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CN208318102U - Cell storage device - Google Patents

Cell storage device Download PDF

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Publication number
CN208318102U
CN208318102U CN201820993981.8U CN201820993981U CN208318102U CN 208318102 U CN208318102 U CN 208318102U CN 201820993981 U CN201820993981 U CN 201820993981U CN 208318102 U CN208318102 U CN 208318102U
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CN
China
Prior art keywords
pipette
liquid relief
storage device
liquid
lid
Prior art date
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Active
Application number
CN201820993981.8U
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Chinese (zh)
Inventor
李首
李首一
石晓川
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Jilin Sun Bird Regeneration Medical Engineering Co Ltd
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Jilin Sun Bird Regeneration Medical Engineering Co Ltd
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Publication date
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Priority to CN201820993981.8U priority Critical patent/CN208318102U/en
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Abstract

The utility model relates to cell field of storage, in particular to cell storage device.The cell storage device, including ontology and lid;The ontology is detachably connected with the lid, and the ontology is provided with liquid-transfering device, and the liquid-transfering device includes sequentially connected liquid relief mouth, pipette, liquid relief cylinder and pipette;The liquid relief cylinder is placed in the pipette;Port of the pipette far from liquid relief cylinder is round and smooth flat nick.The utility model is convenient with liquid relief, and sealing effect is good, safety, health, it is not easy to pollute.

Description

Cell storage device
Technical field
The utility model relates to cell field of storage, in particular to cell storage device.
Background technique
Culture medium refers to people's all kinds of nutriments according to needed for microbial growth, by certain concentration by artificial It is referred to as made of method mixing cooperation for a kind of nutrient matrix of microorganisms growth, culture medium is microorganism growth Material base is the important foundation work of microorganism detection work
In scientific research cell culture experiments, a small amount of culture medium need to be only taken every time to cell culture, therefore cell culture needs Packing saves.Traditional method is divided in the cell of culture in open vial, is then sealed with sealed membrane.Due to dividing The holding time is longer in air for cell after dress, and the isolation effect of open glass bottle and sealed membrane is limited, and contains in culture medium Become to have so that the cell of Preservation in sterile condition is subject to the pollution of the microorganisms such as bacterium in environment after packing compared with multi-nutrient Bacterium cell.And in pollution early stage, due to and not formed macroscopic bacterium colony or culture medium in have no apparent precipitating and Muddiness continues to use in culture so as to be mistakenly considered the culture medium still sterile by real user, causes the dirt to culture cell Dye.It there is no preferable solution in response to this at present or be to abandon cell to after culture cell contamination, it is again real It tests;It is when finding the pollution of macroscopic culture medium, pollution level is more serious, only discarding culture medium.These ways Not only delay experiment progress, but also waste reagent.Therefore needing prevents or reduce culture medium is contaminated in storage and use process A kind of device.
Utility model content
In view of this, the present invention provides a kind of cell storage devices.The cell storage device, liquid relief is convenient, sealing Effect is good, safety, health, it is not easy to pollute.
In order to realize above-mentioned purpose of utility model, the utility model the following technical schemes are provided:
The utility model provides a kind of cell storage device, including ontology 1 and lid 2;The ontology 1 and the lid 2 are detachably connected, and the ontology 1 is provided with liquid-transfering device 3, and the liquid-transfering device 3 includes sequentially connected liquid relief mouth 4, imbibition Pipe 5, liquid relief cylinder 6 and pipette 7;The liquid relief cylinder 6 is placed in the pipette 5;The end of the pipette 7 far from liquid relief cylinder 6 Mouth is round and smooth flat nick.
Preferably, it is described be detachably connected including be threadedly coupled, snap connection or hinge connection.
By using above-mentioned technical proposal, bottle cap and body are connected through a screw thread, stable connection, and sealing effect is good, are avoided The phenomenon of leakage caused by toppling over is provided with liquid-transfering device on liquid relief mouth, carries out pipetting, side without individually preparing pipette Just liquid relief, and liquid-transfering device sealing effect is good, safety, health, it is not easy to pollute.And round and smooth flat nick can not damage carefully Born of the same parents have protective effect to the cell taken is washed.
Preferably, 6 bottom of liquid relief cylinder is equipped with connector 8, the pipette 7 is detachably set to connector 8.
Preferably, being equipped with filter membrane 9 between the pipette 5 and the liquid relief cylinder 6.
By using above-mentioned technical proposal, it is equipped with connector in liquid relief cylinder bottom, pipette, liquid relief are set on connector The detachable setting of pipe, replacement and maintenance are simple, and filter membrane is equipped between the pipette 5 and the liquid relief cylinder 6, prevents from moving Bacterial adsorption is brought into liquid relief cylinder on inhaling night pipe by pipette during liquid, to further pollute the culture medium in body.
Preferably, indicating scale 10 on the liquid relief cylinder 6.By using above-mentioned technical proposal, in the side wall of liquid relief cylinder It is upper to be provided with scale, liquid relief amount can be observed at any time in liquid relief.
Preferably, the position that the ontology 1 is connect with the lid 2 is provided with leakage-proof ring 11.By using above-mentioned skill Art scheme prevents the leakage for having culture medium in pipetting processes, and the height of leakage-proof ring is gradually obliquely installed along side, and leakage is facilitated to train The recycling and outlet of base are supported, it is easy to operate.
Preferably, the lid 2 is equipped with the sealing column 12 to match with liquid relief mouth 4.
Preferably, the sealing column 12 is equipped with sealing ring 13.
Preferably, the sealing column 12 and the junction of the lid 2 are equipped with reinforcing rib 14.
Preferably, the circumferential junction for being set to the sealing column 12 and the lid 2 of reinforcing rib 14, described to add The quantity of strengthening tendons 14 is no less than one.
By using above-mentioned technical proposal, it is equipped with sealing column in lid, when closing lid, sealing column just gets lodged in pipette In the groove at top, there is conflict effect to pipette, reduce inner and outer air pressure and float up and down caused by pipette.
Preferably, the body bottom is equipped with anti-skid chequer.
By using above-mentioned technical proposal, it is provided with anti-skid chequer in body bottom, increases the frictional force of body bottom, reinforces The antiskid effect of body bottom.
Preferably, being cased with O-ring seal in the pipette, the material of the O-ring seal uses natural rubber.
By using above-mentioned technical proposal, it is provided with O-ring seal in pipette, pipette can be enhanced in liquid relief With the obturation effect between liquid relief cylinder, culture medium liquid relief effect is more preferable, safe and sanitary, and O-shaped sealing uses natural rubber material It is made, natural rubber has that preferable elasticity, stretching strength are high, tear resistance and wearability are good, be easy to and other materials bond The features such as.
The utility model provides a kind of cell storage device by adopting the above technical scheme, and compared with prior art, this is practical Novel advantage is: lid and ontology are detachably connected by screw thread etc., and stable connection, sealing effect is good, and the face of keeping away, which is toppled over, causes The phenomenon of leakage, on the body be provided with liquid-transfering device, without individually prepare pipette carry out pipetting, in liquid relief cylinder bottom Equipped with connector, pipette is set on connector, pipette is detachably arranged, and replaces and safeguards simply, in pipette and moves Fluid cylinder quality inspection is equipped with filter membrane, prevents the bacterial adsorption in pipetting processes from being brought into liquid relief cylinder on inhaling night pipe by pipette, Lid is equipped with sealing column, and when closing lid, sealing column is just got lodged in the groove at the top of pipette, has to pipette and contradicts Effect reduces inner and outer air pressure and floats up and down caused by pipette, and obturation effect is good, prevents or reduces culture medium or cell and storing up Deposit and use process in be contaminated.And round and smooth flat nick can not damaging cells, there is protective effect to the cell that takes is washed.
Detailed description of the invention
In order to illustrate the embodiment of the utility model or the technical proposal in the existing technology more clearly, below will be to embodiment Or attached drawing needed to be used in the description of the prior art is briefly described.
Fig. 1 shows the structural schematic diagram of the utility model embodiment;
Fig. 2 shows the axial sectional view of embodiment;
Fig. 3 shows the structural schematic diagram of liquid-transfering device in embodiment;
Appended drawing reference: 1, lid;2, ontology;3, liquid-transfering device;4, liquid relief mouth;5, pipette;6, liquid relief cylinder;7, liquid relief Pipe;8, connector;9, filter membrane;10, scale;11, leakage-proof ring;12, sealing column;13, sealing ring;14, reinforcing rib.
Specific embodiment
The utility model discloses a kind of cell storage device, those skilled in the art can use for reference present disclosure, suitably Improve realization of process parameters.In particular, it should be pointed out that all similar substitutions and modifications are for a person skilled in the art It will be apparent that they are considered as being included in the utility model.Connection relationship between the component and component of the utility model It is described by preferred embodiment, related personnel can obviously not depart from the content of the present invention, spirit and scope The interior connection relationship between elements illustrated herein and component is modified or appropriate changes and combinations, carrys out implementation and application sheet Utility model technology.
Traditional method is divided in culture medium in open vial, is then sealed with sealed membrane.After packing The holding time is longer in air for culture medium, and the isolation effect of open glass bottle and sealed membrane is limited, and liquid was easy every time Amount, causes to waste, and needs to be separately provided suction pipe absorption, troublesome in poeration, and suction pipe residual quantity is more, inhales abandon every time, and waste Reagent, therefore it is provided with a kind of cell storage device, built-in liquid-transfering device, liquid-transfering device obturation effect is good, prevents or reduce training Base is supported to be contaminated in storage and use process.
A kind of cell storage device is equipped with interior spiral shell in the opening of ontology 2 such as Fig. 1 and Fig. 2, including ontology 2 and lid 1 Line is equipped with a circle external screw thread in the inside of lid 1, and ontology 2 and lid 1 are connected through a screw thread.
In other technical solutions, ontology 2 is equipped with leakage-proof ring 11, and leakage-proof ring 11 is equipped with external screw thread, and 1 screw thread of lid connects It is connected on leakage-proof ring 11.
Liquid-transfering device 3 is equipped among ontology 2, liquid-transfering device 3 includes liquid relief mouth 4.
Such as Fig. 3, liquid-transfering device 3 includes being plugged in liquid relief mouth 4, pipette 5, liquid relief cylinder 6 and pipette 7,5 inner sleeve of pipette In liquid relief cylinder 6;Filter membrane 9 is equipped between pipette 5 and liquid relief cylinder 6, the barrel of liquid relief cylinder 6 is equipped with scale 10, in imbibition It is equipped with groove at the top of pipe 5, is cased with O-ring seal in pipette 5, pipette 5 slides up and down extraction culture medium along liquid relief cylinder 6.? 6 lower part of liquid relief cylinder is equipped with connector 8, and dismountable pipette 7 is equipped on connector 8.
It is equipped with aperture sealing column 12 compatible with the groove of pipette 5 among lid 1, is cased with outside sealing column 12 close Seal 13 is circumferentially enclosed in lid 1 and 12 junction of sealing column equipped with several reinforcing ribs 14.
In actual work, spiral unscrews lid 1, by 1 upside down of lid in workbench, upward sliding pipette 5, culture Base or cell suck liquid relief cylinder 6 out of ontology 1, take out liquid relief cylinder 6, by pipette 5 with respect to 6 slide downward of liquid relief cylinder by culture medium Or cell is forced into culture dish out of liquid relief cylinder 6.Liquid-transfering device 3 is put back into liquid relief mouth, the top of pipette 5 after the completion of liquid relief Portion is equipped with groove, and lid 1 is equipped with bore sealing column 12 compatible with groove, when lid 1 is screwed back on leakage-proof ring 11, Sealing column 12 is got lodged in groove, has both been played the role of sealing function or has been played conflict to pipette, sealing effect is good.In sealing column 12 It is cased with sealing ring 13, the sealing effect for improving sealing column 12 is good, circumferentially sets in the sealing column 12 between sealing column 12 and lid 1 There are several reinforcing ribs 14, enhances the fastness that upper cover is connect with sealing column 12.
Lid and ontology are detachably connected by screw thread etc., and stable connection, sealing effect is good, leakage caused by the face of keeping away is toppled over Phenomenon is provided with liquid-transfering device on the body, carries out pipetting without individually preparing pipette, is equipped with and connects in liquid relief cylinder bottom Pipette is arranged in connector on connector, and pipette is detachably arranged, and replaces and safeguards simply, in pipette and liquid relief cylinder matter Inspection is equipped with filter membrane, prevents the bacterial adsorption in pipetting processes from being brought into liquid relief cylinder on inhaling night pipe by pipette, on lid Equipped with sealing column, when closing lid, sealing column is just got lodged in the groove at the top of pipette, has conflict effect to pipette, Reduce inner and outer air pressure float up and down caused by pipette, obturation effect is good, prevent or reduce culture medium or cell store and It is contaminated in use process.And round and smooth flat nick can not damaging cells, there is protective effect to the cell that takes is washed.
The above is only the preferred embodiment of the utility model, it is noted that for the common skill of the art For art personnel, without departing from the principle of this utility model, several improvements and modifications can also be made, these improve and Retouching also should be regarded as the protection scope of the utility model.

Claims (10)

1. a kind of cell storage device, including ontology (1) and lid (2);The ontology (1) detachably connects with the lid (2) It connects, which is characterized in that the ontology (1) is provided with liquid-transfering device (3), and the liquid-transfering device (3) includes sequentially connected liquid relief Mouth (4), pipette (5), liquid relief cylinder (6) and pipette (7);The liquid relief cylinder (6) is placed in the pipette (5);The shifting Port of the liquid pipe (7) far from liquid relief cylinder (6) is round and smooth flat nick.
2. cell storage device according to claim 1, which is characterized in that it is described be detachably connected including be threadedly coupled, It snaps connection or hinge connects.
3. cell storage device according to claim 2, which is characterized in that liquid relief cylinder (6) bottom is equipped with connector (8), the pipette (7) is detachably set to connector (8).
4. cell storage device according to claim 3, which is characterized in that the pipette (5) and the liquid relief cylinder (6) Between be equipped with filter membrane (9).
5. cell storage device according to claim 4, which is characterized in that indicate scale (10) on the liquid relief cylinder (6).
6. cell storage device according to claim 5, which is characterized in that the ontology (1) connect with the lid (2) Position be provided with leakage-proof ring (11).
7. cell storage device according to claim 6, which is characterized in that the lid (2) is equipped with and liquid relief mouth (4) phase Matched sealing column (12).
8. cell storage device according to claim 7, which is characterized in that the sealing column (12) is equipped with sealing ring (13)。
9. cell storage device according to claim 8, which is characterized in that the sealing column (12) and the lid (2) Junction be equipped with reinforcing rib (14).
10. cell storage device according to claim 9, which is characterized in that the reinforcing rib (14) is circumferentially set to institute The junction of sealing column (12) Yu the lid (2) is stated, the quantity of the reinforcing rib (14) is no less than one.
CN201820993981.8U 2018-06-26 2018-06-26 Cell storage device Active CN208318102U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201820993981.8U CN208318102U (en) 2018-06-26 2018-06-26 Cell storage device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201820993981.8U CN208318102U (en) 2018-06-26 2018-06-26 Cell storage device

Publications (1)

Publication Number Publication Date
CN208318102U true CN208318102U (en) 2019-01-04

Family

ID=64785056

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201820993981.8U Active CN208318102U (en) 2018-06-26 2018-06-26 Cell storage device

Country Status (1)

Country Link
CN (1) CN208318102U (en)

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