CN209790967U - A device for extracting mononuclear cells from blood - Google Patents

Abstract

The embodiment of the utility model discloses a device for extracting mononuclear cells in blood, which comprises a tube body, a piston and a push rod, wherein the push rod is in threaded connection with the tube body, and the piston is fixedly connected with the push rod; the pipe body is divided into an upper pipe area, an extraction area and a lower pipe area, and the extraction area is positioned between the upper pipe area and the lower pipe area; the piston is positioned on the inner side of the lower pipe area and is in sealed sliding connection with the inner wall of the lower pipe area; one end of the push rod, which is far away from the piston, is positioned outside the lower pipe area, and the push rod drives the piston to move along the axial direction of the lower pipe area when rotating relative to the pipe body; one end of the upper pipe area, which is far away from the lower pipe area, is provided with a first jack, and the inner space of the upper pipe area is communicated with the outer space through the first jack; the extraction area is provided with an air hole, and the internal space of the extraction area is communicated with the external space through the air hole; its advantages are high sucking efficiency, isolating external air, no need of external solution, and no pollution to blood.

Description

A device for extracting mononuclear cells from blood

technical field

The embodiment of the utility model relates to the technical field of medical equipment, in particular to a device for extracting mononuclear cells in blood.

Background technique

In some medical testing and experimental processes, blood mononuclear cells need to be used as materials. Usually, blood and tissue fluid are separated first, and fresh blood (umbilical cord blood) is centrifuged to obtain plasma rich in mononuclear cells. Rich in a variety of growth factors, as nutrients for autologous cells, monocytes in peripheral blood or umbilical cord blood have been developed for the clinical adjuvant therapy of tumor immunity, and mononuclear cells in umbilical cord blood can also be used as a large number of blood/red blood cells origin of. The method is that after separation, it is first frozen, and when needed, it is further cultivated in large quantities and given therapeutic properties before being reinfused into the original body.

However, the current separation method is usually to use a syringe or blood collection tube to collect blood, and then send it to a sterile laboratory for centrifugation. During the separation process, it may be mixed with other external solutions and centrifuged. The operating device is not closed, and it is easy to contaminate during the process. The safety and environmental requirements of the external solution are strict, and the centrifugation operation of blood collection must be completed in different devices. Combining the above-mentioned processes, it is difficult to guarantee the quality of mononuclear cell-rich plasma.

Utility model content

For this reason, the embodiment of the utility model provides a device for extracting mononuclear cells in the blood, which does not need to be mixed with other external solutions during the process, reduces the chance of contact with the outside air or solution, reduces pollution, and improves the concentration of mononuclear cells. Efficiency and safety of separation from blood.

In order to achieve the above object, the utility model embodiment provides the following technical solutions:

A device for extracting mononuclear cells in blood, comprising a tube body, a piston and a push rod, the push rod is threadedly connected to the tube body, and the piston is fixedly connected to the push rod;

The pipe body is divided into an upper pipe area, an extraction area and a lower pipe area, and the extraction area is located between the upper pipe area and the lower pipe area;

The piston is located inside the lower tube area, and the piston is in sealing and sliding connection with the inner wall of the lower tube area;

The end of the push rod away from the piston is located outside the lower tube area, and when the push rod rotates relative to the tube body, it drives the piston to move axially along the lower tube area;

The end of the upper pipe area far away from the lower pipe area is provided with a first jack, and the inner space of the upper pipe area communicates with the external space through the first jack;

Air holes are opened on the extraction area, and the inner space of the extraction area communicates with the outer space through the air holes.

The embodiment of the utility model is further set as: the end of the upper tube area away from the lower tube area is rotatably connected with an upper cover, and a second jack is opened on the upper cover, and the first jack and the second jack are respectively deviated from the upper The central axis of rotation of the cover relative to the tube body. After the upper cover rotates relative to the tube body, the first insertion hole and the second insertion hole can be aligned or staggered along the axial direction of the upper tube region.

The embodiment of the utility model is further configured as: the upper cover is threadedly connected with the pipe body.

The embodiment of the utility model is further configured as follows: the fixed cover at the end of the lower tube area far away from the upper tube area is connected with a bottom cover, and the push rod is threadedly connected with the bottom cover.

The embodiment of the utility model is further set as: the inner diameter of the extraction area is smaller than the diameters of the upper pipe area and the lower pipe area.

The embodiment of the utility model is further set as: the volume of the upper pipe area is equal to the volume of the lower pipe area.

The embodiment of the utility model is further set as: the tube body is transparent.

The embodiment of the utility model is further set as: the air hole is located at one end of the extraction area close to the lower tube area;

The embodiment of the utility model is further set as: the sealing plug at the end of the upper tube area away from the lower tube area is connected with a rubber plug.

The embodiment of the utility model is further configured as: a rubber plug is sealed and fixedly connected in the first socket.

The advantages of the embodiment of the utility model are: when working, the air hole is stuck open, the needle of the syringe containing blood is inserted into the tube body from the first jack, and the blood is injected into the tube body. Blood is injected into the tube body until the blood is filled with the extraction area and the upper tube area, the needle of the syringe is pulled out from the tube body, and the upper cover is rotated so that the first jack and the second jack are staggered from each other, blocking the inner space of the tube body and the outer space. connected. Load the tube body into a centrifuge for centrifugation, the distance between the upper tube area and the central axis of rotation during centrifugation is smaller than the distance between the lower tube area and the central axis of rotation, the density of plasma is less than that of blood cells, and the density of mononuclear cells is between Between the plasma and red blood cells, the blood is divided into three layers after centrifugation. The plasma is located in the upper tube area, the red blood cells are located in the lower tube area, the mononuclear cells are located between the plasma and red blood cells, and some mononuclear cells are also located in the lower tube area. Take the tube body out of the centrifuge, rotate the push rod in the direction of screwing in, the piston pushes the mononuclear cells to gradually gather to the extraction area, and extend the needle of the syringe through the first jack and the second jack into the extraction area , collect the mononuclear cells by aspirating them into a syringe. During the separation and collection process, the exposure of blood to the air can be reduced, and the contamination of mononuclear cells by the air can be reduced. During the process of extracting mononuclear cells with a syringe, the needle extends to the bottom of the extraction area. During the extraction process, a negative pressure is generated inside the extraction area, and the plasma gradually moves toward the direction of entering the extraction area under the action of its own gravity and the negative pressure of the extraction area. In the extraction area, the collection of mononuclear cells is more thorough and the extraction efficiency is improved.

Description of drawings

In order to more clearly illustrate the implementation of the utility model or the technical solutions in the prior art, the following will briefly introduce the drawings that need to be used in the description of the implementation or the prior art. Apparently, the drawings in the following description are only exemplary, and those skilled in the art can also obtain other implementation drawings according to the provided drawings without creative work.

The structures, proportions, sizes, etc. shown in this manual are only used to cooperate with the content disclosed in the manual for the understanding and reading of those who are familiar with this technology, and are not used to limit the limited conditions that the utility model can be implemented, so there is no In technical substantive meaning, any modification of structure, change of proportional relationship or adjustment of size shall still fall within the scope of the invention disclosed in the utility model without affecting the effect and purpose of the utility model. within the scope of the technical content.

Fig. 1 is a sectional view cut along the central axis of the pipe body according to Embodiment 1 of the present utility model;

Fig. 2 is a schematic diagram of the connection structure between the rubber plug and the pipe body in embodiment 2 of the present invention;

Fig. 3 is a structural schematic diagram of Embodiment 3 of the present utility model.

In the picture:

1. Tube body; 11. Upper tube area; 12. Extraction area; 13. Lower tube area; 14. First socket; 15. Air hole; 2. Piston; 3. Push rod; 4. Top cover; 41. Second socket Hole; 5. Bottom cover; 6. Rubber plug.

Detailed ways

The implementation of the present utility model is illustrated by specific specific examples below. Those who are familiar with this technology can easily understand other advantages and effects of the present utility model from the contents disclosed in this description. Obviously, the described embodiments are the embodiment of the present utility model. Some, but not all, embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the scope of protection of the present utility model.

Example 1

A device for extracting mononuclear cells in blood, as shown in Figure 1, comprising a tube body 1, a piston 2 and a push rod 3, the push rod 3 is threadedly connected to the tube body 1, and the piston 2 is fixedly connected to the push rod 3; The pipe body 1 is divided into an upper pipe area 11, an extraction area 12 and a lower pipe area 13. The extraction area 12 is located between the upper pipe area 11 and the lower pipe area 13; The end of the push rod 3 away from the piston 2 is located outside the lower tube area 13, and when the push rod 3 rotates relative to the tube body 1, it drives the piston 2 to move axially along the lower tube area 13; the end of the upper tube area 11 away from the lower tube area 13 is provided with a first An insertion hole 14, the inner space of the upper pipe area 11 communicates with the outer space through the first insertion hole 14; an air hole 15 is opened on the extraction area 12, and the inner space of the extraction area 12 communicates with the outer space through the air hole 15.

During work, open the air hole 15 (use a plug to block when the air hole 15 is not needed), insert the syringe needle filled with blood from the first jack 14 into the tube body 1 and inject the blood into the tube body 1, and wait until the blood is full. After the tube area 13, block the air hole 15, continue to inject blood into the tube body 1 until the blood is full of the extraction area 12 and the upper tube area 11, pull out the needle of the syringe from the tube body 1, rotate the upper cover 4, and make the first jack 14 It is staggered with the second insertion hole 41 to block the communication between the internal space of the tube body 1 and the external space. Load the tube body 1 into the centrifuge for centrifugation, the distance between the upper tube area 11 and the central axis of rotation during centrifugation is smaller than the distance between the lower tube area 13 and the central axis of rotation, the density of plasma is less than that of blood cells, mononuclear cells The density is between plasma and red blood cells. After centrifugation, blood is divided into three layers. Plasma is located in the upper tube area 11, red blood cells are located in the lower tube area 13, mononuclear cells are located between plasma and red blood cells, and some mononuclear cells are also located in the lower tube area. 13 in. Take the tube body 1 out of the centrifuge, rotate the push rod 3 in the direction of screwing in, the piston pushes the mononuclear cells to gradually collect into the extraction area 12, and pass the needle of the syringe through the first socket 14 and the second socket 41 in sequence Extend into the extraction area and draw the mononuclear cells into the syringe to complete the collection. During the separation and collection process, the exposure of blood to the air can be reduced, and the contamination of mononuclear cells by the air can be reduced. During the process of extracting mononuclear cells with a syringe, the needle extends to the bottom of the extraction area 12. During the extraction process, a negative pressure is generated inside the extraction area 2, and the plasma gradually enters the extraction area 2 under the action of its own gravity and the negative pressure of the extraction area 2. Move in the direction of the extraction area, so that the mononuclear cells can be collected more thoroughly and the extraction efficiency can be improved.

The end of the upper tube area 11 away from the lower tube area 13 is rotatably connected to the upper cover 4, and the upper cover 4 is provided with a second insertion hole 41, and the first insertion hole 14 and the second insertion hole 41 are respectively deviated from the position where the upper cover 4 rotates relative to the tube body 1. On the central axis, after the upper cover 4 rotates relative to the tube body 1 , the first insertion hole 14 and the second insertion hole 41 can be aligned or staggered along the axial direction of the upper tube region 11 .

The upper cover 4 is threadedly connected with the pipe body 1 . One end of the lower tube area 13 away from the upper tube area 11 is fixedly covered with a bottom cover 5 , and the push rod 3 is screwed to the bottom cover 5 .

The inner diameter of the extraction area 12 is smaller than the diameters of the upper pipe area 11 and the lower pipe area 13 . The volume of the upper pipe area 11 is equal to that of the lower pipe area 13 . The tube body 1 is transparent. The air holes 15 are located at one end of the extraction area 12 close to the lower tube area 13 .

Example 2

A device for extracting mononuclear cells in blood, as shown in Figure 2, the difference from Embodiment 1 is that the upper cover 4 is a rubber stopper 6, and the rubber stopper 6 is sealed and connected to the upper tube area 11 away from the lower tube area One end of 13, when working, insert the needle of the syringe and pass through the rubber stopper 6 to extend into the tube body 1. During the process of inserting the syringe needle into the tube body 1 or pulling it out from the tube body 1, the rubber stopper 6 can prevent the outside air from entering inside the tube body to prevent the blood in the tube body 1 from being polluted.

Example 3

A device for extracting mononuclear cells in blood, as shown in FIG. 3 , is different from Embodiment 1 in that a rubber stopper 6 is sealed and fixedly connected to the first insertion hole 14 .

Although the utility model has been described in detail with general description and specific examples above, some modifications or improvements can be made on the basis of the utility model, which will be obvious to those skilled in the art. Therefore, the modifications or improvements made on the basis of not departing from the spirit of the utility model all belong to the protection scope of the utility model.

Claims (10)

1. A device for extracting mononuclear cells in blood is characterized in that: it comprises a tube body (1), a piston (2) and a push rod (3), and the push rod (3) is threadedly connected with the tube body (1) , the piston (2) is fixedly connected with the push rod (3); The pipe body (1) is divided into an upper pipe area (11), an extraction area (12) and a lower pipe area (13), and the extraction area (12) is located between the upper pipe area (11) and the lower pipe area (13); The piston (2) is located inside the lower tube area (13), and the piston (2) is in sealing and sliding connection with the inner wall of the lower tube area (13); The end of the push rod (3) away from the piston (2) is located outside the lower tube area (13), and when the push rod (3) rotates relative to the tube body (1), it drives the piston (2) to move axially along the lower tube area (13) ; The end of the upper pipe area (11) away from the lower pipe area (13) is provided with a first socket (14), and the inner space of the upper pipe area (11) communicates with the external space through the first socket (14); Air holes (15) are opened on the extraction area (12), and the inner space of the extraction area (12) communicates with the outer space through the air holes (15). 2. A device for extracting mononuclear cells in blood according to claim 1, characterized in that: the end of the upper tube area (11) away from the lower tube area (13) is rotatably connected with an upper cover (4 ), the upper cover (4) is provided with a second jack (41), and the first jack (14) and the second jack (41) are respectively deviated from the upper cover (4) relative to the tube body (1) The central axis of rotation, after the upper cover (4) rotates relative to the tube body (1), the first insertion hole (14) and the second insertion hole (41) can be aligned or staggered along the axial direction of the upper tube area (11). 3. A device for extracting mononuclear cells in blood according to claim 2, characterized in that: the upper cover (4) is threadedly connected with the tube body (1). 4. A kind of device for extracting mononuclear cells in blood according to claim 1, characterized in that: the end of the lower tube area (13) away from the upper tube area (11) is fixedly covered with a bottom cover ( 5), the push rod (3) is threadedly connected with the bottom cover (5). 5. A device for extracting mononuclear cells in blood according to claim 1, characterized in that: the inner diameter of the extraction area (12) is smaller than the upper tube area (11) and the lower tube area (13) )diameter of. 6. A device for extracting mononuclear cells in blood according to claim 1, characterized in that: the volume of the upper tube area (11) is equal to the volume of the lower tube area (13). 7. A device for extracting mononuclear cells in blood according to claim 1, characterized in that: the tube body (1) is transparent. 8. A device for extracting mononuclear cells in blood according to claim 1, characterized in that: the air hole (15) is located at one end of the extraction area (12) close to the lower tube area (13) . 9. A device for extracting mononuclear cells in blood according to claim 1, characterized in that: the sealing plug at one end of the upper tube area (11) away from the lower tube area (13) is connected with a rubber plug ( 6). 10. A device for extracting mononuclear cells in blood according to claim 2, characterized in that: said first insertion hole (14) is sealed and fixedly connected with a rubber stopper (6).