CN209846060U

CN209846060U - Sperm cryoculture slide glass

Info

Publication number: CN209846060U
Application number: CN201920522498.6U
Authority: CN
Inventors: 冯科
Original assignee: Individual
Current assignee: Individual
Priority date: 2019-04-17
Filing date: 2019-04-17
Publication date: 2019-12-27
Anticipated expiration: 2029-04-17

Abstract

The utility model belongs to the field of medical equipment, in particular to a sperm freezing culture slide glass, which comprises a freezing slide glass, a cut-off component and a sealing cover, wherein the freezing slide glass is provided with a stock solution pool and a collecting pool, a communicating hole is arranged between the stock solution pool and the collecting pool, the freezing slide glass is also provided with a step hole, the step hole is communicated with the communicating hole, and the cut-off component is arranged in the step hole; during the letter sorting, it is sealed that at first dams through the component that dams with the intercommunicating pore, will handle the back seminal fluid and put into the stock solution pond of freezing slide glass, add the diluent in collecting the pond, place a period, slowly open the component that dams, make the intercommunicating pore link up, at this moment, the activity sperm can be constantly moved, and the sperm easily swims into the collecting pit, realizes the purpose of sperm letter sorting, effectively prevents simultaneously at the in-process of adding the diluent, because the reason of the velocity of flow leads to the seminal fluid in the stock solution pond to pass through the intercommunicating pore backward flow, improves sperm letter sorting quality.

Description

Sperm cryoculture slide glass

Technical Field

The utility model relates to the field of medical equipment, in particular to a sperm freezing culture slide glass.

Background

In vitro fertilization-embryo transfer is also called as a test tube infant, because factors such as ice crystal formation in a cell in the sperm freezing process can cause sperm damage to a certain degree, and in addition, mechanical damage is caused when the sperm is manually selected after unfreezing, the number of the sperm with vitality can be greatly reduced, time and labor are wasted, the sperm can only be stored for the freezing of the rare sperm, the recovery rate is not high based on the freezing and storing of the rare sperm at present, even after a couple of a patient enters the week, the number of the movable sperm found by recovery is smaller than that of the ova obtained by a female, and the movable sperm can not be found after the sperm of some patients are frozen and recovered.

Chinese patent publication No. CN208586284U discloses a preferred sperm cryoculture slide. This patent technique can autosegregation activity sperm and immortal sperm, avoids the mechanical damage of artifical selection, and especially to rare sperm patient's seminal fluid, can increase the quantity of finding activity sperm to can directly be used for liquid nitrogen cryopreservation, however, when it adds the diluent, because the reason of velocity of flow can lead to the seminal fluid in the stoste pond to pass through the intercommunicating pore backward flow, lead to the selection quality of sperm to be lower. Accordingly, a sperm cell culture slide has been provided to solve the above-mentioned problems of the background art by those skilled in the art.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a sperm cryoculture slide glass to solve the problem that proposes among the above-mentioned background art.

In order to achieve the above object, the utility model provides a following technical scheme:

a sperm freeze culture slide glass comprises a frozen slide glass, a cut-off component and a sealing cover, wherein a stock solution pool and a collecting pool are arranged on the frozen slide glass, a communicating hole is formed between the stock solution pool and the collecting pool, a step hole is further formed in the frozen slide glass and communicated with the communicating hole, the cut-off component is installed in the step hole and comprises a sealing column and a connecting plate fixed on the top surface of the sealing column, the sealing column is connected to the inner wall of the step hole in a sliding mode, a screw rod is connected to the top surface of the connecting plate in a rotating mode, a fixing plate is fixedly connected to the step surface of the step hole, the screw rod is in threaded connection with the fixing plate, a handle is fixedly connected to the top end of the fixing plate, and the sealing cover.

As a further aspect of the present invention: the bottom surface of the frozen slide glass is provided with a containing cavity, the inner wall of the containing cavity is in threaded connection with a threaded column, the top surface of the threaded column is fixedly connected with a containing vessel, and the top end of the inner wall of the containing cavity is fixedly bonded with a sealing ring.

As a further aspect of the present invention: the two ends of the bottom surface of the frozen slide are fixedly connected with anti-slip rubber strips, and the side wall of the frozen slide is also fixedly connected with a clamping plate.

As a further aspect of the present invention: the medial surface fixedly connected with stoste pond sealing plug and the collecting pit sealing plug of sealed lid, just stoste pond sealing plug with the size and the position of collecting pit sealing plug respectively with stoste pond with the collecting pit is corresponding.

As a further aspect of the present invention: one end of the inner side wall of the sealing cover is fixedly connected with a locking screw, the locking screw is in threaded connection with a locking ring, and the size and the position of the locking screw correspond to those of the clamping plate.

As a further aspect of the present invention: the material of stock solution pond sealing plug, the collecting pit sealing plug with the sealed post is rubber.

As a further aspect of the present invention: the cardboard is "U" font.

Compared with the prior art, the beneficial effects of the utility model are that:

1. at first through the component that dams with the intercommunicating pore sealed, put into the stock solution pond of freezing slide glass with handling back seminal fluid, add the diluent in the collecting tank, place a period, slowly open the component that dams, make the intercommunicating pore link up, at this moment, the activity sperm can be constantly moving, and the collecting tank is gone into to the easy trip of sperm, is difficult for wandering out, realizes the purpose of sperm letter sorting, effectively prevents simultaneously at the in-process that adds the diluent, because the reason of the velocity of flow leads to the seminal fluid in the stock solution pond to pass through the intercommunicating pore backward flow, improves sperm letter sorting quality.

2. When the frozen slide glass needs to be stored, the sealing cover can be closed, the stock solution pool sealing plug is sealed to the stock solution pool, and the collecting pool sealing plug is used for sealing the collecting pool, so that the frozen slide glass is convenient to store for a long time.

3. When the needs take out the slide glass that freezes and use, can be to holding the ware filling liquid nitrogen to install to holding the intracavity through the screw thread post, and sealed by the sealing washer, sustainable cooling to freezing slide glass, guarantee that the inside sperm of freezing slide glass can not go bad.

Drawings

FIG. 1 is a schematic diagram of a sperm cryoculture slide;

FIG. 2 is a schematic diagram of the internal structure of a sperm cryoculture slide;

FIG. 3 is a schematic view of a shut-off member in a sperm cryoculture slide;

FIG. 4 is a schematic view of a locking screw structure in a slide for frozen sperm culture;

FIG. 5 is a schematic diagram of a clamping plate in a sperm cell culture slide.

In the figure: 1. freezing the slide; 101. a stock solution tank; 102. a collection tank; 103. a communicating hole; 104. a stepped bore; 105. a containing cavity; 11. an anti-slip rubber strip; 12. a threaded post; 13. a containing vessel; 14. a seal ring; 15. clamping a plate; 2. a closure member; 21. sealing the column; 22. a connecting plate; 23. a screw; 24. a fixing plate; 25. a handle; 3. a sealing cover; 31. sealing a stock solution pool; 32. a collecting tank sealing plug; 33. locking the screw rod; 34. and (4) locking the ring.

Detailed Description

Please refer to fig. 1-5, in the embodiment of the present invention, a slide glass for frozen sperm culture, including frozen slide glass 1, cut-off member 2 and sealing cover 3, frozen slide glass 1 has been seted up stoste pond 101 and collecting pit 102, intercommunicating pore 103 has been seted up between stoste pond 101 and collecting pit 102, frozen slide glass 1 has still been seted up step hole 104, step hole 104 is link up with intercommunicating pore 103, install cut-off member 2 in step hole 104, cut-off member 2 includes sealed post 21 and fixes the connecting plate 22 at its top surface, sealed post 21 sliding connection is in the inner wall of step hole 104, the top surface of connecting plate 22 rotates and is connected with screw 23, the step face fixedly connected with fixed plate 24 in step hole 104, screw 23 and fixed plate 24 threaded connection, the top fixedly connected with handle 25 of fixed plate 24, the top surface of frozen slide.

In fig. 1: the frozen slide glass 1 is provided with a stock solution tank 101 and a collecting tank 102, a communicating hole 103 is formed between the stock solution tank 101 and the collecting tank 102, the frozen slide glass 1 is further provided with a step hole 104, the top surface of the frozen slide glass 1 is hinged with a sealing cover 3, the stock solution tank 101 stores the frozen stock solution, and the collecting tank 102 can store the sorted stock solution and is sealed through the sealing cover 3.

In fig. 2: the frozen slide 1 is provided with a stock solution pool 101 and a collecting pool 102, a communicating hole 103 is arranged between the stock solution pool 101 and the collecting pool 102, the frozen slide 1 is also provided with a step hole 104, the step hole 104 is communicated with the communicating hole 103, a cut-off component 2 is arranged in the step hole 104, firstly, the communicating hole 103 is cut off and sealed by the cut-off component 2, the processed semen is put into the stock solution pool 101 of the frozen slide 1, the liquid level is kept above the communicating hole 103, diluent is added into the collecting pool 102, the liquid level exceeds the communicating hole 103, the cut-off component 2 is placed for a period of time and is slowly opened to ensure the communicating hole 103 to be communicated, at the moment, the movable sperm can continuously move, the opening of the communicating hole 103 at the end of the collecting pool 102 is small, the opening at the end of the stock solution pool 101 is large, the sperm can easily swim into the collecting pool 102 and is not easy to swim out, the purpose of sorting the sperm is realized, and the, the sperm sorting quality is improved, the top surface of the frozen slide 1 is hinged with a sealing cover 3, the inner side surface of the sealing cover 3 is fixedly connected with a stock solution pool sealing plug 31 and a collecting pool sealing plug 32, and the sizes and the positions of the stock solution pool sealing plug 31 and the collecting pool sealing plug 32 respectively correspond to those of a stock solution pool 101 and a collecting pool 102. When the frozen slide glass 1 needs to be stored, the sealing cover 3 can be closed, the stock solution pool sealing plug 31 is enabled to seal the stock solution pool 101, the collection pool sealing plug 32 is enabled to seal the collection pool 102, long-term storage is facilitated, the bottom surface of the frozen slide glass 1 is provided with the containing cavity 105, the inner wall of the containing cavity 105 is in threaded connection with the threaded column 12, the top surface of the threaded column 12 is fixedly connected with the containing vessel 13, the top end of the inner wall of the containing cavity 105 is fixedly bonded with the sealing ring 14, when the frozen slide 1 needs to be taken out for use, the containing vessel 13 can be filled with liquid nitrogen, is installed in the containing cavity 105 through the threaded column 12 and is sealed by the sealing ring 14, the temperature of the frozen slide 1 can be continuously reduced, so that sperms in the frozen slide 1 can not go bad, and the anti-slip rubber strips 11 are fixedly connected to two ends of the bottom surface of the frozen slide 1, so that the frozen slide 1 can be prevented from sliding on the placement surface.

In fig. 3: the closure member 2 comprises a sealing column 21 and a connecting plate 22 fixed on the top surface of the sealing column 21, the sealing column 21 is slidably connected to the inner wall of the stepped hole 104, the top surface of the connecting plate 22 is rotatably connected with a screw 23, the stepped surface of the stepped hole 104 is fixedly connected with a fixing plate 24, the screw 23 is in threaded connection with the fixing plate 24, the top end of the fixing plate 24 is fixedly connected with a handle 25, the screw 23 is rotated through the handle 25, the sealing column 21 moves upwards or downwards under the action of threads, and sealing closure and opening of the communicating hole 103 are achieved.

In fig. 4: the side wall of the frozen slide 1 is also fixedly connected with a clamping plate 15, one end of the inner side wall of the sealing cover 3 is fixedly connected with a locking screw 33, the locking screw 33 is in threaded connection with a locking ring 34, the size and the position of the locking screw 33 correspond to those of the clamping plate 15, when the sealing cover 3 is closed, the locking screw 33 is clamped with the clamping plate 15, the locking ring 34 is rotated to lock the sealing cover 3, and the tightness of the sealing cover 3 is ensured.

In fig. 5: the clamping plate 15 is U-shaped, so that the locking screw 33 can be conveniently clamped with the clamping plate 15.

The utility model discloses a theory of operation is: the screw 23 is rotated by the handle 25, the sealing column 21 moves upwards or downwards under the action of the thread to realize the sealed cut-off and open communication of the communicating hole 103, firstly, the communicating hole 103 is cut-off and sealed by the cut-off component 2, the treated semen is placed in the stock solution pool 101 of the frozen slide 1, the liquid level is kept above the communicating hole 103, the diluent is added into the collecting pool 102, the liquid level exceeds the communicating hole 103, the cut-off component 2 is placed for a period of time, the communicating hole 103 is opened slowly, at the moment, the movable sperm can move continuously, the opening of the communicating hole 103 at the end of the collecting pool 102 is small, the opening at the end of the stock solution pool 101 is large, the sperm can easily swim into the collecting pool 102 and is not easy to swim out, the purpose of sperm sorting is realized, meanwhile, the semen in the stock solution pool 101 can be effectively prevented from flowing back through the communicating hole 103 due to, when needs are stored freezing slide glass 1, can close sealed lid 3, and make stoste pond sealing plug 31 sealed to stoste pond 101, collecting pit sealing plug 32 seals collecting pit 102, be convenient for store for a long time, when needs take out to freezing slide glass 1 and use, can be to holding 13 filling liquid nitrogen of ware, and install to holding in the chamber 105 through threaded column 12, and seal by sealing washer 14, the sustainable slide glass 1 that freezes cools down, guarantee that the inside sperm of freezing slide glass 1 can not go bad.

The above-mentioned, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, the concept of which is equivalent to replace or change, should be covered within the protection scope of the present invention.

Claims

1. The sperm freezing culture slide glass is characterized by comprising a freezing slide glass (1), a cut-off component (2) and a sealing cover (3), wherein the freezing slide glass (1) is provided with a stock solution pool (101) and a collecting pool (102), a communicating hole (103) is formed between the stock solution pool (101) and the collecting pool (102), the freezing slide glass (1) is further provided with a step hole (104), the step hole (104) is communicated with the communicating hole (103), the cut-off component (2) is arranged in the step hole (104), the cut-off component (2) comprises a sealing column (21) and a connecting plate (22) fixed on the top surface of the sealing column, the sealing column (21) is slidably connected to the inner wall of the step hole (104), the top surface of the connecting plate (22) is rotatably connected with a screw rod (23), and the step surface of the step hole (104) is fixedly connected with a fixing plate (24), the screw rod (23) is in threaded connection with the fixing plate (24), the top end of the fixing plate (24) is fixedly connected with a handle (25), and the top surface of the frozen slide (1) is hinged with the sealing cover (3).

2. The sperm freezing culture slide glass according to claim 1, wherein the bottom surface of the freezing slide glass (1) is provided with a containing cavity (105), the inner wall of the containing cavity (105) is connected with a threaded column (12) in a threaded manner, the top surface of the threaded column (12) is fixedly connected with a containing dish (13), and the top end of the inner wall of the containing cavity (105) is fixedly bonded with a sealing ring (14).

3. The sperm freezing culture slide as claimed in claim 1, wherein the two ends of the bottom surface of the freezing slide (1) are fixedly connected with anti-slip rubber strips (11), and the side wall of the freezing slide (1) is also fixedly connected with a clamping plate (15).

4. A slide for frozen sperm culture as claimed in claim 1, wherein the inner side of the sealing cover (3) is fixedly connected with a stock solution pool sealing plug (31) and a collection pool sealing plug (32), and the sizes and positions of the stock solution pool sealing plug (31) and the collection pool sealing plug (32) correspond to the stock solution pool (101) and the collection pool (102), respectively.

5. A slide as claimed in claim 3, wherein a locking screw (33) is fixedly connected to one end of the inner side wall of the sealing cover (3), a locking ring (34) is threadedly connected to the locking screw (33), and the locking screw (33) is sized and positioned to correspond to the clamping plate (15).

6. The slide glass for frozen sperm culture as claimed in claim 4, wherein the sealing plug (31) of the stock solution pool, the sealing plug (32) of the collection pool and the sealing column (21) are made of rubber.

7. A sperm cell freezing slide as claimed in claim 3, wherein said clamping plate (15) is "U" shaped.