CN209338434U - A kind of removable protein renaturation device of low temperature - Google Patents

Abstract

The utility model discloses a kind of low temperature to move protein renaturation device, including renaturation tank, cooling-water machine and moving device for objects, the renaturation tank has collet, low temperature environment needed for the cooling-water machine is connected to maintenance renaturation tank with the collet of renaturation tank by pipeline, it is characterized by: the renaturation tank and cooling-water machine are each provided on moving device for objects, the moving device for objects includes the first platform, second platform and wheel positioned at the first mesa base, the renaturation tank and cooling-water machine are arranged in the first platform, second platform is equipped with denaturation tank and constant flow pump, denaturing liquid needed for being denaturalized tank inner containment protein renaturation process, piping connection between denaturation tank and the constant flow pump, piping connection between constant flow pump and renaturation tank.Renaturation tank and cooling-water machine are arranged on moving device for objects the utility model simultaneously, sufficient it can ensure the low temperature environment needed for renaturation process and subsequent process, it is equipped with wheel on moving device for objects and helps and holds structure, keeps a whole set of renaturation device more convenient in use.

Description

A kind of removable protein renaturation device of low temperature

Technical field

The utility model belongs to field of biotechnology, specifically, being related to a kind of removable protein renaturation device of low temperature.

Background technique

Using Escherichia coli as host expresses recombinant protein, it is in inclusion bodies mostly, it is subsequent to need denaturation, renaturation etc. Series of steps can just obtain biologically active recombinant protein；The renaturation of recombinant protein generally under cryogenic have compared with Good effect, carries out in the equipment such as refrigerator, refrigerator and freezer often in concrete operations, and the confined space of these equipment just limits The scale of protein renaturation and the convenience of operation are made.

Application No. is 201720219477.8 Chinese patents to disclose a kind of dialysis machine of protein renaturation, including holds Receive the container of renaturation solution, the external container is equipped with cooling insulating layer, and cooling insulating layer is connected with cold source, the renaturation liquid level It is equipped with hanger, hanger underhung has the Dialysis container for accommodating renaturation solution, and cooling insulating layer bottom is equipped with mobile convenient for equipment Idler wheel, persistently the renaturation solution in container can be cooled down using the utility model, to guarantee dialysis renaturation temperature Degree, it is more convenient that the idler wheel of cooling insulating layer bottom convenient for equipment carries out moving operation, but in patent cold source not with container one Movement is played, the temperature of renaturation container ensures insufficient in subsequent operation, and denaturing liquid is placed in bag filter in patent, using compacted Dynamic pump will avoid excessive velocities from causing to collide between bag filter when recycling to renaturation solution.

In view of this present invention is specifically proposed.

Summary of the invention

The technical problem to be solved by the present invention is to overcome the deficiencies of the prior art and provide a kind of removable egg of low temperature White renaturation device.

In order to solve the above technical problems, the basic idea of the utility model adopting the technical scheme is:

A kind of removable protein renaturation device of low temperature, including renaturation tank, cooling-water machine and moving device for objects, the renaturation tank With collet, low temperature environment needed for the cooling-water machine is connected to maintenance renaturation tank with the collet of renaturation tank by pipeline, feature Be: the renaturation tank and cooling-water machine are each provided on moving device for objects, and the moving device for objects includes the first platform, second Platform and wheel positioned at the first mesa base, the renaturation tank and cooling-water machine are arranged in the first platform.

Further scheme as the utility model: including denaturation tank and constant flow pump, it is denaturalized tank inner containment protein renaturation Denaturing liquid needed for process, piping connection between denaturation tank and the constant flow pump, piping connection between constant flow pump and renaturation tank are described It is denaturalized tank and constant flow pump is arranged in the second platform.

Further scheme as the utility model: the denaturation pot bottom is exported equipped with denaturing liquid, with constant current pump conduit Connection.

Further scheme as the utility model: including blade, motor, motor support frame, the motor and blade electricity Connection, the motor are located in the middle part of motor support frame, and the motor support frame is located in the middle part of renaturation tank top.

Further scheme as the utility model: the motor support frame is two clamping pieces disposed in parallel, described Motor is clamped between clamping piece.

Further scheme as the utility model: the renaturation tank includes the inner casing that bottom center is equipped with drainage hole, institute State the inclined surface that the setting of inner casing bottom surface is assembled convenient for renaturation solution to drainage hole.

Further scheme as the utility model: the renaturation pot bottom is equipped with the drain pipe with check-valves, the row Liquid pipe and drainage hole piping connection.

Further scheme as the utility model: the renaturation pot bottom is equipped with support member, and the support member is tight Paste moving device for objects.

Further scheme as the utility model: the moving device for objects, which is additionally provided with, holds knot convenient for helping for pushing meanss Structure.

After adopting the above technical scheme, the utility model has the advantages that compared with prior art.

1) renaturation tank and cooling-water machine are arranged on moving device for objects simultaneously, sufficient can ensure renaturation process and after Low temperature environment needed for continuous process.

2) denaturing liquid is added dropwise into renaturation solution using constant flow pump in renaturation process, and drives blade to be stirred using motor It mixes, makes the abundant renaturation of albumen.

3) it is equipped with wheel on moving device for objects and helps and holds structure, makes a whole set of renaturation device in use more just Benefit.

Specific embodiment of the present utility model is described in further detail with reference to the accompanying drawing.

Detailed description of the invention

The a part of attached drawing as the utility model, is further understood from the utility model for providing, this is practical Novel illustrative embodiments and their description are not constituteed improper limits to the present invention for explaining the utility model. Obviously, the accompanying drawings in the following description is only some embodiments, to those skilled in the art, is not paying creation Property labour under the premise of, can also be obtained according to these attached drawings other accompanying drawings.In the accompanying drawings:

Fig. 1 is the utility model overall effect schematic diagram；

Fig. 2 is the floor map of the utility model renaturation tank；

Fig. 3 is the perspective diagram of the utility model renaturation tank；

Fig. 4 is the utility model moving device for objects structural schematic diagram；

Fig. 5 is the utility model cooling-water machine positive structure schematic；

Fig. 6 is the utility model cooling-water machine structure schematic diagram.

In figure: 1, moving device for objects；2, renaturation tank；3, constant flow pump；4, it is denaturalized tank；5, cooling-water machine；6, motor；7, collet Outlet pipe；8, collet；9, blade；10, water check valve；11, renaturation tank drain pipe；12, renaturation tank support member；13, collet is intake Pipe；14, water check valve；15, motor support frame；16, renaturation tank drainage hole；17, the second platform；18, the first platform；19, wheel； 20, it helps and holds structure；21, filler；22, water outlet；23, water return outlet.

It should be noted that these attached drawings and verbal description are not intended to the design model limiting the invention in any way It encloses, but illustrates the concept of the utility model for those skilled in the art by referring to specific embodiments.

Specific embodiment

It is practical new below in conjunction with this to keep the objectives, technical solutions, and advantages of the embodiments of the present invention clearer The technical solution in embodiment is clearly and completely described in attached drawing in type embodiment, and following embodiment is for illustrating this Utility model, but be not intended to limit the scope of the present invention.

In the description of the present invention, it should be noted that term " on ", "lower", "front", "rear", "left", "right", The orientation or positional relationship of the instructions such as "vertical", "inner", "outside" be based on the orientation or positional relationship shown in the drawings, merely to Convenient for describing the utility model and simplifying description, rather than the device or element of indication or suggestion meaning must have specific side Position is constructed and operated in a specific orientation, therefore should not be understood as limiting the present invention.

In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, term " is pacified Dress ", " connected ", " connection " shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or integrally Connection；It can be mechanical connection, be also possible to be electrically connected；It can be directly connected, it can also be indirectly connected through an intermediary. For the ordinary skill in the art, can be understood with concrete condition above-mentioned term in the present invention specifically contain Justice.

As shown in Figures 1 to 6, a kind of low temperature of the utility model move protein renaturation device include moving device for objects 1, Renaturation tank 2 and cooling-water machine 5, renaturation tank 2 and cooling-water machine 5 are arranged on moving device for objects 1.

Renaturation tank 2 has collet 8, and 8 top of collet is equipped with collet outlet pipe 7, and collet lower part is equipped with collet water inlet pipe 13, folder It covers water inlet pipe 13 and is equipped with water check valve 14, filler 21 is equipped at the top of cooling-water machine 5,5 back side of cooling-water machine is equipped with water outlet 22 and returns The mouth of a river 23 when work, is added renaturation solution in renaturation tank 2, injects pure water into cooling-water machine 5 from filler 21, connected with hose Cooling-water machine water outlet 22 and collet water inlet 13 connect cooling-water machine water return outlet 23 and collet water outlet 7 with hose, open water check valve 14, starting cooling-water machine 5 makes collet 8 full of water at low temperature and is formed into a loop, and provides protein renaturation process needs always for renaturation tank 2 Low temperature environment.

As shown in Figure 1 and Figure 4, moving device for objects 1 includes the first platform 18, the second platform 17 and wheel 19, renaturation tank 2 and cooling-water machine 5 be arranged on the first platform 18.

As shown in Figure 1 and Figure 4, a kind of low temperature of the utility model moves protein renaturation device and further includes constant flow pump 3 and hold Receive the denaturation tank 4 of denaturing liquid needed for protein renaturation process, it is second flat that moving device for objects 1 is arranged in constant flow pump 3 and denaturation tank 4 On platform 17.

When work, denaturing liquid is added into denaturation tank 4, the bottom for being denaturalized tank 4 is exported equipped with denaturing liquid, is connected and is become with hose Property liquid outlet and constant flow pump 3, constant flow pump 3 and renaturation tank 2 are connected with hose, when the renaturation solution in renaturation tank 2 is cooled to purpose temperature After degree, start constant flow pump 3, in the renaturation solution being added dropwise in renaturation tank 2 for making the slow average rate of denaturing liquid being denaturalized in tank 4.

As shown in Figures 2 and 3, a kind of low temperature of the utility model, which moves, is equipped with blade 9, motor 6 in protein renaturation device With motor support frame 15, motor support frame 15 is made of the middle top that renaturation tank 2 is arranged in two clamping pieces, and motor 6 is electric Machine support frame 15 is clamped in middle part, hangs with the blade 9 being connected electrically below motor 6.

When work, when denaturing liquid is added dropwise into renaturation tank 2 in starting constant flow pump 3, start motor 6, blade 9 is in motor 6 It is started turning under drive, is sufficiently mixed denaturing liquid and renaturation solution.

As shown in Figures 2 and 3, a kind of low temperature of the utility model moves the inner casing bottom of renaturation tank 2 in protein renaturation device Face is the inclined surface assembled to bottom center, and bottom center is equipped with drainage hole 16, convenient for waiting for that drain thoroughly empties when drain.

Lower step process operations after finishing for the ease of renaturation connect renaturation tank drain pipe 11, row at drainage hole 16 Water check valve 14 is installed in liquid pipe 11, after renaturation, which finishes, enters lower step process, water check valve 14 is opened, revives the liquid finished It can be discharged from renaturation tank 2.

As shown in Figures 2 and 3, a kind of low temperature of the utility model moves the bottom of renaturation tank 2 in protein renaturation device also Equipped with support member 12, support member 12 is close to the first plane 18 of moving device for objects 1, and package unit is prevented to be moved through It is subjected to displacement in journey, improves the safety of package unit.

It is equipped with just as shown in figure 4, a kind of low temperature of the utility model moves moving device for objects 1 in protein renaturation device Structure 20 is held in helping for pushing meanss, keeps the movement of package unit more convenient.

The above is only the preferred embodiment of the utility model, not makees in any form to the utility model Limitation, although the utility model has been disclosed with preferred embodiment as above, is not intended to limit the utility model, any to be familiar with The technical staff of this patent is not departing within the scope of technical solutions of the utility model, when the technology contents work using above-mentioned prompt A little equivalent embodiment changed or be modified to equivalent variations out, but all contents without departing from technical solutions of the utility model, Any simple modification, equivalent change and modification made by the above technical examples according to the technical essence of the present invention, still belong to In the range of the utility model.

Claims (9)

1. a kind of low temperature moves protein renaturation device, including renaturation tank, cooling-water machine and moving device for objects, the renaturation tank tool Low temperature environment needed for having collet, the cooling-water machine to be connected to maintenance renaturation tank with the collet of renaturation tank by pipeline, feature exist In: the renaturation tank and cooling-water machine are each provided on moving device for objects, and the moving device for objects includes the first platform, second flat Platform and wheel positioned at the first mesa base, the renaturation tank and cooling-water machine are arranged in the first platform.

2. a kind of low temperature according to claim 1 moves protein renaturation device, it is characterised in that: including denaturation tank and perseverance Stream pump, denaturing liquid needed for being denaturalized tank inner containment protein renaturation process, piping connection between denaturation tank and the constant flow pump, constant current Piping connection between pump and renaturation tank, the denaturation tank and constant flow pump are arranged in the second platform.

3. a kind of low temperature according to claim 2 moves protein renaturation device, it is characterised in that: the denaturation pot bottom It is exported equipped with denaturing liquid, with constant flow pump piping connection.

4. a kind of low temperature according to claim 1 moves protein renaturation device, it is characterised in that: including blade, motor, Motor support frame, the motor are electrically connected with blade, and the motor is located in the middle part of motor support frame, and the motor support frame is located at In the middle part of renaturation tank top.

5. a kind of low temperature according to claim 4 moves protein renaturation device, it is characterised in that: the motor support frame For two clamping pieces disposed in parallel, the motor is clamped between clamping piece.

6. a kind of low temperature according to claim 1 moves protein renaturation device, it is characterised in that: the renaturation tank includes Bottom center is equipped with the inner casing of drainage hole, the inclined surface that the inner casing bottom surface setting is assembled convenient for renaturation solution to drainage hole.

7. a kind of low temperature according to claim 6 moves protein renaturation device, it is characterised in that: the renaturation pot bottom Equipped with the drain pipe with check-valves, the drain pipe and drainage hole piping connection.

8. a kind of low temperature according to claim 1 moves protein renaturation device, it is characterised in that: the renaturation pot bottom Equipped with support member, the support member is close to moving device for objects.

9. a kind of low temperature according to claim 1 moves protein renaturation device, it is characterised in that: the mobile dress of the loading It sets to be additionally provided with and holds structure convenient for helping for pushing meanss.