CN207908242U - Consumptive material and automation equipment for extracting bioactive substance - Google Patents
Consumptive material and automation equipment for extracting bioactive substance Download PDFInfo
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- CN207908242U CN207908242U CN201820108038.4U CN201820108038U CN207908242U CN 207908242 U CN207908242 U CN 207908242U CN 201820108038 U CN201820108038 U CN 201820108038U CN 207908242 U CN207908242 U CN 207908242U
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Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model provides the consumptive material and automation equipment for extracting bioactive substance.Consumptive material includes sample reaction part and the multiple tubular parts for being hermetically preinstalled with reagent, sample reaction part includes the airtight chamber that various reactions occur, fill the first hole of sample, multiple the second holes for receiving tubular part, discharge the third hole of waste liquid, collect the 4th hole of bioactive substance, first hole, second hole, third hole, 4th hole each leads to airtight chamber and is sealed, one end of multiple tubular parts is equipped with the piston that with its inner wall sealing engages and can slide wherein, the other end is equipped with reagent exit, and the reagent that at least one of multiple tubular parts are pre-installed includes the particle that is magnetic.Using the consumptive material and device of the utility model come when extracting bioactive substance, the mixing of liquid is realized by airtight chamber of the rotational oscillation equipped with the liquid, is avoided the splashing of liquid and the formation of aerosol, is eliminated the possibility of cross contamination.
Description
Technical field
The utility model is related to life sciences, and in particular, to a kind of consumptive material for extracting bioactive substance
And automation equipment.
Background technology
Modern clinic medical diagnosis on disease, medical jurisprudence calibrating, environmental microorganism detection, food safety detection, molecular biology etc.
Multiple fields are both needed to extraction bioactive substance, as cell, protein, nucleic acid (can be divided into ribonucleic acid (abbreviation RNA) and deoxidation
Ribonucleic acid (abbreviation DNA)) etc. bioactive substances.The extraction of bioactive substance more particularly to purification procedures, however,
Due to the complexity of biological sample, isolate and purify not a duck soup, especially multiple samples are carried out purifying or it is extremely difficult
, it not only needs to select suitable purification technique, but also workload is also especially big, it is difficult to meet current rapid development to high throughput
Sample extracts the demand of purifying.
It is biological section in recent years using paramagnetic particle method extraction bioactive substance active macromolecules especially as nucleic acid
It learns and the new and high technology of both nanometer material science unifications, this method uses surface of the nanotechnology to superparamagnetic nano particle
Improvement is carried out with after surface modification, is prepared into superparamagnetism silica nanometer magnetic bead.For example, the magnetic bead can on micro interface with
It identifies to nucleic acid specificity and efficiently combines.Using the superparamagnetism of silica nanoparticle, in Chaotropic salt (hydrochloric acid
Guanidine, guanidinium isothiocyanate etc.) and externally-applied magnetic field under the action of, can be from blood, animal tissue, food, pathogenic microorganism equal samples
DNA and RNA are isolated, clinical disease diagnosis, transfusion safety, Forensic Identification, environmental microorganism detection, food peace are can be applicable to
The multiple fields such as full inspection survey, molecular biology research.
It is above-mentioned to be further divided into suction method and magnetic again using paramagnetic particle method come the method for automatically extracting bioactive substance
Stick method.
For example, typically being mainly included the following steps that using the method for suction method extraction nucleic acid：Coordinated using pipetting channel
Suction nozzle injects lysate into the open container containing biological sample (such as blood, animal tissue, food, pathogenic microorganism),
It is blown and beaten and is mixed with suction nozzle；Magnetic-particle is injected into the container with suction nozzle and piping and druming is uniformly mixed, then make the container and magnet
It is close, so that magnetic-particle is adsorbed on the container inner wall, then waste liquid is sucked out with suction nozzle；Cleaning solution is added with suction nozzle and blows and beats
Mixing, to achieve the purpose that purged of impurities, then makes the container close with magnet, magnetic-particle is made to be adsorbed in the container
On wall, then waste liquid is sucked out (as needed, cleaning step can be repeated several times) with suction nozzle；Eluent is added with suction nozzle and blows and beats
Mixing, promote nucleic acid and magnetic-particle dissociation, after container is close with magnet, so that magnetic-particle is adsorbed on the container inner wall
On, then the eluent containing nucleic acid is sucked out with suction nozzle, obtains purified product, carries out subsequent operation.In each of suction method
In step, magnetic bead need to be retained in container always, thus suction nozzle cannot too close to the magnetic bead in container to prevent magnetic bead together with
Waste liquid is sopped up, this can undoubtedly influence elution efficiency and purity.
Another kind is bar magnet method developed in recent years, is documented in such as document US6447729, US6448092.It should
Method includes the steps that roughly the same with above-mentioned suction method, the difference is that by by magnetic devices (such as by casing and magnetic
Bar construction) liquid containing magnetic bead is immersed, so that magnetic bead is adsorbed on the magnetic devices surface and is transferred to next step and holds accordingly
In device, and the mixing purpose in each step is realized by up and down motion of the magnetic devices in open container.This method overcomes
The defect of above-mentioned suction method, but magnetic devices higher-orders of oscillation during moving up and down, easily occur liquid splash out it is gentle molten
Glue, this aerosol are that liquid or solid particle is evenly dispersed in the metastable suspension system formed in gas, are carried
Sample molecules, when the aerosol or liquid droplet for being dispersed in instrument internal enter other samples in, will result in the friendship of sample
Fork pollution.Using the instrument for extracting nucleic acid of bar magnet method due to technology and structural constraint, completely enclosed sample extraction ring cannot achieve
Border, can only be carried out disinfection by ultraviolet lamp processing or install additional powerful fan gas in machine is detached, mitigate cross contamination
Possibility, but can not fundamentally avoid the cross contamination between sample.
Utility model content
No cross contamination that the technical problem to be solved by the present invention is to provide one kind when extracting bioactive substance
Automation solutions.
One side according to the present utility model, the utility model provide a kind of consumption for extracting bioactive substance
Material, the consumptive material include sample reaction part and the multiple tubular parts for being hermetically preinstalled with reagent, wherein the sample reacting part
Part includes the first hole for the airtight chamber of various reactions to occur, for filling sample, multiple for receiving the tube
Second hole of part, the third hole for discharging waste liquid, the 4th hole for collecting bioactive substance, first hole, second
Hole, third hole, the 4th hole each lead to the airtight chamber and are sealed, one end of the multiple tubular part be equipped with
The piston that engages to its inner wall sealing and can slide wherein, the other end are equipped with reagent exit, and the multiple tubular part
At least one of the reagent pre-installed include the particle that is magnetic.
Using consumptive material as described above come when extracting bioactive substance, the mixing of liquid is to be equipped with to be somebody's turn to do by rotational oscillation
The airtight chamber of liquid avoids the splashing of liquid and the formation of aerosol in traditional suction method and bar magnet method come what is realized, from
And eliminate the possibility of cross contamination.In addition, be preinstalled in the tubular part of above-mentioned consumptive material reagent (for it is certain need to be in low temperature
The reagent of preservation, can by equipped with the reagent tubular part or entire consumptive material be saved previously in low temperature place), and it is corresponding
Automation equipment is used cooperatively, and the extraction process of bioactive substance can be fully automatically realized, so as to avoid manual operation
The error or mistake that may be brought, improve reliability, save cost of labor.
The consumptive material can also include one or more of following characteristics, can be used alone or be applied in combination：
The multiple tubular part is equipped with one end of reagent exit by it and is sealedly inserted into the multiple second hole,
To constitute an entirety with the sample reaction part.In this way, user is after obtaining the consumptive material, without voluntarily carrying out
The assembling of tubular part and sample reaction part, but directly the consumptive material of the one can be installed on matching used device
Carry out the extraction of bioactive substance.Obviously, integrated consumptive material uses more convenient, and it is real that user can be helped to save
Time before testing.
The examination in first hole, second hole, the third hole, the 4th hole and the multiple tubular part
Agent outlet is sealed by the first sealing-plug, the second sealing-plug, third sealing-plug, the 4th sealing-plug, the 5th sealing-plug respectively.According to this
Scheme, the tubular part and sample reaction part for being preinstalled with various reagents can individually be sold, to meet certain special reagents
It preserves and requires (such as in Cord blood).Using the split type consumptive material, come before extracting bioactive substance, user needs
Each sealing-plug is removed, and tubular part and sample reaction part are fitted together.Although with integral type consumptive material phase
Than split type consumptive material, which uses, shows slightly trouble, but its is more flexible, disclosure satisfy that more various demand.
The sample reaction part is hollow elongate member, including mutually substantially vertical bottom wall and longitudinal end wall, described
On longitudinal end wall, the third hole is arranged on the bottom wall setting of 4th hole.In this way, the biology extracted in discharge
When active material, sample reaction part can be placed in vertical state (i.e. its longitudinal axis is in vertical direction), and made with the
Liquid containing bioactive substance is more thoroughly discharged to by longitudinal end wall in four holes by the 4th hole downward, by means of pressure
In collection vessel.
The hollow elongate member further includes the roof substantially vertical and opposite with the bottom wall with longitudinal end wall,
First hole and the multiple second hole are arranged on the roof.Such more convenient bioactive substance of setting carries
The realization of various operating procedures during taking, the structure for the device being used cooperatively with the consumptive material also can be fairly simple.
The third hole and/or the covering of the 4th hole envelope, the film be chosen to only in applying vacuum or
In the case of pressure, the liquid of the film side could penetrate the film and reach the other side.In this way, using mating device profit
The waste collection conduit and biological active matter in third hole and the 4th hole when extracting bioactive substance, are inserted into the consumptive material
Matter collecting duct can not have to carry the film so that be directly possibly realized using the standard catheter of buying.
Other side according to the present utility model, the utility model additionally provide a kind of for extracting bioactive substance
Automation equipment, including：
The consumptive material fixing device being rotatably installed on holder, the sample reaction part of consumptive material as described above can
It is removedly fixed in the consumptive material fixing device；
Magnetic part, can be close to each other or separate with the consumptive material fixing device；
Pressing lever, the piston in tubular part for pressing the consumptive material, the pressing lever are fixed on elevating mechanism,
It can move up and down in the vertical direction；
Vacuum pressure device can generate vacuum and pressure and be connected to waste collection conduit；And
Control unit can control the rotary motion of the consumptive material fixing device, up and down motion of the pressing lever, described
The operation of relative motion and the vacuum pressure device between magnetic part and the consumptive material fixing device.
Above-mentioned automation equipment is used cooperatively with aforementioned consumptive material, and biological work can be fully automatically realized after injecting sample
The extraction of property substance, and avoid the possibility of cross contamination.Also, such automation equipment compact, operation is quiet,
It is of low cost, it is reliable for operation.
Above-mentioned automation equipment can also include one or more of following characteristics, and can be used alone or combine makes
With：
The consumptive material fixing device can be along being rotated clockwise and counterclockwise to any angle.In this way, consumptive material fixes dress
Rotational oscillation can be carried out with the consumptive material that is fixed thereon by setting, so as in each step that bioactive substance extracts to close
The indoor various liquid of closed chamber are sufficiently mixed, and various reactions is promoted fully to occur.In addition, needing that waste liquid and extraction is discharged
When good bioactive substance, the sample reaction part of consumptive material can be rotated to vertical state, so as in vacuum or pressure
Liquid inside more easily thoroughly being emptied under help.
The end in the third hole for being inserted into the sample reaction part of the waste collection conduit is covered with film, institute
It states film to be chosen to only in the case of applying vacuum or pressure, the liquid of the film side could be arrived through the film
Up to the other side.Such waste collection conduit is suitable for the case where third hole of consumptive material is without film.
The automation equipment further includes bioactive substance collecting duct, the bioactive substance collecting duct
One end is covered with film and the 4th hole for being inserted into the sample reaction part, wherein the film is chosen to only applying
In the case of vacuum or pressure, the liquid of the film side could penetrate the film and reach the other side, the bioactive substance
The other end of collecting duct leads to bioactive substance collection vessel.Such bioactive substance collecting duct is suitable for consumptive material
The 4th hole without film the case where.
The automation equipment further includes the closed waste liquid collection vessel being connect with the waste collection conduit.In this way,
Various waste liquids in experimentation can be all collected into a closed container, then carry out unified harmless treatment, subtract
It even is eliminated the pollution to environment less.
The consumptive material fixing device is equipped with opening so that when the sample reaction part of the consumptive material is fixed on the consumptive material
After in fixing device, the magnetic part being capable of bottom wall close by the opening or the fitting sample reaction part.This
The indoor magnetic-particle of closed chamber can be attracted to by sample when magnetic part is close or the bottom wall of fitting sample reaction part
On the inner wall of the chamber, magnetic-particle is prevented to be discharged together with liquid.
The consumptive material fixing device is equipped with temperature control device.The temperature control device can be used for heating or freeze, and e.g. set
Set in consumptive material fixation device surface or be built in heating plate therein, heating blanket, heater strip, semiconductor chilling plate etc..Have
The temperature control device, so that it may the indoor liquid of closed chamber to be heated or cooled in certain the step of needing temperature to adjust/control
Or heat preservation, this makes the automation equipment of the utility model have the scope of application of bigger.
In the present invention, term " bioactive substance " refers to the substance and material derived from life entity, including but not
It is limited to be derived from human body, animal body, plant, the nucleic acid in microbial body, albumen, polypeptide, metabolite and its derivative.
In the present invention, term " consumptive material " refers to the consumables for bioactive substance extraction process.
In the present invention, term " sample " refers to the ingredient for including the bioactive substance to be extracted, such as blood
Liquid, animal tissue, food, pathogenic microorganism.
In the present invention, term " reagent " refers to the liquid for bioactive substance extraction process, including but not
Be limited to lysate, biological enzyme, enzyme reaction system, magnetic-particle suspension, magnetic-particle and biomolecule combination liquid, cleaning solution,
The mixed liquor of eluent, PCR primer, sequence measuring joints, water, ethyl alcohol, isopropanol and aforesaid liquid.
In the present invention, term " tubular part " refers to component hollow, with a constant volume, and section can be with
It is any suitable shape.
In the present invention, term " magnetic part " refers to the magnetic component of any tool or device, such as permanent magnet
Or electromagnet.
Description of the drawings
The utility model is described in further detail below with reference to accompanying drawings.Skilled addressee readily understands that
It is that these attached drawings are intended solely for illustrative purposes, and are not intended to and are construed as limiting to the scope of protection of the utility model.Phase in figure
Same reference numeral indicates same or analogous component.For illustrative purposes, these figures and non-fully drawn to scale.
Fig. 1 is the exploded pictorial according to the consumptive material for extracting bioactive substance of the utility model one embodiment
Figure.
Fig. 2 is the diagrammatic top stereogram of sample reaction part in Fig. 1, in order to clearly show that its internal construction,
The roof of the sample reaction part is not shown in the figure.
Fig. 3 is the solid according to the automation equipment for extracting bioactive substance of the utility model one embodiment
Schematic diagram, wherein pressing lever are in initial position.
Fig. 4 is the solid according to the automation equipment for extracting bioactive substance of the utility model one embodiment
Schematic diagram, wherein pressing lever have been pressed into the tubular part of consumptive material.
Fig. 5 is the solid according to the automation equipment for extracting bioactive substance of the utility model one embodiment
Schematic diagram, wherein pressing lever have left the tubular part of consumptive material and consumptive material fixing device is rotated with the consumptive material being fixed thereon
Oscillation.
Fig. 6 is the solid according to the automation equipment for extracting bioactive substance of the utility model one embodiment
Schematic diagram, wherein rotational oscillation are completed, and consumptive material fixing device is with the consumptive material being fixed thereon close to magnetic part.
Fig. 7 is the vacuum according to the automation equipment for extracting bioactive substance of the utility model one embodiment
The operation principle schematic diagram of pressure apparatus.
Specific implementation mode
First, it is described according to the utility model one embodiment with reference to figure 1 and Fig. 2 for extracting bioactive substance
Consumptive material 100.As shown in Figure 1, consumptive material 100 includes sample reaction part 120 and multiple tubular parts 110, these tubular parts
It is inside all hermetically preinstalled with reagent, such as lysate, magnetic-particle suspension, Proteinase K, cleaning solution, eluent etc., wherein extremely
Reagent in a few tubular part contains magnetic-particle.In the exemplary embodiment shown in fig.1, there are 7 cylindrical pipes
Shape component 110, but it is to be understood that, those skilled in the art can according to actual demand come be arranged different number, no
The tubular part of similar shape.The upper end of each tubular part 110, which is equipped with, with its inner wall sealing to be engaged and can slide wherein
Piston (not shown), lower end be equipped with reagent exit 112.
As depicted in figs. 1 and 2, sample reaction part 120 includes for the airtight chamber 124 of various reactions to occur, is used for
Fill the first hole 114, multiple the second hole 113, the third holes for discharging waste liquid for receiving the tubular part of sample
127 and the 4th hole 125 for collecting bioactive substance, the first hole, the second hole, third hole, the 4th hole each lead to closed chamber
Room 124.In this embodiment, sample reaction part 120 is hollow elongate member, including longitudinal end wall 123 and longitudinal end wall
123 substantially vertical opposite roofs 121 and bottom wall 126, the first hole 114 and the second hole 113 are arranged on roof 121, the
Three holes 127 are arranged on bottom wall 126 (see Fig. 2), and the 4th hole 125 is arranged on longitudinal end wall 123.It should be understood that first
As hole, the second hole, third hole, the quantity in the 4th hole, position are not necessarily as shown in the figure, or even in certain embodiments,
Some of which can be same hole.
According to one embodiment modification, each tubular part 110 is sealedly inserted into corresponding second hole 113 by its lower end
In, to constitute an entirety with sample reaction part 120.In this case, consumptive material 100 is pre-assembled one whole
Body is additionally useful for discharging for filling the first hole 114 of sample in consumptive material using closing (such as with sealing-plug 115) is preceding needed
The third hole 127 of waste liquid and the 4th hole 125 for collecting bioactive substance are also required to close, so as not to dust, impurity etc. into
Enter in airtight chamber, pollutes reaction environment.
According to another embodiment variant, each tubular part 110 is not assembled in one in advance with sample reaction part 120
It rises, the memory requirement (for example, Cord blood) of certain special reagents can be met in this way.In this case, the first hole 114,
Second hole 113, third hole 127, the 4th hole 125 and each tubular part 110 reagent exit 112 be required to closing (such as
By means of sealing-plug), in order to avoid dust, impurity etc. enter in airtight chamber, pollute reaction environment.It is carried carrying out bioactive substance
Before taking experiment, only need to each tubular part 110 be inserted into corresponding second hole 113 in a predetermined sequence.
Third hole 127 and/or the 4th hole 125 can be covered with envelope, and the film is chosen to only in applying vacuum
Or in the case of pressure, the liquid of the film side could penetrate the film and reach the other side.Alternatively, third hole 127 and/
Or the 4th hole 125 can not also carry the film with above-mentioned property, but by film setting to the catheter end that will be inserted into the hole
Portion.Below in the description of the operation of automation equipment according to the present utility model, will more clearly understand the effect of the film.
It is described according to the utility model one embodiment below in conjunction with Fig. 3 to Fig. 6 for extracting biological active matter
The automation equipment 200 of matter comprising consumptive material fixing device 240, magnetic part 250, pressing lever 260, vacuum pressure device 300
(see Fig. 7) and control unit (not shown).
Wherein, consumptive material fixing device 240 is mounted on holder 230 and can surround its axis A along side clockwise or counterclockwise
To rotating to any angle.The sample reaction part 120 of consumptive material 100 as described above can be removably attached to consumptive material and fix
On device 240, in this embodiment, the flange 126 and consumptive material fixing device 240 that sample reaction part 120 passes through its bottom wall 122
The groove 241 at both ends coordinates and realizes this dismountable fixation, it should be appreciated that can also use any other suitable
Fixed form, and multiple consumptive materials 100 being removably attached in consumptive material fixing device 240, so as to simultaneously into
The multiple extraction experiments (show multiple pressing levers in Fig. 3-Fig. 6, but illustrate only a consumptive material 100) of row, improve working efficiency.
Preferably, consumptive material fixing device 240 is equipped with temperature control device.The heating device can be used for heating or freeze, example
Such as it is provided in consumptive material fixation device surface or is built in heating plate therein, heating blanket, heater strip, semiconductor chilling plate
Deng.There is the temperature control device, so that it may to be heated to the indoor liquid of closed chamber in certain the step of needing temperature to adjust/control
Or cooling or heat preservation, this makes the automation equipment of the utility model have the scope of application of bigger.
Pressing lever 260 is fixed on elevating mechanism 270, Z can be moved up and down along vertical direction, so as to when suitable
Machine presses the piston in the tubular part 110 of consumptive material 100, and the reagent pre-installed in it is pressed into the close of sample reaction part 120
In closed chamber room 124.
Magnetic part 250 is arranged in vertical wall 251, can be close to each other or separate with consumptive material fixing device 240.At this
In embodiment, holder 230 is arranged to consumptive material fixing device 240 and consumptive material 100 along the guide rail on horizontal base 231
232 move towards fixed magnetic part 250, but it is understood that, magnetic part 250 can also be arranged to and moved towards holder 230,
Or both move toward one another.Consumptive material fixing device 240 is equipped with opening 242 so that when the sample reaction part 120 of consumptive material 100 is solid
After being scheduled in consumptive material fixing device 240, magnetic part 250 can be close by opening 242 or fitting sample reaction part 120
Bottom wall 122 prevents magnetic-particle with liquid so that the magnetic-particle in airtight chamber 124 to be attracted on the inner wall of the chamber
Body is discharged together.It should be understood that opening 242 is not essential, such as consumptive material can be constituted by reasonable selection and fixed
The material and its thickness of device 240 reaches same purpose.
Vacuum pressure device 300 is a kind of device as needed that can be generated vacuum or generate pressure.As Fig. 7 is schematic
It shows, in one embodiment, 300 one side of vacuum pressure device is connected to waste collection conduit 210, is on the one hand connected to
Closed waste liquid collection vessel 280, and waste liquid collection vessel 280 is also connected to waste collection conduit 210 by another pipeline, and
And on the pipeline of connection waste collection conduit 210 and vacuum pressure device 300, in connection waste collection conduit 210 and waste liquid
Valve 310,320 is respectively equipped on the pipeline of collection vessel 280, they may, for example, be solenoid valve.Waste collection conduit 210
The end in the third hole 127 for being inserted into sample reaction part is covered with film 211 (see Fig. 1), which is chosen to only have
In the case of applying vacuum or pressure, the liquid of the film side could penetrate the film and reach the other side.Such waste collection
Conduit is suitable for the case where third hole 127 itself of consumptive material is without film.
The control unit (not shown) of automation equipment 200 can control the rotary motion of consumptive material fixing device 240, pressing
Relative motion between the up and down motion of bar 260, magnetic part 250 and consumptive material fixing device 240 and vacuum pressure device
300 operation.The control unit may, for example, be computer, the smart mobile phone etc. for being equipped with control program.
Automation equipment 200 can also include bioactive substance collecting duct 220 (see Fig. 1), which is used to insert
Film 221 can be covered with by entering 125 one end of the 4th hole of sample reaction part 120, which is chosen to only applying very
In the case of empty or pressure, the liquid of the film side could penetrate the film and reach the other side.Bioactive substance collecting duct 220
The other end lead to bioactive substance collection vessel 290 (such as centrifuge tube).Bioactive substance collection with film 221 is led
Pipe is suitable for the case where the 4th hole 125 itself of consumptive material is without film.
It describes how to utilize the consumptive material 100 and automation equipment according to one embodiment below in conjunction with Fig. 3 to Fig. 6
200 automatically extract bioactive substance.At this only to illustrate for extracting DNA in plasma sample, extraction experiment packet
Include following steps：
First, the consumptive material 100 suitable for the experiment is provided, which includes 6 tubular parts 110, is preinstalled with 10 respectively
Microlitre magnetic bead suspension, 15 microlitres of Proteinase K, 750 microlitres of lysate, 500 microlitres of the first cleaning solution, 500 microlitres
The second cleaning solution and 50 microlitres of eluent.Remove the sealing-plug 115 on the first hole 114 of the consumptive material, and by this
600 microlitres of plasma samples are added into the airtight chamber 124 of sample reaction part 120 for one hole 114, then cover sealing-plug 115
(see Fig. 3)；
The assembled consumptive material is installed in consumptive material fixing device 240 so that the third hole 127 of consumptive material is consolidated positioned at consumptive material
In the opening 242 for determining device 240, and waste collection conduit 210 and bioactive substance collecting duct 220 are inserted into consumptive material respectively
100 third hole 127 and the 4th hole 125, wherein the one end in the separate third hole 127 of waste collection conduit 210 is connected to closed
Waste liquid collection vessel 280, bioactive substance collecting duct 220 far from the 4th hole 125 one end be aligned biological active matter
Matter collection vessel 290 (being in this instance centrifuge tube)；
Open control unit, run corresponding with said extracted experiment program so that the automatic execution of automation equipment with
Lower step：
Pressing lever 260 moves downward, and extend into the tubular part containing magnetic bead suspension, presses piston therein, will
10 microlitres of magnetic bead suspension is pressed into the airtight chamber 124 of sample reaction part 120, and then pressing lever 260 is by similar
Operation 15 microlitres of Proteinase K, 750 microlitres of lysate are also pressed into airtight chamber 124 (see Fig. 4)；
Start rotation control mechanism, consumptive material fixing device 240 is made to surround axis A back and forth with consumptive material 100 fixed thereon
The rotational oscillation scheduled time is sufficiently mixed uniform (see Fig. 5) so as to the liquid in airtight chamber 124, at this time the DNA in sample
It should be specifically bound with magnetic bead；
Consumptive material fixing device 240 is set to be rotated to vertical state with consumptive material 100 fixed thereon, the consumptive material in vertical state
The opening 242 of fixing device 240 is located at lower section, and longitudinal end wall 123 with the 4th hole 125 of consumptive material 100 is downward；
Holder 230 is set to be moved towards magnetic part 250 along guide rail 232 with consumptive material fixing device 240 and consumptive material 100, directly
To magnetic part 250 by 242 close or fitting sample reaction part 120 the bottom walls 122 that are open, at this point, in airtight chamber 124
Magnetic bead be attracted on the inner wall of the chamber, stand 2 minutes after, allow vacuum pressure device 280 generate vacuum, make airtight chamber
Waste liquid in 124 is pumped to across the film 211 of 210 end of waste collection conduit in waste liquid collection vessel 280；
So that each device is returned to state shown in Fig. 3, by with operation like above, using pressing lever 260 by 500 microlitres
The first cleaning solution indentation airtight chamber 124 in, then as described above carry out rotational oscillation, empty waste liquid behaviour
Make；Then 500 microlitres of the second cleaning solution is directed to also to carry out similarly operating with the first cleaning solution；
Finally, 50 microlitres of eluent is pressed into airtight chamber 124 using pressing lever 260, then as described above
Method rotational oscillation predetermined time, the DNA being adsorbed at this time on magnetic bead should be detached from it, fall into eluent；Make consumption
Material fixing device 240 rotates to vertical state with consumptive material 100 fixed thereon and magnetic part 250 is made to be leaned on by opening 242
Close or fitting sample reaction part 120 bottom wall 122, at this point, the magnetic bead in airtight chamber 124 is attracted to the inner wall of the chamber
On, after standing 2 minutes, vacuum pressure device 280 is allowed to generate pressure, the eluent containing DNA in airtight chamber 124 is made to pass through life
The film 221 of 220 end of active substances collecting duct and enter in collection vessel 290 (see Fig. 6), subsequently grasped as needed
Make.
It should be understood that consumptive material according to the present utility model and automation equipment can be adapted for multiple biological activities object
Matter extraction experiment, those skilled in the art can repair to make adaptability to consumptive material and automation equipment according to actual needs
Change, for example, the reagent of the quantity of tubular part, size and Qi Nei prepackages, shape, volume of sample reaction part etc..
Attached drawing and non-limiting specific embodiment foregoing description describe the utility model.In order to instruct utility model former
Reason has simplified or has been omitted some conventional aspects.Those skilled in the art should understand that the modification from these embodiments is fallen
In the scope of the utility model.Those skilled in the art should understand that features described above can be combined in a variety of ways to form this reality
With novel multiple modifications.The utility model is not limited to above-mentioned specific embodiment as a result, and only by claim and they
Equivalent limit.
Claims (13)
1. a kind of for extracting the consumptive material (100) of bioactive substance, which is characterized in that including sample reaction part (120) and
Hermetically it is preinstalled with multiple tubular parts (110) of reagent, wherein the sample reaction part includes for various reactions to occur
Airtight chamber (124), the first hole (114) for filling sample, multiple for receiving the second hole of the tubular part
(113), the third hole (127) for discharging waste liquid, the 4th hole (125) for collecting bioactive substance, first hole,
Second hole, third hole, the 4th hole each lead to the airtight chamber and are sealed, and one end of the multiple tubular part is all provided with
There are the piston that with its inner wall sealing engages and can slide wherein, the other end to be equipped with reagent exit (112), and the multiple
The reagent that at least one of tubular part is pre-installed includes the particle that is magnetic.
2. consumptive material as described in claim 1, wherein the multiple tubular part is equipped with one end of reagent exit by it and seals
Ground is inserted into the multiple second hole, to constitute an entirety with the sample reaction part.
3. consumptive material as described in claim 1, wherein first hole, second hole, the third hole, the 4th hole
And the reagent exit of the multiple tubular part is respectively by the first sealing-plug (115), the second sealing-plug, third sealing-plug,
Four sealing-plugs, the sealing of the 5th sealing-plug.
4. consumptive material as claimed in any one of claims 1-3, wherein the sample reaction part is hollow elongate member, packet
Mutually substantially vertical bottom wall (122) and longitudinal end wall (123) are included, the 4th hole (125) is arranged on longitudinal end wall,
The third hole (127) is arranged on the bottom wall.
5. consumptive material as claimed in claim 4, wherein the hollow elongate member further includes substantially vertical with longitudinal end wall
And the roof (121) opposite with the bottom wall, first hole and the multiple second hole are arranged on the roof.
6. consumptive material as claimed in any one of claims 1-3, wherein the third hole and/or the covering of the 4th hole envelope,
The film is chosen to only in the case of applying vacuum or pressure, and the liquid of the film side could penetrate the film
Reach the other side.
7. a kind of automation equipment (200) for extracting bioactive substance, which is characterized in that including：
The consumptive material fixing device (240) being rotatably installed on holder (230), as described in any one of the preceding claims
The sample reaction part of consumptive material (100) can be removably attached in the consumptive material fixing device；
Magnetic part (250), can be close to each other or separate with the consumptive material fixing device；
Pressing lever (260), the piston in tubular part for pressing the consumptive material, the pressing lever are fixed on elevating mechanism
(270) it on, can move up and down in the vertical direction；
Vacuum pressure device can generate vacuum and pressure and be connected to waste collection conduit (210)；And
Control unit can control the rotary motion of the consumptive material fixing device, the up and down motion of the pressing lever, the magnetism
The operation of relative motion and the vacuum pressure device between component and the consumptive material fixing device.
8. automation equipment as claimed in claim 7, wherein the consumptive material fixing device can be along clockwise and counterclockwise
Rotate to any angle.
9. automation equipment as claimed in claim 7, wherein the waste collection conduit is reacted for being inserted into the sample
The end in the third hole of component be covered with film (211,
221), the film is chosen to only in the case of applying vacuum or pressure, the liquid ability of the film side
The other side is reached through the film.
10. automation equipment as claimed in any one of claims 7-9, wherein the automation equipment further includes biological work
Property matter collecting catheter, one end of the bioactive substance collecting duct is covered with film and for being inserted into the sample reaction part
The 4th hole, wherein the film is chosen to only in the case of applying vacuum or pressure, the liquid of the film side
The film could be penetrated and reach the other side, the other end of the bioactive substance collecting duct (220) leads to biological active matter
Matter collection vessel (290).
11. automation equipment as claimed in any one of claims 7-9, wherein the automation equipment further include with it is described
The closed waste liquid collection vessel (280) of waste collection conduit connection.
12. automation equipment as claimed in any one of claims 7-9, wherein the consumptive material fixing device is equipped with opening
(242) so that after the sample reaction part of the consumptive material is fixed in the consumptive material fixing device, the magnetic part can
Pass through close or the fitting sample reaction part the bottom wall that is open.
13. automation equipment as claimed in any one of claims 7-9, wherein the consumptive material fixing device is filled equipped with temperature control
It sets.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820108038.4U CN207908242U (en) | 2018-01-23 | 2018-01-23 | Consumptive material and automation equipment for extracting bioactive substance |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820108038.4U CN207908242U (en) | 2018-01-23 | 2018-01-23 | Consumptive material and automation equipment for extracting bioactive substance |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207908242U true CN207908242U (en) | 2018-09-25 |
Family
ID=63564423
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820108038.4U Withdrawn - After Issue CN207908242U (en) | 2018-01-23 | 2018-01-23 | Consumptive material and automation equipment for extracting bioactive substance |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207908242U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110068493A (en) * | 2018-01-23 | 2019-07-30 | 北京九州创新生物科技有限公司 | For extracting the consumptive material, automation equipment and method of bioactive substance |
| CN114570449A (en) * | 2022-04-26 | 2022-06-03 | 广州国家实验室 | Liquid transfer device and multi-path parallel liquid transfer device |
-
2018
- 2018-01-23 CN CN201820108038.4U patent/CN207908242U/en not_active Withdrawn - After Issue
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110068493A (en) * | 2018-01-23 | 2019-07-30 | 北京九州创新生物科技有限公司 | For extracting the consumptive material, automation equipment and method of bioactive substance |
| CN110068493B (en) * | 2018-01-23 | 2024-09-10 | 国源生命科学集团有限公司 | Consumable, automation device and method for extracting bioactive substances |
| CN114570449A (en) * | 2022-04-26 | 2022-06-03 | 广州国家实验室 | Liquid transfer device and multi-path parallel liquid transfer device |
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