575269XU CN201020575269U CN201852839U CN 201852839 U CN201852839 U CN 201852839U CN 201020575269X U CN201020575269X U CN 201020575269XU CN 201020575269 U CN201020575269 U CN 201020575269U CN 201852839 U CN201852839 U CN 201852839U
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- operational amplifier
- ground end
- integrating amplifier
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- Expired - Lifetime
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- 238000005259 measurement Methods 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
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- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
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- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- FDWREHZXQUYJFJ-UHFFFAOYSA-M gold monochloride Chemical compound [Cl-].[Au+] FDWREHZXQUYJFJ-UHFFFAOYSA-M 0.000 description 1
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- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
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- LRBQNJMCXXYXIU-NRMVVENXSA-N tannic acid Chemical compound OC1=C(O)C(O)=CC(C(=O)OC=2C(=C(O)C=C(C=2)C(=O)OC[C@@H]2[C@H]([C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)O2)OC(=O)C=2C=C(OC(=O)C=3C=C(O)C(O)=C(O)C=3)C(O)=C(O)C=2)O)=C1 LRBQNJMCXXYXIU-NRMVVENXSA-N 0.000 description 1
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- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The utility model discloses an immunofluorescence analyzer and belongs to the technical field of test instruments. The immunofluorescence analyzer is used for solving problems including low signal to noise ratio, low sensitivity, low test speed, complex operation, high test cost and high request for detection environment of the existing immunofluorescence analyzer when being used for detecting hormone or pesticide residue of food. The immunofluorescence analyzer comprises a microprocessor, a displayer, a key, an IC card, a printer, an indicator light, a power source, a laser (102) and a sensor, wherein a pin 1 of the microprocessor is connected with the power source; the power source supplies the working voltage Vcc to all parts; a pin 2 of the microprocessor is connected with the sensor; a pin 8 of the microprocessor is connected with the laser; a pin 3 of the microprocessor is connected with the displayer; a pin 4 of the microprocessor is connected with the key; a pin 5 of the microprocessor is connected with the IC card; a pin 6 of the microprocessor is connected with the printer; and a pin 7 of the microprocessor is connected with the indicator light. The immunofluorescence analyzer can be widely used for detecting hormone or pesticide residue of food.
Description
Technical field
The utility model relates to laser dye and the application of multiple spot calibration technology in the chromatography quantitative test in a kind of clinical diagnosis field and the food security monitoring field.Be specially adapted to the clinical diagnosis field and do immune quantitative analysis and food service industry, liquor industry, beverage industry, dairy industry, occasions such as cosmetic industry are made the immunofluorescence analysis instrument of hormone and residues of pesticides in the fast detecting sample to be checked.
Background technology
Immunochromatography technique is a kind of novel immunolabelling technique that is applied to antigen-antibody with chromatography as the tracer label thing.Chromatography is by gold chloride (HAuCl
4) under effects such as reductive agent such as white phosphorus, ascorbic acid, sodium citrate, tannic acid, polymerization becomes the gold grain of specific size, and, be called chromatography because electrostatic interaction becomes a kind of stable colloidal state.Chromatography is electronegative under the weak base environment, can form firm combining with the positive charge group of protein molecule, because this combination is the static combination, so do not influence the biological nature of protein.
Chromatography except with protein bound, can also combine with many other biomacromolecules, as SPA, PHA, ConA etc.According to some physical behaviors of chromatography, as high electron density, grain size, shape and color reaction, add the immunity and the biological characteristics of bond, thereby make chromatography be widely used in fields such as immunology, histology, pathology and cell biology.
Clinical diagnosis (IVD) industry has recognized that the chromatography immunoassay all is a good technology platform concerning POC and OTC market, because its cost is low, it is also convenient to use.
Most of commercial clinical diagnosis (IVD) products use immune platform that qualitative and semiquantitative result can only be provided.Promptly judge that by the T line (p-wire) on the range estimation test card and the relativeness of C line (nature controlling line) testing sample is negative or positive: such as, the colour developing of C line is working properly for reagent card, and the colour developing of T line is positive, and the T line does not develop the color negative.Though some emerging chromatography detection techniques that quantitative measurment is provided have been developed or developed to the IVD industry, but methods: promptly make the positive, negative judgement by the depth of p-wire color owing to the identification of whole employing color, or estimate the concentration of sample to be tested substantially, therefore detect all gratifying analytical equipment aspect performance and the cost without any technology at conventional chromatography at present.
Summary of the invention
The utility model overcomes deficiency of the prior art, provide a kind of high s/n ratio, high sensitivity, high test speed, easy and simple to handle, testing cost is cheap, testing environment requires low immunofluorescence analysis instrument.
In order to solve the problem of above-mentioned technology, the utility model is achieved through the following technical solutions: the immunofluorescence analysis instrument comprises microprocessor, display, button, IC-card, printer, pilot lamp and power supply, also comprise laser instrument and sensor, microprocessor 1 pin is connected with power supply, power supply provides operating voltage Vcc to each parts, microprocessor 2 pin are connected with sensor, microprocessor 8 pin are connected with laser instrument, microprocessor 3 pin are connected with display, microprocessor 4 pin are connected with button, microprocessor 5 pin are connected with IC-card, and microprocessor 6 pin are connected with printer, and microprocessor 7 pin are connected with pilot lamp.
The utility model also can be achieved through the following technical solutions: above-described laser instrument comprises triode, operational amplifier, microprocessor and laser tube, operational amplifier 1 pin is connected with the base stage of triode, the collector of triode is connected with laser tube 2 pin, laser tube 1 pin goes out to hold the Vcc voltage end to be connected by resistance R 3 and power supply, laser tube 1 pin is by capacitor C 4 back earth terminals, operational amplifier 2 pin are connected with the emitter of triode, operational amplifier 2 pin are by resistance R 5 earth terminals, earth terminal behind the operational amplifier 3 pin series resistor R2, be connected with microprocessor 13 pin behind the operational amplifier 3 pin series resistor R1, microprocessor 11 pin serial connection capacitor C 1 back is connected with microprocessor 14 pin, microprocessor 12 pin are connected with operational amplifier 7 pin, microprocessor 12 pin are connected with operational amplifier 6 pin with resistance R 6 by capacitor C 2 respectively, capacitor C 2, resistance R 6 is connected with laser tube 4 pin with operational amplifier 6 pin, laser tube 3 pin are respectively by capacitor C 3 and resistance R 7 earth terminals, laser tube 3 pin, capacitor C 3 is connected with resistance R 4 with operational amplifier 5 pin respectively with resistance R 7, resistance R 4 goes out to hold the Vcc voltage end to be connected with power supply, operational amplifier 4 pin earth terminals.
The utility model can also be achieved through the following technical solutions: above-described sensor comprises photoelectric sensor, integrating amplifier, duodiode and microprocessor, integrating amplifier 1 pin is connected with the ground end, integrating amplifier 3 pin, 4 pin, 5 pin are connected with photoelectric sensor 2 ends with 6 pin, photoelectric sensor 1 end is connected with the ground end, integrating amplifier 7 pin are connected with the ground end, integrating amplifier 8 pin are connected with the ground end and are connected with simulation ground end by capacitor C 3, integrating amplifier 14 pin are connected with simulation ground end, integrating amplifier 10 pin serial connection duodiode 1 pin connects, duodiode 3 pin are connected with the ground end, integrating amplifier 10 pin serial connection capacitor C 5 is connected with the ground end, integrating amplifier 10 pin series resistor R1 are connected with microprocessor 2 pin, integrating amplifier 9 pin are connected with the ground end by capacitor C 4, integrating amplifier 9 pin are connected with simulation ground end, integrating amplifier 13 pin earth terminals, integrating amplifier 12 pin are connected with microprocessor 15 pin, and integrating amplifier 11 pin are connected with simulation ground end.
Compared with prior art, the beneficial effects of the utility model are:
1. the utility model has adopted laser instrument 102, sensor 103 to realize utilizing the laser dye principle effectively to improve the signal to noise ratio (S/N ratio) of system, and by two nature controlling line real time calibrations of C1, C2 curve that dispatches from the factory, signal to noise ratio (S/N ratio) is effectively controlled in test by B point ground unrest, and the relativeness by T line and C1, C2 line is as the concentration of variable by the curve calculation sample to be tested that dispatches from the factory after proofreading and correct.Therefore be the alternative method of conventional chromatography range estimation or colorimetric detection, have high s/n ratio, high sensitivity, high test speed, the multi-parameter fitting mathematical model of microprocessor has improved instrument test sensitivity, and its sensitivity and test speed obviously are better than common method of testing.
2. the utility model adopts broadband integrating amplifier 202,302, adopt technology such as microprocessor 101 to improve automatic calibration, automatic data processing, degree such as judgement as a result automatically simultaneously, shortened analysis process, simplified staff's running program, made product realize quick, convenient, performance accurately.
3. the utility model is rational in infrastructure, superior performance, testing cost is cheap, testing environment requires low, do not need in the laboratory, can detect sample easily outdoor, particularly suitable is very practical in the production health and the environment fast monitored of industries such as dairy produce, water source, medicine, chemical industry, papermaking, cosmetics.
Description of drawings
Fig. 1 is a functional-block diagram of the present utility model.
Fig. 2 is the electrical schematic diagram of laser instrument 102 of the present utility model.
Fig. 3 is the electrical schematic diagram of sensor 103 of the present utility model.
Embodiment
Below in conjunction with the drawings and specific embodiments the utility model is described in further detail: the immunofluorescence analysis instrument comprises microprocessor 101, laser instrument (102), sensor (103) display 104, button 105, IC-card 106, printer 107, pilot lamp 108 and power supply 109 compositions.Fig. 1 is a functional-block diagram of the present utility model.The effect of microprocessor 101 is that result is handled and exported to the input data, and embodiment adopts monolithic P89V51RD2 type microprogramming device to make.The effect of display 104 is to show measurement result, and embodiment adopts LCD1604 escope spare to make.The effect of button 105 is input operation signals, and embodiment adopts the customized adhesive cover button to make.The effect of IC-card 106 is the data of reading in the attached card of reagent, and embodiment adopts KF-013M type IC-card to make.The effect of printer 107 is printout measurement results, and embodiment adopts ELM205 type printer to make.The effect of pilot lamp 108 is duty indications, and embodiment adopts 3mmled type pilot lamp to make.The effect of power supply 109 provides each parts operating voltage, and its output Vcc voltage is+5V that embodiment adopts commercially available 17805 type integrated power supplies to make.
The effect of the utility model laser instrument 102 is as stabilized light source, it is made up of triode 201, operational amplifier 202, laser tube 204 and microprocessor 101, as shown in Figure 2, Fig. 2 is the electrical schematic diagram of laser instrument 102 of the present utility model, and embodiment presses Fig. 2 connection line.Wherein the effect of triode 201 is the higher level to be sent here electric signal carry out electric current amplification rear drive laser tube, adopts S9013 type triode to make.Wherein the effect of operational amplifier 202 is that the electric current current stabilization control of triode 201 and the feedback signal of laser tube inside are amplified, and adopts TLC2272 type operational amplifier to make.Wherein the effect of laser tube 204 is to send beam of laser as light source and the brightness monitoring diode is arranged, and adopts LD660 type laser tube to make.Luminance signal input microprocessor 101 input brightness control signals among the embodiment, after processing of circuit, export laser tube 204 afterwards to, laser tube 204LD part is luminous under the control of electric signal, in the laser tube 204 inner PD brightness of monitoring laser simultaneously, and send the luminance signal that monitors back to operational amplifier, the signal after amplifying finally feeds back to microprocessor 101 to be handled.
The effect of the utility model sensor 103 is to receive faint fluorescence signal and processing and amplifying.It is made up of photoelectric sensor 301, integrating amplifier 302, duodiode 303 and microprocessor (101), as shown in Figure 3, presses Fig. 3 connection line.Wherein the effect of photoelectric sensor 301 is to gather fluorescence signal.Embodiment adopts commercially available S2371 type photoelectric sensor to make.The effect of integrating amplifier 302 is that the feeble signal of photoelectric sensor 301 is carried out processing and amplifying.Embodiment adopts commercially available IVC102 type integrating amplifier to make.The effect of duodiode 303 is the negative pulses of integrating amplifier output of releasing.Embodiment adopts commercially available M1MA142WATIG type duodiode to make.
All resistance, the capacitance component that the utility model adopts all adopts commercially available general resistance, capacitor element to make.
The concise and to the point principle of work of the utility model is as follows: after inserting measured object, laser instrument 102 sends the laser radiation measured object under the control of microprocessor 101, measured object sends fluorescence under the exciting of laser, this fluorescence is accepted by sensor 103 parts and is treated to electric signal, and 101 processing obtain and the closely-related numerical value of measured object content thereby this electric signal is issued microprocessor.Be ready to reagent card, the sample drop three of need tests is dripped to the sample well of reagent card, the tray member that balance after 3 minutes is put into reagent card instrument detects the cabin, starts the test button and measures.At this moment sensor 103 opticators at first start laser tube 204 and make it luminous, the PD of detection laser diode holds the pilot signal that returns simultaneously, after microprocessor 101 computings, carry out self calibration in the brightness of control laser, light source begins to scan reagent card then, microprocessor 101 writes down C1 line, C2 line and T line and B location context signal successively, the spray film is good in advance on this card by chemical reagent work for each bar line on the test card, promptly generates discernible lines after adding sample after the position of every line sample and spray film bag are reacted.Fluorescence intensity is measured under the scanning of laser tube 204 through the reacted reagent card of application of sample; and use microprocessor 101 the dispatch from the factory curve built-in according to the test result correction instrument of C1 line and C2 line; microprocessor 101 is by B location context signal testing result treatment ground unrest simultaneously; microprocessor 101 is according to T line and C1 line; the relative data relation of C2 line is brought the revised curve that dispatches from the factory into; microprocessor 101 calculates the concentration of testing sample; whole test process is operated control by microprocessor; and by display 104 demonstrations and keyboard 105 settings; realize the Automatic Control of test process; test result is finished whole test process by the data-carrier store protection or by built-in printer 107 outputs.
The utility model mounting structure is as follows:
It is in 280 * 210 * 130 millimeters tool housings that the utility model accompanying drawing 1 and all circuit blocks are installed in a long * wide * height, display 104, printer 107, button 105 are installed on the front panel of tool housing, the interface of data transmission and outer computer is housed, the assembly cost utility model in the side of shell.
Claims (3)
1. immunofluorescence analysis instrument, it comprises microprocessor (101), display (104), button (105), IC-card (106), printer (107), pilot lamp (108) and power supply (109), it is characterized in that also comprising laser instrument (102) and sensor (103), microprocessor (101) 1 pin are connected with power supply (109), power supply (109) provides operating voltage Vcc to each parts, microprocessor (101) 2 pin are connected with sensor (103), microprocessor (101) 8 pin are connected with laser instrument (102), microprocessor (101) 3 pin are connected with display (104), microprocessor (101) 4 pin are connected with button (105), microprocessor (101) 5 pin are connected with IC-card (106), microprocessor (101) 6 pin are connected with printer (107), and microprocessor (101) 7 pin are connected with pilot lamp (108).
2. immunofluorescence analysis instrument as claimed in claim 1, it is characterized in that laser instrument (102) comprises triode (201), operational amplifier (202), microprocessor (101) and laser tube (204), operational amplifier (202) 1 pin are connected with the base stage of triode (201), the collector of triode (201) is connected with laser tube (204) 2 pin, laser tube (204) 1 pin go out to hold the Vcc voltage end to be connected by resistance R 3 with power supply (109), laser tube (204) 1 pin are by capacitor C 4 back earth terminals, operational amplifier (202) 2 pin are connected with the emitter of triode (201), operational amplifier (202) 2 pin are by resistance R 5 earth terminals, earth terminal behind operational amplifier (202) the 3 pin series resistor R2, be connected with microprocessor (101) 13 pin behind operational amplifier (202) the 3 pin series resistor R1, microprocessor (101) 11 pin serial connection capacitor C 1 back is connected with microprocessor (101) 14 pin, microprocessor (101) 12 pin are connected with operational amplifier (202) 7 pin, microprocessor (101) 12 pin are connected with operational amplifier (202) 6 pin with resistance R 6 by capacitor C 2 respectively, capacitor C 2, resistance R 6 is connected with laser tube (204) 4 pin with operational amplifier (202) 6 pin, laser tube (204) 3 pin are respectively by capacitor C 3 and resistance R 7 earth terminals, laser tube (204) 3 pin, capacitor C 3 is connected with resistance R 4 with operational amplifier (202) 5 pin respectively with resistance R 7, resistance R 4 goes out to hold the Vcc voltage end to be connected with power supply (109), operational amplifier (202) 4 pin earth terminals.
3. immunofluorescence analysis instrument as claimed in claim 1 or 2, it is characterized in that sensor (103) comprises photoelectric sensor (301), integrating amplifier (302), duodiode (303) and microprocessor (101), integrating amplifier (302) 1 pin are connected with the ground end, integrating amplifier (302) 3 pin, 4 pin, 5 pin are connected with photoelectric sensor (301) 2 ends with 6 pin, photoelectric sensor (301) 1 ends are connected with the ground end, integrating amplifier (302) 7 pin are connected with the ground end, integrating amplifier (302) 8 pin are connected with the ground end and are connected with simulation ground end by capacitor C 3, integrating amplifier (302) 14 pin are connected with simulation ground end, integrating amplifier (302) 10 pin serial connection duodiode (303) 1 pin connect, duodiode (303) 3 pin are connected with the ground end, integrating amplifier (302) 10 pin serial connection capacitor C 5 is connected with the ground end, integrating amplifier (302) 10 pin series resistor R1 are connected with microprocessor (101) 2 pin, integrating amplifier (302) 9 pin are connected with the ground end by capacitor C 4, integrating amplifier (302) 9 pin are connected with simulation ground end, integrating amplifier (302) 13 pin earth terminals, integrating amplifier (302) 12 pin are connected with microprocessor (101) 15 pin, and integrating amplifier (302) 11 pin are connected with simulation ground end.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201020575269XU CN201852839U (en) | 2010-10-26 | 2010-10-26 | Immunofluorescence analyzer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201020575269XU CN201852839U (en) | 2010-10-26 | 2010-10-26 | Immunofluorescence analyzer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201852839U true CN201852839U (en) | 2011-06-01 |
Family
ID=44095227
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201020575269XU Expired - Lifetime CN201852839U (en) | 2010-10-26 | 2010-10-26 | Immunofluorescence analyzer |
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| Country | Link |
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| CN (1) | CN201852839U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109211865A (en) * | 2018-11-17 | 2019-01-15 | 郑州亲和力科技有限公司 | A kind of quantitative fluorescence analysis instrument |
-
2010
- 2010-10-26 CN CN201020575269XU patent/CN201852839U/en not_active Expired - Lifetime
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109211865A (en) * | 2018-11-17 | 2019-01-15 | 郑州亲和力科技有限公司 | A kind of quantitative fluorescence analysis instrument |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Assignee: SHIJIAZHUANG HIPRO BIOTECHNOLOGY CO., LTD. Assignor: Hao Shushun Contract record no.: 2013130000046 Denomination of utility model: Immunofluorescence analyzer Granted publication date: 20110601 License type: Exclusive License Record date: 20130613 |
|
| LICC | Enforcement, change and cancellation of record of contracts on the licence for exploitation of a patent or utility model | ||
| ASS | Succession or assignment of patent right |
Owner name: SHIJIAZHUANG HEBAI BIOTECHNOLOGY CO., LTD. Free format text: FORMER OWNER: HAO SHUSHUN Effective date: 20130905 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20130905 Address after: 050091 Hebei province Shijiazhuang City Xinshi North Road, No. 368 building, room 508, innovation Patentee after: SHIJIAZHUANG HIPRO BIOTECHNOLOGY CO., LTD. Address before: 050091 Hebei province Shijiazhuang City Xinshi North Road, No. 368 Jinshi Industrial Park Innovation Building Room 508 Patentee before: Hao Shushun |
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| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20110601 |