CN207730667U

CN207730667U - A kind of real time fluorescent quantitative augmentation detection instrument

Info

Publication number: CN207730667U
Application number: CN201721712520.0U
Authority: CN
Inventors: 胡志刚
Original assignee: Suzhou Hehui Biotechnology Co Ltd
Current assignee: Suzhou Hehui Biotechnology Co Ltd
Priority date: 2017-12-11
Filing date: 2017-12-11
Publication date: 2018-08-14
Anticipated expiration: 2027-12-11

Abstract

The utility model is related to a kind of real time fluorescent quantitative augmentation detection instrument, including babinet, controller, being arranged in the turnover case lid in babinet upper end, the nucleic acid amplification module being separately positioned in babinet and fluoroscopic examination module；The nucleic acid amplification module includes the 96 hole heating plates for being sequentially placed and being bonded to each other from top to bottom, semiconductor chilling plate, heat conducting pipe, radiator, wind turbine；The fluoroscopic examination module includes rack, is separately positioned in rack UV IR filters and focus lamp are horizontally set on frame upper end and are arranged on turntable that the optical filter, two beam one end that circumferentially direction is uniformly placed and wavelength is different are arranged above turntable and the other end connect with 96 hole heating plates and extends to the incident optical beam and the output optical fiber beam, the light source and fluorescence detector that are separately positioned on rack lower end of each hole wall respectively by stepper motor driven turntable, multi-disc；The utility model can not only improve detection sensitivity, reduce manufacturing cost, and simple in structure, easy to use.

Description

A kind of real time fluorescent quantitative augmentation detection instrument

Technical field

The utility model is related to PCR amplification detection fields, refer in particular to a kind of real time fluorescent quantitative augmentation detection instrument.

Background technology

Nucleic acid amplification fluorescent detecting system is the reagent being irradiated to exciting light in sample cell, is then received out of sample cell The fluorescence that reagent moment inspires, to realize sample is qualitative and quantitatively detects system.

Exciting light is irradiated at the top of sample cell currently, existing nucleic acid amplification fluorescent detecting system is substantially, sample In pipe after reagent excitation fluorescence, then the fluorescence for receiving excitation is returned to through sample cell top, such as the prior art 201710286911.9 In disclose a kind of real-time fluorescence quantitative PCR gene magnification detector and have the disadvantages that in use：1, light source generates For light after semi-transparency type filter and optical filter, the spectrum for forming particular color passes through the sheet glass in through-hole to enter in reagent wells, Preset fluorescent material is absorbed into the light in reagent wells in reagent wells, the light that fluorescent material is sent out by through-hole through optical filter and In semi-transparency type filter transmission and reflection to fluorescence collector, since the fluorescence of excitation will again connect after the scattering at the top of reagent wells It receives, light intensity has greater loss, and the sensitivity of detection is caused to decline；2, hinge hole is matched with connecting hole so that fluoroscopic examination module Gene magnification module can be surrounded to overturn, flipped outward convenient for fluoroscopic examination module so that it is very laborious when replacement reagent, and turn over Turn number and is excessively easy to cause the reduction of fluoroscopic examination module precision；3, light source generate light after semi-transparency type filter, using The spectrum that optical filter forms particular color enters in reagent wells, device for revolving and driving rotation, and the optical filter of different colours is right successively Accurate reagent wells circumferentially are considerably increased and are manufactured into since each channel is required for setting semi-transparency type filter and optical filter This.

Utility model content

The utility model aim is to provide a kind of real time fluorescent quantitative augmentation detection for overcome the deficiencies in the prior art Instrument can not only put forward detection sensitivity, reduce manufacturing cost, and be simple in structure and convenient in use.

In order to achieve the above objectives, the technical solution adopted in the utility model is：A kind of real time fluorescent quantitative augmentation detection instrument, Including babinet, controller, be arranged babinet upper end and can flip outward case lid, be separately positioned in babinet and controller line connect The nucleic acid amplification module and fluoroscopic examination module connect；The nucleic acid amplification module include be placed in pedestal below case lid, on to Under be successively set on pedestal and be bonded to each other 96 hole heating plates, semiconductor chilling plate, heat conducting pipe, radiator, wind turbine； The fluoroscopic examination module includes rack, is separately positioned in rack UV-IR filters and focus lamp are horizontally set in rack It holds and the optical filtering that circumferentially direction is uniformly placed and wavelength is different is arranged on turntable by stepper motor driven turntable, multi-disc Piece, two beam one end are arranged above turntable and the other end connect with 96 hole heating plates and extends to entering for each hole wall respectively Penetrate fiber optic bundle and the output optical fiber beam, the light source and fluorescence detector that are separately positioned on rack lower end；The light that the light source is sent out according to It is secondary by after UV-IR filters, wherein a tablet filter, incident optical beam enter 96 hole heating plates, be tested reagent excitation Fluorescence enters fluorescence detector after passing sequentially through the output optical fiber beam, wherein another tablet filter, focus lamp.

Preferably, the rack include lower cover, be arranged lower cover upper end upper shell cover, be arranged in lower cover lower end Holder；The UV-IR filters and focus lamp are arranged on lower cover, and turntable is arranged between upper shell cover and lower cover, and two beams enter The one end and stepper motor for penetrating fiber optic bundle and the output optical fiber beam are separately positioned on upper shell cover, and light source and fluorescence detector are set respectively It sets on holder；The upper and lower ends of the turntable are respectively arranged with and upper shell cover and lower casing in the circumference that multiple optical filters are formed The annular retaining ring that lid gap is placed.

Preferably, the semiconductor chilling plate is provided with multi-disc, and arranged in arrays.

Preferably, horizontal positioned heating cover board is provided on the case lid above pedestal.

Preferably, on the case lid sealing ring that a circle is in rectangular-ambulatory-plane is additionally provided at the edge of heating cover board.

Preferably, it is additionally provided in the babinet and gives the switch of nucleic acid amplification module and fluoroscopic examination module for power supply electricity respectively Source.

Preferably, the both sides of the babinet are respectively arranged with radiator window.

Preferably, the light source is one kind in LED light, xenon lamp, halogen lamp or laser light source.

Preferably, the fluorescence detector is one kind in CCD detector, photodiode or PMT photomultipliers.

Due to the application of the above technical scheme, the utility model has following advantages compared with prior art：

Real time fluorescent quantitative augmentation detection instrument described in the utility model in hole wall site by that directly can realize fluorescence The phenomenon that excitation and reception, removing fluorescent scattering and light intensity decays, improve the detection sensitivity of fluorescence quantitative gene extender； And nucleic acid amplification module is set up directly below case lid, sample cell can directly be replaced by opening case lid, more convenient to use；And it is more The optical filter of piece different wave length is arranged on turntable simultaneously, it is only necessary to rotate turntable according to fluorescent dye and choose corresponding optical filter , it is not only simple in structure, purposes is wider, and can reduce manufacturing cost.

Description of the drawings

Technical solutions of the utility model are described further below in conjunction with the accompanying drawings：

Attached drawing 1 is the structural schematic diagram of real time fluorescent quantitative augmentation detection instrument described in the utility model；

Attached drawing 2 is that the utility model removes the structural schematic diagram after babinet and case lid；

Attached drawing 3 is the structural schematic diagram of fluoroscopic examination module in the utility model；

Attached drawing 4 is the sectional view of fluoroscopic examination module in the utility model；

Attached drawing 5 is the sectional view that the utility model amplifying nucleic acid expands module；

Attached drawing 6 is the light path schematic diagram of single hole in 96 hole heating plates in the utility model.

Wherein：1, babinet；11, radiator window；2, case lid；21, cover board is heated；22, sealing ring；3, nucleic acid amplification module；31、 Pedestal；32,96 hole heating plate；33, semiconductor chilling plate；34, heat conducting pipe；35, radiator；36, wind turbine；4, fluoroscopic examination Module；40, rack；401, lower cover；402, holder；403, upper shell cover；41, incident optical beam；42, the output optical fiber beam；43, turn Disk；431, annular retaining ring；44, optical filter；45, halogen lamp；46, CCD detector；47, stepper motor；48, UV-IR filters；49、 Focus lamp；5, Switching Power Supply；6, sample cell.

Specific implementation mode

Below in conjunction with the accompanying drawings and specific embodiment the utility model is described in further detail.

Attached drawing 1-6 is real time fluorescent quantitative augmentation detection instrument described in the utility model, including babinet 1, controller, setting 1 upper end of babinet and can flip outward case lid 2, be separately positioned on the nucleic acid amplification module 3 being connect with controller line in babinet 1 With fluoroscopic examination module 4；The nucleic acid amplification module 3 includes the pedestal 31 for being placed in 2 lower section of case lid, is successively set on from top to bottom On pedestal 31 and be bonded to each other 96 hole heating plates 32, semiconductor chilling plate 33, heat conducting pipe 34, radiator 35, wind turbine 36； The fluoroscopic examination module 4 includes the rack 40 being made of upper shell cover 403, lower cover 401, holder 402, is separately positioned on lower casing UV-IR filters 48 on lid 401 and focus lamp 49 are horizontally set between upper shell cover 403 and lower cover 401 and by stepping electricity The optical filter 44, two that circumferentially direction is uniformly placed and wavelength is different is arranged on turntable 43 in the turntable 43 of the driving of machine 47, multi-disc Shu Yiduan is arranged on upper shell cover 403 and the other end connect with 96 hole heating plates 32 and extends to entering for each hole wall respectively Penetrate fiber optic bundle 41 and the output optical fiber beam 42, the halogen lamp 45 that is separately positioned on holder 402 and CCD detector 46；The halogen The light that lamp 45 is sent out enters the heating of 96 holes after passing sequentially through UV-IR filters 48, wherein a tablet filter, incident optical beam 41 Plate 32, the fluorescence that reagent excites in detected sample quality control 6 pass sequentially through the output optical fiber beam 42, wherein another tablet filter, focus lamp Enter CCD detector 46 after 49.

Further, the upper and lower ends of the turntable 43 are respectively arranged with and upper shell cover in the circumference that multiple optical filters are formed 403 and 401 gap of lower cover place annular retaining ring 431, play the interference of light CCD for preventing halogen lamp 45 from sending out every light action Detector 46 receives fluorescence, causes to measure inaccurate.

Further, the semiconductor chilling plate 33 is provided with multi-disc, and arranged in arrays, with it is every it is a piece of be a region, Realize multi-region temperature control technique, can effectively improve the uniformity of accuracy of temperature control and temperature, and can gradient setting, purposes is wider.

Further, horizontal positioned heating cover board 21 is provided on the case lid 2 in the top of pedestal 31 so that temperature is more Add uniformly.

Further, it is additionally provided with the sealing ring 22 that a circle is in rectangular-ambulatory-plane at the edge of heating cover board 21 on the case lid 2, prevented Only reagent volatilizees when heated, and amplification efficiency reduces.

Further, it is additionally provided in the babinet 1 and is opened respectively to what nucleic acid amplification module 3 and fluoroscopic examination module 4 were powered Powered-down source 5 avoids powering off suddenly during the work time, needs to detect again, not only influence testing result, also greatly increase inspection Survey the time.

Further, the both sides of the babinet 1 are respectively arranged with radiator window 11, play the role of heat dissipation ventilation, reinforce heat dissipation effect Fruit.

The specific application example that the above is only the utility model does not constitute the scope of protection of the utility model any limit System.Any technical scheme formed by adopting equivalent transformation or equivalent replacement, all fall within the utility model rights protection scope it It is interior.

Claims

1. a kind of real time fluorescent quantitative augmentation detection instrument, it is characterised in that：Including babinet, controller, setting in babinet upper end and The case lid that can flip outward is separately positioned on the nucleic acid amplification module being connect with controller line in babinet and fluoroscopic examination module； The nucleic acid amplification module includes the pedestal being placed in below case lid, be successively set on pedestal and be bonded to each other from top to bottom nine 16 hole heating plates, semiconductor chilling plate, heat conducting pipe, radiator, wind turbine；The fluoroscopic examination module includes rack, sets respectively It sets UV-IR filters and focus lamp in rack, be horizontally set on frame upper end and by stepper motor driven turntable, multi-disc It is above turntable and another that the optical filter that circumferentially direction is uniformly placed and wavelength is different, the setting of two beam one end are set on turntable It holds the incident optical beam for connecting and extending to each hole wall with 96 hole heating plates respectively and the output optical fiber beam, be separately positioned on The light source and fluorescence detector of rack lower end；The light that the light source is sent out pass sequentially through UV-IR filters, a wherein tablet filter, Enter 96 hole heating plates after incident optical beam, the fluorescence of tested reagent excitation passes sequentially through the output optical fiber beam, wherein another Enter fluorescence detector after tablet filter, focus lamp.

2. real time fluorescent quantitative augmentation detection instrument according to claim 1, it is characterised in that：The rack includes lower casing Cover, be arranged lower cover upper end upper shell cover, holder in lower cover lower end is set；The UV-IR filters and focus lamp setting On lower cover, turntable is arranged between upper shell cover and lower cover, one end and the step of two beam incident optical beams and the output optical fiber beam Stepper motor is separately positioned on upper shell cover, and light source and fluorescence detector are separately positioned on holder；The upper and lower ends of the turntable The annular retaining ring with upper shell cover and the placement of lower cover gap is respectively arranged in the circumference that multiple optical filters are formed.

3. real time fluorescent quantitative augmentation detection instrument according to claim 1 or 2, it is characterised in that：The semiconductor refrigerating Piece is provided with multi-disc, and arranged in arrays.

4. real time fluorescent quantitative augmentation detection instrument according to claim 3, it is characterised in that：In pedestal on the case lid Top is provided with horizontal positioned heating cover board.

5. real time fluorescent quantitative augmentation detection instrument according to claim 4, it is characterised in that：In heating cover on the case lid The edge of plate is additionally provided with the sealing ring that a circle is in rectangular-ambulatory-plane.

6. real time fluorescent quantitative augmentation detection instrument according to claim 1, it is characterised in that：It is additionally provided in the babinet The Switching Power Supply of nucleic acid amplification module and fluoroscopic examination module for power supply is given respectively.

7. real time fluorescent quantitative augmentation detection instrument according to claim 1, it is characterised in that：Distinguish the both sides of the babinet It is provided with radiator window.

8. real time fluorescent quantitative augmentation detection instrument according to claim 1, it is characterised in that：The light source is LED light, xenon One kind in gas lamp, halogen lamp or laser light source.

9. real time fluorescent quantitative augmentation detection instrument according to claim 1, it is characterised in that：The fluorescence detector is One kind in CCD detector, photodiode or PMT photomultipliers.