CN208254645U - A kind of time-resolved spectrum and lifetime measurement module and device - Google Patents

Abstract

This patent discloses a kind of time-resolved spectrum and lifetime measurement module, the sample that the module is stimulated using motor driven is in Space Rotating, it is spatially separated the luminous signal of different delays time, short space is amplified by lens again, and specific spatial domain is selected to be observed, time-resolved detection may be implemented.The module and imaging sensor are used in combination, the measurement of lifetime of excited state may be implemented；The module and spectrometer are used in combination, can be used for time-resolved spectral detection.According to the revolving speed of the resolution ratio of optical imagery and existing motor, the temporal resolution of the device can achieve nanosecond, can satisfy the measurement of most of fluorescence lifetimes.The service life of the light source measurement excitation state of the stable state of each any wavelength can be used in this method, does not need expensive light-pulse generator and detector and locking phase control, has lower cost.

Description

A kind of time-resolved spectrum and lifetime measurement module and device

Technical field

The present invention relates to the manufactures of the spectrum of time-resolved luminescence generated by light and lifetime measurement method and pertinent instruments device And application.Belong to optical instrument manufacture and instrument analysis field.

Background technique

In luminescence generated by light phenomenon, molecule is illuminated by the light excitation and transits to excitation state, and the molecule of excitation state can be by discharging light Son returns to ground state, the i.e. fluorescence or phosphorescence of molecule.The excitation state of molecule will be when crossing one section there are service life, the i.e. molecule of excitation state Between after just release photon return to ground state, and the lifetime of excited state of different molecular is respectively different, and the molecular-excited state service life is longer, Lighting time interval is longer.Under normal conditions, the fluorescence lifetime of molecule is in nanosecond, phosphorescent lifetime can achieve Microsecond grade with On.Delayed fluorescence molecule, service life also can achieve Microsecond grade even Millisecond.

In the measurement of fluorescence and phosphorescence, generally require two optical paths of building: optical path is first is that excitation light path, i.e. exciting light shine The optical path for penetrating sample, the purpose is to so that the molecule in sample is illuminated by the light excitation；Optical path is second is that detection optical path, i.e. sample shine and reach The optical path of detector, the purpose is to detect the information such as sample luminous spectrum, intensity.In the measurement process of stable state, optical path one It is opened simultaneously with optical path two, i.e., exciting light measures under conditions of irradiating sample always, and this mode can obtain sample and shine light The information of spectrum and intensity, but the information in sample excitation state service life cannot be obtained.

With the reach of science, the excitation state research of molecule more comes about important in basic science and detection application.In order to The information and time-resolved spectrum, people for obtaining the molecular-excited state service life have developed Instantaneous Spectrometer.Instantaneous Spectrometer, including Transient absorption and transient state Fluorescence Spectrometer have important application in the photochemical research of optical physics.(referenced patent prospectus CN201310392018.6, CN200510092520.0, CN201180017387.6, CN201110005032.7 etc..) for reality Existing time-resolved measurement, it usually needs open detection optical path after closing excitation light path, i.e., after stopping excitation, measure sample Product shine.Based on such thinking, people have developed the method and instrument of many measurement delayed luminescences.(referenced patent discloses Specification CN201310392018.6, CN200510092520.0, CN201180017387.6, CN201110005032.7 etc..) Wherein more commonly used method is excited using light-pulse generator, and in a cycle, light application time is very short, the luminous letter of remaining time Number be delayed luminescence signal；The song changed over time using the detectable sample luminous intensity of the single photon counter of time correlation Line.Another common method is also using light-pulse generator, with high speed camera (ICCD), streak camera with gate control function Equal detection delays luminous signal.The price of these ultrafast light sources and high speed camera is very expensive (to refer to Chinese patent CN201410353200.5, CN201310694918.6, CN201310027775.3), it is difficult to popularize.

For this purpose, people have developed some detection methods to reduce the cost of time resolution detection.For example, patent US6839134B2, CN205080051U and CN106066317A disclose using a chopper and meanwhile control excitation light path and The detection of delayed luminescence also may be implemented without using laser, high speed camera, TTL control for the method for detecting optical path.

However with regard in disclosed technology, the revolving speed of existing mechanical chopper is at 200 revolutions per seconds hereinafter, although some researchs In motor speed can achieve 2000 revolutions per seconds or more, but its load is limited, limits the time resolution based on motor rotation The temporal resolution of detection method is also difficult to detect for lifetime of excited state in Microsecond grade material below.

Patent (CN201710042628.1) discloses a kind of detection method for fluid delayed luminescence, by fluid Movement realizes time-resolved detection to shift the position of sample, however the flow velocity of fluid is limited, needs very big pressure and very Thin pipeline realizes that the flow at high speed of fluid, this method are also difficult to realize the detection of microsecond delay.

Patent (CN201711110647.X) discloses a kind of method using the imaging sensor measurement service life, does not need arteries and veins Punching and locking phase control, have ultralow cost, however in existing motor technology, temporal resolution is extremely difficult to microsecond Below.

Summary of the invention

This patent is a kind of important improvement of patent (CN201711110647.X), the sample being all stimulated using motor driven This is spatially separated the luminous signal of different delays time, time-domain information is converted to spatial-domain information in Space Rotating It is detected.Theing improvement is that for this patent amplifies observation using space of the optical amplification system to tiny area, Under identical motor speed, temporal resolution can be improved tens times or more by technology disclosed in this patent, realize the grade of nanosecond Temporal resolution.

To reach above-mentioned target, this patent discloses a kind of measurement module, which can visit in conjunction with imaging sensor, photoelectricity It surveys the existing optical gauge such as device, spectrometer to use, is used for time-resolved spectral detection and life tests.

The module includes motor, turntable, object lens, dichroscope, pin hole, light source；

The light that wherein light source projects is reflected by dichroscope, is focused on turntable using object lens through needle passing hole；

Rotation is driven by motor in turntable；

Sample to be tested is placed on turntable, and the fluorescence or phosphorescence issued, through dichroscope, can be visited after object lens It surveys.

A kind of preferred measurement module, it is characterised in that: light source is selected from collimated light source.

A kind of preferred measurement module, it is characterised in that: object lens are selected from micro objective.

A kind of preferred measurement module, it is characterised in that: turntable is by motor shaft even driving rotation.

Another preferred measurement module, it is characterised in that: turntable and gear shaft connect, motor and another gear shaft Even, and the gear on motor is engaged with the gear on turntable.The advantage of this scheme is, before motor load is sufficiently large It puts, can be engaged by the gear of the different numbers of teeth, to obtain higher rotary speed.

The method that above-mentioned module is used for lifetime measurement are as follows: shone and focused on using the sample that lens will transmit through dichroscope On imaging sensor, the luminous picture of shooting sample, the photo to shine to sample is analyzed, and it is bent to establish the decaying to shine at any time Line, fitting can obtain the service life.

The specific same patent of principle (CN201711110647.X) is consistent:

Exciting light focuses on turntable a bit, which is excitation point, when sample at that point when, can be excited,

Again due to the rotation of turntable, so that sample leaves excitation point, and continuous illumination after being excited,

It is rotated by turntable, makes space release of the delayed luminescence of sample except excitation point,

With the rotation of turntable, the luminous track camber of sample,

By object lens, the luminous track of the arc of sample is imaged on imaging sensor, it can obtain the track that shines Photo；

In the photo, the gray value of the luminous intensity of certain point and the point is positively correlated, and delay time and point distance swash The arc length for sending out point is related, and motor is uniform rotation, thus, it is known that the radius of turn of motor speed and sample, it can calculate The delay time of each luminous point out.So as to establish the attenuation curve to shine at any time, and then it is fitted and obtains the service life.

In order to determine that motor speed, this patent disclose another preferred measurement module in real time, it is characterised in that:

In front in disclosed module basis, there is light hole on the turntable in module,

In addition, module further includes a miniature light sources and optical detector,

Miniature light sources are selected from LED or laser light source,

Optical detector is selected from photodiode, phototriode, avalanche diode；

Also, the positional relationship of above-mentioned miniature light sources and detector and turntable are as follows: the light that light source issues can be by turning The light hole of disk enters optical detector, i.e. detector is used to detect the light by light hole.

The principle of module measurement motor speed are as follows: if the illumination of miniature light sources is mapped on optical detector, will make to visit It surveys device and generates photoelectric current, however optical path is separated by turntable, only light hole is gone among exciting light and detector, can just make to excite Light passes through；With the rotation of motor, light hole can periodically go to the centre of exciting light and detector, thus detector generates Photoelectric current is also periodic, and its frequency is directly proportional to motor speed, thus passes through timing counter or frequency meter, shows The frequency that the equipment such as wave device measure the photoelectric current can know the revolving speed of motor.

According to principle discussed earlier, this patent discloses a kind of lifetime measurement device, which is characterized in that the mould including front Block, and including imaging sensor and corresponding lens；Also, sample it is luminous by object lens, through dichroscope, by lens It focuses in imaging sensor.

From the principle of measurement it is found that this method is substantially that the decay of luminescence information of time domain is converted to shining for spatial domain Strength information, arc length is directly proportional to delay time, thus, temporal resolution as high as possible is obtained, must be improved as far as possible The movement velocity of sample and the spatial resolution of imaging.In above-mentioned module, object lens have focusing light effect, thus can be by image Amplification, in addition, the lens of imaging sensor can also be such that image is further amplified.This optical amplifier being made of 2 lens System is widely used in microscope, and image can be made to amplify several hundred to thousands of times.Image by amplification has higher sky Between resolution ratio, the raising of spatial resolution directly enhances temporal resolution, relative to the measuring device of not object lens, time point Resolution can be improved hundreds and thousands of times.Theoretically, the temporal resolution of this method can achieve the space point of optical microscopy The resolution limit.

The spatial resolution of optical microscopy imaging decides the minimum range d that can distinguish two luminous points, the distance It may be considered the diffraction limit of optical imagery.

Due to delay time to apart from directly proportional, thus the calculation formula of temporal resolution are as follows: d/v；Wherein v is sample Movement velocity.

Assuming that rotary speed is k, the radius of turn of sample is r, according to circumference calculation formula, the movement velocity v of sample= 2 π kr,

The thus calculation formula of temporal resolution are as follows: d/ (2 π kr).

If rotary speed is 500 revolutions per seconds, radius of turn is the 4/ π nm of cm, d=400, then temporal resolution is 10ns, That is the light intensity variation that 10ns occurs can be recorded by imaging sensor, it is sufficient to for measuring hair of the service life more than several nanoseconds Light.Such time precision is considerably beyond the attainable temporal resolution of general mechanical chopper institute, more even more than The time precision of photodiode and high speed camera.If being more than 2000 revolutions per seconds of motor using revolving speed, radius of turn is more than 10/ π cm, then temporal resolution can achieve 1 ns, realizes the lifetime measurement of subnanosecond grade, can measure the most of fluorescence longevity Life.

Relative to the method that single photon counter, streak camera survey the service life, the above method does not need high speed detector not only, It is not necessary to ultrafast light source, significantly reduces cost.

Since excitation state at any time decay by exponentially, thus, the non-delayed luminous intensity of sample may be much higher than delay It shines, this will cause, and shining for excitation point is too strong, and may interfere with the luminous detection of delay.In order to reduce the non-delayed hair of sample The interference of light adjusts the position of light source and pin hole, or adjust the reflection angle of dichroscope in above-mentioned apparatus, or The position for adjusting imaging sensor focuses on exciting light except the visual field to adjust field range, and makes the track of delayed luminescence It falls in field range, the non-delayed luminous signal of exciting light scattering and sample can be significantly reduced in this way, improve detection letter It makes an uproar ratio.

According to the working principle of above-mentioned module, the sample that motor driven is stimulated is in space high-speed rotation, so that not co-extensive The radiative relaxation optical signal of slow time is spatially separated, thus selects the optical signal measured in some spatial domain Realize time-resolved spectral measurement.Method particularly includes: selectivity collects sample a certain section of the optical signal in track that shines It is that selectivity measures the luminous signal of sample some time domain into spectrometer；The optical signal of this section of luminous track This signal light can be collected into spectrometer, it can measure sample by a part successively through object lens, the dichroscope in module This luminescent spectrum.

According to this method, this patent discloses a kind of spectral measurement device, which is characterized in that the module including front, and Including diaphragm and spectrometer；

Wherein it is luminous to be used to detect the sample through dichroscope for spectrometer, i.e. the light inlet of spectrometer is against dichroscope With the rear of object lens；Diaphragm is located between the dichroscope and spectrometer in the optical path.

By adjusting the position of diaphragm and the size of diaphragm, it can choose and a certain section of the luminous of luminous track is only allowed to pass through. According to measuring principle discussed earlier, the luminous signal of spatial domain has positive connection with delay time, and can quantitatively convert, The adjusting of delay time and the time of integration thus may be implemented by the selection of spatial domain, to realize that time-resolved spectrum is surveyed Amount.Spectrometer used uses existing business spectrometer, either the spectrum that monochromator and photodetector are constituted Instrument is also possible to the spectrograph of linear array or array image sensor composition.

The volume of actually used spectrometer may be larger, is not easy to the adjusting of position, thus this patent disclose it is a kind of excellent The spectral measurement device of choosing, which is characterized in that further include optical fiber other than including the measurement module, diaphragm, spectrometer of front And fiber collimating lenses.

Wherein behind fiber collimating lenses aligming microscope object lens, the sample for will transmit through dichroscope, which shines, to be focused Into optical fiber, then by optical fiber, optical signal is imported into the light inlet of spectrometer；Diaphragm be located at dichroscope in the optical path and Between collimation lens.

According to above-mentioned all devices, in order to reduce scattering light interference, a kind of preferred device is as follows: dichroscope and detection There is bandpass filter between device, selectively penetrates the light for needing the wave band detected.

Spectrum and service life, this patent in order to measure luminous simultaneously are disclosed directly below preferred embodiment:

It further include diaphragm, half-reflecting half mirror, optical fiber, fiber collimating lenses, spectrum including measurement module belonging to front Instrument, imaging sensor, lens；

Wherein, the light projected from measurement module passes through diaphragm, is radiated on half-reflecting half mirror, a part reflection, and in addition one Fractional transmission, in this two-way light, wherein all the way by lens focus on imaging sensor, for being imaged and lifetime measurement, separately Light is coupled in optical fiber by fiber collimating lenses all the way outside, can detecting light spectrum by the other one section of connection spectrometer of the optical fiber.

In order to realize multiple spot while measure, the pin hole in module can be replaced with pinhole array.

Detailed description of the invention

Fig. 1 is a kind of lifetime measurement device, wherein 101 be light source, 102 be pin hole, and 103 be dichroscope, and 104 be object Mirror, 105 be motor, and 106 be turntable, and 107 be imaging sensor, and 108 be camera lens.

Fig. 2 is the schematic diagram of lifetime measurement, wherein 201 be excitation point, 202 be the track that shines.

Fig. 3 is decay of luminescence curve synoptic diagram.

Fig. 4 is a kind of lifetime measurement device, wherein 101 be light source, 102 be pin hole, and 103 be dichroscope, and 105 be electricity Machine, 106 be turntable, and 107 be imaging sensor, and 108 be camera lens, and 109 be micro objective, and 110 be optical filter.

Fig. 5 is a kind of service life and time resolved spectroscopy measuring device, wherein 101 be light source, 102 be pin hole, 103 for two to Look mirror, 105 be motor, and 106 be turntable, and 107 be imaging sensor, and 108 be camera lens, and 109 be micro objective, and 111 be diaphragm, 112 be half-reflecting half mirror, and 113 be fiber collimating lenses, and 114 be optical fiber, and 115 be spectrometer.

Fig. 6 is a kind of measurement module, wherein 101 be light source, 102 be pin hole, and 103 be dichroscope, and 105 be motor, 109 It is turntable for micro objective, 116,117 be light hole, and 118 be miniature light sources, and 119 be optical detector.

Fig. 7 is a kind of measurement module, wherein 101 be light source, 102 be pin hole, and 103 be dichroscope, and 105 be motor, 109 It is turntable for micro objective, 120,121 be gear wheel, and 122 be pinion gear.

Specific embodiment

In order to illustrate the principle of the present invention and its advantage, the present invention is made below by specific embodiment further Illustrate, its object is to help to better understand the contents of the present invention, but these specific embodiments do not limit in any way Protection scope of the present invention.

Embodiment 1, a kind of lifetime measurement module and device

As shown in Fig. 1, the light that light source 101 exports is reflected through needle passing hole 102, then by dichroscope 103, by object lens 104 focus on turntable 106 a bit, and sample is covered on turntable, when motor 105 drives turntable rotation, the delay hair of sample Light can discharge in the region except excitation point, and a part, which shines, can pass through object lens 104, dichroscope 103, then be gathered by camera lens 108 Coke is in imaging sensor 107.

Wherein light source 101, pin hole 102, dichroscope 103, object lens 104, motor 105, turntable 106 constitute measurement module, The module is used cooperatively with imaging sensor 107 and camera lens 108 in this embodiment.

During lifetime measurement:

Motor driven turntable uniform rotation, as shown in Fig. 2；

Sample is fixed on turntable, thus sample can be rotated with turntable；

From the light focusing and a point on turntable that pin hole penetrates, as shown in Fig. 2, which is referred to as excitation point 201；

Sample is excited by excitation point, and then radiative relaxation discharges photon, and wherein the photon of delayed radiation can excite It is discharged except point, thus forms luminous track 202 as shown in Fig. 2, which is arc, in uniform rotation condition Under, the arc length of track is directly proportional to delay time；

The revolving speed of motor can pass through circuit control, it is known that motor speed, so that it may calculate each luminous point on track 202 Delay time；

It is taken pictures by imaging sensor to track, it can obtain the relative luminance of each luminous point；

Decay of luminescence curve can be obtained to delay time mapping with light emission luminance, as shown in Fig. 3, according to this field Known formula and method can obtain luminous life value to curve matching.

The basic principle of this method and previous patent (CN201711110647.X) are consistent.

Since the pixel of imaging sensor is limited, i.e. the luminous point of finite number can only be accommodated on an image, because And object lens magnification is higher, then the visual field is smaller, then temporal resolution is higher.Relative to patent before (CN201711110647.X), the visual field can be put more than several ten times larger using object lens, even up to the diffraction pole of optical imagery Limit, thus temporal resolution can be improved tens times or more.In conjunction with the use of high-speed motor, the luminous longevity of nanosecond can be measured Life has significant advantage.

In addition, adjusting the horizontal position of imaging sensor 107 and camera lens 108, it can choose suitable area of visual field and carry out Observation can avoid scattering light and non-delayed luminous interference.As shown in Fig. 2,2 regions have been enclosed on turntable, represent figure The visual field observed under different level position as sensor.

Wherein the visual field 1 includes excitation point, and the light of excitation point may be such that background increases, be unfavorable for seeing around being scattered in Examine the sample of dim light.By adjusting the horizontal position of imaging sensor, observation area can be made to move on at the visual field 2, kept away in the visual field 2 Excitation point has been opened, thus there is lower background.

Attached drawing 3 represents the luminous intensity of sample with the variation of delay time, and wherein the brightness change of A period represents view Luminous track in open country 1, the brightness change of B period represent the luminous track in the visual field 2.

According to similar principle, the tilt angle of adjustable dichroscope 103, or adjustment light source 101 and pin hole 102 Position, focus on excitation point except the observation visual field, scattering light and non-delayed luminous interference can also be reduced.

Embodiment 2, a kind of lifetime measurement device

As shown in Fig. 4, the light that light source 101 exports is reflected through needle passing hole 102, then by dichroscope 103, by microscope Object lens 109 focus on turntable 106 a bit, and sample is covered on turntable, and when motor 105 drives turntable rotation, sample prolongs Tardy light can discharge in the region except excitation point, and a part shines can be by micro objective 109, dichroscope 103 and filter Mating plate 110, then focused in imaging sensor 107 by camera lens 108.

According to the emission wavelength of specific sample, suitable optical filter 110 can choose, such as band logical, long wave are logical, short-pass To reduce scattering light interference.

The principle and embodiment 1 that the device measures the service life are unanimously.

Embodiment 3, a kind of service life and time resolved spectroscopy measuring device

As shown in Fig. 5, the light that light source 101 exports is reflected through needle passing hole 102, then by dichroscope 103, by microscope Object lens 109 focus on turntable 106 a bit, and sample is covered on turntable, and when motor 105 drives turntable rotation, sample prolongs Tardy light can discharge in the region except excitation point, and a part, which shines, can pass through micro objective 109, dichroscope 103, from light Door screen 111 projects, and then, half-reflecting half mirror 112 penetrates a part of this light beam, a part reflection, wherein the light quilt penetrated Camera lens 108 focuses in imaging sensor 107, and the light of reflection enters spectrometer by fiber optic collimator mirror 113 and optical fiber 114 In 115.

The principle and embodiment 1 that the device measures the service life are unanimously.

Wherein the size of diaphragm 111 and horizontal position are adjustable, to adjust size and the region in the visual field, are shone with choosing A certain section in track, it is used for service life and spectral measurement.Wherein obtained spectrum represents all hairs on the luminous track of the section Total spectrum of luminous point, that is, be similar to time-gated spectrum, delay time and gate duration can according to luminous point away from Arc length from excitation point is calculated.

In addition, half-reflecting half mirror 112 can be replaced by the optical filter of other transflection ratios.

Embodiment 4, a kind of measurement module

As shown in Fig. 6, the light that light source 101 exports is reflected through needle passing hole 102, then by dichroscope 103, by microscope Object lens 109 focus on turntable 116 a bit, there is light passing sky 117 on turntable 116, and the light that small-sized light source 118 projects passes through light passing Hole 117 is radiated on optical detector 119.Optical detector is selected from photodiode, can connect with oscillograph, for accurate in real time Motor speed is measured, accurately to calculate the relationship of arc length and delay time.

Embodiment 5, a kind of measurement module

As shown in Fig. 7, the light that light source 101 exports is reflected through needle passing hole 102, then by dichroscope 103, by microscope Object lens 109 focus on turntable 120 a bit, and turntable 120 connects with 122 axis of pinion gear below, pinion gear 122 and gear wheel 121 engagements, gear wheel 121 and 105 axis of motor connect.

In the method for lifetime measurement, the effect of motor is to provide high revolving speed, keeps the linear velocity of sample as high as possible, from In the structure of instrument, turntable and micro sample are very small to the load of motor, therefore, it is possible to be significantly improved by gear combination Revolving speed further increases temporal resolution.

The module is equally applicable to the measurement of lifetime measurement and time resolved spectroscopy.

Claims (10)

1. a kind of measurement module, it is characterised in that: the module includes motor, turntable, object lens, dichroscope, pin hole, light source；

The light that wherein light source projects is reflected by dichroscope, is focused on turntable using object lens through needle passing hole；Turntable is by motor Driving rotation；Sample to be tested is placed on turntable, and the fluorescence or phosphorescence issued is after object lens, can be by through dichroscope Detection.

2. measurement module as described in claim 1, it is characterised in that: light source is selected from collimated light source.

3. measurement module as described in claim 1, it is characterised in that: object lens are selected from micro objective.

4. measurement module as described in claim 1, it is characterised in that: turntable is by motor shaft even driving rotation.

5. measurement module as described in claim 1, it is characterised in that: turntable and gear shaft connect, motor and another gear shaft Even, and the gear on motor is engaged with the gear on turntable.

6. measurement module as described in claim 1, it is characterised in that: have light hole on turntable, in addition, module further includes one Miniature light sources and optical detector；Wherein, miniature light sources are selected from LED or laser light source, and optical detector is selected from photodiode, photoelectricity Triode, avalanche diode；

Also, the positional relationship of above-mentioned miniature light sources and detector and turntable are as follows: the light that light source issues can pass through turntable Light hole enters optical detector, i.e. detector is used to detect the light by light hole.

7. a kind of lifetime measurement device, which is characterized in that including any one module in such as claim 1 to 6, and including image Sensor and corresponding lens；Also, the luminous of sample is passed by object lens, through dichroscope, by lens focus in image In sensor.

8. a kind of spectral measurement device, which is characterized in that including any one module in such as claim 1 to 6, and including diaphragm And spectrometer；

Wherein it is luminous to be used to detect the sample through dichroscope for spectrometer, i.e. the light inlet of spectrometer is against dichroscope and object The rear of mirror；Diaphragm is located between the dichroscope and spectrometer in the optical path.

9. spectral measurement device as claimed in claim 8, which is characterized in that further include optical fiber and fiber collimating lenses；

Wherein behind fiber collimating lenses aligming microscope object lens, the sample for will transmit through dichroscope, which shines, focuses on light In fibre, then by optical fiber, optical signal is imported into the light inlet of spectrometer；Diaphragm is located at dichroscope and collimation in the optical path Between lens.

10. a kind of spectrum and lifetime measurement device, it is characterised in that: including any one module in such as claim 1 to 6, also Including diaphragm, half-reflecting half mirror, optical fiber, fiber collimating lenses, spectrometer, imaging sensor, lens；

Wherein, the light projected from measurement module passes through diaphragm, is radiated on half-reflecting half mirror, a part reflection, another part Transmission, in this two-way light, wherein all the way by lens focus on imaging sensor, for being imaged and lifetime measurement, in addition one Road light is coupled in optical fiber by fiber collimating lenses, can detecting light spectrum by the other one section of connection spectrometer of the optical fiber.