CN209797961U - Culture dish is collected to macrofungi hypha - Google Patents
Culture dish is collected to macrofungi hypha Download PDFInfo
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- CN209797961U CN209797961U CN201920409195.3U CN201920409195U CN209797961U CN 209797961 U CN209797961 U CN 209797961U CN 201920409195 U CN201920409195 U CN 201920409195U CN 209797961 U CN209797961 U CN 209797961U
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Abstract
The embodiment of the utility model discloses culture dish is collected to macrofungi hypha, include: the lower cover comprises a circular chassis and an annular side wall, and the side wall is provided with a seed receiving hole; the vertical distance between the upper end surface of the side wall of the lower cover and the upper surface of the chassis, namely the depth of the lower cover, is 20-30 mm; the upper cover comprises a circular cover plate and an annular outer wall, and the outer wall is provided with an inoculation through hole; the vertical distance between the lower end face of the outer wall of the upper cover and the lower surface of the cover plate, namely the depth of the upper cover, is 20-30 mm; the upper cover is covered at the opening end of the lower cover, the cover plate of the upper cover is covered at the upper end surface of the side wall of the lower cover, and a sealing cover for the inner space of the side wall of the lower cover is formed; the caliber of the outer wall of the upper cover is larger than that of the side wall of the lower cover; the depth of the lower cover is the same as that of the upper cover. The collection culture dish is convenient to inoculate, high in inoculation efficiency, low in pollution rate and high in mycelium production efficiency, and is suitable for culturing and collecting fungal hyphae.
Description
Technical Field
The utility model belongs to the technical field of the microbiological experiment, concretely relates to culture dish is collected to macrofungi hypha.
background
In order to research the genetic relationship of large fungi and utilize molecular markers to assist breeding, DNA of the large fungi is often extracted, the DNA of the large fungi can be extracted from fruiting bodies or mycelia, the purity of the DNA is influenced due to the high polysaccharide content of the fruiting bodies of the large fungi, and the purity of the DNA extracted from the mycelia is relatively high.
A typical 90mm diameter petri dish has a bottom area of 63.5cm2The amount of the liquid added at one time is generally 10ml, and the culture dish is not suitable for culturing and collecting hyphae of large fungi.
1. this dish is too shallow and has a small capacity for enriching fungal mycelia when the fungus is cultured upside down.
2. The culture of fungi is generally to take solid strains to inoculate to the center of a culture dish according to a sterile operation method, and the sterile inoculation operation of the common culture dish is not convenient and is easy to cause mixed bacteria pollution.
SUMMERY OF THE UTILITY MODEL
Therefore, the embodiment of the utility model provides a culture dish is collected to large-scale fungi hypha to solve current culture dish capacious, the inconvenient and easy contaminated problem of operation.
In order to achieve the above object, the embodiment of the present invention provides the following technical solutions:
According to a first aspect of the embodiments of the present invention, a large-scale fungi hypha collection culture dish, the culture dish includes: the lower cover is horizontally arranged and comprises a circular chassis and an annular side wall arranged on the upper surface of the chassis along the circumferential direction of the chassis, and a seed receiving hole is formed in the side wall; the vertical distance between the upper end face of the side wall of the lower cover and the upper surface of the chassis, namely the depth of the lower cover, is 20-30 mm; the upper cover is horizontally arranged and comprises a circular cover plate and an annular outer wall arranged on the lower surface of the cover plate along the circumferential direction of the cover plate, and the outer wall is provided with a seed-inoculating through hole corresponding to the seed-inoculating hole; the vertical distance between the lower end face of the outer wall of the upper cover and the lower surface of the cover plate, namely the depth of the upper cover, is 20-30 mm; the upper cover is covered at the opening end of the lower cover, the cover plate of the upper cover is covered on the upper end surface of the side wall of the lower cover, and a sealing cover for the inner space of the side wall of the lower cover is formed; the caliber of the outer wall of the upper cover is larger than that of the side wall of the lower cover; the depth of the lower cover is the same as that of the upper cover.
Further, the depth of the lower cover is 25mm, and correspondingly, the depth of the upper cover is 25 mm.
furthermore, the caliber of the side wall of the lower cover is 90mm, and the caliber of the outer wall of the upper cover is 97 mm.
Furthermore, the cover plate of the upper cover can be rotatably covered on the upper end surface of the side wall of the lower cover to form corresponding communication or intervals between the inoculation holes and the inoculation through holes.
Furthermore, the upper cover is a transparent upper cover, and the lower cover is a transparent lower cover.
Further, the internal diameter of the inoculation hole of the lower cover is 10mm, and correspondingly, the internal diameter of the inoculation through hole of the upper cover is 10 mm.
Furthermore, the bottom plate and the side wall of the lower cover are of an integrated injection molding structure.
Furthermore, the cover plate and the outer wall of the upper cover are of an integral injection molding structure.
The embodiment of the utility model provides a have following advantage:
Deepening the degree of depth of culture dish, the culture dish degree of depth that general diameter is 90mm is 15mm, and the utility model provides a collection culture dish, the degree of depth increases 10mm, solves the problem that culture dish enrichment fungal mycelium is capacious, more is favorable to the enrichment of hypha when inverting the cultivation.
The cooperation design of inoculation hole and inoculation through-hole changes the mode that the culture dish was inoculated to the fungi, need not to open the culture dish upper cover, just can utilize the inoculation to collude or the inoculation shovel inoculates through inoculation hole and inoculation through-hole, makes the inoculation more convenient, and efficiency is higher, and the pollution rate is lower.
the collection culture dish is convenient to inoculate, high in inoculation efficiency, low in pollution rate and high in mycelium production efficiency, and is suitable for culturing and collecting fungal hyphae.
Drawings
in order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It should be apparent that the drawings in the following description are merely exemplary, and that other embodiments can be derived from the drawings provided by those of ordinary skill in the art without inventive effort.
The structure, ratio, size and the like shown in the present specification are only used for matching with the content disclosed in the specification, so as to be known and read by people familiar with the technology, and are not used for limiting the limit conditions which can be implemented by the present invention, so that the present invention has no technical essential significance, and any structure modification, ratio relationship change or size adjustment should still fall within the scope which can be covered by the technical content disclosed by the present invention without affecting the efficacy and the achievable purpose of the present invention.
Fig. 1 is a schematic structural diagram of a large fungus hypha collection culture dish provided in embodiment 1 of the present invention;
Fig. 2 is a schematic structural view of a lower cover in a large-scale fungus hypha collection culture dish provided by embodiment 1 of the present invention;
Fig. 3 is a schematic structural view of an upper cover in a large-scale fungus hypha collection culture dish according to embodiment 1 of the present invention;
in the figure:
1. The lower cover 11, the chassis 12, the side wall 13 and the inoculation hole;
2. Upper cover, 21, cover plate, 22, outer wall, 23, inoculation through-hole.
Detailed Description
the present invention is described in terms of specific embodiments, and other advantages and benefits of the present invention will become apparent to those skilled in the art from the following disclosure. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
In the present invention, the terms "upper", "lower", "left", "right", "middle", and the like are used for the sake of clarity only, and are not intended to limit the scope of the present invention, and changes or adjustments of the relative relationship thereof are also considered to be the scope of the present invention without substantial changes in the technical content.
Example 1
referring to fig. 1-3, the utility model provides a culture dish is collected to macrofungi hypha, the culture dish includes: the lower cover 1 is horizontally arranged, the lower cover 1 comprises a circular chassis 11 and an annular side wall 12 arranged on the upper surface of the chassis 11 along the circumferential direction of the chassis 11, and the side wall 12 is provided with a seed receiving hole 13; the vertical distance between the upper end surface of the side wall of the lower cover 1 and the upper surface of the chassis 11, namely the depth of the lower cover 1 is 20-30 mm; the upper cover 2 is horizontally arranged, the upper cover 2 comprises a circular cover plate 21 and an annular outer wall 22 arranged on the lower surface of the cover plate 21 along the circumferential direction of the cover plate 21, and the outer wall 22 is provided with a seed through hole 23 corresponding to the seed hole 13; the vertical distance between the lower end face of the outer wall of the upper cover 2 and the lower surface of the cover plate 21, namely the depth of the upper cover 2, is 20-30 mm; the upper cover 2 is arranged at the opening end of the lower cover 1 in a covering manner, and the cover plate 21 of the upper cover 2 is arranged on the upper end surface of the side wall 12 of the lower cover 1 in a covering manner to form a sealing cover for the inner space of the side wall 12 of the lower cover 1; the caliber of the outer wall 22 of the upper cover 2 is larger than that of the side wall 12 of the lower cover 1; the depth of the lower cover 1 is the same as that of the upper cover 2.
Further, the caliber of the side wall 12 of the lower cover 1 is 90mm, and the caliber of the outer wall 22 of the upper cover 2 is 97 mm.
Further, the cover plate 21 of the upper cover 2 is rotatably covered on the upper end surface of the side wall 12 of the lower cover 1, and forms corresponding communication or interval between the inoculation hole 13 and the inoculation through hole 23.
Further, the upper cover 2 is a transparent upper cover, and the lower cover 1 is a transparent lower cover.
Further, the inner diameter of the inoculation hole 13 of the lower cover 1 is 10mm, and correspondingly, the inner diameter of the inoculation through hole 23 of the upper cover 2 is 10 mm.
Further, the bottom plate 11 and the side wall 12 of the lower cover 1 are of an integral injection molding structure.
Further, the cover plate 21 and the outer wall 22 of the upper cover 2 are of an integral injection molding structure.
The utility model provides a use method of culture dish is collected to macrofungi hypha as follows:
Respectively designing an inoculation through hole 23 and an inoculation hole 13 with the diameter of 10mm at the same position of the side surfaces of an upper cover 2 and a lower cover 1 of the large fungus hypha collection culture dish, wherein the distance between the inoculation through hole 23 and a cover plate 23 of the upper cover 2 is 3 mm;
The upper cover 2 is covered on the lower cover 1, the inoculation through hole 23 is aligned to the inoculation hole 13, when the inoculation device is used, the upper cover 2 does not need to be opened, inoculation can be carried out by utilizing an inoculation hook or a small inoculation shovel through the inoculation through hole 23 and the inoculation hole 13, after inoculation, the upper cover 2 is rotated by a certain angle, the positions of the inoculation through hole 23 and the inoculation hole 13 are staggered, and the inoculation through hole 23 of the upper cover 2 is pasted with a sealing film, so that cultivation can be carried out.
The cooperation design of inoculation hole 13 and inoculation through-hole 23 changes the mode that the culture dish was inoculated to the fungi, need not to open culture dish upper cover 2, just can utilize the inoculation to collude or small-size inoculation shovel to inoculate through inoculation hole 13 and inoculation through-hole 23, makes the inoculation more convenient, and efficiency is higher, and the pollution rate is lower.
the collection culture dish is convenient to inoculate, high in inoculation efficiency, low in pollution rate and high in mycelium production efficiency, and is suitable for culturing and collecting fungal hyphae.
Example 2
Further, the depth of the lower cover 1 is 25mm, and correspondingly, the depth of the upper cover 2 is 25 mm.
Deepening the culture dish, the culture dish depth that general diameter is 90mm is 15mm, and utility model provides a diameter that culture dish was collected to large-scale fungi hypha is 90mm, and the degree of depth is 25mm, more is favorable to the enrichment of hypha when inverting the cultivation.
the rest is the same as example 1.
although the invention has been described in detail with respect to the general description and the specific embodiments, it will be apparent to those skilled in the art that modifications and improvements can be made based on the invention. Therefore, such modifications and improvements are intended to be within the scope of the invention as claimed.
Claims (8)
1. A large fungal hyphae collection culture dish, comprising:
the lower cover (1) is horizontally arranged, the lower cover (1) comprises a circular chassis (11) and an annular side wall (12) arranged on the upper surface of the chassis (11) along the circumferential direction of the chassis (11), and the side wall (12) is provided with a seed receiving hole (13);
the vertical distance between the upper end face of the side wall of the lower cover (1) and the upper surface of the chassis (11), namely the depth of the lower cover (1), is 20-30 mm;
The upper cover (2) is horizontally arranged, the upper cover (2) comprises a circular cover plate (21) and an annular outer wall (22) arranged on the lower surface of the cover plate (21) along the circumferential direction of the cover plate (21), and the outer wall (22) is provided with a seed through hole (23) corresponding to the seed hole (13);
The vertical distance between the lower end face of the outer wall of the upper cover (2) and the lower surface of the cover plate (21), namely the depth of the upper cover (2), is 20-30 mm;
The upper cover (2) is arranged at the opening end of the lower cover (1) in a covering manner, and the cover plate (21) of the upper cover (2) is arranged on the upper end surface of the side wall (12) of the lower cover (1) in a covering manner to form a sealing cover for the inner space of the side wall (12) of the lower cover (1);
the caliber of the outer wall (22) of the upper cover (2) is larger than that of the side wall (12) of the lower cover (1);
The depth of the lower cover (1) is the same as that of the upper cover (2).
2. A large fungal hyphae collection dish according to claim 1, wherein the lower lid (1) has a depth of 25mm and correspondingly the upper lid (2) has a depth of 25 mm.
3. The large-scale fungus hypha collection petri dish according to claim 1, wherein the caliber of the side wall (12) of the lower cover (1) is 90mm, and the caliber of the outer wall (22) of the upper cover (2) is 97 mm.
4. The large-scale fungus hypha collection culture dish according to claim 1, wherein the cover plate (21) of the upper cover (2) is rotatably covered on the upper end surface of the side wall (12) of the lower cover (1) to form corresponding communication or interval between the inoculation hole (13) and the inoculation through hole (23).
5. A large-scale fungus hypha collection culture dish according to any one of claims 1-4, characterized in that the upper cover (2) is a transparent upper cover and the lower cover (1) is a transparent lower cover.
6. A large fungi hypha collection petri dish according to claim 1 or 4, wherein the inner diameter of the inoculation hole (13) of the lower cover (1) is 10mm, correspondingly, the inner diameter of the inoculation through hole (23) of the upper cover (2) is 10 mm.
7. The large-scale fungus hypha collection petri dish according to claim 1, wherein the bottom plate (11) and the side wall (12) of the lower cover (1) are of an integral injection molding structure.
8. The large-scale fungus hypha collection culture dish of claim 1, wherein the cover plate (21) and the outer wall (22) of the upper cover (2) are of an integral injection molding structure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920409195.3U CN209797961U (en) | 2019-03-28 | 2019-03-28 | Culture dish is collected to macrofungi hypha |
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| CN201920409195.3U CN209797961U (en) | 2019-03-28 | 2019-03-28 | Culture dish is collected to macrofungi hypha |
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| CN209797961U true CN209797961U (en) | 2019-12-17 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111607501A (en) * | 2020-06-05 | 2020-09-01 | 重庆工商大学 | A vegetable leaf microorganism collection device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111607501A (en) * | 2020-06-05 | 2020-09-01 | 重庆工商大学 | A vegetable leaf microorganism collection device |
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