CN201433218Y - Portable CCD imaging detecting device for luminescent bacterium - Google Patents
Portable CCD imaging detecting device for luminescent bacterium Download PDFInfo
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- CN201433218Y CN201433218Y CN2009200145927U CN200920014592U CN201433218Y CN 201433218 Y CN201433218 Y CN 201433218Y CN 2009200145927 U CN2009200145927 U CN 2009200145927U CN 200920014592 U CN200920014592 U CN 200920014592U CN 201433218 Y CN201433218 Y CN 201433218Y
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The utility model relates to a portable CCD imaging detecting device for luminescent bacterium, which comprises a camera obscura, a microwell plate for holding luminescent bacterium, a reaction tank,a CCD digital camera and a computer. The CCD digital camera is arranged at the lowermost part in the camera obscura 1; the microwell plate for holding luminescent bacterium is on top of a medial axisof the CCD digital camera; the CCD digital camera is connected with the computer via a data wire; and an image processing software is arranged in the computer. When the luminescent bacterium sample liquid is added in the reaction tank, the fluorescence change of the bacterium is captured by the CCD digital camera, and is converted into the digital signal after inputting in the computer via the data wire, then the digital signal is analyzed by the software in the computer. The utility model has the advantages of portability, fast detection, high throughput and simple operation so as to meet therequirement of fast detection of a great amount of samples in the field of the sudden marine pollution disaster; and lays the foundation for the portable seawater comprehensive toxicity fast detecting device on a basis of the bacteria luminescent imaging technology.
Description
Technical field
The utility model relates to a kind of portable CCD imaging photogenic bacterium proofing unit.
Background technology
The luminescence process of bacterium is a kind of metabolic physiological process in the thalline, is the photorespiration process, and this luminescence process very easily is subjected to the influence of ambient conditions.Any factor of interference or infringement bacterial respiratory or physiological process all makes bacterial luminescence intensity change.Utilize the principle of luminosity of photogenic bacterium can reflect the comprehensive toxicity situation of water quality at short notice, simultaneously detected result is carried out assay, judge whether safety of water body, improve the accuracy of emergency monitoring, shorten the reaction times of emergency monitoring, thereby can increase substantially the speed and the effect of emergent decision-making and emergency disposal.
Change of luminous intensity can be noted on the tester of being made up of photomultiplier transit and amplifier.At present, the Microtox detection system of the U.S. and Nanjing soil institute of Chinese Academy of Sciences noclilucence photometer etc. belongs to desk-top instrument, though can be used for large batch of sample detection analysis, carries inconvenience, and instrument is applicable to that the laboratory internal fixing detects.The Deletox portable detection system of the U.S. is light small and exquisite, easy to carry, and the scene that is mainly used in water sample is detected, but once can only detect a sample, the only detection of suitable small amount of sample.
The utility model content
The purpose of this utility model is to utilize charge coupled device (Charge Coupled Device, the CCD) advantage of imaging analysis technology provide a kind of and can realize field quick detection, detect the portable photogenic bacterium proofing unit of a plurality of samples simultaneously.
(Charge Coupled Device, CCD) the imaging analysis technology is emerging in recent years a kind of new and high technology to charge coupled device.Thereby it is to utilize computer to analyze the purpose that ccd image reaches test, promptly utilize the powerful data operation ability of ccd sensor and computer that optical imagery is measured automatically, handled and analyzes, can obtain the Various types of data of analyte, and provide result data, the classification results of test or show in real time etc.It has advantages such as volume is little, highly sensitive, shock resistance is good, be not subjected to the disturbance of electromagnetic field, and simple to operate, sample flux is big.
Principle of the present utility model is: the fluorescence that photogenic bacterium sends can be responded to by the CCD digital camera, and when digital camera is taken pictures to photogenic bacterium, the variation of photogenic bacterium luminous intensity will go on record.Use data line that digital camera is connected with computer, by computer optical signal is transformed into electrical signal, appliance computer is handled data again.
The technical solution of the utility model is: a kind of portable CCD imaging photogenic bacterium proofing unit, form by camera bellows 1, photogenic bacterium microwell plate 2, reaction tank 3, CCD digital camera 4 and computer 5.CCD digital camera 4 is located at the below in the camera bellows 1, and photogenic bacterium microwell plate 2 is located at the top, axis of CCD digital camera 4, and CCD digital camera 4 links to each other with computer 5 by data line, in the computer 5 image processing software is arranged.Camera bellows 1 specification is 150 * 120 * 200 (mm), is made by the black unorganic glass of lucifuge.Photogenic bacterium microwell plate 2 is bilayer structures, and specification is 150 * 120 * 30 (mm), has 20 micropores, upper strata micro-pore diameter 12mm, and the micro-pore diameter 1mm of lower floor is made by the black unorganic glass of lucifuge.Reaction tank 3 is plastic centrifuge tubes of 5ml.CCD digital camera 4 has ISO, and ISO is more than 12800.When add the photogenic bacterium sample liquid in reaction tank 3 after, the variation of bacterial fluorescence will be caught by CCD digital camera 4, after data line imports computer 5 into, change numerary signal into, be analyzed by the software in the computer 5 again.
The beneficial effects of the utility model are: have portability, rapid determination, flux height, characteristics simple to operate, can satisfy the demand of the rapid detection of the on-the-spot a large amount of samples of sudden marime fouling disaster, for the portable seawater comprehensive toxicity rapid detection apparatus of development based on the bacterial luminescence imaging technique lays the foundation.
Description of drawings
Below in conjunction with the drawings and specific embodiments the utility model is further specified.
Fig. 1 is principle framework figure of the present utility model.
Fig. 2 is a structural representation of the present utility model.
Among the figure: 1, by camera bellows, 2, the photogenic bacterium microwell plate, 3, reaction tank, 4, the CCD digital camera, 5, computer.
Specific embodiments
As shown in Figure 2, the utility model is made up of camera bellows 1, photogenic bacterium microwell plate 2, reaction tank 3, CCD digital camera 4 and computer 5.CCD digital camera 4 in camera bellows 1 below, photogenic bacterium microwell plate 2 is above the axis of CCD digital camera 4, CCD digital camera 4 links to each other with computer 5 by data line, in the computer 5 image processing software is arranged.Camera bellows 1 specification is 150 * 120 * 200 (mm), is made by the black unorganic glass of lucifuge.Photogenic bacterium microwell plate 2 is bilayer structures, and specification is 150 * 120 * 30 (mm), has 20 micropores, upper strata micro-pore diameter 12mm, and the micro-pore diameter 1mm of lower floor is made by the black unorganic glass of lucifuge.Reaction tank 3 is plastic centrifuge tubes of 5ml.The capable ISO of CCD digital camera 4 tools, ISO is 12800.When add the photogenic bacterium sample liquid in reaction tank 3 after, the variation of bacterial fluorescence will be caught by CCD digital camera 4, after data line imports computer 5 into, change numerary signal into, be analyzed by the software in the computer 5 again.
Claims (5)
1, a kind of portable CCD imaging photogenic bacterium proofing unit is characterized in that, this device is made up of camera bellows (1), photogenic bacterium microwell plate (2), reaction tank (3), CCD digital camera (4) and computer (5); CCD digital camera (4) is located at the below in the camera bellows (1), photogenic bacterium microwell plate (2) is located at the top, axis of CCD digital camera (4), CCD digital camera (4) links to each other with computer (5) by data line, and computer has image processing software in (5).
2, a kind of portable CCD imaging photogenic bacterium proofing unit according to claim 1 is characterized in that described camera bellows (1) specification is 150mm * 120mm * 200mm, is made by the black unorganic glass of lucifuge.
3, a kind of portable CCD imaging photogenic bacterium proofing unit according to claim 1, it is characterized in that, described photogenic bacterium microwell plate (2) is a bilayer structure, specification is 150mm * 120mm * 30mm, have 20 micropores, upper strata micro-pore diameter 12mm, the micro-pore diameter 1mm of lower floor is made by the black unorganic glass of lucifuge.
4, a kind of portable CCD imaging photogenic bacterium proofing unit according to claim 1 is characterized in that described reaction tank (3) is the plastic centrifuge tube of 5ml.
5, a kind of portable CCD imaging photogenic bacterium proofing unit according to claim 1 is characterized in that described CCD digital camera (4) ISO ISO is more than 12800.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009200145927U CN201433218Y (en) | 2009-06-12 | 2009-06-12 | Portable CCD imaging detecting device for luminescent bacterium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009200145927U CN201433218Y (en) | 2009-06-12 | 2009-06-12 | Portable CCD imaging detecting device for luminescent bacterium |
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| Publication Number | Publication Date |
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| CN201433218Y true CN201433218Y (en) | 2010-03-31 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2009200145927U Expired - Fee Related CN201433218Y (en) | 2009-06-12 | 2009-06-12 | Portable CCD imaging detecting device for luminescent bacterium |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102676381A (en) * | 2011-03-18 | 2012-09-19 | 上海华勤通讯技术有限公司 | Mobile terminal with bacteria detection function |
| CN103983645A (en) * | 2014-05-11 | 2014-08-13 | 王新华 | Novel medical equipment bacteria tester |
| CN105176803A (en) * | 2015-10-09 | 2015-12-23 | 浙江省海洋水产研究所 | Portable microbial limit detector for seafood |
| CN115046922A (en) * | 2022-03-31 | 2022-09-13 | 重庆大学 | Portable water quality comprehensive toxicity analyzer based on luminous bacteria and detection method |
-
2009
- 2009-06-12 CN CN2009200145927U patent/CN201433218Y/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102676381A (en) * | 2011-03-18 | 2012-09-19 | 上海华勤通讯技术有限公司 | Mobile terminal with bacteria detection function |
| CN103983645A (en) * | 2014-05-11 | 2014-08-13 | 王新华 | Novel medical equipment bacteria tester |
| CN103983645B (en) * | 2014-05-11 | 2017-05-17 | 赵晓莉 | Novel medical equipment bacteria tester |
| CN105176803A (en) * | 2015-10-09 | 2015-12-23 | 浙江省海洋水产研究所 | Portable microbial limit detector for seafood |
| CN115046922A (en) * | 2022-03-31 | 2022-09-13 | 重庆大学 | Portable water quality comprehensive toxicity analyzer based on luminous bacteria and detection method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20100331 Termination date: 20100612 |