CN208472055U - Full-automatic nucleic acid extraction amplification diagnosis all-in-one machine - Google Patents

Abstract

The utility model discloses a kind of full-automatic nucleic acid extraction amplification diagnosis all-in-one machines, include the bracket of pedestal and setting on the base, further include having: nucleic acid extraction module；Sample access mechanism；Mechanical arm mechanism；Hardware installation module；PCR module, for detecting sample, the PCR module setting is in hardware installation module side and is located at below the bracket；Biohazard Waste containing box；The top of the Biohazard Waste containing box is arranged in for storing reagent in reagent storing mechanism；Reaction cup sample introduction mechanism is arranged in the front end of the nucleic acid extraction module, and is located on the pedestal.By adopting the above technical scheme, all parts are incorporated on pedestal and form complete equipment, structure-integrated, streamlined operation, the testing time is short, and using effect is good；All circuit boards are integrated within hardware module by hardware installation module, have prevented external electromagnetic interference problem, thus prevented because electromagnetic interference and caused by data fluctuations, measuring accuracy is high.

Description

Full-automatic nucleic acid extraction amplification diagnosis all-in-one machine

Technical field

The utility model belongs to technical field of medical equipment, is one more particularly, to a kind of nucleic acid extraction and molecular diagnosis The full-automatic nucleic acid extraction amplification diagnosis all-in-one machine of body.

Background technique

When carrying out molecular diagnosis, most of molecular diagnosises are all semi-automated instruments currently on the market, need to carry out artificial It is put into the nucleic acid step extracted, quantitative analysis is being carried out by instrument, so that increasing the generated test of manual intervention Error, test speed is slow, is manually placed into the nucleic acid extracted and be easy to cause biological pollution, and examines automatically extracting amplifier molecule In disconnected equipment, instrument internal, which mutually pollutes, is the largest difficult point.

In Chinese patent literature CN104450498A, a kind of instrument for extracting nucleic acid, including shell, rack, nucleic acid are disclosed Extraction module, sample storehouse, reagent area, volley of rifle fire module and single rifle module, the rack are equipped with table top, the nucleic acid extraction mould Block, sample storehouse and reagent area are set on table top, and the volley of rifle fire module and single rifle module are located above the table top, the rack It is equipped with X arm, the X arm is equipped with volley of rifle fire Y arm and single rifle Y arm, and the volley of rifle fire Y arm and single rifle Y arm are slided along X arm, the row Rifle module is set on the volley of rifle fire Y arm, and is slided along volley of rifle fire Y arm, and the list rifle module is set on single rifle Y arm, and along list The sliding of rifle Y arm；And further disclose the nucleic acid extraction module include shell, pedestal, comb, vibrating mechanism, heating mechanism and Magnetic suck mechanism, the heating mechanism are set on the upper surface of the pedestal, and the comb is set on the vibrating mechanism, described Vibrating mechanism is located above the heating mechanism, the magnetic suck mechanism be located at the heating mechanism and the vibrating mechanism it Between, and moved along the heating mechanism upper surface, the shell is set to above the pedestal and is located at the vibrating mechanism, heating Outside mechanism and magnetic suck mechanism.

But the instrument for extracting nucleic acid in above-mentioned technical proposal is less desirable in the specific use process, and the degree of automation is not Height increases test error.

Utility model content

It does not need be manually placed into the nucleic acid extracted the technical problem to be solved by the present invention is to provide a kind of, survey Try precision height, and the full-automatic nucleic acid extraction amplification diagnosis all-in-one machine that the testing time is short.

In order to solve the above technical problems, the technical solution adopted in the utility model is, which is examined Disconnected all-in-one machine includes the bracket of pedestal and setting on the base, further includes having:

Nucleic acid extraction module is used for purifying nucleic acid, and the nucleic acid extraction module is provided with reaction platform, and reaction cup is placed In the reaction platform；

Sample access mechanism, for saving sample, the sample access mechanism is located at the side of the nucleic acid extraction module And be placed on the pedestal, the sample access mechanism is provided with sample rack；

Mechanical arm mechanism, has Y-axis horizontal box, and the vertical cabinet of Z axis does Y-axis horizontal movement along the Y-axis horizontal box； Sampling head moves up and down on the vertical cabinet of the Z axis；The Y-axis horizontal box is connected with the bracket；

Hardware installation module, for installing hardware, the hardware installation module setting the Y-axis horizontal box with it is described Bracket is connected the lower section at position；

PCR module, for detecting sample, the PCR module setting is in hardware installation module side and is located at described Below bracket；

Biohazard Waste containing box includes to be arranged in the sample access mechanism and institute for storing Biohazard Waste The containing box between PCR module is stated, and is installed on the pedestal；

The top of the Biohazard Waste containing box is arranged in for storing reagent in reagent storing mechanism；

Reaction cup sample introduction mechanism is arranged in the front end of the nucleic acid extraction module, and is located on the pedestal.

By adopting the above technical scheme, all parts are incorporated on pedestal and form complete equipment, structure-integrated, flowing water Change operation, the testing time is short, and using effect is good, and reaction cup is pushed into nucleic acid extraction module by reaction cup sample introduction mechanism, Using mechanical arm mechanism from being sampled in the reaction cup being originally added in reaction cup packaging consumptive material in sample access mechanism, reaction terminates Afterwards, nucleic acid is extracted by mechanical arm mechanism into the EP pipe of reagent storing mechanism, and reaction cup waste is focused on to medical waste In object containing box；EP pipe equipped with reagent is extracted to PCR module from reagent storing mechanism expand again by mechanical arm mechanism Detection；Integral module is designed using enclosed modulesization, has prevented equipment pollution problem caused by due to nucleic acid extraction；Using machine Tool arm mechanism transfer nucleic acid, has prevented the influence to be influenced each other because of intermodule for detection data；Hardware installation module will own Circuit board is integrated within hardware module, has prevented external electromagnetic interference problem, to prevent to make because of electromagnetic interference At data fluctuations, measuring accuracy is high.

Further improve be, the nucleic acid extraction module has a movable plate component, the movable plate component by upper moving plate with And the lower moving plate being equipped with the upper moving plate is constituted；It is provided with reaction platform and dustbin below the movable plate component, institute State the front end that reaction platform is located at the movable plate component；Driving lever component is provided with below the reaction platform, in driving lever component With the driving lever pushed reaction cup waste in the dustbin；The driving lever component also has driving lever sliding rail, the driving lever Driving lever sliding block is installed, the driving lever is arranged on driving lever sliding block on sliding rail.

After reaction, reaction cup waste is pushed in the dustbin, then Biohazard Waste is gone to by dustbin week Containing box, convenient and efficient, process is pollution-free；Driving lever sliding block allows driving lever mobile in X axis, promotes each reaction cup, After reaction, reaction cup waste is pushed in the dustbin.

Further improve is that the movable plate component further includes having upper moving plate lead screw motor and lower moving plate lead screw motor, The bar magnet installed on the upper moving plate drives magnetosheath thereon to move up and down under the driving of the upper moving plate lead screw motor, institute It states the decylization installed on lower moving plate and sloughs magnetosheath on the bar magnet under the driving of the lower moving plate lead screw motor.

Further improve is that the reaction platform includes platform framework, and the platform framework has several reactions Framework has reaction compartment between two neighboring reaction framework, and the rear and front end of the platform framework is provided with cover board.

Reaction compartment makes have certain distance positioned at the intracorporal reaction cup of reaction frame, avoids and has prevented due to nucleic acid extraction Caused by pollution problem；The rear and front end of platform framework is provided with cover board and plays work that is anti-pollution and reinforcing reaction platform With.

Further improve is that the sample access mechanism is by being located at the sample pane of top half and being located at lower half portion Sample radiator constitute；It is provided with sample cover board in the sample pane, is surrounded by by the sample pane and the sample cover board Several mutually independent sample rack accommodating cavities；The sample rack is placed in the sample rack accommodating cavity, in the sample rack The bottom of accommodating cavity is provided with sliding rail；The back side of the sample pane is provided with the sample area control circuit board with detector；It is described The cooling piece that if there is sample radiator dry plate to be spaced apart from each other.

Sample access mechanism is divide into upper part and lower part, compact-sized, lower half portion is arranged to heat spreader structures, both sufficiently Its function is played, and does not influence the work of other component such as mechanical arm mechanism；Sample rack is promoted to hold sample rack by sliding rail Chamber is set, it is convenient and efficient.

Further improve is have several test tube suit holes on the sample rack, be arranged on the sample cover board There is access port identical with test tube suit hole number on the sample rack；The sample rack bottom has to be matched with the sliding rail Sliding-rail groove；Mechanical arm mechanism grabs the sample on sample rack by access port.

Further improve is that the reagent storing mechanism and the structure of the sample access mechanism are identical.

Further improve is that the external side of Z axis vertical box of the mechanical arm mechanism is provided with sampling lead screw motor, with Setting is connected in the intracorporal sampling screw rod linkage of the Z axis vertical box, is provided with sampling head, the sampling on the sampling screw rod Head vertical cabinet of Z axis described in the driving lower edge of the sampling lead screw motor moves up and down；The outside of the Y-axis horizontal box It is provided with mobile lead screw motor, is connected with setting in the intracorporal mobile screw rod linkage of the Y-axis filter box, the Z axis vertical box Slide block set on body is on the mobile screw rod, sliding block Y-axis filter box described in the driving lower edge of mobile lead screw motor Body does Y-axis horizontal movement.

It further improves and is, the three face hollow out of Y-axis horizontal box；And the sampling head is arranged by clamping element On the sampling screw rod, the clamping element is sleeved on the sampling screw rod；By using clamping element, sampling head can be carried out Replacement adjustment guarantees that sampling is accurate.

Further improve is that the hardware installation module is set with by electro-magnetic shielding cover and heat sink, and described If being provided with dry plate hardware installation plate on heat sink, there is nylon column on the hardware installation plate；There is heat dissipation on the heat sink Hole.

Electro-magnetic shielding cover can fall most electromagnetic shielding, and all circuit boards are integrated within hardware module, are shut out External electromagnetic interference problem absolutely, thus prevented because electromagnetic interference and caused by data fluctuations.

Further improve is that the PCR module has cover board, is provided with several reagent wells on the cover board；Institute It states and is provided with hot seat corresponding with the reagent wells below cover board, heating sheet and expansion are installed in the bottom of the hot seat Increase radiator；The heating sheet is between the hot seat and the amplification radiator, between amplification radiator and the cover board It is connected with nylon cylinder；The hot seated connection enters to have optical fiber.

The PCR module of above structure, can accomplish can sample i.e. checking functions, hot seated connection enters to have optical fiber, uses optical fiber Original very large PCR equipment is contracted among slight PCR module, while also accomplishing that each sample is individually examined by conduction technique It tests, does not interfere with each other；Heating sheet is installed in the bottom of hot seat and amplification radiator, heating sheet are located at the hot seat and the expansion Increase between radiator, is more easier to control temperature；Each hot seat has corresponding reagent wells, can be individually in reagent wells Nucleic acid heated, the hot each temperature of seat is not necessarily identical, can individually control, and the mutually not shadow between adjacent reagent wells It rings, individually the temperature of hot seat every bit is identical, and temperature is uniformly distributed, and detection effect is more preferably；It is independent to solve integrated function The problem that heating amplification is carried out to each reagent, improves the test speed of all-in-one machine.

Further improve is, is provided with conducting wire in the heating sheet；The hot seat is made of hollow circuit cylinder hole, in institute State hot seat middle position it is laterally projecting have insertion hole, the optical fiber is accessed in the hot seat by the insertion hole, by leading Line heating, can be contacted multiple heating sheets with conducting wire, and heating method is more convenient；Hot seat is made of hollow circuit cylinder hole, and EP pipe can It is put by reagent wells in corresponding hot seat；16 reagent wells are provided on the cover board, corresponding, the hot seat also has 16 It is a；Step is provided in the reagent wells, multiple reagent wells, amount detection is more, and step is provided in reagent wells, and detection is facilitated to grasp Make；The amplification radiator includes the upper surface for installing the heating sheet；Two opposite sides of the upper surface vertically to Under be extended with the first cooling fin and the second cooling fin；It is additionally provided in several between first cooling fin and the second cooling fin Portion's cooling fin has heat dissipation gap between two neighboring inner fin, by the frame mode of cooling fin, so that amplification heat dissipation Device heat dissipation is more convenient, and the temperature for controlling hot seat is easier to realize, and structure is simple, at low cost；Amplification radiator passes through upper End face is connect with heating sheet, is radiated more direct；The reagent wells are evenly distributed on the cover board；Be provided on the cover board with Nylon cylinder mounting hole in mating connection, is connected using nylon cylinder, plays heat-blocking action；Reagent wells are uniformly distributed phase Mutually independent, hot seat corresponding with reagent wells is also uniformly distributed；The bottom of the hot seat also has a mounting plate, the mounting plate with The heating sheet is directly connected to, and the effect of mounting plate is arranged so that hot seat is uniformly heated, temperature control is more stable；It is described Heating sheet is fixed between hot seat and amplification radiator and has thermal insulation board cladding, prevents heat loss；The amplification radiator is used The nylon cylinder is mounted on each hot seat lower part, will not influence the temperature of hot seat in this way.

Further improve is that the Biohazard Waste containing box further includes having reaction cup waste conveyer, described There is storage mouth below the end of reaction cup waste conveyer, the storage mouth setting is covered in the Drawer-and-shell type box；Described The side of reaction cup waste conveyer and be located at the Drawer-and-shell type box cover be provided with substitute head waste disconnecting means；It is described Biohazard Waste containing box further includes having containing box guide rail, and containing box is arranged in the containing box box cover, and the containing box is logical It crosses containing box guide-track groove arranged at the bottom and the containing box guide rail is equipped, containing box guide rail is fixedly mounted on pedestal；Institute It states and is additionally provided with handle on the outside of containing box.

Further improve is that the reaction cup waste conveyer is given up by reaction cup waste conveyer belt and reaction cup Gurry transport motor is constituted；The tail end of the reaction cup waste conveyer belt is located at the top of the storage mouth；The substitute head Waste disconnecting means is located at the side of the reaction cup waste conveyer belt；The reaction cup waste conveyor width is 5 ~10cm；The reaction cup waste transport motor is located at the lower section of the reaction cup waste conveyer belt, and the reaction cup Waste transport motor is located at the Drawer-and-shell type box outer side of lid, and reaction cup waste transport motor is located at the reaction cup and is discarded The lower section of object conveyer belt, optimizes in arrangement, and area occupied is smaller, and operator is facilitated to operate, and structure is more compact；Institute Stating substitute head waste disconnecting means has hollow unloading column, and unloading column upper end is provided with end cap, on the end cap It is provided with unloading mouth；The bottom of the unloading column is connected with the substitute head waste that the Drawer-and-shell type box covers is arranged in falling into mouth It connects, hollow unloading column therefrom slides convenient for substitute head to the containing box in containing box box cover；The containing box guide rail is by installing Item and the guide rail bar being arranged on the mounting bar are constituted, and the cross section of the guide rail bar is square；The containing box guide rail The bottom of the containing box is arranged in slot, is mutually equipped with the guide rail bar, and guide rail bar is embedded in guide-track groove, ensure that containing box The stability of extraction and push-in, such structure setting also facilitate operator's operation, in narrow operating space more It is convenient to add.

Further improve is that the reaction cup sample introduction mechanism has reaction cup box, and the reaction cup box passes through sample introduction Bracket is mounted on the base；Reaction cup is assemblied in reaction cup packaging consumptive material, is pushed into the reaction cup box；The reaction One end of cup sample introduction mechanism is additionally provided with sample introduction motor, and the sample introduction motor has synchronous pulley, in the reaction cup sample introduction machine The other end of structure is additionally provided with the idle pulley matched with the synchronous pulley；It is wrapped on the synchronous pulley and the idle pulley Synchronous belt, that section being located above be placed in the reaction cup box, that underlying section is placed in the reaction cup box Outside；Reaction cup push plate is additionally provided on the synchronous belt.

The reaction cup sample introduction mechanism of above structure, distributes reaction cup box along the longitudinal, so that reaction cup sample introduction mechanism is whole Structure is limited to by reaction cup box elongated shape, and compact-sized, area occupied is small；Reaction cup is assemblied in reaction cup packaging consumptive material, It is pushed into the reaction cup box, it is therefore prevented that pollution；That section that synchronous belt is located above is placed in the reaction cup box, under being located at That section of side is placed in the outside of the reaction cup box, in this way, being pushed into reaction cup push plate by reaction cup packaging consumptive material In the reaction cup box；The reaction cup sample introduction mechanism of above structure, high degree of automation extract amplifier molecule diagnosis with full-automatic Device is equipped, and work efficiency is high.

Further improve is that the sample introduction bracket is made of four cylinders, and four cylinders are mounted on reaction cup The bottom of box；Reaction cup packaging consumption material box is additionally provided in the reaction cup box, the reaction cup packaging consumptive material is placed on institute It states in reaction cup packaging consumption material box, setting reaction cup packs consumption material box, so that consumptive material does not contact directly with reaction cup box, it is synchronous Band is not direct to contact with reaction cup packaging consumptive material between reaction cup packaging consumption material box and the bottom interior wall of reaction cup box, shuts out Pollution problem absolutely；Reaction cup packs consumptive material push-in and movement is more regular；In one end of the reaction cup box, and it is located at institute It states and is provided with a notch above sample introduction motor；The other end of reaction cup box of the notch is provided with as input end；It is provided with It can be used for adding optoelectronic switch at the position of notch, can detecte whether reaction cup packaging consumptive material is put into position；The reaction cup packet The end of dress consumption material box is provided with leader, facilitates operation, and reaction cup packaging consumption material box is hauled out；The reaction cup box it is transversal Face is rectangle；It is opening above the reaction cup packaging consumption material box；The sample introduction motor is stepper motor.

Detailed description of the invention

It is further described with reference to the accompanying drawing with the embodiments of the present invention:

Fig. 1 is the full-automatic nucleic acid extraction amplification diagnosis all-in-one machine structure schematic diagram of the utility model；

Fig. 2 is the nucleic acid extraction modular structure schematic diagram in Fig. 1；

Fig. 3 is the sample access mechanism structural schematic diagram in Fig. 1；

Fig. 4 is the mechanical arm mechanism structural schematic diagram in Fig. 1；

Fig. 5 is the hardware installation modular structure schematic diagram in Fig. 1；

Fig. 6 is the PCR modular structure schematic diagram in Fig. 1；

Fig. 7 is the Biohazard Waste housing box structure schematic diagram in Fig. 1；

Fig. 8 is the reagent storing mechanism structural schematic diagram in Fig. 1；

Fig. 9 is the reaction cup sample introduction mechanism structural schematic diagram in Fig. 1；

Figure 10 is the decomposition texture schematic diagram of Fig. 2 amplifying nucleic acid extraction module；

Figure 11 is the structural schematic diagram of reaction cup；

Figure 12 is the structural schematic diagram of the middle and lower part Fig. 2 lever body；

Figure 13 is the structural schematic diagram of reaction platform in Fig. 2；

Figure 14 is the elevating mechanism structural schematic diagram that upper moving plate and lower moving plate movement are driven in Fig. 2；

Figure 15 is PCR decomposition module structure schematic diagram in Fig. 6；

Figure 16 is the part-structure decomposition diagram in Figure 15；

Figure 17 is the reaction cup sample introduction mechanism decomposition texture schematic diagram in Fig. 9；

Figure 18 is the Biohazard Waste containing box decomposition texture schematic diagram in Fig. 7；

Wherein: 1- nucleic acid extraction module；101- upper moving plate；102- lower moving plate；103- upper moving plate lead screw motor；104- rubbish Case；105- bar magnet；106- decylization；107- driving lever；108- reaction platform；109- movable plate component；110- driving lever component；111- driving lever Sliding rail；112- driving lever sliding block；113- lower moving plate lead screw motor；114- magnetosheath；115- platform framework；116- reacts framework；117- Reaction compartment；118- cover board；119- sliding rail motor；120- slide motor；121- reacts framework slot；The oblique notch of 122-；123- tin Foil paper；124- pre-installs magnetosheath；125- driving lever rack gear；126- driving lever gear；127- driving lever motor；128- detector；129- heating Film；130- pricker；131- suction spindle；132- guide post；2- sample access mechanism；The sample area 201- control circuit board；202- sample lid Plate；203- sample rack；204- sample pane；205- sample radiator；206- sliding rail；207- sample rack accommodating cavity；208- cooling piece； 209- test tube suit hole；210- access port；211- sliding-rail groove；3- mechanical arm mechanism；301-Y axis horizontal box；302- sliding block； 303- samples lead screw motor；304- moves screw rod；305- moves lead screw motor；The vertical cabinet of 306-Z axis；307- sampling head； 308- samples screw rod 309- clamping element；4- hardware installation module；401- electro-magnetic shielding cover；402- hardware installation plate；403- nylon Column；404- heat sink；405- heat release hole；5-PCR module；501- cover board；502- heat seat；503- heating sheet；504 optical fiber；505- Expand radiator；506- reagent wells；507- nylon cylinder；508- conducting wire；509- is inserted into hole；510- step；The upper surface 511-； The first cooling fin of 512-；The second cooling fin of 513-；514- inner fin；515- heat dissipation gap；516- mounting hole；517- installation Plate；6- Biohazard Waste containing box；601- reaction cup waste conveyer；602- substitute head waste disconnecting means；603- is received Na Xianghegai；604- handle；605- containing box guide rail；606- stores mouth；607- containing box guide-track groove；608- containing box；609- Guide rail bar；610- reaction cup waste conveyer belt；611- reaction cup waste transport motor；612- unloading column；613- end cap； 614- unloading mouth；615- substitute head waste falls into mouth；616- mounting bar；7- reagent storing mechanism；8- reaction cup sample introduction mechanism； 801- reaction cup；802- reaction cup box；803- reaction cup packs consumptive material；804- idle pulley；805- reaction cup push plate；806- is synchronous Band；807- synchronous pulley；808- sample introduction motor；809- sample introduction bracket；810- cylinder；811- reaction cup packs consumption material box；812- Notch；813- handle；9- pedestal；10- bracket.

Specific embodiment

As shown in Figure 1, be the utility model full-automatic nucleic acid extraction amplification diagnosis all-in-one machine, include pedestal 9 and Bracket 10 on the pedestal 9 is set, further includes having:

Nucleic acid extraction module 1 is used for purifying nucleic acid, and the nucleic acid extraction module 1 is provided with reaction platform 108, reaction Cup 801 is placed in the reaction platform 108；

Sample access mechanism 2, for saving sample, the sample access mechanism 2 is located at the side of the nucleic acid extraction module 1 It face and is placed on the pedestal 9, the sample access mechanism 2 is provided with sample rack 203；

Mechanical arm mechanism 3, has Y-axis horizontal box 301, and the vertical cabinet 306 of Z axis is Y along the Y-axis horizontal box 301 Axis horizontal movement；Sampling head moves up and down on the vertical cabinet 306 of the Z axis；The Y-axis horizontal box 301 and the branch Frame 10 is connected；

Hardware installation module 4, for installing hardware device and/or other affiliated facilities, the hardware installation module 4 is set Set in the Y-axis horizontal box 301 lower section at the position that is connected with the bracket 10；

PCR module 5, for detecting sample, the setting of PCR module 5 is in 4 side of hardware installation module and is located at institute State 10 lower section of bracket；

Biohazard Waste containing box 6 includes to be arranged in 2 He of sample access mechanism for storing Biohazard Waste Containing box box cover 603 between the PCR module 5, and be installed on the pedestal 9；

The top of the Biohazard Waste containing box 6 is arranged in for storing reagent in reagent storing mechanism 7；

Reaction cup sample introduction mechanism 8 is arranged in the front end of the nucleic acid extraction module 1, and is located on the pedestal 9.

As shown in Fig. 2, nucleic acid extraction module 1 has a movable plate component 109, the movable plate component 109 by upper moving plate 101 and It is constituted with the lower moving plate 102 that the upper moving plate 101 is equipped with；The lower section of the movable plate component 109 is provided with 108 He of reaction platform Dustbin 104, the reaction platform 108 are located at the front end of the movable plate component 109；The lower section of the reaction platform 108 is arranged There is driving lever component 110, there is the driving lever 107 pushed reaction cup waste in the dustbin 104 in driving lever component 110；Institute Stating driving lever component 110 also has driving lever sliding rail 111, is equipped with driving lever sliding block 112, the driving lever 107 on the driving lever sliding rail 111 It is arranged on driving lever sliding block 112；Dustbin 104 is mounted in 108 side of reaction platform, collects used reaction cup 801, rubbish Rubbish case 104 is equipped with landslide, can be slid into dustbin 104 with reaction cup 801, reduce space.

Movable plate component 109 further includes having upper moving plate lead screw motor 103 and lower moving plate lead screw motor 113, the upper moving plate 101 The bar magnet 105 of upper installation drives magnetosheath 114 thereon to move up and down under the driving of the upper moving plate lead screw motor 103, institute It states the decylization 106 installed on lower moving plate 102 and sloughs magnetic on the bar magnet 105 under the driving of the movable plate lead screw motor 113 Set 114；Can be with such, movable plate component is by several mutually independent upper moving plates and several mutually independent lower moving plates Constitute, a upper moving plate corresponds to each other with a lower moving plate, compounding practice, ensure that the independence of nucleic acid extraction operation with it is efficient Property；Reaction platform 108 includes platform framework 115, and the platform framework 115 has several to react framework 116, two neighboring anti- Answering has reaction compartment 117 between framework 116, the rear and front end of the platform framework 115 is provided with cover board 118.

It as shown in Figure 10, is the decomposition texture schematic diagram of nucleic acid extraction module 1, the side setting of the driving lever sliding rail 111 There is lead screw motor 119, the lead screw motor 119 drives the transverse movement on the driving lever sliding rail 111 of driving lever sliding block 112, described It is provided with slide motor 120 on driving lever sliding block 112, gear is housed on slide motor 120, driving lever 107 is packed on rack gear, sliding block Motor 120 drives driving lever to do longitudinal movement by rack-and-pinion, and slide motor 120 drives the driving lever 107 to discard reaction cup Object pushes in the dustbin 104, corresponding, is provided with reaction framework slot 121 in the bottom of reaction framework 116, driving lever 107 can It is moved in reaction framework slot 121；The movable plate component 109 includes 5 upper moving plates and 5 lower moving plates, the upper moving plate with Lower moving plate corresponds；Each upper moving plate and lower moving plate are each independently of the others, by its movement of respective motor driven；The rubbish The front end of rubbish case 104 also sets up an oblique notch 122.

As shown in figure 11, reaction cup 801 is equipped with magnetosheath and reagent required for nucleic acid extraction, then has 123 envelope of masking foil It deposits；Magnetosheath 124 (being magnetosheath 114) is pre-installed in advance in reaction cup 801, the last one hole is to handle used magnetic in Figure 11 Set, aperture are pin hole position, other holes are reagent wells, and reaction cup surface is affixed on masking foil 123 for sealing, and down gap is can Driving lever 107 is allowed to stir the design of reaction cup 801.

As shown in figure 12, it is the structural schematic diagram of the middle and lower part Fig. 2 lever body, reacts flat for reaction cup 801 to be dragged on In platform slot (i.e. reaction compartment 117), then stirs reaction cup 801 and make the hole in reaction cup 801 with the elevating mechanism in Figure 14 On bar magnet 105 correspond, complete nucleic acid extraction；By slide motor 120 (lead screw motor) adapted driving lever sliding rail 111, driving lever Sliding block 112 controls the transverse movement of driving lever 107, then has driving lever motor 127 (stepper motor) to drive driving lever rack gear 125, driving lever tooth Wheel 126 controls the longitudinal movement of driving lever 107, and driving lever 107 is mounted in the front end of driving lever rack gear 125, can stir each anti- Cup 801 is answered to carry out nucleic acid extraction；The position of the installation of detector 128 is also shown in figure.

Furthermore it as shown in figure 13, is the concrete structure schematic diagram of reaction platform 108, the reaction platform 108 in the present embodiment If there are five station individually nucleic acid extraction can be carried out to the sample inside each reaction cup, bottom is equipped with driving lever slot and moves back bar Slot, 108 position of reaction platform corresponding to reaction cup nucleic acid extraction hole equipped with heating film 129 in order to which heating nucleic acid extracts reagent, Increase nucleic acid extraction efficiency；Reaction platform 108 is equipped with fluting below reaction platform 108, so that driving lever for being put into reaction cup 107 can stir reaction cup 801, can also heat to the corresponding hole location of reaction cup 801.

Figure 14 is the elevating mechanism structural schematic diagram that upper moving plate and lower moving plate movement are driven in Fig. 2, belongs to one in Fig. 2 Point, for grabbing the magnetosheath 114 in reaction cup 801, allow magnetosheath 114 to move up and down in different hole locations, while puncturing anti- It answers the masking foil 123 on cup 801 and draws the waste liquid in reagent wells；Specifically, pricker 130, bar magnet 105, suction spindle 131 are in silk It moves up and down simultaneously under the driving of bar motor (i.e. upper moving plate lead screw motor 103, lower moving plate lead screw motor 113), bar magnet 105 blocks Firmly magnetosheath 114, pricker 130 puncture the masking foil 123 of next 506 top of reagent wells, and suction spindle 131 absorbs the waste liquid in last hole (suction spindle 131 is on upper moving plate 101)；The mechanism is moved up and down under the driving of lead screw motor by bar magnet 105, is just begun with Driving lever 107 stirs reaction cup 801 and to pre-install 124 hole of magnetosheath alignment bar magnet 105, and bar magnet 105 moves downward, and is inserted into magnetosheath 114 It is interior and block magnetosheath 114 (bar magnet 105 is equipped with card slot, and bulge loop is equipped in magnetosheath 114), lift magnetosheath 114, stirs reaction cup 801, So that next reagent wells 506 are directed at magnetosheath 114, then upper moving plate 101 is moved downward and is put into magnetosheath 114, after waiting 2min, The magnetic bead with nucleic acid can be adsorbed on magnetosheath 114 in reagent wells 506, lifted magnetosheath 114, stirred reaction cup 801 and make next examination Agent hole 506 is directed at magnetosheath 114, places into reagent wells 506 and is cleaned, repeats the step three times, Nucleic Acid Elution is the 4th In the reacting hole of secondary cleaning, by mechanical arm mechanism 3 by the EP pipe of nucleic acid extraction to reagent storing mechanism 7, finally lift magnetosheath 114, reaction cup 801 is stirred, so that the last one reacting hole is directed at magnetosheath 114, then upper moving plate 101 is moved downward and is put into Magnetosheath, decylization push down magnetosheath 114 under the driving of lead screw motor, then lift bar magnet 105, can make 105 magnetosheath 114 of bar magnet Separation, the magnetosheath 114 used enter in reaction cup 801, reaction cup 801 are stirred, so that the reaction cup 801 and magnetosheath that are finished 114 stir together in waste slot, complete nucleic acid extraction；Guide post 132 has been also shown in figure.

As shown in figure 3, sample access mechanism 2 is by being located at the sample pane 204 of top half and positioned at the sample of lower half portion Radiator 205 is constituted；It is provided with sample cover board 202 in the sample pane 204, by the sample pane 204 and the sample cover board 202 are surrounded by several mutually independent sample rack accommodating cavities 207；The sample rack 203 is placed on the sample rack accommodating cavity In 207, the bottom of the sample rack accommodating cavity 207 is provided with sliding rail 206；The back side of the sample pane 204 is provided with band inspection Survey the sample area control circuit board 201 of device；The cooling piece 208 that if there is the sample radiator 205 dry plate to be spaced apart from each other；Sample There are several test tube suit holes 209 on frame 203, be provided with and test tube sleeve on the sample rack 203 on the sample cover board 202 Fill the identical access port 210 of 209 quantity of hole；203 bottom of sample rack has the sliding-rail groove matched with the sliding rail 206 211。

In the utility model embodiment, the structure of reagent storing mechanism 7 and the sample access mechanism 2 be it is identical, As shown in Figure 8；The mechanism has reagent rack to be put into EP pipe along sliding rail propulsion reagent frame, and reagent area control circuit board is with detection Whether device, can incude has reagent entrance, and bottom is by there is cooling piece reagent can be made to be in frozen state always.

As shown in figure 4, sampling lead screw motor 303 is provided on the outside of the vertical cabinet 306 of the Z axis of the mechanical arm mechanism 3, with The linkage of sampling screw rod 308 being arranged in the vertical cabinet 306 of the Z axis is connected, and is provided with sampling head on the sampling screw rod 308 307, the sampling head 307 vertical cabinet 306 of Z axis described in the driving lower edge of the sampling lead screw motor 303 moves up and down； The outside of the Y-axis horizontal box 301 is provided with mobile lead screw motor 305, and is arranged in the Y-axis horizontal box 301 The mobile linkage of screw rod 304 is connected, and the sliding block 302 on the vertical cabinet 306 of the Z axis is sleeved on the mobile screw rod 304, described The Y-axis horizontal box 301 described in the driving lower edge of mobile lead screw motor 305 of sliding block 302 does Y-axis horizontal movement.

In the present embodiment, 301 3 face hollow out of Y-axis horizontal box；And the sampling head 307 is arranged by clamping element 309 On the sampling screw rod 308, the clamping element 309 is sleeved on the sampling screw rod 308.

As shown in figure 5, hardware installation module 4 is set with by electro-magnetic shielding cover 401 and heat sink 404, and the heat dissipation If being provided with dry plate hardware installation plate 402 on plate 404, there is nylon column 403 on the hardware installation plate 402；The heat sink There is heat release hole 405 on 404.

As shown in fig. 6, PCR module 5 has cover board 501, several reagent wells 506 are provided on the cover board 501；Described The lower section of cover board 501 is provided with hot seat 502 corresponding with the reagent wells 506, is equipped with and adds in the bottom of the hot seat 502 Backing 503 and amplification radiator 505；The heating sheet 503 between the hot seat 502 and the amplification radiator 505, It is connected between the amplification radiator 505 and cover board 501 with nylon cylinder 507；The access of hot seat 502 has optical fiber 504.

It as shown in figure 15, is 5 decomposition texture schematic diagram of PCR module, it can be seen that the structure in terms of more details, heating sheet Two conducting wires 508 are provided in 503；The hot seat 502 is made of hollow circuit cylinder hole, horizontal in the middle position of the hot seat 502 To insertion hole 509 is protruded, the optical fiber 504 is accessed in the hot seat 502 by the insertion hole 509, in the present embodiment, 16 reagent wells 506 are provided on the cover board 501, corresponding, the hot seat 502 also has 16；It is set in the reagent wells 506 It is equipped with step 510, the amplification radiator 505 includes the upper surface 511 for installing the heating sheet 503；The upper surface 511 Two opposite sides are extended with the first cooling fin 512 and the second cooling fin 513 vertically downward；First cooling fin, 512 He It is additionally provided with several inner fins 514 between second cooling fin 513, has between two neighboring inner fin 514 and dissipates Temperature gap 515, the reagent wells 506 are evenly distributed on the cover board 501；It is provided on the cover board 501 and the nylon The mounting hole 516 in mating connection of cylinder 507, the bottom of the hot seat 502 also have a mounting plate 517, the mounting plate 517 with The heating sheet 503 is directly connected to；The heating sheet 503 is fixed between hot seat 502 and amplification radiator 505 and has thermal insulation board (not shown) cladding；The amplification nylon cylinder 507 of radiator 505 is mounted on each hot 502 lower part of seat；It is visible in Figure 16 The EP pipe being located above is inserted into reagent wells 506, from Figure 16 it can also be seen that the PCR module of the application can be sectional type, Amplification radiator 505 i.e. positioned at bottom is to separate one by one, and corresponding heating sheet 503 is also to separate one by one；Reagent 506 tetrad of hole is provided separately, and mutual independence is more preferable, it is easier to carry out temperature control.

As shown in Figure 7, the Biohazard Waste containing box 6 further includes having reaction cup waste conveyer 601, described anti- Answering has storage mouth 606 below the end of glass waste conveyer 601, the storage mouth 606 is arranged in the containing box box cover 603 On；The reaction cup waste conveyer 601 side and be located on the containing box box cover 603 that be provided with substitute head discarded Object disconnecting means 602；The Biohazard Waste containing box 6 further includes having containing box guide rail 605, and containing box 608 is arranged described In containing box box cover 603, the containing box 608 passes through containing box guide-track groove 607 arranged at the bottom and the containing box guide rail 605 is equipped, and containing box guide rail 605 is fixedly mounted on pedestal 9；Handle 604 is additionally provided on the outside of the containing box.

As shown in figure 18, the reaction cup waste conveyer 601 is given up by reaction cup waste conveyer belt 610 and reaction cup Gurry transport motor 611 is constituted；The tail end of the reaction cup waste conveyer belt 610 is located at the top of the storage mouth 606；Institute State the side that substitute head waste disconnecting means 602 is located at the reaction cup waste conveyer belt 610, the reaction cup waste 610 width of conveyer belt is 8cm, may be arranged as any size in 5~10cm, the reaction cup waste transport motor 601 Positioned at the lower section of the reaction cup waste conveyer belt 610, and the reaction cup waste transport motor 601 is located at the storage 603 outside of chamber lid；The substitute head waste disconnecting means 602 has hollow unloading column 612, on the unloading column 612 End is provided with end cap 613, is provided with unloading mouth 614 on the end cap 613；The bottom of the unloading column 612 and setting are in institute It states the substitute head waste on containing box box cover 603 and falls into mouth 615 and be connected；The containing box guide rail 605 is by 616 He of mounting bar The guide rail bar 609 being arranged on the mounting bar 616 is constituted, and mounting bar 609 is fixed on pedestal 9, the cross of the guide rail bar 609 Section is square；The bottom of the containing box 608 is arranged in the containing box guide-track groove 607, matches with the guide rail bar 609 Dress.

As shown in figure 9, reaction cup sample introduction mechanism 8 has reaction cup box 802, the reaction cup box 802 passes through sample introduction bracket 809 are mounted on the pedestal 9；Reaction cup 801 is assemblied in reaction cup packaging consumptive material 803, is pushed into the reaction cup box 802 It is interior；One end of the reaction cup sample introduction mechanism 8 is additionally provided with sample introduction motor 808, and the sample introduction motor 808 has synchronous pulley 807, in the other end of the reaction cup sample introduction mechanism 8, it is additionally provided with the idle pulley 804 matched with the synchronous pulley 807；It twines The synchronous belt 806 being wound on the synchronous pulley 808 and the idle pulley 804, that section being located above are placed in the reaction cup In box 802, that underlying section is placed in the outside of the reaction cup box 802；It is additionally provided on the synchronous belt 806 anti- Glass push plate 805 is answered, it is passable as variation, it is provided with reaction cup push plate in the two sides up and down of synchronous belt, in such manner, it is possible to Save the propulsion time of reaction cup packaging consumptive material；As shown in figure 17, the sample introduction bracket 809 is made of four cylinders 810, described Four cylinders 810 are mounted on the bottom of reaction cup box 802；Reaction cup packaging consumptive material is additionally provided in the reaction cup box 802 Box 811, the reaction cup packaging consumptive material 803 is placed in the reaction cup packaging consumption material box 811, in the reaction cup box 802 One end, and be located at the sample introduction motor 808 above be provided with a notch 812；Reaction cup packs consumptive material 803 from diagram right side (803 sides in Fig. 9) are manually pushed back into, and (801 sides in Fig. 9) reaction cup 801 enters nucleic acid extraction module on the left of diagram 1；The end of the reaction cup packaging consumption material box 811 is provided with leader 813, and the cross section of the reaction cup box 802 is rectangular Shape；The top of the reaction cup packaging consumption material box 811 is opening, and the sample introduction motor 808 is stepper motor.

When specifically used, reaction cup 801 is assemblied in reaction cup packaging consumptive material 803, as shown in figure 9, sample introduction motor 808 is dynamic Make, drive synchronous pulley 807, synchronous belt 806 rotates, and the reaction cup push plate 805 on synchronous belt 806 is mobile, reaction cup packaging consumption Material 803 is manually pushed back into reaction cup box 802 in reaction cup packaging consumption material box 811；Reaction cup 801 is by reaction cup push plate 805 It is pushed into nucleic acid extraction module 1, as shown in Fig. 2, reaction cup 801 is allocated in reaction framework 116 by driving lever 107；Utilize machinery Arm mechanism 3 is originally added in reaction cup 801 from sampling in sample access mechanism 2, and mechanical arm mechanism 3 is moved along X-axis over the mount 10 It is dynamic, it can adjust X axis position；After reaction, nucleic acid extracts the driving lever 107 into reagent storing mechanism 7 by mechanical arm mechanism 3 Reaction cup waste is pushed in dustbin 104, the reaction cup waste in dustbin 104 passes through reaction cup shown in fig. 7 Waste conveyer 601 is sent in the containing box box cover in Biohazard Waste containing box 6, and there are also substitute head waste is de- on side Handler 602 drops to substitute head in containing box box cover；Mechanical arm mechanism 3 will be equipped with reagent from reagent storing mechanism 7 again EP pipe extract to PCR module 5 and carry out augmentation detection, detection system uses fiber optic conduction technology, by original very large PCR equipment It is contracted among slight PCR module, while also accomplishing that each sample is individually examined, do not interfere with each other.

The basic principles and main features and advantage of the utility model have been shown and described above.The technical staff of the industry It should be appreciated that the present utility model is not limited to the above embodiments, the above embodiments and description only describe this The principle of utility model, on the premise of not departing from the spirit and scope of the utility model, the utility model also has various change And improvement, such as some adjustment are done for the position for being mounted on the base all parts, these changes and improvements both fall within requirement In the scope of the utility model of protection.The utility model requires protection scope is by appended claims and its equivalent circle It is fixed.

Claims (13)

1. a kind of full-automatic nucleic acid extraction amplification diagnosis all-in-one machine, includes the bracket of pedestal and setting on the base, It is characterized in that, further including having:

Nucleic acid extraction module is used for purifying nucleic acid, and the nucleic acid extraction module is provided with reaction platform, and reaction cup is placed on institute It states in reaction platform；

Sample access mechanism, for saving sample, the sample access mechanism is located at the side of the nucleic acid extraction module and sets In on the pedestal, the sample access mechanism is provided with sample rack；

Mechanical arm mechanism, has Y-axis horizontal box, and the vertical cabinet of Z axis does Y-axis horizontal movement along the Y-axis horizontal box；Sampling Head moves up and down on the vertical cabinet of the Z axis；The Y-axis horizontal box is connected with the bracket；

Hardware installation module, for installing hardware, the hardware installation module is arranged in the Y-axis horizontal box and the bracket The lower section being connected at position；

PCR module, for detecting sample, the PCR module setting is in hardware installation module side and is located at the bracket Lower section；

Biohazard Waste containing box includes to be arranged in the sample access mechanism and the PCR for storing Biohazard Waste Containing box box cover between module, and be installed on the pedestal；

The top of the Biohazard Waste containing box is arranged in for storing reagent in reagent storing mechanism；

Reaction cup sample introduction mechanism is arranged in the front end of the nucleic acid extraction module, and is located on the pedestal.

2. full-automatic nucleic acid extraction amplification diagnosis all-in-one machine according to claim 1, which is characterized in that the nucleic acid extraction Module has movable plate component, and the movable plate component is made of upper moving plate and the lower moving plate being equipped with the upper moving plate；Described The reaction platform and dustbin are provided with below movable plate component, the reaction platform is located at the front end of the movable plate component； It is provided with driving lever component below the reaction platform, has in driving lever component and pushes reaction cup waste in the dustbin Driving lever；The driving lever component also has driving lever sliding rail, and driving lever sliding block is equipped on the driving lever sliding rail, and the driving lever setting exists On driving lever sliding block.

3. full-automatic nucleic acid extraction amplification diagnosis all-in-one machine according to claim 2, which is characterized in that the movable plate component It further include having upper moving plate lead screw motor and lower moving plate lead screw motor, the bar magnet installed on the upper moving plate is in the upper moving plate screw rod Magnetosheath thereon is driven to move up and down under the driving of motor, the decylization installed on the lower moving plate is in the lower moving plate screw rod electricity The magnetosheath on the bar magnet is sloughed under the driving of machine.

4. full-automatic nucleic acid extraction amplification diagnosis all-in-one machine according to claim 2, which is characterized in that the reaction platform It include platform framework, the platform framework has several to react framework, has reaction compartment between two neighboring reaction framework, institute The rear and front end for stating platform framework is provided with cover board.

5. full-automatic nucleic acid extraction amplification diagnosis all-in-one machine according to claim 1, which is characterized in that the sample access Sample radiator of the mechanism by being located at the sample pane of top half and positioned at lower half portion is constituted；Sample is provided in the sample pane This cover board is surrounded by several mutually independent sample rack accommodating cavities by the sample pane and the sample cover board；The sample Frame is placed in the sample rack accommodating cavity, and the bottom of the sample rack accommodating cavity is provided with sliding rail；The back of the sample pane Face is provided with the sample area control circuit board with detector；The cooling piece that if there is the sample radiator dry plate to be spaced apart from each other.

6. full-automatic nucleic acid extraction amplification diagnosis all-in-one machine according to claim 5, which is characterized in that on the sample rack With several test tube suit holes, it is provided with that hole number is identical deposits with test tube suit on the sample rack on the sample cover board Take mouth；The sample rack bottom has the sliding-rail groove matched with the sliding rail.

7. full-automatic nucleic acid extraction amplification diagnosis all-in-one machine according to claim 6, which is characterized in that the reagent storage Mechanism and the structure of the sample access mechanism are identical.

8. full-automatic nucleic acid extraction amplification diagnosis all-in-one machine according to claim 1, which is characterized in that the mechanical arm machine The external side of Z axis vertical box of structure is provided with sampling lead screw motor, links with setting in the intracorporal sampling screw rod of the Z axis vertical box It is connected, sampling head, sampling head Z axis described in the driving lower edge of the sampling lead screw motor is provided on the sampling screw rod Vertical cabinet moves up and down；It is provided with mobile lead screw motor on the outside of the Y-axis horizontal box, with setting in the Y-axis water The intracorporal mobile screw rod linkage of flat case is connected, and the slide block set on the vertical cabinet of the Z axis is on the mobile screw rod, the cunning Block Y-axis horizontal box described in the driving lower edge of mobile lead screw motor does Y-axis horizontal movement.

9. full-automatic nucleic acid extraction amplification diagnosis all-in-one machine according to claim 8, which is characterized in that the Y-axis is horizontal Three face hollow out of cabinet；And the sampling head is arranged on the sampling screw rod by clamping element, the clamping element is sleeved on described It samples on screw rod.

10. full-automatic nucleic acid extraction amplification diagnosis all-in-one machine according to claim 1, which is characterized in that the hardware peace Die-filling piece is set with by electro-magnetic shielding cover and heat sink, and if dry plate hardware installation plate is provided on the heat sink, it is described There is nylon column on hardware installation plate；There is heat release hole on the heat sink.

11. full-automatic nucleic acid extraction amplification diagnosis all-in-one machine according to claim 1, which is characterized in that the PCR module With cover board, several reagent wells are provided on the cover board；It is provided with below the cover board corresponding with the reagent wells Hot seat, the bottom of the hot seat be equipped with heating sheet and amplification radiator；The heating sheet is located at the hot seat and institute It states between amplification radiator, is connected between amplification radiator and the cover board with nylon cylinder；The hot seated connection enters to have optical fiber.

12. full-automatic nucleic acid extraction amplification diagnosis all-in-one machine according to claim 1, which is characterized in that the medical treatment is useless Gurry containing box further includes having reaction cup waste conveyer, has storage below the end of the reaction cup waste conveyer Mouthful, the storage mouth setting is covered in the Drawer-and-shell type box；The reaction cup waste conveyer side and be located at described Drawer-and-shell type box, which covers, is provided with substitute head waste disconnecting means；The Biohazard Waste containing box further includes having containing box to lead Rail, containing box be arranged in the containing box box cover, the containing box by containing box guide-track groove arranged at the bottom with it is described Containing box guide rail is equipped；Handle is additionally provided on the outside of the containing box.

13. full-automatic nucleic acid extraction amplification diagnosis all-in-one machine according to claim 1, which is characterized in that the reaction cup Sample introduction mechanism has reaction cup box, and the reaction cup box is mounted on the base by sample introduction bracket；Reaction cup is assemblied in instead It answers in cup packaging consumptive material, is pushed into the reaction cup box；One end of the reaction cup sample introduction mechanism is additionally provided with sample introduction motor, institute Sample introduction motor is stated to be additionally provided with and the synchronous pulley phase with synchronous pulley in the other end of the reaction cup sample introduction mechanism The idle pulley of cooperation；The synchronous belt being wrapped on the synchronous pulley and the idle pulley, that section being located above are placed in described anti- It answers in glass box, that underlying section is placed in the outside of the reaction cup box；Reaction cup is additionally provided on the synchronous belt Push plate.