CN201342326Y - Micromixer for digital droplets - Google Patents
Micromixer for digital droplets Download PDFInfo
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- CN201342326Y CN201342326Y CNU2009201127697U CN200920112769U CN201342326Y CN 201342326 Y CN201342326 Y CN 201342326Y CN U2009201127697 U CNU2009201127697 U CN U2009201127697U CN 200920112769 U CN200920112769 U CN 200920112769U CN 201342326 Y CN201342326 Y CN 201342326Y
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- digital
- reflecting grating
- lead
- interdigital transducer
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Abstract
The utility model discloses a micromixer for digital droplets, which comprises an object stage, a piezoelectrical substrate, a glass slide and a movable bracket, wherein, a cavity is formed on the loading platform; the piezoelectrical substrate is arranged in the cavity; the lower surface of the piezoelectrical substrate is a working surface; a first reflecting grating and a second reflecting grating, an interdigital transducer, a first hydrophobic layer used for mixing two digital droplets are arranged on the working surface; the first reflecting grating and the second reflecting grating are respectively arranged at the two ends of the working surface; the interdigital transducer is close to the first reflecting grating; the first hydrophobic layer is positioned between the interdigital transducer and the second reflecting grating; a second hydrophobic layer used for putting the two digital droplets to be mixed is arranged on the upper surface of the glass slide; and the thickness of the second hydrophobic layer is larger than that of the first hydrophobic layer. The utility model has the advantages that the two digital droplets which are put on a storage area are attached to a mixed working area, so that the purpose that the two digital droplets are mixed on the working surface of the hung piezoelectrical substrate is achieved, and the spatial flexibility of the manipulation of the digital droplets is improved.
Description
Technical field
The utility model relates to the microfluid micro-mixer in a kind of micro-fluidic chip, especially relates to a kind of digital microfluid micro-mixer.
Background technology
General bio-medical analysis comprises basic processes such as sample pretreatment, mixing, reaction, separation and detection, these basic processes are integrated in several square centimeters of chips, to replace traditional laboratory work, claim that it is laboratory on the sheet (Lab-on-a-chip) or micro-total analysis system (Micro Total Analytical System).The laboratory is few, simple to operate owing to having amount of samples on the sheet, and can accurately finish the overall process that from the sample preparation to result, shows within a short period of time, can overcome the experimental error that manual operations brings in traditional laboratory work effectively, in chemical analysis, dna sequencing, biology and chemical sensitisation, molecular separation, nucleic acid ordering and fields such as analysis, medical diagnosis on disease, drug screening, environmental monitoring and national security, more and more be used.
Microfluid mixer is breadboard important operation parts on the sheet, and has determined the reliability and the credibility of subsequent analysis work to a great extent.Microfluid mixer is not only the indispensable functional part in laboratory on the sheet, and in chemical synthesis, emulsion preparation, high flux screening and other biochemical fields important application prospects is arranged all.In recent years, a lot of scholars have carried out extensive studies to microfluid mixer, and proposed many multi-form, based on the microfluid mixer of different principle.These microfluid mixers add driver and can roughly be divided into active and passive type two classes according to having or not.Active mode mixer for microflow mainly is to realize married operation to sample by adding the field of force.Proposed in recent years to adopt to add magnetic, electricity, sound equal excitation, improved the mixing efficiency of microfluid to produce the motion of PERIODIC INTERFERENCE flow field acceleration microfluid.Passive microfluid mixer is used the free diffusion mechanism of microfluid, adopts the microchannel of special shape or the special construction in the microchannel, to produce horizontal mass transport or to make the flow field be in disordered state to quicken the mixing of microfluid.This two classes microfluid mixer has improved the mixed effect of microfluid to a certain extent, but has simultaneously that incorporation time is long, technology is complicated, needs shortcomings such as quantity of sample consumption and chip occupying area are bigger.
For solving the shortcoming that above-mentioned two class microfluid mixers exist, the correlative study personnel have proposed the digital micro-fluid blender, the digital micro-fluid blender can be realized quick biochemical reaction in the process of mixing microfluid, it is integrated to make that the digital micro-fluid blender is more suitable for, has bigger development prospect, to be the following important developing direction in laboratory on the sheet, be subjected to domestic and international expert's great attention.2003 the 3rd volumes of periodical " laboratory on the sheet " the 1st phase 28-33 page or leaf (Labon a chip Vol.3 (1), " being used for micro-fluidic system based on the wetting little drop micro-mixer of electricity " (" Electrowetting-based droplet mixers for microfluidic systems ") 2003:28-33) disclosed, this is a kind of digital microfluid micro-mixer based on the wetting little drop micro-mixer of electricity, it adopts three-decker, comprise top crown and bottom crown, prop with small cushion block between top crown and the bottom crown, little drop is clamped between top crown and the bottom crown, top crown mainly is made up of clear glass, on clear glass, be deposited with transparent electrode layer such as ITO (Indium Tin Oxides, the nano indium tin metal oxide), on transparent electrode layer, apply one deck hydrophobic material and form hydrophobic film, with transparent electrode layer as ground electrode, bottom crown mainly is made up of substrate, deposit and make microelectrode array by lithography in the substrate, and coating one deck hydrophobic material forms hydrophobic film on microelectrode array, by changing the voltage between microelectrode array and the transparent electrode layer, realize motion and mixing in the two-dimensional plane of digital micro-fluid between top crown and bottom crown to be mixed.This digital microfluid micro-mixer incorporation time is shorter, but because integrated microelectrode array needs configurating programmable microelectrode on-off control system, the realization microfluidic manipulations is had relatively high expectations; In addition, top crown in this micro-mixer and bottom crown are an indivisible integral body, digital micro-fluid can only mix in the two-dimensional plane of top crown and bottom crown, limited its integrated level in the laboratory and to the space flexibility of digital microfluidic manipulations on sheet.
Summary of the invention
Technical problem to be solved in the utility model provides a kind of space flexibility that can effectively improve digital microfluidic manipulations, and can effectively improve the digital microfluid micro-mixer of laboratory integrated level on the sheet.
The utility model solves the problems of the technologies described above the technical scheme that is adopted: a kind of digital microfluid micro-mixer, comprise objective table, piezoelectric substrate, glass slide and the travel(l)ing rest that is used to put described glass slide, described objective table is provided with cavity, described piezoelectric substrate is arranged in the described cavity, the lower surface of described piezoelectric substrate is a working surface, described working surface is provided with first reflecting grating, second reflecting grating, interdigital transducer and first hydrophobic layer that is used for two digital Microfluidic Mixing work, described first reflecting grating and described second reflecting grating are separately positioned on the two ends of described working surface, the position of described interdigital transducer is near the position of described first reflecting grating, described interdigital transducer is connected with the external signal generating means, described first hydrophobic layer is between described interdigital transducer and described second reflecting grating, the upper surface of described glass slide is provided with second hydrophobic layer that is used to place two digital micro-fluids to be mixed, and the thickness of described second hydrophobic layer is greater than the thickness of described first hydrophobic layer.
Described piezoelectric substrate is optical grade LiNbO
3Piezoelectric substrate.
The periphery of described piezoelectric substrate is by the inwall bonding connection of existing adhesive and described cavity.
Described first reflecting grating, described second reflecting grating and described interdigital transducer all adopt existing microelectronic technique to be produced on the described working surface.
Described signal generation apparatus comprises the signal generator and the power amplifier that is connected with described signal generator that is used to produce the RF signal of telecommunication, and described power amplifier is connected with described interdigital transducer.
Described interdigital transducer comprises two busbars, and described objective table is provided with terminal pin, is connected by first lead between described busbar and the described terminal pin, is connected by second lead between described terminal pin and the described power amplifier.
Described first lead is spun gold or filamentary silver, one end of described spun gold or described filamentary silver is connected on the described busbar by existing bond technology, described second lead is general lead, and an end of described general lead is welded on the described terminal pin.
Described first lead is spun gold or filamentary silver, one end of described spun gold or described filamentary silver is connected on the described busbar by existing conductive silver glue, described second lead is general lead, and an end of described general lead is welded on the described terminal pin.
Described travel(l)ing rest is at level and all adjustable support of vertical direction.
Compared with prior art, advantage of the present utility model is to be attached in the hybrid working district of piezoelectric substrate working surface by two digital micro-fluids that store in the district that will be placed in glass slide, realize two digital micro-fluids rapid mixing on the working surface of unsettled piezoelectric substrate, improved space yarage digital micro-fluid; Simultaneously owing to be used for storing the glass slide of digital micro-fluid and can be any and go up the arbitrary region of laboratory after hydrophobic treatment, therefore on a sheet, become possibility in the laboratory for integrated each operating unit as much as possible, greatly improved breadboard integrated level on the sheet; Micro-mixer of the present utility model can be integrated on the sheets such as medical diagnosis on disease, drug screening, environmental monitoring and national security in the laboratory.
Description of drawings
Fig. 1 is the structural representation of digital microfluid micro-mixer of the present utility model.
The specific embodiment
Embodiment describes in further detail the utility model below in conjunction with accompanying drawing.
As shown in the figure, a kind of digital microfluid micro-mixer, comprise (Printed Circuie Board by PCB, printed substrate) objective table 1 that is made into of plate, piezoelectric substrate 2, glass slide 4 and the travel(l)ing rest 9 that is used to put glass slide 4, objective table 1 is provided with cavity 11, piezoelectric substrate 2 is arranged in the cavity 11, the lower surface of piezoelectric substrate 2 is a working surface 21, in actual application, can be directly with the inwall bonding connection of the periphery of piezoelectric substrate 2 by existing any adhesive and cavity 11, also can adopt other modes that piezoelectric substrate 2 is arranged in the cavity 11.Working surface 21 is provided with first reflecting grating 51, second reflecting grating 52, interdigital transducer 6 and first hydrophobic layer 3 that is used for two digital Microfluidic Mixing work, first hydrophobic layer 3 is to apply one deck TeflonAF 1600 hydrophobic materials to form on working surface 21, first reflecting grating 51 and second reflecting grating 52 are separately positioned on the two ends of working surface 21, the position of interdigital transducer 6 is near the position of first reflecting grating 51, position between the interdigital transducer 6 and second reflecting grating 52 forms an acoustic propagation path like this, in working order down, the surface acoustic wave that interdigital transducer 6 produces is propagated by the acoustic propagation path, and interdigital transducer 6 is connected with external signal generating means 10.First hydrophobic layer 3 is between the interdigital transducer 6 and second reflecting grating 52, and promptly first hydrophobic layer 3 is positioned on the acoustic propagation path, and first hydrophobic layer 3 is configured for mixing the hybrid working district of two digital micro-fluids 7 to be mixed.The upper surface of glass slide 4 is provided with second hydrophobic layer 8, second hydrophobic layer 8 is to apply one deck Teflon AF 1600 hydrophobic materials to form on the upper surface of glass slide 4, the digital micro-fluid that second hydrophobic layer 8 is configured for placing two digital micro-fluids 7 to be mixed stores the district, the lower surface of glass slide 4 contacts with travel(l)ing rest 9, and regulating down travel(l)ing rest 9 in working order, to guarantee that digital micro-fluid stores the position in district corresponding with the position in hybrid working district.At this, need to regulate the thickness of the hydrophobic material that applies, the thickness of second hydrophobic layer 8 must be greater than the thickness of first hydrophobic layer 3, can make the hydrophobicity of second hydrophobic layer 8 better like this than the hydrophobicity of first hydrophobic layer 3, because hydrophobic performance is good more, surface tension is just more little, and therefore the surface tension of first hydrophobic layer 3 can be attached in the hybrid working district being seated in two digital micro-fluids to be mixed that store in the district greater than the surface tension of second hydrophobic layer 8 as can be known.
In this specific embodiment, objective table 1 also can be made by other materials; Piezoelectric substrate 2 adopts optical grade LiNbO
3Piezoelectric substrate; First reflecting grating 51, second reflecting grating 52 and interdigital transducer 6 all adopt existing microelectronic technique to be produced on the working surface 21; Signal generation apparatus 10 comprises the signal generator 101 and the power amplifier 102 that is connected with signal generator 101 that is used to produce the RF signal of telecommunication, and power amplifier 102 is connected with interdigital transducer 6; Interdigital transducer 6 comprises two busbars 61, objective table 1 is provided with terminal pin 12, be connected by first lead 62 between busbar 61 and the terminal pin 12, be connected by second lead 63 between terminal pin 12 and the power amplifier 102, first lead 62 can be spun gold or filamentary silver, one end of spun gold or filamentary silver is connected on the busbar 61 by bond technology, and an end of spun gold or filamentary silver also can be connected on the busbar 61 by existing conductive silver glue; First lead 62 also can be general thin wire, different with spun gold or filamentary silver is, one end of general thin wire is bonded on the busbar 61 by existing conductive silver glue, and it is common connection lead that second lead 63 adopts general lead, and an end of second lead 63 is welded on the terminal pin 12.
In this specific embodiment, travel(l)ing rest can adopt having at level and all adjustable support of vertical direction of any existing maturation.
Utilize above-mentioned micro-mixer to realize that the process of digital micro-fluid mixing is as follows:
1. constitute digital micro-fluid by second hydrophobic layer that is used to place two digital micro-fluids to be mixed and store the district, the hybrid working district that constitutes by first hydrophobic layer that is used for two digital Microfluidic Mixing work, adopting existing microsyringe that two digital micro-fluids to be mixed are pipetted into digital micro-fluid stores in the district, and the SAW propagating path almost parallel that produces when keeping the line of two digital micro-fluids to be mixed and interdigital transducer work is so that the operation of follow-up Microfluidic Mixing;
2. whole glass slide is placed on the travel(l)ing rest, then travel(l)ing rest is placed in the objective table below;
3. adjusted travel(l)ing rest in the horizontal direction makes the position in the hybrid working district that digital micro-fluid position that stores the district and first hydrophobic layer that is used for two digital Microfluidic Mixing work constitute corresponding;
4. in vertical direction to the adjusted travel(l)ing rest, two digital micro-fluids to be mixed are contacted with the surface in hybrid working district;
5. because the tension force on the surface in the hybrid working district on the surface that stores the district and the piezoelectric substrate on the glass slide is different, therefore two digital micro-fluids to be mixed can be attached on the surface in hybrid working district, be attached on the surface in hybrid working district when two digital micro-fluids to be mixed after, regulate travel(l)ing rest in vertical direction gradually downwards, two digital micro-fluids to be mixed are broken away from store the district, remove travel(l)ing rest then;
6. at this moment, start signal generator and power amplifier, signal generator produces the RF signal of telecommunication, and the RF signal of telecommunication is transferred to power amplifier, power amplifier amplifies the RF signal of telecommunication handles the RF signal of telecommunication that obtains amplifying, interdigital transducer inserts the RF signal of telecommunication that amplifies by second lead and first lead, and interdigital transducer produces surface acoustic wave behind the access RF signal of telecommunication; The frequency of this RF signal of telecommunication is the sound synchronizing frequency of interdigital transducer, and the intensity of this RF signal of telecommunication can be selected suitable intensity according to actual conditions;
7. the surface acoustic wave driving is moved to the digital micro-fluid to be mixed away from interdigital transducer one side near the digital micro-fluid to be mixed of interdigital transducer one side, and two digital micro-fluids to be mixed are merged and rapid mixing.
Because digital micro-fluid energy absorption to be mixed near interdigital transducer one side, and surface acoustic wave is depleted in communication process and the surface acoustic wave of second reflecting grating reflection arrival, second reflecting grating, so when the RF signal of telecommunication that inserts is not too big, keep substantially not moving away from the digital micro-fluid to be mixed of interdigital transducer one side.
Claims (9)
1, a kind of digital microfluid micro-mixer, it is characterized in that comprising objective table, piezoelectric substrate, glass slide and the travel(l)ing rest that is used to put described glass slide, described objective table is provided with cavity, described piezoelectric substrate is arranged in the described cavity, the lower surface of described piezoelectric substrate is a working surface, described working surface is provided with first reflecting grating, second reflecting grating, interdigital transducer and first hydrophobic layer that is used for two digital Microfluidic Mixing work, described first reflecting grating and described second reflecting grating are separately positioned on the two ends of described working surface, the position of described interdigital transducer is near the position of described first reflecting grating, described interdigital transducer is connected with the external signal generating means, described first hydrophobic layer is between described interdigital transducer and described second reflecting grating, the upper surface of described glass slide is provided with second hydrophobic layer that is used to place two digital micro-fluids to be mixed, and the thickness of described second hydrophobic layer is greater than the thickness of described first hydrophobic layer.
2, a kind of digital microfluid micro-mixer according to claim 1 is characterized in that described piezoelectric substrate is optical grade LiNbO
3Piezoelectric substrate.
3, a kind of digital microfluid micro-mixer according to claim 1 is characterized in that the inwall bonding connection of the periphery of described piezoelectric substrate by existing adhesive and described cavity.
4, a kind of digital microfluid micro-mixer according to claim 1 is characterized in that described first reflecting grating, described second reflecting grating and described interdigital transducer all adopt existing microelectronic technique to be produced on the described working surface.
5, a kind of digital microfluid micro-mixer according to claim 1, it is characterized in that described signal generation apparatus comprises the signal generator and the power amplifier that is connected with described signal generator that is used to produce the RF signal of telecommunication, described power amplifier is connected with described interdigital transducer.
6, a kind of digital microfluid micro-mixer according to claim 5, it is characterized in that described interdigital transducer comprises two busbars, described objective table is provided with terminal pin, be connected by first lead between described busbar and the described terminal pin, be connected by second lead between described terminal pin and the described power amplifier.
7, a kind of digital microfluid micro-mixer according to claim 6, it is characterized in that described first lead is spun gold or filamentary silver, one end of described spun gold or described filamentary silver is connected on the described busbar by existing bond technology, described second lead is general lead, and an end of described general lead is welded on the described terminal pin.
8, a kind of digital microfluid micro-mixer according to claim 6, it is characterized in that described first lead is spun gold or filamentary silver, one end of described spun gold or described filamentary silver is connected on the described busbar by existing conductive silver glue, described second lead is general lead, and an end of described general lead is welded on the described terminal pin.
9, a kind of digital microfluid micro-mixer according to claim 1 is characterized in that described travel(l)ing rest is at level and all adjustable support of vertical direction.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2009201127697U CN201342326Y (en) | 2009-01-15 | 2009-01-15 | Micromixer for digital droplets |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2009201127697U CN201342326Y (en) | 2009-01-15 | 2009-01-15 | Micromixer for digital droplets |
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| Publication Number | Publication Date |
|---|---|
| CN201342326Y true CN201342326Y (en) | 2009-11-11 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNU2009201127697U Expired - Lifetime CN201342326Y (en) | 2009-01-15 | 2009-01-15 | Micromixer for digital droplets |
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| CN (1) | CN201342326Y (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101497006B (en) * | 2009-01-15 | 2011-04-20 | 宁波大学 | Digital microfluid micro-mixer and mixing method |
| CN109772481B (en) * | 2017-11-14 | 2021-05-18 | 上准微流体股份有限公司 | Fluid connector, microfluidic chip cartridge and fluid connection assembly |
-
2009
- 2009-01-15 CN CNU2009201127697U patent/CN201342326Y/en not_active Expired - Lifetime
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101497006B (en) * | 2009-01-15 | 2011-04-20 | 宁波大学 | Digital microfluid micro-mixer and mixing method |
| CN109772481B (en) * | 2017-11-14 | 2021-05-18 | 上准微流体股份有限公司 | Fluid connector, microfluidic chip cartridge and fluid connection assembly |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20091111 Effective date of abandoning: 20090115 |